Journal of Pharmaceutical and Biomedical Analysis Open最新文献_第5页

How does HSA's antioxidant activity change under the influence of lincomycin and spectinomycin mixture? – A spectroscopic study 林可霉素与大观霉素混合作用对HSA的抗氧化活性有何影响？-光谱研究

Journal of Pharmaceutical and Biomedical Analysis Open

Pub Date : 2025-06-01 Epub Date: 2025-03-08 DOI: 10.1016/j.jpbao.2025.100070

Wojciech Rogóż, Aleksandra Owczarzy, Karolina Kulig, Małgorzata Maciążek-Jurczyk

Lincomycin (LIN) belongs to the lincosamides group and is used in bacterial infections of soft tissues, the respiratory system, bone marrow, and bones, while spectinomycin (SPE) belongs to the group of aminoglycoside antibiotics and is used for the treatment of gonorrhoea. Both antibiotics are commonly used to treat humans separately while their mixture is used in veterinary preparations (SPE-LIN), and the combination of these antibiotics has not yet been used for human treatment. The aim of this study was to search for modulators of human serum albumin (HSA) antioxidant activity among antibacterial drugs, lincomycin (LIN) and spectinomycin (SPE). In order to study the effect of LIN and SPE on HSA antioxidant properties the DPPH and ABTS tests were used. UV-Vis spectrophotometry and circular dichroism (CD) spectroscopic techniques were used to find the cause of LIN and SPE modulatory activity related to HSA antiradical potential. Both LIN and SPE, as well as their mixture, did not show significant antioxidant activity, while in combination with HSA, they stimulated its antioxidant properties. CD measurements determined that the dominant secondary structure of HSA, regardless of the presence of ligands, was α-helix, and its percentage share slightly increased under the influence of LIN and SPE. The obtained results confirm the viability of further studies on the potential application of the combination of SPE-LIN in the treatment, not only in breeding animals. The proposed concept of research may be a response to the future needs in medicine. The assumed goal, which was the search for HSA modulators, has been achieved.

林可霉素（Lincomycin， LIN）属于林可胺类药物，用于软组织、呼吸系统、骨髓和骨骼的细菌感染，而大观霉素（spectinomycin， SPE）属于氨基糖苷类抗生素，用于治疗淋病。这两种抗生素通常用于单独治疗人类，而它们的混合物用于兽医制剂（SPE-LIN），这些抗生素的组合尚未用于人类治疗。本研究的目的是在抗菌药物林可霉素（LIN）和大观霉素（SPE）中寻找人血清白蛋白（HSA）抗氧化活性的调节剂。为了研究LIN和SPE对HSA抗氧化性能的影响，采用DPPH和ABTS试验。利用紫外-可见分光光度法和圆二色（CD）光谱技术研究了LIN和SPE调节HSA抗自由基电位的原因。LIN和SPE及其混合物均不表现出显著的抗氧化活性，而与HSA结合后，则能激发其抗氧化性能。CD测定表明，无论配体是否存在，HSA的优势二级结构都是α-螺旋结构，在LIN和SPE的影响下，其百分比略有增加。所获得的结果证实了进一步研究SPE-LIN联合应用于治疗的可行性，而不仅仅是在育种动物中。提出的研究概念可能是对未来医学需求的回应。假设的目标，即寻找HSA调制器，已经实现。

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引用次数: 0

Determination of rapid and sensitive extraction of colchicine from Colchicum baytopiorum and Colchicum decaisnei leaves by HPLC-UV method 高效液相色谱-紫外分光光度法测定秋水仙叶和秋水仙叶中秋水仙碱的快速灵敏提取

