Objective To observe effect of Trim28 (ser473) phosphorylation on viability of hepatocellular carcinoma (HCC) cells during DNA damage. Methods To divide Hepg2 cells into 2 groups as SB203580+ UV group and UV group. Hepg2 cells were modeled for DNA damage by UV (Ultraviolet) irradiation. SB203580+ UV group was treated with Trim28 phosphorylation inhibitor SB203580 after UV irradiation. Expression of Trim28 and phosphorylation-Trim28 ser473 (P-Trim28 ser473)were detected by westernblot experiment. The viability of Hepg2 cells were observed by methyl thiazol tetrazolium (MTT) experiment. Results Compared with UV group (0.94±0.13), the expression value of Trim28 in SB203580+ UV group had no significant difference (t=-0.190, P>0.05). Value of phosphorylation-Trim28 in SB203580+ UV group (0.32±0.04) was obviously lower than UV group (0.72±0.05), the difference had statistical meaning (t=8.500, P<0.05); The 24, 48 and 72 hours after UV irradiation, the cell survive rate of UV group [(95±7)%, (62±10)%, (35±6)%] is higher than SB203580+ UV group [(85±7)%, (37±4)%, (20±3)%]. The difference has statistical meaning (t=-2.326, -5.212, -4.577, P<0.05). Conclusion The viability of HCC cells during DNA damaged can be reduced by inhibition for Trim28 phosphorylation. Key words: Trim28; Phosphorylation; Cell survival
{"title":"Effect of trim28 (ser473) phosphorylation on viability of hepatocellular carcinoma cells during DNA damage","authors":"Yongliang Wang, S. Zhai, Bailin Wang, Wei Yuan, Pengzhen Wang, Xu Zhang, Weilu Jia, Hui Yuan","doi":"10.3760/CMA.J.ISSN.1001-9030.2019.12.018","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.1001-9030.2019.12.018","url":null,"abstract":"Objective \u0000To observe effect of Trim28 (ser473) phosphorylation on viability of hepatocellular carcinoma (HCC) cells during DNA damage. \u0000 \u0000 \u0000Methods \u0000To divide Hepg2 cells into 2 groups as SB203580+ UV group and UV group. Hepg2 cells were modeled for DNA damage by UV (Ultraviolet) irradiation. SB203580+ UV group was treated with Trim28 phosphorylation inhibitor SB203580 after UV irradiation. Expression of Trim28 and phosphorylation-Trim28 ser473 (P-Trim28 ser473)were detected by westernblot experiment. The viability of Hepg2 cells were observed by methyl thiazol tetrazolium (MTT) experiment. \u0000 \u0000 \u0000Results \u0000Compared with UV group (0.94±0.13), the expression value of Trim28 in SB203580+ UV group had no significant difference (t=-0.190, P>0.05). Value of phosphorylation-Trim28 in SB203580+ UV group (0.32±0.04) was obviously lower than UV group (0.72±0.05), the difference had statistical meaning (t=8.500, P<0.05); The 24, 48 and 72 hours after UV irradiation, the cell survive rate of UV group [(95±7)%, (62±10)%, (35±6)%] is higher than SB203580+ UV group [(85±7)%, (37±4)%, (20±3)%]. The difference has statistical meaning (t=-2.326, -5.212, -4.577, P<0.05). \u0000 \u0000 \u0000Conclusion \u0000The viability of HCC cells during DNA damaged can be reduced by inhibition for Trim28 phosphorylation. \u0000 \u0000 \u0000Key words: \u0000Trim28; Phosphorylation; Cell survival","PeriodicalId":10065,"journal":{"name":"中华实验外科杂志","volume":"36 1","pages":"2187-2189"},"PeriodicalIF":0.0,"publicationDate":"2019-12-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43109821","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-12-08DOI: 10.3760/CMA.J.ISSN.1001-9030.2019.12.050
L. Jian, He Rongzhen, Qiu Huibin, Yang Chunxi
Objective To investigate the mechanism of expression and significance of vascular endothelial growth factor and p53 in degenerate intervertebral disc tissue. Methods The nucleus pulposus tissues of 56 patients with lumbar disc herniation were collected as degeneration group and 20 patients with lumbar burst fracture as normal group. The nucleus pulposus was divided into five grades according to Thompson classification. We evaluated the expression of vascular endothelial growth factor and p53 in 56 pathological sections of intervertebral disc tissues with immunohistochemical method. Then, we recorded and graded positive staining rates of vascular endothelial growth factor and p53, respectively. T-test was used to compare the positive rate of vascular endothelial growth factor and p53 in degenerative group and normal group. Spearman grade correlation analysis was used to analyze the correlation between the grade of intervertebral disc degeneration and the expression of vascular endothelial growth factor and p53. Results We found 38 cases (67.86%) with vascular infiltration of the degenerative group. The positive rates of vascular endothelial growth factor and p53 staining in degenerative group were 71.43% (40/56) and 60.71% (34/56), respectively. The total expression rate was 55.36% (31/56), and the difference was significant (P<0.05). There was no positive expression of vascular endothelial growth factor and p53 in 20 normal intervertebral disc tissues in normal group. The positive rates of vascular endothelial growth factor and p53 staining were 84.21% (32/38) and 63.16% (24/38) in the degenerative disc