Pub Date : 2019-12-06DOI: 10.11606/issn.2357-8041.clrd.2019.157790
Helidior Teixeira Lopes, L. Alves, Alexandra Flávia Gazzoni
To evaluate how the addition of 0.5% silica nanoparticles and/or photopolymerizable coatings affect the adhesion of Candida albicans on the surface of acrylic resin PMMA (poly-methyl methacrylate). Materials and methods: A total of 40 specimens were fabricated (65 mm × 10 mm × 2,5 mm) and randomly divided into four experimental groups according to surface treatment using silica and/or photopolymerizable coating: the control group (G1); silica group (G2) (0.5% silica); photopolymerizable coating group (G3); and silica group (0.5% silica) + photopolymerizable coatings (G4). Specimens were separately incubated with a Candida albicans suspension spectrophotometrically standardized with 1 × 107 CFU/ml at 37 °C for 90 minutes. After this period, the specimens were removed and then their fungal adhesion was analyzed by the colonyforming units counting. Data were analyzed by the One-way ANOVA test followed by the Tukey test (P ≤ 0,05). Results: A significant reduction in Candida albicans adhesion was observed in G2, G3, and G4 when compared to the control group G1 (P ≤ 0.0001). However, similar fungal adhesion was observed when G2, G3, and G4 were compared (P ≤ 0.05). Conclusions: The addition of silica nanoparticles and/or photopolymerizable coating on the surface of PMMA decreases the adhesion of Candida albicans, which can be used as a strategy to reduce oral lesions caused by acrylic dentures.
评估添加0.5%二氧化硅纳米颗粒和/或光聚合涂层如何影响白色念珠菌在丙烯酸树脂PMMA(聚甲基丙烯酸甲酯)表面的粘附。材料与方法:共制作40个样品(65 mm × 10 mm × 2.5 mm)，根据表面处理二氧化硅和/或光聚合涂层的不同，随机分为4个实验组:对照组(G1);二氧化硅组(G2)(0.5%二氧化硅);光聚合涂层组(G3);二氧化硅基团(0.5%二氧化硅)+光聚合涂层(G4)。标本分别与1 × 107 CFU/ml分光光度标准化的白色念珠菌悬液在37℃下孵育90分钟。这段时间后，取出标本，通过菌落形成单位计数分析其真菌粘附性。数据分析采用单因素方差分析和Tukey检验(P≤0.05)。结果:与对照组G1相比，G2、G3、G4组白色念珠菌粘连明显降低(P≤0.0001)。G2、G3、G4组真菌粘附情况相似(P≤0.05)。结论:在PMMA表面添加二氧化硅纳米颗粒和/或光聚合涂层可减少白色念珠菌的粘附，可作为减少丙烯酸义齿口腔病变的策略。
{"title":"Avaliação da adesão de Candida albicans na superfície de resinas acrílicas termopolimerizáveis tratadas com glaze fotopolimerizável e/ou adição de nanopartículas de sílica","authors":"Helidior Teixeira Lopes, L. Alves, Alexandra Flávia Gazzoni","doi":"10.11606/issn.2357-8041.clrd.2019.157790","DOIUrl":"https://doi.org/10.11606/issn.2357-8041.clrd.2019.157790","url":null,"abstract":"To evaluate how the addition of 0.5% silica nanoparticles and/or photopolymerizable coatings affect the adhesion of Candida albicans on the surface of acrylic resin PMMA (poly-methyl methacrylate). Materials and methods: A total of 40 specimens were fabricated (65 mm × 10 mm × 2,5 mm) and randomly divided into four experimental groups according to surface treatment using silica and/or photopolymerizable coating: the control group (G1); silica group (G2) (0.5% silica); photopolymerizable coating group (G3); and silica group (0.5% silica) + photopolymerizable coatings (G4). Specimens were separately incubated with a Candida albicans suspension spectrophotometrically standardized with 1 × 107 CFU/ml at 37 °C for 90 minutes. After this period, the specimens were removed and then their fungal adhesion was analyzed by the colonyforming units counting. Data were analyzed by the One-way ANOVA test followed by the Tukey test (P ≤ 0,05). Results: A significant reduction in Candida albicans adhesion was observed in G2, G3, and G4 when compared to the control group G1 (P ≤ 0.0001). However, similar fungal adhesion was observed when G2, G3, and G4 were compared (P ≤ 0.05). Conclusions: The addition of silica nanoparticles and/or photopolymerizable coating on the surface of PMMA decreases the adhesion of Candida albicans, which can be used as a strategy to reduce oral lesions caused by acrylic dentures.","PeriodicalId":10204,"journal":{"name":"Clinical and Laboratorial Research in Dentistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-12-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75919008","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-12-04DOI: 10.11606/issn.2357-8041.clrd.2019.157759
Wladimir Gushiken de Campos, Camilla Vieira Esteves, K. Costa, A. C. Andrade, C. Domaneschi, C. Lemos
