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Experimental TET2 Clonal Hematopoiesis Predisposes to Renal Hypertension Through an Inflammasome-Mediated Mechanism. 实验性 TET2 克隆造血通过炎症体介导的机制易导致肾性高血压
IF 16.5 1区 医学 Q1 CARDIAC & CARDIOVASCULAR SYSTEMS Pub Date : 2024-10-11 Epub Date: 2024-09-05 DOI: 10.1161/CIRCRESAHA.124.324492
Ariel H Polizio, Lucila Marino, Kyung-Duk Min, Yoshimitsu Yura, Luca Rolauer, Jesse D Cochran, Megan A Evans, Eunbee Park, Heather Doviak, Emiri Miura-Yura, Miranda E Good, Abigail G Wolpe, Maria Grandoch, Brant E Isakson, Kenneth Walsh

Background: Hypertension incidence increases with age and represents one of the most prevalent risk factors for cardiovascular disease. Clonal events in the hematopoietic system resulting from somatic mutations in driver genes are prevalent in elderly individuals who lack overt hematologic disorders. This condition is referred to as age-related clonal hematopoiesis (CH), and it is a newly recognized risk factor for cardiovascular disease. It is not known whether CH and hypertension in the elderly are causally related and, if so, what are the mechanistic features.

Methods: A murine model of adoptive bone marrow transplantation was employed to examine the interplay between Tet2 (ten-eleven translocation methylcytosine dioxygenase 2) clonal hematopoiesis and hypertension.

Results: In this model, a subpressor dose of Ang II (angiotensin II) resulted in elevated systolic and diastolic blood pressure as early as 1 day after challenge. These conditions led to the expansion of Tet2-deficient proinflammatory monocytes and bone marrow progenitor populations. Tet2 deficiency promoted renal CCL5 (C-C motif ligand 5) chemokine expression and macrophage infiltration into the kidney. Consistent with macrophage involvement, Tet2 deficiency in myeloid cells promoted hypertension when mice were treated with a subpressor dose of Ang II. The hematopoietic Tet2-/- condition led to sodium retention, renal inflammasome activation, and elevated levels of IL (interleukin)-1β and IL-18. Analysis of the sodium transporters indicated NCC (sodium-chloride symporter) and NKCC2 (Na+-K+-Cl- cotransporter 2) activation at residues Thr53 and Ser105, respectively. Administration of the NLRP3 (NLR family pyrin domain containing 3) inflammasome inhibitor MCC950 reversed the hypertensive state, sodium retention, and renal transporter activation.

Conclusions: Tet2-mediated CH sensitizes mice to a hypertensive stimulus. Mechanistically, the expansion of hematopoietic Tet2-deficient cells promotes hypertension due to elevated renal immune cell infiltration and activation of the NLRP3 inflammasome, with consequences on sodium retention. These data indicate that carriers of TET2 CH could be at elevated risk for the development of hypertension and that immune modulators could be useful in treating hypertension in this patient population.

背景:高血压发病率随年龄增长而增加,是心血管疾病最普遍的危险因素之一。在没有明显血液病的老年人中,由于驱动基因的体细胞突变而导致的造血系统克隆事件非常普遍。这种情况被称为与年龄相关的克隆性造血(CH),是一种新近被确认的心血管疾病风险因素。目前尚不清楚老年克隆性造血和高血压是否有因果关系,如果有,其机理特征是什么:方法和结果:我们采用了一种小鼠收养性骨髓移植模型来研究 Tet2(十-十一易位甲基胞嘧啶二氧酶 2)CH 与高血压之间的相互作用。在该模型中,亚压剂量的 Ang II(血管紧张素 II)导致收缩压和舒张压升高,最早出现在挑战后 1 天。这些条件导致 Tet2 缺陷的促炎单核细胞和骨髓祖细胞扩增。Tet2缺陷促进了肾脏CCL5趋化因子的表达和巨噬细胞向肾脏的浸润。与巨噬细胞的参与相一致,当小鼠接受亚抑制剂量的 Ang II 治疗时,骨髓细胞中的 Tet2 缺失会促进高血压。造血Tet2-/-条件导致钠潴留、肾脏炎症小体激活以及IL(白细胞介素)-1β和IL-18水平升高。对钠转运体的分析表明,NCC(Na+-Cl- 共转运体)和 NKCC2 分别在 Thr53 和 Ser105 残基处被激活。服用 NLRP3 炎性体抑制剂 MCC950 逆转了高血压状态、钠潴留和肾转运体激活:结论:Tet2 介导的 CH 使小鼠对高血压刺激敏感。从机理上讲,造血Tet2缺陷细胞的扩增会促进肾脏免疫细胞浸润和NLRP3炎性体的激活,从而导致高血压,并对钠潴留产生影响。这些数据表明,TET2 CH携带者罹患高血压的风险可能会升高,免疫调节剂可能有助于治疗这类患者的高血压。
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引用次数: 0
Meet the First Authors. 认识第一作者
IF 16.5 1区 医学 Q1 CARDIAC & CARDIOVASCULAR SYSTEMS Pub Date : 2024-10-11 Epub Date: 2024-10-10 DOI: 10.1161/RES.0000000000000696
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引用次数: 0
Salt and CHIP: Tet2-CH Aggravates Salt-Sensitive Hypertension in Mice. 盐与 CHIP:Tet2-CH 会加重小鼠对盐敏感的高血压。
IF 16.5 1区 医学 Q1 CARDIAC & CARDIOVASCULAR SYSTEMS Pub Date : 2024-10-11 Epub Date: 2024-10-10 DOI: 10.1161/CIRCRESAHA.124.325364
Caitlyn Vlasschaert, Steven D Crowley, Alexander G Bick
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引用次数: 0
Chronic Activation of Tubulin Tyrosination Improves Heart Function. 管蛋白酪氨酸化的慢性激活可改善心脏功能
IF 16.5 1区 医学 Q1 CARDIAC & CARDIOVASCULAR SYSTEMS Pub Date : 2024-10-11 Epub Date: 2024-09-16 DOI: 10.1161/CIRCRESAHA.124.324387
Niels Pietsch, Christina Y Chen, Svenja Kupsch, Lucas Bacmeister, Birgit Geertz, Marisol Herrera-Rivero, Bente Siebels, Hannah Voß, Elisabeth Krämer, Ingke Braren, Dirk Westermann, Hartmut Schlüter, Giulia Mearini, Saskia Schlossarek, Jolanda van der Velden, Matthew A Caporizzo, Diana Lindner, Benjamin L Prosser, Lucie Carrier