tissues with vascular infiltration, and 44.44% (8/18) and 55.56% (10/18) in the degenerative disc tissues without vascular infiltration, respectively. The differences were statistically significant (P<0.05). The expression rates of vascular endothelial growth factor and p53 in vascular invasive tissues were significantly higher than those in non-invasive tissues. Degenerative grade of intervertebral disc was positively correlated with the expression of vascular endothelial growth factor and p53. Conclusion Vascular endothelial growth factor and p53 gene are co-expressed in degenerated intervertebral disc tissue, which act on the formation and infiltration of new blood vessels and accelerate the degeneration of intervertebral disc tissue. Key words: Vascular endothelial growth factor; P53; Degenerated intervertebral disc tissues; Vascular infiltration
{"title":"Expression and significance of vascular endothelial growth factor and p53 in degenerated intervertebral disc tissues","authors":"L. Jian, He Rongzhen, Qiu Huibin, Yang Chunxi","doi":"10.3760/CMA.J.ISSN.1001-9030.2019.12.050","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.1001-9030.2019.12.050","url":null,"abstract":"Objective \u0000To investigate the mechanism of expression and significance of vascular endothelial growth factor and p53 in degenerate intervertebral disc tissue. \u0000 \u0000 \u0000Methods \u0000The nucleus pulposus tissues of 56 patients with lumbar disc herniation were collected as degeneration group and 20 patients with lumbar burst fracture as normal group. The nucleus pulposus was divided into five grades according to Thompson classification. We evaluated the expression of vascular endothelial growth factor and p53 in 56 pathological sections of intervertebral disc tissues with immunohistochemical method. Then, we recorded and graded positive staining rates of vascular endothelial growth factor and p53, respectively. T-test was used to compare the positive rate of vascular endothelial growth factor and p53 in degenerative group and normal group. Spearman grade correlation analysis was used to analyze the correlation between the grade of intervertebral disc degeneration and the expression of vascular endothelial growth factor and p53. \u0000 \u0000 \u0000Results \u0000We found 38 cases (67.86%) with vascular infiltration of the degenerative group. The positive rates of vascular endothelial growth factor and p53 staining in degenerative group were 71.43% (40/56) and 60.71% (34/56), respectively. The total expression rate was 55.36% (31/56), and the difference was significant (P<0.05). There was no positive expression of vascular endothelial growth factor and p53 in 20 normal intervertebral disc tissues in normal group. The positive rates of vascular endothelial growth factor and p53 staining were 84.21% (32/38) and 63.16% (24/38) in the degenerative disc tissues with vascular infiltration, and 44.44% (8/18) and 55.56% (10/18) in the degenerative disc tissues without vascular infiltration, respectively. The differences were statistically significant (P<0.05). The expression rates of vascular endothelial growth factor and p53 in vascular invasive tissues were significantly higher than those in non-invasive tissues. Degenerative grade of intervertebral disc was positively correlated with the expression of vascular endothelial growth factor and p53. \u0000 \u0000 \u0000Conclusion \u0000Vascular endothelial growth factor and p53 gene are co-expressed in degenerated intervertebral disc tissue, which act on the formation and infiltration of new blood vessels and accelerate the degeneration of intervertebral disc tissue. \u0000 \u0000 \u0000Key words: \u0000Vascular endothelial growth factor; P53; Degenerated intervertebral disc tissues; Vascular infiltration","PeriodicalId":10065,"journal":{"name":"中华实验外科杂志","volume":"36 1","pages":"2289-2291"},"PeriodicalIF":0.0,"publicationDate":"2019-12-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48854454","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-12-08DOI: 10.3760/CMA.J.ISSN.1001-9030.2019.12.036
Xin Zhou, Shaowei Wang, Yan-Fei Yang, Wenjie Niu, Haoran Liang, Jiangong Lu, Kang Wang, L. Pengcui, Xiaochun Wei
Objective To explore the role and mechanism of indian hedgehog (Ihh) in degenerative and abnormal calcification of chondrocyte. Methods Costal chondrocytes were extracted from C57 mice 6 days after birth, By HE staining, Saffron o solid green staining, collagen type Ⅱ immunohistochemical to identify the chondrocyte phenotype The experiment was divided into the ihh high expression group, and Ihh recombinant protein 5 mg/L was added. Ihh knockout group was added to cyclopamine 20 μmol/L. In the blank control group, phosphate buffer solution (PBS) was added. Using real-time quantitative polymerase chain reaction (Real-time PCR) to detect typeⅡcollagen (COLⅡ), type X collagen (COLX), Runt related transcription factor (Runx2), osteocalcin (OCN), alkaline phosphatase (ALP), aggrecan (AGG), progressive ankylosis (ANKH), extracellular nucleotide phosphatase 1 (ENPP1) factors mRNA