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The effect of boric acid on acethylcholine, bethanechol and potasssium-evoked responses on ileum of rat 硼酸对大鼠回肠乙酰胆碱、乙酰胆碱和钾诱发反应的影响
Pub Date : 2011-09-26 DOI: 10.1111/j.1474-8673.2011.00466.x
S. Ince, R. Turkmen, H. Yavuz

1 The aim of this study was to clarify the effect of boric acid on contractions of rat isolated ileum.

2 Contractile responses expressed as Emax and pD2 for acetylcholine (10−3–10−8m, Ach), bethanechol (10−3–10−8m) and potassium (10–80 × 10−3m, KCl) were determined in the absence and presence of boric acid (10−3; 5 × 10−4; 10−4m).

3 The contractile response to Ach in the presence of verapamil (10−6 or 10−8m) or in calcium-free Tyrode’s solution was also determined in the absence and presence of boric acid.

4 Boric acid did not affect the contractile response to Ach, bethanechol or KCl. Single or cumulative treatment of boric acid did not affect ileum muscle contraction evoked by KCl. The atropine-resistant component of Ach-induced contraction and 4-diphenyl-acetoxy-N-methyl-piperidine methiodide-resistant component of bethanechol-induced contraction were not inhibited by boric acid (10−3m). The contractile response to Ach was reduced in calcium-free Tyrode’s solution, and the contractile response was not affected by (10−8m). The addition of boric acid (10−3m) in combination with verapamil (10−8m) did not significantly affect the contractile response to Ach.

5 In conclusion, boric acid does not affect contractions induced by Ach, bethanechol or potassium in rat isolated ileum.

本研究的目的是阐明硼酸对大鼠离体回肠收缩的影响。2在不存在和存在硼酸(10−3;5 × 10−4;10−4米)。在维拉帕米(10−6或10−8 m)或无钙Tyrode’s溶液中,也测定了在没有硼酸和硼酸存在的情况下,乙酰胆碱的收缩反应。硼酸对乙酰胆碱、乙二酚和氯化钾的收缩反应没有影响。硼酸单次或累积处理对氯化钾引起的回肠肌收缩无影响。硼酸(10 ~ 3 m)对乙酰胆碱诱导的收缩的抗阿托品成分和4-二苯基-乙酰氧基- n -甲基哌啶抗甲氧胺成分没有抑制作用。在无钙Tyrode’s溶液中,对Ach的收缩反应降低,且(10−8 m)对收缩反应没有影响。硼酸(10 ~ 3 m)与异搏米(10 ~ 8 m)联合添加对乙酰胆碱的收缩反应无显著影响。5由此可见,硼酸不影响乙酰胆碱、亚丁酚或钾诱导的大鼠离体回肠收缩。
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引用次数: 6
Angiotensin II modifies the expression of α1-adrenoceptors in aorta smooth muscle cells of α1D-adrenoceptor knockout mice 血管紧张素II改变α 1d -肾上腺素受体敲除小鼠主动脉平滑肌细胞中α1-肾上腺素受体的表达
Pub Date : 2011-09-26 DOI: 10.1111/j.1474-8673.2011.00467.x
M. L. Lázaro-Suárez, J. H. Gómez-Zamudio, N. L. Delgado-Buenrostro, A. Tanoue, G. Tsujimoto, R. Villalobos-Molina

1 The effect of angiotensin II (Ang II) on α1A-, α1B-, and a1D-adrenoceptors (α1-AR) expression was analyzed in aorta smooth muscle cells obtained from wild-type (WT) and knock out of α1D-AR (α1D-AR KO) mice.

2 The relative abundance of mRNA for the three α1-ARs was determined in WT and α1D-AR KO aortic smooth muscle cells. There were no significant differences between WT and α1D-AR KO cells.

3 As early as 1 h Ang II increased α1B-AR mRNA in WT cells ≈ 2 fold compared with control; in contrast, in α1D-AR KO cells the α1B-AR transcript was ≈ 50% of control.

4 Western blot assays showed that Ang II incremented protein content for α1A-AR, 86% and 107% in WT and α1D-AR KO cells, respectively.

