Pub Date : 1964-12-16DOI: 10.1016/0926-6550(64)90003-9
F. Melchers, H.G. Zachau
Several RNA samples were split with pancreatic RNAase (polyribonucleotide 2-oligonucleotidotransferase (cyclizing), EC 2.7.7.16). The fragments were separated by chromatography on DEAE-cellulose columns and in some cases by subsequent paper electrophoresis. The amounts of mono- and dinucleotides from various unfractionated and partially fractionated soluble RNA's were compared (Table I). In hydrolysates of highly purified serine transfer RNA fractions 7 di-, 7 tri-, 7 tetra-, 2 penta- and 2 hexanucleotides - coming from 2 serine transfer-RNAs - were identified and quantitatively determined (Tables II and III), as well as some oligonucleotides, which occur in smaller amounts and probably come from contaminations of the serine transfer-RNA fractions. 3 oligonucleotides could not be identified, and one only partially. Several oligonucleotides contain odd nucleotides.
{"title":"Spaltung von löslicher ribonucleinsäure und serin-spezifischen transfer-ribonucleinsäure-fraktionen mit pankreas-ribonuclease","authors":"F. Melchers, H.G. Zachau","doi":"10.1016/0926-6550(64)90003-9","DOIUrl":"10.1016/0926-6550(64)90003-9","url":null,"abstract":"<div><p>Several RNA samples were split with pancreatic RNAase (polyribonucleotide 2-oligonucleotidotransferase (cyclizing), EC 2.7.7.16). The fragments were separated by chromatography on DEAE-cellulose columns and in some cases by subsequent paper electrophoresis. The amounts of mono- and dinucleotides from various unfractionated and partially fractionated soluble RNA's were compared (Table I). In hydrolysates of highly purified serine transfer RNA fractions 7 di-, 7 tri-, 7 tetra-, 2 penta- and 2 hexanucleotides - coming from 2 serine transfer-RNAs - were identified and quantitatively determined (Tables II and III), as well as some oligonucleotides, which occur in smaller amounts and probably come from contaminations of the serine transfer-RNA fractions. 3 oligonucleotides could not be identified, and one only partially. Several oligonucleotides contain odd nucleotides.</p></div>","PeriodicalId":100173,"journal":{"name":"Biochimica et Biophysica Acta (BBA) - Specialized Section on Nucleic Acids and Related Subjects","volume":"91 4","pages":"Pages 559-572"},"PeriodicalIF":0.0,"publicationDate":"1964-12-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0926-6550(64)90003-9","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87173372","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1964-12-16DOI: 10.1016/0926-6550(64)90005-2
R.C. Hignett
1.
1. The effects of 5-fluorouracil, not reversible in the presence of uracil, on Staphylococcus aureus (strain Duncan) were studied.
2.
2. The logarithmic growth of S. aureus (strain Duncan) in the presence of 5-fluorouracil (100 μg/ml) and uracil (200 μg/ml) was retarded by 90% after 60-min incubation.
3.
3. Up to 4% of the UMP in the ribosomal RNA was replaced during 60-min growth, by 5-fluorouracil ribotide: 14% of UMP in the soluble RNA was replaced during this period.
4.
4. The proportions of cell wall, hexose and polysaccharide were unaffected, but the lipid content fell slightly.
5.
5. Total nucleic acid fell by 45% but the total nitrogen (less nucleic acids) remained constant.
6.
6. Continued incorporation of the analogue into soluble RNA was in contrast to its uptake into particulate RNA.
7.
7. Acid-soluble protein from the cell sap showed marked changes in its electrophoretic characteristics.
8.
8. It was concluded that protein synthesis was unabated, with the production of abnormal proteins caused by unnatural nucleic acids; and that ribosome synthesis was halted, although pre-existing ribosomes remained active.