expression level. Flow cytometry was used to detect the effect of inhibition of Ihh signaling pathway on chondrocyte apoptosis. Results Compared with the control group, the expression of ALP (3.450±1.357 vs. 1.223±0.740), OCN (2.410±0.395 vs. 1.093±0.453), Runx2 (3.057±1.477 vs. 1.144±0.574) and COLX (3.804±1.400 vs. 1.116±0.511) was increased in the high expression group, and the expression of ANKH (0.255±0.042 vs. 1.101±0.471), AGG (0.574±0.355 vs. 1.007±0.126) and COLII (0.670±0.065 vs. 1.027±0.236), ENPP1 (0.354±0.058 vs. 1.091±0.446) was decreased (P<0.05). When the cyclopamine is added, the opposite result appears(P<0.05). Flow cytometry results showed that Inhibition of Ihh signaling pathway can induce apoptosis (t=9.412, P<0.05). Conclusion Ihh can raise COLX, Runx2, OCN and ALP, inhibit the ANKH, ENPP1, AGG and COLⅡ gene expression, and further promote the cartilage cells anomaly calcified. Key words: Indian hedgehog; Chondrocytes; Calcification
{"title":"Research on the role and mechanism of indian hedgehog protein in abnormal calcification degeneration of chondrocytes","authors":"Xin Zhou, Shaowei Wang, Yan-Fei Yang, Wenjie Niu, Haoran Liang, Jiangong Lu, Kang Wang, L. Pengcui, Xiaochun Wei","doi":"10.3760/CMA.J.ISSN.1001-9030.2019.12.036","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.1001-9030.2019.12.036","url":null,"abstract":"Objective \u0000To explore the role and mechanism of indian hedgehog (Ihh) in degenerative and abnormal calcification of chondrocyte. \u0000 \u0000 \u0000Methods \u0000Costal chondrocytes were extracted from C57 mice 6 days after birth, By HE staining, Saffron o solid green staining, collagen type Ⅱ immunohistochemical to identify the chondrocyte phenotype The experiment was divided into the ihh high expression group, and Ihh recombinant protein 5 mg/L was added. Ihh knockout group was added to cyclopamine 20 μmol/L. In the blank control group, phosphate buffer solution (PBS) was added. Using real-time quantitative polymerase chain reaction (Real-time PCR) to detect typeⅡcollagen (COLⅡ), type X collagen (COLX), Runt related transcription factor (Runx2), osteocalcin (OCN), alkaline phosphatase (ALP), aggrecan (AGG), progressive ankylosis (ANKH), extracellular nucleotide phosphatase 1 (ENPP1) factors mRNA expression level. Flow cytometry was used to detect the effect of inhibition of Ihh signaling pathway on chondrocyte apoptosis. \u0000 \u0000 \u0000Results \u0000Compared with the control group, the expression of ALP (3.450±1.357 vs. 1.223±0.740), OCN (2.410±0.395 vs. 1.093±0.453), Runx2 (3.057±1.477 vs. 1.144±0.574) and COLX (3.804±1.400 vs. 1.116±0.511) was increased in the high expression group, and the expression of ANKH (0.255±0.042 vs. 1.101±0.471), AGG (0.574±0.355 vs. 1.007±0.126) and COLII (0.670±0.065 vs. 1.027±0.236), ENPP1 (0.354±0.058 vs. 1.091±0.446) was decreased (P<0.05). When the cyclopamine is added, the opposite result appears(P<0.05). Flow cytometry results showed that Inhibition of Ihh signaling pathway can induce apoptosis (t=9.412, P<0.05). \u0000 \u0000 \u0000Conclusion \u0000Ihh can raise COLX, Runx2, OCN and ALP, inhibit the ANKH, ENPP1, AGG and COLⅡ gene expression, and further promote the cartilage cells anomaly calcified. \u0000 \u0000 \u0000Key words: \u0000Indian hedgehog; Chondrocytes; Calcification","PeriodicalId":10065,"journal":{"name":"中华实验外科杂志","volume":"36 1","pages":"2244-2246"},"PeriodicalIF":0.0,"publicationDate":"2019-12-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45531485","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-12-08DOI: 10.3760/CMA.J.ISSN.1001-9030.2019.12.027
Zi Ye, Baojun Ma, Lei Wang, Yi Zhang, Y. Mao
Objective To investigate the activation of microglia in state of continuity (SE) of epileptic rat model and the temporal and spatial expression of different types of microglia. Methods 60 healthy male Wistar rats were randomly divided into control group, SE at the beginning (NC group), SE lasting for 30 minutes (SE30min group), SE lasting for 1 hour (SE1h group), SE holding. For 2 hours (SE2h), 12 rats in each group. Behavioral changes and activation of microglia in hippocampus were observed. The expression levels of IL-6, IL-1beta and IL-10 and IL-4 were detected. Results In the SE group, with the prolongation of time, the response of MG cells increased, and the activation of MG cells was most obvious in SE2h group. The expression levels of IL-10, IL-4 protein and mRNA in SE1h group and SE2h group were significantly higher than those in NC group and SE30min group, while the expression levels of IL-6, IL-1beta protein and mRNA in M1 group were significantly lower than those in NC group and SE30min group (P<0.05). Conclusion Activation of microglia can be seen in pilocarpine-induced epilepsy rat model. With the prolongation of seizure time, polarization of microglia gradually transforms into neurotrophic M2 cells. The expression levels of anti-inflammatory factors IL-10 and IL-4 will increase, while the expression of pro-inflammatory factors IL-6 and IL-1beta will decrease. Key words: Status epilepticus; Microglia; Cell polarization; Classical activation; Alternative activation
