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Research progress of probiotics and fermented feed effects on pork quality 益生菌和发酵饲料对猪肉质量影响的研究进展
Pub Date : 2024-03-23 DOI: 10.1002/fbe2.12082
Xiao Lian, Mingyu Shi, Qinlu Lin, Ying Liang, Lingyu Zhang

With the growth of the global population and the development of the economy, the demand for delicious and nutritious meat is increasing. However, the pursuit of high productivity and a high lean meat rate in pig breeding has led to a serious decline in meat quality. How to moderately improve the quality and flavor of pork through nutritional supplementation is of great significance in meeting consumers' demand for high-quality and delicious pork. Recent studies have shown that fermented feed using probiotics can not only improve the nutritional quality and utilization rate of agricultural by-products, but also play an important role in improving meat color, increasing intramuscular fat content, regulating the flavor of pork, leading to a sustainable development of agriculture and husbandry. In this paper, we will review the current situation of the probiotics used in fermented feed, the effects of fermentation on the improvement of feed quality, the influence and mechanism of fermented feed on the growth performance, meat quality, and flavor of pigs, and so forth, aiming to provide theoretical basis and technical support for the research and development of fermented feed and its application in the production of high-quality pork.

随着全球人口的增长和经济的发展,人们对肉质鲜美、营养丰富的肉类需求与日俱增。然而,养猪业一味追求高生产率和高瘦肉率,导致肉质严重下降。如何通过营养补充适度提高猪肉的品质和风味,对于满足消费者对优质美味猪肉的需求具有重要意义。近年来的研究表明,利用益生菌发酵饲料不仅能提高农副产品的营养品质和利用率,还能在改善肉色、增加肌内脂肪含量、调节猪肉风味、带动农牧业可持续发展等方面发挥重要作用。本文将综述发酵饲料中益生菌的应用现状,发酵对饲料品质改善的作用,发酵饲料对猪生长性能、肉质、风味的影响及作用机理等,旨在为发酵饲料的研发及其在优质猪肉生产中的应用提供理论依据和技术支持。
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引用次数: 0
Industry 4.0 technologies for cultivated meat manufacturing 用于肉类加工的工业 4.0 技术
Pub Date : 2024-03-13 DOI: 10.1002/fbe2.12080
Sishir K. Kamalapuram, Deepak Choudhury

Industry 4.0 integrates the physical, digital, and biological realms by applying digital automation in systems, processes, and manufacturing facilities. Industry 4.0 is actively shaping the development of intelligent food processing industries in cultivated meat (CM) sector. This integration plays a crucial role in accelerating progress within the global CM sector, facilitating the achievement of its objectives related to food sustainability, security, human health, environmental concerns, and hygiene. Incorporating Industry 4.0 into CM manufacturing systems empowers upstream and downstream production processes to become more intelligent and capable of self-optimisation. However, enabling rapid adoption of Industry 4.0 by emerging startups and small to medium-sized enterprises in the CM industry necessitates a thorough understanding of prerequisites and evaluation of technological and biological limitations. Challenges include the substantial initial costs associated with establishing Industry 4.0 infrastructure, robust cybersecurity measures to ensure effective risk management, and acquiring skilled professionals proficient in both operational and maintenance roles. Integrating Industry 4.0 with the evolving CM sector presents an exciting opportunity to foster business-to-business investments across various domains, including local markets, export opportunities, and the broader global consumer ecosystem.

工业 4.0 通过在系统、流程和生产设施中应用数字自动化,整合了物理、数字和生物领域。工业 4.0 正在积极推动肉类加工业的智能化发展。这种整合在加快全球肉类加工行业的发展方面发挥着至关重要的作用,有助于实现与食品可持续性、安全、人类健康、环境问题和卫生相关的目标。将工业 4.0 融入中药制造系统可使上游和下游生产流程变得更加智能,并能够自我优化。然而,要让中药行业的新兴初创企业和中小型企业快速采用工业 4.0,就必须充分了解先决条件,并评估技术和生物方面的限制。所面临的挑战包括建立工业 4.0 基础设施所需的巨额初始成本、确保有效风险管理的强大网络安全措施,以及获得精通操作和维护的熟练专业人员。将工业 4.0 与不断发展的中药行业相结合,为促进各领域的企业对企业投资提供了一个令人兴奋的机会,包括本地市场、出口机会和更广泛的全球消费生态系统。
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引用次数: 0
Potential of utilizing almond hull extract for filamentous fungi production by submerged cultivation 利用杏仁壳提取物通过浸没式栽培生产丝状真菌的潜力
Pub Date : 2024-03-08 DOI: 10.1002/fbe2.12079
Lin Cao, Tyler J. Barzee, Hamed M. El Mashad, Zhongli Pan, Ruihong Zhang

