Pub Date : 1964-12-16DOI: 10.1016/0926-6550(64)90016-7
Sakaru Suzuki, Noriko Takahashi, Minekazu Sugiyama, Yasuo Nakanishi
{"title":"Isolation of cytidine dinucleotides from hen oviduct","authors":"Sakaru Suzuki, Noriko Takahashi, Minekazu Sugiyama, Yasuo Nakanishi","doi":"10.1016/0926-6550(64)90016-7","DOIUrl":"10.1016/0926-6550(64)90016-7","url":null,"abstract":"","PeriodicalId":100173,"journal":{"name":"Biochimica et Biophysica Acta (BBA) - Specialized Section on Nucleic Acids and Related Subjects","volume":"91 4","pages":"Pages 648-650"},"PeriodicalIF":0.0,"publicationDate":"1964-12-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0926-6550(64)90016-7","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"23813576","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1964-12-16DOI: 10.1016/0926-6550(64)90025-8
M.F. Clark
{"title":"Polyribosomes from chloroplasts","authors":"M.F. Clark","doi":"10.1016/0926-6550(64)90025-8","DOIUrl":"10.1016/0926-6550(64)90025-8","url":null,"abstract":"","PeriodicalId":100173,"journal":{"name":"Biochimica et Biophysica Acta (BBA) - Specialized Section on Nucleic Acids and Related Subjects","volume":"91 4","pages":"Pages 671-674"},"PeriodicalIF":0.0,"publicationDate":"1964-12-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0926-6550(64)90025-8","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"23813585","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1964-12-16DOI: 10.1016/0926-6550(64)90002-7
W. Karau, H.G. Zachau
Soluble RNA was isolated from brewer's yeast; on the average 30–35 g were obtained from 50 kg. 350 mg of a serine transfer-RNA fraction were isolated from 32 g of soluble RNA by several subsequent countercurrent distributions in the tri-n-butylamin system (Fig. 1, Table I). The serine transfer-RNA fraction (31 mμmoles serine/mg RNA; less than 1 % of acceptor activity for other amino acids was detected) contained two serine transfer-RNA chains. The nucleotide composition of the serine transfer-RNA fraction was determined (Fig. 3, Table II). In a second series of countercurrent distributions the two serine transfer-RNA's were separated (Fig. 2) from each other and partially purified.
{"title":"Isolierung von serinspezifischen transfer-ribonucleinsäure-fraktionen","authors":"W. Karau, H.G. Zachau","doi":"10.1016/0926-6550(64)90002-7","DOIUrl":"10.1016/0926-6550(64)90002-7","url":null,"abstract":"<div><p>Soluble RNA was isolated from brewer's yeast; on the average 30–35 g were obtained from 50 kg. 350 mg of a serine transfer-RNA fraction were isolated from 32 g of soluble RNA by several subsequent countercurrent distributions in the tri-<em>n</em>-butylamin system (Fig. 1, Table I). The serine transfer-RNA fraction (31 mμmoles serine/mg RNA; less than 1 % of acceptor activity for other amino acids was detected) contained two serine transfer-RNA chains. The nucleotide composition of the serine transfer-RNA fraction was determined (Fig. 3, Table II). In a second series of countercurrent distributions the two serine transfer-RNA's were separated (Fig. 2) from each other and partially purified.</p></div>","PeriodicalId":100173,"journal":{"name":"Biochimica et Biophysica Acta (BBA) - Specialized Section on Nucleic Acids and Related Subjects","volume":"91 4","pages":"Pages 549-558"},"PeriodicalIF":0.0,"publicationDate":"1964-12-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0926-6550(64)90002-7","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90463920","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1964-12-16DOI: 10.1016/0926-6550(64)90015-5
W. Sandritter , K. Bosselmann , L. Rakow , K. Jobst
{"title":"Untersuchungen zur feulgenreaktion die langzeithydrolyse bei verschiedenen zelltypen","authors":"W. Sandritter , K. Bosselmann , L. Rakow , K. Jobst","doi":"10.1016/0926-6550(64)90015-5","DOIUrl":"10.1016/0926-6550(64)90015-5","url":null,"abstract":"","PeriodicalId":100173,"journal":{"name":"Biochimica et Biophysica Acta (BBA) - Specialized Section on Nucleic Acids and Related Subjects","volume":"91 4","pages":"Pages 645-647"},"PeriodicalIF":0.0,"publicationDate":"1964-12-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0926-6550(64)90015-5","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83563695","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1964-12-16DOI: 10.1016/0926-6550(64)90029-5
P. Mandel , I. Borkowska
{"title":"Présence d'hybrides naturels dans les cellules d'un hépatome ascitique","authors":"P. Mandel , I. Borkowska","doi":"10.1016/0926-6550(64)90029-5","DOIUrl":"10.1016/0926-6550(64)90029-5","url":null,"abstract":"","PeriodicalId":100173,"journal":{"name":"Biochimica et Biophysica Acta (BBA) - Specialized Section on Nucleic Acids and Related Subjects","volume":"91 4","pages":"Pages 683-686"},"PeriodicalIF":0.0,"publicationDate":"1964-12-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0926-6550(64)90029-5","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75819936","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1964-12-16DOI: 10.1016/0926-6550(64)90032-5
Hsiang Ju Lin, Erwin Chargaff
{"title":"Metaphase chromosomes as a source of DNA","authors":"Hsiang Ju Lin, Erwin Chargaff","doi":"10.1016/0926-6550(64)90032-5","DOIUrl":"10.1016/0926-6550(64)90032-5","url":null,"abstract":"","PeriodicalId":100173,"journal":{"name":"Biochimica et Biophysica Acta (BBA) - Specialized Section on Nucleic Acids and Related Subjects","volume":"91 4","pages":"Pages 691-694"},"PeriodicalIF":0.0,"publicationDate":"1964-12-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0926-6550(64)90032-5","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"23813591","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1964-12-16DOI: 10.1016/0926-6550(64)90006-4
Masami Muramatsu, James L. Hodnett, Harris Busch
Using [14C]orotic acid as a precursor, the time course of labeling of nuclear and nucleolar RNA of rat liver has been studied in vivo. RNA from both nuclear and nucleolar preparations was fractionated into two classes with the aid of phenol. Each class of RNA was found to have a relatively specific kinetic profile after administration of the tracer. Labeling of nucleolar RNA was as rapid as nuclear RNA indicating that the extranucleolar RNA could not be the precursor of the nucleolar RNA. Actinomycin D inhibited nucleolar-RNA synthesis to a much greater degree than the synthesis of extranucleolar RNA. These data, together with previous observations on the base compositions of nucleolar-RNA fractions and other types of experiments provide evidence that nucleolar-RNA synthesis largely proceeds “independently” of extranucleolar-RNA synthesis.
