J. Rack, Antonio Ayala Ariza, Bryon S. Drown, Callum Henfrey, Edward J. Bartlett, T. Shirai, P. Hergenrother, I. Ahel
Summary Protein ADP-ribosylation is a highly dynamic post-translational modification. The rapid turnover is achieved, among others, by ADP-(ribosyl)hydrolases (ARHs), an ancient family of enzymes that reverses this modification. Recently ARHs came into focus due to their role as regulators of cellular stresses and tumor suppressors. Here we present a comprehensive structural analysis of the enzymatically active family members ARH1 and ARH3. These two enzymes have very distinct substrate requirements. Our data show that binding of the adenosine ribose moiety is highly diverged between the two enzymes, whereas the active sites harboring the distal ribose closely resemble each other. Despite this apparent similarity, we elucidate the structural basis for the selective inhibition of ARH3 by the ADP-ribose analogues ADP-HPD and arginine-ADP-ribose. Together, our biochemical and structural work provides important insights into the mode of enzyme-ligand interaction, helps to understand differences in their catalytic behavior, and provides useful tools for targeted drug design.
{"title":"ADP-ribosylserine hydrolase ARH3 of Latimeria chalumnae in complex with ADP-ribosyl-L-arginine","authors":"J. Rack, Antonio Ayala Ariza, Bryon S. Drown, Callum Henfrey, Edward J. Bartlett, T. Shirai, P. Hergenrother, I. Ahel","doi":"10.2210/PDB6HH4/PDB","DOIUrl":"https://doi.org/10.2210/PDB6HH4/PDB","url":null,"abstract":"Summary Protein ADP-ribosylation is a highly dynamic post-translational modification. The rapid turnover is achieved, among others, by ADP-(ribosyl)hydrolases (ARHs), an ancient family of enzymes that reverses this modification. Recently ARHs came into focus due to their role as regulators of cellular stresses and tumor suppressors. Here we present a comprehensive structural analysis of the enzymatically active family members ARH1 and ARH3. These two enzymes have very distinct substrate requirements. Our data show that binding of the adenosine ribose moiety is highly diverged between the two enzymes, whereas the active sites harboring the distal ribose closely resemble each other. Despite this apparent similarity, we elucidate the structural basis for the selective inhibition of ARH3 by the ADP-ribose analogues ADP-HPD and arginine-ADP-ribose. Together, our biochemical and structural work provides important insights into the mode of enzyme-ligand interaction, helps to understand differences in their catalytic behavior, and provides useful tools for targeted drug design.","PeriodicalId":9772,"journal":{"name":"Chemistry & biology","volume":"151 1","pages":"1533"},"PeriodicalIF":0.0,"publicationDate":"2018-11-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84296045","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2015-12-17DOI: 10.1016/j.chembiol.2015.10.014
M. Riemersma, D. S. Froese, Walinka van Tol, U. Engelke, J. Kopeć, Monique van Scherpenzeel, A. Ashikov, T. Krojer, Frank von Delft, M. Tessari, A. Buczkowska, E. Swiezewska, L. T. Jae, T. Brummelkamp, H. Manya, T. Endo, Hans van Bokhoven, W. Yue, D. Lefeber
