Green Carbon最新文献

英文中文

COF-999: A high-performance CO2 adsorbent for direct air capture technology COF-999：用于直接空气捕获技术的高性能CO2吸附剂

Green Carbon

Pub Date : 2025-12-01 DOI: 10.1016/j.greenca.2025.03.004

Zibo Yang , Qingqing Li , Bin Li , Tongan Yan , Shuwen Yu , Zhihua Qiao

引用次数: 0

ZMQ-1 breakthrough: A new milestone in zeolite science & technology ZMQ-1的突破：沸石科技的新里程碑

Green Carbon

Pub Date : 2025-12-01 DOI: 10.1016/j.greenca.2025.06.004

Jean-Pierre Gilson , Ulrich Müller

引用次数: 0

Protein language model empowered the robust ASR-driven PET hydrolase featured with two PET binding motifs 蛋白质语言模型增强了强大的asr驱动PET水解酶，具有两个PET结合基序

Green Carbon

Pub Date : 2025-12-01 DOI: 10.1016/j.greenca.2025.03.005

Yibo Song , Anni Li , Haiyang Cui , Bo Zhou , Jie Qiao , Junnan Wei , Xiujuan Li

Various tools specifically designed to accelerate evolutionary processes for biocatalysis and biotransformation have been developed in the field of protein engineering. Among them, protein language modeling (PLM) is extremely efficient for large-scale screening, thus initiating a new era of accelerated prediction. Therefore, this study considered the highly promising ancestral sequence reconstruction 1(ASR1)-polyethylene terephthalate hydrolase (PETase), previously obtained via ancestral sequence reconstruction, as a representative model. The PLM Evolutionary Scale Modeling-1V was used as an amino acid optimizer to efficiently identify four beneficial variants that improved terephthalic acid (TPA) yield by 1.7-fold. The triple variant ASR1-HRT (N81H/W120R/V265T) showed a 6.1-fold increase in TPA yield compared with that of the five-site variant FAST-PETase (N233K/R224Q/S121E/D186H/R280A) through the recombination of a single beneficial variant. Moreover, ASR1-HRT achieved a depolymerization rate of 96.1% for commercial polyethylene terephthalate (PET) plastics. Molecular dynamics simulations showed that the enhancement of structural stability at high temperatures and changes in catalytic reactions due to solvation contributed to efficient and stable properties. In addition, through exploring the enzyme-PET film interaction landscape at the molecular level, the two motifs of ASR1-PETase were found to play key roles in the catalytic process at the solid-liquid interface. This enhanced the initial adsorption of the enzyme on PET film, thereby enhancing the hydrolysis performance. Overall, the PLM optimization strategy has the potential to be applied to other enzymes, thereby efficiently accelerating protein engineering.

在蛋白质工程领域，专门设计用于加速生物催化和生物转化的进化过程的各种工具已经开发出来。其中，蛋白质语言建模（protein language modeling， PLM）在大规模筛选中具有极高的效率，开启了加速预测的新时代。因此，本研究将之前通过祖先序列重建获得的极具前景的祖先序列重建1(ASR1)-聚对苯二甲酸乙二醇酯水解酶（PETase）作为代表模型。使用PLM进化尺度模型- 1v作为氨基酸优化器，有效地识别出四种有益的变异，将对苯二甲酸（TPA）的产量提高了1.7倍。三位点变异ASR1-HRT （N81H/W120R/V265T）与五位点变异FAST-PETase （N233K/R224Q/S121E/D186H/R280A）相比，通过单一有益变异的重组，TPA产量增加了6.1倍。此外，ASR1-HRT对商用聚对苯二甲酸乙二醇酯（PET）塑料的解聚率达到96.1%。分子动力学模拟表明，高温下结构稳定性的增强和溶剂化引起的催化反应的改变有助于提高其高效稳定的性能。此外，通过在分子水平上探索酶- pet膜相互作用景观，发现ASR1-PETase的两个基序在固液界面催化过程中发挥关键作用。这增强了酶在PET膜上的初始吸附，从而提高了水解性能。总的来说，PLM优化策略有可能应用于其他酶，从而有效地加速蛋白质工程。

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引用次数: 0

Beyond the MOF Nobel: From seeds of science to sustainable future 超越财政部诺贝尔奖：从科学的种子到可持续的未来

