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Effects of A- and B-type starch granules on composition, structural, thermal, morphological, and pasting properties of starches from diverse wheat varieties A 型和 B 型淀粉颗粒对不同小麦品种淀粉的成分、结构、热力、形态和糊化性能的影响
Pub Date : 2023-12-04 DOI: 10.1002/fbe2.12068
Rajesh Kumar, Narpinder Singh, Bhupendar Singh Khatkar

The distribution of A- and B-type-sized starch granules plays a deciding role in controlling the physicochemical, structural, morphological, and functional attributes of wheat starch. Starches of three Indian wheat varieties, viz. DBW 16, WH 147, and WH 542, were fractionated into A- and B-type starch granules and further evaluated for their influence on various attributes of wheat starches using different analytical tools like X-ray diffraction, scanning electron microscopy (SEM), differential scanning calorimetry, and rapid viscoanalyzer. SEM revealed that the size of large granules (A-type) ranged from 12.6 to 36.4 µm and small granules (B-type) varied from 2.53 to 7.52 µm. The amylose content was significantly higher for A-type starch ranging from 26.6% to 29.68% than B-type starch ranging from 19.20% to 22.38%. The highest swelling power was observed for B-type granules, followed by native and A-type granules, and similar trend was noticed for water absorption. Pasting viscosities of A-type granules were higher than B-type for starches of all wheat varieties. A higher pasting temperature was observed in B-type starch granules, suggesting more resistance to swell during the heating process. X-ray diffraction of wheat starches showed A-type pattern of crystallinity, variety DBW 16 (27%) showed the highest relative crystallinity and intensities of peaks in comparison to varieties WH 147 (23.5%) and WH 542 (22.4% as observed in diffractograms and well supported by Fourier transforms infrared spectroscopy. Fractionated large granules of wheat starches exhibited a higher gelatinization temperature than smaller granules and native starches for all the varieties. It was also observed that A-type granules had higher onset temperature comparatively, which suggested that high energy is required in gelatinization due to more ordered arrangement of starch molecules.

A型和B型淀粉颗粒的分布对小麦淀粉的理化、结构、形态和功能属性起着决定性的控制作用。采用不同的分析工具,如X射线衍射、扫描电镜、差示扫描量热法和快速粘度分析仪,对3个印度小麦品种dbw16、WH 147和WH 542的淀粉进行了分馏,并进一步评估了它们对小麦淀粉各种属性的影响。扫描电镜显示,大颗粒(A型)的尺寸在12.6 ~ 36.4µm之间,小颗粒(B型)的尺寸在2.53 ~ 7.52µm之间。A型淀粉直链淀粉含量为26.6% ~ 29.68%,显著高于B型淀粉的19.20% ~ 22.38%。B型颗粒的溶胀力最高,其次是天然颗粒和A型颗粒,吸水率也有类似的趋势。所有小麦品种淀粉的A型颗粒黏度均高于B型。在B型淀粉颗粒中观察到较高的糊化温度,表明在加热过程中更耐膨胀。小麦淀粉的X射线衍射表现为A型结晶度,品种dbw16(27%)的相对结晶度和峰强度均高于品种WH 147(23.5%)和WH 542(22.4%),傅里叶变换红外光谱也证实了这一点。各品种分馏大颗粒小麦淀粉的糊化温度均高于小颗粒小麦淀粉和天然小麦淀粉。A型颗粒具有较高的起始温度,这表明由于淀粉分子排列更有序,在糊化过程中需要较高的能量。
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引用次数: 0
A review on bioactive compounds and health benefits of Baccaurea ramiflora 关于苧麻的生物活性化合物和健康益处的综述
Pub Date : 2023-12-03 DOI: 10.1002/fbe2.12069
Shubham Rohilla

