Pub Date : 2019-12-08DOI: 10.3760/CMA.J.ISSN.1001-9030.2019.12.033
Hai-bin Yu, Weihua Huang, Fangtao Zhu
Objective To investigate the effect protection of trimetazidine pretreatment on myocardial ischemia-reperfusion injury (MIRI) in rats and its mechanism. Methods Thirty health male Sprague-Dawley rats were randomly divided into three groups, Sham group (group A), ischemia-reperfusion group (group B), trimetazidine preconditioning groups (group C). Hematoxylin-eosinstaining (HE) method was used to identify the myocardial tissue. DNA in situ end labeling (TUNEL) was used to detected the apoptosis of cardiomyocytes. The serum levels of creatine kinase (CK), creatine kinase isoenzyme MB (CK-MB), superoxide dismutase (SOD), malondialdehyde (MDA) were measured and the expression of mRNA level of B cell lymphoma/leukemia-2 (bcl-2), bcl-2 associated X protein (bax) and cysteinyl aspartate-specific protease (Caspase)-3 were measured by the method of reverse transcription polymerase chain reaction (RT-PCR). Statisticalanalysis of data using the statistical product and service solutions 19.0 software. Results Compared with the group A, group B and C were present clear myocardial ischemia and myocardial infarction area. In group B, the myocardial cells were severely edematous and the fibers were disordered. In group C, the swelling myocardial cells were alleviated and the apoptosis rate of cardiomyocytes was significantly decreased (F=509.000, P<0.01). Compared with the group B, the activity of SOD (84.21±6.07) μg/L and the expression of bcl-2 at the mRNA level (3.12±1.86)in group C were increased (t=15.399, 16.141, P<0.01). The content of MDA, CK, CK-MB (33.58±3.73) mmol/L, (177.93±5.11) U/L, (50.92±2.94) U/L and The level of bax, Caspase-3 at the mRNA level in group C (2.41±0.19, 2.34±0.23) were significantly decreased (t=13.563, 24.944, 13.375, 31.696, 19.004, P<0.01). Conclusion Trimetazidine pretreatment can significantly decreases the apoptosis of myocardium induced by MIRI, could protect the myocardium of rats from ischemic reperfusion injury. Key words: Trimetazidine; Myocardial ischemia; Reperfusion injury; Apoptosis
{"title":"Effect of trimetazidine preconditioning on ischemia-reperfusion injury of rat cardiomyocyte apoptosis and expression of Mn-superoxide dismutase","authors":"Hai-bin Yu, Weihua Huang, Fangtao Zhu","doi":"10.3760/CMA.J.ISSN.1001-9030.2019.12.033","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.1001-9030.2019.12.033","url":null,"abstract":"Objective \u0000To investigate the effect protection of trimetazidine pretreatment on myocardial ischemia-reperfusion injury (MIRI) in rats and its mechanism. \u0000 \u0000 \u0000Methods \u0000Thirty health male Sprague-Dawley rats were randomly divided into three groups, Sham group (group A), ischemia-reperfusion group (group B), trimetazidine preconditioning groups (group C). Hematoxylin-eosinstaining (HE) method was used to identify the myocardial tissue. DNA in situ end labeling (TUNEL) was used to detected the apoptosis of cardiomyocytes. The serum levels of creatine kinase (CK), creatine kinase isoenzyme MB (CK-MB), superoxide dismutase (SOD), malondialdehyde (MDA) were measured and the expression of mRNA level of B cell lymphoma/leukemia-2 (bcl-2), bcl-2 associated X protein (bax) and cysteinyl aspartate-specific protease (Caspase)-3 were measured by the method of reverse transcription polymerase chain reaction (RT-PCR). Statisticalanalysis of data using the statistical product and service solutions 19.0 software. \u0000 \u0000 \u0000Results \u0000Compared with the group A, group B and C were present clear myocardial ischemia and myocardial infarction area. In group B, the myocardial cells were severely edematous and the fibers were disordered. In group C, the swelling myocardial cells were alleviated and the apoptosis rate of cardiomyocytes was significantly decreased (F=509.000, P<0.01). Compared with the group B, the activity of SOD (84.21±6.07) μg/L and the expression of bcl-2 at the mRNA level (3.12±1.86)in group C were increased (t=15.399, 16.141, P<0.01). The content of MDA, CK, CK-MB (33.58±3.73) mmol/L, (177.93±5.11) U/L, (50.92±2.94) U/L and The level of bax, Caspase-3 at the mRNA level in group C (2.41±0.19, 2.34±0.23) were significantly decreased (t=13.563, 24.944, 13.375, 31.696, 19.004, P<0.01). \u0000 \u0000 \u0000Conclusion \u0000Trimetazidine pretreatment can significantly decreases the apoptosis of myocardium induced by MIRI, could protect the myocardium of rats from ischemic reperfusion injury. \u0000 \u0000 \u0000Key words: \u0000Trimetazidine; Myocardial ischemia; Reperfusion injury; Apoptosis","PeriodicalId":10065,"journal":{"name":"中华实验外科杂志","volume":"36 1","pages":"2234-2236"},"PeriodicalIF":0.0,"publicationDate":"2019-12-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49160931","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Objective