Pub Date : 2020-07-13DOI: 10.11606/issn.2357-8041.clrd.2020.164731
Juliana Dias Aguiar, M. Youssef, I. S. Medeiros
A carie secundaria e a principal causa de falha da restauracao. Assim, o desenvolvimento de adesivos com acao antimicrobiana e uma opcao vantajosa para sua inibicao. No entanto, esse efeito deve ser comprovado, bem como que o beneficio adicional nao interfere nas propriedades mecânicas do material ou na biocompatibilidade. Analisamos adesivos com acao antimicrobiana por meio de testes microbiologicos, resistencia de uniao, grau de conversao e citotoxicidade. Analisamos 32 estudos com adesivos antimicrobianos disponiveis comercialmente (Clearfil ™ SE Protect Bond / MDPB, Gluma 2Bond / glutaraldeido, Peak Universal Bond / clorexidina) e materiais experimentais ou adesivos comerciais modificados com agentes antimicrobianos, incluindo materiais com metacrilato de amonio quaternario (QAM) [metacrilato de dimetilaminododecil (DMADDM) e metacrilato de dimetilaminohexadecil (DMAHDM)], nanoparticulas [prata (NAg), dioxido de titânio (TiO2), oxido de zinco (ZnO)], vidro bioativo dopado com prata ou zinco (BAG), titânio, iodo de titânio, cobre e compostos como triclosan, quercetina, extrato de semente de uva, entre outros. O uso de agentes antimicrobianos e uma perspectiva favoravel para a funcionalizacao de sistemas adesivos para inibir a carie secundaria. No entanto, mais estudos clinicos precisam provar a eficacia desses materiais.
{"title":"Adhesive systems modified with antimicrobial agents: a literature review","authors":"Juliana Dias Aguiar, M. Youssef, I. S. Medeiros","doi":"10.11606/issn.2357-8041.clrd.2020.164731","DOIUrl":"https://doi.org/10.11606/issn.2357-8041.clrd.2020.164731","url":null,"abstract":"A carie secundaria e a principal causa de falha da restauracao. Assim, o desenvolvimento de adesivos com acao antimicrobiana e uma opcao vantajosa para sua inibicao. No entanto, esse efeito deve ser comprovado, bem como que o beneficio adicional nao interfere nas propriedades mecânicas do material ou na biocompatibilidade. Analisamos adesivos com acao antimicrobiana por meio de testes microbiologicos, resistencia de uniao, grau de conversao e citotoxicidade. Analisamos 32 estudos com adesivos antimicrobianos disponiveis comercialmente (Clearfil ™ SE Protect Bond / MDPB, Gluma 2Bond / glutaraldeido, Peak Universal Bond / clorexidina) e materiais experimentais ou adesivos comerciais modificados com agentes antimicrobianos, incluindo materiais com metacrilato de amonio quaternario (QAM) [metacrilato de dimetilaminododecil (DMADDM) e metacrilato de dimetilaminohexadecil (DMAHDM)], nanoparticulas [prata (NAg), dioxido de titânio (TiO2), oxido de zinco (ZnO)], vidro bioativo dopado com prata ou zinco (BAG), titânio, iodo de titânio, cobre e compostos como triclosan, quercetina, extrato de semente de uva, entre outros. O uso de agentes antimicrobianos e uma perspectiva favoravel para a funcionalizacao de sistemas adesivos para inibir a carie secundaria. No entanto, mais estudos clinicos precisam provar a eficacia desses materiais.","PeriodicalId":10204,"journal":{"name":"Clinical and Laboratorial Research in Dentistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2020-07-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85945809","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-06-17DOI: 10.11606/issn.2357-8041.clrd.2020.168469
C. Cotomacio, Wladimir Gushiken de Campos, C. Lemos, F. Alves
Background. Dental implants are used to rehabilitate missing teeth, and despite high success rates, failures are inevitable. Ostensibly avoidable human errors in planning or technique can contribute to implant failure. Case presentation. Herein, we report a case of an unsuccessful dental implant surgery due to an inadequate surgical technique and bone exposure in a 54-year-old healthy woman. Conclusions. This report underscores the relevance of reporting the consequences of these human factors, such as loss of the implant, alveolar bone segment, and three teeth.