Green Carbon

Pub Date : 2025-12-01 DOI: 10.1016/j.greenca.2025.10.002

Ya-Jun Liu, Heqing Jiang, Xuefeng Lu

引用次数: 0

Dual-functional Fe3O4-decorated porous carbon nanosheets for kinetics-enhanced aqueous zinc–iodine batteries 双功能fe3o4修饰多孔碳纳米片用于动力学增强水锌碘电池

Green Carbon

Pub Date : 2025-12-01 DOI: 10.1016/j.greenca.2025.06.005

Dewei Wang , Xiangyu Kong , Zuoshu Wang , Xinyang Zhang , Jie Zhang , Yuhong Chen

Aqueous rechargeable zinc–iodine batteries (ARZIBs) have been highlighted as a favorable solution for energy storage, in view of their sustainability, cost-effectiveness, and safety. Nonetheless, the practical implementation of ARZIBs is challenged by the sluggish iodine-reduction-reaction kinetics as well as the shuttling behavior of the soluble polyiodide substances. Herein, Fe₃O₄ nanoparticles embedded in porous carbon nanosheets (Fe₃O₄ NPs@PCNs) with a large specific surface area (1407.8 m² g⁻¹) are proposed as an iodine host for ARZIBs. This structure not only enhances iodine adsorption but also supports the electrocatalytic reversible conversion of iodine. Both experimental evidence and theoretical analyses highlight that the presence of the Fe₃O₄ nanoparticles can effectively accelerate the iodine-reduction-reaction reversibly, while mitigating the shuttling effect of polyiodide ions. The resultant ARZIBs exhibit a specific capacity of 269.8 mAh g⁻¹ at 0.5 A g⁻¹, with 85% of this capacity being contributed by the discharge platform. Additionally, they demonstrate high rate capabilities, delivering 211.1 mAh g⁻¹ at 20 A g⁻¹, minimal self-discharge, and cycling stability for 15,000 cycles. This performance is attributed to the synergistic effect of the catalytic activity of Fe₃O₄ nanoparticles and physical confinement provided by the carbon framework. The findings of this study illuminate the critical effect of Fe₃O₄ in transforming a nonpolar carbon material into an efficient iodine host, paving the way for the realization of reversible ARZIBs.

鉴于其可持续性、成本效益和安全性，水溶液可充电锌-碘电池（arzib）已成为一种良好的储能解决方案。然而，arzbs的实际实施受到碘还原反应动力学迟缓以及可溶性多碘化物物质穿梭行为的挑战。本文提出，嵌入多孔碳纳米片（Fe3O4 NPs@PCNs）的Fe3O4纳米颗粒具有较大的比表面积（1407.8 m2 g−1），可作为arzbs的碘宿主。这种结构不仅增强了碘的吸附，而且支持碘的电催化可逆转化。实验证据和理论分析都表明，纳米Fe3O4的存在可以有效地加速碘还原反应，同时减轻多碘离子的穿梭效应。所得的arzbs在0.5 a g−1时的比容量为269.8 mAh g−1，其中85%的容量由放电平台贡献。此外，它们还具有高倍率能力，在20a g - 1时可提供211.1 mAh g - 1，自放电最小，循环稳定性为15,000次。这种性能归因于Fe3O4纳米颗粒的催化活性和碳框架提供的物理约束的协同效应。本研究的发现阐明了Fe3O4在将非极性碳材料转化为高效碘宿主中的关键作用，为实现可逆arzib铺平了道路。

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引用次数: 0

Komagataella phaffii: A versatile platform for the production of value-added chemicals Komagataella phaffii：生产增值化学品的多功能平台

Green Carbon

Pub Date : 2025-12-01 DOI: 10.1016/j.greenca.2025.01.004

Qiang Zhou , Jiahui Yang , Wenming Zhang , Wankui Jiang , Jianxiang Chen , Lei Chen , Yujia Jiang , Fengxue Xin

The development of green biomanufacturing technologies centered around advanced microbial cells has emerged as a hot research field in synthetic biology. As known, Komagataella phaffii is an unconventional yeast with unique biological characteristics, which is generally regarded as safe (GRAS). Owing to its wide substrate spectrum, K. phaffii has been widely genetically modified for valuable chemicals such as organic acids, fatty acids and some nature products. Actually, K. phaffii is very well known for the protein expression, and few reviews have systemically addressed the value-added chemicals by K. phaffii. Accordingly, this review will introduce the most advanced development of genetic editing tools of K. phaffii. Recent progresses and bottlenecks in the production of high-value chemicals using K. phaffii will also be summarized. Finally, future perspectives for the utilization of K. phaffii as a chassis cell used for high-value products synthesis will be discussed.