Journal of Pharmaceutical and Biomedical Analysis Open

Pub Date : 2025-06-01 Epub Date: 2025-04-22 DOI: 10.1016/j.jpbao.2025.100076

Efe Deniz , Ümit Babacan , Ceren Selim , Mehmet Fatih Cengiz

This study focuses on the extraction and analysis of colchicine, a bioactive alkaloid, from the leaves of two endemic species, Colchicum baytopiorum (n = 13) and Colchicum decaisnei (n = 11), collected from Antalya, Türkiye. Colchicine, known for its pharmacological applications for the treatment of gout and cancer, was extracted using ultrasonic-assisted extraction, a more environmentally friendly alternative to conventional methods like Soxhlet and solid-liquid extraction. High-performance liquid chromatography was used for the quantification of colchicine levels in the plant samples. The method was validated in terms of linear dynamic range, limit of detection (LOD), limit of quantification (LOQ) and relative standard deviation (RSD). Results showed that LOD, LOQ and RSD values were determined to be 0.210 ppm, 0.799 ppm and 1.420, respectively. C. baytopiorum had a higher colchicine content (206.24 ± 87.48 ppm) compared to C. decaisnei (11.23 ± 23.04 ppm). These findings are consistent with previous studies, which report varying colchicine concentrations across different Colchicum species. The study suggests that genetic research could help identify the specific genes responsible for colchicine production, which could enhance its availability in pharmaceutical applications. This research contributes to understanding colchicine extraction and its potential for medical use.

本研究对采自土耳其安塔利亚的两种特有植物秋水仙碱（Colchicum baytopiorum, n = 13）和秋水仙（Colchicum decaisnei, n = 11）的叶片进行了生物活性成分的提取和分析。秋水仙碱以其治疗痛风和癌症的药理应用而闻名，它是用超声波辅助提取的，这是一种比索氏提取和固液提取等传统方法更环保的选择。采用高效液相色谱法定量测定植物样品中秋水仙碱的含量。从线性动态范围、检出限（LOD）、定量限（LOQ）和相对标准偏差（RSD）等方面对该方法进行了验证。结果表明，测定的LOD、LOQ和RSD分别为0.210 ppm、0.799 ppm和1.420 ppm。秋水仙碱含量（206.24 ± 87.48 ppm）高于秋水仙碱含量（11.23 ± 23.04 ppm）。这些发现与先前的研究一致，这些研究报告了不同秋水仙属植物的秋水仙碱浓度不同。该研究表明，基因研究可以帮助确定负责秋水仙碱生产的特定基因，从而可以提高其在制药应用中的可用性。本研究有助于了解秋水仙碱的提取及其潜在的医学用途。

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引用次数: 0

Progress and future directions in determining drugs using spectroelectrochemical methods 光谱电化学测定药物的研究进展及未来发展方向

Journal of Pharmaceutical and Biomedical Analysis Open

Pub Date : 2025-06-01 Epub Date: 2025-02-18 DOI: 10.1016/j.jpbao.2025.100067

Vildan Sanko , Ozge Surucu , Filiz Kuralay

The use of drugs is essential for the continuity of human health and the prevention of diseases for living creatures. A key factor in effective drug use is taking it at the correct dosage and timing. Otherwise, some adverse or side effects of these drugs may occur. For this reason, it is crucial to determine the drugs used. In some cases, drug analysis is also essential to understand the use of harmful active substances, such as in the class of prohibited ones. The simple definition of a drug (medication) is a chemical substance that produces a biological effect when adapted to an organism. There are many techniques to determine the drug amount, including spectrometric, chromatographic, electrochemical, colorimetric, or immunological. These analytical techniques have been successfully used to quantify drugs. To improve the effectiveness of the detection protocols, combined systems such as spectroelectrochemistry (SEC) provide significant advantages. This well-known technique unites the principles of spectroscopy and electrochemistry by creating a synergy to provide information on redox properties, mechanisms, and molecular structures. This review highlights the transformative potential of SEC in determining drug molecules, offering a detailed exploration of its principles, methodologies, and unique advantages. The main emphasis, however, is on the use of SEC in pharmaceutical analysis, where its accuracy and adaptability have driven significant progress. Recent studies are presented in detail, demonstrating SEC's effectiveness in detecting, quantifying, and characterizing drug molecules with high sensitivity and specificity.