To investigate the expression and significance of ring finger protein 43 (RNF43) in human breast cancer tissue. Methods Real-time quantitative reverse transcriptase-polymerase chain reaction (RT-qPCR) and Western blotting were used to detect the mRNA and protein expression levels of RNF43 in breast cancertissues (60 cases) and normaladjacent tissues (60 cases). Results The expression of RNF43 mRNA and protein in breast cancer tissues (1.216±0.112, 0.439±0.056) was significantly higher than that in adjacent normal breast tissues (0.253±0.018, 0.107±0.015) (P 2 cm group: 1.262±0.014, 0.450±0.030), pathological type (invasive ductal carcinoma group: 1.243±0.079, 0.448±0.011; invasive lobular carcinoma group: 1.108±0.044, 0.403±0.013), human epidermalgrowth factor receptor-2 (Her-2) expression (positive group: 1.178±0.009, 0.422±0.017; negative group: 1.230±0.011, 0.445±0.010) and menstrual status (premenopausal: 1.231±0.049, 0.440±0.008; postmenopausal: 1.167±0.056, 0.436±0.011) (P>0.05). Conclusion The abnormal expression of RNF43 mRNA and protein may be Associated with the oncogenesis, progress, invasion and metastasis, and may be a important prognostic predictor of breast cancer. Key words: Ring finger protein 43; Breast cancer; Metastasis
{"title":"Expression and significance of ring finger protein 43 in breast cancer","authors":"Huacheng Zhang, Liting Zhang, Wenyi Wu, Jianqing Lin, Xinquan Wu, Yihuang Yu, Mingji Ding, Zhong-xin Huang, Jianlong Qiu","doi":"10.3760/CMA.J.ISSN.1001-9030.2019.12.043","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.1001-9030.2019.12.043","url":null,"abstract":"Objective \u0000To investigate the expression and significance of ring finger protein 43 (RNF43) in human breast cancer tissue. \u0000 \u0000 \u0000Methods \u0000Real-time quantitative reverse transcriptase-polymerase chain reaction (RT-qPCR) and Western blotting were used to detect the mRNA and protein expression levels of RNF43 in breast cancertissues (60 cases) and normaladjacent tissues (60 cases). \u0000 \u0000 \u0000Results \u0000The expression of RNF43 mRNA and protein in breast cancer tissues (1.216±0.112, 0.439±0.056) was significantly higher than that in adjacent normal breast tissues (0.253±0.018, 0.107±0.015) (P 2 cm group: 1.262±0.014, 0.450±0.030), pathological type (invasive ductal carcinoma group: 1.243±0.079, 0.448±0.011; invasive lobular carcinoma group: 1.108±0.044, 0.403±0.013), human epidermalgrowth factor receptor-2 (Her-2) expression (positive group: 1.178±0.009, 0.422±0.017; negative group: 1.230±0.011, 0.445±0.010) and menstrual status (premenopausal: 1.231±0.049, 0.440±0.008; postmenopausal: 1.167±0.056, 0.436±0.011) (P>0.05). \u0000 \u0000 \u0000Conclusion \u0000The abnormal expression of RNF43 mRNA and protein may be Associated with the oncogenesis, progress, invasion and metastasis, and may be a important prognostic predictor of breast cancer. \u0000 \u0000 \u0000Key words: \u0000Ring finger protein 43; Breast cancer; Metastasis","PeriodicalId":10065,"journal":{"name":"中华实验外科杂志","volume":"36 1","pages":"2264-2268"},"PeriodicalIF":0.0,"publicationDate":"2019-12-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45312923","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-12-08DOI: 10.3760/CMA.J.ISSN.1001-9030.2019.12.040
Gang Chen, S. Tao, Changshun Chen, Hai-Zhen Zuo
Objective Observe the effect of low-intensity pulsed ultrasound on regeneration after peripheral nerve injury. Methods Eighty rats were randomly divided into implanted and injured rat right sciatic nerve preparation model. The experimental group was treated with low-intensity pulsed ultrasound to treat the experimental group without treatment. The nerve injury of the replacement rats was treated after treatment. The post-regeneration situation is evaluated. Results Rats in the experimental group were treated with SFI and SNCV for 4 week [(29.1±5.1), (11.0±2.7) mm], 6 week [(27.5±4.5), (15.8±2.9) mm], and 8 week [(23.2±4.5), (21.3±2.7) mm]. The time was significantly different from the rats, the difference was statistically significant (t=2.630, 3.060, 3.140, 2.280, 2.650, 5.120, P<0.05); 2 week [(188.2±33.4) no/mm2], the nerve fiber density of the experimental group was slightly lower than that of the rats, implanted for 4 week [(2 385.4±394.2) no/mm2], 6 week [(2 439.4±334.2) no/mm2] and 8 week [(3 259.7±416.7) no/mm2], the nerve fiber density of the experimental group was significantly higher than the above rats, the difference was statistically significant (t=2.240, 2.430, 2.740, 3.280, P<0.05); the rats in the experimental group had a mean nerve regeneration rate of 4 week [(1.42±0.44) mm/d], 6 week [(1.48±0.44) mm/d] and 8 week [(1.29±0.28) mm/d]. Focusing on rats, the difference was statistically significant (t=2.500, 2.840, 3.070, P<0.05); the nerve fiber regeneration and Schwann cell proliferation in the experimental group were significantly replaced by rats. Conclusion Low-intensity pulsed ultrasound can effectively promote regeneration after peripheral nerve injury, and the nerve function is effectively restored, and the effect is remarkable. Key words: Low intensity pulsed ultrasound; Peripheral nerve; Damage; Regeneration; Experimental study