The purpose of this review is to explore the underutilized and underexploited Burmese grape (Baccaurea ramiflora) as a valuable plant food crop grown in the northeastern region of India. Despite its seasonal availability, this fruit is used in various culinary applications and local delicacies. It is noteworthy for its abundant natural bioactive compounds that offer diverse health benefits. These bioactive compounds have demonstrated properties such as anti-inflammatory, antiobesity, cytotoxic, and antidiabetic effects. This review compiles and analyzes the most recent research findings concerning the bioactive compounds found in Burmese grape's leaves, fruit, and seeds. Additionally, it investigates the physicochemical properties and nutritional composition of the fruit pulp and seed oil. The synthesis of this information provides a comprehensive understanding of the plant's phytochemical content, including essential components like vitamin C, iron, potassium, alkaloids, saponins, and phenolic acids, which contribute to its medicinal and health-promoting attributes. The results presented in this review shed light on the potential applications of the Burmese grape and its bioactive compounds in various industrial sectors. With the growing interest in functional and nutraceutical products, the insights gathered here offer promising opportunities for leveraging the nutritional and health-enhancing benefits of this fruit in the food and pharmaceutical industries.

本综述的目的是探讨缅甸葡萄(Baccaurea ramiflora)作为一种有价值的粮食作物在印度东北部地区的未充分利用和开发。尽管它的季节性供应,这种水果被用于各种烹饪应用和当地美食。值得注意的是其丰富的天然生物活性化合物,提供多种健康益处。这些生物活性化合物具有抗炎、抗肥胖、细胞毒性和抗糖尿病作用等特性。本文对缅甸葡萄叶、果实和种子中生物活性成分的最新研究成果进行了综述和分析。此外,还对其果肉和籽油的理化性质和营养成分进行了研究。这些信息的综合提供了对植物化学成分的全面了解,包括维生素C、铁、钾、生物碱、皂苷和酚酸等必需成分,这些都有助于其药用和促进健康的属性。本文综述了缅甸葡萄及其生物活性化合物在各个工业领域的潜在应用前景。随着人们对功能性和营养保健品的兴趣日益浓厚,这里收集的见解为在食品和制药行业利用这种水果的营养和健康益处提供了有希望的机会。
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引用次数: 0
Edible gums—An extensive review on its diverse applications in various food sectors
Pub Date : 2023-11-20 DOI: 10.1002/fbe2.12067
Vijayasri Kadirvel, Gururaj P. Narayana

In recent years, the extraction, processing, and application of edible gums, in the food and healthcare sector has skyrocketed and extensively explored. Edible gums are derived from multiple sources such as plants/trees, animals, microorganisms, and seaweed. Edible gums are typically employed to perform certain functions including modification of rheology, gel formation, stability, encapsulation, and fat replacement. The unique interaction of edible gums with food molecules enables the achievement of desired characteristics of specific food products belonging to various sectors. This review delivers a comprehensive understanding of the classification and characterization of edible gums and their applications in the food industry. Despite several advantages offered by edible gums, there exist certain challenges that are encountered during commercialization by manufacturers and proposed strategies to overcome the hurdles, regulatory aspects, and safety of edible gums upon consumption have been highlighted in this review.

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引用次数: 0
Engineering a sustainable protein revolution: Recent advances in cultured meat production 设计可持续的蛋白质革命:人造肉生产的最新进展
Pub Date : 2023-10-08 DOI: 10.1002/fbe2.12066
Niladri Krishna Roy, Sandeep Panda, Gargi Dey

Emerging as a food of the future, cultured meat is created by growing animal cells outside the organism instead of relying on traditional animal rearing and slaughtering practices. This innovative approach shows great promise in addressing challenges associated with resource utilization, environmental pollution, and public health concerns often encountered in conventional livestock production. In the past decade, popularity of cultured meat has grown enough to be considered as a novel food in regions like Europe, the United Kingdom, and China. This paper discusses the recent advancements in technologies for sustainable cultured meat production. It examines the latest developments in cell sources, cell culture media optimization, bioreactor design, 3D printing, and tissue engineering approaches, which have greatly enhanced the efficiency and scalability of cultured meat production. This food can have a tremendous application as a novel functional food. This review explores the future possibility of applying cultured meat matrix for delivery of hydrophobic nutraceuticals, prebiotics, and probiotics (Bacillus sp).