药物的使用对于维持人类健康和预防生物疾病是必不可少的。有效用药的一个关键因素是在正确的剂量和时间服用药物。否则，这些药物可能会产生一些不良或副作用。因此，确定使用的药物是至关重要的。在某些情况下，药物分析对于了解有害活性物质的使用也是必不可少的，例如在违禁物质的类别中。药物（药物）的简单定义是一种化学物质，当适应于生物体时产生生物效应。测定药物量的方法有很多，包括光谱法、色谱法、电化学法、比色法或免疫学法。这些分析技术已成功地用于定量药物。为了提高检测方案的有效性，光谱电化学（SEC）等组合系统提供了显着的优势。这种众所周知的技术通过创造一种协同作用，将光谱学和电化学原理结合起来，提供有关氧化还原性质、机制和分子结构的信息。这篇综述强调了SEC在确定药物分子方面的变革潜力，提供了其原理、方法和独特优势的详细探索。然而，主要的重点是在药物分析中使用SEC，其准确性和适应性已经推动了重大进展。最近的研究详细介绍了SEC在检测、定量和表征药物分子方面的有效性，具有高灵敏度和特异性。

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引用次数: 0

Highly selective and sensitive detection of atorvastatin from using a MIP-based electrochemical sensor 基于mip的电化学传感器对阿托伐他汀的高选择性和高灵敏度检测

Journal of Pharmaceutical and Biomedical Analysis Open

Pub Date : 2025-06-01 Epub Date: 2025-02-03 DOI: 10.1016/j.jpbao.2025.100061

Ensar Piskin , Fatih Ahmet Korkut , Ahmet Cetinkaya , Sibel A. Ozkan

Atorvastatin (ATR) is one of the statins that are widely used all around the world. This study presents a molecularly imprinted polymer (MIP) based electrochemical sensor for the sensitive and selective detection of ATR in binary mixtures and commercial serum samples. The designed sensor was fabricated using a photopolymerization (PP) approach on a glassy carbon electrode (GCE) using ATR as the template and acrylamide (ACR) as the functional monomer. The developed ATR/ACR@MIP-GCE sensor was evaluated electrochemically and morphologically using electrochemical impedance spectroscopy (EIS), cyclic voltammetry (CV), and scanning electron microscopy (SEM). The indirect measuring method was used with a 5.0 mM [Fe(CN)₆]^3–/4– solution to determine the ATR in the linear range of 1.0–10.0 pM. Moreover, the sensor demonstrated excellent linearity and extremely sensitive quantification (LOQ) limit of detection (LOD) values in both biological media and standard solutions. It also performed well in terms of specificity by effectively differentiating ATR from compounds with similar structures. Excellent repeatability and reproducibility results have confirmed the reliability of the sensor. The selectivity of the ATR/ACR@MIP-GCE sensor for ATR was proven by interference studies on ezetimibe and their binary mixtures.

阿托伐他汀（ATR）是世界上广泛使用的他汀类药物之一。本研究提出了一种基于分子印迹聚合物（MIP）的电化学传感器，用于灵敏和选择性地检测二元混合物和商业血清样品中的ATR。设计的传感器以ATR为模板，丙烯酰胺（ACR）为功能单体，采用光聚合（PP）方法在玻璃碳电极（GCE）上制备。利用电化学阻抗谱（EIS）、循环伏安法（CV）和扫描电镜（SEM）对研制的ATR/ACR@MIP-GCE传感器进行了电化学和形态学评价。间接测量法采用5.0 mM [Fe(CN)6]3 - /4 -溶液，在1.0 ~ 10.0 pM线性范围内测定ATR。此外，该传感器在生物培养基和标准溶液中均表现出良好的线性和极灵敏的定量（LOQ）检测限（LOD）值。它还表现出良好的特异性，可以有效地将ATR与具有相似结构的化合物区分开来。优异的重复性和再现性结果证实了该传感器的可靠性。通过对依折替米贝及其二元混合物的干扰研究，证明了ATR/ACR@MIP-GCE传感器对ATR的选择性。

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引用次数: 0

Chitosan and carbon nanotube modified DNA biosensor for determination of drug-DNA interaction 壳聚糖和碳纳米管修饰DNA生物传感器用于药物-DNA相互作用的测定

