Pub Date : 2020-01-28DOI: 10.3760/CMA.J.ISSN.1007-8118.2020.01.001
Jia-Ming Liu, Wei Liu, Da Xu, Li-Jun Wang, Kun Wang, B. Xing
Objective To study the impact of RAS status on prognosis of patients after liver resection for colorectal cancer liver metastases (CRLM). Methods The data of 545 consecutive CRLM patients who underwent liver resection at the Hepatopancreatobiliary Surgery Department I, Peking University Cancer Hospital between January 1st, 2008 and December 31st, 2016, were retrospectively reviewed. According to the inclusion and exclusion criteria, 356 patients were eventually included into this study. There were 232 males and 124 females, with ages ranging from 21 to 83 years. The clinical and follow-up data of patients with wild-type and mutant RAS were compared. Survival was estimated by the Kaplan-Meier method, and the difference was compared by the log-rank test. Factors influencing survival of these patients were assessed by univariate and multivariate Cox regression analyses. Results There were 247 patients with wild-type RAS and 109 patients with mutant RAS, respectively. The median overall survival of patients with wild-type and mutant RAS were 74 and 30 months respectively. Compared with mutant RAS patients, wild-type RAS patients had significantly better cumulative survival and disease free survival rates (both P 5 cm (HR=1.717, 95%CI: 1.102-2.637), and mutant RAS (HR=1.836, 95%CI: 1.322-2.550) were independent risk factors for patients with colorectal cancer liver metastases after hepatic resection. Conclusion Mutant RAS was a poor prognostic factor of survival after liver resection in CRLM patients Key words: Colorectal neoplasms; Hepatectomy; Liver metastases; RAS gene
{"title":"Impact of mutant RAS on prognosis of patients after hepatic resection for colorectal cancer liver metastases","authors":"Jia-Ming Liu, Wei Liu, Da Xu, Li-Jun Wang, Kun Wang, B. Xing","doi":"10.3760/CMA.J.ISSN.1007-8118.2020.01.001","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.1007-8118.2020.01.001","url":null,"abstract":"Objective \u0000To study the impact of RAS status on prognosis of patients after liver resection for colorectal cancer liver metastases (CRLM). \u0000 \u0000 \u0000Methods \u0000The data of 545 consecutive CRLM patients who underwent liver resection at the Hepatopancreatobiliary Surgery Department I, Peking University Cancer Hospital between January 1st, 2008 and December 31st, 2016, were retrospectively reviewed. According to the inclusion and exclusion criteria, 356 patients were eventually included into this study. There were 232 males and 124 females, with ages ranging from 21 to 83 years. The clinical and follow-up data of patients with wild-type and mutant RAS were compared. Survival was estimated by the Kaplan-Meier method, and the difference was compared by the log-rank test. Factors influencing survival of these patients were assessed by univariate and multivariate Cox regression analyses. \u0000 \u0000 \u0000Results \u0000There were 247 patients with wild-type RAS and 109 patients with mutant RAS, respectively. The median overall survival of patients with wild-type and mutant RAS were 74 and 30 months respectively. Compared with mutant RAS patients, wild-type RAS patients had significantly better cumulative survival and disease free survival rates (both P 5 cm (HR=1.717, 95%CI: 1.102-2.637), and mutant RAS (HR=1.836, 95%CI: 1.322-2.550) were independent risk factors for patients with colorectal cancer liver metastases after hepatic resection. \u0000 \u0000 \u0000Conclusion \u0000Mutant RAS was a poor prognostic factor of survival after liver resection in CRLM patients \u0000 \u0000 \u0000Key words: \u0000Colorectal neoplasms; Hepatectomy; Liver metastases; RAS gene","PeriodicalId":10021,"journal":{"name":"Chinese Journal of Hepatobiliary Surgery","volume":"26 1","pages":"1-5"},"PeriodicalIF":0.0,"publicationDate":"2020-01-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45285724","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Left hepatectomy of hepatocellular carcinoma at Barcelona Clinic Liver Cancer staged C after downgrading transformation with the combination of targeted and immunotherapy: a case report","authors":"Bingyang Hu, Wen-wen Zhang, Qianchuan Zhou, Shi-xin Lu","doi":"10.3760/CMA.J.ISSN.1007-8118.2020.01.016","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.1007-8118.2020.01.016","url":null,"abstract":"患者男性，54岁，诊断为肝左叶肝癌并肝内多发转移伴门静脉主干及左支癌栓(巴塞罗那分期为C期，门静脉癌栓Ⅲ型)。该患者手术不可切除，也不符合经导管肝动脉栓塞化疗适应证，故选择抗血管生成靶向药物阿帕替尼联合程序性死亡蛋白-1抗体治疗。经过6个周期治疗，肝左叶主病灶明显缩小，门静脉癌栓从门静脉主干退至门静脉左支内，肝内转移病灶消失，经评估后成功施行了荧光腹腔镜左半肝切除术。","PeriodicalId":10021,"journal":{"name":"Chinese Journal of Hepatobiliary Surgery","volume":"26 1","pages":"65-67"},"PeriodicalIF":0.0,"publicationDate":"2020-01-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46890551","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-01-28DOI: 10.3760/CMA.J.ISSN.1007-8118.2020.01.012
Ying Ding, Li Yang, Wen-tao Wang, M. Zeng, Sheng-xiang Rao