Background: Hypertrophic cardiomyopathy (HCM) is the most common cardiac genetic disorder caused by sarcomeric gene variants and associated with left ventricular hypertrophy and diastolic dysfunction. The role of the microtubule network has recently gained interest with the findings that microtubule detyrosination (dTyr-MT) is markedly elevated in heart failure. Acute reduction of dTyr-MT by inhibition of the detyrosinase (VASH [vasohibin]/SVBP [small VASH-binding protein] complex) or activation of the tyrosinase (TTL [tubulin tyrosine ligase]) markedly improved contractility and reduced stiffness in human failing cardiomyocytes and thus posed a new perspective for HCM treatment. In this study, we tested the impact of chronic tubulin tyrosination in an HCM mouse model (Mybpc3 knock-in), in human HCM cardiomyocytes, and in SVBP-deficient human engineered heart tissues (EHTs).

Methods: Adeno-associated virus serotype 9-mediated TTL transfer was applied in neonatal wild-type rodents, in 3-week-old knock-in mice, and in HCM human induced pluripotent stem cell-derived cardiomyocytes.

Results: We show (1) TTL for 6 weeks dose dependently reduced dTyr-MT and improved contractility without affecting cytosolic calcium transients in wild-type cardiomyocytes; (2) TTL for 12 weeks reduced the abundance of dTyr-MT in the myocardium, improved diastolic filling, compliance, cardiac output, and stroke volume in knock-in mice; (3) TTL for 10 days normalized cell area in HCM human induced pluripotent stem cell-derived cardiomyocytes; (4) TTL overexpression activated transcription of tubulins and other cytoskeleton components but did not significantly impact the proteome in knock-in mice; (5) SVBP-deficient EHTs exhibited reduced dTyr-MT levels, higher force, and faster relaxation than TTL-deficient and wild-type EHTs. RNA sequencing and mass spectrometry analysis revealed distinct enrichment of cardiomyocyte components and pathways in SVBP-deficient versus TTL-deficient EHTs.

Conclusions: This study provides the first proof of concept that chronic activation of tubulin tyrosination in HCM mice and in human EHTs improves heart function and holds promise for targeting the nonsarcomeric cytoskeleton in heart disease.

