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Interaction between renin–angiotensin and sympathetic nervous systems in a rat model of pressure overload cardiac hypertrophy 肾素-血管紧张素与交感神经系统在压力过载心肌肥厚大鼠模型中的相互作用
Pub Date : 2009-09-09 DOI: 10.1111/j.1474-8665.2009.00445.x
H. A. Rathore, A. S. Munavvar, N. A. Abdullah, A. H. Khan, B. Fathihah, M. H. NurJannah, N. A. Raisa, K. R. L. Anand Swarup, M. H. Abdullah, I. M. Salman, E. J. Johns

1 A raised cardiac workload activates neurohormones which will increase muscle mass and shift contractility to the right along the Frank-Starling curve.

2 This study examined the interaction between the SNS and RAS in contributing to vascular responsiveness following the development of cardiac hypertrophy due to aortic banding.

3 Sprague Dawley rats (180–200 g) were assigned to one of six groups; Normal, Sham-operated, Aortic Banded (AB), Aortic Banded treated with losartan (ABLOS), Aortic Banded treated with 6-hydroxydopamine (ABSYMP) and Aortic banded treated with both losartan and 6-hydroxydopamine (ABSYMPLOS). A constricting band was placed around the supra renal aorta on day zero with drug treatment from day 37 to day 44. Vasopressor responses to noradrenaline, phenylephrine, methoxamine and angiotensin II were measured on day 45.

4 The magnitudes of the MAP responses to all vasoactive agents, expressed as percentage changes, were similar in Normal and Sham groups, but reduced in the AB group. ABLOS group showed attenuated response to ANGII whereas all responses were enhanced in the ABSYM group.

5 A positive interaction between the two systems was observed with α1A-adrenoceptors identified as a major component of SNS and AT1 receptors of RAS to induce vasopressor effects.

心脏负荷增加会激活神经激素,从而增加肌肉量并使收缩力沿弗兰克-斯塔林曲线向右移动。2本研究探讨了SNS和RAS在主动脉束带导致心脏肥厚后血管反应性的相互作用。取Sprague Dawley大鼠3只(180 ~ 200 g),分为6组;正常、假手术、主动脉束带(AB)、氯沙坦治疗的主动脉束带(ABLOS)、6-羟多巴胺治疗的主动脉束带(ABSYMP)和氯沙坦和6-羟多巴胺治疗的主动脉束带(ABSYMPLOS)。第0天在肾上主动脉周围行缩窄带,第37天至第44天给予药物治疗。在第45天测量血管加压素对去甲肾上腺素、苯肾上腺素、甲氧胺和血管紧张素II的反应。4 . MAP对所有血管活性药物的反应大小(以百分比变化表示)在正常组和假手术组相似,但在AB组有所降低。ABLOS组对ANGII的反应减弱,而ABSYM组的所有反应均增强。5观察到两个系统之间的正相互作用,α 1a -肾上腺素受体被确定为SNS的主要成分和RAS的AT1受体诱导血管加压作用。
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引用次数: 12
Influences, decisions and serendipity: an autobiography 影响,决定和意外:一本自传
Pub Date : 2009-06-22 DOI: 10.1111/j.1474-8673.2009.00434.x
K. J. Broadley
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引用次数: 0
Nitrite reduction by the red cell membrane: a mechanism with a biological role? 红细胞亚硝酸盐还原:一种具有生物学作用的机制?
Pub Date : 2009-06-22 DOI: 10.1111/j.1474-8673.2009.00439.x
A. G. Pinder, S. C. Rogers, K. Morris, P. E. James

The possible role of nitric oxide (NO) metabolites in vivo has gained much interest in recent years, in particular, the interaction of these species with red blood cells. We investigated the potential for the membrane of red blood cells to act as a nitrite reductase site. Using both EPR (electromagnetic resonance spectroscopy) and ozone based chemiluminescence we were able to demonstrate NO generation from nitrite by the red cell membrane. The exact components responsible for this action are yet to be elucidated, but the response was unchanged by L-NMMA suggesting that eNOS is not involved. Reduction at the membrane could provide an entry route for NO into the red cell where it could produce potentially bioactive species e.g. nitrosylated proteins (RSNOs). If the nitrite reduction occurred on the outer surface of the red cell membrane it is also feasible that some NO may escape auto capture by that red cell, however in whole blood it is likely to be rapidly metabolised. In conclusion, this mechanism could provide a route by which nitrite, acting as a substrate, could be reduced to NO and form other, more biologically accessible species.