Almond hulls can be used as an inexpensive source of nutrients for producing fungi that can be used as nontraditional foods such as alternative protein, probiotics, and food ingredients. Nutrients were extracted from almond hulls using hot water and their utility in cultivating filamentous fungus Aspergillus awamori (A. awamori) in submerged cultivation was investigated using batch fermentation in flasks with passive aeration. The almond hulls extract supplied all the nutrients required for A. awamori growth. The uptake preference of various sugars was investigated and the growth kinetic parameters of A. awamori were determined. Utilization of sugars by A. awamori grown in almond hull extract proceeded sequentially with preference for glucose followed by sucrose, fructose, and then xylose. Compared to potato dextrose broth medium, the fungal biomass produced using almond hull extract as growth medium had higher protein (19.59%) and lower fat (1.50%) contents. The modified Gompertz model described the kinetics of A. awamori well with a lag phase of 0.18 days and a specific growth rate of 1.77 d−1.

杏仁壳可作为生产真菌的廉价营养源,而真菌可用作替代蛋白质、益生菌和食品配料等非传统食品。研究人员用热水从杏仁壳中提取了营养物质,并在被动通气的烧瓶中采用批量发酵法研究了其在浸没式培养丝状真菌 awamori 曲霉(A. awamori)中的效用。杏仁壳提取物提供了 awamori 曲霉生长所需的全部养分。研究了 awamori 对各种糖类的吸收偏好,并确定了其生长动力学参数。在杏仁壳提取物中生长的 A. awamori 对糖类的利用依次为葡萄糖、蔗糖、果糖和木糖。与马铃薯葡萄糖肉汤培养基相比,使用杏仁壳提取物作为生长培养基产生的真菌生物量蛋白质含量更高(19.59%),脂肪含量更低(1.50%)。改良的贡珀兹模型很好地描述了 A. awamori 的生长动力学,其滞后期为 0.18 天,特定生长率为 1.77 d-1。
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引用次数: 0
Dried algae as potential functional ingredient in fresh cheese 干海藻作为新鲜奶酪中潜在的功能性配料
Pub Date : 2024-03-08 DOI: 10.1002/fbe2.12077
Daniela M. Borges, Susana C. Ribeiro, Sofia P. M. Silva, Célia C. G. Silva

Algae have attracted the attention of researchers and consumers due to their health-promoting bioactive compounds. In addition, the high content of polysaccharides may confer important technological properties when included in foods with high moisture content, such as fresh cheese. In this study, two dried algae species—Fucus spiralis and Petalonia binghamiae, were added to fresh cheese to improve its functional properties. Syneresis was evaluated by water holding capacity (WHC) and measurement of weight loss of the cheeses during storage at 4°C. Cheeses were also analysed for antioxidant activity, composition, and organoleptic properties. The addition of dried seaweed significantly reduced the weight loss of fresh cheese during storage (p < 0.05), which is normally associated with a loss of sensory properties. A significant increase (p < 0.05) in antioxidant activity was also observed in the cheeses with the addition of both algae. Except for the cheese with the addition of F. spiralis (1 g/100 g), the chemical composition did not change (p > 0.05) when the algae were added to the cheeses. Cheese with the addition of dried F. spiralis showed the best results in the sensory tests, consumer preference, and purchase intention.