{"title":"Low-intensity pulsed ultrasound improvedthe regeneration ofinjuried peripheral nerve","authors":"Gang Chen, S. Tao, Changshun Chen, Hai-Zhen Zuo","doi":"10.3760/CMA.J.ISSN.1001-9030.2019.12.040","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.1001-9030.2019.12.040","url":null,"abstract":"Objective \u0000Observe the effect of low-intensity pulsed ultrasound on regeneration after peripheral nerve injury. \u0000 \u0000 \u0000Methods \u0000Eighty rats were randomly divided into implanted and injured rat right sciatic nerve preparation model. The experimental group was treated with low-intensity pulsed ultrasound to treat the experimental group without treatment. The nerve injury of the replacement rats was treated after treatment. The post-regeneration situation is evaluated. \u0000 \u0000 \u0000Results \u0000Rats in the experimental group were treated with SFI and SNCV for 4 week [(29.1±5.1), (11.0±2.7) mm], 6 week [(27.5±4.5), (15.8±2.9) mm], and 8 week [(23.2±4.5), (21.3±2.7) mm]. The time was significantly different from the rats, the difference was statistically significant (t=2.630, 3.060, 3.140, 2.280, 2.650, 5.120, P<0.05); 2 week [(188.2±33.4) no/mm2], the nerve fiber density of the experimental group was slightly lower than that of the rats, implanted for 4 week [(2 385.4±394.2) no/mm2], 6 week [(2 439.4±334.2) no/mm2] and 8 week [(3 259.7±416.7) no/mm2], the nerve fiber density of the experimental group was significantly higher than the above rats, the difference was statistically significant (t=2.240, 2.430, 2.740, 3.280, P<0.05); the rats in the experimental group had a mean nerve regeneration rate of 4 week [(1.42±0.44) mm/d], 6 week [(1.48±0.44) mm/d] and 8 week [(1.29±0.28) mm/d]. Focusing on rats, the difference was statistically significant (t=2.500, 2.840, 3.070, P<0.05); the nerve fiber regeneration and Schwann cell proliferation in the experimental group were significantly replaced by rats. \u0000 \u0000 \u0000Conclusion \u0000Low-intensity pulsed ultrasound can effectively promote regeneration after peripheral nerve injury, and the nerve function is effectively restored, and the effect is remarkable. \u0000 \u0000 \u0000Key words: \u0000Low intensity pulsed ultrasound; Peripheral nerve; Damage; Regeneration; Experimental study","PeriodicalId":10065,"journal":{"name":"中华实验外科杂志","volume":"36 1","pages":"2257-2259"},"PeriodicalIF":0.0,"publicationDate":"2019-12-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45423400","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Objective To explore the ability of reconstructing cervical vertebrae of platelet-rich plasma (PRP) and CAGE with the compound of humeral cortical ring allograft (HCA) packed with cancellous allogenic bone (CALB), and find some evidence to support the clinical practice of PRP-CAGE complex on reconstruction of vertebral body. Methods The animal models of a cervical vertebrae defect were created by surgery in 26 New Zealand white rabbits. The A group were treated with the compound of PRP-CAGE; the B group with autogenous iliac crest. Observations were made by gross, X-ray, histopathological, scanning electron microscope (SEM) examination and the effect were determined by biomechanics at different periods postoperatively.Statistical methods is t Test, there was significant difference in statistics (P<0.05). Results The gross and X-ray examination showed that spine fusion in A and B group at 2 months. Histological analysis exhibited scattered bone island and bone trabecula formation at 1 month, many mature bone matrix and bone marrow cavity formation at 2 months; the SEM also showed that there were many new bone and osteoblast formed in tow groups. The flexion values on the tests of the 50 N load-strain in flexion, extension were 52.33±3.10, 53.98±3.80 in 2 groups, The extension values were 42.63±2.80, 47.23±2.30 in 2 groups. The effect of biomechanics had no statistically in tow groups (t=0.583, 2.199, P>0.05). Conclusion The compound of PRP-Cage possesses much high bone inductive potentialities and biomechanic stability. PRP may be associated with the role of multiple growth factors in regulating cell function, improving the tissue microenvironment, and promoting intervertebral fusion. Key words: Autologous platelet-rich plasma; Transplantation, homologous; Spinal fusion; Cervical vertebrae