Objective To investigate the value of gadoxetic acid-enhanced magnetic resonance imaging (MRI) on oxaliplatin-induced liver function injury in C57BL/6 mice. Methods Forty male and six weeks old C57BL/6 mice without specific pathogens were included and the body weght ranged from 19 to 23 g. They were randomly assigned into control group, experimental group A, experimental group B and experimental group C (10 mice/group). The mice in the control group was intraperitoneally injected with saline solution. The mice in the experimental groups were intraperitoneally injected with oxaliplatin twice a week. The experimental group A, B and C were administered for 2 weeks, 4 weeks and 6 weeks, respectively. The T1 relaxation time on the hepatobiliary phase and the first rapid enhancement slope percentage (ESP) in liver parenchyma were measured and calculated. Serum albumin and bilirubin values were measured and albumin and bilirubin (ALBI) scores were calculated. Pathological staining was used to observe liver tissue damage and fibrosis. The receiver operating characteristic (ROC) curve evaluated the ALBI score, ESP and T1 relaxation time on hepatobiliary phase for the diagnosis of liver function. Results Sixteen mice in the experimental groups (including group A, B and C) were included in the hepatic degeneration group (hepatocyte degeneration without fibrosis). Fourteen mice were included in the hepatic fibrosis group. T1 relaxation time on hepatobiliary phase in the hepatic fibrosis group was higher than that in the control group and in the hepatic degeneration group. The differences were statistically significant (P<0.05). The ESP of the control group, the hepatic degeneration group and the hepatic fibrosis group was increased, with statistically significant differences (all P<0.05). Compared with the control group, ALBI scores of the hepatic degeneration group and the hepatic fibrosis group were both decreased, with statistically significant differences (P<0.05). In the hepatic fibrosis group, the areas under the curve of ALBI scores, the T1 relaxation time on hepatobiliary phase and the ESP were 0.734, 0.962 and 0.989, respectively. Conclusion The T1 relaxation time on hepatobiliary phase and the ESP of gadoxetic acid-enhanced MRI can effectively evaluate the hepatic function reduction induced by oxaliplatin-induced hepatic tissue injury in C57BL/6 mice. Key words: Magnetic resonance imaging; Gadoxetic acid; Albumin-bilirubin; Liver function
{"title":"Value of the gadoxetic acid-enhanced magnetic resonance imaging on oxaliplatin-induced liver function injury in C57BL/6 mice","authors":"Ying Ding, Li Yang, Wen-tao Wang, M. Zeng, Sheng-xiang Rao","doi":"10.3760/CMA.J.ISSN.1007-8118.2020.01.012","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.1007-8118.2020.01.012","url":null,"abstract":"Objective \u0000To investigate the value of gadoxetic acid-enhanced magnetic resonance imaging (MRI) on oxaliplatin-induced liver function injury in C57BL/6 mice. \u0000 \u0000 \u0000Methods \u0000Forty male and six weeks old C57BL/6 mice without specific pathogens were included and the body weght ranged from 19 to 23 g. They were randomly assigned into control group, experimental group A, experimental group B and experimental group C (10 mice/group). The mice in the control group was intraperitoneally injected with saline solution. The mice in the experimental groups were intraperitoneally injected with oxaliplatin twice a week. The experimental group A, B and C were administered for 2 weeks, 4 weeks and 6 weeks, respectively. The T1 relaxation time on the hepatobiliary phase and the first rapid enhancement slope percentage (ESP) in liver parenchyma were measured and calculated. Serum albumin and bilirubin values were measured and albumin and bilirubin (ALBI) scores were calculated. Pathological staining was used to observe liver tissue damage and fibrosis. The receiver operating characteristic (ROC) curve evaluated the ALBI score, ESP and T1 relaxation time on hepatobiliary phase for the diagnosis of liver function. \u0000 \u0000 \u0000Results \u0000Sixteen mice in the experimental groups (including group A, B and C) were included in the hepatic degeneration group (hepatocyte degeneration without fibrosis). Fourteen mice were included in the hepatic fibrosis group. T1 relaxation time on hepatobiliary phase in the hepatic fibrosis group was higher than that in the control group and in the hepatic degeneration group. The differences were statistically significant (P<0.05). The ESP of the control group, the hepatic degeneration group and the hepatic fibrosis group was increased, with statistically significant differences (all P<0.05). Compared with the control group, ALBI scores of the hepatic degeneration group and the hepatic fibrosis group were both decreased, with statistically significant differences (P<0.05). In the hepatic fibrosis group, the areas under the curve of ALBI scores, the T1 relaxation time on hepatobiliary phase and the ESP were 0.734, 0.962 and 0.989, respectively. \u0000 \u0000 \u0000Conclusion \u0000The T1 relaxation time on hepatobiliary phase and the ESP of gadoxetic acid-enhanced MRI can effectively evaluate the hepatic function reduction induced by oxaliplatin-induced hepatic tissue injury in C57BL/6 mice. \u0000 \u0000 \u0000Key words: \u0000Magnetic resonance imaging; Gadoxetic acid; Albumin-bilirubin; Liver function","PeriodicalId":10021,"journal":{"name":"Chinese Journal of Hepatobiliary Surgery","volume":"26 1","pages":"53-56"},"PeriodicalIF":0.0,"publicationDate":"2020-01-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41624739","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-01-28DOI: 10.3760/CMA.J.ISSN.1007-8118.2020.01.011
Minghui Zhu, Xianzhou Zhang, Feng Han, Changfu Nie
Objective To study factors affecting prognosis of patients with intrahepatic cholangiocarcinoma (ICC), focusing on the correlation between extent of lymph node dissection and prognosis of patients with ICC. Methods The clinical data of ICC patients who underwent radical resection at the Hepatobiliary and Pancreatic Surgery of Affiliated Cancer Hospital of Zhengzhou University from October 2013 to October 2017 were retrospectively analyzed. According to the extent of lymph node dissection, the patients were divided into the non-dissected lymph node group, the routine dissection lymph node group and the extended lymph node dissection group. The prognoses of the three groups were compared. The Cox stepwise regression model was used to analyze the independent risk factors for prognosis of patients with ICC. Results The 178 patients included 109 males and 69 females. Their ages ranged from 30 to 81 years (average 59 years). There were 80 patients in the non-dissected group, 34 patients in the routine lymph node dissection group, and 64 patients in the extended lymph node dissection group. The overall survival rates of the 178 patients at 3 years after liver resection was 29.2%(52/178), overall median survival 25.8 months. The 3-year survival rates of the non-dissected group, routine dissection group, and extended dissection group were 10.0%(8/80), 52.9%(18/34), 40.6%(26/64), respectively. The differences among the three groups were significant (P 0.05). There was a significant difference in survival rates between the non-dissected group and the extended lymph node dissection group (P<0.05). Univariate analysis showed that CA19-9, tumor diameter, portal tumor thrombus, and lymph node dissection were related to prognosis of patients with ICC (P<0.05). Multivariate analysis showed CA19-9, tumor diameter, and extent of lymph nodes clearance were related to patient survival (P<0.05). Conclusions CA19-9, tumor diameter, and extent of lymph node dissection were independent risk factors of survival in patients with ICC. For patients with ICC who undergo surgical resection, conventional laparoscopic lymph node dissection can achieve good results, and there is no need to extend lymph node dissection. Key words: Cholangiocarcinoma; Lymph node excision; Prognosis analysis
{"title":"Prognostic factors of radical resection on patients with intrahepatic cholangiocarcinoma and impact of abdominal lymph node dissection on prognosis","authors":"Minghui Zhu, Xianzhou Zhang, Feng Han, Changfu Nie","doi":"10.3760/CMA.J.ISSN.1007-8118.2020.01.011","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.1007-8118.2020.01.011","url":null,"abstract":"Objective \u0000To study factors affecting prognosis of patients with intrahepatic cholangiocarcinoma (ICC), focusing on the correlation between extent of lymph node dissection and prognosis of patients with ICC. \u0000 \u0000 \u0000Methods \u0000The clinical data of ICC patients who underwent radical resection at the Hepatobiliary and Pancreatic Surgery of Affiliated Cancer Hospital of Zhengzhou University from October 2013 to October 2017 were retrospectively analyzed. According to the extent of lymph node dissection, the patients were divided into the non-dissected lymph node group, the routine dissection lymph node group and the extended lymph node dissection group. The prognoses of the three groups were compared. The Cox stepwise regression model was used to analyze the independent risk factors for prognosis of patients with ICC. \u0000 \u0000 \u0000Results \u0000The 178 patients included 109 males and 69 females. Their ages ranged from 30 to 81 years (average 59 years). There were 80 patients in the non-dissected group, 34 patients in the routine lymph node dissection group, and 64 patients in the extended lymph node dissection group. The overall survival rates of the 178 patients at 3 years after liver resection was 29.2%(52/178), overall median survival 25.8 months. The 3-year survival rates of the non-dissected group, routine dissection group, and extended dissection group were 10.0%(8/80), 52.9%(18/34), 40.6%(26/64), respectively. The differences among the three groups were significant (P 0.05). There was a significant difference in survival rates between the non-dissected group and the extended lymph node dissection group (P<0.05). Univariate analysis showed that CA19-9, tumor diameter, portal tumor thrombus, and lymph node dissection were related to prognosis of patients with ICC (P<0.05). Multivariate analysis showed CA19-9, tumor diameter, and extent of lymph nodes clearance were related to patient survival (P<0.05). \u0000 \u0000 \u0000Conclusions \u0000CA19-9, tumor diameter, and extent of lymph node dissection were independent risk factors of survival in patients with ICC. For patients with ICC who undergo surgical resection, conventional laparoscopic lymph node dissection can achieve good results, and there is no need to extend lymph node dissection. \u0000 \u0000 \u0000Key words: \u0000Cholangiocarcinoma; Lymph node excision; Prognosis analysis","PeriodicalId":10021,"journal":{"name":"Chinese Journal of Hepatobiliary Surgery","volume":"26 1","pages":"48-52"},"PeriodicalIF":0.0,"publicationDate":"2020-01-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43520891","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-01-28DOI: 10.3760/CMA.J.ISSN.1007-8118.2020.01.010
Guoming Li, Wen-bing Sun, S. Ke, Shigang Guo
Objective To study the value of radiofrequency ablation of gallbladder beds in radical surgery for stage T1b gallbladder cancer. Methods A retrospective study was conducted on 21 patients with stage T1b gallbladder cancer treated in the Department of Hepatobiliary Pancreas and Spleen Surgery, West Campus, Beijing Chaoyang Hospital, Capital Medical University, from April 1, 2011 to March 31, 2019. Patients who were diagnosed with frozen section during operation were included into the surgery group. Patients who were diagnosed postoperatively and underwent radical surgery for the gallbladder carcinoma were included in the secondary surgery group. The differences in surgical time, bleeding volume, survival rate, and survival time between the two groups after regular follow-up were compared, and the 5-year cumulative survival rates and overall survival time of the two groups were calculated. Results All patients underwent radical gallbladder cancer surgery. There were 14 males and 7 females, aged from 26 to 70 (49.0±13.5) years. There were no perioperative deaths. There were 15 patients in the operation group, and 6 patients in the secondary surgery group. The bleeding volume of the surgery group was significantly less than the secondary surgery group [(101.3±35.5) ml vs. (177.0±44.6) ml, P 0.05). The 5-year cumulative survival rate for the two groups of patients was 56.5%, and the overall survival time was (79.0±9.3) months. Conclusions Radiofrequency ablation of the gallbladder bed was safe and effective in radical surgery of T1b gallbladder cancer. For T1b stage gallbladder cancer, which is difficult to diagnose during surgery, secondary radical surgery achieved the same results as primary radical surgery. Key words: Gallbladder neoplasms; Radiofrequency ablation; T1b stage; Gallbladder bed