理由:肥厚型心肌病(HCM)是由肉瘤基因变异引起的最常见的心脏遗传疾病,与左心室肥大和舒张功能障碍有关。最近,随着微管脱酪氨酸化(dTyr-MT)在心力衰竭中明显升高的发现,微管网络的作用引起了人们的兴趣。通过抑制脱酪氨酸酶(VASH[血管抑制素]/SVBP[小 VASH 结合蛋白]复合物)或激活酪氨酸酶(TTL[微管蛋白酪氨酸连接酶])来急性减少 dTyr-MT,可明显改善人类衰竭心肌细胞的收缩能力并降低僵硬度,从而为 HCM 治疗提供了新的视角:在这项研究中,我们测试了慢性小管蛋白酪氨酸化对 HCM 小鼠模型(Mybpc3 基因敲入)、人类 HCM 心肌细胞以及 SVBP 缺陷人类工程心脏组织(EHTs)的影响:在新生野生型啮齿类动物、3 周大的基因敲入小鼠和 HCM 人类诱导多能干细胞衍生心肌细胞中应用了腺相关病毒血清型 9 介导的 TTL 转移。我们发现:(1)TTL 6 周剂量依赖性地减少了野生型心肌细胞中的 dTyr-MT,并改善了收缩力,而不影响细胞钙瞬态;(2)TTL 12 周减少了基因敲入小鼠心肌中 dTyr-MT 的丰度,改善了舒张充盈、顺应性、心输出量和搏出量;(3) TTL 10 天能使 HCM 人诱导多能干细胞衍生心肌细胞的细胞面积正常化;(4) TTL 过表达能激活小管蛋白和其他细胞骨架成分的转录,但不会对基因敲入小鼠的蛋白质组产生显著影响;(5) 与 TTL 缺失型和野生型 EHT 相比,SVBP 缺失型 EHT 的 dTyr-MT 含量降低,力更大,松弛更快。RNA测序和质谱分析表明,SVBP缺陷型和TTL缺陷型EHT的心肌细胞成分和通路截然不同:这项研究首次证明了在 HCM 小鼠和人类 EHTs 中慢性激活微管蛋白酪氨酸化能改善心脏功能的概念,并为针对心脏病的非星形细胞骨架带来了希望。
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引用次数: 0
Arg92Leu-cTnT Alters the cTnC-cTnI Interface Disrupting PKA-Mediated Relaxation. Arg92Leu-cTnT 改变了 cTnC-cTnI 接口,破坏了 PKA 介导的松弛。
IF 20.1 1区 医学 Q1 CARDIAC & CARDIOVASCULAR SYSTEMS Pub Date : 2024-09-27 DOI: 10.1161/circresaha.124.325223
Melissa L Lynn,Jesus Jimenez,Romi L Castillo,Catherine Vasquez,Matthew M Klass,Anthony Baldo,Andrew Kim,Cyonna Gibson,Anne M Murphy,Jil C Tardiff
BACKGROUNDImpaired left ventricular relaxation, high filling pressures, and dysregulation of Ca2+ homeostasis are common findings contributing to diastolic dysfunction in hypertrophic cardiomyopathy (HCM). Studies have shown that impaired relaxation is an early observation in the sarcomere-gene-positive preclinical HCM cohort, which suggests the potential involvement of myofilament regulators in relaxation. A molecular-level understanding of mechanism(s) at the level of the myofilament is lacking. We hypothesized that mutation-specific, allosterically mediated, changes to the cTnC (cardiac troponin C)-cTnI (cardiac troponin I) interface can account for the development of early-onset diastolic dysfunction via decreased PKA accessibility to cTnI.METHODSHCM mutations R92L-cTnT (cardiac troponin T; Arg92Leu) and Δ160E-cTnT (Glu160 deletion) were studied in vivo, in vitro, and in silico via 2-dimensional echocardiography, Western blotting, ex vivo hemodynamics, stopped-flow kinetics, time-resolved fluorescence resonance energy transfer, and molecular dynamics simulations.RESULTSThe HCM-causative mutations R92L-cTnT and Δ160E-cTnT result in different time-of-onset diastolic dysfunction. R92L-cTnT demonstrated early-onset diastolic dysfunction accompanied by a localized decrease in phosphorylation of cTnI. Constitutive phosphorylation of cTnI (cTnI-D23D24) was sufficient to recover diastolic function to non-Tg levels only for R92L-cTnT. Mutation-specific changes in Ca2+ dissociation rates associated with R92L-cTnT reconstituted with cTnI-D23D24 led us to investigate potential involvement of structural changes in the cTnC-cTnI interface as an explanation for these observations. We probed the interface via time-resolved fluorescence resonance energy transfer revealing a repositioning of the N-terminus of cTnI, closer to cTnC, and concomitant decreases in distance distributions at sites flanking the PKA consensus sequence. Implementing time-resolved fluorescence resonance energy transfer distances as constraints into our atomistic model identified additional electrostatic interactions at the consensus sequence.CONCLUSIONSThese data show that the early diastolic dysfunction observed in a subset of HCM is attributable to allosterically mediated structural changes at the cTnC-cTnI interface that impair accessibility of PKA, thereby blunting β-adrenergic responsiveness and identifying a potential molecular target for therapeutic intervention.
背景左心室松弛功能受损、充盈压过高以及 Ca2+ 平衡失调是肥厚型心肌病 (HCM) 导致舒张功能障碍的常见原因。研究表明,在临床前 HCM 患者群中,肌节基因阳性的患者很早就发现松弛功能受损,这表明肌丝调节因子可能参与了松弛功能。目前还缺乏对肌丝水平机制的分子水平的了解。我们假设,由突变特异性异构体介导的 cTnC(心肌肌钙蛋白 C)-cTnI(心肌肌钙蛋白 I)界面变化可通过降低 PKA 对 cTnI 的可及性而导致早发舒张功能障碍。方法通过二维超声心动图、Western 印迹、体外血流动力学、停流动力学、时间分辨荧光共振能量转移和分子动力学模拟,对 HCM 基因突变 R92L-cTnT(心肌肌钙蛋白 T;Arg92Leu)和 Δ160E-cTnT(Glu160 缺失)进行了体内、体外和硅学研究。结果HCM致病突变 R92L-cTnT 和 Δ160E-cTnT导致舒张功能障碍的发病时间不同。R92L-cTnT 表现出早发性舒张功能障碍,并伴有局部 cTnI 磷酸化的降低。只有 R92L-cTnT 的 cTnI(cTnI-D23D24)持续磷酸化足以使舒张功能恢复到非 Tg 水平。与 cTnI-D23D24 重组的 R92L-cTnT 相关的 Ca2+ 解离率的突变特异性变化促使我们研究 cTnC-cTnI 界面结构变化的潜在参与,以解释这些观察结果。我们通过时间分辨荧光共振能量转移对界面进行了探测,发现 cTnI 的 N 端重新定位,更靠近 cTnC,同时 PKA 共识序列侧翼位点的距离分布减少。这些数据表明,在 HCM 亚群中观察到的早期舒张功能障碍可归因于 cTnC-cTnI 接口上异位介导的结构变化,这种变化损害了 PKA 的可及性,从而削弱了 β 肾上腺素能的反应性,并确定了一个潜在的治疗干预分子靶点。
{"title":"Arg92Leu-cTnT Alters the cTnC-cTnI Interface Disrupting PKA-Mediated Relaxation.","authors":"Melissa L Lynn,Jesus Jimenez,Romi L Castillo,Catherine Vasquez,Matthew M Klass,Anthony Baldo,Andrew Kim,Cyonna Gibson,Anne M Murphy,Jil C Tardiff","doi":"10.1161/circresaha.124.325223","DOIUrl":"https://doi.org/10.1161/circresaha.124.325223","url":null,"abstract":"BACKGROUNDImpaired left ventricular relaxation, high filling pressures, and dysregulation of Ca2+ homeostasis are common findings contributing to diastolic dysfunction in hypertrophic cardiomyopathy (HCM). Studies have shown that impaired relaxation is an early observation in the sarcomere-gene-positive preclinical HCM cohort, which suggests the potential involvement of myofilament regulators in relaxation. A molecular-level understanding of mechanism(s) at the level of the myofilament is lacking. We hypothesized that mutation-specific, allosterically mediated, changes to the cTnC (cardiac troponin C)-cTnI (cardiac troponin I) interface can account for the development of early-onset diastolic dysfunction via decreased PKA accessibility to cTnI.METHODSHCM mutations R92L-cTnT (cardiac troponin T; Arg92Leu) and Δ160E-cTnT (Glu160 deletion) were studied in vivo, in vitro, and in silico via 2-dimensional echocardiography, Western blotting, ex vivo hemodynamics, stopped-flow kinetics, time-resolved fluorescence