藻类因其具有促进健康的生物活性化合物而备受研究人员和消费者的关注。此外,在新鲜奶酪等水分含量较高的食品中加入高含量的多糖,可赋予其重要的技术特性。本研究在新鲜奶酪中添加了两种干海藻--螺旋藻和瓣藻,以改善其功能特性。通过持水量(WHC)和测量奶酪在 4°C 储存期间的重量损失来评估滞后性。此外,还对奶酪的抗氧化活性、成分和感官特性进行了分析。在奶酪中添加干海藻后,新鲜奶酪在储存期间的重量损失明显减少(p 0.05)。添加了干螺旋藻的奶酪在感官测试、消费者偏好和购买意向方面都显示出最佳效果。
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引用次数: 0
Probiotics: Health benefits, food application, and colonization in the human gastrointestinal tract 益生菌:益生菌:对健康的益处、在食品中的应用以及在人体胃肠道中的定植
Pub Date : 2024-03-08 DOI: 10.1002/fbe2.12078
Li Ying Jessie Lau, Siew Young Quek

Probiotics have become increasingly popular over the past two decades due to the continuously expanding scientific evidence indicating their beneficial effects on human health. Therefore, they have been applied in the food industry to produce functional food, which plays a significant role in human health and reduces disease risk. However, maintaining the viability of probiotics and targeting the successful delivery to the gastrointestinal tract remain two challenging tasks in food applications. Specifically, this paper reviews the potentially beneficial properties of probiotics, highlighting the use and challenges of probiotics in food application and the associated health benefits. Of foremost importance, this paper also explores the potential underlying molecular mechanisms of the enhanced effect of probiotics on gastrointestinal epithelial cells, including a discussion on various surface adhesion-related proteins on the probiotic cell surface that facilitate colonization.

在过去二十年里,益生菌越来越受到人们的青睐,因为越来越多的科学证据表明,益生菌对人类健康有益。因此,益生菌已被应用于食品工业,以生产功能性食品,从而对人类健康和降低疾病风险发挥重要作用。然而,在食品应用中,保持益生菌的活力并将其成功输送到胃肠道仍然是两项具有挑战性的任务。具体来说,本文回顾了益生菌的潜在有益特性,强调了益生菌在食品应用中的使用和挑战以及相关的健康益处。最重要的是,本文还探讨了益生菌增强胃肠道上皮细胞效应的潜在分子机制,包括讨论益生菌细胞表面促进定植的各种表面粘附相关蛋白。
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引用次数: 0
Bifidobacterium lactis Probio-M8 prevents nonalcoholic fatty liver disease in high-fat diet-fed rats: The potential role in modulating gut microbiota 乳双歧杆菌 Probio-M8 可预防高脂饮食喂养大鼠的非酒精性脂肪肝:调节肠道微生物群的潜在作用
Pub Date : 2024-01-21 DOI: 10.1002/fbe2.12076
Wen Fan, Kairui Tang, Yuanjun Deng, Chuiyang Zheng, Maoxing Pan, Dajin Pi, Zheng Liang, Jianwei Zhen, Qinhe Yang, Yupei Zhang

Nonalcoholic fatty liver disease (NAFLD) is a major global health problem with few therapeutic options available so far. Accumulating evidence suggests that probiotics have beneficial effects on NAFLD by modulating gut microbiota. Bifidobacterium lactis Probio-M8 (M8) is a new probiotic strain isolated from human breast milk. The aim of this study was to investigate whether M8 could protect against NAFLD in rats fed a high-fat diet by modulating gut microbiota. In this study, rats were randomly distributed into four groups: normal diet (ND) group, normal diet plus M8 (ND+M8) group, high-fat diet (HFD) group, and high-fat diet plus M8 (HFD+M8) group. Ten weeks later, hepatic morphological changes and biochemical indicators were measured. 16S rDNA sequencing was applied to analyze the gut microbiota alterations. Our results showed that M8 administration effectively improved hepatic steatosis and liver damage in high-fat diet-fed rats. 16S rDNA analysis of gut microbiota indicated that M8 could modulate the gut microbiota composition, especially increasing Bifidobacterium and decreasing Bilophila, Lachnoclostridium, GCA-900066225, and Phascolarctobacterium in high-fat diet-fed rats. In conclusion, our findings demonstrated that M8 could protect against NAFLD in rats fed a high-fat diet, which may be attributed to the modulation of gut microbiota.