目的探讨胆囊床射频消融在T1b期胆囊癌根治术中的应用价值。方法回顾性分析2011年4月1日至2019年3月31日在首都医科大学北京朝阳医院西校区肝胆胰脾外科治疗的21例T1b期胆囊癌患者的临床资料。术中诊断为冰冻切片者为手术组。术后确诊并行根治性胆囊癌的患者为二次手术组。比较两组定期随访后手术时间、出血量、生存率、生存时间的差异，计算两组5年累计生存率和总生存时间。结果所有患者均行根治性胆囊癌手术。男性14例，女性7例，年龄26 ~ 70岁(49.0±13.5)岁。无围手术期死亡。手术组15例，二次手术组6例。手术组出血量明显少于二次手术组[(101.3±35.5)ml vs(177.0±44.6)ml, P < 0.05]。两组患者5年累计生存率为56.5%，总生存时间为(79.0±9.3)个月。结论胆囊床射频消融术在T1b胆囊癌根治术中安全有效。对于手术中难以诊断的T1b期胆囊癌，继发根治术与原发性根治术效果相同。关键词:胆囊肿瘤;射频消融术;T1b阶段;胆囊床
{"title":"Radiofrequency ablation of gallbladder bed in radical surgery for stage T1b gallbladder cancer","authors":"Guoming Li, Wen-bing Sun, S. Ke, Shigang Guo","doi":"10.3760/CMA.J.ISSN.1007-8118.2020.01.010","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.1007-8118.2020.01.010","url":null,"abstract":"Objective \u0000To study the value of radiofrequency ablation of gallbladder beds in radical surgery for stage T1b gallbladder cancer. \u0000 \u0000 \u0000Methods \u0000A retrospective study was conducted on 21 patients with stage T1b gallbladder cancer treated in the Department of Hepatobiliary Pancreas and Spleen Surgery, West Campus, Beijing Chaoyang Hospital, Capital Medical University, from April 1, 2011 to March 31, 2019. Patients who were diagnosed with frozen section during operation were included into the surgery group. Patients who were diagnosed postoperatively and underwent radical surgery for the gallbladder carcinoma were included in the secondary surgery group. The differences in surgical time, bleeding volume, survival rate, and survival time between the two groups after regular follow-up were compared, and the 5-year cumulative survival rates and overall survival time of the two groups were calculated. \u0000 \u0000 \u0000Results \u0000All patients underwent radical gallbladder cancer surgery. There were 14 males and 7 females, aged from 26 to 70 (49.0±13.5) years. There were no perioperative deaths. There were 15 patients in the operation group, and 6 patients in the secondary surgery group. The bleeding volume of the surgery group was significantly less than the secondary surgery group [(101.3±35.5) ml vs. (177.0±44.6) ml, P 0.05). The 5-year cumulative survival rate for the two groups of patients was 56.5%, and the overall survival time was (79.0±9.3) months. \u0000 \u0000 \u0000Conclusions \u0000Radiofrequency ablation of the gallbladder bed was safe and effective in radical surgery of T1b gallbladder cancer. For T1b stage gallbladder cancer, which is difficult to diagnose during surgery, secondary radical surgery achieved the same results as primary radical surgery. \u0000 \u0000 \u0000Key words: \u0000Gallbladder neoplasms; Radiofrequency ablation; T1b stage; Gallbladder bed","PeriodicalId":10021,"journal":{"name":"Chinese Journal of Hepatobiliary Surgery","volume":"26 1","pages":"44-47"},"PeriodicalIF":0.0,"publicationDate":"2020-01-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43735276","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-01-28DOI: 10.3760/CMA.J.ISSN.1007-8118.2020.01.008
Rong-kun Li, Ya-chuan Cao, Yue-chun Shen, Jun Li
Objective To study the correlations between tumor mutation burden (TMB) and the prognosis of hepatocellular carcinoma (HCC) patients, and to investigate the effect of TMB on differential expression genes of HCC and the proportion of invasive immune cells in tumor tissues. Methods The somatic variation data, gene transcriptional expression data and clinical information of HCC patients were obtained from the cancer genome atlas (TCGA) database. The R program language (version 3.6.1) maftools function package was used to analyze the gene mutation data characteristics of the samples. The TMB value of each sample was calculated using the full-exon sequencing data of patients with hepatocellular carcinoma on the VarScan2 platform, sorted by TMB value, and the median value was used to divide all samples into high TMB and low TMB groups. Kaplan-Meier method was used to draw the survival curves of two groups of patients and log-rank test was performed to determine the correlation between tumor mutation load and prognosis. The Limma function package of R language was used to screen the differentially expressed genes between the two groups (FDR=0.05 and logFC=1), and the clusterProfiler function package of R language was used to perform gene ontology (GO) enrichment analysis of the differential genes and kyoto encyclopedia of genes and genomes (KEGG) enrichment analysis (screening criteria were all P<0.05). Then the CIBERSORT tool was used to compare and analyze the difference in the proportion of invasive immune cells between the two groups. Results A total of 364 patients with HCC from TCGA database were included in the study. Mutations were found in 327(84%) samples, and there was a synergistic correlation between OBSCN and FLG mutations (P 0.05). A total of 198 with differentially expressed genes (28 up-regulated genes and 170 down-regulated genes) were screened between the high TMB group and the low TMB group. In GO analysis, it was found that the differentially expressed genes were mainly enriched in extracellular matrix tissues, extracellular structural tissues, extracellular matrix, extracellular matrix containing collagen, extracellular matrix structural components and other functions. In KEGG analysis, differential genes were highly enriched in extracellular matrix receptor interaction pathway and adhesive plaque pathway. In the correlation analysis of the proportion of infiltrating immune cells, CD4+ memory T cells were more infiltrating in the low TMB group (P<0.05). Monocytes showed a higher degree of infiltration in the high TMB group (P<0.05). Conclusion There was no correlation between TMB and the prognosis of HCC patients. TMB has significant influence on the differential expression genes of HCC and the proportion of invasive immune cells in tumor tissues. Key words: Carcinoma, hepatocellular; Tumor mutation burden; Prognosis