Journal of Pharmaceutical and Biomedical Analysis Open

Pub Date : 2025-06-01 Epub Date: 2025-01-03 DOI: 10.1016/j.jpbao.2025.100050

Selen Soyalp , Ece Eksin , Arzum Erdem

In the present study Chitosan (CHIT) and Multiwalled carbon nanotube (MWCNT) modified pencil graphite electrodes (MWCNT-CHIT-PGEs) were developed for enhanced electrochemical monitoring of nucleic acid, and the interaction of the anticancer drug Mitomycin C (MC) and calf thymus double stranded DNA (ctdsDNA). The microscopic characterization of PGE and MWCNT-CHIT-PGE surfaces was carried out by scanning electron microscopy (SEM). Cyclic Voltammetry (CV) and Electrochemical Impedance Spectroscopy (EIS) techniques were used to evaluate the electrochemical performance of the MWCNT-CHIT-PGEs. The interaction between MC and ctdsDNA was investigated by measuring the oxidation signals of both MC and guanine in the same voltammetric scale using Differential pulse voltammetry (DPV) technique. The experimental parameters, dsDNA and MC concentration and the interaction time were optimized to enhance the overall efficiency of the experimental process.

本文研究了壳聚糖（CHIT）和多壁碳纳米管（MWCNT）修饰铅笔石墨电极（MWCNT-CHIT- pges）用于增强核酸的电化学监测，以及抗癌药物丝裂霉素C （MC）与小牛胸腺双链DNA （ctdsDNA）的相互作用。采用扫描电镜（SEM）对PGE和MWCNT-CHIT-PGE表面进行了微观表征。利用循环伏安法（CV）和电化学阻抗谱（EIS）技术对MWCNT-CHIT-PGEs的电化学性能进行了评价。采用差分脉冲伏安法（DPV）对MC和鸟嘌呤在同一伏安尺度下的氧化信号进行测定，研究了MC与ctdsDNA的相互作用。优化了实验参数、dsDNA和MC浓度以及相互作用时间，提高了实验过程的整体效率。

引用次数: 0

IrO2 nanoparticles-doped bacterial cellulose as a paper-based platform for optical detection of dopamine IrO2纳米颗粒掺杂细菌纤维素作为多巴胺光学检测的纸质平台

Journal of Pharmaceutical and Biomedical Analysis Open

Pub Date : 2025-06-01 Epub Date: 2025-02-19 DOI: 10.1016/j.jpbao.2025.100065

Handenur Tomaşoğlu , Eda Gumus , Erhan Zor , Haluk Bingol

Paper-based sensors including different indicators are attractive to many scientists because they can be used in many different fields. Due to their visual interpretability and ease of use, a large number of paper-based sensors have been created as competitors to traditional detection methods. In particular, the application of nanomaterials with different strategies to paper-based biosensors can provide an enhanced colorimetric readout for selective and sensitive detection. Among them, the numerous hydroxyl groups on IrO₂ nanoparticles (IrO₂NPs) surface made it easier for organic molecules to be adsorbed, leading to optical change of IrO₂NPs in the presence of biologically important molecules as analyte. In this study, a paper-based optical sensor platform was obtained using bacterial cellulose (BC) doped with IrO₂NPs. The IrO₂NPs-doped BC (IrO₂NPs@BC) was cut into circular pieces (spot) for easily handling and used for dopamine detection. The dopamine sensor showed linear response in a concentration range of 0.30 µM to 10 µM in solution, while it revealed a linear range of 0.60 mM to 2.5 mM in paper-based platform. Limit of detection (LOD) and limit of quantification (LOQ) values were determined to be 0.107 µM and 0.325 µM in solution media, and to be 0.479 mM and 1.45 mM for the proposed paper-based optical sensor platform, respectively.