{"title":"Bone sequestration after dental implant surgery","authors":"C. Cotomacio, Wladimir Gushiken de Campos, C. Lemos, F. Alves","doi":"10.11606/issn.2357-8041.clrd.2020.168469","DOIUrl":"https://doi.org/10.11606/issn.2357-8041.clrd.2020.168469","url":null,"abstract":"Background. Dental implants are used to rehabilitate missing teeth, and despite high success rates, failures are inevitable. Ostensibly avoidable human errors in planning or technique can contribute to implant failure. Case presentation. Herein, we report a case of an unsuccessful dental implant surgery due to an inadequate surgical technique and bone exposure in a 54-year-old healthy woman. Conclusions. This report underscores the relevance of reporting the consequences of these human factors, such as loss of the implant, alveolar bone segment, and three teeth.","PeriodicalId":10204,"journal":{"name":"Clinical and Laboratorial Research in Dentistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2020-06-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76669787","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-06-16DOI: 10.11606/issn.2357-8041.clrd.2020.165832
Natalia Kecia Barbosa de Lima, Marilia de Lima Soares
Este trabalho aborda brevemente o processo de envelhecimento facial para revisar e discutir a utilização dos bioestimuladores de colágeno empregados na harmonização orofacial visando o rejuvenescimento da face. Método: Foi pesquisado nas bases SciELO, BIREME e PubMed por artigos publicados até 2019, nos idiomas inglês e português. Os critérios de inclusão englobaram artigos originais que analisaram ou discutiram sobre os preenchedores cutâneos com capacidade bioestimuladora e o processo de envelhecimento facial; no total,72 artigos foram considerados elegíveis para esta revisão de literatura. Discussão: No mercado dermatológico existem quatro preenchedores cutâneos (ácido poli-L-láctico, hidroxiapatita de cálcio, policaprolactona e polimetilmetacrilato) que possuem a faculdade de estimular a neocolagênese a partir de uma resposta inflamatória subclínica localizada por parte do corpo do paciente. Cada produto possui suas particularidades quanto à composição, o tempo de início do efeito, e a durabilidade. De acordo com a literatura, todos os preenchedores cutâneos são considerados eficazes e seguros, podendo ser utilizados por profissionais devidamente capacitados. Conclusão: mesmo os bioestimuladores de colágeno sendo excelentes materiais capazes de prevenir ou reverter os efeitos do envelhecimento facial, não existe, porém, um bioestimulador dérmico perfeito, pois todos podem gerar efeitos adversos, devendo o profissional saber selecionar o produto ideal para o tratamento de cada paciente.
{"title":"Utilização dos bioestimuladores de colágeno na harmonização orofacial","authors":"Natalia Kecia Barbosa de Lima, Marilia de Lima Soares","doi":"10.11606/issn.2357-8041.clrd.2020.165832","DOIUrl":"https://doi.org/10.11606/issn.2357-8041.clrd.2020.165832","url":null,"abstract":"Este trabalho aborda brevemente o processo de envelhecimento facial para revisar e discutir a utilização dos bioestimuladores de colágeno empregados na harmonização orofacial visando o rejuvenescimento da face. Método: Foi pesquisado nas bases SciELO, BIREME e PubMed por artigos publicados até 2019, nos idiomas inglês e português. Os critérios de inclusão englobaram artigos originais que analisaram ou discutiram sobre os preenchedores cutâneos com capacidade bioestimuladora e o processo de envelhecimento facial; no total,72 artigos foram considerados elegíveis para esta revisão de literatura. Discussão: No mercado dermatológico existem quatro preenchedores cutâneos (ácido poli-L-láctico, hidroxiapatita de cálcio, policaprolactona e polimetilmetacrilato) que possuem a faculdade de estimular a neocolagênese a partir de uma resposta inflamatória subclínica localizada por parte do corpo do paciente. Cada produto possui suas particularidades quanto à composição, o tempo de início do efeito, e a durabilidade. De acordo com a literatura, todos os preenchedores cutâneos são considerados eficazes e seguros, podendo ser utilizados por profissionais devidamente capacitados. Conclusão: mesmo os bioestimuladores de colágeno sendo excelentes materiais capazes de prevenir ou reverter os efeitos do envelhecimento facial, não existe, porém, um bioestimulador dérmico perfeito, pois todos podem gerar efeitos adversos, devendo o profissional saber selecionar o produto ideal para o tratamento de cada paciente.","PeriodicalId":10204,"journal":{"name":"Clinical and Laboratorial Research in Dentistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2020-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78013722","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-04-02DOI: 10.11606/issn.2357-8041.clrd.2020.162971
