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Small-Molecule Disruption of RAD52 Rings as a Mechanism for Precision Medicine in BRCA-Deficient Cancers. RAD52环的小分子破坏作为brca缺陷癌症的精准医疗机制。
Chemistry & biology
Pub Date : 2015-11-19 DOI: 10.1016/j.chembiol.2015.10.003
Gurushankar Chandramouly, Shane McDevitt, K. Sullivan, T. Kent, A. Luz, J. Glickman, M. Andrake, T. Skorski, R. Pomerantz
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引用次数: 76
Profiling of Free Fatty Acids Using Stable Isotope Tagging Uncovers a Role for Saturated Fatty Acids in Neuroexocytosis. 利用稳定同位素标记分析游离脂肪酸揭示了饱和脂肪酸在神经胞吐作用中的作用。
Chemistry & biology
Pub Date : 2015-11-19 DOI: 10.1016/j.chembiol.2015.09.010
V. Narayana, Vanesa M Tomatis, Tong Wang, D. Kvaskoff, F. Meunier
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引用次数: 26
Molecular Basis of Spectral Diversity in Near-Infrared Phytochrome-Based Fluorescent Proteins. 基于近红外植物色素的荧光蛋白光谱多样性的分子基础。
Chemistry & biology
Pub Date : 2015-11-19 DOI: 10.1016/j.chembiol.2015.10.007
Daria M Shcherbakova, Mikhail Baloban, Sergei Pletnev, Vladimir N Malashkevich, Hui Xiao, Zbigniew Dauter, Vladislav V Verkhusha

Near-infrared fluorescent proteins (NIR FPs) engineered from bacterial phytochromes (BphPs) are the probes of choice for deep-tissue imaging. Detection of several processes requires spectrally distinct NIR FPs. We developed an NIR FP, BphP1-FP, which has the most blue-shifted spectra and the highest fluorescence quantum yield among BphP-derived FPs. We found that these properties result from the binding of the biliverdin chromophore to a cysteine residue in the GAF domain, unlike natural BphPs and other BphP-based FPs. To elucidate the molecular basis of the spectral shift, we applied biochemical, structural and mass spectrometry analyses and revealed the formation of unique chromophore species. Mutagenesis of NIR FPs of different origins indicated that the mechanism of the spectral shift is general and can be used to design multicolor NIR FPs from other BphPs. We applied pairs of spectrally distinct point cysteine mutants to multicolor cell labeling and demonstrated that they perform well in model deep-tissue imaging.

从细菌噬色体（BphPs）中提取的近红外荧光蛋白（NIR FPs）是深层组织成像的首选探针。检测多个过程需要光谱不同的近红外荧光蛋白。我们开发了一种近红外荧光元件 BphP1-FP，它在 BphP 衍生的荧光元件中具有最蓝移的光谱和最高的荧光量子产率。我们发现，与天然 BphPs 和其他基于 BphP 的 FP 不同，这些特性源于胆绿素发色团与 GAF 结构域中的半胱氨酸残基的结合。为了阐明光谱偏移的分子基础，我们应用了生化、结构和质谱分析，结果发现形成了独特的发色团种类。对不同来源的近红外荧光蛋白进行突变分析表明，光谱偏移的机制是普遍的，可用于设计来自其他 BphPs 的多色近红外荧光蛋白。我们将一对光谱不同的点半胱氨酸突变体用于多色细胞标记，并证明它们在模型深层组织成像中表现良好。
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引用次数: 0
Probing the Substrate Specificity and Protein-Protein Interactions of the E. coli Fatty Acid Dehydratase, FabA. 探讨大肠杆菌脂肪酸脱水酶FabA的底物特异性和蛋白-蛋白相互作用。
Chemistry & biology
Pub Date : 2015-11-19 DOI: 10.1016/j.chembiol.2015.09.009
Kara L Finzel, C. Nguyen, D. R. Jackson, Aarushi Gupta, S. Tsai, M. Burkart
{"title":"Probing the Substrate Specificity and Protein-Protein Interactions of the E. coli Fatty Acid Dehydratase, FabA.","authors":"Kara L Finzel, C. Nguyen, D. R. Jackson, Aarushi Gupta, S. Tsai, M. Burkart","doi":"10.1016/j.chembiol.2015.09.009","DOIUrl":"https://doi.org/10.1016/j.chembiol.2015.09.009","url":null,"abstract":"","PeriodicalId":9772,"journal":{"name":"Chemistry & biology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2015-11-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79579892","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 24
Dissecting How Mtb Makes Its Wall, Buffering Endosomal pH, and Discovery of Ribocil. 剖析结核分枝杆菌壁的形成、缓冲内体pH值和发现核糖酸。
Chemistry & biology
Pub Date : 2015-11-19 DOI: 10.1016/j.chembiol.2015.11.003
M. Kostić
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引用次数: 0
Combining Suppression of Stemness with Lineage-Specific Induction Leads to Conversion of Pluripotent Cells into Functional Neurons. 结合抑制干细胞与谱系特异性诱导导致多能细胞转化为功能性神经元。
Chemistry & biology
Pub Date : 2015-11-19 DOI: 10.1016/j.chembiol.2015.10.008
Debasish Halder, Gyeong-Eon Chang, Debojyoti De, Eunji Cheong, K. Kim, I. Shin