以先进微生物细胞为核心的绿色生物制造技术已成为合成生物学研究的热点。众所周知，法菲Komagataella phaffii是一种非传统的酵母，具有独特的生物学特性，通常被认为是安全的（GRAS）。由于其广泛的底物光谱，菲氏K.已被广泛用于有价值的化学物质，如有机酸、脂肪酸和一些天然产物的基因改造。实际上，phaffii以其蛋白表达而闻名，很少有文献系统地研究了phaffii的增值化学物质。因此，本文将介绍法菲克氏菌基因编辑工具的最新进展。本文还将总结利用菲氏菌生产高价值化学品的最新进展和瓶颈。最后，讨论了利用法菲氏菌作为高价值产品合成的底盘细胞的未来前景。

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引用次数: 0

Benzodifuran-based polymer donors for binary all-polymer solar cells with nearly 18% efficiency 基于苯二呋喃的聚合物给体用于二元全聚合物太阳能电池，效率接近18%

Green Carbon

Pub Date : 2025-12-01 DOI: 10.1016/j.greenca.2025.02.006

Xiao Kang , Jianxiao Wang , Xin Jing , Zhengkun Du , Hongxiang Li , Fuzhen Bi , Mingliang Sun , Xichang Bao , Junhao Chu

Owing to the strong intermolecular interactions and high energy levels of benzodifuran (BDF)-based copolymers, their photovoltaic performance has progressed slowly. Herein, a "strong donor–weak acceptor" design strategy is proposed that can effectively optimize the strong intermolecular interactions of BDF-based polymers by using the steric effect of long alkyl side chains to weaken the dithiophene quinoxaline-based acceptor unit. Two new high-performance polymer donors, PQR16-BFCl and PQR16-BFF, were prepared via copolymerization with BDF unit. The binary all-polymer solar cells fabricated by combining PQR16-BFCl and PQR16-BFF with PY-IT achieved power conversion efficiencies of 17.91% and 17.26%, respectively, along with high fill factors exceeding 75%. Notably, PQR16-BFCl:PY-IT set a new record efficiency for BDF-based all-polymer solar cell binary devices. This study showed that the strong interaction advantage of BDF can be utilized in a weak acceptor unit system, showing prominent advantages in stabilizing the orientation of polymer molecules, alleviating steric hindrance, and reducing aggregation. This study demonstrates the application of BDF-based donor units and will undoubtedly accelerate the development of BDF-based polymer solar cells.

由于苯二呋喃（BDF）基共聚物分子间相互作用强，能级高，其光伏性能进展缓慢。本文提出了一种“强给体-弱受体”设计策略，利用长烷基侧链的空间位效应削弱二噻吩-喹啉基受体单元，有效优化bdf基聚合物的强分子间相互作用。通过与BDF单元的共聚，制备了两种新型高性能聚合物给体PQR16-BFCl和PQR16-BFF。PQR16-BFCl和PQR16-BFF与PY-IT结合制备的二元全聚合物太阳能电池的功率转换效率分别达到17.91%和17.26%，填充系数超过75%。值得注意的是，PQR16-BFCl:PY-IT创造了基于bdf的全聚合物太阳能电池二元器件效率的新记录。本研究表明，BDF的强相互作用优势可以在弱受体单元体系中发挥作用，在稳定聚合物分子取向、减轻位阻、减少聚集等方面具有突出的优势。该研究展示了基于bdf的供体单元的应用，无疑将加速基于bdf的聚合物太阳能电池的发展。

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引用次数: 0

Electroenzymatic CO2 reduction using dual enzymes encapsulated in Zn-MOF-74 锌- mof -74包封双酶电解法还原CO2

Green Carbon

Pub Date : 2025-12-01 DOI: 10.1016/j.greenca.2025.04.001

Lirong Wang , Jialin Zhang , Guanhua Liu , Hao Zhao , Mimi Chen , Liya Zhou , Ying He , Li Ma , Yunting Liu , Yanjun Jiang