{"title":"Activation of microglia in rat model of epilepsy during persistent state and analysis of temporal and spatial expression of different types of microglia","authors":"Zi Ye, Baojun Ma, Lei Wang, Yi Zhang, Y. Mao","doi":"10.3760/CMA.J.ISSN.1001-9030.2019.12.027","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.1001-9030.2019.12.027","url":null,"abstract":"Objective \u0000To investigate the activation of microglia in state of continuity (SE) of epileptic rat model and the temporal and spatial expression of different types of microglia. \u0000 \u0000 \u0000Methods \u000060 healthy male Wistar rats were randomly divided into control group, SE at the beginning (NC group), SE lasting for 30 minutes (SE30min group), SE lasting for 1 hour (SE1h group), SE holding. For 2 hours (SE2h), 12 rats in each group. Behavioral changes and activation of microglia in hippocampus were observed. The expression levels of IL-6, IL-1beta and IL-10 and IL-4 were detected. \u0000 \u0000 \u0000Results \u0000In the SE group, with the prolongation of time, the response of MG cells increased, and the activation of MG cells was most obvious in SE2h group. The expression levels of IL-10, IL-4 protein and mRNA in SE1h group and SE2h group were significantly higher than those in NC group and SE30min group, while the expression levels of IL-6, IL-1beta protein and mRNA in M1 group were significantly lower than those in NC group and SE30min group (P<0.05). \u0000 \u0000 \u0000Conclusion \u0000Activation of microglia can be seen in pilocarpine-induced epilepsy rat model. With the prolongation of seizure time, polarization of microglia gradually transforms into neurotrophic M2 cells. The expression levels of anti-inflammatory factors IL-10 and IL-4 will increase, while the expression of pro-inflammatory factors IL-6 and IL-1beta will decrease. \u0000 \u0000 \u0000Key words: \u0000Status epilepticus; Microglia; Cell polarization; Classical activation; Alternative activation","PeriodicalId":10065,"journal":{"name":"中华实验外科杂志","volume":"36 1","pages":"2214-2216"},"PeriodicalIF":0.0,"publicationDate":"2019-12-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45805539","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-12-08DOI: 10.3760/CMA.J.ISSN.1001-9030.2019.12.007
Dawei Zhu, Jun Feng, Lujun Chen, You-sheng Zhou, Qi Wang
Objective To investigate the clinical significance and biological function of human endogenous retrovirus subfamily H long terminal repeat associating protein 2 (HHLA2) in human clear cell renal cell carcinoma (ccRCC). Methods Down-regulation of HHLA2 was performed by using RNA interference (RNAi) method to investigate the role of HHLA2 in regulation of biological behaviors in human clear cell renal cell carcinoma cell lines 786-O and ACHN. The cell counting kit-8 (CCK-8) assay, wound healing assay, and transwell invasion assay were used to examine the cellular function after HHLA2 knockdown of these cells. We also identified the differentially expressed genes upon HHLA2 knockdown in ccRCC cell lines by using gene microarray analysis. Results To investigate the functions of HHLA2 in human ccRCC, we successfully constructed HHLA2 knockdown expression in human ccRCC cell lines by using the RNAi method. CCK-8 assay showed that decreased HHLA2 expression in ccRCC cell lines 786-O and ACHN significantly attenuated cell proliferation. The wound healing assay showed that decreased HHLA2 expression in ccRCC cell lines 786-O and ACHN significantly attenuated cell migration (LV-HHLA2-sh1: 786-O: F=99.340, P<0.01, ACHN: F=113.500, P<0.01; LV-HHLA2-sh2: 786-O: F=35.320, P<0.01, ACHN: F=26.470, P<0.01). Transwell invasion assay showed that decreased HHLA2 expression in ccRCC cell lines 786-O and ACHN significantly attenuated cell invasion. The cell cycle arrest at G1 phase was induced and the expressions of Cyclin D1, c-Myc and Cyclin E1 were decreased. In addition, according to the microarray data, the expressions of epithelia-to-mesenchymal transition markers, such as E-cadherin, N-cadherin and Vimentin, were significantly changed after knockdown of HHLA2 expression. After knockdown of HHLA2 in 786-O and ACHN, the expression of E-cadherin was significantly increased, meanwhile the expression of N-cadherin and Vimentin were significantly decreased. Conclusion Our findings indicated that HHLA2 was involved in the progression of human ccRCC and could be used as an important prognostic predictor for this malignancy. Key words: Human endogenous retrovirus subfamily H long terminal repeat associating protein 2; Human clear cell renal cell carcinoma; RNA interference