{"title":"Correlations analysis between HCC mutation burden and patients’ prognosis based on data mining","authors":"Rong-kun Li, Ya-chuan Cao, Yue-chun Shen, Jun Li","doi":"10.3760/CMA.J.ISSN.1007-8118.2020.01.008","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.1007-8118.2020.01.008","url":null,"abstract":"Objective \u0000To study the correlations between tumor mutation burden (TMB) and the prognosis of hepatocellular carcinoma (HCC) patients, and to investigate the effect of TMB on differential expression genes of HCC and the proportion of invasive immune cells in tumor tissues. \u0000 \u0000 \u0000Methods \u0000The somatic variation data, gene transcriptional expression data and clinical information of HCC patients were obtained from the cancer genome atlas (TCGA) database. The R program language (version 3.6.1) maftools function package was used to analyze the gene mutation data characteristics of the samples. The TMB value of each sample was calculated using the full-exon sequencing data of patients with hepatocellular carcinoma on the VarScan2 platform, sorted by TMB value, and the median value was used to divide all samples into high TMB and low TMB groups. Kaplan-Meier method was used to draw the survival curves of two groups of patients and log-rank test was performed to determine the correlation between tumor mutation load and prognosis. The Limma function package of R language was used to screen the differentially expressed genes between the two groups (FDR=0.05 and logFC=1), and the clusterProfiler function package of R language was used to perform gene ontology (GO) enrichment analysis of the differential genes and kyoto encyclopedia of genes and genomes (KEGG) enrichment analysis (screening criteria were all P<0.05). Then the CIBERSORT tool was used to compare and analyze the difference in the proportion of invasive immune cells between the two groups. \u0000 \u0000 \u0000Results \u0000A total of 364 patients with HCC from TCGA database were included in the study. Mutations were found in 327(84%) samples, and there was a synergistic correlation between OBSCN and FLG mutations (P 0.05). A total of 198 with differentially expressed genes (28 up-regulated genes and 170 down-regulated genes) were screened between the high TMB group and the low TMB group. In GO analysis, it was found that the differentially expressed genes were mainly enriched in extracellular matrix tissues, extracellular structural tissues, extracellular matrix, extracellular matrix containing collagen, extracellular matrix structural components and other functions. In KEGG analysis, differential genes were highly enriched in extracellular matrix receptor interaction pathway and adhesive plaque pathway. In the correlation analysis of the proportion of infiltrating immune cells, CD4+ memory T cells were more infiltrating in the low TMB group (P<0.05). Monocytes showed a higher degree of infiltration in the high TMB group (P<0.05). \u0000 \u0000 \u0000Conclusion \u0000There was no correlation between TMB and the prognosis of HCC patients. TMB has significant influence on the differential expression genes of HCC and the proportion of invasive immune cells in tumor tissues. \u0000 \u0000 \u0000Key words: \u0000Carcinoma, hepatocellular; Tumor mutation burden; Prognosis","PeriodicalId":10021,"journal":{"name":"Chinese Journal of Hepatobiliary Surgery","volume":"26 1","pages":"32-37"},"PeriodicalIF":0.0,"publicationDate":"2020-01-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44436696","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Objective To study the impact of adjuvant transarterial chemoembolization (TACE) after curative resection for patients with hepatocellular carcinoma (HCC) and microvascular invasion (MVI). Methods PubMed, Cochrane library, Embase, Web of Science, CNKI, VIP, Wanfang were searched for randomized or non-randomized studies which compared recurrence-free survival and overall survival rates with or without postoperative adjuvant TACE after curative resection for patients with HCC and MVI. The limited search time was from January 1st, 1999 to May 1st, 2019. Statistical analyses were performed with software programs using Stata (version 15.0) and Review Manage (version 5.3). Results Eight studies which included 1 809 patients were studied. There were 815 patients who received and 994 patients who did not receive postoperative adjuvant TACE after radical hepatectomy. Postoperative adjuvant TACE significantly improved recurrence-free survival and overall survival rates in patients with HCC and MVI when compared with the patients who required no adjuvant TACE (HR=0.74, 95%CI: 0.65-0.85, P 5 cm benefited from postoperative adjuvant TACE only in recurrence-free survival rate (HR=0.72, 95%CI: 0.58-0.90, P<0.05), but not in overall survival rate. Conclusion Postoperative adjuvant TACE delayed recurrence of microvascular invasion of HCC and improved long-term prognosis, but not for tumors with diameter >5 cm. These results need to be validated in further clinical trials. Key words: Carcinoma, hepatocellular; Hepatectomy; Transcatheter arterial chemoembolization; Microvascular invasion; Prognosis