| Saliva is important for the preservation and maintenance of normal physiological conditions of oral tissues, and factors influencing its decrease are associated with the risk for oral diseases. Xerostomia is the subjective sensation of oral dryness, which can be influenced by etiological factors including the use of several types of medications, Sjogren’s and metabolic syndromes, head and neck irradiation, among others. Treatment methods for xerostomia vary and can be local or systemic. The aim of this article is to present the results of a literature review addressing the correlation between xerostomia and its incidence and prevalence in the older adult population. Results of this review highlight the importance of diagnosis and appropriate management, and briefly describe treatment modalities and etiological factors such as medications, Sjogren’s syndrome, metabolic syndrome, and head and neck irradiation. DESCRIPTORS | Xerostomia; Dry mouth; Saliva. RESUMO | Xerostomia na população idosa, do diagnóstico ao tratamento: revisão da literatura • A saliva é importante para a preservação e manutenção das condições fisiológicas normais dos tecidos bucais, e os fatores que influenciam sua diminuição estão associados ao risco de doenças bucais. Xerostomia é a sensação subjetiva de secura oral, que pode ser influenciada por fatores etiológicos, incluindo o uso de vários tipos de medicamentos, síndromes metabólicas e de Sjögren, irradiação de cabeça e pescoço, entre outros. Os métodos de tratamento para xerostomia variam e podem ser locais ou sistêmicos. O objetivo deste artigo é apresentar os resultados de uma revisão bibliográfica abordando a correlação entre xerostomia e sua incidência e prevalência na população idosa. Os resultados desta revisão destacam a importância do diagnóstico e do manejo adequado e descrevem brevemente as modalidades de tratamento e fatores etiológicos, como medicamentos, síndrome de Sjögren, síndrome metabólica e irradiação de cabeça e pescoço. DESCRITORES | Xerostomia; Boca seca; Saliva. CORRESPONDING AUTHOR | • Wladimir Gushiken de Campos Department of Oral Medicine, Faculdade de Odontologia, Universidade de São Paulo • Av. Prof. Lineu Prestes, 2227 • São Paulo, SP, Brazil • 05508-00 • E-mail: wgushiken@hotmail.com • Received May 06, 2019. • Accepted Aug. 05, 2019. • DOI http://dx.doi.org/10.11606/issn.2357-8041.clrd.2019.157759 Xerostomia in the older adult population, from diagnosis to treatment: a literature review 2 ● Clin Lab Res Den 2019: 1-7 INTRODUCTION Xerostomia is characterized as the subjective sensation of dry mouth, whereas hyposalivation describes a reduction in salivary flow. Xerostomia, however, does not necessarily imply in a decrease in salivary flow because patients who do not exhibit hyposalivation may exhibit xerostomia.1 Salivary function remains remarkably intact in healthy older adults; however, numerous etiological factors can induce and/or maintain xerostomia, including dehydration, mouth bre
干燥的粘膜和吞咽困难的表面用口服保湿剂和润滑剂、人工唾液和
{"title":"Xerostomia in the older adult population, from diagnosis to treatment","authors":"Wladimir Gushiken de Campos, Camilla Vieira Esteves, K. Costa, A. C. Andrade, C. Domaneschi, C. Lemos","doi":"10.11606/issn.2357-8041.clrd.2019.157759","DOIUrl":"https://doi.org/10.11606/issn.2357-8041.clrd.2019.157759","url":null,"abstract":"| Saliva is important for the preservation and maintenance of normal physiological conditions of oral tissues, and factors influencing its decrease are associated with the risk for oral diseases. Xerostomia is the subjective sensation of oral dryness, which can be influenced by etiological factors including the use of several types of medications, Sjogren’s and metabolic syndromes, head and neck irradiation, among others. Treatment methods for xerostomia vary and can be local or systemic. The aim of this article is to present the results of a literature review addressing the correlation between xerostomia and its incidence and prevalence in the older adult population. Results of this review highlight the importance of diagnosis and appropriate management, and briefly describe treatment