中华消化杂志最新文献

{"title":"Comparison of postoperative complications rates of endoscopic submucosal dissection in patients of different age groups with lesions at different locations","authors":"Weiqing Chen, Ming Hu, F. Ji","doi":"10.3760/CMA.J.ISSN.0254-1432.2019.07.007","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.0254-1432.2019.07.007","url":null,"abstract":"Objective \u0000To retrospectively analyze the characteristics of postoperative complications of endoscopic submucosal dissection (ESD) in patients of different age groups with lesions at different locations. \u0000 \u0000 \u0000Methods \u0000From January 2011 to October 2017, at The First Affiliated Hospital of Zhejiang University, clinical data of 1 688 patients who received ESD treatment (1 799 ESD operations) were collected. The lesions were located in the esophagus (n=232), stomach (n=1 225), duodenum (n=9), colon (n=152) and rectum (n=181). There were 500 patients (508 ESD operations) with underlying diseases, and 1 188 patients without underlying diseases. Most patients with lesions at stomach, esophagus and colon rectum, and these patients were divided into four age groups <45 years (n=202), 4559 years (n=643), 6074 years (n=744), and ≥75 years (n=99). The postoperative complications rates of ESD in different lesions among four age groups were compared. Chi-square test was performed for statistical analysis. \u0000 \u0000 \u0000Results \u0000From 2011 to 2017, the complication rates after ESD were 20.0% (8/40), 18.3% (21/115), 15.9% (35/220), 10.7% (31/291), 11.3% (40/355), 10.7% (41/384) and 10.7% (42/394), respectively, and the difference was not statistically significant (χ2=10.432, P=0.064). Further analysis revealed that the complication rates after ESD between 2011 and 2013 was significantly higher than that between 2014 and 2017 (17.1%, 64/375 vs. 10.8%, 154/1 424); and the difference was statistically significant (χ2=10.895, P<0.01). The complication rates of patients with lesions in the esophagus of<45 years group, 4559 years group, 6074 years group and ≥75 years group were 4.5% (1/22), 23.6% (17/72), 24.4% (31/127) and five cases (totally 11 cases). The complication rates of patients with lesions in the stomach of<45 years group, 4559 years group, 6074 years group and ≥75 years group were 19.2% (28/146), 14.2% (68/479), 15.8% (85/539) and 16.4% (10/61). The complication rates of lesions in the colon of<45 years group, 4559 years group, 6074 years group and ≥75 years group were one case (totally 12 cases), 3.4% (2/58), 10.9% (7/64) and five cases (totally 18 cases), respectively.The complication rates of lesions in the rectum of <45 years group, 4559 years group, 6074 years group and ≥75 years group were 3.3% (1/30), 10.4% (7/67), 19.4% (14/72) and two cases (totally 12 cases), respectively. There were significant differences in complication rates between different age groups with lesions in the esophagus (χ2=10.894, P=0.012) and the colon (χ2=13.540, P=0.004). In addition, the complication rates of patients with underlying diseases and without underlying diseases were 14.6% (74/508) and 12.5% (149/1 188), respectively, and there was no statistically significant difference (χ2=1.278, P=0.258). \u0000 \u0000 \u0000Conclusions \u0000The postoperative complications of ESD is closely associated with lesion location and age. For elderly patients, especially those with lesions in the esophagus and c","PeriodicalId":10009,"journal":{"name":"中华消化杂志","volume":"11 1","pages":"473-477"},"PeriodicalIF":0.0,"publicationDate":"2019-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73396746","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mechanism of microRNA-182 targeting and regulating forkhead box transcription factor 2 to promote the invasion, metastasis and angiogenesis of hepatocellular carcinoma","authors":"Yun-jian Wang, Min Zhang, Guohua You, Luyang Zhang","doi":"10.3760/CMA.J.ISSN.0254-1432.2019.07.006","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.0254-1432.2019.07.006","url":null,"abstract":"Objective \u0000To