近年来,一氧化氮(NO)代谢物在体内的可能作用引起了人们的极大兴趣,特别是这些物质与红细胞的相互作用。我们研究了红细胞膜作为亚硝酸盐还原酶位点的潜力。使用EPR(电磁共振光谱)和基于臭氧的化学发光,我们能够证明亚硝酸盐通过红细胞膜生成NO。导致这一作用的确切成分尚未阐明,但L-NMMA的反应没有改变,这表明eNOS与此无关。膜上的还原可以为NO进入红细胞提供进入途径,在那里它可以产生潜在的生物活性物质,例如亚硝基化蛋白(RSNOs)。如果亚硝酸盐还原发生在红细胞的外表面,也有可能一些NO可能会逃脱红细胞的自动捕获,然而在全血中,它可能会被迅速代谢。综上所述,这一机制可能为亚硝酸盐作为底物还原为NO并形成其他更容易获得的物种提供了一条途径。
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引用次数: 0
Muscarinic receptor-mediated nitric oxide release in a K562 erythroleukaemia cell line 毒蕈碱受体介导的一氧化氮在K562红白血病细胞系中的释放
Pub Date : 2009-06-22 DOI: 10.1111/j.1474-8673.2009.00431.x
H. Cabadak, E. Küçükibrahimoğlu, B. Aydın, B. Kan, M. Zafer Gören

1 In the present study we have investigated the expression of muscarinic receptors in K562 erythroleukaemic cells and the effects of muscarinic agonist and antagonists on extracellular citrulline levels in these cells, as a marker of nitric oxide (NO) generation.

2 Muscarinic acetylcholine receptors (M1–M5) play key roles in regulating many diverse physiological processes. Recent studies suggest that muscarinic receptors mediate some cellular events in haematopoietic cells. Multiple subtypes of muscarinic receptors are expressed in different human cells. NO, a free radical and a signaling molecule, is involved in the regulation of many physiological functions and derived from certain nitric oxide synthases (NOS), which are related to muscarinic receptors.

3 In this study, the presence of M2, M3 and M4 subtypes in K562, an erythroleukaemic cell line, was demonstrated by using the reverse transcriptase-polymerase chain reaction. Moreover, the generation of NO induced by carbachol, a non-selective muscarinic agonist, was investigated by using high-performance liquid chromatography to measure changes in extracellular l-citrulline levels.

4 We found that carbachol enhanced l-citrulline production in K562 erythroleukaemic cells. The effect of carbachol on l-citrulline production was antagonized by atropine and 4-diphenylacetoxy-N-methylpiperidine (4-DAMP), while tropicamide had little effect. These results suggest that the muscarinic receptor M3 subtype may mediate NO signaling in K562 erythroleukaemic cells.