人造肉作为一种未来的食品,是通过在生物体外培养动物细胞来创造的,而不是依赖于传统的动物饲养和屠宰方法。这一创新方法在解决与传统畜牧业生产中经常遇到的资源利用、环境污染和公共卫生问题相关的挑战方面显示出巨大的希望。在过去的十年里,人造肉在欧洲、英国和中国等地区的受欢迎程度已经上升到足以被视为一种新食物的程度。本文讨论了可持续养殖肉类生产技术的最新进展。它考察了细胞来源、细胞培养基优化、生物反应器设计、3D打印和组织工程方法的最新发展,这些方法大大提高了培养肉生产的效率和可扩展性。作为一种新型的功能性食品,该食品具有广阔的应用前景。本文综述了利用培养肉基质输送疏水营养品、益生元和益生菌(芽孢杆菌)的未来可能性。
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引用次数: 0
Improved colloidal and oxidative stability of krill oil using natural biopolymers via spray drying microencapsulation 喷雾干燥微胶囊化利用天然生物聚合物改善磷虾油的胶体和氧化稳定性
Pub Date : 2023-09-29 DOI: 10.1002/fbe2.12064
Xing Li, Jiande Wu, Meiyu Liu

Krill oil possesses strong antioxidant activity but has limited applications in the food industry due to its poor aqueous solubility, strong off-flavours and susceptibility to oxidative degradation. This study was aimed to improve the colloidal and oxidative stability of krill oil via microencapsulation using spray drying. Whey protein isolate (WPI), maltodextrin (MD), carboxymethyl starch (CMS) and n-octenylsuccinate starch (OSAS) were used as the microcapsule wall materials. The results showed that WPI+MD microcapsules had the optimum characterised properties compared to WPI+CMS and WPI+OSAS microcapsules. WPI+MD microcapsules possessed the highest encapsulation efficiency of 76.59%. The optimal air inlet temperature was 165°C, providing a high krill oil encapsulation efficiency and reducing concaveness on the microcapsule surfaces. Krill oil was distributed homogeneously inside the capsules and presented a good colloidal stability during an extended storage time. The antioxidant capacity against heat was simultaneously increased owing to the improved oxidative stability by microencapsulation. Consequently, this study provides useful guidelines for improving the stability and application of krill oil in the food industry.

磷虾油具有很强的抗氧化活性,但由于其水溶性差、强烈的异味和易氧化降解,在食品工业中的应用有限。本研究旨在通过喷雾干燥微胶囊化来提高磷虾油的胶体稳定性和氧化稳定性。以乳清蛋白分离物(WPI)、麦芽糊精(MD)、羧甲基淀粉(CMS)和正辛基琥珀酸淀粉(OSAS)为微胶囊壁材。结果表明,与WPI+CMS和WPI+OSAS微胶囊相比,WPI+MD微胶囊具有最佳的表征性能。WPI+MD微胶囊的包封效率最高,为76.59%。最佳进气温度为165°C,提供了高的磷虾油包封效率,并降低了微胶囊表面的凹陷度。磷虾油在胶囊内均匀分布,在延长的储存时间内表现出良好的胶体稳定性。由于微胶囊化提高了氧化稳定性,同时提高了抗氧化能力。因此,本研究为提高磷虾油的稳定性和在食品工业中的应用提供了有用的指导。
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引用次数: 0
Marang fruit (Artocarpus Odoratissimus) waste: A promising resource for food and diverse applications: A review of its current status, research opportunities, and future prospects 马郎果(Artocarpus Odoratissimus)废弃物:一种具有广阔应用前景的食品资源:综述其现状、研究机会及未来展望
Pub Date : 2023-09-26 DOI: 10.1002/fbe2.12065
Marjun C. Alvarado