investigate the expression of miRNA-182 in hepatocellular carcinoma (HCC) cell lines and tissues, to verify the targeted regulation relationship between miRNA-182 and forkhead box transcription factor 2 (FOXF2) and to explore whether miRNA-182 can promote the invasion, metastasis and angiogenesis of HCC by targeting FOXF2. \u0000 \u0000 \u0000Methods \u0000From January to October 2017, a total of 41 patients with primary HCC admitting to Cancer Hospital Affiliated to Zhengzhou University and receiving operation were enrolled. The expression of miRNA-182 in tumor tissues and normal paracancerous tissues was detected by quantitative real-time fluorescence polymerase chain reaction (qRT-PCR). The effects of overexpression of miRNR-182 and FOXF2 on invasion and metastasis of HCC were detected by Transwell transfer experiment and the Boyden invasion experiment. The effects of miRNA-182 and FOXF2 on angiogenesis of HCC were determinded by in vitro angiogenesis experiments. The targeted regulation relationship between miRNA-182 and FOXF2 was investigated by dual luciferase reporter gene. The expression of FOXF2 at mRNA and protein level after miRNA-182 and miRNA-182 scramble transfection was detected by qRT-PCR and Western blotting method. T test was performed for statistical analysis. \u0000 \u0000 \u0000Results \u0000The expression in miRNA-182 in HCC tissue was higher than that in normal tissues adjacent to cancer (5.41±1.72 vs. 1.80±0.76), and the difference was statistically significant (t=-5.764, P<0.01). The results of Transwell transfer experiment and the Boyden invasion experiment showed that the number of cells passing through the membrane of the small chamber of miRNA-182 over-expression group was higher than that of miRNA-182 scramble group (85.65±4.86 vs. 31.43±2.41, 55.34±5.66 vs. 19.13±2.12), the number of cells passing through the membrane of the small chamber of miRNA-182+ FOXF2 over expression group was less than that of simple miRNA-182 over expression group (11.21±3.16 vs. 31.43±2.41, 8.67±2.83 vs. 19.13±2.12), and the differences were statistically significant (t=15.110, 9.220, 5.443 and 4.410; all P<0.05). The activity of luciferase of wild FOXF2 and miRNA-182 cotransfection group was lower than that of miRNA-182 scramble group (0.43±0.10 vs. 0.97±0.05), and the difference was statistically significant (t=8.042, P=0.012). However the activity of luciferase of mutated FOXF2 and miRNA-182 cotransfection group was higher than that of miRNA-182 scramble group (0.97±0.47 vs. 1.06±0.52), and the difference was not statistically significant (t=0.934, P=0.402). The results of in vitro angiogenesis experiments demonstrated that the number of constituent vessels in miRNA-182 over expression group was higher than that in miRNA-182 scramble group (14.27±1.77 vs. 5.91±1.42), and after FOXF2 over-expressing plasmids cotransfected, the angiogenesis ability restored (1.78±0.71 vs. 14.27±1.77), and the differences were statistically significant (t=6.530 and 4.570, both P<0.","PeriodicalId":10009,"journal":{"name":"中华消化杂志","volume":"5 1","pages":"466-472"},"PeriodicalIF":0.0,"publicationDate":"2019-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88888355","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Role and mechanism of circular RNA-vimentin in the proliferation and apoptosis of colorectal cancer cells","authors":"Jianning Yao, Xuexiu Zhang, Yan-zhen Zhang, Yanle Li, Chunfeng Wang","doi":"10.3760/CMA.J.ISSN.0254-1432.2019.07.005","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.0254-1432.2019.07.005","url":null,"abstract":"Objective \u0000To investigate the role and mechanism of circular RNA-vimentin (circ-VIM) in the proliferation and apoptosis of colorectal cancer cells. \u0000 \u0000 \u0000Methods \u0000From December 2016 to December 2017, at Department of General Surgery of The First Affiliated Hospital of Zhengzhou University, the clinical data of 100 patients who underwent radical resection of colorectal cancer and were confirmed by pathological examination after operation were