resonance energy transfer, and molecular dynamics simulations.RESULTSThe HCM-causative mutations R92L-cTnT and Δ160E-cTnT result in different time-of-onset diastolic dysfunction. R92L-cTnT demonstrated early-onset diastolic dysfunction accompanied by a localized decrease in phosphorylation of cTnI. Constitutive phosphorylation of cTnI (cTnI-D23D24) was sufficient to recover diastolic function to non-Tg levels only for R92L-cTnT. Mutation-specific changes in Ca2+ dissociation rates associated with R92L-cTnT reconstituted with cTnI-D23D24 led us to investigate potential involvement of structural changes in the cTnC-cTnI interface as an explanation for these observations. We probed the interface via time-resolved fluorescence resonance energy transfer revealing a repositioning of the N-terminus of cTnI, closer to cTnC, and concomitant decreases in distance distributions at sites flanking the PKA consensus sequence. Implementing time-resolved fluorescence resonance energy transfer distances as constraints into our atomistic model identified additional electrostatic interactions at the consensus sequence.CONCLUSIONSThese data show that the early diastolic dysfunction observed in a subset of HCM is attributable to allosterically mediated structural changes at the cTnC-cTnI interface that impair accessibility of PKA, thereby blunting β-adrenergic responsiveness and identifying a potential molecular target for therapeutic intervention.","PeriodicalId":10147,"journal":{"name":"Circulation research","volume":"249 1","pages":""},"PeriodicalIF":20.1,"publicationDate":"2024-09-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142328759","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Crotonylation of NAE1 Modulates Cardiac Hypertrophy via Gelsolin Neddylation. NAE1 的 Crotonylation 通过 Gelsolin Neddylation 调节心肌肥大。
IF 16.5 1区 医学 Q1 CARDIAC & CARDIOVASCULAR SYSTEMS Pub Date : 2024-09-27 Epub Date: 2024-09-04 DOI: 10.1161/CIRCRESAHA.124.324733
Jie Ju, Kai Wang, Fang Liu, Cui-Yun Liu, Yun-Hong Wang, Shao-Cong Wang, Lu-Yu Zhou, Xin-Min Li, Yu-Qin Wang, Xin-Zhe Chen, Rui-Feng Li, Shi-Jun Xu, Chen Chen, Mei-Hua Zhang, Su-Min Yang, Jin-Wei Tian, Kun Wang
<p><strong>Background: </strong>Cardiac hypertrophy and its associated remodeling are among the leading causes of heart failure. Lysine crotonylation is a recently discovered posttranslational modification whose role in cardiac hypertrophy remains largely unknown. NAE1 (NEDD8 [neural precursor cell expressed developmentally downregulated protein 8]-activating enzyme E1 regulatory subunit) is mainly involved in the neddylation modification of protein targets. However, the function of crotonylated NAE1 has not been defined. This study aims to elucidate the effects and mechanisms of NAE1 crotonylation on cardiac hypertrophy.</p><p><strong>Methods: </strong>Crotonylation levels were detected in both human and mouse subjects with cardiac hypertrophy through immunoprecipitation and Western blot assays. Tandem mass tag (TMT)-labeled quantitative lysine crotonylome analysis was performed to identify the crotonylated proteins in a mouse cardiac hypertrophic model induced by transverse aortic constriction. We generated NAE1 knock-in mice carrying a crotonylation-defective K238R (lysine to arginine mutation at site 238) mutation (NAE1 K238R) and NAE1 knock-in mice expressing a crotonylation-mimicking K238Q (lysine to glutamine mutation at site 238) mutation (NAE1 K238Q) to assess the functional role of crotonylation of NAE1 at K238 in pathological cardiac hypertrophy. Furthermore, we combined coimmunoprecipitation, mass spectrometry, and dot blot analysis that was followed by multiple molecular biological methodologies to identify the target GSN (gelsolin) and corresponding molecular events contributing to the function of NAE1 K238 (lysine residue at site 238) crotonylation.</p><p><strong>Results: </strong>The crotonylation level of NAE1 was increased in mice and patients with cardiac hypertrophy. Quantitative crotonylomics analysis revealed that K238 was the main crotonylation site of NAE1. Loss of K238 crotonylation in NAE1 K238R knock-in mice attenuated cardiac hypertrophy and restored the heart function, while hypercrotonylation mimic in NAE1 K238Q knock-in mice significantly enhanced transverse aortic constriction-induced pathological hypertrophic response, leading to impaired cardiac structure and function. The recombinant adenoviral vector carrying NAE1 K238R mutant attenuated, while the K238Q mutant aggravated Ang II (angiotensin II)-induced hypertrophy. Mechanistically, we identified GSN as a direct target of NAE1. K238 crotonylation of NAE1 promoted GSN neddylation and, thus, enhanced its protein stability and expression. NAE1 crotonylation-dependent increase of GSN promoted actin-severing activity, which resulted in adverse cytoskeletal remodeling and progression of pathological hypertrophy.</p><p><strong>Conclusions: </strong>Our findings provide new insights into the previously unrecognized role of crotonylation on nonhistone proteins during cardiac hypertrophy. We found that K238 crotonylation of NAE1 plays an essential role in mediating cardi
背景:心脏肥大及其相关重塑是导致心力衰竭的主要原因之一。赖氨酸巴豆酰化是最近发现的一种翻译后修饰,其在心肌肥厚中的作用在很大程度上仍然未知。NAE1(NEDD8-激活酶 E1 调控亚基)主要参与蛋白质靶点的奈德基化修饰。然而,巴豆酰化的 NAE1 的功能尚未明确。本研究旨在阐明NAE1巴豆酰化对心脏肥大的影响和机制:方法:通过免疫沉淀和Western印迹检测人和小鼠心肌肥厚患者的巴豆酰化水平。在横向主动脉收缩诱导的小鼠心脏肥大模型中,进行了TMT标记的赖氨酸巴豆酰化定量分析,以确定巴豆酰化的蛋白质。我们产生了携带赖氨酸至精氨酸K238R(位点238上的赖氨酸至精氨酸突变)突变的NAE1基因敲入小鼠(NAE1 K238R)和表达模拟赖氨酸至谷氨酸突变的NAE1基因敲入小鼠(NAE1 K238R)。我们还研究了表达模拟赖氨酸至谷氨酰胺 K238Q(位点 238 上的赖氨酸至谷氨酰胺突变)突变(NAE1 K238Q)的 NAE1 基因敲入小鼠,以评估 NAE1 K238 上的巴豆酰化在病理性心肌肥厚中的功能作用。此外,我们还结合免疫沉淀、质谱分析和点印迹分析等多种分子生物学方法,鉴定了目标GSN(凝胶溶素)和导致NAE1 K238巴豆酰化功能的相应分子事件:结果:在小鼠和心肌肥厚患者体内,NAE1的巴豆酰化水平升高。定量巴豆酰组学分析表明,K238是NAE1的主要巴豆酰化位点。NAE1 K238R基因敲入小鼠的K238巴豆酰化缺失可减轻心脏肥大并恢复心脏功能,而NAE1 K238Q基因敲入小鼠的高巴豆酰化模拟可显著增强横主动脉收缩诱导的病理性肥大反应,导致心脏结构和功能受损。携带NAE1 K238R突变体的重组腺病毒载体减轻了Ang II(血管紧张素II)诱导的肥厚,而K238Q突变体则加重了Ang II诱导的肥厚。从机理上讲,我们发现GSN是NAE1的直接靶标。NAE1的K238巴豆酰化促进了GSN的尼达基化,从而增强了其蛋白的稳定性和表达。NAE1巴豆酰化依赖的GSN增加促进了肌动蛋白的分裂活性,从而导致了不良的细胞骨架重塑和病理性肥大的进展:我们的研究结果为我们提供了新的视角,使我们了解到巴豆酰化在心脏肥大过程中对非组蛋白的作用。我们发现,NAE1的K238巴豆酰化在通过GSN内酰化介导心肌肥大中起着至关重要的作用,这为病理性肥大和心脏重塑提供了潜在的新治疗靶点。