{"title":"Biological study of platelet-rich plasma-Cage complex on reconstruction of cervical vertebrae","authors":"Jian-peng Zhou, Yingfeng Cai, Weijun Zhou, Baoxin Liu, Hao-Dong Liang, Lixin Tian","doi":"10.3760/CMA.J.ISSN.1001-9030.2019.12.037","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.1001-9030.2019.12.037","url":null,"abstract":"Objective \u0000To explore the ability of reconstructing cervical vertebrae of platelet-rich plasma (PRP) and CAGE with the compound of humeral cortical ring allograft (HCA) packed with cancellous allogenic bone (CALB), and find some evidence to support the clinical practice of PRP-CAGE complex on reconstruction of vertebral body. \u0000 \u0000 \u0000Methods \u0000The animal models of a cervical vertebrae defect were created by surgery in 26 New Zealand white rabbits. The A group were treated with the compound of PRP-CAGE; the B group with autogenous iliac crest. Observations were made by gross, X-ray, histopathological, scanning electron microscope (SEM) examination and the effect were determined by biomechanics at different periods postoperatively.Statistical methods is t Test, there was significant difference in statistics (P<0.05). \u0000 \u0000 \u0000Results \u0000The gross and X-ray examination showed that spine fusion in A and B group at 2 months. Histological analysis exhibited scattered bone island and bone trabecula formation at 1 month, many mature bone matrix and bone marrow cavity formation at 2 months; the SEM also showed that there were many new bone and osteoblast formed in tow groups. The flexion values on the tests of the 50 N load-strain in flexion, extension were 52.33±3.10, 53.98±3.80 in 2 groups, The extension values were 42.63±2.80, 47.23±2.30 in 2 groups. The effect of biomechanics had no statistically in tow groups (t=0.583, 2.199, P>0.05). \u0000 \u0000 \u0000Conclusion \u0000The compound of PRP-Cage possesses much high bone inductive potentialities and biomechanic stability. PRP may be associated with the role of multiple growth factors in regulating cell function, improving the tissue microenvironment, and promoting intervertebral fusion. \u0000 \u0000 \u0000Key words: \u0000Autologous platelet-rich plasma; Transplantation, homologous; Spinal fusion; Cervical vertebrae","PeriodicalId":10065,"journal":{"name":"中华实验外科杂志","volume":"36 1","pages":"2247-2249"},"PeriodicalIF":0.0,"publicationDate":"2019-12-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43671134","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-12-08DOI: 10.3760/CMA.J.ISSN.1001-9030.2019.12.057
Yukun Zhang, Enrui Liu, Kangjia Luo
Colorectal cancer is one of the most common malignancies around the world that seriously affects human life and health. Most studies used cell line experiments to investigate the characteristics of colorectal cancer, but the foundation of colorectalcancer (CRC) in situ mouse model would better imitate the occurrence the process of CRC, and improve the credibility of basic experiments greatly. In the present, azoxymethane/dextran sulfate sodium (AOM/DSS)-induced cancer model is widely use in the building of CRC animal model, but there are quite differences exist in the quantities of research. As a result, this review will concisely introduce the process and assessment of the AOM/DSS-induced mouse CRC model in order to provide a comprehensive reference for the one does this ailment model. Key words: Azoxymethane/dextran sulfate sodium; Inflammation-induced cancer; Model, mouse; Assessment of model
{"title":"Azoxymethane-dextran sulfate sodium induces colorectal cacner mouse model","authors":"Yukun Zhang, Enrui Liu, Kangjia Luo","doi":"10.3760/CMA.J.ISSN.1001-9030.2019.12.057","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.1001-9030.2019.12.057","url":null,"abstract":"Colorectal cancer is one of the most common malignancies around the world that seriously affects human life and health. Most studies used cell line experiments to investigate the characteristics of colorectal cancer, but the foundation of colorectalcancer (CRC) in situ mouse model would better imitate the occurrence the process of CRC, and improve the credibility of basic experiments greatly. In the present, azoxymethane/dextran sulfate sodium (AOM/DSS)-induced cancer model is widely use in the building of CRC animal model, but there are quite differences exist in the quantities of research. As a result, this review will concisely introduce the process and assessment of the AOM/DSS-induced mouse CRC model in order to provide a comprehensive reference for the one does this ailment model. \u0000 \u0000 \u0000Key words: \u0000Azoxymethane/dextran sulfate sodium; Inflammation-induced cancer; Model, mouse; Assessment of model","PeriodicalId":10065,"journal":{"name":"中华实验外科杂志","volume":"36 1","pages":"2306-2309"},"PeriodicalIF":0.0,"publicationDate":"2019-12-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43730748","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