modalities and etiological factors such as medications, Sjogren’s syndrome, metabolic syndrome, and head and neck irradiation. DESCRIPTORS | Xerostomia; Dry mouth; Saliva. RESUMO | Xerostomia na população idosa, do diagnóstico ao tratamento: revisão da literatura • A saliva é importante para a preservação e manutenção das condições fisiológicas normais dos tecidos bucais, e os fatores que influenciam sua diminuição estão associados ao risco de doenças bucais. Xerostomia é a sensação subjetiva de secura oral, que pode ser influenciada por fatores etiológicos, incluindo o uso de vários tipos de medicamentos, síndromes metabólicas e de Sjögren, irradiação de cabeça e pescoço, entre outros. Os métodos de tratamento para xerostomia variam e podem ser locais ou sistêmicos. O objetivo deste artigo é apresentar os resultados de uma revisão bibliográfica abordando a correlação entre xerostomia e sua incidência e prevalência na população idosa. Os resultados desta revisão destacam a importância do diagnóstico e do manejo adequado e descrevem brevemente as modalidades de tratamento e fatores etiológicos, como medicamentos, síndrome de Sjögren, síndrome metabólica e irradiação de cabeça e pescoço. DESCRITORES | Xerostomia; Boca seca; Saliva. CORRESPONDING AUTHOR | • Wladimir Gushiken de Campos Department of Oral Medicine, Faculdade de Odontologia, Universidade de São Paulo • Av. Prof. Lineu Prestes, 2227 • São Paulo, SP, Brazil • 05508-00 • E-mail: wgushiken@hotmail.com • Received May 06, 2019. • Accepted Aug. 05, 2019. • DOI http://dx.doi.org/10.11606/issn.2357-8041.clrd.2019.157759 Xerostomia in the older adult population, from diagnosis to treatment: a literature review 2 ● Clin Lab Res Den 2019: 1-7 INTRODUCTION Xerostomia is characterized as the subjective sensation of dry mouth, whereas hyposalivation describes a reduction in salivary flow. Xerostomia, however, does not necessarily imply in a decrease in salivary flow because patients who do not exhibit hyposalivation may exhibit xerostomia.1 Salivary function remains remarkably intact in healthy older adults; however, numerous etiological factors can induce and/or maintain xerostomia, including dehydration, mouth bre","PeriodicalId":10204,"journal":{"name":"Clinical and Laboratorial Research in Dentistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-12-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81074294","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-10-22DOI: 10.11606/issn.2357-8041.clrd.2019.160071
F. C. Salineiro, Solange Kobayashi-Velasco, I. P. Tortamano, R. G. Rocha, M. Cavalcanti
Objective: This study proposed to locate the mandibular foramen position by using cone beam computed tomography (CBCT) imaging, based on landmarks used at inferior alveolar nerve block (IANB) technique in order to increase the success rate of this procedure. Materials and methods: 58 mandibular foramina of 29 adult patients were evaluated. Linear and angular CBCT measurements were defined for the mandibular foramen based on the IANB technique. The measurements were performed by two radiologists. Data analyses consisted of verifying the normality, obtaining the mean value of all patients and comparing the data obtained between male and female patients. Results: Student’s t and Mann-Whitney’s U tests were used to calculate the mean mandibular foramen position. The three mean position measurements of the mandibular foramen were 13.5 mm above the occlusal plan (15.5 mm for male and 11.7 mm for female patients), 14.6 mm below the mandibular notch (14.9 mm for male and 13.7 mm for female patients) and 14.2 mm from the anterior border of mandibular ramus (14.6 mm for male and 13.8 mm for female patients). The angle formed by the molar lines and contralateral premolar lines was 25.8° and the angle formed by the molar lines and contralateral second premolar/molar lines was 31.5°. Conclusion: CBCT images were a useful method to provide the measurements of the mandibular foramen position based on landmarks used by the IANB technique. We encourage the use of these measurements as reference to increase the clinical success when performing the IANB technique.