Erika Michele dos Santos Araújo, Bárbara Fávero Araújo Lima, Júlia Gomes Lúcio de Araújo, Fernanda Cristina Nogueira Rodrigues, Stella-Ferreira Amaral, Andréa Dias Neves Lago
Objective: This case report proposes a treatment for dentin hypersensitivity (DH) using photobiomodulation (PBT) with low power diode laser. Methods and Results: Male patient, 28 years old, reporting “dental sensitivity,” diagnosed by anamnesis and intraoral examination, with non-carious cervical lesions (NCCL) and DH on teeth 15 to 25, with different pain intensities, measured with visual analogue scale (VAS). For DH treatment, a PBT was proposed, with 808nm, 100mW, 20s and 2J of energy, applied during 3 sessions, with one-week interval and reevaluation after 30 days. After the first session, the patient reported improvement of sensitivity in all teeth, except for 15, that remained sensitive even during the reevaluation. Conclusion: PBT was effective in DH treatment, with desensitization being observed for 30 days in 90% of treated teeth.
{"title":"The effects of photobiomodulation therapy for the treatment of dentin hypersensitivity","authors":"Erika Michele dos Santos Araújo, Bárbara Fávero Araújo Lima, Júlia Gomes Lúcio de Araújo, Fernanda Cristina Nogueira Rodrigues, Stella-Ferreira Amaral, Andréa Dias Neves Lago","doi":"10.11606/issn.2357-8041.clrd.2020.162971","DOIUrl":"https://doi.org/10.11606/issn.2357-8041.clrd.2020.162971","url":null,"abstract":"Objective: This case report proposes a treatment for dentin hypersensitivity (DH) using photobiomodulation (PBT) with low power diode laser. Methods and Results: Male patient, 28 years old, reporting “dental sensitivity,” diagnosed by anamnesis and intraoral examination, with non-carious cervical lesions (NCCL) and DH on teeth 15 to 25, with different pain intensities, measured with visual analogue scale (VAS). For DH treatment, a PBT was proposed, with 808nm, 100mW, 20s and 2J of energy, applied during 3 sessions, with one-week interval and reevaluation after 30 days. After the first session, the patient reported improvement of sensitivity in all teeth, except for 15, that remained sensitive even during the reevaluation. Conclusion: PBT was effective in DH treatment, with desensitization being observed for 30 days in 90% of treated teeth.","PeriodicalId":10204,"journal":{"name":"Clinical and Laboratorial Research in Dentistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2020-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84749183","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-03-19DOI: 10.11606/issn.2357-8041.clrd.2020.160816
Simone Kreve, Gabriela Carolina D'Ávila, Letícia Oliveira dos Santos, A. C. Reis
Objetivo: Avaliou-se a autopercepção das condições de saúde bucal de um grupo de idosos por meio da aplicação do índice General Oral Health Assessment Index (Gohai). Métodos: Participaram do estudo 136 idosos, frequentadores do Centro de Revitalização da Terceira Idade (Certi), onde aplicou-se um questionário com questões do índice Gohai sobre a autopercepção das condições bucais. Realizou-se análise descritiva do resultado de cada uma das perguntas. Resultados: Dos idosos entrevistados, 78,7% responderam sempre sentir dor ou desconforto ao deglutir; 55,1% relataram desconforto em comer algum alimento, e 66,9% relataram insatisfação com a aparência da sua boca. A média global do índice Gohai quando aplicada à escala de interpretação foi de 28,88, classificada com uma percepção de saúde bucal “ruim” sobre a qualidade de vida. Conclusão: Os idosos avaliaram negativamente suas condições bucais pelo desconforto em comer, dificuldade/dor para deglutir e insatisfação com sua condição bucal.