Marang fruit is commonly found and available in various Southeast Asian regions, notably in countries such as Malaysia, the Philippines, and Indonesia. Marang fruit waste, despite its promising potential, remains underutilized in various applications. This review article explores the potential of Marang fruit waste as a valuable resource for various applications, with a focus on its utilization in the food industry, including seed flour, starch, snacks, and essential bioactive compounds. Beyond the food sector, the article also investigates its applications as biosorbents/adsorbents and an energy source. The study identifies promising research opportunities such as investigating its potential as a fuel source, as well as for the production of cellulose and nanocellulose which is now gaining relevant attention in various industries. However, the seasonal availability of Marang fruit and its limited distribution in specific Southeast Asian regions present challenges. This article aims to provide a comprehensive understanding of the current status, highlight the areas that require further investigation, and future prospects of Marang fruit waste, highlighting its significance as a sustainable resource with multifaceted applications.

摘要:在东南亚地区,特别是在马来西亚、菲律宾和印度尼西亚等国家,普遍发现并可获得玛朗果。马郎果渣虽然潜力巨大,但在各种应用中仍未得到充分利用。本文综述了马郎果渣作为一种有价值资源的应用潜力,重点介绍了其在食品工业中的应用,包括种子面粉、淀粉、零食和必需生物活性化合物。在食品领域之外,文章还研究了其作为生物吸附剂/吸附剂和能源的应用。该研究确定了有前途的研究机会,例如调查其作为燃料来源的潜力,以及纤维素和纳米纤维素的生产,这在各个行业都得到了相关的关注。然而,马郎水果的季节性供应及其在特定东南亚地区的有限分布带来了挑战。本文旨在全面介绍马郎果废弃物的研究现状、研究领域以及未来发展前景,强调其作为可持续资源的重要性。
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引用次数: 0
Quorum-sensing in Acetobacter pasteurianus and its effect on acetic acid fermentation 巴氏醋杆菌群体感应及其对醋酸发酵的影响
Pub Date : 2023-09-26 DOI: 10.1002/fbe2.12063
Hang Zhao, Zhiwei Feng, Xueli Han, Jiao Wang, Ying Xu, Weizhao Li, Yu Zheng, Min Wang

Quorum-sensing (QS) is an essential mechanism of microbial self-regulation. In this work, the presence of QS in Acetobacter pasteurianus was demonstrated, and N-acyl-homoserine lactones N-C4-HSL and N-C8-HSL were identified as the signal molecules. The exogenous addition of N-C4-HSL, N-C8-HSL, N-C10-HSL, and N-C12-HSL result in the reduction in biomass, and lead to 11.56%, 11.91%, 7.94%, and 4.69% decrease in acetic acid production, respectively, when compared with the control. A hypothetical N-acylhomoserine lactonase (AHL lactonase) 0046 was overexpressed in A. pasteurianus CGMCC 3089, and the recombinant strain exhibites a 17.38% and 12.75% increase in the biomass and the acetic acid production rate, respectively. Those results prove the negative regulation of QS on acetic acid fermentation, and overexpression of 0046 protein alleviates this negative effect.

群体感应(QS)是微生物自我调节的重要机制。在这项工作中,证明了QS在巴氏醋杆菌中的存在,并将N-酰基-丝氨酸内酯N-C4-HSL和N-C8-HSL鉴定为信号分子。外源添加N-C4-HSL、N-C8-HSL、N-C10-HSL和N-C12-HSL导致生物量减少,与对照相比,乙酸产量分别减少11.56%、11.91%、7.94%和4.69%。假定的N-酰基高丝氨酸内酯酶(AHL内酯酶)0046在A.pasteurianus CGMCC 3089中过表达,重组菌株的生物量和乙酸生产速率分别增加17.38%和12.75%。这些结果证明了QS对乙酸发酵的负调控,0046蛋白的过表达减轻了这种负调控。
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引用次数: 0
Engineering the oleaginous yeast Yarrowia lipolytica to produce nutraceuticals: From metabolic design to industrial applications 工程含油酵母解脂Yarrowia生产营养品:从代谢设计到工业应用
Pub Date : 2023-09-26 DOI: 10.1002/fbe2.12062
Yang Gu, Xueyao Lu, Tongkang Liu, Yanjin Song, Ellie Sang, Shuran Ding, Long Liu, Feng Xue, Peng Xu