5 Protein for α1B- and α1D-ARs did not change significantly with Ang II in both WT and a1D-AR KO cells.

6 The effect of Ang II on α1B-AR mRNA seems to be influenced by the absence of α1D-AR in aortic smooth muscle cells, which might be important to understand the interactions among α1-ARs.

1 .分析血管紧张素II (Ang II)对α1D-AR (α1D-AR KO)野生型和敲除型小鼠主动脉平滑肌细胞α1A-、α1B-和α 1d -肾上腺素受体(α1-AR)表达的影响。2测定WT和α1D-AR KO主动脉平滑肌细胞中3种α1- ar mRNA的相对丰度。WT与α1D-AR KO细胞间差异无统计学意义。3早在1 h时,Angⅱ使WT细胞α1B-AR mRNA表达量较对照增加约2倍;而在α1D-AR KO细胞中,α1B-AR转录物约为对照组的50%。4 . Western blot检测显示,angii使WT和α1D-AR - KO细胞中α1A-AR蛋白含量分别增加了86%和107%。5在WT和a1D-AR KO细胞中α1B-和α1D-ARs蛋白均未随Ang II发生显著变化。6 angii对α1B-AR mRNA的影响可能受到主动脉平滑肌细胞中α1D-AR缺失的影响,这可能对了解α1- ar之间的相互作用具有重要意义。
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引用次数: 2
Pharmacodynamic interaction between pantoprazole and vecuronium at neuromuscular junction 泮托拉唑与维库溴铵在神经肌肉连接处的药效学相互作用
Pub Date : 2011-02-23 DOI: 10.1111/j.1474-8673.2011.00463.x
V. K. Vadgama, Y. A. Patel, T. K. Patel, C. B. Tripathi

1 The effect of pantoprazole on vecuronium-induced neuromuscular blockade in in vivo has not been clearly defined. In this study, we demonstrate that chronic administration, but not acute administration, of pantoprazole alters the pattern of vecuronium-induced neuromuscular blockade.

2 This study was designed to evaluate the effect of acute and chronic administration of pantoprazole on vecuronium-induced neuromuscular blockade using the rat in vivo sciatic nerve-stimulated gastrocnemius preparation.

3 Vecuronium was administered as a slow intravenous infusion (29.41 μg kg−1 min−1) until the gastrocnemius twitch response to sciatic nerve stimulation was completely abolished. The effect of acute (single dose, i.v.) and chronic administration (per oral for 21 days) of pantoprazole (3.64 mg kg−1) on vecuronium-induced blockade was assessed by comparing ED50 values, time required for 50% block, ED95 values, block duration and percentage of recovery with respect to control.

4 Acute administration of pantoprazole had no significant effect on any parameter of vecuronium-induced neuromuscular blockade. Chronic administration of pantoprazole significantly reduced vecuronium ED50 value, time for 50% block, ED95 value and percentage recovery from blockade compared with the control group (P < 0.05). Reduction in the duration of vecuronium-induced blockade was not significantly affected by chronic treatment with pantoprazole compared with control.

5 On chronic administration, pantoprazole may produce earlier block, quick relaxation and reduces the recovery of vecuronium without affecting its duration of action.

1 .泮托拉唑在体内对维库溴铵诱导的神经肌肉阻断的作用尚未明确。在这项研究中,我们证明慢性给药,而不是急性给药,泮托拉唑改变维库溴铵诱导的神经肌肉阻滞模式。2本研究旨在通过大鼠体内坐骨神经刺激腓肠肌制剂,评估急性和慢性给药泮托拉唑对维库溴铵诱导的神经肌肉阻滞的影响。静脉缓慢滴注维库溴铵(29.41 μg kg−1 min−1),直至腓肠肌抽搐对坐骨神经刺激的反应完全消除。通过比较ED50值、50%阻断所需时间、ED95值、阻断持续时间和相对于对照组的恢复百分比,评估泮托拉唑(3.64 mg kg - 1)急性(单剂量,静脉注射)和慢性(每次口服21天)对vecuronium诱导阻断的影响。4 .急性给药泮托拉唑对维库溴铵诱导的神经肌肉阻滞各参数均无显著影响。与对照组相比,长期给药泮托拉唑显著降低维库溴铵ED50值、阻断50%时间、ED95值和阻断后恢复百分比(P < 0.05)。与对照组相比,泮托拉唑慢性治疗对维库onium诱导的阻断持续时间的减少没有显著影响。长期给药时,泮托拉唑可产生早期阻滞、快速松弛和减少维库溴铵的恢复,而不影响其作用时间。
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引用次数: 1
Colon-specific contractile responses to tetrodotoxin in the isolated mouse gastrointestinal tract 离体小鼠胃肠道对河豚毒素的结肠特异性收缩反应
Pub Date : 2011-02-18 DOI: 10.1111/j.1474-8673.2011.00462.x
Y. Okuno, T. Kondo, A. Saeki, E. Uchida, H. Teraoka, T. Kitazawa