{"title":"Autopercepção da saúde bucal de idosos","authors":"Simone Kreve, Gabriela Carolina D'Ávila, Letícia Oliveira dos Santos, A. C. Reis","doi":"10.11606/issn.2357-8041.clrd.2020.160816","DOIUrl":"https://doi.org/10.11606/issn.2357-8041.clrd.2020.160816","url":null,"abstract":"Objetivo: Avaliou-se a autopercepção das condições de saúde bucal de um grupo de idosos por meio da aplicação do índice General Oral Health Assessment Index (Gohai). Métodos: Participaram do estudo 136 idosos, frequentadores do Centro de Revitalização da Terceira Idade (Certi), onde aplicou-se um questionário com questões do índice Gohai sobre a autopercepção das condições bucais. Realizou-se análise descritiva do resultado de cada uma das perguntas. Resultados: Dos idosos entrevistados, 78,7% responderam sempre sentir dor ou desconforto ao deglutir; 55,1% relataram desconforto em comer algum alimento, e 66,9% relataram insatisfação com a aparência da sua boca. A média global do índice Gohai quando aplicada à escala de interpretação foi de 28,88, classificada com uma percepção de saúde bucal “ruim” sobre a qualidade de vida. Conclusão: Os idosos avaliaram negativamente suas condições bucais pelo desconforto em comer, dificuldade/dor para deglutir e insatisfação com sua condição bucal.","PeriodicalId":10204,"journal":{"name":"Clinical and Laboratorial Research in Dentistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2020-03-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86751305","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-03-12DOI: 10.11606/issn.2357-8041.clrd.2020.164723
Wellington H Yanaguizawa, Solange Kobayashi Velasco, R. Petersen, F. Alves, M. Cavalcanti
Osteonecrosis occurs by cellular death of the bone tissue due to an irreversible external factor. This disorder may be clinically unidentified in the early stages and result in degradation of the bone architectural structure, leading to pain, bone destruction, and loss of function. Thus, imaging exams become relevant to the recognition and definition of the bone necroses. The aim of this study was to review the literature on imaging exams frequently used for the diagnosis and evaluation of patients undergoing drug therapies associated with osteonecrosis of the jaw, such as panoramic radiography, computed tomography, magnetic resonance imaging and bone scintigraphy.
{"title":"Imaging modalities in medication-related osteonecrosis of the jaw","authors":"Wellington H Yanaguizawa, Solange Kobayashi Velasco, R. Petersen, F. Alves, M. Cavalcanti","doi":"10.11606/issn.2357-8041.clrd.2020.164723","DOIUrl":"https://doi.org/10.11606/issn.2357-8041.clrd.2020.164723","url":null,"abstract":"Osteonecrosis occurs by cellular death of the bone tissue due to an irreversible external factor. This disorder may be clinically unidentified in the early stages and result in degradation of the bone architectural structure, leading to pain, bone destruction, and loss of function. Thus, imaging exams become relevant to the recognition and definition of the bone necroses. The aim of this study was to review the literature on imaging exams frequently used for the diagnosis and evaluation of patients undergoing drug therapies associated with osteonecrosis of the jaw, such as panoramic radiography, computed tomography, magnetic resonance imaging and bone scintigraphy.","PeriodicalId":10204,"journal":{"name":"Clinical and Laboratorial Research in Dentistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2020-03-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89398370","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-03-10DOI: 10.11606/issn.2357-8041.clrd.2020.161228
João Victor Silva Lira, Ana Carolina Pereira Gomes, Gabriella Rodovalho Paiva, Andrea Boscolo Valentim Salge, Sergio Massayuki Nakamura, D. T. D. Castro