包括不同指标的纸质传感器因其可用于许多不同领域而受到许多科学家的青睐。由于其视觉可解释性和易用性，大量基于纸张的传感器已经被创造出来，作为传统检测方法的竞争对手。特别是，将不同策略的纳米材料应用于基于纸张的生物传感器可以为选择性和灵敏度检测提供增强的比色读数。其中，IrO2纳米颗粒（IrO2NPs）表面大量的羟基使得有机分子更容易被吸附，导致IrO2NPs在有重要生物学分子作为分析物存在时发生光学变化。在本研究中，利用细菌纤维素（BC）掺杂IrO2NPs获得了基于纸张的光学传感器平台。将iro2nps掺杂的BC （IrO2NPs@BC）切成圆形块（点），便于处理，用于多巴胺检测。多巴胺传感器在溶液浓度为0.30 µM至10 µM范围内呈线性响应，在纸质平台上呈0.60 µM至2.5 µM线性响应。溶液介质的检出限（LOD）和定量限（LOQ）分别为0.107 µM和0.325 µM，纸质光学传感器平台的检出限（LOD）和定量限（LOQ）分别为0.479 mM和1.45 mM。

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引用次数: 0

Direct sample introduction in high-performance liquid chromatography analysis of oily medicinal cannabis samples using surfactant-free microemulsion 使用无表面活性剂微乳液对含油药用大麻样品进行高效液相色谱分析，直接进样

Journal of Pharmaceutical and Biomedical Analysis Open

Pub Date : 2025-06-01 Epub Date: 2025-03-11 DOI: 10.1016/j.jpbao.2025.100075

Ana Paula Lamounier, Bárbara da Conceição Coelho Cotta Cardoso, Alessandra Licursi Maia Cerqueira da Cunha

The therapeutic use of medicinal Cannabis has raised challenges in quality control and cannabinoid quantification in oil extracts, primarily due to incompatibility with liquid chromatography systems. Surfactant-free microemulsion (SFME) systems offer a promising, efficient, and sustainable solution, enabling direct analysis by high-performance liquid chromatography. These microemulsions are dispersions of two immiscible liquids, typically oil and water, made compatible by an amphiphilic substance that interacts with both polar and nonpolar fractions. After SFME testing and critical analysis of the results, clear and homogeneous microemulsions were observed regardless of the oil-to-water ratio studied, provided that sufficient quantities of 1-octanol:1-propanol (3:10 v/v) were added. Based on the established robust working range, the 1:2 (oil:ultrapure water) ratio (% w/w) was chosen as the compromise condition, minimizing medicinal oil use while ensuring compatibility with the chromatographic system. Univariate optimization of chromatographic conditions enabled simultaneous analysis of five cannabinoids, including cannabidiol (CBD), cannabidiolic acid (CBDA), tetrahydrocannabutol (THCB), cannabinol (CBN), and tetrahydrocannabinol (Δ9-THC) using a ZORBAX Eclipse Plus C18 column at 35 °C. The method employed isocratic elution with a mobile phase of 82:18 %v/v methanol:ultrapure water containing 0.15 ± 0.05 % v/v formic acid, a 1.0 mL min⁻¹ flow rate, and a 20 μL injection volume, using the fluorescence and UV absorption detectors. This proposal offers sensitivity (LOD in the ng mL⁻¹), selectivity, and separation efficiency, associated with low cost, minimal waste generation, and low energy demand, aligning with Green Chemistry principles.

药用大麻的治疗用途在油提取物的质量控制和大麻素定量方面提出了挑战，主要是由于与液相色谱系统不相容。无表面活性剂微乳液（SFME）系统提供了一种有前途的、高效的、可持续的解决方案，可以通过高效液相色谱进行直接分析。这些微乳液是两种不相容的液体（通常是油和水）的分散体，通过与极性和非极性组分相互作用的两亲性物质使其相容。在SFME测试和结果的临界分析后，只要加入足够数量的1-辛醇：1-丙醇（3:10 v/v），无论所研究的油水比如何，都可以观察到清晰均匀的微乳液。基于已建立的稳健工作范围，选择1:2（油：超纯水）比（% w/w）作为折衷条件，在保证与色谱系统相容性的同时，最大限度地减少药用油的使用。采用ZORBAX Eclipse Plus C18色谱柱，对色谱条件进行单因素优化，可在35℃条件下同时分析五种大麻素，包括大麻二酚（CBD）、大麻二酸（CBDA）、四氢大麻酚（THCB）、大麻酚（CBN）和四氢大麻酚（Δ9-THC）。方法采用等容洗脱，流动相为82:18 %v/v甲醇：超纯水（含0.15 ± 0.05 % v/v甲酸），流速1.0 mL min−1，进样量20 μL，荧光和紫外吸收检测器。该方案提供了灵敏度（LOD在ng mL−1），选择性和分离效率，与低成本，最小的废物产生和低能源需求相关联，符合绿色化学原则。