非酒精性脂肪肝(NAFLD)是一个重大的全球性健康问题,迄今为止可供选择的治疗方法很少。越来越多的证据表明,益生菌可通过调节肠道微生物群对非酒精性脂肪肝产生有益影响。乳双歧杆菌 Probio-M8 (M8) 是一种从母乳中分离出来的新型益生菌株。本研究旨在探讨 M8 是否能通过调节肠道微生物群来保护以高脂肪饮食喂养的大鼠免受非酒精性脂肪肝的影响。本研究将大鼠随机分为四组:正常饮食(ND)组、正常饮食加 M8(ND+M8)组、高脂饮食(HFD)组和高脂饮食加 M8(HFD+M8)组。十周后,测定肝脏形态学变化和生化指标。16S rDNA测序用于分析肠道微生物群的变化。结果表明,服用 M8 能有效改善高脂饮食大鼠的肝脏脂肪变性和肝损伤。肠道微生物群的 16S rDNA 分析表明,M8 可调节高脂饮食大鼠的肠道微生物群组成,尤其是增加了双歧杆菌,减少了双唇杆菌、Lachnoclostridium、GCA-900066225 和 Phascolarctobacterium。总之,我们的研究结果表明,M8 可保护高脂饮食大鼠免受非酒精性脂肪肝的影响,这可能归因于对肠道微生物群的调节。
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引用次数: 0
Beneficial effect of Lactobacillus fermentum GLF-217 on hyperlipidemia in high-fat diet golden hamsters by regulating gut microbiota 乳酸菌发酵产物 GLF-217 通过调节肠道微生物群对高脂饮食金色仓鼠高脂血症的有益影响
Pub Date : 2023-12-25 DOI: 10.1002/fbe2.12074
Jun Wang, Ruimin Wang, Ao Li, Aijie Liu, Pengfei Luo, Meng Zhan, Xiaoli Zhou, Lihao Chen, Jiachao Zhang, Kun Cai, Dongxue Huo

To investigate the hypolipidemic and modulation effects of probiotics, the golden hamster of hyperlipidemia model was established and intervened with Lactobacillus fermentum GLF-217 (GLF-217) and lovastatin (LOV) for 6 weeks. Body weight and serum biochemical indexes were measured, and liver, colonic contents, and feces were collected after autopsy for pathological and gut microbiota analysis. The results showed that the intervention of GLF-217 and LOV could significantly reduce serum lipid levels and liver fat accumulation in golden hamsters on a high-fat diet, more importantly, the LOV intervention could cause damage to the liver. The LOV intervention significantly reduced the contents of short-chain fatty acids (SCFAs) in the colonic and downregulated the abundance of SCFAs-producing bacteria, but GLF-217 intake increased butyric acid, thus reducing blood lipids and protected the liver. This study elucidates the possible mechanism of the hypolipidemic effect of probiotics in terms of regulating gut microbiota.