1 Tetrodotoxin (TTX) is a useful pharmacological tool for distinguishing neural and myogenic responses of isolated visceral organs to drugs. Although TTX does not generally affect smooth muscle tonus, in this study, we have found that TTX causes contraction of the mouse colon. The aim of this study was to characterize this TTX-induced contraction in the mouse gastrointestinal tract.

2 Longitudinal and circular muscle strips from the stomach and small intestine were less sensitive to TTX. However, TTX contracted both smooth muscle strips from the proximal colon and distal colon.

3 Pretreatment with TTX, Nω-nitro-l-arginine methyl ester (l-NAME), (1)H-[1,2,4]oxadiazolo[4,3-a]quinoxalin-1-one (ODQ) and apamin inhibited the TTX-induced contraction. l-NAME, ODQ or apamin itself caused contraction in the colon but not in the gastric and small intestinal strips. Region dependency of l-NAME, ODQ and apamin-induced contraction correlated with that of TTX-induced contraction.

4 l-Arginine but not d-arginine inhibited contractility of the colonic strips without affecting the contractility of muscle strips from other regions. Sodium nitroprusside caused strong relaxation of the colonic strips.

5 1,1-Dimethyl-4-phenylpiperazinium (DMPP) caused relaxation of proximal and distal colons, which was significantly decreased by l-NAME or apamin.

6 In conclusion, among mouse gastrointestinal preparations, TTX induces contraction of colonic strips preferentially through blockade of potent tonic inhibitory neural outflow, which involves nitrergic and apamin-sensitive pathways. Colon-specific responses to l-arginine, l-NAME, ODQ and apamin support the hypothesis that there is a continuous suppression of colonic motility by enteric inhibitory neurons.

河豚毒素(TTX)是区分离体内脏器官对药物的神经和肌源性反应的有用药理学工具。虽然TTX一般不影响平滑肌张力,但在本研究中,我们发现TTX引起小鼠结肠收缩。本研究的目的是表征这种ttx诱导的小鼠胃肠道收缩。2 .胃和小肠纵向和圆形肌条对TTX的敏感性较低。然而,TTX收缩了近端结肠和远端结肠的平滑肌条。3 TTX、n ω-硝基-l-精氨酸甲酯(l-NAME)、(1)H-[1,2,4]恶二唑[4,3-a]喹诺沙林-1-酮(ODQ)和apamin预处理可抑制TTX诱导的收缩。l-NAME、ODQ或维生素a本身引起结肠收缩,而不是胃和小肠条收缩。l-NAME、ODQ和apamine诱导的收缩的区域依赖性与ttx诱导的收缩相关。l-精氨酸抑制结肠肌条的收缩力,而d-精氨酸不影响其他部位肌条的收缩力。硝普钠引起结肠条带的强烈松弛。1,1-二甲基-4-苯基哌嗪(DMPP)引起近端和远端结肠松弛,l-NAME或apamin显著降低。综上所述,在小鼠胃肠道制剂中,TTX通过阻断强效强直抑制性神经流出来优先诱导结肠条收缩,这涉及到氮能和氨基敏感通路。结肠对l-精氨酸、l-NAME、ODQ和维生素d的特异性反应支持了肠抑制神经元持续抑制结肠运动的假设。
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引用次数: 10
The effect of losartan and carvedilol on vasopressor responses to adrenergic agonists and angiotensin II in the systemic circulation of Sprague Dawley rats 氯沙坦和卡维地洛对Sprague Dawley大鼠体循环肾上腺素能激动剂和血管紧张素II血管加压反应的影响
Pub Date : 2010-12-20 DOI: 10.1111/j.1474-8673.2010.00461.x
M. H. Abdulla, M. A. Sattar, N. A. Abdullah, M. A. H. Khan, K. R. L. Anand Swarup, E. J. Johns