{"title":"The incorporation of 5-fluorouracil by Staphylococcus aureus (strain Duncan). II","authors":"R.C. Hignett","doi":"10.1016/0926-6550(64)90005-2","DOIUrl":"10.1016/0926-6550(64)90005-2","url":null,"abstract":"<div><p></p><ul><li><span>1.</span><span><p>1. The effects of 5-fluorouracil, not reversible in the presence of uracil, on <em>Staphylococcus aureus</em> (strain Duncan) were studied.</p></span></li><li><span>2.</span><span><p>2. The logarithmic growth of <em>S. aureus</em> (strain Duncan) in the presence of 5-fluorouracil (100 μg/ml) and uracil (200 μg/ml) was retarded by 90% after 60-min incubation.</p></span></li><li><span>3.</span><span><p>3. Up to 4% of the UMP in the ribosomal RNA was replaced during 60-min growth, by 5-fluorouracil ribotide: 14% of UMP in the soluble RNA was replaced during this period.</p></span></li><li><span>4.</span><span><p>4. The proportions of cell wall, hexose and polysaccharide were unaffected, but the lipid content fell slightly.</p></span></li><li><span>5.</span><span><p>5. Total nucleic acid fell by 45% but the total nitrogen (less nucleic acids) remained constant.</p></span></li><li><span>6.</span><span><p>6. Continued incorporation of the analogue into soluble RNA was in contrast to its uptake into particulate RNA.</p></span></li><li><span>7.</span><span><p>7. Acid-soluble protein from the cell sap showed marked changes in its electrophoretic characteristics.</p></span></li><li><span>8.</span><span><p>8. It was concluded that protein synthesis was unabated, with the production of abnormal proteins caused by unnatural nucleic acids; and that ribosome synthesis was halted, although pre-existing ribosomes remained active.</p></span></li></ul></div>","PeriodicalId":100173,"journal":{"name":"Biochimica et Biophysica Acta (BBA) - Specialized Section on Nucleic Acids and Related Subjects","volume":"91 4","pages":"Pages 584-591"},"PeriodicalIF":0.0,"publicationDate":"1964-12-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0926-6550(64)90005-2","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"23813907","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1964-12-16DOI: 10.1016/0926-6550(64)90030-1
Giorgio Bernardi
{"title":"Chromatography of nucleoside mono- and polyphosphates on hydroxyapatite","authors":"Giorgio Bernardi","doi":"10.1016/0926-6550(64)90030-1","DOIUrl":"10.1016/0926-6550(64)90030-1","url":null,"abstract":"","PeriodicalId":100173,"journal":{"name":"Biochimica et Biophysica Acta (BBA) - Specialized Section on Nucleic Acids and Related Subjects","volume":"91 4","pages":"Pages 686-688"},"PeriodicalIF":0.0,"publicationDate":"1964-12-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0926-6550(64)90030-1","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"23819458","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1964-12-16DOI: 10.1016/0926-6550(64)90021-0
R.J. Suhadolnik, Joseph G. Cory
{"title":"Further evidence for the biosynthesis of cordycepin and proof of the structure of 3-deoxyribose","authors":"R.J. Suhadolnik, Joseph G. Cory","doi":"10.1016/0926-6550(64)90021-0","DOIUrl":"10.1016/0926-6550(64)90021-0","url":null,"abstract":"","PeriodicalId":100173,"journal":{"name":"Biochimica et Biophysica Acta (BBA) - Specialized Section on Nucleic Acids and Related Subjects","volume":"91 4","pages":"Pages 661-662"},"PeriodicalIF":0.0,"publicationDate":"1964-12-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0926-6550(64)90021-0","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"23813581","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1964-12-16DOI: 10.1016/0926-6550(64)90012-X
Charles Heidelberger, Judith Boohar, G.D. Birnie
{"title":"Fluorinated pyrimidines. XXII. Effects of various compounds on the incorporation of [14C]formate into DNA thymine in suspensions of Ehrlich ascites cells","authors":"Charles Heidelberger, Judith Boohar, G.D. Birnie","doi":"10.1016/0926-6550(64)90012-X","DOIUrl":"10.1016/0926-6550(64)90012-X","url":null,"abstract":"","PeriodicalId":100173,"journal":{"name":"Biochimica et Biophysica Acta (BBA) - Specialized Section on Nucleic Acids and Related Subjects","volume":"91 4","pages":"Pages 636-638"},"PeriodicalIF":0.0,"publicationDate":"1964-12-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0926-6550(64)90012-X","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"23813572","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1964-12-16DOI: 10.1016/0926-6550(64)90018-0
P.S. Sarin, P.C. Zamecnik
{"title":"On the stability of aminoacyl-s-RNA to nucleophilic catalysis","authors":"P.S. Sarin, P.C. Zamecnik","doi":"10.1016/0926-6550(64)90018-0","DOIUrl":"10.1016/0926-6550(64)90018-0","url":null,"abstract":"","PeriodicalId":100173,"journal":{"name":"Biochimica et Biophysica Acta (BBA) - Specialized Section on Nucleic Acids and Related Subjects","volume":"91 4","pages":"Pages 653-655"},"PeriodicalIF":0.0,"publicationDate":"1964-12-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0926-6550(64)90018-0","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"23813578","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1964-12-16DOI: 10.1016/0926-6550(64)90020-9
Joseph A. Ontko, Wells R. Moorehead