为了研究益生菌的降脂和调节作用,建立了高脂血症金色仓鼠模型,并用乳酸杆菌发酵产物GLF-217(GLF-217)和洛伐他汀(LOV)干预6周。测定体重和血清生化指标,解剖后收集肝脏、结肠内容物和粪便进行病理和肠道微生物群分析。结果表明,GLF-217和LOV干预能显著降低高脂饮食金色仓鼠的血清脂质水平和肝脏脂肪堆积,更重要的是,LOV干预会对肝脏造成损伤。LOV干预明显降低了结肠中短链脂肪酸(SCFAs)的含量,并下调了SCFAs产生菌的丰度,但GLF-217的摄入增加了丁酸,从而降低了血脂并保护了肝脏。这项研究从调节肠道微生物群的角度阐明了益生菌降血脂作用的可能机制。
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引用次数: 0
Effect of addition of pullulan on the properties of native/waxy corn starch‐based films 添加拉鲁兰对原生/蜡质玉米淀粉薄膜性能的影响
Pub Date : 2023-12-08 DOI: 10.1002/fbe2.12070
Hao Cheng, Long Chen, Zipei Zhang, Ruojie Zhang, D. Mcclements, Hao Xu, Zhenlin Xu, Man Meng, Zhen Jin
This article reports on the preparation of native/waxy corn starch (NCS/WCS)‐based films with the addition of pullulan (PUL). Our study investigated the effects of varying amounts of PUL and amylose contents on the structure, mechanical and physicochemical properties of corn starch films. Notably, it was observed that WCS films with low amylose content exhibited superior transparency, while NCS films with high amylose content demonstrated enhanced tensile strength (up to 7.35 ± 0.18 MPa). Fourier transform infrared spectroscopy (FTIR) analysis indicated that the addition of PUL did not change the molecular interactions within the corn starch films. The X‐ray diffraction (XRD) results supported that the WCS films were amorphous, while the NCS film exhibited B‐type and V‐type crystals. Moreover, an increase in PUL content led to a gradual reduction in the crystallinity of both WCS films and NCS films. The addition of PUL improved the mechanical properties and oxygen barrier characteristics of these films but had an adverse impact on their water vapor barrier properties. These findings offer valuable insights for the selection of additives for corn starch film, which can further enhance the practical application potential of corn starch films in food and other industries.
本文报道了添加普鲁兰(PUL)制备天然/蜡质玉米淀粉(NCS/WCS)基薄膜。研究了不同用量的PUL和直链淀粉含量对玉米淀粉膜结构、力学和理化性质的影响。值得注意的是,直链淀粉含量低的WCS薄膜具有更好的透明度,而直链淀粉含量高的NCS薄膜具有更高的抗拉强度(高达7.35±0.18 MPa)。傅里叶变换红外光谱(FTIR)分析表明,PUL的加入没有改变玉米淀粉膜内的分子相互作用。X射线衍射(XRD)结果表明WCS薄膜是无定形的,而NCS薄膜则表现为B型和V型晶体。此外,PUL含量的增加导致WCS薄膜和NCS薄膜的结晶度逐渐降低。PUL的加入改善了膜的力学性能和氧阻隔性能,但对膜的水蒸气阻隔性能有不利影响。这些研究结果为玉米淀粉膜添加剂的选择提供了有价值的见解,可以进一步增强玉米淀粉膜在食品等行业的实际应用潜力。
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引用次数: 0
Comparison of yield and properties of banana juice extracted by hot water extraction method and different enzymes and preparation of wine from the extracted juices 比较用热水提取法和不同酶提取香蕉汁的产量和特性,以及用提取的汁液制备葡萄酒
Pub Date : 2023-12-04 DOI: 10.1002/fbe2.12071
Swapna S. Dowerah, Arpita Das, Mohit Singla, N. Sit
The yield and properties of banana juice extracted by enzymatic pretreatments using different enzymes and were compared among themselves and with hot water extraction. The treatments that gave higher yield and better properties of the juices were used for winemaking and the properties of the wines were also compared. A comparison of gas chromatography‐mass spectrometry analysis was also done between the juices and the wines. The various treatments were hot water bath extraction and different enzymes (amylase, cellulase, pectinase, and viscozyme) at two concentrations (0.05% and 0.1%). Juice yield, total soluble sugars, pH, titrable acidity, reducing sugars, and transmittance were analyzed. Best results for juice recovery (46.44 ± 0.50%), total soluble solids (14.8 ± 0.01 °Brix), and transmittance (86.93 ± 2.68%) were achieved using pectinase (0.1%), while viscozyme (0.1%) yielded best results for viscosity (1.49 ± 0.09 mPa.s), pH (5.01 ± 0.05), and acidity (0.277 ± 0.01%). The banana juices from these treatments were then used for wine preparation, and the physicochemical parameters were monitored during a 30‐day storage period. Within 20 days during fermentation, a reduction in total soluble solids from 22 °Brix to 6.1 °Brix was observed. Juice from banana pulp can be successfully extracted using the enzymes pectinase and viscozyme, and wine can be prepared from the enzymatically extracted juices.