目的探讨肝细胞癌（HCC）及微血管侵犯（MVI）根治性切除术后辅助动脉化疗栓塞（TACE）的疗效。方法检索PubMed、Cochrane library、Embase、Web of Science、CNKI、VIP、万方等随机或非随机研究，比较HCC和MVI患者根治性切除术后使用或不使用术后辅助TACE的无复发生存率和总生存率。有限搜索时间为1999年1月1日至2019年5月1日。使用Stata（15.0版）和Review Manage（5.3版）软件进行统计分析。结果研究了8项研究，包括1809名患者。有815名患者接受了根治性肝切除术，994名患者没有接受术后辅助TACE。与不需要辅助TACE的患者相比，术后辅助TACE显著提高了HCC和MVI患者的无复发生存率和总生存率（HR=0.74，95%CI:0.65-0.85，P5cm仅在无复发存活率方面受益于术后辅助性TACE（HR=0.72，95%CI:0.58-0.90，P5cm）。这些结果需要在进一步的临床试验中得到验证。关键词：肝癌；肝切除术；经导管动脉化疗栓塞；微血管侵犯；预后
{"title":"Adjuvant transarterial chemoembolization after curative resection for hepatocellular carcinoma with microvascular invasion: a meta-analysis","authors":"Jian Zhao, Jianbo Xu, Zhiying Gao, Jian-huai Zhang","doi":"10.3760/CMA.J.ISSN.1007-8118.2020.01.009","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.1007-8118.2020.01.009","url":null,"abstract":"Objective \u0000To study the impact of adjuvant transarterial chemoembolization (TACE) after curative resection for patients with hepatocellular carcinoma (HCC) and microvascular invasion (MVI). \u0000 \u0000 \u0000Methods \u0000PubMed, Cochrane library, Embase, Web of Science, CNKI, VIP, Wanfang were searched for randomized or non-randomized studies which compared recurrence-free survival and overall survival rates with or without postoperative adjuvant TACE after curative resection for patients with HCC and MVI. The limited search time was from January 1st, 1999 to May 1st, 2019. Statistical analyses were performed with software programs using Stata (version 15.0) and Review Manage (version 5.3). \u0000 \u0000 \u0000Results \u0000Eight studies which included 1 809 patients were studied. There were 815 patients who received and 994 patients who did not receive postoperative adjuvant TACE after radical hepatectomy. Postoperative adjuvant TACE significantly improved recurrence-free survival and overall survival rates in patients with HCC and MVI when compared with the patients who required no adjuvant TACE (HR=0.74, 95%CI: 0.65-0.85, P 5 cm benefited from postoperative adjuvant TACE only in recurrence-free survival rate (HR=0.72, 95%CI: 0.58-0.90, P<0.05), but not in overall survival rate. \u0000 \u0000 \u0000Conclusion \u0000Postoperative adjuvant TACE delayed recurrence of microvascular invasion of HCC and improved long-term prognosis, but not for tumors with diameter >5 cm. These results need to be validated in further clinical trials. \u0000 \u0000 \u0000Key words: \u0000Carcinoma, hepatocellular; Hepatectomy; Transcatheter arterial chemoembolization; Microvascular invasion; Prognosis","PeriodicalId":10021,"journal":{"name":"Chinese Journal of Hepatobiliary Surgery","volume":"26 1","pages":"38-43"},"PeriodicalIF":0.0,"publicationDate":"2020-01-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48618855","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-12-28DOI: 10.3760/CMA.J.ISSN.1007-8118.2019.12.009
Huaiteng Xiao, Deguo Zhang, Shunchang Zhou, Jingxiong Zhao, Guangjun Shi
Objective To investigate the expression and distribution of FNDC5/Irisin in pancreatic cancer tissues and adjacent tissues, and to analyze its correlation with clinicopathological features. Methods Collection of archived wax blocks from 64 patients diagnosed with pancreatic cancer after surgical treatment from January 2015 to December 2018 in the Department of Pathology, Affiliated Qingdao Municipal Hospital of Qingdao University, and 30 tissues collected intraoperatively from January 2016 to December 2018 Samples, all collected samples included tumor tissue and corresponding adjacent tissues (>2 cm from the tumor edge). Real-time quantitative PCR (qRT-PCR) and immunohistochemistry were used to detect the expression of FNDC5/Irisin mRNA and its positivity in pancreatic cancer tissues and adjacent tissues, and to analyze its relationship with clinicopathological features of pancreatic cancer. Results qRT-PCR showed that the expression of FNDC5/Irisin mRNA in pancreatic cancer tissues was higher than that in the corresponding adjacent pancreatic tissues, the difference was statistically significant (P 0.05), but FNDC5/Irisin expression was associated with liver and lymph node metastasis (P<0.05). Conclusion The positivity of FNDC5/Irisin in pancreatic cancer tissues is significantly higher than that in adjacent pancreatic tissues, and it is correlated with liver and lymph node metastasis in pancreatic cancer. Key words: Pancreatic neoplasms; FNDC5/Irisin; Immunohistochemistry
{"title":"Expression of FNDC5/Irisin in pancreatic cancer and its relationship with clinicopathological features","authors":"Huaiteng Xiao, Deguo Zhang, Shunchang Zhou, Jingxiong Zhao, Guangjun Shi","doi":"10.3760/CMA.J.ISSN.1007-8118.2019.12.009","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.1007-8118.2019.12.009","url":null,"abstract":"Objective \u0000To investigate the expression and distribution of FNDC5/Irisin in pancreatic cancer tissues and adjacent tissues, and to analyze its correlation with clinicopathological features. \u0000 \u0000 \u0000Methods \u0000Collection of archived wax blocks from 64 patients diagnosed with pancreatic cancer after surgical treatment from January 2015 to December 2018 in the Department of Pathology, Affiliated Qingdao Municipal Hospital of Qingdao University, and 30 tissues collected intraoperatively from January 2016 to December 2018 Samples, all collected samples included tumor tissue and corresponding adjacent tissues (>2 cm from the tumor edge). Real-time quantitative PCR (qRT-PCR) and immunohistochemistry were used to detect the expression of FNDC5/Irisin mRNA and its positivity in pancreatic cancer tissues and adjacent tissues, and to analyze its relationship with clinicopathological features of pancreatic cancer. \u0000 \u0000 \u0000Results \u0000qRT-PCR showed that the expression of FNDC5/Irisin mRNA in pancreatic cancer tissues was higher than that in the corresponding adjacent pancreatic tissues, the difference was statistically significant (P 0.05), but FNDC5/Irisin expression was associated with liver and lymph node metastasis (P<0.05). \u0000 \u0000 \u0000Conclusion \u0000The positivity of FNDC5/Irisin in pancreatic cancer tissues is significantly higher than that in adjacent pancreatic tissues, and it is correlated with liver and lymph node metastasis in pancreatic cancer. \u0000 \u0000 \u0000Key words: \u0000Pancreatic neoplasms; FNDC5/Irisin; Immunohistochemistry","PeriodicalId":10021,"journal":{"name":"Chinese Journal of Hepatobiliary Surgery","volume":"25 1","pages":"921-924"},"PeriodicalIF":0.0,"publicationDate":"2019-12-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42947162","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-12-28DOI: 10.3760/CMA.J.ISSN.1007-8118.2019.12.011