在本研究中,我们研究了毒蕈碱受体在K562红白血病细胞中的表达,以及毒蕈碱激动剂和拮抗剂对这些细胞外瓜氨酸水平的影响,瓜氨酸是一氧化氮(NO)生成的标志。毒蕈碱乙酰胆碱受体(M1-M5)在调节多种生理过程中起关键作用。最近的研究表明,毒蕈碱受体介导造血细胞的一些细胞事件。毒蕈碱受体的多种亚型在不同的人类细胞中表达。一氧化氮(NO)是一种自由基和信号分子,参与许多生理功能的调节,来源于某些与毒蕈碱受体有关的一氧化氮合酶(NOS)。3本研究通过逆转录-聚合酶链反应证实了红白血病细胞系K562中存在M2、M3和M4亚型。此外,采用高效液相色谱法测定细胞外l-瓜氨酸水平的变化,研究了非选择性毒菌碱激动剂carbachol诱导NO的生成。4 .我们发现,乙醇能增强K562红白血病细胞中l-瓜氨酸的生成。阿托品和4-二苯基乙酰氧基- n -甲基哌啶(4-DAMP)可拮抗卡巴醇对l-瓜氨酸生成的影响,而tropicamide对其影响不大。这些结果提示毒蕈碱受体M3亚型可能介导K562红白血病细胞NO信号转导。
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引用次数: 12
Mast cells, peptides and cardioprotection – an unlikely marriage? 肥大细胞、多肽和心脏保护——不可能的联姻?
Pub Date : 2009-06-22 DOI: 10.1111/j.1474-8673.2009.00436.x
S. K. Walsh, K. A. Kane, C. L. Wainwright

1 Mast cells have classically been regarded as the ‘bad guys’ in the setting of acute myocardial ischaemia, where their released contents are believed to contribute both to tissue injury and electrical disturbances resulting from ischaemia. Recent evidence suggests, however, that if mast cell degranulation occurs in advance of ischaemia onset, this may be cardioprotective by virtue of the depletion of mast cell contents that can no longer act as instruments of injury when the tissue becomes ischaemic.

2 Many peptides, such as ET-1, adrenomedullin, relaxin and atrial natriuretic peptide, have been demonstrated to be cardioprotective when given prior to the onset of myocardial ischaemia, although their physiological functions are varied and the mechanisms of their cardioprotective actions appear to be diverse and often ill defined. However, one common denominator that is emerging is the ability of these peptides to modulate mast cell degranulation, raising the possibility that peptide-induced mast cell degranulation or stabilization may hold the key to a common mechanism of their cardioprotection.

3 The aim of this review was to consolidate the evidence implying that mast cell degranulation could play both a detrimental and protective role in myocardial ischaemia, depending upon when it occurs, and that this may underlie the cardioprotective effects of a range of diverse peptides that exerts physiological effects within the cardiovascular system.

肥大细胞通常被认为是急性心肌缺血的“坏人”,其释放的内容物被认为是缺血导致组织损伤和电干扰的原因。然而,最近的证据表明,如果肥大细胞脱颗粒发生在缺血发作之前,这可能是由于肥大细胞内容物的消耗而具有心脏保护作用,当组织缺血时,肥大细胞内容物不再作为损伤工具。2许多多肽,如ET-1、肾上腺髓质素、松弛素和房利钠肽,已被证明在心肌缺血发作前给予心脏保护作用,尽管它们的生理功能各不相同,其心脏保护作用的机制似乎多种多样,而且往往不明确。然而,一个正在出现的共同点是这些肽调节肥大细胞脱颗粒的能力,提高了肽诱导肥大细胞脱颗粒或稳定的可能性,这可能是它们保护心脏的共同机制的关键。3本综述的目的是巩固肥大细胞脱颗粒可能在心肌缺血中发挥有害和保护作用的证据,这取决于它发生的时间,这可能是一系列不同肽在心血管系统中发挥生理作用的心脏保护作用的基础。
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引用次数: 12
Pharmacology of the internal anal sphincter and its relevance to faecal incontinence 内肛门括约肌的药理学及其与大便失禁的相关性
Pub Date : 2009-06-22 DOI: 10.1111/j.1474-8673.2009.00437.x
K. Mills, R. Chess-Williams

1 The internal anal sphincter (IAS) has a spontaneous tone and is the main contributor to the maintenance of faecal continence. The spontaneous resting tone exhibited by the sphincter can be modified by neurotransmitters from the autonomic and enteric nervous systems.

2 In this review, the influence of the sympathetic and parasympathetic nervous systems on IAS tone are discussed and the putative roles of nitric oxide, carbon monoxide, vasoactive intestinal peptide and adenosine triphosphate in non-adrenergic non-cholinergic transmission are considered.