A redução da dimensão vertical de oclusão (DVO) é consequência de um desequilíbrio oclusal que ocorre após a perda dos dentes. O restabelecimento da relação maxilomandibular é indispensável para o sucesso da reabilitação oral, devolvendo, assim, a estética, a função perdida e a qualidade de vida ao paciente. Uma alternativa de tratamento simples, econômica e de tempo reduzido para pacientes parcialmente desdentados que apresentam alterações no plano oclusal e redução da DVO é a overlay, um tipo de prótese que busca apoio na face oclusal de um ou mais dentes. Este estudo relata um caso clínico realizado durante o curso de graduação em Odontologia, na Policlínica Getúlio Vargas da Universidade de Uberaba, demonstrando a importância e a viabilidade técnica de prótese parcial removível com macroapoio no restabelecimento da DVO do paciente. A partir do que foi encontrado na literatura e no caso clínico relatado, pode-se concluir que as próteses parciais removíveis com macroapoios representam uma alternativa importante no tratamento de pacientes com redução da DVO e consequente restabelecimento das funções do sistema estomatognático, com aspectos conservadores e de reversibilidade.
{"title":"Uso de macroapoio para restabelecimento da dimensão vertical de oclusão","authors":"João Victor Silva Lira, Ana Carolina Pereira Gomes, Gabriella Rodovalho Paiva, Andrea Boscolo Valentim Salge, Sergio Massayuki Nakamura, D. T. D. Castro","doi":"10.11606/issn.2357-8041.clrd.2020.161228","DOIUrl":"https://doi.org/10.11606/issn.2357-8041.clrd.2020.161228","url":null,"abstract":"A redução da dimensão vertical de oclusão (DVO) é consequência de um desequilíbrio oclusal que ocorre após a perda dos dentes. O restabelecimento da relação maxilomandibular é indispensável para o sucesso da reabilitação oral, devolvendo, assim, a estética, a função perdida e a qualidade de vida ao paciente. Uma alternativa de tratamento simples, econômica e de tempo reduzido para pacientes parcialmente desdentados que apresentam alterações no plano oclusal e redução da DVO é a overlay, um tipo de prótese que busca apoio na face oclusal de um ou mais dentes. Este estudo relata um caso clínico realizado durante o curso de graduação em Odontologia, na Policlínica Getúlio Vargas da Universidade de Uberaba, demonstrando a importância e a viabilidade técnica de prótese parcial removível com macroapoio no restabelecimento da DVO do paciente. A partir do que foi encontrado na literatura e no caso clínico relatado, pode-se concluir que as próteses parciais removíveis com macroapoios representam uma alternativa importante no tratamento de pacientes com redução da DVO e consequente restabelecimento das funções do sistema estomatognático, com aspectos conservadores e de reversibilidade.","PeriodicalId":10204,"journal":{"name":"Clinical and Laboratorial Research in Dentistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2020-03-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82022793","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-12-31DOI: 10.11606/ISSN.2357-8041.CLRD.2019.161294
M. T. Vasques, J. Mulder, D. Machado, D. Laganá
Objectives: The aim of this study is to compare the mechanical characteristics of resins for 3D printers with the acrylic resins that have been used in Dentistry, according to the post-processing method. Materials and Methods: Using an SLA 3D printer (Form 2 – Formlabs, Massachusetts, USA), samples (discs) were produced with the printer’s resins, Dental SG®, Dental LT®, Clear®, and Grey®. Samples made of thermopolimerized and auto polymerized acrylic resins were produced as well (Gold Standart parameters). The Knoop Hardness (KH) tests were performed using microdurometer HMV-2 (Shimadzu, Kyoto, Japan). Results: The results showed the Dental SG resin presented the highest KH, compared with thermopolimerized acrylic resin, the Clear and Dental LT resins KH were compared with auto polymerized acrylic resin, and the non-post cured Grey resin (not for intraoral use), as well as the Dental LT resin, showed the lowest KH. Conclusions: Considering the hardness, the printer’s resins are comparable with the acrylic resins established, when the post-processing method is thoroughly followed. The absence of material’s post-processing reduce significantly the material’s hardness. Clinical relevance: Regarding the use of 3D printing in Dentistry, the development of materials adequate for the equipment, biocompatible for intraoral uses, and compatible mechanical proprieties are essential.