Ying Le, Ying-cun Li, Minman Zhang, H. Fang, Xiao-ke Dai
Objective To investigate the recovery of splenomegaly and hypersplenism after liver transplantation in children and explore the necessity of splenomegaly management before and during liver transplantation. Method The data of 22 children who were underwent liver transplantation with preoperative splenomegaly and hypersplenism who were not treated with splenomegaly before and during operation and with no recurrence of portal hypertension during postoperative follow-up in Department of Hepatobiliary Surgery, Children’s Hospital of Chongqing Medical University from December 2008 to January 2019 were collected. There were 13 male patients and 9 female patients with a median age of 6.5 months. The changes of erythrocyte, platelet, white blood cell and spleen length and thickness were analyzed by paired t-test before and at the 1st, 3rd and 6th months after surgery. Correlation analysis was performed on the change of spleen long diameter and thickness product and blood cell recovery. Results There were 21 cases of red blood cell decreased before surgery, with a mean value of(3.1±0.5)×1012/L, and(3.7±0.7)×1012/L, (4.6±0.6)×1012/L and (4.3±0.5)×1012/L at the 1st, 3rd and 6th months after operation, respectively. The differences between the preoperative and postoperative groups were statistically significant (P<0.05). There were 7 cases of thrombocytopenia before operation, with an average of (70.0±17.0)×109/L, and (191.0±129.0)×109/L, (156.0±79.0)×109/L and (167.0±63.0)×109/L at the 1st, 3rd and 6th months after operation, respectively. The differences between the first, third, and sixth months after surgery were significant compared with that before surgery (P<0.05). Leukocyte count decreased in 5 cases with an average value of (3.30±0.56)×109/L before surgery, (7.5±4.4)×109/L, (7.4±1.4)×109/L, (5.1± 2.5)×109/L at the 1st, 3rd and 6th months after operation, respectively. The differences between the two groups were significant (P<0.05). The average value of preoperative spleen length times thickness was (35.7±12.9) cm2 and at the 1st, 3rd, 6th month after surgery it was (26.2±8.1)cm2, (25.2±13.4)cm2, (27.2±7.4)cm2, respectively. The differences between the first, third months after surgery were significant compared with that before surgery (P<0.05). The white blood cell and platelet counts of children before and after surgery were correlated with spleen size negatively (correlation coefficients were -0.902, -0.933, respectively), and the differences were statistically significant (P<0.05). Conclusions In the early stage of liver transplantation, the size of spleen retracts to varying degrees, and hypersplenism can be alleviated continuously. Therefore, it is unnecessary to treat splenomegaly and hypersplenism before and during liver transplantation. Key words: Children; Liver transplantation; Hypersplenism
{"title":"The improvement of splenomegaly and hypersplenism after liver transplantation in children","authors":"Ying Le, Ying-cun Li, Minman Zhang, H. Fang, Xiao-ke Dai","doi":"10.3760/CMA.J.ISSN.1007-8118.2019.12.011","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.1007-8118.2019.12.011","url":null,"abstract":"Objective \u0000To investigate the recovery of splenomegaly and hypersplenism after liver transplantation in children and explore the necessity of splenomegaly management before and during liver transplantation. \u0000 \u0000 \u0000Method \u0000The data of 22 children who were underwent liver transplantation with preoperative splenomegaly and hypersplenism who were not treated with splenomegaly before and during operation and with no recurrence of portal hypertension during postoperative follow-up in Department of Hepatobiliary Surgery, Children’s Hospital of Chongqing Medical University from December 2008 to January 2019 were collected. There were 13 male patients and 9 female patients with a median age of 6.5 months. The changes of erythrocyte, platelet, white blood cell and spleen length and thickness were analyzed by paired t-test before and at the 1st, 3rd and 6th months after surgery. Correlation analysis was performed on the change of spleen long diameter and thickness product and blood cell recovery. \u0000 \u0000 \u0000Results \u0000There were 21 cases of red blood cell decreased before surgery, with a mean value of(3.1±0.5)×1012/L, and(3.7±0.7)×1012/L, (4.6±0.6)×1012/L and (4.3±0.5)×1012/L at the 1st, 3rd and 6th months after operation, respectively. The differences between the preoperative and postoperative groups were statistically significant (P<0.05). There were 7 cases of thrombocytopenia before operation, with an average of (70.0±17.0)×109/L, and (191.0±129.0)×109/L, (156.0±79.0)×109/L and (167.0±63.0)×109/L at the 1st, 3rd and 6th months after operation, respectively. The differences between the first, third, and sixth months after surgery were significant compared with that before surgery (P<0.05). Leukocyte count decreased in 5 cases with an average value of (3.30±0.56)×109/L before surgery, (7.5±4.4)×109/L, (7.4±1.4)×109/L, (5.1± 2.5)×109/L