1 Interaction between renin-angiotensin (RAS) and sympathetic nervous systems (SNS) was investigated by examining the effect of cumulative blockade of angiotensin II (Ang II) and adrenergic receptors in normal Sprague Dawley rats.

2 Rats were treated with losartan (10 mg/kg), carvedilol (5 mg/kg), or losartan plus carvedilol (10 + 5 mg/kg) orally for 7 days. On day 8, the animals were anaesthetized with pentobarbitone and prepared for systemic haemodynamic study. Dose–response relationships for the elevation of mean arterial pressure or change in heart rate (HR) in response to intravenous injections of noradrenaline (NA), phenylephrine (PE), methoxamine (ME) and Ang II were determined.

3 Losartan or the combination of losartan with carvedilol blunted vasopressor responses to ME and Ang II. Dose–response relationships for agonist action on HR were significantly inhibited by all treatments except for the combination of losartan and carvedilol on the decrease in HR induced by PE. Carvedilol decreased vasopressor responses to NA, PE and Ang II, and HR responses to NA, ME and Ang II. Combination treatment produced similar effects to losartan on the vasopressor and HR responses but had a greater effect on vasopressor responses to ME and Ang II, and on HR responses to NA and Ang II than carvedilol alone.

4 It is concluded that peripheral vasoconstriction induced by Ang II is partly mediated by adrenergic action and that the vasopressor responses to adrenergic agonists depend on an intact RAS. These observations suggest an interactive relationship between RAS and SNS in determining systemic haemodynamic responses in ‘normal’ rats.

1通过对正常大鼠血管紧张素II (Ang II)和肾上腺素能受体的累积阻断,探讨肾素-血管紧张素(RAS)与交感神经系统(SNS)的相互作用。2大鼠口服氯沙坦(10 mg/kg)、卡维地洛(5 mg/kg)或氯沙坦+卡维地洛(10 + 5 mg/kg),连续7 d。第8天,用戊巴比酮麻醉动物,准备进行全身血流动力学研究。测定静脉注射去甲肾上腺素(NA)、苯肾上腺素(PE)、甲氧苄胺(ME)和Angⅱ后平均动脉压升高或心率(HR)变化的剂量-反应关系。氯沙坦或氯沙坦与卡维地洛联用可减弱ME和angii的血管加压反应。除氯沙坦与卡维地洛联用外,所有治疗均显著抑制了激动剂对肺活量的影响。卡维地洛降低了NA、PE和Ang II的血管加压反应,降低了NA、ME和Ang II的HR反应。联合治疗对血管加压剂和HR反应的影响与氯沙坦相似,但对ME和Ang II的血管加压剂反应以及NA和Ang II的HR反应的影响比单独卡维地洛更大。由此得出结论,Ang II诱导的外周血管收缩部分是由肾上腺素能作用介导的,而肾上腺素能激动剂的血管加压反应依赖于完整的RAS。这些观察结果表明RAS和SNS在决定“正常”大鼠的全身血流动力学反应方面存在相互作用关系。
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引用次数: 5
Characterization of β-adrenoceptor-mediated relaxation signals in isolated pulmonary artery of Dahl salt-sensitive hypertensive and normotensive rats 达尔盐敏感型高血压和正常高血压大鼠离体肺动脉β-肾上腺素受体介导的舒张信号的表征
Pub Date : 2010-10-19 DOI: 10.1111/j.1474-8673.2010.00460.x
C. A. Ford, P. Mahajan, R. Tabrizchi

1 Relaxant responses to isoprenaline (ISO) were studied in the pulmonary arteries of normotensive and hypertensive Dahl salt-sensitive rats. Rats were fed either a high-salt (4.0%) or low-salt (0.14%) diet for 5 weeks. Animals fed a high-salt diet (167/123 ± 2/2 mmHg) had a significantly higher blood pressure compared to those fed a low-salt diet (127/87 ± 2/2 mmHg).