The oleaginous yeast, Yarrowia lipolytica, has garnered much attention and is gaining recognition as a potential industrial platform for nutraceutical production. The superior properties of Y. lipolytica include its transparent inherited background, broad substrate spectrum, and superior environmental tolerance. Specifically, advances in genetic tools and engineering strategies have further widened its applications in metabolic engineering and biomanufacturing. In this review, we summarized developments in synthetic biology and engineering strategies for constructing Y. lipolytica to produce nutraceuticals. We also proposed perspectives on the limitations and challenges in developing microbial Y. lipolytica factories.

含油酵母Yarrowia lipolytica已经引起了人们的广泛关注,并被公认为一个潜在的营养品生产工业平台。溶脂乳杆菌的优良特性包括其透明的遗传背景、宽的底物光谱和优异的环境耐受性。具体而言,基因工具和工程策略的进步进一步扩大了其在代谢工程和生物制造中的应用。在这篇综述中,我们总结了合成生物学的发展和构建溶脂酵母生产营养品的工程策略。我们还对开发微生物溶脂酵母工厂的局限性和挑战提出了看法。
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引用次数: 0
Antioxidant capacity of wheat bran fermented with gut indigenous Bifidobacterium and its antagonistic effect on food-related pathogens in vitro 肠道固有双歧杆菌发酵麦麸的抗氧化能力及其对食品相关病原体的拮抗作用
Pub Date : 2023-08-16 DOI: 10.1002/fbe2.12061
Natsumi Takei, Ayumi Miyashita, Takashi Kuda, Yumeng Xia, Kazuya Koga, Ayaka Nakamura, Hajime Takahashi

Wheat bran (WB) has several health-promoting effects. This study aimed to identify gut bacteria that increase after WB consumption and assess their functionality. Human stool samples obtained from healthy volunteers were inoculated into culture broth with or without 2% (w/v) WB and incubated under anaerobic conditions for 24 h. The microbiota in the cultures was analysed using 16S rRNA (V4) gene amplicon sequencing. The addition of WB decreased the pH from 6.9 to 5.9 (p < 0.05) and increased the acetate level by 1.6 times. Although the microbiota differed across individuals, butyrate-producing genera (Faecalibacterium and Roseburia), Blautia, and Bifidobacterium spp. were abundant in cultures supplemented with WB. Bifidobacterium pseudocatenulatum and B. adolescentis, isolated as WB-responsible gut indigenous bacteria (WB-RIBs), were found to ferment WB. The WB-RIBs increased the 1,1-diphenyl-picrylhydrazyl and superoxide anion radical-scavenging capacities of WB-supplemented cultures. Further, these WB-RIBs suppressed the growth of Salmonella Typhimurium, Staphylococcus aureus, and Bacillus cereus in WB-supplemented brain heart infusion broth. These results suggest that compounds present in WB, along with WB-RIBs, affect the gut environment. Further studies should be conducted to elucidate the mechanisms underlying the interactions between WB and WB-RIBs.