collected. The tumor tissues and corresponding paracancerous tissues (negative control) were also collected. The expression of circ-VIM in the colorectal cancer tissues was determined by quantitative real-time polymerase chain reaction (qRT-PCR). The proliferation of HCT-116 and HT29 colorectal cancer cells was detected by cell counting kit-8 assay. The ratio of apoptosis of HCT-116 and HT29 cells was measured by annexin Ⅴ/propidium iodide double staining assay. The mitochondrial membrane potential of HCT-116 and HT29 cells was examined by 5, 5′, 6, 6′-tetrachloro-1, 1′, 3, 3′-tetraethyl-imidacarbocyanine iodide (JC-1) assay. The expression changes of protein kinase B and mammalian target of rapamycin were tested by Western blotting. The target miRNA of circ-VIM was predicted by miRDB software. T-test and chi-square test were performed for statistical analysis. \u0000 \u0000 \u0000Results \u0000The expression of circ-VIM in colorectal cancer tissues was 2.387±0.536, which was higher than that in corresponding paracancerous tissues (1.110±0.134), and the difference was statistically significant (t=23.096, P<0.01). And the expression levels of circ-VIM were significantly different in patients with different tumor size, TNM stage and lymph node metastasis (all P<0.05). The proliferation of HCT-116 cells and HT29 cells in lenti-circ-VIM group was 0.737±0.023 and 0.835±0.025, respectively, which were both higher than those in control group (0.449±0.020 and 0.531±0.019), and the differences were statistically significant (t=20.706 and -15.374, both P<0.01). The proliferation of HCT-116 cells and HT29 cells in lenti-circ-VIM-shRNA group was 0.236±0.027 and 0.243±0.019, which were lower than those in control group, and the differences were statistically significant (t=24.557 and -23.197, both P<0.01). The ratio of apoptosis of HCT-116 cells and HT29 cells in lenti-circ-VIM-shRNA group was (18.00±1.82)% and (20.80±0.61)%, which was higher than those in control group ((6.64±2.01)% and (7.35±1.36)%), and the differences were statistically significant (t=8.826 and 17.454, both P<0.01). The fluorescence intensity ratio of JC-1 aggregate and JC-1 monomer of HCT-116 cells and HT29 cells in lenti-circ-VIM-shRNA group was 2.21±0.12 and 1.40±0.11, which was lower than those in control group (14.54±1.00 and 9.24±1.18), and the differences were statistically significant (t=-19.558 and -15.685, both P<0.01), which indicated mitochondrial membrane potential decreased. After treated with lenti-circ-VIM-shRNA, the expression of phosphorylated protein ki","PeriodicalId":10009,"journal":{"name":"中华消化杂志","volume":"2016 1","pages":"458-465"},"PeriodicalIF":0.0,"publicationDate":"2019-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86650322","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Distribution of food specific antibodies in patients with inflammatory bowel disease","authors":"Yulin Yang, Chang Liu, Zhaoqing Yang, Hui Tao","doi":"10.3760/CMA.J.ISSN.0254-1432.2019.07.003","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.0254-1432.2019.07.003","url":null,"abstract":"Objective \u0000To investigate the incidence of food intolerance in patients with inflammatory bowel disease (IBD) and to analyze the differential diagnostic value of intolerant food in Crohn′s disease (CD) and ulcerative colitis (UC) and its effects on the diseases. \u0000 \u0000 \u0000Methods \u0000From January 2017 to June 2018, at Department of Gastroenterology and Hepatology, Jinling Hospital, Medical School of Nanjing University/General Hospital of Eastern Theater Command, PLA, a total of 252 IBD (154 CD and 98 UC) patients were enrolled. In the same period 46 non-IBD patients were recruited. Allergic diseases were excluded. The food-specific IgG antibodies were detected by enzyme linked immunosorbent assay (ELISA) in all enrolled patients. The chi-square test, Kruskal-Wallis test and Spearman correlation analysis were performed for statistical analysis. Regression analysis was used to screen the risk factors. \u0000 \u0000 \u0000Results \u0000The total