{"title":"The influence of the post-processing method on knoop hardness of photosensitive resins for 3D SLA printer used in Dentistry","authors":"M. T. Vasques, J. Mulder, D. Machado, D. Laganá","doi":"10.11606/ISSN.2357-8041.CLRD.2019.161294","DOIUrl":"https://doi.org/10.11606/ISSN.2357-8041.CLRD.2019.161294","url":null,"abstract":"Objectives: The aim of this study is to compare the mechanical characteristics of resins for 3D printers with the acrylic resins that have been used in Dentistry, according to the post-processing method. Materials and Methods: Using an SLA 3D printer (Form 2 – Formlabs, Massachusetts, USA), samples (discs) were produced with the printer’s resins, Dental SG®, Dental LT®, Clear®, and Grey®. Samples made of thermopolimerized and auto polymerized acrylic resins were produced as well (Gold Standart parameters). The Knoop Hardness (KH) tests were performed using microdurometer HMV-2 (Shimadzu, Kyoto, Japan). Results: The results showed the Dental SG resin presented the highest KH, compared with thermopolimerized acrylic resin, the Clear and Dental LT resins KH were compared with auto polymerized acrylic resin, and the non-post cured Grey resin (not for intraoral use), as well as the Dental LT resin, showed the lowest KH. Conclusions: Considering the hardness, the printer’s resins are comparable with the acrylic resins established, when the post-processing method is thoroughly followed. The absence of material’s post-processing reduce significantly the material’s hardness. Clinical relevance: Regarding the use of 3D printing in Dentistry, the development of materials adequate for the equipment, biocompatible for intraoral uses, and compatible mechanical proprieties are essential.","PeriodicalId":10204,"journal":{"name":"Clinical and Laboratorial Research in Dentistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83590512","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-12-31DOI: 10.11606/ISSN.2357-8041.CLRD.2019.159943
Leonardo de Almeida, Bianca Medeiros Maran, Guilherme Schmitt de Andrade, Fabiana Scarparo Naufel, Vera Lúcia Schmitt
Smile, besides being an important factor in the self-esteem of human, has been considered the “ateway” to many opportunities today. However, there are some changes can be found and which can provide the aesthetic, functional and psychological impairment of the individual, like the diastema, defined by a spacing between adjacent teeth. Objectives: thus, we intend to show through a case an aesthetic rehabilitation of a young patient with the presence of diastema anterosuperiores teeth using composite resin of direct insertion and minimal wear of dental structure. Results: the result was extremely satisfying for both, the patient and the professional, proving the efficiency of composite resins in cases of rehabilitation when well indicated. Conclusion: thus, with the increase in the quality of technical and aesthetic materials, the use of this, subsequent to orthodontic treatment in patients who still show gaps between the teeth, provides ease and security to the professional in performing the procedure, obtaining better results. Smile, besides being an important factor in the self-esteem of human, has been considered the “ateway” to many opportunities today. However, there are some changes can be found and which can provide the aesthetic, functional and psychological impairment of the individual, like the diastema, defined by a spacing between adjacent teeth. Objectives: thus, we intend to show through a case an aesthetic rehabilitation of a young patient with the presence of diastema anterosuperiores teeth using composite resin of direct insertion and minimal wear of dental structure. Results: the result was extremely satisfying for both, the patient and the professional, proving the efficiency of composite resins in cases of rehabilitation when well indicated. Conclusion: thus, with the increase in the quality of technical and aesthetic materials, the use of this, subsequent to orthodontic treatment in patients who still show gaps between the teeth, provides ease and security to the professional in performing the procedure, obtaining better results.