2 Isoprenaline-elicited relaxations were not significantly different in tissues from hypertensive compared to normotensive animals. Responses to ISO were significantly attenuated in denuded tissues and substantially more so in hypertensive compared to normotensive animals. While relaxant responses to ISO were resistant to inhibition by Nω-nitro-l-arginine methyl ester, indomethacin, glibenclamide or a combination of barium chloride and ouabain, they were inhibited by Rp-cAMP, anandamide and acidic buffer. The inhibitory impact of anandamide and acidic buffer was significantly greater in tissues from hypertensive vs. normotensive rats.

3 The resting membrane potential (Em) of smooth muscle cells was −67.0 ± 0.7 mV (n = 43 cells) and −66.6 ± 0.8 mV (n = 55 cells) in pulmonary arteries from hypertensive and normotensive rats, respectively. Isoprenaline produced hyperpolarization of Em which was significant in the blood vessels of hypertensive (−71.6 ± 0.8 mV; n = 29 cells) but not normotensive (−68.1 ± 0.7 mV; n = 49 cells) rats.

4 The endothelium plays a critical role in β-adrenoceptor-mediated relaxation but nitric oxide is not the mediator for the response. It is possible that the greater hyperpolarization caused by ISO in blood vessels from hypertensive compared to normotensive rats is mediated by activation of TASK-1 channels.

研究了正常和高血压达尔盐敏感大鼠肺动脉对异丙肾上腺素(ISO)的松弛反应。大鼠分别饲喂高盐(4.0%)和低盐(0.14%)饮食5周。高盐饮食(167/123±2/2 mmHg)的动物血压明显高于低盐饮食(127/87±2/2 mmHg)的动物。与正常血压动物相比,高血压动物组织中异丙肾上腺碱引起的松弛无显著差异。与正常动物相比,剥落组织对ISO的反应明显减弱,高血压动物对ISO的反应明显减弱。对ISO的松弛反应对ω-硝基-l-精氨酸甲酯、吲哚美辛、格列本脲或氯化钡与瓦巴因联合用药有抑制作用,而Rp-cAMP、阿南胺和酸性缓冲液对其有抑制作用。在高血压大鼠组织中,阿南德胺和酸性缓冲液的抑制作用明显大于正常大鼠。肺动脉平滑肌细胞静息膜电位(Em)分别为- 67.0±0.7 mV (n = 43个细胞)和- 66.6±0.8 mV (n = 55个细胞)。异丙肾上腺素在高血压患者血管中引起Em超极化(−71.6±0.8 mV;n = 29个细胞),但不正常(−68.1±0.7 mV;N = 49个细胞)。内皮在β-肾上腺素受体介导的松弛中起关键作用,但一氧化氮不是反应的介质。与正常大鼠相比,高血压大鼠血管中ISO引起的更大的超极化可能是由TASK-1通道的激活介导的。
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引用次数: 3
Extraendothelial and constitutive COX-2 expression is involved in the contractile effect of angiotensin II in the rat aorta 内皮外和构成性COX-2表达参与大鼠主动脉血管紧张素II的收缩作用
Pub Date : 2010-07-05 DOI: 10.1111/j.1474-8673.2010.00457.x
M. C. Castillo-Hernández, M. A. Martinez-Godinez, G. Guevara-Balcazar, A. Miliar-Garcia, J. Mancilla, R. M. Lopez-Mayorga, E. F. Castillo-Henkel, C. Castillo-Henkel

1 The role of the extraendothelial and constitutive isoforms of cyclo-oxygenase-2 (COX-2) in the contractile effect of angiotensin II (Ang II) was investigated using thoracic and abdominal aortic rings without endothelium from young Wistar rats.

2 Ang II elicited similar contractions in both aortic segments, and the effect was inhibited by pretreatment with NS398 (a selective COX-2 inhibitor) but not SC-560 [selective cyclo-oxygenase-1 (COX-1) inhibitor].