3 Faecal incontinence is a common condition that places a heavy financial burden on the health service and severely affects patients’ quality of life. Resting anal pressure is reduced in patients with faecal incontinence and agents that increase sphincter tone tend to relieve symptoms. The results of clinical studies of the use of phenylephrine to treat faecal incontinence are reviewed.

4 It is concluded that the IAS is a potential target for drug development for the treatment of faecal incontinence.

1内肛门括约肌(IAS)具有自发性张力,是维持大便失禁的主要贡献者。自主神经系统和肠神经系统的神经递质可以改变括约肌自发的静息张力。本文讨论了交感和副交感神经系统对IAS张力的影响,并考虑了一氧化氮、一氧化碳、血管活性肠肽和三磷酸腺苷在非肾上腺素能、非胆碱能传递中的可能作用。大便失禁是一种常见的疾病,给卫生服务带来沉重的经济负担,并严重影响患者的生活质量。大便失禁患者静息时肛门压力降低,增加括约肌张力的药物往往能缓解症状。本文综述了应用苯肾上腺素治疗大便失禁的临床研究结果。因此,IAS是治疗大便失禁药物开发的潜在靶点。
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引用次数: 15
Time course of vascular prostanoid production in the fructose-hypertensive rat 果糖高血压大鼠血管中前列腺素生成的时间过程
Pub Date : 2009-06-22 DOI: 10.1111/j.1474-8673.2009.00433.x
A. M. Puyó, M. Zabalza, M. Mayer, A. Carranza, H. A. Peredo

1 There is a relationship between hypertension, insulin resistance and an altered plasmatic lipid profile known as ‘metabolic syndrome’. Fructose (F) overload induces in the rat a mild hypertension associated with metabolic alterations such as hyperglycemia, hypertriglyceridemia and insulin resistance, resembling such syndrome.

2 Prostanoids (PR), metabolites of arachidonic acid, include vasoactive substances synthesized and released by the vessel wall. An altered pattern of PR release has been previously found in mesenteric vessels of experimental diabetic rats.

3 This study analyzed the effects of F-overload during different periods (4, 9, 15 and 22 weeks) on PR release in aorta (A) and mesenteric vascular beds (MVB). Animals received tap water (control) or F solution (10% w/v) to drink.

4 Rats with F overload showed significantly higher systolic blood pressure, glycemia and triglyceridemia than controls; but no differences in this parameters were found among periods of treatment either in controls or experimental animals.

5 In A, prostacyclin was decreased at 9, 15 and 22 weeks of treatment when compared to 4 weeks and controls. In MVB, prostacyclin showed different patterns of release in the studied periods of F overload. Prostaglandin (PG) E2 diminish in MVB at the same extent in all periods. No changes were observed in A. The vasoconstrictor thromboxane was elevated in the MVB at 9 weeks. PGF2α, also a vasoconstrictor, remains unchanged.

6 In conclusion, F overload provokes in the rat a decrease in the vascular production of vasodilator PR and, in one of the studied periods, an increase in the release of the vasoconstrictor thromboxane, leading to a negative imbalance in the prostacylin/thromboxane ratio. This could be involved in the blood pressure alterations found in this experimental model of metabolic syndrome.