{"title":"Crotonylation of NAE1 Modulates Cardiac Hypertrophy via Gelsolin Neddylation.","authors":"Jie Ju, Kai Wang, Fang Liu, Cui-Yun Liu, Yun-Hong Wang, Shao-Cong Wang, Lu-Yu Zhou, Xin-Min Li, Yu-Qin Wang, Xin-Zhe Chen, Rui-Feng Li, Shi-Jun Xu, Chen Chen, Mei-Hua Zhang, Su-Min Yang, Jin-Wei Tian, Kun Wang","doi":"10.1161/CIRCRESAHA.124.324733","DOIUrl":"10.1161/CIRCRESAHA.124.324733","url":null,"abstract":"&lt;p&gt;&lt;strong&gt;Background: &lt;/strong&gt;Cardiac hypertrophy and its associated remodeling are among the leading causes of heart failure. Lysine crotonylation is a recently discovered posttranslational modification whose role in cardiac hypertrophy remains largely unknown. NAE1 (NEDD8 [neural precursor cell expressed developmentally downregulated protein 8]-activating enzyme E1 regulatory subunit) is mainly involved in the neddylation modification of protein targets. However, the function of crotonylated NAE1 has not been defined. This study aims to elucidate the effects and mechanisms of NAE1 crotonylation on cardiac hypertrophy.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Methods: &lt;/strong&gt;Crotonylation levels were detected in both human and mouse subjects with cardiac hypertrophy through immunoprecipitation and Western blot assays. Tandem mass tag (TMT)-labeled quantitative lysine crotonylome analysis was performed to identify the crotonylated proteins in a mouse cardiac hypertrophic model induced by transverse aortic constriction. We generated NAE1 knock-in mice carrying a crotonylation-defective K238R (lysine to arginine mutation at site 238) mutation (NAE1 K238R) and NAE1 knock-in mice expressing a crotonylation-mimicking K238Q (lysine to glutamine mutation at site 238) mutation (NAE1 K238Q) to assess the functional role of crotonylation of NAE1 at K238 in pathological cardiac hypertrophy. Furthermore, we combined coimmunoprecipitation, mass spectrometry, and dot blot analysis that was followed by multiple molecular biological methodologies to identify the target GSN (gelsolin) and corresponding molecular events contributing to the function of NAE1 K238 (lysine residue at site 238) crotonylation.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Results: &lt;/strong&gt;The crotonylation level of NAE1 was increased in mice and patients with cardiac hypertrophy. Quantitative crotonylomics analysis revealed that K238 was the main crotonylation site of NAE1. Loss of K238 crotonylation in NAE1 K238R knock-in mice attenuated cardiac hypertrophy and restored the heart function, while hypercrotonylation mimic in NAE1 K238Q knock-in mice significantly enhanced transverse aortic constriction-induced pathological hypertrophic response, leading to impaired cardiac structure and function. The recombinant adenoviral vector carrying NAE1 K238R mutant attenuated, while the K238Q mutant aggravated Ang II (angiotensin II)-induced hypertrophy. Mechanistically, we identified GSN as a direct target of NAE1. K238 crotonylation of NAE1 promoted GSN neddylation and, thus, enhanced its protein stability and expression. NAE1 crotonylation-dependent increase of GSN promoted actin-severing activity, which resulted in adverse cytoskeletal remodeling and progression of pathological hypertrophy.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Conclusions: &lt;/strong&gt;Our findings provide new insights into the previously unrecognized role of crotonylation on nonhistone proteins during cardiac hypertrophy. We found that K238 crotonylation of NAE1 plays an essential role in mediating cardi","PeriodicalId":10147,"journal":{"name":"Circulation research","volume":" ","pages":"806-821"},"PeriodicalIF":16.5,"publicationDate":"2024-09-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142124985","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Systems Biology Approach Uncovers Candidates for Liver-Heart Interorgan Crosstalk in HFpEF. 系统生物学方法揭示了高频心衰患者肝-心器官间串联的候选者。
IF 16.5 1区 医学 Q1 CARDIAC & CARDIOVASCULAR SYSTEMS Pub Date : 2024-09-27 Epub Date: 2024-08-29 DOI: 10.1161/CIRCRESAHA.124.324829
Stefano Strocchi, Luo Liu, Rongling Wang, Steffen P Häseli, Federico Capone, David Bode, Natasha Nambiar, Tolga Eroglu, Leandro Santiago Padilla, Catherine Farrelly, Antonio Vacca, Marianna Mascagni, Christian U Oeing, Ulrich Kintscher, Simone Jung, Saskia A Diezel, Sarah V Liévano Contreras, Mingqi Zhou, Marcus Seldin, Gabriele G Schiattarella
{"title":"Systems Biology Approach Uncovers Candidates for Liver-Heart Interorgan Crosstalk in HFpEF.","authors":"Stefano Strocchi, Luo Liu, Rongling Wang, Steffen P Häseli, Federico Capone, David Bode, Natasha Nambiar, Tolga Eroglu, Leandro Santiago Padilla, Catherine Farrelly, Antonio Vacca, Marianna Mascagni, Christian U Oeing, Ulrich Kintscher, Simone Jung, Saskia A Diezel, Sarah V Liévano Contreras, Mingqi Zhou, Marcus Seldin, Gabriele G Schiattarella","doi":"10.1161/CIRCRESAHA.124.324829","DOIUrl":"10.1161/CIRCRESAHA.124.324829","url":null,"abstract":"","PeriodicalId":10147,"journal":{"name":"Circulation research","volume":" ","pages":"873-876"},"PeriodicalIF":16.5,"publicationDate":"2024-09-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11427132/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142104845","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Blunted Cardiac Mitophagy in Response to Metabolic Stress Contributes to HFpEF. 心脏对代谢压力的有丝分裂反应迟钝是导致高房血症的原因之一。
IF 20.1 1区 医学 Q1 CARDIAC & CARDIOVASCULAR SYSTEMS Pub Date : 2024-09-27 DOI: 10.1161/circresaha.123.324103