{"title":"Reabilitação estética de diastemas anterossuperiores com resina composta após abordagem ortodôntica","authors":"Leonardo de Almeida, Bianca Medeiros Maran, Guilherme Schmitt de Andrade, Fabiana Scarparo Naufel, Vera Lúcia Schmitt","doi":"10.11606/ISSN.2357-8041.CLRD.2019.159943","DOIUrl":"https://doi.org/10.11606/ISSN.2357-8041.CLRD.2019.159943","url":null,"abstract":"Smile, besides being an important factor in the self-esteem of human, has been considered the “ateway” to many opportunities today. However, there are some changes can be found and which can provide the aesthetic, functional and psychological impairment of the individual, like the diastema, defined by a spacing between adjacent teeth. Objectives: thus, we intend to show through a case an aesthetic rehabilitation of a young patient with the presence of diastema anterosuperiores teeth using composite resin of direct insertion and minimal wear of dental structure. Results: the result was extremely satisfying for both, the patient and the professional, proving the efficiency of composite resins in cases of rehabilitation when well indicated. Conclusion: thus, with the increase in the quality of technical and aesthetic materials, the use of this, subsequent to orthodontic treatment in patients who still show gaps between the teeth, provides ease and security to the professional in performing the procedure, obtaining better results. \u0000Smile, besides being an important factor in the self-esteem of human, has been considered the “ateway” to many opportunities today. However, there are some changes can be found and which can provide the aesthetic, functional and psychological impairment of the individual, like the diastema, defined by a spacing between adjacent teeth. Objectives: thus, we intend to show through a case an aesthetic rehabilitation of a young patient with the presence of diastema anterosuperiores teeth using composite resin of direct insertion and minimal wear of dental structure. Results: the result was extremely satisfying for both, the patient and the professional, proving the efficiency of composite resins in cases of rehabilitation when well indicated. Conclusion: thus, with the increase in the quality of technical and aesthetic materials, the use of this, subsequent to orthodontic treatment in patients who still show gaps between the teeth, provides ease and security to the professional in performing the procedure, obtaining better results.","PeriodicalId":10204,"journal":{"name":"Clinical and Laboratorial Research in Dentistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84037779","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-12-27DOI: 10.11606/issn.2357-8041.clrd.2019.160296
V. Dhanvantri, R. Ramya, K. Cherian, Balasundari Ramesh
Objective: Nanotopography and soluble extracellular factors are present in the dental stem cell niche in the pulp. Their effect on dental stem cell survival and differentiation is yet to be established. We aimed to analyze the individual and combined roles of extracellular matrix (ECM) nanotopography and serum (soluble factors) on the growth, differentiation potential, and morphological characteristics of the human dental pulp stem cells (hDPSC). This study aimed to evaluate and compare the hDPSC response to different environmental cues – nanofibers, serum, and conditioned media. Materials and methods: In this study, fabricated PLLA nanofibers were used as the in vitro structural biomimetic of the native nanotopography found in the in vivo ECM/stem cell niche. Serum and conditioned media were used as the in vitro mimic of the soluble factors to which stem cells get exposed in vivo. hDPSC were grown in the presence and absence of biodegradablepoly-L-lactic-acid nanofibers and serum. The growth characteristics of hDPSC were assessed in terms of cell viability and doubling time at the interval of every passage. Cellular morphological changes were studied using inverted microscopy and H&E. As the second part of the study, hDPSC in all culture conditions were exposed to Dental Pulp Conditioned Media (DPCM) for a short duration of 3 days. After transient exposure to DPCM, the growth characteristics and the morphological changes of hDPSC were assessed. In addition, scanning electron microscopy was used for the morphological study of hDPSC on nanofibers, exposed to conditioned media. The differentiated cells were analyzed by qRT-PCR for neurogenic and odontogenic expression of RUNX2, osteopontin, and β-tubulin III genes. Results: hDPSC showed better survival and proliferation in the presence of nanofibers and serum. Absence of nanofibers or serum greatly altered stem cell survival and proliferation and also indicated differentiation. In addition, it was observed that after transient exposure to DPCM, the presence of both PLLA nanofiber and serum favoured higher odontogenic and neurogenic differentiation potential, without characteristic morphological changes of terminal differentiation. Conclusion: hDPSC has the ability to sense nanoscale geometric cues from their microenvironment. Nanotopography and soluble factors of the extracellular matrix both affect hDPSC. Further studies are essential to identify the key pathways that play a vital role in such interactions. The hDPSC demonstrated better survival and proliferation in the presence of nanofibers and serum. Absence of nanofibers or serum greatly altered stem cell survival and proliferation and also showed changes indicative of differentiation. The results were compared and analyzed using GraphPad Prism 5 Software. hDPSC possess the ability to sense nanoscale geometric cues from their microenvironment. Nanotopography and soluble factors of the Extracellular matrix together influence the fate of hDPSC.
