Biomarkers and Genomic Medicine最新文献

Atherosclerosis is a chronic inflammatory vascular disease, and an association between elevated blood lipids and atherosclerosis has previously been recognized. Low-density lipoprotein cholesterol (LDLc) levels have also been recognized as indicators of cardiovascular complications, but two people who have exactly the same LDLc concentrations may have different risk factors, and the National Cholesterol Education Program Adult Treatment Panel III (NCEP ATP III) guidelines recommend patching non-high-density lipoprotein cholesterol (non-HDLc) as another indicator. The present study investigated diabetic dyslipidemia to determine if there is a correlation between dyslipidemia and C-reactive protein (CRP). The data shows that non-HDLc was an independent risk factor for cardiovascular events in patients with diabetes and that CRP could indicate the risk of future cardiovascular events in both high-risk and healthy individuals.

Congenital ichthyosis is a type of generalized hyperkeratinization of the skin at birth. Three forms of congenital ichthyosis have been defined based on clinical aspects and severity: (1) congenital ichthyosis mitis; (2) congenital ichthyosis tarda; and (3) congenital ichthyosis gravis. Desquamation of the parchment-like hyperkeratinized skin begins shortly after birth and may require several weeks to complete. Skin alterations in the eyelid cause shortening of the anterior lamella, subsequently resulting in ectropion. This affects the upper eyelid more often than the lower eyelid and can lead to complications such as chronic palpebral or bulbar conjunctivitis and keratinization or exposure keratopathy. In this paper, we present two case reports illustrating the course of ichthyosis congenita mitis and ichthyosis congenita tarda.

The objective of this study is to explore the relationship between migration ability and invadosome organization in human oral squamous cancer cells. Wound healing assay, immunofluorescence, Western blotting, and matrix metalloproteinase zymogen gel assay were utilized to investigate the phenotype changes of three oral squamous cancer cells (OC3, OC3-I5, and OC3-IV2). Among these three cell lines, selective subpopulations of OC3, OC3-I5, and OC3-IV2 have high migratory ability compared to OC3 in a wound healing assay. Moreover, immunofluorescence showed that formation of actin dots and paxillin rings in OC3 was less than in OC3-I5 and OC3-IV2. Minor differences of other adhesion molecules and matrix metalloproteinase activity have also been observed. Enhanced cell migration ability and increased invadosomes have been detected in both in vitro selection cell line OC3-I5 and in vivo selection cell line OC3-IV2, suggesting that there may be a correlation between migration activity and invadosome formation in invasive oral cancer cells. Therefore, it would be interesting for us to continue discovering more invadosome components and evaluate whether they play any role in the regulation of the invasion ability of oral cancer cells.

Cancer is the leading cause of death in the world, and therefore, identifying advanced anticancer drugs is the most important objective of cancer research. However, at present, most synthetic anticancer drugs developed cause serious side effects thereby reducing their therapeutic efficiency. In a previous study, we have reported that culture filtrates from Barnettozyma spp. can not only maintain their lethal effect under physical environment, but can also exert stronger cytotoxicity to the hepatoma cell line HepG2 than to the normal Chang's liver cell. Herein, we further classify the types of liver cancer cell death, and identify those proteins responsible for the cell death by matrix-assisted laser desorption/ionization–time-of-flight spectra to study the possible mechanism of lethal culture filtrate from a special Taiwan killer yeast Barnettozyma spp. (EN14S14). Results of our preliminary study indicated that autophagic induction is most likely the major form of HepG2 cell death, which is induced by the “killing” culture filtrate. Proteins differentially expressed after treatment fell into three major categories, namely, metabolism, cytoskeleton, and signal transduction.

Hepatocellular carcinoma (HCC) is the most common malignant liver tumor. The purpose of this study is to characterize proteins secreted from the HepG2 cells, which may relate to cell differentiation and tumor metastasis. In the proteomic analysis, the secretome was identified by nano-high–performance liquid chromatography/electrospray ionization tandem mass spectrometry (nano-HPLC/ESIMS/MS) followed by peptide fragmentation pattern analysis. In this study, three proteins, p130Cas-associated protein (p130Cas/BCAR1), TAR DNA-binding protein 43 (TDP43/TARDBP) and translationally controlled tumor protein (TCTP/TPT1), were identified and confirmed by Western blotting, which showed significantly differential expression compared with the normal liver cells. Analyzing differential protein expressions in HepG2 cell by proteomic approaches suggests that p130Cas/BCAR1, TDP43/TARDBP and TCTP/TPT1 as key proteins and may serve as biomarkers for HCC.