Pub Date : 2009-09-09DOI: 10.1111/j.1474-8665.2009.00445.x
H. A. Rathore, A. S. Munavvar, N. A. Abdullah, A. H. Khan, B. Fathihah, M. H. NurJannah, N. A. Raisa, K. R. L. Anand Swarup, M. H. Abdullah, I. M. Salman, E. J. Johns
1 A raised cardiac workload activates neurohormones which will increase muscle mass and shift contractility to the right along the Frank-Starling curve.
2 This study examined the interaction between the SNS and RAS in contributing to vascular responsiveness following the development of cardiac hypertrophy due to aortic banding.
3 Sprague Dawley rats (180–200 g) were assigned to one of six groups; Normal, Sham-operated, Aortic Banded (AB), Aortic Banded treated with losartan (ABLOS), Aortic Banded treated with 6-hydroxydopamine (ABSYMP) and Aortic banded treated with both losartan and 6-hydroxydopamine (ABSYMPLOS). A constricting band was placed around the supra renal aorta on day zero with drug treatment from day 37 to day 44. Vasopressor responses to noradrenaline, phenylephrine, methoxamine and angiotensin II were measured on day 45.
4 The magnitudes of the MAP responses to all vasoactive agents, expressed as percentage changes, were similar in Normal and Sham groups, but reduced in the AB group. ABLOS group showed attenuated response to ANGII whereas all responses were enhanced in the ABSYM group.
5 A positive interaction between the two systems was observed with α1A-adrenoceptors identified as a major component of SNS and AT1 receptors of RAS to induce vasopressor effects.
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Pub Date : 2009-06-22DOI: 10.1111/j.1474-8673.2009.00434.x
K. J. Broadley
{"title":"Influences, decisions and serendipity: an autobiography","authors":"K. J. Broadley","doi":"10.1111/j.1474-8673.2009.00434.x","DOIUrl":"10.1111/j.1474-8673.2009.00434.x","url":null,"abstract":"","PeriodicalId":100151,"journal":{"name":"Autonomic and Autacoid Pharmacology","volume":"29 3","pages":"51-62"},"PeriodicalIF":0.0,"publicationDate":"2009-06-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/j.1474-8673.2009.00434.x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"28276285","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2009-06-22DOI: 10.1111/j.1474-8673.2009.00439.x
A. G. Pinder, S. C. Rogers, K. Morris, P. E. James
The possible role of nitric oxide (NO) metabolites in vivo has gained much interest in recent years, in particular, the interaction of these species with red blood cells. We investigated the potential for the membrane of red blood cells to act as a nitrite reductase site. Using both EPR (electromagnetic resonance spectroscopy) and ozone based chemiluminescence we were able to demonstrate NO generation from nitrite by the red cell membrane. The exact components responsible for this action are yet to be elucidated, but the response was unchanged by L-NMMA suggesting that eNOS is not involved. Reduction at the membrane could provide an entry route for NO into the red cell where it could produce potentially bioactive species e.g. nitrosylated proteins (RSNOs). If the nitrite reduction occurred on the outer surface of the red cell membrane it is also feasible that some NO may escape auto capture by that red cell, however in whole blood it is likely to be rapidly metabolised. In conclusion, this mechanism could provide a route by which nitrite, acting as a substrate, could be reduced to NO and form other, more biologically accessible species.
{"title":"Nitrite reduction by the red cell membrane: a mechanism with a biological role?","authors":"A. G. Pinder, S. C. Rogers, K. Morris, P. E. James","doi":"10.1111/j.1474-8673.2009.00439.x","DOIUrl":"10.1111/j.1474-8673.2009.00439.x","url":null,"abstract":"<p>The possible role of nitric oxide (NO) metabolites <i>in vivo</i> has gained much interest in recent years, in particular, the interaction of these species with red blood cells. We investigated the potential for the membrane of red blood cells to act as a nitrite reductase site. Using both EPR (electromagnetic resonance spectroscopy) and ozone based chemiluminescence we were able to demonstrate NO generation from nitrite by the red cell membrane. The exact components responsible for this action are yet to be elucidated, but the response was unchanged by L-NMMA suggesting that eNOS is not involved. Reduction at the membrane could provide an entry route for NO into the red cell where it could produce potentially bioactive species e.g. nitrosylated proteins (RSNOs). If the nitrite reduction occurred on the outer surface of the red cell membrane it is also feasible that some NO may escape auto capture by that red cell, however in whole blood it is likely to be rapidly metabolised. In conclusion, this mechanism could provide a route by which nitrite, acting as a substrate, could be reduced to NO and form other, more biologically accessible species.</p>","PeriodicalId":100151,"journal":{"name":"Autonomic and Autacoid Pharmacology","volume":"29 3","pages":"105"},"PeriodicalIF":0.0,"publicationDate":"2009-06-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/j.1474-8673.2009.00439.x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73784971","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}