Akira Yoshii,Timothy S McMillen,Yajun Wang,Bo Zhou,Hongye Chen,Durba Banerjee,Melisa Herrero,Pei Wang,Naoto Muraoka,Wang Wang,Charles E Murry,Rong Tian
BACKGROUNDMetabolic remodeling and mitochondrial dysfunction are hallmarks of heart failure with reduced ejection fraction. However, their role in the pathogenesis of HF with preserved ejection fraction (HFpEF) is poorly understood.METHODSIn a mouse model of HFpEF, induced by high-fat diet and Nω-nitrol-arginine methyl ester, cardiac energetics was measured by 31P NMR spectroscopy and substrate oxidation profile was assessed by 13C-isotopmer analysis. Mitochondrial functions were assessed in the heart tissue and human induced pluripotent stem cell-derived cardiomyocytes.RESULTSHFpEF hearts presented a lower phosphocreatine content and a reduced phosphocreatine/ATP ratio, similar to that in heart failure with reduced ejection fraction. Decreased respiratory function and increased reactive oxygen species production were observed in mitochondria isolated from HFpEF hearts suggesting mitochondrial dysfunction. Cardiac substrate oxidation profile showed a high dependency on fatty acid oxidation in HFpEF hearts, which is the opposite of heart failure with reduced ejection fraction but similar to that in high-fat diet hearts. However, phosphocreatine/ATP ratio and mitochondrial function were sustained in the high-fat diet hearts. We found that mitophagy was activated in the high-fat diet heart but not in HFpEF hearts despite similar extent of obesity suggesting that mitochondrial quality control response was impaired in HFpEF hearts. Using a human induced pluripotent stem cell-derived cardiomyocyte mitophagy reporter, we found that fatty acid loading stimulated mitophagy, which was obliterated by inhibiting fatty acid oxidation. Enhancing fatty acid oxidation by deleting ACC2 (acetyl-CoA carboxylase 2) in the heart stimulated mitophagy and improved HFpEF phenotypes.CONCLUSIONSMaladaptation to metabolic stress in HFpEF hearts impairs mitochondrial quality control and contributed to the pathogenesis, which can be improved by stimulating fatty acid oxidation.
背景代谢重塑和线粒体功能障碍是射血分数降低型心力衰竭的特征。方法在高脂饮食和 Nω-硝基精氨酸甲酯诱导的高射血分数心力衰竭小鼠模型中,通过 31P NMR 光谱测定心脏能量,并通过 13C 同位素分析评估底物氧化概况。结果HFpEF心脏的磷酸肌酸含量较低,磷酸肌酸/ATP比率降低,与射血分数降低的心力衰竭相似。从高频低氧血症心脏分离的线粒体中观察到呼吸功能下降,活性氧生成增加,表明线粒体功能障碍。心脏底物氧化谱显示,HFpEF 心脏高度依赖脂肪酸氧化,这与射血分数降低的心衰相反,但与高脂饮食心脏相似。然而,高脂饮食心脏的磷酸肌酸/ATP比率和线粒体功能得以维持。我们发现,尽管肥胖程度相似,但高脂饮食心脏中的有丝分裂被激活,而高密度脂蛋白EF心脏中的有丝分裂没有被激活,这表明高密度脂蛋白EF心脏中的线粒体质量控制反应受损。通过使用人类诱导多能干细胞衍生的心肌细胞有丝分裂报告物,我们发现脂肪酸负荷会刺激有丝分裂,而抑制脂肪酸氧化则会消除这种现象。结论HFpEF心脏对代谢应激的不适应会损害线粒体质量控制并导致发病,而促进脂肪酸氧化则可改善这一问题。
{"title":"Blunted Cardiac Mitophagy in Response to Metabolic Stress Contributes to HFpEF.","authors":"Akira Yoshii,Timothy S McMillen,Yajun Wang,Bo Zhou,Hongye Chen,Durba Banerjee,Melisa Herrero,Pei Wang,Naoto Muraoka,Wang Wang,Charles E Murry,Rong Tian","doi":"10.1161/circresaha.123.324103","DOIUrl":"https://doi.org/10.1161/circresaha.123.324103","url":null,"abstract":"BACKGROUNDMetabolic remodeling and mitochondrial dysfunction are hallmarks of heart failure with reduced ejection fraction. However, their role in the pathogenesis of HF with preserved ejection fraction (HFpEF) is poorly understood.METHODSIn a mouse model of HFpEF, induced by high-fat diet and Nω-nitrol-arginine methyl ester, cardiac energetics was measured by 31P NMR spectroscopy and substrate oxidation profile was assessed by 13C-isotopmer analysis. Mitochondrial functions were assessed in the heart tissue and human induced pluripotent stem cell-derived cardiomyocytes.RESULTSHFpEF hearts presented a lower phosphocreatine content and a reduced phosphocreatine/ATP ratio, similar to that in heart failure with reduced ejection fraction. Decreased respiratory function and increased reactive oxygen species production were observed in mitochondria isolated from HFpEF hearts suggesting mitochondrial dysfunction. Cardiac substrate oxidation profile showed a high dependency on fatty acid oxidation in HFpEF hearts, which is the opposite of heart failure with reduced ejection fraction but similar to that in high-fat diet hearts. However, phosphocreatine/ATP ratio and mitochondrial function were sustained in the high-fat diet hearts. We found that mitophagy was activated in the high-fat diet heart but not in HFpEF hearts despite similar extent of obesity suggesting that mitochondrial quality control response was impaired in HFpEF hearts. Using a human induced pluripotent stem cell-derived cardiomyocyte mitophagy reporter, we found that fatty acid loading stimulated mitophagy, which was obliterated by inhibiting fatty acid oxidation. Enhancing fatty acid oxidation by deleting ACC2 (acetyl-CoA carboxylase 2) in the heart stimulated mitophagy and improved HFpEF phenotypes.CONCLUSIONSMaladaptation to metabolic stress in HFpEF hearts impairs mitochondrial quality control and contributed to the pathogenesis, which can be improved by stimulating fatty acid oxidation.","PeriodicalId":10147,"journal":{"name":"Circulation research","volume":"1 1","pages":""},"PeriodicalIF":20.1,"publicationDate":"2024-09-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142328681","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Meet the First Authors. 认识第一作者
IF 16.5 1区 医学 Q1 CARDIAC & CARDIOVASCULAR SYSTEMS Pub Date : 2024-09-27 Epub Date: 2024-09-26 DOI: 10.1161/RES.0000000000000694
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引用次数: 0
Monocytes Reprogrammed by 4-PBA Potently Contribute to the Resolution of Inflammation and Atherosclerosis. 经 4-PBA 重编程的单核细胞可有效缓解炎症和动脉粥样硬化。
IF 16.5 1区 医学 Q1 CARDIAC & CARDIOVASCULAR SYSTEMS Pub Date : 2024-09-27 Epub Date: 2024-09-03 DOI: 10.1161/CIRCRESAHA.124.325023
Shuo Geng, Ran Lu, Yao Zhang, Yajun Wu, Ling Xie, Blake A Caldwell, Kisha Pradhan, Ziyue Yi, Jacqueline Hou, Feng Xu, Xian Chen, Liwu Li