ObjectiveTo explore the physical properties of bone cements loaded with atituberculosis drugs.MethodsWe mixed ten drugs with four bone cements by 1.5 g∶40.0 g and 2.5 g∶40.0 g, 400 modules of 80 groups were maded according to the ISO. The control group mixed bone cements with their liquid monomer, 20 modules of 4 groups were maded. The physical properties were measured respectively.ResultsGroup 1.5 g and 2.5 g of E, Z, S, C, A, M mixed with Palacos R, Palacos MV, Simplex P, the average dough time, solidification time, maximum temperature and mechanical strength were all meet ISO, and hardened in 20 minutes after mixing. Group 1.5 g and 2.5 g of R, H, Rt, and P mixed with the bone cements above, the average dough time, solidification time were all >ISO, the average maximum temperature was 0.05) between the four kinds of bone cements. The flexural strength and flexural modulus (P<0.01) were different. The physical properties of Palacos R and Simplex P bone cements meet ISO. The flexural strength and flexural modulus of CEMEX XL bone cement were
目的探讨抗结核药物负载骨水泥的物理性能。方法将10种药物与4种骨水泥按1.5 g∶40.0 g和2.5 g∶40.0 g的比例混合，按ISO标准制作80组共400个模块。对照组将骨水泥与其液体单体混合，制作4组共20个模块。分别测定了其物理性能。结果1.5 g和2.5 g E、Z、S、C、A、M与Palacos R、Palacos MV、Simplex P混合后，平均面团时间、凝固时间、最高温度和机械强度均满足ISO要求，并在混合后20 min内硬化。1.5 g和2.5 g R、H、Rt、P与以上4种骨水泥混合后，4种骨水泥的平均成型时间、凝固时间均为>ISO，平均最高温度为0.05)。抗弯强度和抗弯模量差异有统计学意义(P<0.01)。Palacos R和Simplex P骨水泥的物理性能符合ISO标准。CEMEX XL骨水泥抗弯强度和抗弯模量均
{"title":"Screening of bone cements loaded with antituberculosis drugs: preparation and determination of physical properties","authors":"Hucheng Yuan, Mao Wenxin, Qian Wang, Zili Wang, Jiancun Lei, Q. Liang, Guangwei Sun, Xuehua Ma, Zhen Liu, Xiaoming Zhang, Jianghua Du","doi":"10.3760/CMA.J.ISSN.1001-9030.2019.12.041","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.1001-9030.2019.12.041","url":null,"abstract":"ObjectiveTo explore the physical properties of bone cements loaded with atituberculosis drugs.MethodsWe mixed ten drugs with four bone cements by 1.5 g∶40.0 g and 2.5 g∶40.0 g, 400 modules of 80 groups were maded according to the ISO. The control group mixed bone cements with their liquid monomer, 20 modules of 4 groups were maded. The physical properties were measured respectively.ResultsGroup 1.5 g and 2.5 g of E, Z, S, C, A, M mixed with Palacos R, Palacos MV, Simplex P, the average dough time, solidification time, maximum temperature and mechanical strength were all meet ISO, and hardened in 20 minutes after mixing. Group 1.5 g and 2.5 g of R, H, Rt, and P mixed with the bone cements above, the average dough time, solidification time were all >ISO, the average maximum temperature was 0.05) between the four kinds of bone cements. The flexural strength and flexural modulus (P<0.01) were different. The physical properties of Palacos R and Simplex P bone cements meet ISO. The flexural strength and flexural modulus of CEMEX XL bone cement were<ISO, and Palacos MV bone cement flexural modulus <ISO.Conclusion(1) Antituberculosis drugs Z, E, S, C, M, and A were suitable for preparing bone cements. (2) Antituberculosis drugs R, H, Rt, and P were not suitable for preparing bone cements. (3) CEMEX XL were not suitable for drug loading.Key words: Antituberculosis drugs; Bone cement; Screening; Physical property","PeriodicalId":10065,"journal":{"name":"中华实验外科杂志","volume":"36 1","pages":"2260-2263"},"PeriodicalIF":0.0,"publicationDate":"2019-12-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48142601","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-12-08DOI: 10.3760/CMA.J.ISSN.1001-9030.2019.12.058
Pengcheng Xiang, Jie Tang, Peng-ju Li, Junyi Han
Immunotherapy for malignant tumors has been widely recognized as an important means of tumor therapy, but so far most of the studies have focused on specific immunity. In recent years, with the research progress of innate immunity, the relationship between innate lymphoid cells and tumor has been paid more and more attention. At present, there are few related studies, and the direct research evidence about the relationship between innate lymphoid cells and tumor is not very abundant. In this paper, they are briefly divided into five categories according to their origin, function and other characteristics. Considering the relative abundance of natural killer (NK) cell, we focus on the relationship between NK cell and its cytokines and tumor. Key words: Innate lymphoid cells; Natural killer cell; Tumor; Tumor Immunity