The capture and conversion of CO₂ to valuable products has garnered significant attention. The electroenzymatic reduction of CO₂ is of particular interest owing to its high selectivity and yield. However, this process is hindered by various challenges, such as the low CO₂ solubility in water and the requirement for efficient cofactor regeneration. In this work, we developed a green, water-based, room-temperature synthesis of Zn-MOF-74 for enzyme immobilization. Formate dehydrogenase (FDH) and carbonic anhydrase (CA) are co-encapsulated within the Zn-MOF-74 framework to create a dual enzyme cascade system. This strategy effectively enhances enzyme proximity, stability, and reusability to subsequently promote the enzyme cascade reaction efficiency. Additionally, the adsorption of CO₂ by CA improves the solubility of CO₂ in water. NADH regeneration is achieved using an electron mediator, 2-hydroxy-1-naphthoquinone (HNQ). The optimized electroenzymatic system achieved a formate yield of 3.01 mM within 3 h, which was 4.98 times higher than that of the pristine free enzyme system and 2.27 times higher than the free enzyme system with NADH regeneration.

二氧化碳的捕获和转化为有价值的产品已经引起了极大的关注。由于其高选择性和产率，电酶对CO2的还原是特别感兴趣的。然而，这一过程受到各种挑战的阻碍，例如CO2在水中的溶解度低以及对高效辅因子再生的要求。在这项工作中，我们开发了一种绿色，水基，室温合成用于酶固定化的Zn-MOF-74。甲酸脱氢酶（FDH）和碳酸酐酶（CA）被包裹在Zn-MOF-74框架内，形成双酶级联体系。该策略有效地提高了酶的接近性、稳定性和可重用性，从而提高酶级联反应的效率。此外，CA对CO2的吸附提高了CO2在水中的溶解度。NADH再生是通过电子介质2-羟基-1-萘醌（HNQ）实现的。优化后的电酶体系在3 h内甲酸得率为3.01 mM，比原始游离酶体系高4.98倍，比NADH再生的游离酶体系高2.27倍。

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引用次数: 0

Hydrogen-oxidizing bacteria: A promising contributor to environmental sustainability and resource recycling 氧化氢细菌：环境可持续性和资源循环利用的有前途的贡献者

Green Carbon

Pub Date : 2025-12-01 DOI: 10.1016/j.greenca.2025.02.004

Rui Li , Yufeng Jiang , Kai Luo , Boyang Chen , Haotian Wang , Xiaolei Fan , Junting Pan , Yuri V. Litti , Shanfei Fu , Rongbo Guo

Single-cell proteins (SCPs) derived from hydrogen-oxidizing bacteria (HOB) are a promising strategy to address global food challenges. As versatile microorganisms with rich carbon metabolism, they also have the potential to address environmental emission issues. HOB efficiently utilize carbon through the Calvin-Benson-Bassham (CBB) cycle under autotrophic conditions, introducing an innovative approach to industrial exhaust treatment. In heterotrophic environments, HOB rapidly degrade organic pollutants, including organic carbon, to meet emission standards. Their nitrogen-fixing capacity further supports the production of sustainable nitrogen fertilizers and enables greener agricultural practices. This study systematically reviews the key metabolic pathways of HOB and summarizes their applications in waste gas treatment, organic pollutant purification, and ammonia production. The potential of HOB as drivers of sustainable carbon and nitrogen cycling has been confirmed, offering broader solutions to various environmental and food issues.

从氧化氢细菌（HOB）中提取的单细胞蛋白（SCPs）是解决全球食品挑战的一种有前途的策略。作为具有丰富碳代谢的多功能微生物，它们也具有解决环境排放问题的潜力。HOB在自养条件下通过Calvin-Benson-Bassham （CBB）循环有效利用碳，为工业废气处理引入了一种创新方法。在异养环境中，HOB能快速降解有机污染物，包括有机碳，达到排放标准。它们的固氮能力进一步支持可持续氮肥的生产，并实现更绿色的农业实践。本文系统综述了HOB的主要代谢途径，并对其在废气处理、有机污染物净化、制氨等方面的应用进行了综述。HOB作为可持续碳和氮循环驱动因素的潜力已得到证实，为各种环境和食品问题提供了更广泛的解决方案。

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引用次数: 0

Self-biodegradable polylactide plastic with embedded engineered enzyme 嵌入工程酶的自生物降解聚乳酸塑料

Green Carbon

Pub Date : 2025-12-01 DOI: 10.1016/j.greenca.2025.04.002

Gaofei Zheng , Lijing Han , Junjia Bian , Huiliang Zhang , Xiangguang Yang

引用次数: 0

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