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Biochimica et Biophysica Acta (BBA) - Specialized Section on Lipids and Related Subjects最新文献

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The action of vitamin D in vivo and in vitro on the incorporation of Dl-[3-14C]serine into phosphatidylserine 体内外维生素D对Dl-[3-14C]丝氨酸并入磷脂酰丝氨酸的作用
Biochimica et Biophysica Acta (BBA) - Specialized Section on Lipids and Related Subjects
Pub Date : 1964-12-02 DOI: 10.1016/0926-6542(64)90041-1
Norimasa Hosoya, Tomino Watanabe, Asao Fujimori
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引用次数: 10
Stimulation of lipid biosynthesis in sympathetic and sensory ganglia by a specific nerve growth factor 特定神经生长因子刺激交感神经节和感觉神经节脂质生物合成
Biochimica et Biophysica Acta (BBA) - Specialized Section on Lipids and Related Subjects
Pub Date : 1964-12-02 DOI: 10.1016/0926-6542(64)90045-9
P.U. Angeletti , A. Liuzzi , R. Levi-Montalcini
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引用次数: 18
Free fatty acids as lipogenic inhibitors 游离脂肪酸作为脂肪生成抑制剂
Biochimica et Biophysica Acta (BBA) - Specialized Section on Lipids and Related Subjects
Pub Date : 1964-12-02 DOI: 10.1016/0926-6542(64)90034-4
H.M. Korchak , E.J. Masoro
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引用次数: 44
Metabolism in vitro of palmitic acid and linoleic acid in the heart and diaphragm of essential fatty acid-deficient rats 棕榈酸和亚油酸在必需脂肪酸缺乏大鼠心脏和膈肌的体外代谢
Biochimica et Biophysica Acta (BBA) - Specialized Section on Lipids and Related Subjects
Pub Date : 1964-12-02 DOI: 10.1016/0926-6542(64)90022-8
Olga Stein , Yecheskiel Stein

Rats kept for 75–90 days on an essential fatty acid-deficient diet, showed a marked fall in linoleic acid content and a rise in the trienoic acid/tetreanoic acid ratio in heart as well as in diaphragm. Electron microscopic study of myocardium showed enlarged mitochondria with marked disorganization of cristae and swelling. Neutral lipid and phospholipid content of heart and diaphragm was not different from that of controls, supplemented with 4% soya-bean oil. Rat hearts were perfused and hemidiaphragms were incubated with a medium containing [1-14C]palmitic or [1-14C]linoleic acid. During 20 min of perfusion both fatty acids were oxidized to CO2 to a similar extent. The fatty acid composition of the medium affected the mode and rate of incorporation of individual fatty acids in heart as well as diaphragm. In the presence of an equipmolar mixture of palmitic and linoleic acid, more linoleic acid was incorporated into phospholipids and more palmitic into neutral lipids, than when each fatty acid was the only substrate. In the essential fatty acid-deficient rats the specific activity of the phospholipids synthesized with [1-14C linoleic acid in heart as well as in diaphragm was higher than in controls. An increased turnover of certain fatty acids in phospholipids in the essential fatty acid-deficient rats is indicated and the mechanism discussed.

饲喂缺乏必需脂肪酸饮食75-90天的大鼠，心脏和膈肌的亚油酸含量明显下降，三烯酸/四烯酸比值明显上升。电镜观察心肌线粒体增大，嵴明显紊乱，肿胀。添加4%大豆油后，心脏和膈肌中性脂和磷脂含量与对照组无显著差异。大鼠心脏灌注，半隔膜用含有[1-14C]棕榈酸或[1-14C]亚油酸的培养基孵育。在灌注20 min时，两种脂肪酸被氧化为CO2的程度相似。培养基的脂肪酸组成影响个别脂肪酸在心脏和隔膜的掺入方式和速率。在棕榈酸和亚油酸的混合物中，与每种脂肪酸作为唯一底物相比，更多的亚油酸被掺入磷脂中，更多的棕榈酸被掺入中性脂中。必需脂肪酸缺乏大鼠心脏和膈肌中[1-14C亚油酸合成的磷脂比活性均高于对照组。必需脂肪酸缺乏大鼠的磷脂中某些脂肪酸的周转增加，并讨论了其机制。
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引用次数: 26
Effect of dietary fats on the fatty acid composition of liver and bile phosphatides of hamsters 饲粮脂肪对仓鼠肝脏和胆汁磷脂脂肪酸组成的影响
Biochimica et Biophysica Acta (BBA) - Specialized Section on Lipids and Related Subjects
Pub Date : 1964-12-02 DOI: 10.1016/0926-6542(64)90035-6
Joseph L. Glenn , Frede Christens, Henrik Dam
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引用次数: 17
Prevention of cholesterol esterification in human serum by enzymic degradation of phospholipids 磷脂酶降解预防人血清胆固醇酯化
Biochimica et Biophysica Acta (BBA) - Specialized Section on Lipids and Related Subjects
Pub Date : 1964-12-02 DOI: 10.1016/0926-6542(64)90038-1
Robert Rowen
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引用次数: 9
Tissue levels of acid-insoluble carnitine in rat heart 大鼠心脏中酸不溶性肉碱的组织水平
Biochimica et Biophysica Acta (BBA) - Specialized Section on Lipids and Related Subjects
Pub Date : 1964-12-02 DOI: 10.1016/0926-6542(64)90042-3
D.J. Pearson, P.K. Tubbs
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引用次数: 39
Water extrusion in isolated subcellular fractions 水挤压分离亚细胞组分
Biochimica et Biophysica Acta (BBA) - Specialized Section on Lipids and Related Subjects
Pub Date : 1964-12-02 DOI: 10.1016/0926-6542(64)90027-7
Renzo Rendi