{"title":"The relationship between innate lymphoid cell and tumor","authors":"Pengcheng Xiang, Jie Tang, Peng-ju Li, Junyi Han","doi":"10.3760/CMA.J.ISSN.1001-9030.2019.12.058","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.1001-9030.2019.12.058","url":null,"abstract":"Immunotherapy for malignant tumors has been widely recognized as an important means of tumor therapy, but so far most of the studies have focused on specific immunity. In recent years, with the research progress of innate immunity, the relationship between innate lymphoid cells and tumor has been paid more and more attention. At present, there are few related studies, and the direct research evidence about the relationship between innate lymphoid cells and tumor is not very abundant. In this paper, they are briefly divided into five categories according to their origin, function and other characteristics. Considering the relative abundance of natural killer (NK) cell, we focus on the relationship between NK cell and its cytokines and tumor. \u0000 \u0000 \u0000Key words: \u0000Innate lymphoid cells; Natural killer cell; Tumor; Tumor Immunity","PeriodicalId":10065,"journal":{"name":"中华实验外科杂志","volume":"36 1","pages":"2309-2312"},"PeriodicalIF":0.0,"publicationDate":"2019-12-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42148189","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Objective We investigate the molecular mechanism underlying inhibitory effects of cordycepin on proliferation, apoptosis, migration and invasion of breast cancer. We focus on the role of HOXD10 in inhibitory effects of cordycepin. Methods Two individual breast cancer cell lines, MCF-7 and MDA-MB-231, were used in this study to investigate the effects of cordycepin on proliferation, apoptosis, migration and invasion of breast cancer, by cell counting kit-8 (CCK-8) assays, flow cytometry and Transwell assays. The small interfering RNAs (siRNAs) targeted HOXD10 were transfected into MCF-7 and MDA-MB-231 cells to knock down HOXD10. We investigate the role of HOXD10 by comparing the difference between group NC and group siRNAs. Results The A values of cordycepin treated MCF-7 and MDA-MB-231 cells were significantly lower than those of control group (DMSO group) (MCF-7cells: 0.665±0.004 vs. 0.733±0.005, t=10.450, and MDA-MB-231cells: 0.632±0.005 vs. 0.722±0.005, t=13.330, P<0.05), which means the proliferation of breast cancer cells was significantly inhibited, The apoptosis rateof cordycepin treated MCF-7 and MDA-MB-231 cells were significantly higher than those of control group (MCF-7cells: 20.200±0.322 vs. 5.500±0.000, t=45.730, MDA-MB-231cells: 21.800±1.493 vs. 5.367±0.318, t=10.760, P<0.05). There were significantly fewer migrated MCF-7 and MDA-MB-231 cells in cordycepin group than in control group(MCF-7 cells: 28.670±1.764 vs. 83.330±2.186, t=19.460, MDA-MB-231cells: 29.000±2.646 vs. 114.700±3.180, t=20.710, P<0.05). There were significantly fewer invasive MCF-7 and MDA-MB-231 cells in cordycepin group than control group(MCF-7cells: 24.670±2.603 vs. 49.000±1.528, t=8.0620, MDA-MB-231cells: 12.330±1.453 vs. 36.670±2.728, t=7.872, P<0.05). After transfection of MCF-7 and MDA-MB-231 cells with siRNA and intervention with cordycepin, the proliferation of breast cancer cells was inhibited (MCF-7cells: 0.627±0.004 vs. 0.648±0.006, t=2.951, MDA-MB-23 cells: 0.620±0.006 vs. 0.635±0.004, t=2.087, P<0.05). The apoptosis rate of the treatment group was significantly higher than the control group (MCF-7 cells: 20.470±0.260 vs. 16.300±0.153, t=13.800, MDA-MB-23 cells: 19.170±0.167 vs. 17.030±0.186, t=8.5520, P<0.05). There were significantly fewer migrated MCF-7 and MDA-MB-231 cells in siRNA group than in control group(MCF-7cells: 11.000±2.082 vs. 30.330±2.028, t=6.653, MDA-MB-23cells: 11.330±1.4530 vs. 23.000±1.528, t=5.534, P<0.05). There were significantly fewer invasive MCF-7 and MDA-MB-231cells in siRNA group than control group(MCF-7 cells: 16.330±1.764 vs. 23.670±1.760, t=2.940, MDA-MB-2 cells: 9.333±1.453 vs. 19.670±2.333, t=3.759, P<0.05). Those values above are statistically significant. Conclusion Cordycepin induces apoptosis and inhibits proliferation, migration and invasion of breast cancer.2. Suppression of HOXD10 promptes the effects of cordycepin on proliferation, apoptosis, migration and invasion of breast cancer. Key w
{"title":"Effects of HOXD10 on the mechanism of cordycepin action in different types of human breast cancer cells","authors":"Yuan-qi Zhang, Liyan Yu, Zhidan Chen, Sheng-chao Huang, Zeming Yan, X. Sui, Zhongzeng Liang, Baoyi Huang, Kangwei Luo, Mia Yu, Hai-Xia Huang, Xiao-dong Chen","doi":"10.3760/CMA.J.ISSN.1001-9030.2019.12.013","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.1001-9030.2019.12.013","url":null,"abstract":"Objective \u0000We investigate the molecular mechanism underlying inhibitory effects of cordycepin on proliferation, apoptosis, migration and invasion of breast cancer. We focus on the role of HOXD10 in inhibitory effects of cordycepin. \u0000 \u0000 \u0000Methods \u0000Two individual breast cancer cell lines, MCF-7 and MDA-MB-231, were used in