高血压、胰岛素抵抗和被称为“代谢综合征”的血浆脂质谱改变之间存在关系。果糖(F)超载在大鼠中引起轻度高血压,并伴有代谢改变,如高血糖、高甘油三酯血症和胰岛素抵抗,类似于此类综合征。前列腺素(PR)是花生四烯酸的代谢物,包括血管壁合成和释放的血管活性物质。先前在实验性糖尿病大鼠的肠系膜血管中发现了PR释放模式的改变。本研究分析了不同时期(4、9、15和22周)f -过载对主动脉(A)和肠系膜血管床(MVB) PR释放的影响。给动物喝自来水(对照组)或F溶液(10% w/v)。4 . F超载大鼠收缩压、血糖和甘油三酯血症明显高于对照组;但在对照组和实验动物的治疗期间,这些参数没有发现差异。5在A中,与4周和对照组相比,前列环素在治疗第9、15和22周时减少。在MVB中,前列环素在F过载的研究期间表现出不同的释放模式。前列腺素(PG) E2在各时期均有相同程度的减少。a组未见变化,9周时MVB血管收缩剂血栓素升高。PGF2α,也是一种血管收缩剂,保持不变。综上所述,F超载引起大鼠血管扩张剂PR的减少,在其中一个研究时期,血管收缩剂血栓素的释放增加,导致前列腺素/血栓素比例负失衡。这可能与代谢综合征实验模型中发现的血压变化有关。
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引用次数: 3
Purinergic regulation of vascular tone and remodelling 嘌呤能调节血管张力和血管重构
Pub Date : 2009-06-22 DOI: 10.1111/j.1474-8673.2009.00435.x
G. Burnstock

1 Purinergic signalling is involved both in short-term control of vascular tone and in longer-term control of cell proliferation, migration and death involved in vascular remodelling.

2 There is dual control of vascular tone by adenosine 5′-triphosphate (ATP) released from perivascular nerves and by ATP released from endothelial cells in response to changes in blood flow (shear stress) and hypoxia.

3 Both ATP and its breakdown product, adenosine, regulate smooth muscle and endothelial cell proliferation.

4 These regulatory mechanisms are important in pathological conditions, including hypertension, atherosclerosis, restenosis, diabetes and vascular pain.

嘌呤能信号既参与血管张力的短期控制,也参与血管重构过程中细胞增殖、迁移和死亡的长期控制。血管张力受血管周围神经释放的5 ' -三磷酸腺苷(ATP)和内皮细胞响应血流变化(剪切应力)和缺氧释放的ATP的双重控制。ATP及其分解产物腺苷调节平滑肌和内皮细胞的增殖。这些调节机制在病理条件下很重要,包括高血压、动脉粥样硬化、再狭窄、糖尿病和血管疼痛。
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引用次数: 103
The role of nitric oxide in A3 adenosine receptor-mediated cardioprotection 一氧化氮在A3腺苷受体介导的心脏保护中的作用
Pub Date : 2009-06-22 DOI: 10.1111/j.1474-8673.2009.00438.x
A. Hussain, P. Karjian, H. Maddock

1 Limiting the impact of ischemia reperfusion-related cell death is of vital importance given the enormous figures of heart related mortality in the world.

2 Coronary heart disease (CHD) is responsible for over 100 000 deaths in the UK each year, and is the most common cause of premature death in the UK and as a whole it is estimated that there are just over 1.5 million men, and 1.1 million women, who have suffered CHD in the form of either angina or myocardial infarction (http://www.heartstats.org).

3 In patients undergoing standard clinical reperfusion treatment today such as thrombolysis, percutaneous coronary angioplasty (primary PCTA), and bypass surgery, there remains an underscored need for novel therapies and strategies to reduce post-ischemic infarct size.

4 This review focuses on some of the intracellular signalling pathways that have been proposed to be coupled to A3 adenosine receptors in order to reduce post-ischemic infarct size, in particular the role of nitric oxide in A3 adenosine receptor-mediated cardioprotection is discussed.

1考虑到世界上心脏相关死亡率的巨大数字,限制缺血再灌注相关细胞死亡的影响至关重要。2冠心病(CHD)每年在英国造成超过10万人死亡,是英国过早死亡的最常见原因,总体而言,估计有超过150万男性和110万女性以心绞痛或心肌梗死的形式患有冠心病(http://www.heartstats.org)。在目前接受标准临床再灌注治疗的患者中,如溶栓、经皮冠状动脉成形术(原发性PCTA)和搭桥手术,仍然需要新的治疗方法和策略来减少缺血性梗死后的面积。这篇综述的重点是已经提出的一些细胞内信号通路与A3腺苷受体偶联,以减少缺血性梗死后的大小,特别是一氧化氮在A3腺苷受体介导的心脏保护中的作用被讨论。
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引用次数: 6
Adventitia removal does not modify the α1D-adrenoceptors response in aorta during hypertension and ageing 在高血压和衰老期间,去除外膜不会改变主动脉α 1d -肾上腺素受体的反应
Pub Date : 2009-06-22 DOI: 10.1111/j.1474-8673.2009.00432.x
J. H. Gómez-Zamudio, R. Villalobos-Molina