3 COX-2 mRNA was expressed under basal conditions in both aortic segments. Additionally, Ang II increased COX-2 mRNA expression in the abdominal but not the thoracic segment, while cycloheximide (a protein synthesis inhibitor) did not affect the contractile response to Ang II in either of the two segments; this suggests that the effect is not associated with de novo COX-2 synthesis.

4 In conclusion, the basal amount of COX-2 found in aortic smooth muscle cells is sufficient to explain the production of the prostanoids related to the contractile effect of Ang II. The production of these prostanoids, which are derived from constitutive COX-2, occurs independently of the endothelium vascular system.

1利用年轻Wistar大鼠无内皮的胸腹主动脉环,研究了环氧化酶-2 (COX-2)内皮外和组成型异构体在血管紧张素II (Ang II)收缩作用中的作用。2 Ang II在两个主动脉段引起类似的收缩,并且用NS398(一种选择性COX-2抑制剂)预处理可以抑制这种作用,但SC-560(一种选择性环氧化酶-1 (COX-1)抑制剂)不能抑制这种作用。3 COX-2 mRNA在基底条件下均有表达。此外,Ang II增加了腹部而不是胸部的COX-2 mRNA表达,而环己亚胺(一种蛋白质合成抑制剂)不影响两个节段对Ang II的收缩反应;这表明该效应与从头合成COX-2无关。综上所述,在主动脉平滑肌细胞中发现的COX-2的基础量足以解释与Ang II收缩作用相关的前列腺素的产生。这些前列腺素的产生,来源于组成型COX-2,独立于内皮血管系统发生。
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引用次数: 2
Effects of repeated maprotiline and fluoxetine treatment on gene expression of catecholamine synthesizing enzymes in adrenal medulla of unstressed and stressed rats 马普替林和氟西汀对应激大鼠肾上腺髓质儿茶酚胺合成酶基因表达的影响
Pub Date : 2010-07-05 DOI: 10.1111/j.1474-8673.2010.00458.x
N. Spasojevic, L. Gavrilovic, S. Dronjak

1 Repeated maprotiline (a noradrenaline reuptake inhibitor) and fluoxetine (a serotonin reuptake inhibitor) treatment on gene expression of catecholamine biosynthetic enzymes were examined in adrenal medulla of unstressed control and chronic unpredictable mild stressed rats.

2 Maprotiline did not change gene expression of catecholamine biosynthetic enzymes in control and stressed rats.

3 Fluoxetine increased gene expression of tyrosine hydroxylase (TH) and dopamine-β-hydroxylase (DBH), but did not phenylethanolamine N-methyltransferase in both unstressed and chronic unpredictable mild stressed animals.

4 In conclusion, we have demonstrated that repeated administration of fluoxetine enhanced gene transcription of TH and DBH and subsequently stimulates noradrenaline synthesis in adrenal medulla of control and stressed rats.

研究了马普替林(一种去甲肾上腺素再摄取抑制剂)和氟西汀(一种血清素再摄取抑制剂)对非应激对照和慢性不可预测轻度应激大鼠肾上腺髓质儿茶酚胺生物合成酶基因表达的影响。2 .马普替林对对照组和应激大鼠儿茶酚胺生物合成酶基因表达无影响。3氟西汀增加了非应激和慢性不可预测轻度应激动物酪氨酸羟化酶(TH)和多巴胺-β-羟化酶(DBH)的基因表达,但对苯乙醇胺n -甲基转移酶没有影响。综上所述,我们已经证明,反复给药氟西汀增强了TH和DBH的基因转录,随后刺激了对照组和应激大鼠肾上腺髓质中去甲肾上腺素的合成。
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引用次数: 18
Angiotensin-II type 1 receptor (AT1R) and alpha-1D adrenoceptor form a heterodimer during pregnancy-induced hypertension 血管紧张素ii型1受体(AT1R)和α - 1d肾上腺素受体在妊娠高血压期间形成异源二聚体
Pub Date : 2010-06-09 DOI: 10.1111/j.1474-8673.2009.00446.x
M. de Lourdes González-Hernández, D. Godínez-Hernández, R. A. Bobadilla-Lugo, P. López-Sánchez

1 Pregnancy courses with low response to angiotensin II and adrenergic agonists. In preeclampsia, both effects are reverted. It is known that angiotensin II regulates adrenergic system. It is not known, however, the interaction between both systems receptors.