this study to investigate the effects of cordycepin on proliferation, apoptosis, migration and invasion of breast cancer, by cell counting kit-8 (CCK-8) assays, flow cytometry and Transwell assays. The small interfering RNAs (siRNAs) targeted HOXD10 were transfected into MCF-7 and MDA-MB-231 cells to knock down HOXD10. We investigate the role of HOXD10 by comparing the difference between group NC and group siRNAs. \u0000 \u0000 \u0000Results \u0000The A values of cordycepin treated MCF-7 and MDA-MB-231 cells were significantly lower than those of control group (DMSO group) (MCF-7cells: 0.665±0.004 vs. 0.733±0.005, t=10.450, and MDA-MB-231cells: 0.632±0.005 vs. 0.722±0.005, t=13.330, P<0.05), which means the proliferation of breast cancer cells was significantly inhibited, The apoptosis rateof cordycepin treated MCF-7 and MDA-MB-231 cells were significantly higher than those of control group (MCF-7cells: 20.200±0.322 vs. 5.500±0.000, t=45.730, MDA-MB-231cells: 21.800±1.493 vs. 5.367±0.318, t=10.760, P<0.05). There were significantly fewer migrated MCF-7 and MDA-MB-231 cells in cordycepin group than in control group(MCF-7 cells: 28.670±1.764 vs. 83.330±2.186, t=19.460, MDA-MB-231cells: 29.000±2.646 vs. 114.700±3.180, t=20.710, P<0.05). There were significantly fewer invasive MCF-7 and MDA-MB-231 cells in cordycepin group than control group(MCF-7cells: 24.670±2.603 vs. 49.000±1.528, t=8.0620, MDA-MB-231cells: 12.330±1.453 vs. 36.670±2.728, t=7.872, P<0.05). After transfection of MCF-7 and MDA-MB-231 cells with siRNA and intervention with cordycepin, the proliferation of breast cancer cells was inhibited (MCF-7cells: 0.627±0.004 vs. 0.648±0.006, t=2.951, MDA-MB-23 cells: 0.620±0.006 vs. 0.635±0.004, t=2.087, P<0.05). The apoptosis rate of the treatment group was significantly higher than the control group (MCF-7 cells: 20.470±0.260 vs. 16.300±0.153, t=13.800, MDA-MB-23 cells: 19.170±0.167 vs. 17.030±0.186, t=8.5520, P<0.05). There were significantly fewer migrated MCF-7 and MDA-MB-231 cells in siRNA group than in control group(MCF-7cells: 11.000±2.082 vs. 30.330±2.028, t=6.653, MDA-MB-23cells: 11.330±1.4530 vs. 23.000±1.528, t=5.534, P<0.05). There were significantly fewer invasive MCF-7 and MDA-MB-231cells in siRNA group than control group(MCF-7 cells: 16.330±1.764 vs. 23.670±1.760, t=2.940, MDA-MB-2 cells: 9.333±1.453 vs. 19.670±2.333, t=3.759, P<0.05). Those values above are statistically significant. \u0000 \u0000 \u0000Conclusion \u0000Cordycepin induces apoptosis and inhibits proliferation, migration and invasion of breast cancer.2. Suppression of HOXD10 promptes the effects of cordycepin on proliferation, apoptosis, migration and invasion of breast cancer. \u0000 \u0000 \u0000Key w","PeriodicalId":10065,"journal":{"name":"中华实验外科杂志","volume":"36 1","pages":"2170-2172"},"PeriodicalIF":0.0,"publicationDate":"2019-12-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47975396","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-12-08DOI: 10.3760/CMA.J.ISSN.1001-9030.2019.12.060
Xingjia Mao
Osteoarthritis (OA) is a common and disabling joint disorder, which is mainly characterized by cartilage degeneration and narrow joint space. Nowadays, the research on the therapy of OA is no longer limited to articular cartilage. Subchondral bone plays an important role in the genesis and development of OA, both in structure and biological function. This article briefly reviews the structural and functional association of subchondral bone and cartilage and the pathological change of subchondral bone in OA. The progress in the treatment of osteoarthritis based on subchondral bone was emphatically reviewed, and a global visualized analysis of subchondral bone in recent twenty five years was shared. Key words: Subchondral bone; Osteoarthritis; Therapy; Visualized analysis
{"title":"Progress in the treatment of osteoarthritis based on subchondral bone","authors":"Xingjia Mao","doi":"10.3760/CMA.J.ISSN.1001-9030.2019.12.060","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.1001-9030.2019.12.060","url":null,"abstract":"Osteoarthritis (OA) is a common and disabling joint disorder, which is mainly characterized by cartilage degeneration and narrow joint space. Nowadays, the research on the therapy of OA is no longer limited to articular cartilage. Subchondral bone plays an important role in the genesis and development of OA, both in structure and biological function. This article briefly reviews the structural and functional association of subchondral bone and cartilage and the pathological change of subchondral bone in OA. The progress in the treatment of osteoarthritis based on subchondral bone was emphatically reviewed, and a global visualized analysis of subchondral bone in recent twenty five years was shared. \u0000 \u0000 \u0000Key words: \u0000Subchondral bone; Osteoarthritis; Therapy; Visualized analysis","PeriodicalId":10065,"journal":{"name":"中华实验外科杂志","volume":"36 1","pages":"2317-2322"},"PeriodicalIF":0.0,"publicationDate":"2019-12-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44872031","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-12-08DOI: 10.3760/CMA.J.ISSN.1001-9030.2019.12.011