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引用次数: 0

Assessment of the serum and salivary free light chain levels in patients with proteinuria post cardiac surgery 心脏手术后蛋白尿患者血清和唾液游离轻链水平的评估

Journal of Pharmaceutical and Biomedical Analysis Open

Pub Date : 2025-06-01 Epub Date: 2025-02-01 DOI: 10.1016/j.jpbao.2025.100058

Moazaz Rashad Saed , Thikra Hasan Mathkor

Acute kidney injury (AKI) is a prevalent complication following cardiac surgery, affecting both short- and long-term survival rates. Proteinuria serves as an indicator of structural kidney damage and is increasingly recognized as a crucial marker for kidney disease and a risk factor for AKI. Free light chains (FLCs) are being investigated as potential markers for assessing the risk of kidney damage. This study aimed to validate the early detection of AKI by examining FLCs (kappa, lambda, and the kappa/lambda ratio) in serum and saliva samples. Clinical data were collected from 149 patients who underwent cardiac surgery in Baghdad, with 90 providing both saliva and serum samples for FLC evaluation. Patients were categorized based on the severity of proteinuria. Results showed that serum kappa levels were significantly elevated in patients with severe proteinuria compared to those with mild or no proteinuria. Conversely, salivary kappa levels were higher in patients with mild proteinuria than in those with severe or no proteinuria, while salivary lambda levels decreased as proteinuria severity increased. The rise in serum kappa may be attributed to immune system activation and kidney impairment. Both serum and salivary kappa demonstrated strong predictive capabilities for post-surgery AKI prognosis, outperforming lambda and the kappa/lambda ratio based on ROC analysis. The findings suggest that FLCs, particularly kappa, could be valuable in the early detection of AKI following cardiac surgery, highlighting their potential to enhance patient outcomes through proactive monitoring and intervention.

急性肾损伤（AKI）是心脏手术后常见的并发症，影响短期和长期生存率。蛋白尿作为结构性肾脏损害的指标，越来越被认为是肾脏疾病的重要标志和AKI的危险因素。游离轻链（FLCs）作为评估肾损害风险的潜在标记物正在被研究。本研究旨在通过检测血清和唾液样本中的FLCs （kappa、lambda和kappa/lambda比值）来验证AKI的早期检测。临床数据来自巴格达接受心脏手术的149例患者，其中90例提供唾液和血清样本用于FLC评估。根据蛋白尿的严重程度对患者进行分类。结果显示，与轻度或无蛋白尿患者相比，重度蛋白尿患者血清kappa水平显著升高。相反，轻度蛋白尿患者的唾液kappa水平高于严重或无蛋白尿患者，而唾液lambda水平随着蛋白尿严重程度的增加而下降。血清kappa升高可能与免疫系统激活和肾脏损害有关。血清和唾液kappa对术后AKI预后的预测能力均较强，优于lambda和基于ROC分析的kappa/lambda比值。研究结果表明，flc，特别是kappa，在心脏手术后AKI的早期检测中可能是有价值的，突出了它们通过主动监测和干预提高患者预后的潜力。

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引用次数: 0

Recent advancements in novel electrochemical sensors for disease-associated molecule determination 用于疾病相关分子测定的新型电化学传感器的最新进展