比较了不同酶制剂和热水浸提法对香蕉汁提取率和性能的影响。采用产量较高、果汁性能较好的处理方法进行了酿酒,并对所制酒的性能进行了比较。还对果汁和葡萄酒进行了气相色谱-质谱分析比较。采用热水浴浸提和不同酶(淀粉酶、纤维素酶、果胶酶和粘酶)浓度(0.05%和0.1%)处理。分析了果汁得率、总可溶性糖、pH、可滴定酸度、还原糖和透光率。果胶酶(0.1%)对果汁回收率(46.44±0.50%)、总可溶性固形物(14.8±0.01°Brix)和透光率(86.93±2.68%)的测定结果最佳,而粘酶(0.1%)对粘度(1.49±0.09 mPa.s)、pH(5.01±0.05)和酸度(0.277±0.01%)的测定结果最佳。然后将这些处理后的香蕉汁用于制酒,并在30天的储存期内监测其理化参数。在发酵的20天内,总可溶性固形物从22°Brix降低到6.1°Brix。利用果胶酶和粘酶可以成功地提取香蕉果肉中的果汁,并可以用酶提取的果汁制备葡萄酒。
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引用次数: 0
Effects of A‐ and B‐type starch granules on composition, structural, thermal, morphological, and pasting properties of starches from diverse wheat varieties A 型和 B 型淀粉颗粒对不同小麦品种淀粉的成分、结构、热力、形态和糊化性能的影响
Pub Date : 2023-12-04 DOI: 10.1002/fbe2.12068
Rajesh Kumar, Narpinder Singh, B. S. Khatkar
The distribution of A‐ and B‐type‐sized starch granules plays a deciding role in controlling the physicochemical, structural, morphological, and functional attributes of wheat starch. Starches of three Indian wheat varieties, viz. DBW 16, WH 147, and WH 542, were fractionated into A‐ and B‐type starch granules and further evaluated for their influence on various attributes of wheat starches using different analytical tools like X‐ray diffraction, scanning electron microscopy (SEM), differential scanning calorimetry, and rapid viscoanalyzer. SEM revealed that the size of large granules (A‐type) ranged from 12.6 to 36.4 µm and small granules (B‐type) varied from 2.53 to 7.52 µm. The amylose content was significantly higher for A‐type starch ranging from 26.6% to 29.68% than B‐type starch ranging from 19.20% to 22.38%. The highest swelling power was observed for B‐type granules, followed by native and A‐type granules, and similar trend was noticed for water absorption. Pasting viscosities of A‐type granules were higher than B‐type for starches of all wheat varieties. A higher pasting temperature was observed in B‐type starch granules, suggesting more resistance to swell during the heating process. X‐ray diffraction of wheat starches showed A‐type pattern of crystallinity, variety DBW 16 (27%) showed the highest relative crystallinity and intensities of peaks in comparison to varieties WH 147 (23.5%) and WH 542 (22.4% as observed in diffractograms and well supported by Fourier transforms infrared spectroscopy. Fractionated large granules of wheat starches exhibited a higher gelatinization temperature than smaller granules and native starches for all the varieties. It was also observed that A‐type granules had higher onset temperature comparatively, which suggested that high energy is required in gelatinization due to more ordered arrangement of starch molecules.
A型和B型淀粉颗粒的分布对小麦淀粉的理化、结构、形态和功能属性起着决定性的控制作用。采用不同的分析工具,如X射线衍射、扫描电镜、差示扫描量热法和快速粘度分析仪,对3个印度小麦品种dbw16、WH 147和WH 542的淀粉进行了分馏,并进一步评估了它们对小麦淀粉各种属性的影响。扫描电镜显示,大颗粒(A型)的尺寸在12.6 ~ 36.4µm之间,小颗粒(B型)的尺寸在2.53 ~ 7.52µm之间。A型淀粉直链淀粉含量为26.6% ~ 29.68%,显著高于B型淀粉的19.20% ~ 22.38%。B型颗粒的溶胀力最高,其次是天然颗粒和A型颗粒,吸水率也有类似的趋势。所有小麦品种淀粉的A型颗粒黏度均高于B型。在B型淀粉颗粒中观察到较高的糊化温度,表明在加热过程中更耐膨胀。小麦淀粉的X射线衍射表现为A型结晶度,品种dbw16(27%)的相对结晶度和峰强度均高于品种WH 147(23.5%)和WH 542(22.4%),傅里叶变换红外光谱也证实了这一点。各品种分馏大颗粒小麦淀粉的糊化温度均高于小颗粒小麦淀粉和天然小麦淀粉。A型颗粒具有较高的起始温度,这表明由于淀粉分子排列更有序,在糊化过程中需要较高的能量。
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引用次数: 0
期刊
Food Bioengineering
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