Background: Chronic inflammation initiated by inflammatory monocytes underlies the pathogenesis of atherosclerosis. However, approaches that can effectively resolve chronic low-grade inflammation targeting monocytes are not readily available. The small chemical compound 4-phenylbutyric acid (4-PBA) exhibits broad anti-inflammatory effects in reducing atherosclerosis. Selective delivery of 4-PBA reprogrammed monocytes may hold novel potential in providing targeted and precision therapeutics for the treatment of atherosclerosis.

Methods: Systems analyses integrating single-cell RNA sequencing and complementary immunologic approaches characterized key resolving characteristics as well as defining markers of reprogrammed monocytes trained by 4-PBA. Molecular mechanisms responsible for monocyte reprogramming were assessed by integrated biochemical and genetic approaches. The intercellular propagation of homeostasis resolution was evaluated by coculture assays with donor monocytes trained by 4-PBA and recipient naive monocytes. The in vivo effects of monocyte resolution and atherosclerosis prevention by 4-PBA were assessed with the high-fat diet-fed ApoE-/- mouse model with IP 4-PBA administration. Furthermore, the selective efficacy of 4-PBA-trained monocytes was examined by IV transfusion of ex vivo trained monocytes by 4-PBA into recipient high-fat diet-fed ApoE-/- mice.