{"title":"Human ISPD Is a Cytidyltransferase Required for Dystroglycan O-Mannosylation.","authors":"M. Riemersma, D. S. Froese, Walinka van Tol, U. Engelke, J. Kopeć, Monique van Scherpenzeel, A. Ashikov, T. Krojer, Frank von Delft, M. Tessari, A. Buczkowska, E. Swiezewska, L. T. Jae, T. Brummelkamp, H. Manya, T. Endo, Hans van Bokhoven, W. Yue, D. Lefeber","doi":"10.1016/j.chembiol.2015.10.014","DOIUrl":"https://doi.org/10.1016/j.chembiol.2015.10.014","url":null,"abstract":"","PeriodicalId":9772,"journal":{"name":"Chemistry & biology","volume":"57 1","pages":"1643-52"},"PeriodicalIF":0.0,"publicationDate":"2015-12-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73787682","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2015-12-17DOI: 10.1016/j.chembiol.2015.10.010
G. Ortega, T. Diercks, Ó. Millet
{"title":"Halophilic Protein Adaptation Results from Synergistic Residue-Ion Interactions in the Folded and Unfolded States.","authors":"G. Ortega, T. Diercks, Ó. Millet","doi":"10.1016/j.chembiol.2015.10.010","DOIUrl":"https://doi.org/10.1016/j.chembiol.2015.10.010","url":null,"abstract":"","PeriodicalId":9772,"journal":{"name":"Chemistry & biology","volume":"70 1","pages":"1597-607"},"PeriodicalIF":0.0,"publicationDate":"2015-12-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73711460","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2015-12-17DOI: 10.1016/j.chembiol.2015.11.004
Virginia del Solar, Darleny Y. Lizardo, Nasi Li, Jerod J. Hurst, Christopher J. Brais, G. E. Atilla‐Gokcumen
{"title":"Differential Regulation of Specific Sphingolipids in Colon Cancer Cells during Staurosporine-Induced Apoptosis.","authors":"Virginia del Solar, Darleny Y. Lizardo, Nasi Li, Jerod J. Hurst, Christopher J. Brais, G. E. Atilla‐Gokcumen","doi":"10.1016/j.chembiol.2015.11.004","DOIUrl":"https://doi.org/10.1016/j.chembiol.2015.11.004","url":null,"abstract":"","PeriodicalId":9772,"journal":{"name":"Chemistry & biology","volume":"35 1","pages":"1662-70"},"PeriodicalIF":0.0,"publicationDate":"2015-12-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82525065","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2015-12-17DOI: 10.1016/j.chembiol.2015.10.015
M. Vivoli, M. Isupov, R. Nicholas, A. Hill, Andrew E. Scott, P. Kosma, J. Prior, N. Harmer
{"title":"Unraveling the B. pseudomallei Heptokinase WcbL: From Structure to Drug Discovery","authors":"M. Vivoli, M. Isupov, R. Nicholas, A. Hill, Andrew E. Scott, P. Kosma, J. Prior, N. Harmer","doi":"10.1016/j.chembiol.2015.10.015","DOIUrl":"https://doi.org/10.1016/j.chembiol.2015.10.015","url":null,"abstract":"","PeriodicalId":9772,"journal":{"name":"Chemistry & biology","volume":"30 1","pages":"1622 - 1632"},"PeriodicalIF":0.0,"publicationDate":"2015-12-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88285102","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2015-12-17DOI: 10.1016/j.chembiol.2015.10.011
Zibo Zhao, Lu Wang, Youngeun Jung, Ikyon Kim, R. Tan, Wei Xu
{"title":"Reciprocal Regulation of ERα and ERβ Stability and Activity by Diptoindonesin G.","authors":"Zibo Zhao, Lu Wang, Youngeun Jung, Ikyon Kim, R. Tan, Wei Xu","doi":"10.1016/j.chembiol.2015.10.011","DOIUrl":"https://doi.org/10.1016/j.chembiol.2015.10.011","url":null,"abstract":"","PeriodicalId":9772,"journal":{"name":"Chemistry & biology","volume":"279 1","pages":"1608-21"},"PeriodicalIF":0.0,"publicationDate":"2015-12-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75117648","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2015-12-17DOI: 10.1016/j.chembiol.2015.10.012