Journal of Pharmaceutical and Biomedical Analysis Open

Pub Date : 2025-06-01 Epub Date: 2025-01-03 DOI: 10.1016/j.jpbao.2025.100051

Seyda Nur Samanci , Goksu Ozcelikay-Akyildiz , S. Irem Kaya , Sibel A. Ozkan

Disease-associated molecules (biomarkers) provide clinically important information for diagnosing, monitoring, and treating diseases common in society, such as cancer, neurodegenerative, metabolic, and infectious diseases. Reliable sensor platforms are required for sensitive, rapid, and selective determination of these molecules in biological fluids. Electrochemical sensors are popular options used effectively to determine many analytes in complex matrices to meet this need. One of the key points in assay of these clinically important molecules is to obtain sensitive responses even at low concentrations. Therefore, integration with materials such as nanomaterials, composites, carbon-based materials, and molecularly imprinted polymers (MIPs) is widely applied to improve the performance of electrochemical sensors. Another essential expectation in determining disease-related molecules is the ability to perform point-of-care analysis with portable devices. This way, it is possible to obtain results that the patient can follow and convey to healthcare professionals without losing time. This review discusses the most current and novel electrochemical sensor approaches in disease-associated molecule determination. These studies were overviewed regarding the relevant disease, sensor type, electrochemical method, linear range, LOD, sample, and recovery values. This review aims to shed light on current studies in this field and provide researchers with a resource for future applications.

疾病相关分子（生物标志物）为诊断、监测和治疗社会常见疾病提供了重要的临床信息，如癌症、神经退行性疾病、代谢性疾病和传染病。需要可靠的传感器平台来灵敏、快速和选择性地测定生物流体中的这些分子。电化学传感器是一种流行的选择，可以有效地确定复杂矩阵中的许多分析物，以满足这一需求。检测这些临床重要分子的关键点之一是即使在低浓度下也能获得敏感反应。因此，与纳米材料、复合材料、碳基材料和分子印迹聚合物（MIPs）等材料的集成被广泛应用于提高电化学传感器的性能。确定疾病相关分子的另一个基本期望是能够使用便携式设备进行即时分析。这样，就有可能获得患者可以跟踪并传达给医疗保健专业人员的结果，而不会浪费时间。本文综述了最新的电化学传感器在疾病相关分子测定中的应用。综述了相关疾病、传感器类型、电化学方法、线性范围、LOD、样品和回收率等方面的研究。本文综述了该领域的研究现状，为今后的应用研究提供参考。

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引用次数: 0

Developments toward analysis of pesticides at residue levels among High Chlorophyll Containing Edible Leafy Plants – Sampling, QuEChERS and LC-MS/MS based analysis 高叶绿素食用叶类植物农药残留分析研究进展——取样、QuEChERS和LC-MS/MS分析

Journal of Pharmaceutical and Biomedical Analysis Open

Pub Date : 2025-06-01 Epub Date: 2025-02-12 DOI: 10.1016/j.jpbao.2025.100062

Vikrant Goel , Deepika Pandey , Sudeep Shukla

The usage of pesticides is crucial in maintaining the long-term health of plantations and boosting crop productivity. Nonetheless, their uncontrolled application has led to contamination of the environment, primarily affecting crops, soil, and water resources. Leafy plants rich in chlorophyll, classified as superfoods, have enhanced the quality of human life, however, they are prone to contamination with foreign contamination compounds known as xenobiotics, which pose a challenge for routine laboratory analysis. This paper reviews advancements among various sample preparation techniques evolving towards modern QuEChERS methodology and the features of the liquid chromatography mass spectroscopy technique that are useful for the assessment of pesticides from multiple classes at residue levels in such highly nutritious substances from High Chlorophyll Containing Edible Leafy Plants.

农药的使用对于维持种植园的长期健康和提高作物生产力至关重要。然而，它们不受控制的使用导致了环境污染，主要影响到作物、土壤和水资源。富含叶绿素的叶状植物被列为超级食物，提高了人类的生活质量，然而，它们容易受到外来污染化合物的污染，这对常规实验室分析构成了挑战。本文综述了向现代QuEChERS方法发展的各种样品制备技术的进展，以及液相色谱-质谱技术的特点，这些技术可用于评估高叶绿素食用叶植物中高营养物质中多类农药的残留水平。

引用次数: 0