{"title":"Mandibular foramen location","authors":"F. C. Salineiro, Solange Kobayashi-Velasco, I. P. Tortamano, R. G. Rocha, M. Cavalcanti","doi":"10.11606/issn.2357-8041.clrd.2019.160071","DOIUrl":"https://doi.org/10.11606/issn.2357-8041.clrd.2019.160071","url":null,"abstract":"Objective: This study proposed to locate the mandibular foramen position by using cone beam computed tomography (CBCT) imaging, based on landmarks used at inferior alveolar nerve block (IANB) technique in order to increase the success rate of this procedure. Materials and methods: 58 mandibular foramina of 29 adult patients were evaluated. Linear and angular CBCT measurements were defined for the mandibular foramen based on the IANB technique. The measurements were performed by two radiologists. Data analyses consisted of verifying the normality, obtaining the mean value of all patients and comparing the data obtained between male and female patients. Results: Student’s t and Mann-Whitney’s U tests were used to calculate the mean mandibular foramen position. The three mean position measurements of the mandibular foramen were 13.5 mm above the occlusal plan (15.5 mm for male and 11.7 mm for female patients), 14.6 mm below the mandibular notch (14.9 mm for male and 13.7 mm for female patients) and 14.2 mm from the anterior border of mandibular ramus (14.6 mm for male and 13.8 mm for female patients). The angle formed by the molar lines and contralateral premolar lines was 25.8° and the angle formed by the molar lines and contralateral second premolar/molar lines was 31.5°. Conclusion: CBCT images were a useful method to provide the measurements of the mandibular foramen position based on landmarks used by the IANB technique. We encourage the use of these measurements as reference to increase the clinical success when performing the IANB technique.","PeriodicalId":10204,"journal":{"name":"Clinical and Laboratorial Research in Dentistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-10-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78628758","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-10-15DOI: 10.11606/issn.2357-8041.clrd.2019.158306
D. Haddad, B. Oliveira, M. Brioschi, E. Crosato, R. Vardasca, J. Mendes, J. Pinho, M. Clemente, E. Arita
Infrared thermography is a functional examination that can document physiological changes such as abnormal cutaneous vasomotor activity in inflammatory and neurogenic pictures related to nociceptive and neuropathic pain by mapping the thermal distribution on the surface of the skin. Objective: The aim of this study was to verify if there is a facial thermal difference between the symptomatic and asymptomatic group for myogenic TMD according to the Research Diagnostic Criteria for Temporomandibular Disorder (RDC/TMD) in a European population sample. Material and methods: Sixty-one subjects between 20 and 40 years (26.2 ± 7.6 years) of both sexes were divided into two groups. The 28 facial thermo-anatomic points were selected and the values of minimum (Tmin), mean (Tmed) and maximum (Tmax) temperatures, average of hemiface temperatures of whole sample, temperature difference (ΔT(°C)) between groups and from these data an algorithm was formulated to separate the groups with greater accuracy. Results: There was an average difference of 0.3 °C of all points when comparing the two groups. The symptomatic group had lower maximum temperature for frontal and lateral views when compared to the asymptomatic group (p<0.05), and presented lower average temperature in frontal view (p<0.05). Symptomatic individuals for myogenic TMD presented a reduction of facial cutaneous blood flow corresponding to lower maximum temperature by the proposed method of analysis of thermal anatomical points. Conclusions: Infrared thermography showed potential to be a screening and complementary diagnostic examination method for patients with myogenic temporomandibular disorders in the daily clinic just by frontal face image.