Xiaofeng Xie, Li Yang, Jianheng Chen, Haiyuan Li, Yin Chen
Objective To investigate the effects of methamphetamine on gonadotropin and testosterone receptors in male rats. Methods Acute and chronic exposure models were established by intraperitoneal injection of Methamphetamine (METH). Enzyme linked immunosorbent assay (ELISA) was performed to detect the expression of testosterone (T), estradiol (E2), Follicle stimulating hormone (FSH), luteinizing hormone (LH), progesterone (PROG), prolactin (PRL), structural changes of testicular were observed by hematoxylin and eosin (HE) staining, immunohistochemical staining (IHC) and Western blotting were performed to detect the localization and expression of androgen receptor (AR), estrogen receptor alpha (ER) estrogen receptor β (ERβ) in the testis. Results ELISA results showed that compared with control group, the concentration of T in the acute exposure group was (2.16±0.51) μg/L, which was significantly decreased (t=-2.432, P<0.05). The concentrations of T, E2, PRL and INH B in the chronic exposure group were (0.41±0.37) μg/L, (73.84±33.63) ng/L, (5.86±4.27) μg/L, and (22.52±7.19) ng/L respectively. The T, E2 and INH B in the chronic exposed group was significantly decreased compared to the control group (t=-6.211, -2.853, 2.553, P<0.05). While the PRL was significantly increased (t=2.318, P<0.05). HE staining showed that the spermatogenic cells in the METH exposure group were slightly reduced and disordered. Compared with control group, IHC and Western blotting showed that the protein expression of AR, ERxpond ERβ decreased in the testis. Conclusion The acute and chronic exposure of METH in male rats could lead to gonadotropin disorder and decreased expression of testosterone receptors AR, ERed with the chronic controt the occurrence and development of the sperm. Key words: Methamphetamine; Male reproduction; Gonadal hormone; Hormone receptor
{"title":"The effects of methamphetamine on gonadotropin and androgen, estrogen receptors in male rats","authors":"Xiaofeng Xie, Li Yang, Jianheng Chen, Haiyuan Li, Yin Chen","doi":"10.3760/CMA.J.ISSN.1001-9030.2019.12.011","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.1001-9030.2019.12.011","url":null,"abstract":"Objective \u0000To investigate the effects of methamphetamine on gonadotropin and testosterone receptors in male rats. \u0000 \u0000 \u0000Methods \u0000Acute and chronic exposure models were established by intraperitoneal injection of Methamphetamine (METH). Enzyme linked immunosorbent assay (ELISA) was performed to detect the expression of testosterone (T), estradiol (E2), Follicle stimulating hormone (FSH), luteinizing hormone (LH), progesterone (PROG), prolactin (PRL), structural changes of testicular were observed by hematoxylin and eosin (HE) staining, immunohistochemical staining (IHC) and Western blotting were performed to detect the localization and expression of androgen receptor (AR), estrogen receptor alpha (ER) estrogen receptor β (ERβ) in the testis. \u0000 \u0000 \u0000Results \u0000ELISA results showed that compared with control group, the concentration of T in the acute exposure group was (2.16±0.51) μg/L, which was significantly decreased (t=-2.432, P<0.05). The concentrations of T, E2, PRL and INH B in the chronic exposure group were (0.41±0.37) μg/L, (73.84±33.63) ng/L, (5.86±4.27) μg/L, and (22.52±7.19) ng/L respectively. The T, E2 and INH B in the chronic exposed group was significantly decreased compared to the control group (t=-6.211, -2.853, 2.553, P<0.05). While the PRL was significantly increased (t=2.318, P<0.05). HE staining showed that the spermatogenic cells in the METH exposure group were slightly reduced and disordered. Compared with control group, IHC and Western blotting showed that the protein expression of AR, ERxpond ERβ decreased in the testis. \u0000 \u0000 \u0000Conclusion \u0000The acute and chronic exposure of METH in male rats could lead to gonadotropin disorder and decreased expression of testosterone receptors AR, ERed with the chronic controt the occurrence and development of the sperm. \u0000 \u0000 \u0000Key words: \u0000Methamphetamine; Male reproduction; Gonadal hormone; Hormone receptor","PeriodicalId":10065,"journal":{"name":"中华实验外科杂志","volume":"36 1","pages":"2163-2166"},"PeriodicalIF":0.0,"publicationDate":"2019-12-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43112719","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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