{"title":"Combining Suppression of Stemness with Lineage-Specific Induction Leads to Conversion of Pluripotent Cells into Functional Neurons.","authors":"Debasish Halder, Gyeong-Eon Chang, Debojyoti De, Eunji Cheong, K. Kim, I. Shin","doi":"10.1016/j.chembiol.2015.10.008","DOIUrl":"https://doi.org/10.1016/j.chembiol.2015.10.008","url":null,"abstract":"","PeriodicalId":9772,"journal":{"name":"Chemistry & biology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2015-11-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83082429","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 5
Development of a Clickable Probe for Profiling of Protein Glutathionylation in the Central Cellular Metabolism of E. coli and Drosophila. 用于大肠杆菌和果蝇中央细胞代谢中蛋白谷胱甘肽化的可点击探针的开发。
Chemistry & biology
Pub Date : 2015-11-19 DOI: 10.1016/j.chembiol.2015.09.012
S. Feng, Yuling Chen, Fan Yang, Lei Zhang, Yiyi Gong, Gulishana Adilijiang, Yan Gao, Haiteng Deng
{"title":"Development of a Clickable Probe for Profiling of Protein Glutathionylation in the Central Cellular Metabolism of E. coli and Drosophila.","authors":"S. Feng, Yuling Chen, Fan Yang, Lei Zhang, Yiyi Gong, Gulishana Adilijiang, Yan Gao, Haiteng Deng","doi":"10.1016/j.chembiol.2015.09.012","DOIUrl":"https://doi.org/10.1016/j.chembiol.2015.09.012","url":null,"abstract":"","PeriodicalId":9772,"journal":{"name":"Chemistry & biology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2015-11-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85477661","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 14
Quantitative Lipoproteomics in Clostridium difficile Reveals a Role for Lipoproteins in Sporulation. 艰难梭菌定量脂蛋白组学揭示脂蛋白在产孢过程中的作用。
Chemistry & biology
Pub Date : 2015-11-19 DOI: 10.1016/j.chembiol.2015.10.006
Thomas M. Charlton, A. Kovács-Simon, S. L. Michell, N. Fairweather, E. Tate
{"title":"Quantitative Lipoproteomics in Clostridium difficile Reveals a Role for Lipoproteins in Sporulation.","authors":"Thomas M. Charlton, A. Kovács-Simon, S. L. Michell, N. Fairweather, E. Tate","doi":"10.1016/j.chembiol.2015.10.006","DOIUrl":"https://doi.org/10.1016/j.chembiol.2015.10.006","url":null,"abstract":"","PeriodicalId":9772,"journal":{"name":"Chemistry & biology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2015-11-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86181969","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 32
Visualization of Compartmentalized Kinase Activity Dynamics Using Adaptable BimKARs. 利用适应性BimKARs可视化区隔化激酶活性动态。
Chemistry & biology
Pub Date : 2015-11-19 DOI: 10.1016/j.chembiol.2015.10.004
Charlene Depry, Sohum Mehta, Ruojing Li, Jin Zhang
{"title":"Visualization of Compartmentalized Kinase Activity Dynamics Using Adaptable BimKARs.","authors":"Charlene Depry, Sohum Mehta, Ruojing Li, Jin Zhang","doi":"10.1016/j.chembiol.2015.10.004","DOIUrl":"https://doi.org/10.1016/j.chembiol.2015.10.004","url":null,"abstract":"","PeriodicalId":9772,"journal":{"name":"Chemistry & biology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2015-11-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86040848","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 20
Substrate Flexibility of a Mutated Acyltransferase Domain and Implications for Polyketide Biosynthesis. 突变酰基转移酶结构域的底物柔韧性及其对聚酮生物合成的影响。
Chemistry & biology
Pub Date : 2015-11-19 DOI: 10.1016/j.chembiol.2015.02.008
Kenny Bravo-Rodriguez, Stephan Klopries, K. R. Koopmans, U. Sundermann, S. Yahiaoui, Julia Arens, S. Kushnir, F. Schulz, E. Sánchez-García
{"title":"Substrate Flexibility of a Mutated Acyltransferase Domain and Implications for Polyketide Biosynthesis.","authors":"Kenny Bravo-Rodriguez, Stephan Klopries, K. R. Koopmans, U. Sundermann, S. Yahiaoui, Julia Arens, S. Kushnir, F. Schulz, E. Sánchez-García","doi":"10.1016/j.chembiol.2015.02.008","DOIUrl":"https://doi.org/10.1016/j.chembiol.2015.02.008","url":null,"abstract":"","PeriodicalId":9772,"journal":{"name":"Chemistry & biology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2015-11-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75141041","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 30
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