positive rates of serum IgG antibody of corn, rice, soybean, tomato and wheat in CD patients were 60.4% (93/154), 57.8% (89/154), 42.9% (66/154), 68.2% (105/154) and 19.5% (30/154), respectively, which were higher than those in patients with UC (7.1%, 7/98; 5.1%, 5/98; 5.1%, 5/98; 16.3%, 16/98 and 3.1%, 3/98, respectively) and those of non-IBD patients (2.2%, 1/46; 2.2%, 1/46; 0, 0/46; 0, 0/46 and 0, 0/46, respectively), and the differences were statistically significant (χ2=70.940, 71.092, 42.185, 64.517, 14.187; 48.190, 44.270, 29.424, 66.029, 10.542; all P 0.05) and there was a negative correlation between the total number of IgG positive food and age in UC patients (rs=-0.376, P<0.01). The median number of total IgG positive food of patients with lesions involving the terminal ileum (L1) and ileocolon (L3) was two and four, respectively, and the difference was statistically significant(the statistic was 11.717, P=0.002). The median number of total IgG positive food of UC patients with rectal lesions (E1) and extensive colon lesions (E3) was zero and one, respectively, and the difference was statistically significant (the statistic was 7.191, P=0.022). In addition, positive IgG shrimp and soybean were risk factors of CD patients combined with extra-intestinal manifestations and low body mass index (odd ratio (OR)=24.558, 95%CI 2.243 to 268.936; OR=2.253, 95%CI 1.048 to 4.841; both P<0.05, respectively). \u0000 \u0000 \u0000Conclusions \u0000CD patients are more susceptible to food intolerance. The number of intolerant foods have differential diagnostic value in CD, UC and non-IBD. The larger the lesion of IBD patients, the more common the food intolerance. IgG antibody positive food positive, may affect extra-intestinal manifestations and nutritional status of CD patients. \u0000 \u0000 \u0000Key words: \u0000Inflammatory bowel diseases; Crohn disease; Colitis, ulcerative; Food antibodies","PeriodicalId":10009,"journal":{"name":"中华消化杂志","volume":"41 1","pages":"444-451"},"PeriodicalIF":0.0,"publicationDate":"2019-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85237359","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Magnetically controlled capsule gastroscopy: a new era of gastrointestinal endoscopy","authors":"Z. Liao","doi":"10.3760/CMA.J.ISSN.0254-1432.2019.06.002","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.0254-1432.2019.06.002","url":null,"abstract":"磁控胶囊胃镜诊断准确性高，与电子胃镜高度一致，已在国内外千余家单位应用50余万例，成为被广泛接受的舒适化胃镜检查新方法。与此同时，针对胶囊胃镜的循证医学研究也得到了全面开展。从应用的安全有效性到对胃癌的诊断效能评价，从检查技术的优化到胃肠道准备方案，不断的探索研究必将促使磁控胶囊胃镜的革新与突破，从而更好地为人类服务。","PeriodicalId":10009,"journal":{"name":"中华消化杂志","volume":"51 1","pages":"363-366"},"PeriodicalIF":0.0,"publicationDate":"2019-06-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91090753","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Analysis of risk factors for non-curative resection after endoscopic submucosal dissection in early esophageal cancer and high-grade intraepithelial neoplasia","authors":"Jiajia Yang, Yini Dang, Lei Peng, Duochen Jin, Huaiming Sang, Meihong Chen","doi":"10.3760/CMA.J.ISSN.0254-1432.2019.06.007","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.0254-1432.2019.06.007","url":null,"abstract":"Objective \u0000To investigate the risk factors for non-curative resection after endoscopic submucosal dissection (ESD) for early esophageal cancer and high-grade intraepithelial neoplasia. \u0000 \u0000 \u0000Methods \u0000The clinicopathological data of 427 cases of early esophageal cancer and high-grade intraepithelial neoplasia who underwent ESD was performed from January 2013 to December 2016 in the Department of Gastroenterology, First Affiliated Hospital of Nanjing Medical University were retrospectively analyzed. According to the results of postoperative pathology and immunohistochemistry, 339 patients were defined as curative resection group and 88 patients were defined as non-curative resection group. Chi-square test, univariate analysis and multivariate logistic regression analysis were used for statistical analysis. \u0000 \u0000 \u0000Results \u0000A total of 427 patients were enrolled in this study, with an average age of (63.2±7.7) years, including 96 cases of early esophageal cancer and 331 cases of high-grade intraepithelial neoplasia. The enbloc resection rate of ESD was 94.8%(405/427), 88 of them were non-curative resected, and the non-curative resection rate was 20.6%. Univariate analysis showed that early esophageal cancer (odds ratio (OR)=3.682, 95% confidence interval (CI) 2.216 to 6.118, P<0.01), submucosal infiltration (OR=10.220, 95%CI4.861 to 21.481, P<0.01), ESD indications (OR=6.005, 95%CI3.545 to 10.172, P<0.01) and lifting sign after injecting at the base of lesions (OR=2.508, 95%CI1.005 to 6.255, P=0.042) were statistically significant between non-curative resection group and curative resection group. Multivariate unconditional logistic regression analysis revealed that submucosal infiltration (OR=4.329, 95%CI1.758 to 10.661, P=0.001), not absolute indications of ESD (OR=6.484, 95%CI2.205 to 19.068, P=0.001) and negative lifting sign (OR=3.182, 95%CI1.171 to 8.651, P=0.023) were independent risk factors for non-curative resection. \u0000 \u0000 \u0000Conclusions \u0000Patients with early esophageal cancer, submucosal infiltration, not absolute indications for ESD and negative lifting signs are prone to non-curative resection. Moreover, submucosal infiltration, not absolute indications for ESD, and negative lifting signs are the independent risk factors for non-curative resection. \u0000 \u0000 \u0000Key words: \u0000Endoscopic submucosal dissection; Early esophageal cancer; Precancerous lesions; Non-curative resection","PeriodicalId":10009,"journal":{"name":"中华消化杂志","volume":"1 1","pages":"379-383"},"PeriodicalIF":0.0,"publicationDate":"2019-06-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89748394","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Influencing factors of different bowel preparation regimens on the quality of colonoscopy","authors":"Xiaohong Jiang, Weiqun Ding, Zhongguang Luo, Xinhua Huang","doi":"10.3760/CMA.J.ISSN.0254-1432.2019.06.008","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.0254-1432.2019.06.008","url":null,"abstract":"Objective \u0000To explore the influencing factors of different bowel preparation before colonoscopy on the intestinal cleanliness and polyp detection rate. \u0000 \u0000 \u0000Methods \u0000From March to August in 2018, at the Center of Endoscopy of Huashan Hospital Affiliated to Fudan University, the patients who underwent colonoscopy were selected and their general data of bowel preparation regimens were collected. Self-factors of the patients, different bowel preparation regimens, bowel preparation quality and polyp detection rate were observed. The quality of bowel preparation was evaluated by Boston bowel preparation scale (BBPS). T test, and analysis of variance and chi-square test were used for statistical analysis. \u0000 \u0000 \u0000Results \u0000Among 1 008 patients who underwent colonoscopy, there were 506 males and 502 females, and average age was (57.3±13.7) years. There were statistically significant differences in BBPS score of patients with different body mass index (BMI), Parkinson disease and history of abdominal surgery (F=3.319, t=-2.060 and -2.544; all P<0.05). The BBPS score of patients with three-day low residue diet before examination was higher than that of those without preparation before examination (6.04±2.50 vs. 5.54±2.73), and the difference was statistically significant (t=2.514, P=0.010). The BBPS scores of 2 000 mL polyethylene glycol electrolyte lavage solution (PEG) taken once, 2 000 mL PEG taken separately, 3 000 mL PEG taken once and 3 000 mL PEG taken separately were 5.06±2.88, 6.11±2.44, 5.94±2.32 and 6.10±2.47, respectively, and the difference was statistically significant (F=7.242, P<0.01). There were significant differences in polyp detection rates among the patients with different age, gender, BMI, and with history of constipation, hypertension and diabetes mellitus (χ2=33.170, 8.489, 12.024, 4.034, 26.790, 