{"title":"Studies on the “independence” of nucleolar ribonucleic acid synthesis","authors":"Masami Muramatsu, James L. Hodnett, Harris Busch","doi":"10.1016/0926-6550(64)90006-4","DOIUrl":"10.1016/0926-6550(64)90006-4","url":null,"abstract":"<div><p>Using [<sup>14</sup>C]orotic acid as a precursor, the time course of labeling of nuclear and nucleolar RNA of rat liver has been studied <em>in vivo</em>. RNA from both nuclear and nucleolar preparations was fractionated into two classes with the aid of phenol. Each class of RNA was found to have a relatively specific kinetic profile after administration of the tracer. Labeling of nucleolar RNA was as rapid as nuclear RNA indicating that the extranucleolar RNA could not be the precursor of the nucleolar RNA. Actinomycin D inhibited nucleolar-RNA synthesis to a much greater degree than the synthesis of extranucleolar RNA. These data, together with previous observations on the base compositions of nucleolar-RNA fractions and other types of experiments provide evidence that nucleolar-RNA synthesis largely proceeds “independently” of extranucleolar-RNA synthesis.</p></div>","PeriodicalId":100173,"journal":{"name":"Biochimica et Biophysica Acta (BBA) - Specialized Section on Nucleic Acids and Related Subjects","volume":"91 4","pages":"Pages 592-597"},"PeriodicalIF":0.0,"publicationDate":"1964-12-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0926-6550(64)90006-4","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"23813908","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1964-12-16DOI: 10.1016/0926-6550(64)90008-8
L. Stavy, M. Feldman, D. Elson
RNAase (polyribonucleotide 2-oligonucleotidotransferase (cyclizing), EC 2.7.7.16) activity of reticulocyte ribosomes of rabbits was tested in comparison to the nucleolytic activity of liver ribosomes. Reticulocyte ribosomes showed practically no RNAase activity under conditions where such activity was demonstrated in liver ribosomes.
{"title":"On ribonuclease activity in reticulocyte ribosomes","authors":"L. Stavy, M. Feldman, D. Elson","doi":"10.1016/0926-6550(64)90008-8","DOIUrl":"10.1016/0926-6550(64)90008-8","url":null,"abstract":"<div><p>RNAase (polyribonucleotide 2-oligonucleotidotransferase (cyclizing), EC 2.7.7.16) activity of reticulocyte ribosomes of rabbits was tested in comparison to the nucleolytic activity of liver ribosomes. Reticulocyte ribosomes showed practically no RNAase activity under conditions where such activity was demonstrated in liver ribosomes.</p></div>","PeriodicalId":100173,"journal":{"name":"Biochimica et Biophysica Acta (BBA) - Specialized Section on Nucleic Acids and Related Subjects","volume":"91 4","pages":"Pages 606-611"},"PeriodicalIF":0.0,"publicationDate":"1964-12-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0926-6550(64)90008-8","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"23813910","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1964-12-16DOI: 10.1016/0926-6550(64)90017-9
S.T. Jacob, P.M. Bhargava
{"title":"Comparison of the uracil catabolic activity of liver cell suspensions and slices","authors":"S.T. Jacob, P.M. Bhargava","doi":"10.1016/0926-6550(64)90017-9","DOIUrl":"10.1016/0926-6550(64)90017-9","url":null,"abstract":"","PeriodicalId":100173,"journal":{"name":"Biochimica et Biophysica Acta (BBA) - Specialized Section on Nucleic Acids and Related Subjects","volume":"91 4","pages":"Pages 650-652"},"PeriodicalIF":0.0,"publicationDate":"1964-12-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0926-6550(64)90017-9","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"23813577","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1964-12-16DOI: 10.1016/0926-6550(64)90019-2
Julius Gordon
{"title":"Agglutination of ribonuclease-coated red blood cells by deoxyribonucleic acid","authors":"Julius Gordon","doi":"10.1016/0926-6550(64)90019-2","DOIUrl":"10.1016/0926-6550(64)90019-2","url":null,"abstract":"","PeriodicalId":100173,"journal":{"name":"Biochimica et Biophysica Acta (BBA) - Specialized Section on Nucleic Acids and Related Subjects","volume":"91 4","pages":"Pages 655-657"},"PeriodicalIF":0.0,"publicationDate":"1964-12-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0926-6550(64)90019-2","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"23813579","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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