Sheng Huang, S. Elsayed, Meinan Lv, J. Tabudravu, M. Rateb, Roland Gyampoh, K. Kyeremeh, R. Ebel, M. Jaspars, Z. Deng, Yi-Zun Yu, Hai Deng
{"title":"Biosynthesis of Neocarazostatin A Reveals the Sequential Carbazole Prenylation and Hydroxylation in the Tailoring Steps.","authors":"Sheng Huang, S. Elsayed, Meinan Lv, J. Tabudravu, M. Rateb, Roland Gyampoh, K. Kyeremeh, R. Ebel, M. Jaspars, Z. Deng, Yi-Zun Yu, Hai Deng","doi":"10.1016/j.chembiol.2015.10.012","DOIUrl":"https://doi.org/10.1016/j.chembiol.2015.10.012","url":null,"abstract":"","PeriodicalId":9772,"journal":{"name":"Chemistry & biology","volume":"41 1","pages":"1633-42"},"PeriodicalIF":0.0,"publicationDate":"2015-12-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81672470","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2015-12-17DOI: 10.1016/j.chembiol.2015.10.016
T. Kawakami, K. Ogawa, N. Goshima, T. Natsume
{"title":"DIVERSE System: De Novo Creation of Peptide Tags for Non-enzymatic Covalent Labeling by In Vitro Evolution for Protein Imaging Inside Living Cells.","authors":"T. Kawakami, K. Ogawa, N. Goshima, T. Natsume","doi":"10.1016/j.chembiol.2015.10.016","DOIUrl":"https://doi.org/10.1016/j.chembiol.2015.10.016","url":null,"abstract":"","PeriodicalId":9772,"journal":{"name":"Chemistry & biology","volume":"10 1","pages":"1671-9"},"PeriodicalIF":0.0,"publicationDate":"2015-12-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88586598","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2015-12-17DOI: 10.1016/j.chembiol.2015.11.005
T. M. Moon, N. Tykocki, Jessica L. Sheehe, B. W. Osborne, W. Tegge, J. Brayden, W. Dostmann
{"title":"Synthetic Peptides as cGMP-Independent Activators of cGMP-Dependent Protein Kinase Iα.","authors":"T. M. Moon, N. Tykocki, Jessica L. Sheehe, B. W. Osborne, W. Tegge, J. Brayden, W. Dostmann","doi":"10.1016/j.chembiol.2015.11.005","DOIUrl":"https://doi.org/10.1016/j.chembiol.2015.11.005","url":null,"abstract":"","PeriodicalId":9772,"journal":{"name":"Chemistry & biology","volume":"52 1","pages":"1653-61"},"PeriodicalIF":0.0,"publicationDate":"2015-12-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86010767","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2015-12-17DOI: 10.1016/j.chembiol.2015.10.009
Sonali Srivastava, Sonali Srivastava, S. Chaudhary, Lipi Thukral, C. Shi, R. Gupta, Radhika Gupta, Radhika Gupta, K. Priyadarshan, A. Vats, A. S. Haque, R. Sankaranarayanan, Vivek T. Natarajan, Vivek T. Natarajan, Rakesh Sharma, C. Aldrich, R. Gokhale, R. Gokhale
{"title":"Unsaturated Lipid Assimilation by Mycobacteria Requires Auxiliary cis-trans Enoyl CoA Isomerase.","authors":"Sonali Srivastava, Sonali Srivastava, S. Chaudhary, Lipi Thukral, C. Shi, R. Gupta, Radhika Gupta, Radhika Gupta, K. Priyadarshan, A. Vats, A. S. Haque, R. Sankaranarayanan, Vivek T. Natarajan, Vivek T. Natarajan, Rakesh Sharma, C. Aldrich, R. Gokhale, R. Gokhale","doi":"10.1016/j.chembiol.2015.10.009","DOIUrl":"https://doi.org/10.1016/j.chembiol.2015.10.009","url":null,"abstract":"","PeriodicalId":9772,"journal":{"name":"Chemistry & biology","volume":"19 1","pages":"1577-87"},"PeriodicalIF":0.0,"publicationDate":"2015-12-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89636208","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
京公网安备 11010802042870号
京ICP备2023020795号-1
扫码关注我们
求助内容：
应助结果提醒方式：