{"title":"Human endogenous retrovirus subfamily H long terminal repeat associating protein 2 expression and its contribution to the regulation of biological function of human clear cell renal cell carcinoma cell line","authors":"Dawei Zhu, Jun Feng, Lujun Chen, You-sheng Zhou, Qi Wang","doi":"10.3760/CMA.J.ISSN.1001-9030.2019.12.007","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.1001-9030.2019.12.007","url":null,"abstract":"Objective \u0000To investigate the clinical significance and biological function of human endogenous retrovirus subfamily H long terminal repeat associating protein 2 (HHLA2) in human clear cell renal cell carcinoma (ccRCC). \u0000 \u0000 \u0000Methods \u0000Down-regulation of HHLA2 was performed by using RNA interference (RNAi) method to investigate the role of HHLA2 in regulation of biological behaviors in human clear cell renal cell carcinoma cell lines 786-O and ACHN. The cell counting kit-8 (CCK-8) assay, wound healing assay, and transwell invasion assay were used to examine the cellular function after HHLA2 knockdown of these cells. We also identified the differentially expressed genes upon HHLA2 knockdown in ccRCC cell lines by using gene microarray analysis. \u0000 \u0000 \u0000Results \u0000To investigate the functions of HHLA2 in human ccRCC, we successfully constructed HHLA2 knockdown expression in human ccRCC cell lines by using the RNAi method. CCK-8 assay showed that decreased HHLA2 expression in ccRCC cell lines 786-O and ACHN significantly attenuated cell proliferation. The wound healing assay showed that decreased HHLA2 expression in ccRCC cell lines 786-O and ACHN significantly attenuated cell migration (LV-HHLA2-sh1: 786-O: F=99.340, P<0.01, ACHN: F=113.500, P<0.01; LV-HHLA2-sh2: 786-O: F=35.320, P<0.01, ACHN: F=26.470, P<0.01). Transwell invasion assay showed that decreased HHLA2 expression in ccRCC cell lines 786-O and ACHN significantly attenuated cell invasion. The cell cycle arrest at G1 phase was induced and the expressions of Cyclin D1, c-Myc and Cyclin E1 were decreased. In addition, according to the microarray data, the expressions of epithelia-to-mesenchymal transition markers, such as E-cadherin, N-cadherin and Vimentin, were significantly changed after knockdown of HHLA2 expression. After knockdown of HHLA2 in 786-O and ACHN, the expression of E-cadherin was significantly increased, meanwhile the expression of N-cadherin and Vimentin were significantly decreased. \u0000 \u0000 \u0000Conclusion \u0000Our findings indicated that HHLA2 was involved in the progression of human ccRCC and could be used as an important prognostic predictor for this malignancy. \u0000 \u0000 \u0000Key words: \u0000Human endogenous retrovirus subfamily H long terminal repeat associating protein 2; Human clear cell renal cell carcinoma; RNA interference","PeriodicalId":10065,"journal":{"name":"中华实验外科杂志","volume":"36 1","pages":"2151-2153"},"PeriodicalIF":0.0,"publicationDate":"2019-12-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46232121","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Establishment of a model of gastrointestinal dilatation in mice","authors":"Yanling Ma, Dan Wang, Xiaolong Liu, Xueyan Wang, Bofang Wang, Zedong Feng, Hao Chen","doi":"10.3760/CMA.J.ISSN.1001-9030.2019.12.024","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.1001-9030.2019.12.024","url":null,"abstract":"","PeriodicalId":10065,"journal":{"name":"中华实验外科杂志","volume":"36 1","pages":"2206-2206"},"PeriodicalIF":0.0,"publicationDate":"2019-12-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47838424","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-12-08DOI: 10.3760/CMA.J.ISSN.1001-9030.2019.12.048
Wei Li, Hefei Li, Q. Gong, Duo Zhang, Yan Nan
Objective To investigate the consistency of plasma plasma circulating tumor deoxyribonucleic acid (ctDNA) and tumor tissue samples in the mutation detection in non-small cell lung cancer (NSCLC) patients and its relationship with prognosis. Methods 50 NSCLC patients in our hospital from March 2017 to June 2018 were selected, plasma ctDNA and tumor tissue DNA were extracted, and the mutations of epidermal growth factor receptor (EGFR) gene were detected by amplification refractory mutation system (ARMS) method, and the consistency of the two methods was analyzed. Then the correlation between EGFR gene mutation detected by plasma ctDNA and progression-free survival (PFS) was analyzed. Results The mutation rate of EGFR gene detected by plasma ctDNA in female and non-smoking patients was significantly higher than that in male and smoking patients (P<0.05). The specificity, sensitivity, positive predictive value and negative predictive value of detection of EGFR gene mutation by ctDNA in plasma were 100.00%, 58.82%, 53.33% and 100.00%, respectively. The consistency of ctDNA and tumor tissue in the detection of EGFR gene mutation was good (P<0.05). The plasma ctDNA and tumor tissues showed that the PFS of EGFR mutant patients was significantly longer than that of wild type patients(P<0.05). Conclusion Plasma ctDNA and tumor tissue samples of NSCLC patients have a good consistency in detecting EGFR gene mutation, and plasma ctDNA has a certain predictive value for the prognosis of patients undergoing chemotherapy, which can be used as a laboratory method for clinical detection of EGFR gene mutation and prognosis assessment. Key words: Non-small cell lung cancer; Circulating tumor deoxyribonucleic acid; Epidermal growth factor receptor gene; Gene mutation; Prognosis