2 Our aim was to study if AT1R and α1D adrenoceptor heterodimerize in preeclampsia.

3 We used subrenal aorctic coarctation in pregnant rats. Aortic tissues were prepared for confocal imaging and coimmunoprecipitated for α1D and AT1 receptors.

4 We found that AT1R and α1D adrenoceptor heterodimerize in both, healthy and preeclamptic groups. In healthy pregnant rats, heterodimer is barely detected. In preeclamptic rats however, we found higher heterodimerization.

5 These results suggest that AT1R and α1D -adrenoceptor may form heterodimers, and may play a role in preeclampsia.

妊娠期对血管紧张素II和肾上腺素激动剂反应低。在子痫前期,这两种作用都被逆转了。众所周知,血管紧张素II调节肾上腺素能系统。然而,这两个系统受体之间的相互作用尚不清楚。我们的目的是研究AT1R和α1D肾上腺素受体在子痫前期是否异二聚。我们采用妊娠大鼠肾下主动脉缩窄术。制备主动脉组织进行共聚焦成像,并对α1D和AT1受体进行共免疫沉淀。我们发现AT1R和α1D肾上腺素受体在健康组和子痫前期组中均存在异二聚体。在健康的怀孕大鼠中,几乎检测不到异二聚体。然而,在子痫前期大鼠中,我们发现较高的异源二聚化。这些结果提示AT1R与α1D -肾上腺素受体可能形成异源二聚体,并可能在子痫前期发挥作用。
{"title":"Angiotensin-II type 1 receptor (AT1R) and alpha-1D adrenoceptor form a heterodimer during pregnancy-induced hypertension","authors":"M. de Lourdes González-Hernández,&nbsp;D. Godínez-Hernández,&nbsp;R. A. Bobadilla-Lugo,&nbsp;P. López-Sánchez","doi":"10.1111/j.1474-8673.2009.00446.x","DOIUrl":"10.1111/j.1474-8673.2009.00446.x","url":null,"abstract":"<div>\u0000 \u0000 <p> <b>1</b> Pregnancy courses with low response to angiotensin II and adrenergic agonists. In preeclampsia, both effects are reverted. It is known that angiotensin II regulates adrenergic system. It is not known, however, the interaction between both systems receptors.</p>\u0000 <p> <b>2</b> Our aim was to study if AT<sub>1</sub>R and α1D adrenoceptor heterodimerize in preeclampsia.</p>\u0000 <p> <b>3</b> We used subrenal aorctic coarctation in pregnant rats. Aortic tissues were prepared for confocal imaging and coimmunoprecipitated for α1D and AT<sub>1</sub> receptors.</p>\u0000 <p> <b>4</b> We found that AT<sub>1</sub>R and α1D adrenoceptor heterodimerize in both, healthy and preeclamptic groups. In healthy pregnant rats, heterodimer is barely detected. In preeclamptic rats however, we found higher heterodimerization.</p>\u0000 <p> <b>5</b> These results suggest that AT<sub>1</sub>R and α1D -adrenoceptor may form heterodimers, and may play a role in preeclampsia.</p>\u0000 </div>","PeriodicalId":100151,"journal":{"name":"Autonomic and Autacoid Pharmacology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2010-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/j.1474-8673.2009.00446.x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"28672844","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 29
Effects of long-term treatment with fluoxetine and venlafaxine on rat isolated vas deferens 氟西汀和文拉法辛长期治疗对大鼠离体输精管的影响
Pub Date : 2010-06-09 DOI: 10.1111/j.1474-8673.2010.00456.x
S. S. Göçmez, T. Utkan, G. Ulak, N. Gacar, F. Erden

1 Antidepressant therapy is considered as one of the factors leading to male infertility.

2 In this study, the effects of long-term treatment with fluoxetine or venlafaxine were investigated on electrical field stimulation (EFS, 1–64 Hz), noradrenaline (10−8 to 10−4m), serotonin (10−8 to 10−4m), adenosine 5′-triphosphate [ATP (10−8 to 10−4m)] and 80 mm KCl-induced contractile responses in the epididymal and prostatic portions of rat isolated vas deferens strips.