{"title":"Is it possible myogenic temporomandibular dysfunctions change the facial thermal imaging?","authors":"D. Haddad, B. Oliveira, M. Brioschi, E. Crosato, R. Vardasca, J. Mendes, J. Pinho, M. Clemente, E. Arita","doi":"10.11606/issn.2357-8041.clrd.2019.158306","DOIUrl":"https://doi.org/10.11606/issn.2357-8041.clrd.2019.158306","url":null,"abstract":"Infrared thermography is a functional examination that can document physiological changes such as abnormal cutaneous vasomotor activity in inflammatory and neurogenic pictures related to nociceptive and neuropathic pain by mapping the thermal distribution on the surface of the skin. Objective: The aim of this study was to verify if there is a facial thermal difference between the symptomatic and asymptomatic group for myogenic TMD according to the Research Diagnostic Criteria for Temporomandibular Disorder (RDC/TMD) in a European population sample. Material and methods: Sixty-one subjects between 20 and 40 years (26.2 ± 7.6 years) of both sexes were divided into two groups. The 28 facial thermo-anatomic points were selected and the values of minimum (Tmin), mean (Tmed) and maximum (Tmax) temperatures, average of hemiface temperatures of whole sample, temperature difference (ΔT(°C)) between groups and from these data an algorithm was formulated to separate the groups with greater accuracy. Results: There was an average difference of 0.3 °C of all points when comparing the two groups. The symptomatic group had lower maximum temperature for frontal and lateral views when compared to the asymptomatic group (p<0.05), and presented lower average temperature in frontal view (p<0.05). Symptomatic individuals for myogenic TMD presented a reduction of facial cutaneous blood flow corresponding to lower maximum temperature by the proposed method of analysis of thermal anatomical points. Conclusions: Infrared thermography showed potential to be a screening and complementary diagnostic examination method for patients with myogenic temporomandibular disorders in the daily clinic just by frontal face image.","PeriodicalId":10204,"journal":{"name":"Clinical and Laboratorial Research in Dentistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88381873","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-10-09DOI: 10.11606/issn.2357-8041.clrd.2019.157807
I. G. G. Choi, L. Munhoz, Erika Miti Yasui, C. Iida, Natalia Isis Caires Lavor, E. Arita
Background: Peripheral Dual-energy X-ray absorptiometry (DXA) can be applied to identify low bone mineral density (BMD) patients, however, DXA is not sufficiently available in many countries. Thus, the use of simpler and cheaper screening tools than DXA to detect low BMD become indispensable. Objectives: The objective of this study was to correlate São Paulo osteoporosis risk index (Sapori) and mandibular cortical index (MCI) screening tools with the femur DXA results of patients in our sample of Brazilian women. A secondary objective was to assess which of these screening tools better correlates with the results of the Frax tool. Material and Methods: Exams of women who had undergone panoramic radiographic examination at the beginning of dental treatment and peripheral DXA for screening osteoporosis from 2010 to 2014 were included. The MCI was evaluated, and Sapori and Frax were calculated with their available online software tools. Spearman correlation was performed to analyse the level of correlation between femur T-scores (and MCI and Sapori values, as well to perform the level of correlation between Frax and MCI and Sapori values. Results: The spearman correlation comparing the numerical T-score values and the categorical values of MCI (rs=-0.274), and of Sapori (rs=-0.470), showed a statistically significant inverse correlation for both equations (p<0.01). The Spearman equation comparing the values obtained with Frax and MCI did not show a statistically signifcant correlation (p>0.01). But the equation comparing the values obtained with Frax and Sapori showed a positive, moderate and statistically significant correlation between them (rs=0.460). Conclusion: MCI is not a reliable screening tool to identify women with low BMD or Osteoporosis, whereas more optimistic results were observed for the Sapori.