10.381; all P<0.05). The polyp detection rate of patients with oral methyl silicone oil was higher than that of patients without oral methyl silicone oil (52.6%, 30/57 vs. 29.7%, 221/744), and the difference was statistically significant (χ2=12.934, P<0.01). Age (odds ratio (OR)=1.328, 95% confidence interval (CI) 1.162 to 1.517) and BMI (OR=1.412, 95%CI1.115 to 1.787) were independent risk factors for polyp detection rate. \u0000 \u0000 \u0000Conclusions \u0000Parkinson disease, history of abdominal surgery and BMI are the related factors affecting the quality of bowel preparation before colonoscopy. Age and BMI are independent risk factors for polyp detection rate. \u0000 \u0000 \u0000Key words: \u0000Colonoscopes; Polyethylene glycols; Bowel preparation; Simethicone","PeriodicalId":10009,"journal":{"name":"中华消化杂志","volume":"32 1","pages":"384-389"},"PeriodicalIF":0.0,"publicationDate":"2019-06-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84684768","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Role and mechanism of microRNA-92b-3p in esophageal squamous cell carcinoma analyzed by weighted gene co-expression network analysis","authors":"Wanpeng Wang, C. Fu, Qidi Zhang, Chengshi Wang, Zhongxiang He, Yun Gu, Yanyan Zhang, Weijun Deng, J. Pu","doi":"10.3760/CMA.J.ISSN.0254-1432.2019.06.009","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.0254-1432.2019.06.009","url":null,"abstract":"Objective \u0000To screen the critical genes related to the development of esophageal squamous cell carcinoma (ESCC) by weighted gene co-expression network analysis (WGCNA) and to verify by experiments. \u0000 \u0000 \u0000Methods \u0000Gene expression data of ESCC were downloaded from gene expression omnibus (GEO) database based on gene chip platform (GPL) 570, GPL571, GPL96/97 or GPL14613 platform, respectively. Meanwhile, the obtained differentially expressed genes together with gene expression data of 81 ESCC patients from the cancer genome atlas (TCGA) and clinical data were analyzed by WGCNA to set up co-expression networks including mRNA and microRNA (miRNA). The expression of miRNA in ESCC tissues and paracancerous tissues was examined by quantitative real-time polymerase chain reaction (RT-PCR). And the expression of target protein Kruppel like factor 4 (KLF4) and desmocollin 2 (DSC2) were detected by immunohistochemistry. After ESCC cell line ECA-109 cells were transfected with miRNA-92b-3p mimic, cell cycle was tested by flow cytometry, the cell invasion and migration ability was measured by Transwell chamber assay and scratch-wound assay. The expression of KLF4 and DSC2 was observed by confocal laser scanning microscopy and Western blotting.The target genes were verified by luciferase assay. T-test, rank sum test, chi-square test and Pearson correlation analysis were performed for statistical analysis. \u0000 \u0000 \u0000Results \u0000A total of 4 023 differential expression gene (DEG) and 328 differential expression miRNA (DEM) were screened and 11 gene modules were set up by WGCNA. Among them, the brown modules were negatively associated with tumor grade and T stage (r=-0.340 and -0.268, P=0.002 and 0.016). Meanwhile, has-miR-92b and the potential target genes KLF4 and DSC2 were all in the brown module. Furthermore, the results of RT-PCR showed the expression of miRNA-92b-3p in ESCC tissues was higher than that in paracancerous tissues (3.052(1.652, 5.371) vs. 0.985(0.558, 2.032)), and the difference was statistically significant (Z=-4.021, P<0.01). The results of immunohistochemistry demonstrated that the positive rates of KLF4 and DSC2 in ESCC tissues were 43.3%(13/30) and 20.0%(6/30), respectively, which were lower than those of paracancerous tissues (70.0%(21/30) and 63.3%(19/30)), and the differences were statistically significant (χ2=4.344 and 1.589, both P<0.05). After ECA-109 cells were transfected with miRNA-92b-3p mimics, the percentage of cells at G0/G1 phase decreased ((63.71±2.83)% vs. (54.62±4.00)%) and the percentage of cells at the S phase and G2/M phase increased ((31.81±2.88)% vs. (41.20%±2.87)%, and (3.87±1.75)% vs. (8.10±1.71)%, respectively), and the differences were statistically significant (t=3.215, 4.000 and 2.998; P=0.032, 0.016 and 0.040). The invasion and migration ability of the cells were significantly improved (79.67±27.54 vs. 280.33±46.18, (69.72±3.91)% vs.