3 Serotonin-induced contractile responses were significantly increased in the epididymal portion of the vas deferens obtained from the fluoxetine-treatment group, whereas in the prostatic portion there was no change. However, venlafaxine treatment had no effect on serotonin responses in the either portion of the vas deferens. Both fluoxetine and venlafaxine treatment significantly inhibited ATP-evoked contractions of the prostatic and epididymal portions of the rat vas deferens, but had no effect on EFS, noradrenaline- and KCl-evoked contractions of the vas deferentia in both portions.

4 In conclusion, these results suggest that chronic treatment with fluoxetine and venlafaxine affects vas deferens motility. Purinoceptors may, at least in part, responsible for the impaired motility in chronic treatment of venlafaxine and fluoxetine.

抗抑郁治疗被认为是导致男性不育的因素之一。在这项研究中,研究了氟西汀或文拉法辛长期治疗对电场刺激(EFS, 1-64 Hz)、去甲肾上腺素(10−8至10−4 m)、血清素(10−8至10−4 m)、腺苷5′-三磷酸[ATP(10−8至10−4 m)]和80 mm氯化钾诱导的大鼠离体输精管条附睾和前列腺部分的收缩反应的影响。氟西汀治疗组输精管附睾部分血清素诱导的收缩反应明显增加,而前列腺部分没有变化。然而,文拉法辛治疗对输精管两部分的血清素反应没有影响。氟西汀和文拉法辛治疗均能显著抑制atp诱导的大鼠输精管前列腺和附睾部分的收缩,但对EFS、去甲肾上腺素和kcl诱导的输精管两部分的收缩无影响。综上所述,这些结果表明氟西汀和文拉法辛慢性治疗影响输精管运动。嘌呤受体可能,至少部分地,对文拉法辛和氟西汀慢性治疗中的运动障碍负责。
{"title":"Effects of long-term treatment with fluoxetine and venlafaxine on rat isolated vas deferens","authors":"S. S. Göçmez,&nbsp;T. Utkan,&nbsp;G. Ulak,&nbsp;N. Gacar,&nbsp;F. Erden","doi":"10.1111/j.1474-8673.2010.00456.x","DOIUrl":"10.1111/j.1474-8673.2010.00456.x","url":null,"abstract":"<div>\u0000 \u0000 <p> <b>1</b> Antidepressant therapy is considered as one of the factors leading to male infertility.</p>\u0000 <p> <b>2</b> In this study, the effects of long-term treatment with fluoxetine or venlafaxine were investigated on electrical field stimulation (EFS, 1–64 Hz), noradrenaline (10<sup>−8</sup> to 10<sup>−4</sup> <span>m</span>), serotonin (10<sup>−8</sup> to 10<sup>−4</sup> <span>m</span>), adenosine 5′-triphosphate [ATP (10<sup>−8</sup> to 10<sup>−4</sup> <span>m</span>)] and 80 m<span>m</span> KCl-induced contractile responses in the epididymal and prostatic portions of rat isolated vas deferens strips.</p>\u0000 <p> <b>3</b> Serotonin-induced contractile responses were significantly increased in the epididymal portion of the vas deferens obtained from the fluoxetine-treatment group, whereas in the prostatic portion there was no change. However, venlafaxine treatment had no effect on serotonin responses in the either portion of the vas deferens. Both fluoxetine and venlafaxine treatment significantly inhibited ATP-evoked contractions of the prostatic and epididymal portions of the rat vas deferens, but had no effect on EFS, noradrenaline- and KCl-evoked contractions of the vas deferentia in both portions.</p>\u0000 <p> <b>4</b> In conclusion, these results suggest that chronic treatment with fluoxetine and venlafaxine affects vas deferens motility. Purinoceptors may, at least in part, responsible for the impaired motility in chronic treatment of venlafaxine and fluoxetine.</p>\u0000 </div>","PeriodicalId":100151,"journal":{"name":"Autonomic and Autacoid Pharmacology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2010-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/j.1474-8673.2010.00456.x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"29069623","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 16
期刊
Autonomic and Autacoid Pharmacology
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