{"title":"Consistency of plasma circulating tumor deoxyribonucleic acid and tumor tissue samples in the mutation detection in non-small cell lung cancer patients and its relationship with prognosis","authors":"Wei Li, Hefei Li, Q. Gong, Duo Zhang, Yan Nan","doi":"10.3760/CMA.J.ISSN.1001-9030.2019.12.048","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.1001-9030.2019.12.048","url":null,"abstract":"Objective \u0000To investigate the consistency of plasma plasma circulating tumor deoxyribonucleic acid (ctDNA) and tumor tissue samples in the mutation detection in non-small cell lung cancer (NSCLC) patients and its relationship with prognosis. \u0000 \u0000 \u0000Methods \u000050 NSCLC patients in our hospital from March 2017 to June 2018 were selected, plasma ctDNA and tumor tissue DNA were extracted, and the mutations of epidermal growth factor receptor (EGFR) gene were detected by amplification refractory mutation system (ARMS) method, and the consistency of the two methods was analyzed. Then the correlation between EGFR gene mutation detected by plasma ctDNA and progression-free survival (PFS) was analyzed. \u0000 \u0000 \u0000Results \u0000The mutation rate of EGFR gene detected by plasma ctDNA in female and non-smoking patients was significantly higher than that in male and smoking patients (P<0.05). The specificity, sensitivity, positive predictive value and negative predictive value of detection of EGFR gene mutation by ctDNA in plasma were 100.00%, 58.82%, 53.33% and 100.00%, respectively. The consistency of ctDNA and tumor tissue in the detection of EGFR gene mutation was good (P<0.05). The plasma ctDNA and tumor tissues showed that the PFS of EGFR mutant patients was significantly longer than that of wild type patients(P<0.05). \u0000 \u0000 \u0000Conclusion \u0000Plasma ctDNA and tumor tissue samples of NSCLC patients have a good consistency in detecting EGFR gene mutation, and plasma ctDNA has a certain predictive value for the prognosis of patients undergoing chemotherapy, which can be used as a laboratory method for clinical detection of EGFR gene mutation and prognosis assessment. \u0000 \u0000 \u0000Key words: \u0000Non-small cell lung cancer; Circulating tumor deoxyribonucleic acid; Epidermal growth factor receptor gene; Gene mutation; Prognosis","PeriodicalId":10065,"journal":{"name":"中华实验外科杂志","volume":"36 1","pages":"2283-2285"},"PeriodicalIF":0.0,"publicationDate":"2019-12-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44121510","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-12-08DOI: 10.3760/CMA.J.ISSN.1001-9030.2019.12.047
Jinlan Lin, Tianxing Guo, Xiaojie Pan
Objective To explored the clinical value of preoperative fibrinogen and neutrophil to lymphocyte ratio (NLR) in patients with esophageal squamous cell carcinoma (ESCC) in this study. Methods 327 patients undergoing ESCC radical surgery was performed to evaluate prognostic factors for overall survival (OS) by univariate and multivariate COX regression analyses. The optimal cut-off values for fibrinogen and NLR were 3.09 g/L and 1.89, respectively. The fibrinogen and NLR (F-NLR) scoring criteria were defined according to the cut-off criteria. High fibrinogen (≥3.09 g/L) and high NLR (≥1.89) were defined as 2 points, patients with high fibrinogen or high NLR were defined as 1 point, and neither hyperfibrinogen or high NLR scored 0 points. Results The OS of patients with high fibrinogen ESCC was significantly poor than patients with low fibrinogen (31.4% vs. 63.3%, P<0.01), while the OS of patients with elevated NLR was also significantly poor than patients with low NLR (40.4% vs. 50.3%). The F-NLR score was significantly correlated with tumor size (P<0.01) and pathological stage (P<0.01). The 5-year OS rates for F-NLR score groups 0, 1, and 2 were 69.1%, 42.6%, and 31.9%, respectively, there were significant differences between subgroups (P<0.01). Multivariate analysis showed that tumor size (P<0.01), pathological stage (P<0.01), and F-NLR (P<0.01) were independent prognostic factors for OS. Conclusion Preoperative F-NLR score can be used as an independent prognostic indicator for ESCC patients. Key words: Esophageal squamous cell carcinoma; Fibrinogen; Platelet to lymphocyte ratio; Prognostic indicator
{"title":"Combination of preoperative fibrinogen and neutrophil to lymphocyte ratio is a predictive prognostic factor in esophageal squamous cell carcinoma systematic review","authors":"Jinlan Lin, Tianxing Guo, Xiaojie Pan","doi":"10.3760/CMA.J.ISSN.1001-9030.2019.12.047","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.1001-9030.2019.12.047","url":null,"abstract":"Objective \u0000To explored the clinical value of preoperative fibrinogen and neutrophil to lymphocyte ratio (NLR) in patients with esophageal squamous cell carcinoma (ESCC) in this study. \u0000 \u0000 \u0000Methods \u0000327 patients undergoing ESCC radical surgery was performed to evaluate prognostic factors for overall survival (OS) by univariate and multivariate COX regression analyses. The optimal