Results: In this study, we found that monocytes can be potently reprogrammed by 4-PBA into an immune-resolving state characterized by reduced adhesion and enhanced expression of anti-inflammatory mediator CD24. Mechanistically, 4-PBA reduced the expression of ICAM-1 (intercellular adhesion molecule 1) via reducing peroxisome stress and attenuating SYK (spleen tyrosine kinase)-mTOR (mammalian target of rapamycin) signaling. Concurrently, 4-PBA enhanced the expression of resolving mediator CD24 through promoting PPARγ (peroxisome proliferator-activated receptor γ) neddylation mediated by TOLLIP (toll-interacting protein). 4-PBA-trained monocytes can effectively propagate anti-inflammation activity to neighboring monocytes through CD24. Our data further demonstrated that 4-PBA-trained monocytes effectively reduce atherosclerosis pathogenesis when administered in vivo.

Conclusions: Our study describes a robust and effective approach to generate resolving monocytes, characterizes novel mechanisms for targeted monocyte reprogramming, and offers a precision therapeutics for atherosclerosis based on delivering reprogrammed resolving monocytes.

背景:由炎症单核细胞引发的慢性炎症是动脉粥样硬化发病机制的基础。然而,目前还没有针对单核细胞有效解决慢性低度炎症的方法。小分子化合物 4-苯基丁酸(4-PBA)在减轻动脉粥样硬化方面具有广泛的抗炎作用。选择性递送 4-PBA 重编程单核细胞可能具有新的潜力,为治疗动脉粥样硬化提供靶向和精准治疗:方法:结合单细胞 RNA 测序和互补免疫学方法进行系统分析,确定了 4-PBA 训练的重编程单核细胞的关键分辨特征和定义标记。综合生化和遗传方法评估了单核细胞重编程的分子机制。通过与经 4-PBA 训练的供体单核细胞和受体天真单核细胞进行共培养实验,评估了细胞间平衡解析的传播。在高脂饮食喂养的载脂蛋白E-/-小鼠模型中,通过IP 4-PBA给药,评估了4-PBA在体内解决单核细胞问题和预防动脉粥样硬化的效果。此外,还通过向接受高脂饮食的载脂蛋白E-/-小鼠静脉注射4-PBA训练的单核细胞,检测了4-PBA训练的单核细胞的选择性功效:结果:在这项研究中,我们发现单核细胞可被 4-PBA 有效地重塑为一种以粘附性降低和抗炎介质 CD24 表达增强为特征的免疫溶解状态。从机理上讲,4-PBA 通过降低过氧化物酶体应激和减弱 SYK(脾脏酪氨酸激酶)-mTOR(哺乳动物雷帕霉素靶标)信号传导,减少了 ICAM-1(细胞间粘附分子 1)的表达。与此同时,4-PBA 通过促进由 TOLLIP(通行费互作蛋白)介导的 PPARγ(过氧化物酶体增殖激活受体γ)内酰化,增强了分解介质 CD24 的表达。经过 4-PBA 训练的单核细胞可通过 CD24 向邻近的单核细胞有效传播抗炎活性。我们的数据进一步证明,4-PBA 训练的单核细胞在体内给药时可有效减少动脉粥样硬化的发病机制:我们的研究描述了一种稳健有效的方法来生成抗动脉粥样硬化单核细胞,描述了靶向单核细胞重编程的新机制,并提供了一种基于提供重编程抗动脉粥样硬化单核细胞的精准疗法。
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引用次数: 0
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Circulation research
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