{"title":"Evaluation of human dental stem cell growth characteristics and cellular morphological changes in response to extracellular matrix nanotopography","authors":"V. Dhanvantri, R. Ramya, K. Cherian, Balasundari Ramesh","doi":"10.11606/issn.2357-8041.clrd.2019.160296","DOIUrl":"https://doi.org/10.11606/issn.2357-8041.clrd.2019.160296","url":null,"abstract":"Objective: Nanotopography and soluble extracellular factors are present in the dental stem cell niche in the pulp. Their effect on dental stem cell survival and differentiation is yet to be established. We aimed to analyze the individual and combined roles of extracellular matrix (ECM) nanotopography and serum (soluble factors) on the growth, differentiation potential, and morphological characteristics of the human dental pulp stem cells (hDPSC). This study aimed to evaluate and compare the hDPSC response to different environmental cues – nanofibers, serum, and conditioned media. Materials and methods: In this study, fabricated PLLA nanofibers were used as the in vitro structural biomimetic of the native nanotopography found in the in vivo ECM/stem cell niche. Serum and conditioned media were used as the in vitro mimic of the soluble factors to which stem cells get exposed in vivo. hDPSC were grown in the presence and absence of biodegradablepoly-L-lactic-acid nanofibers and serum. The growth characteristics of hDPSC were assessed in terms of cell viability and doubling time at the interval of every passage. Cellular morphological changes were studied using inverted microscopy and H&E. As the second part of the study, hDPSC in all culture conditions were exposed to Dental Pulp Conditioned Media (DPCM) for a short duration of 3 days. After transient exposure to DPCM, the growth characteristics and the morphological changes of hDPSC were assessed. In addition, scanning electron microscopy was used for the morphological study of hDPSC on nanofibers, exposed to conditioned media. The differentiated cells were analyzed by qRT-PCR for neurogenic and odontogenic expression of RUNX2, osteopontin, and β-tubulin III genes. Results: hDPSC showed better survival and proliferation in the presence of nanofibers and serum. Absence of nanofibers or serum greatly altered stem cell survival and proliferation and also indicated differentiation. In addition, it was observed that after transient exposure to DPCM, the presence of both PLLA nanofiber and serum favoured higher odontogenic and neurogenic differentiation potential, without characteristic morphological changes of terminal differentiation. Conclusion: hDPSC has the ability to sense nanoscale geometric cues from their microenvironment. Nanotopography and soluble factors of the extracellular matrix both affect hDPSC. Further studies are essential to identify the key pathways that play a vital role in such interactions. The hDPSC demonstrated better survival and proliferation in the presence of nanofibers and serum. Absence of nanofibers or serum greatly altered stem cell survival and proliferation and also showed changes indicative of differentiation. The results were compared and analyzed using GraphPad Prism 5 Software. hDPSC possess the ability to sense nanoscale geometric cues from their microenvironment. Nanotopography and soluble factors of the Extracellular matrix together influence the fate of hDPSC. ","PeriodicalId":10204,"journal":{"name":"Clinical and Laboratorial Research in Dentistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-12-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77795490","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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