Suspensions of phospholipids, prepared from rat-liver mitochondria or from soya bean, extrude water when serum albumin and a Mg2+-ethylenediaminetetraacetate complex is addes. None of many other chelators tested can replace ethylenediaminetetraacetate. The pH optimum is 7.1 for the soya-bean phosphatides and 8.5 for those prepared from mitochondira. Various characteristics of the reaction are similar to the one observed in dialysed rat-liver mitochondira, although in this latter system serum albumin is not required. While in the dialyzed-mitochondira system Ca2+ can be replaced by Mg2+, with the isolated phospholipid Mg2+ is unable to replace Ca2. When dialyzed mitochondria are treate with proteolytic enzymes, the requirement for serum albumin is observed and one also finds that Mg2+ is now unable to replace Ca2+. Little decrease of water-extrusion reaction ibn dialyzed mitochondria occurs after neutral-lipid extraction, while a large decrease is seen after phospholipid removal.

当加入血清白蛋白和Mg2+-乙二胺四乙酸络合物时，从大鼠肝线粒体或大豆制备的磷脂悬浮液会挤出水。测试的许多其他螯合剂都不能取代乙二胺四乙酸酯。大豆磷脂的最适pH为7.1，线粒体磷脂的最适pH为8.5。该反应的各种特征与在透析的大鼠肝线粒体中观察到的相似，尽管在后一种系统中不需要血清白蛋白。而在透析线粒体系统中，Ca2+可以被Mg2+取代，而分离的磷脂Mg2+不能取代Ca2。当透析的线粒体用蛋白水解酶处理时，观察到对血清白蛋白的需求，并且还发现Mg2+现在不能取代Ca2+。中性脂提取后，透析线粒体的水挤压反应减少较少，而磷脂去除后，水挤压反应减少较多。
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引用次数: 14
International conference on the biochemistry of lipids 1965 1965年脂类生物化学国际会议
Biochimica et Biophysica Acta (BBA) - Specialized Section on Lipids and Related Subjects
Pub Date : 1964-12-02 DOI: 10.1016/0926-6542(64)90051-4
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引用次数: 0
The reaction of diazomethane with synthetic phosphatides and with insect lipid extracts 重氮甲烷与合成磷脂及昆虫脂质提取物的反应
Biochimica et Biophysica Acta (BBA) - Specialized Section on Lipids and Related Subjects
Pub Date : 1964-12-02 DOI: 10.1016/0926-6542(64)90025-3
H.D. Crone

  • 1.

    1. The reaction of diazomethane with synthetic phosphatidylethanolamine, N-methylphosphatidylethanolamine, phosphatidylcholine and insect-lipid extracts was studied in order to find out if the reaction was specific to one type of phosphatide.

  • 2.

    2. The three synthetic phosphatides reacted in essentially the same manner, although with different rates of reaction, forming dimethyl esters of phosphatidic acids and a nitrogenous compound similar to neurine. The results with insect extracts gave evidence of the reaction of diazomethane with many natural phosphatides.

  • 3.

    3. N-methylation of the intact ethanolamine phosphatides occurred to a limited extent and was increased by the presnece of alcohols in the reaction medium.

  • 4.

    4. Techniques used included column chromatography on silicic acid, paper chromatography and infrared spectroscopy.

1.1. 2.2研究了重氮甲烷与合成磷脂酰乙醇胺、n -甲基磷脂酰乙醇胺、磷脂酰胆碱和昆虫脂提取物的反应，以确定该反应是否对某一类磷脂具有特异性。这三种合成磷脂基本上以相同的方式反应，尽管反应速率不同，形成磷脂酸的二甲基酯和一种类似于神经氨酸的含氮化合物。用昆虫提取物的实验结果证明了重氮甲烷与许多天然磷脂的反应。完整的乙醇胺磷脂的n -甲基化在有限的程度上发生，并且由于反应介质中存在醇而增加。所使用的技术包括硅酸柱层析、纸层析和红外光谱。
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引用次数: 14
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