(84.90±5.25)%), and the differences were statistically significant (t=6.465 and 4.019, P=","PeriodicalId":10009,"journal":{"name":"中华消化杂志","volume":"116 1","pages":"390-396"},"PeriodicalIF":0.0,"publicationDate":"2019-06-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87830460","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Progress in diagnosis and treatment of enteroscopy","authors":"Fachao Zhi","doi":"10.3760/CMA.J.ISSN.0254-1432.2019.06.006","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.0254-1432.2019.06.006","url":null,"abstract":"目前临床应用的小肠镜主要是气囊辅助小肠镜，包括双气囊小肠镜和单气囊小肠镜。其对于小肠疾病的诊断与随访已经比较普及，如小肠出血、不明原因腹泻和腹痛、反复发作的不全肠梗阻、不明原因贫血等；对某些小肠疾病的治疗也正逐渐开展，如小肠异物取出术、小肠息肉内镜下切除术、小肠出血内镜下止血术、小肠狭窄内镜下扩张术及支架置入术、特殊解剖状态下的内镜逆行胰胆管造影术等。诊治兼具将是小肠镜的应用常规。","PeriodicalId":10009,"journal":{"name":"中华消化杂志","volume":"29 1","pages":"376-378"},"PeriodicalIF":0.0,"publicationDate":"2019-06-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83576450","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Serum metabolomics analysis and establishment of diagnostic model of pancreatic cancer associated diabetes","authors":"Xiangyi He, Yuan Fang, Baiyong Shen, Yao-zong Yuan","doi":"10.3760/CMA.J.ISSN.0254-1432.2019.06.010","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.0254-1432.2019.06.010","url":null,"abstract":"Objective \u0000To establish the diagnostic model based on detection of serum biomarkers in pancreatic cancer (PC) associated diabetes. \u0000 \u0000 \u0000Methods \u0000From June 2013 to July 2014, at Ruijin Hospital, School of Medicine, Shanghai Jiao Tong University, 30 patients diagnosed with PC companied with new onset diabetic mellitus and 30 patients with new onset type 2 diabetic mellitus, were enrolled. Serum samples were examined by liquid chromatography-mass spectrometry (LC-MS) for metabolomics analysis. Orthogonal partial least square (OPLS) was performed for raw data analysis to obtain the differentially expressed metabolites between two groups. The first 15 cases of each group were taken as training samples and the left as validation samples. The model was established using logistic regression via stepwise differentially expressed metabolites and clinical data input in training samples. The diagnostic efficiency of the model was verified in validating samples. \u0000 \u0000 \u0000Results \u0000Ten differentially expressed metabolites were identified in PC companied with new onset diabetic mellitus group and new onset type 2 diabetic mellitus group. The differentially expressed metabolites identified in positive ion mode were 3-ketosphingosine, arachidonoyl dopamine, phosphatidylethanolamine (18∶2), ubiquinone-1 and valine. The differentially expressed metabolites identified in negative ion mode were C16 sphingosine-1-phosphate, keto palmitic acid, isoleucine, N-succinyl-L-diaminopimelic acid and uridine. The diagnostic model was established in training samples: p=e(Xβ)/(1+ e(Xβ)), (Xβ)=-158.975-1.891 (age)+ 0.309 (phosphatidylethanolamine 18∶2)+ 1.035 (C16 sphingosine-1-phosphate)+ 0.084 (isoleucine)+ 1.114 5 (N-succinyl-L-diaminopimelic acid). The area under curve (AUC) of receiver operating characteristic (ROC) of this model was 0.982 in validation samples, the sensitivity and specificity were both 93.3%. \u0000 \u0000 \u0000Conclusion \u0000Serum metabolomics-based diagnostic approach is a promising method for screening PC from new onset diabetic mellitus. \u0000 \u0000 \u0000Key words: \u0000Pancreatic neoplasms; Diabetes mellitus; Serum metabolomics","PeriodicalId":10009,"journal":{"name":"中华消化杂志","volume":"226 1","pages":"397-401"},"PeriodicalIF":0.0,"publicationDate":"2019-06-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80127947","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}