{"title":"Correlation between MCI/Sapori screening tools and DXA results and between MCI/Sapori and Frax tool for estimating fracture risk","authors":"I. G. G. Choi, L. Munhoz, Erika Miti Yasui, C. Iida, Natalia Isis Caires Lavor, E. Arita","doi":"10.11606/issn.2357-8041.clrd.2019.157807","DOIUrl":"https://doi.org/10.11606/issn.2357-8041.clrd.2019.157807","url":null,"abstract":"Background: Peripheral Dual-energy X-ray absorptiometry (DXA) can be applied to identify low bone mineral density (BMD) patients, however, DXA is not sufficiently available in many countries. Thus, the use of simpler and cheaper screening tools than DXA to detect low BMD become indispensable. Objectives: The objective of this study was to correlate São Paulo osteoporosis risk index (Sapori) and mandibular cortical index (MCI) screening tools with the femur DXA results of patients in our sample of Brazilian women. A secondary objective was to assess which of these screening tools better correlates with the results of the Frax tool. Material and Methods: Exams of women who had undergone panoramic radiographic examination at the beginning of dental treatment and peripheral DXA for screening osteoporosis from 2010 to 2014 were included. The MCI was evaluated, and Sapori and Frax were calculated with their available online software tools. Spearman correlation was performed to analyse the level of correlation between femur T-scores (and MCI and Sapori values, as well to perform the level of correlation between Frax and MCI and Sapori values. Results: The spearman correlation comparing the numerical T-score values and the categorical values of MCI (rs=-0.274), and of Sapori (rs=-0.470), showed a statistically significant inverse correlation for both equations (p<0.01). The Spearman equation comparing the values obtained with Frax and MCI did not show a statistically signifcant correlation (p>0.01). But the equation comparing the values obtained with Frax and Sapori showed a positive, moderate and statistically significant correlation between them (rs=0.460). Conclusion: MCI is not a reliable screening tool to identify women with low BMD or Osteoporosis, whereas more optimistic results were observed for the Sapori.","PeriodicalId":10204,"journal":{"name":"Clinical and Laboratorial Research in Dentistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87656404","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-08-27DOI: 10.11606/issn.2357-8041.clrd.2019.151831
M. Brozoski, Natalia Caroline Aguiar Tartaroti, Andreia Aparecida Trainá, M. Deboni, M. Marques, Maria da Graça Naclério Homem
The goal of this study is to evaluate the effect of photobiomodulation therapy (PBMT) on the viability of osteoblasts and cultured fibroblasts in different concentrations of alendronate or zoledronic acid. Two cell lines: osteoblast-like mouse cells (OSTEO 1) and human buccal mucosa fibroblast (FMM1) were used. Cells were submitted to different concentrations of bisphosphonates (1 μM, 10 μM, and 100 μM sodium alendronate and 3 μM, 5 μM and 10 μM zoledronic acid) for 24 hours. Next, the cultures received PBMT. The irradiations were applied with a diode laser (InGaAIP, 660 nm, 30 mW, spot 0.028 cm2) in continuous, punctual and contact mode at two energy densities: 5 J/cm2 (4.5 s) or 10 Jcm2 (9s) with 6 hours-intervals. Cell viability was determined by mitochondrial activity assay (MTT) 24 h after the last irradiation. The data were compared by the one way- ANOVA, complemented by the Tukey’s test (p < 0.05). Sodium alendronate at concentrations of 100 μM and 10 μM and zoledronic acid at 10 μM concentration showed higher long-term toxicity. The cellular viability of the PBMT treated group was significantly higher than that of the negative control group. The same occurred with the osteoblasts treated with the highest concentrations of the drug (5 and 10 μM), despite not reaching the cell viability of the positive control group, it presented greater viability than the negative control where the cells were not irradiated. In the groups submitted to zoledronic acid, positive controls presented greater cell viability. We concluded that under the parameters applied in this study, PBMT at an energy density of 5 J/cm2 was able to revert the toxicity of sodium alendronate applied at the higher concentrations in both cell types, whereas zoledronic acid toxicity, regardless of its concentrations, was not influenced by PBMT.