at the 1st, 3rd and 6th months after operation, respectively. The differences between the two groups were significant (P<0.05). The average value of preoperative spleen length times thickness was (35.7±12.9) cm2 and at the 1st, 3rd, 6th month after surgery it was (26.2±8.1)cm2, (25.2±13.4)cm2, (27.2±7.4)cm2, respectively. The differences between the first, third months after surgery were significant compared with that before surgery (P<0.05). The white blood cell and platelet counts of children before and after surgery were correlated with spleen size negatively (correlation coefficients were -0.902, -0.933, respectively), and the differences were statistically significant (P<0.05). \u0000 \u0000 \u0000Conclusions \u0000In the early stage of liver transplantation, the size of spleen retracts to varying degrees, and hypersplenism can be alleviated continuously. Therefore, it is unnecessary to treat splenomegaly and hypersplenism before and during liver transplantation. \u0000 \u0000 \u0000Key words: \u0000Children; Liver transplantation; Hypersplenism","PeriodicalId":10021,"journal":{"name":"Chinese Journal of Hepatobiliary Surgery","volume":"25 1","pages":"930-933"},"PeriodicalIF":0.0,"publicationDate":"2019-12-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45467645","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-12-28DOI: 10.3760/CMA.J.ISSN.1007-8118.2019.12.013
Jin Huang, Shenzong Rao, Ji-jun Hu, Changgang Xiang, Min Zhang, Xueliang Lu, Haoran Sun, Jian Li
Objective Analysis of the effect of triggering receptor-1 expressed on myeloid cells (TREM-1) in nonalcoholic fatty liver disease (NAFLD) and the mechanism. Methods The oleic acid-treated HepG2 cells were divided into model group, overexpression group, interference group A, interference group B and negative control group. The mouse model of NAFLD was generated and randomly divided into (nuclear factor-κB) NF-κB inhibition group, protein kinase B (AKT) inhibition group, knockout group A, knockout group B and control group. The expression of inflammatory factors and TREM-1 in liver tissue was detected by PCR, and fat accumulation was detected by oil red O staining. Western blotting was used to detect the expression of TREM-1 and signaling pathway proteins, and HE staining was used to detect liver tissue changes. Results TREM-1 was up-regulated in liver tissue of NAFLD mice [(0.936±0.127) vs. (0.432±0.105)] and in oleic acid-treated HepG2 cells. In oleic acid-treated HepG2 cells, overexpression of TREM-1 increased inflammatory factor expression and increased lipid droplets; inhibition of TREM-1 expression decreased inflammatory factor expression, and lipid droplets decreased. Knockout of TREM-1 and inhibition of NF-κB in NAFLD mice reduced hepatocyte inflammatory factor expression and reduced liver damage; knockout of TREM-1 and inhibition of AKT reduced liver tissue lipids and drops accumulate. Conclusions The overexpression of TREM-1 in NAFLD mice liver tissue can regulate inflammatory factor expression and lipid droplets through NF-κB and AKT signal pathway. TREM-1 might be a potential therapeutic target of NAFLD. Key words: Inflammation; Nonalcoholic fatty liver disease; Triggering receptor-1 expressed on myeloid cells; Lipid accumulation
{"title":"Effects and mechanism of TREM-1 on inflammatory response and lipid metabolism in mice with nonalcoholic fatty liver disease","authors":"Jin Huang, Shenzong Rao, Ji-jun Hu, Changgang Xiang, Min Zhang, Xueliang Lu, Haoran Sun, Jian Li","doi":"10.3760/CMA.J.ISSN.1007-8118.2019.12.013","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.1007-8118.2019.12.013","url":null,"abstract":"Objective \u0000Analysis of the effect of triggering receptor-1 expressed on myeloid cells (TREM-1) in nonalcoholic fatty liver disease (NAFLD) and the mechanism. \u0000 \u0000 \u0000Methods \u0000The oleic acid-treated HepG2 cells were divided into model group, overexpression group, interference group A, interference group B and negative control group. The mouse model of NAFLD was generated and randomly divided into (nuclear factor-κB) NF-κB inhibition group, protein kinase B (AKT) inhibition group, knockout group A, knockout group B and control group. The expression of inflammatory factors and TREM-1 in liver tissue was detected by PCR, and fat accumulation was detected by oil red O staining. Western blotting was used to detect the expression of TREM-1 and signaling pathway proteins, and HE staining was used to detect liver tissue changes. \u0000 \u0000 \u0000Results \u0000TREM-1 was up-regulated in liver tissue of NAFLD mice [(0.936±0.127) vs. (0.432±0.105)] and in oleic acid-treated HepG2 cells. In oleic acid-treated HepG2 cells, overexpression of TREM-1 increased inflammatory factor expression and increased lipid droplets; inhibition of TREM-1 expression decreased inflammatory factor expression, and lipid droplets decreased. Knockout of TREM-1 and inhibition of NF-κB in NAFLD mice reduced hepatocyte inflammatory factor expression and reduced liver damage; knockout of TREM-1 and inhibition of AKT reduced liver tissue lipids and drops accumulate. \u0000 \u0000 \u0000Conclusions \u0000The overexpression of TREM-1 in NAFLD mice liver tissue can regulate inflammatory factor expression and lipid droplets through NF-κB and AKT signal pathway. TREM-1 might be a potential therapeutic target of NAFLD. \u0000 \u0000 \u0000Key words: \u0000Inflammation; Nonalcoholic fatty liver disease; Triggering receptor-1 expressed on myeloid cells; Lipid accumulation","PeriodicalId":10021,"journal":{"name":"Chinese Journal of Hepatobiliary Surgery","volume":"25 1","pages":"937-941"},"PeriodicalIF":0.0,"publicationDate":"2019-12-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42492119","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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