{"title":"Histone synthesis after inhibition of deoxyribonucleic acid replication","authors":"Joseph A. Ontko, Wells R. Moorehead","doi":"10.1016/0926-6550(64)90020-9","DOIUrl":"10.1016/0926-6550(64)90020-9","url":null,"abstract":"","PeriodicalId":100173,"journal":{"name":"Biochimica et Biophysica Acta (BBA) - Specialized Section on Nucleic Acids and Related Subjects","volume":"91 4","pages":"Pages 658-660"},"PeriodicalIF":0.0,"publicationDate":"1964-12-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0926-6550(64)90020-9","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"23813580","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1964-12-16DOI: 10.1016/0926-6550(64)90031-3
O.P. Samarina
{"title":"The distribution and properties of cytoplasmic deoxyribonucleic acid-like ribonucleic acid (messenger ribonucleic acid)","authors":"O.P. Samarina","doi":"10.1016/0926-6550(64)90031-3","DOIUrl":"10.1016/0926-6550(64)90031-3","url":null,"abstract":"","PeriodicalId":100173,"journal":{"name":"Biochimica et Biophysica Acta (BBA) - Specialized Section on Nucleic Acids and Related Subjects","volume":"91 4","pages":"Pages 688-691"},"PeriodicalIF":0.0,"publicationDate":"1964-12-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0926-6550(64)90031-3","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"23813590","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1964-12-16DOI: 10.1016/0926-6550(64)90007-6
Theodore C. Hsiao
Ribosomes were isolated from root tips of corn (Zea mays) and their properties studied. Five ribosomal components (40 S, 60 S, 80 S, 120 S, and 150 S), with the 80-S component predominating, were present in the cytoplasmic extract. Sedimentation coefficients at infinite dilution were determined for all except the 150-S component.
Purified ribosomes contained 43% RNA which was characterized by a high guanine and relatively high cytosine content. The 80-S component dissociated into 60-S and 40-S particles when pH of the medium was increased from 6.5 to 8.0 or higher. Magnesium (0.5–2 mM) prevented the dissociation.
In marked contrast to the 70-S ribosome of Escherichia coli, the 80-S ribosome of corn did not require added magnesium to maintain its integrity in a solution of nearly neutral pH and low ionic strength. In fact, 5 mM magnesium in such a solution caused the association of corn ribosomes into large random aggregates. Ribosomes of different organisms differ in the level of magnesium required to preserve their 70–80-S component and may be divided accordingly into three broad groups exemplified by E. coli, yeast, and corn ribosomes.
{"title":"Characteristics of ribosomes isolated from roots of Zea mays","authors":"Theodore C. Hsiao","doi":"10.1016/0926-6550(64)90007-6","DOIUrl":"10.1016/0926-6550(64)90007-6","url":null,"abstract":"<div><p>Ribosomes were isolated from root tips of corn (<em>Zea mays</em>) and their properties studied. Five ribosomal components (40 S, 60 S, 80 S, 120 S, and 150 S), with the 80-S component predominating, were present in the cytoplasmic extract. Sedimentation coefficients at infinite dilution were determined for all except the 150-S component.</p><p>Purified ribosomes contained 43% RNA which was characterized by a high guanine and relatively high cytosine content. The 80-S component dissociated into 60-S and 40-S particles when pH of the medium was increased from 6.5 to 8.0 or higher. Magnesium (0.5–2 mM) prevented the dissociation.</p><p>In marked contrast to the 70-S ribosome of <em>Escherichia coli</em>, the 80-S ribosome of corn did not require added magnesium to maintain its integrity in a solution of nearly neutral pH and low ionic strength. In fact, 5 mM magnesium in such a solution caused the association of corn ribosomes into large random aggregates. Ribosomes of different organisms differ in the level of magnesium required to preserve their 70–80-S component and may be divided accordingly into three broad groups exemplified by <em>E. coli</em>, yeast, and corn ribosomes.</p></div>","PeriodicalId":100173,"journal":{"name":"Biochimica et Biophysica Acta (BBA) - Specialized Section on Nucleic Acids and Related Subjects","volume":"91 4","pages":"Pages 598-605"},"PeriodicalIF":0.0,"publicationDate":"1964-12-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0926-6550(64)90007-6","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"23813909","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1964-12-16DOI: 10.1016/0926-6550(64)90013-1
Charles Heidelberger, Judith Boohar
{"title":"Fluorinated pyrimidines. XXIII. Further studies on nucleoside phosphorylase","authors":"Charles Heidelberger, Judith Boohar","doi":"10.1016/0926-6550(64)90013-1","DOIUrl":"10.1016/0926-6550(64)90013-1","url":null,"abstract":"","PeriodicalId":100173,"journal":{"name":"Biochimica et Biophysica Acta (BBA) - Specialized Section on Nucleic Acids and Related Subjects","volume":"91 4","pages":"Pages 639-641"},"PeriodicalIF":0.0,"publicationDate":"1964-12-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0926-6550(64)90013-1","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"23813573","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
扫码关注我们
求助内容：
应助结果提醒方式：
京公网安备 11010802042870号