麦麸(WB)具有多种健康促进作用。这项研究旨在识别WB摄入后增加的肠道细菌,并评估其功能。将从健康志愿者获得的人类粪便样品接种到含有或不含有2%(w/v)WB的培养液中,并在厌氧条件下孵育24小时 h.使用16S rRNA(V4)基因扩增子测序分析培养物中的微生物群。WB的加入使pH从6.9降低到5.9(p <; 0.05),并使乙酸盐水平增加1.6倍。尽管微生物群在个体之间存在差异,但丁酸盐产生属(Faecalibacterium和Roseburia)、Blautia和双歧杆菌属在补充WB的培养物中含量丰富。假专利双歧杆菌(Bifidobacterium pseudoatenulatum)和青春双歧杆菌(B.adoriscentis)被分离为负责WB的肠道固有细菌(WB-RIBs),它们能够发酵WB。WB RIBs增加了补充WB的培养物的1,1-二苯基-丙烯酰肼和超氧阴离子自由基清除能力。此外,在补充WB的脑心灌注肉汤中,这些WB-RIB抑制了鼠伤寒沙门氏菌、金黄色葡萄球菌和蜡样芽孢杆菌的生长。这些结果表明,WB中存在的化合物以及WB-RIB会影响肠道环境。应进行进一步的研究,以阐明WB和WB-RIB之间相互作用的机制。
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引用次数: 0
Effect of composition on the structure and digestibility of starch in Idli batter 成分对伊德利面糊淀粉结构和消化率的影响
Pub Date : 2023-08-09 DOI: 10.1002/fbe2.12059
Leeann Roy, Somanathan Karthiga Reshmi, Jeyan Arthur Moses, Chinnaswamy Anandharamakrishnan

This study investigates the effects of varying the proportion of parboiled rice, raw rice, mixed rice, and black gram on idli batter structure and starch digestibility. A total of six different batters (batters 1 to 6) were prepared by varying the composition of raw material. Batter 1 (Parboiled Rice: Black gram dal: Baking soda), Batter 2 (Parboiled Rice: Black gram dal: Yeast), Batter 3 (Parboiled Rice: Raw Rice: Mixed Rice: Black gram dal), Batter 4 (Raw Rice: Mixed Rice: Black gram dal), Batter 5 (Raw rice: Yeast) and Batter 6 (Parboiled Rice: Black gram dal). In comparison to the other five batters, batter 6 (parboiled rice (3): Black gram dal (1)) exhibited superior texture, sensory, enzyme activity, and better digestibility. Structure changes in starch for batter 6 were noticeable until day five and complete degradation was observed after day seven, whereas in other batters, the structure degraded from 3rd day of storage. Batter 6 exhibited the highest protein content (0.6 mg/g to 0.3 mg/g) and lowest reducing sugar content (4.51 mg/g to 6.66 mg/g) from day 1 to day 7 of the storage period. The difference in amylose content from day 1 to day 7 (27.80% to 26.75%) was low compared to others. The starch digestibility index of batter 6 was 40.35% on day 1 and 68.96% on day 7 which was comparatively less compared to other prepared batters. As a result, the proportion of dal and parboiled rice used in batter preparation significantly influences its storage, digestibility, and enzyme activity. Thus, studying the method and proportion of idli preparation helps us understand how the batter is stored, digested, and modified, thereby improving our understanding of Indian cuisine.

本研究探讨了不同比例的半熟米、生米、混合米和黑克对idli面糊结构和淀粉消化率的影响。通过改变原料的组成来制备总共六种不同的电池(电池1至6)。面糊1(糙米:黑克豆:小苏打)、面糊2(糙米:黑克豆:酵母)、面饼3(糙米:生米:混合米:黑克糖)、面皮4(生米:混米:黑克糖),面糊5(生米:酵母)和面糊6(糙米:黑色克糖)。与其他五种面糊相比,面糊6(半熟米(3):黑木豆(1))表现出优异的质地、感官、酶活性和更好的消化率。面糊6的淀粉结构变化在第五天之前是明显的,第七天之后观察到完全降解,而在其他面糊中,结构从储存的第三天开始降解。Batter 6表现出最高的蛋白质含量(0.6 mg/g至0.3 mg/g)和最低还原糖含量(4.51 mg/g至6.66 mg/g)。从第1天到第7天直链淀粉含量的差异(27.80%到26.75%)与其他相比是低的。面糊6的淀粉消化率指数在第1天为40.35%,在第7天为68.96%,与其他制备的面糊相比相对较低。因此,在面糊制备中使用的木豆和半熟米的比例显著影响其储存、消化率和酶活性。因此,研究idli的制备方法和比例有助于我们了解面糊是如何储存、消化和改性的,从而提高我们对印度美食的理解。
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引用次数: 0
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Food Bioengineering
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