1 The aim of the current study was to characterize the α1-adrenergic receptors (α1-ARs) present in the isolated tunica media of aorta, in normotensive Wistar Kyoto (WKY) and spontaneously hypertensive (SHR) rats during the course of ageing and hypertension (rats of 1, 3, 6 and 12 months of age). In all vessels, endothelium was removed.

2 In isolated aortic rings, phenylephrine increased contraction in a concentration- and age-dependent manner and was impaired in old SHR compared with WKY rats.

3 The α1-AR selective antagonist prazosin showed high affinity (pA2) in vessels from both rat strains.

4 The potency of the α1A-AR selective antagonists, RS 100329 (5-methyl-3-[3-[4-[2-(2,2,2,-trifluoroethoxy) phenyl]-1-piperazinyl] propyl]-2,4-(1H)-pyrimidinedione) and 5-methylurapidil in antagonizing aortic phenylephrine-responses was low.

5 The α1D-AR selective antagonist, BMY 7378 (8-[2-[4-(2-methoxyphenyl)-1 piperazynil] ethyl]-8-azaspiro [4.5] decane-7,9-dione) potently blocked phenylephrine-induced responses in aorta from both strains and at all ages.

6 Adventitia removal decreased Emax in older rats and modified the relative affinity (pD2), but did not affect the affinity of the selective antagonists.

7 The results suggest that aorta tunica α1D-AR is the main subtype involved in phenylephrine-induced contraction of rat aorta, while α1A-AR plays only a minor role.

8 Ageing and hypertension did not modify α1-ARs in the blood vessel and the tunica adventitia does not seem to participate in contraction, even though α1-ARs are expressed.

1本研究的目的是表征α1-肾上腺素能受体(α1-ARs)在离体主动脉中膜、正常血压Wistar Kyoto (WKY)和自发性高血压(SHR)大鼠(1、3、6和12月龄大鼠)衰老和高血压过程中存在的特征。在所有血管中,内皮被去除。在分离的主动脉环中,苯肾上腺素以浓度和年龄依赖的方式增加收缩,与WKY大鼠相比,老年SHR大鼠的收缩功能受损。α1-AR选择性拮抗剂prazosin在两大鼠血管中均表现出高亲和力(pA2)。4 α1A-AR选择性拮抗剂RS 100329(5-甲基-3-[3-[4-[2-(2,2,2,-三氟乙氧基)苯基]-1-哌嗪基]丙基]-2,4-(1H)-嘧啶二酮)和5-甲基乌拉地尔拮抗主动脉苯肾上腺素反应的效价较低。α1D-AR选择性拮抗剂BMY 7378(8-[2-[4-(2-甲氧基苯基)-1哌嗪基]乙基]-8-阿兹匹斯罗[4.5]十二烷-7,9-二酮)在两种菌株和所有年龄的主动脉中都能有效阻断苯肾上腺素诱导的反应。6去除外膜可降低老年大鼠的Emax并改变其相对亲和力(pD2),但不影响选择性拮抗剂的亲和力。7结果提示,在肾上腺素诱导的大鼠主动脉收缩中,主动脉α1D-AR是主要亚型,α1A-AR仅起次要作用。8衰老和高血压并没有改变血管中的α1-ARs,即使α1-ARs表达,外膜似乎也不参与收缩。
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引用次数: 1
期刊
Autonomic and Autacoid Pharmacology
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