cut-off values for fibrinogen and NLR were 3.09 g/L and 1.89, respectively. The fibrinogen and NLR (F-NLR) scoring criteria were defined according to the cut-off criteria. High fibrinogen (≥3.09 g/L) and high NLR (≥1.89) were defined as 2 points, patients with high fibrinogen or high NLR were defined as 1 point, and neither hyperfibrinogen or high NLR scored 0 points. \u0000 \u0000 \u0000Results \u0000The OS of patients with high fibrinogen ESCC was significantly poor than patients with low fibrinogen (31.4% vs. 63.3%, P<0.01), while the OS of patients with elevated NLR was also significantly poor than patients with low NLR (40.4% vs. 50.3%). The F-NLR score was significantly correlated with tumor size (P<0.01) and pathological stage (P<0.01). The 5-year OS rates for F-NLR score groups 0, 1, and 2 were 69.1%, 42.6%, and 31.9%, respectively, there were significant differences between subgroups (P<0.01). Multivariate analysis showed that tumor size (P<0.01), pathological stage (P<0.01), and F-NLR (P<0.01) were independent prognostic factors for OS. \u0000 \u0000 \u0000Conclusion \u0000Preoperative F-NLR score can be used as an independent prognostic indicator for ESCC patients. \u0000 \u0000 \u0000Key words: \u0000Esophageal squamous cell carcinoma; Fibrinogen; Platelet to lymphocyte ratio; Prognostic indicator","PeriodicalId":10065,"journal":{"name":"中华实验外科杂志","volume":"36 1","pages":"2279-2282"},"PeriodicalIF":0.0,"publicationDate":"2019-12-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43015176","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-12-08DOI: 10.3760/CMA.J.ISSN.1001-9030.2019.12.023
Zhenlin Hou, Guihua Wang, Junbo Hu, Jing Wang, Li Sun
Objective To investigate the mechanism of microRNA (miRNA, miR)-29b regulation of tribbles pseudokinase 2 (TRIB2) in colorectal tumors. Methods The online database was used to analyze the miRNAs that bind to the 3’Untranslated Region (UTR) of TRIB2, and the highest scored miR-29b was selected for study. The eukaryotic cell transfection technique was used to interfere with the expression of miR-29b in colorectal tumor cells; Western blotting and real-time quantitative polymerase chain reaction (Real-time PCR) was used to detect the difference in protein and mRNA expression of TRIB2; the position of miR-29b binding on TRIB2-3’untranslated regions (3’UTR) was confirmed by luciferase reporter gene assay; β-galactosidase staining was used to detect effects of miR-29b on colorectal cancer cell senescence. All data were quantified as Mean±SD. Two-tailed Student’s t-test was used to evaluate the differences between two groups. All statistical analyses were performed using SPSS 24.0 (SPSS Inc.). Results In SW48 and SW480 cell lines overexpressing miR-29b, the mRNA levels of TRIB2 decreased to (63.468±4.154)% and (43.145±7.523)% (t=5.351, P 0.05). In the cancer cells overexpressing of miR-29b, the proportion of senescent cells increased from 41.473% to 62.085% (χ2=19.731, P<0.01), and overexpression of TRIB2 reduced the proportion to 46.866% (χ2=12.031, P<0.01). Conclusion miR-29b can inhibits TRIB2 expression and promote the senescence of colorectal tumors, and it can be used as a potential therapeutic target for colorectal cancer. Key words: MicroRNA-29b; Tribbles pseudokinase 2; Colorectal cancer; Cell senescence
{"title":"MicroRNA-29b promotes cellular senescence of colorectal tumors by targeting tribbles pseudokinase 2","authors":"Zhenlin Hou, Guihua Wang, Junbo Hu, Jing Wang, Li Sun","doi":"10.3760/CMA.J.ISSN.1001-9030.2019.12.023","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.1001-9030.2019.12.023","url":null,"abstract":"Objective \u0000To investigate the mechanism of microRNA (miRNA, miR)-29b regulation of tribbles pseudokinase 2 (TRIB2) in colorectal tumors. \u0000 \u0000 \u0000Methods \u0000The online database was used to analyze the miRNAs that bind to the 3’Untranslated Region (UTR) of TRIB2, and the highest scored miR-29b was selected for study. The eukaryotic cell transfection technique was used to interfere with the expression of miR-29b in colorectal tumor cells; Western blotting and real-time quantitative polymerase chain reaction (Real-time PCR) was used to detect the difference in protein and mRNA expression of TRIB2; the position of miR-29b binding on TRIB2-3’untranslated regions (3’UTR) was confirmed by luciferase reporter gene assay; β-galactosidase staining was used to detect effects of miR-29b on colorectal cancer cell senescence. All data were quantified as Mean±SD. Two-tailed Student’s t-test was used to evaluate the differences between two groups. All statistical analyses were performed using SPSS 24.0 (SPSS Inc.). \u0000 \u0000 \u0000Results \u0000In SW48 and SW480 cell lines overexpressing miR-29b, the mRNA levels of TRIB2 decreased to (63.468±4.154)% and (43.145±7.523)% (t=5.351, P 0.05). In the cancer cells overexpressing of miR-29b, the proportion of senescent cells increased from 41.473% to 62.085% (χ2=19.731, P<0.01), and overexpression of TRIB2 reduced the proportion to 46.866% (χ2=12.031, P<0.01). \u0000 \u0000 \u0000Conclusion \u0000miR-29b can inhibits TRIB2 expression and promote the senescence of colorectal tumors, and it can be used as a potential therapeutic target for colorectal cancer. \u0000 \u0000 \u0000Key words: \u0000MicroRNA-29b; Tribbles pseudokinase 2; Colorectal cancer; Cell senescence","PeriodicalId":10065,"journal":{"name":"中华实验外科杂志","volume":"36 1","pages":"2203-2206"},"PeriodicalIF":0.0,"publicationDate":"2019-12-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44893828","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Objective To study the Expression of anoctamin 7 (ANO7) in the nuclear factor kappa B (NF-κB) signal pathway and its clinical significance in prostate cancer. Methods NF-κB signal pathway activated cell and animal model were constructed using tribbles homolog1 (TRIB1) overexpressed prostate cancer cell line. NF-κB signal pathway inhibited cell model was constructed using NF-κB signal pathway inhibitor. Western blotting and real-time quantitative polymerase chain reaction (Real-time PCR) and immunohistochemistry (IHC) were used to analyze the effect of NF-κB signal pathway on the regulation of ANO7 expression and the relationship between ANO7 expression and clinicopathological features in prostate cancer. Results ANO7 expression was up-regulated in the NF-κB signal pathway-activated cell and animal models. ANO7 expression was down-regulated in the NF-κB signal pathway-inhibited cell models. The high expression level of ANO7 was 45.8% in tumor tissues compared with 85.7% in adjacent non-cancerous tissues, with statistical significance (χ2=7.258, P<0.01). The expression of ANO7 was significantly correlated with Gleason score and pathological grade (χ2=19.797, 19.797, P<0.01). Similar results were obtained from The Cancer Genome Atlas (TCGA) and the Taylor database. Kaplan-Meier analysis found that ANO7 was significantly associated with the patients’prognosis in the Taylor database (P<0.01). Multivariate analysis found that ANO7 (P<0.05), Gleason score (P<0.01) and pathological grade (P<0.01) were independent predictors of prostate cancer using the Cox regression model in the cohort affecting prostate cancer prognosis. Conclusion NF-κB signal pathway regulates ANO7 expression in prostate cancer and the expression of ANO7 is closely related to prostate cancer. Key words: Anoctamin 7; Nuclear factor kappa B signal pathway; Prostate cancer; Prognosis
{"title":"Expression of anoctamin 7 in nuclear factor kappa B signal pathway and its clinical significance in prostate cancer","authors":"Yong Luo, Guian Zhang, Xuejin Zhu, Ren Liu, Wanxiang You, Q. Qian, Xiaoming Xu, Ren-Qiang He, Weide Zhong","doi":"10.3760/CMA.J.ISSN.1001-9030.2019.12.003","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.1001-9030.2019.12.003","url":null,"abstract":"Objective \u0000To study the Expression of anoctamin 7 (ANO7) in the nuclear factor kappa B (NF-κB) signal pathway and its clinical significance in prostate cancer. \u0000 \u0000 \u0000Methods \u0000NF-κB signal pathway activated cell and animal model were constructed using tribbles homolog1 (TRIB1) overexpressed prostate cancer cell line. NF-κB signal pathway inhibited cell model was constructed using NF-κB signal pathway inhibitor. Western blotting and real-time quantitative polymerase chain reaction (Real-time PCR) and immunohistochemistry (IHC) were used to analyze the effect of NF-κB signal pathway on the regulation of ANO7 expression and the relationship between ANO7 expression and clinicopathological features in prostate cancer. \u0000 \u0000 \u0000Results \u0000ANO7 expression was up-regulated in the NF-κB signal pathway-activated cell and animal models. ANO7 expression was down-regulated in the NF-κB signal pathway-inhibited cell models. The high expression level of ANO7 was 45.8% in tumor tissues compared with 85.7% in adjacent non-cancerous tissues, with statistical significance (χ2=7.258, P<0.01). The expression of ANO7 was significantly correlated with Gleason score and pathological grade (χ2=19.797, 19.797, P<0.01). Similar results were obtained from The Cancer Genome Atlas (TCGA) and the Taylor database. Kaplan-Meier analysis found that ANO7 was significantly associated with the patients’prognosis in the Taylor database (P<0.01). Multivariate analysis found that ANO7 (P<0.05), Gleason score (P<0.01) and pathological grade (P<0.01) were independent predictors of prostate cancer using the Cox regression model in the cohort affecting prostate cancer prognosis. \u0000 \u0000 \u0000Conclusion \u0000NF-κB signal pathway regulates ANO7 expression in prostate cancer and the expression of ANO7 is closely related to prostate cancer. \u0000 \u0000 \u0000Key words: \u0000Anoctamin 7; Nuclear factor kappa B signal pathway; Prostate cancer; Prognosis","PeriodicalId":10065,"journal":{"name":"中华实验外科杂志","volume":"36 1","pages":"2137-2140"},"PeriodicalIF":0.0,"publicationDate":"2019-12-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46424174","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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