{"title":"Effect of photobiomodulation on the viability of osteoblasts and fibroblasts submitted to alendronate sodium or zoledronic acid","authors":"M. Brozoski, Natalia Caroline Aguiar Tartaroti, Andreia Aparecida Trainá, M. Deboni, M. Marques, Maria da Graça Naclério Homem","doi":"10.11606/issn.2357-8041.clrd.2019.151831","DOIUrl":"https://doi.org/10.11606/issn.2357-8041.clrd.2019.151831","url":null,"abstract":"The goal of this study is to evaluate the effect of photobiomodulation therapy (PBMT) on the viability of osteoblasts and cultured fibroblasts in different concentrations of alendronate or zoledronic acid. Two cell lines: osteoblast-like mouse cells (OSTEO 1) and human buccal mucosa fibroblast (FMM1) were used. Cells were submitted to different concentrations of bisphosphonates (1 μM, 10 μM, and 100 μM sodium alendronate and 3 μM, 5 μM and 10 μM zoledronic acid) for 24 hours. Next, the cultures received PBMT. The irradiations were applied with a diode laser (InGaAIP, 660 nm, 30 mW, spot 0.028 cm2) in continuous, punctual and contact mode at two energy densities: 5 J/cm2 (4.5 s) or 10 Jcm2 (9s) with 6 hours-intervals. Cell viability was determined by mitochondrial activity assay (MTT) 24 h after the last irradiation. The data were compared by the one way- ANOVA, complemented by the Tukey’s test (p < 0.05). Sodium alendronate at concentrations of 100 μM and 10 μM and zoledronic acid at 10 μM concentration showed higher long-term toxicity. The cellular viability of the PBMT treated group was significantly higher than that of the negative control group. The same occurred with the osteoblasts treated with the highest concentrations of the drug (5 and 10 μM), despite not reaching the cell viability of the positive control group, it presented greater viability than the negative control where the cells were not irradiated. In the groups submitted to zoledronic acid, positive controls presented greater cell viability. We concluded that under the parameters applied in this study, PBMT at an energy density of 5 J/cm2 was able to revert the toxicity of sodium alendronate applied at the higher concentrations in both cell types, whereas zoledronic acid toxicity, regardless of its concentrations, was not influenced by PBMT.","PeriodicalId":10204,"journal":{"name":"Clinical and Laboratorial Research in Dentistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-08-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80271211","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-07-17DOI: 10.11606/ISSN.2357-8041.CLRD.2019.153387
João Felipe Besegato, Isabela Alves Matioli, Adrieli Burey, D. Poletto, A. O. Silva, Márcio Grama Hoeppner
Objetivo: Relatar e discutir o tratamento realizado para solucionar o problema estético decorrente da alteração cromática do incisivo central superior esquerdo (21), tratado endodonticamente e com restauração insatisfatória de resina composta. Relato do caso: Agentes clareadores à base de perborato de sódio e/ou peróxido de hidrogênio foram utilizados em técnicas intra e extracoronária, imediata e mediata. Após as sessões de clareamento dentário, o resultado obtido não foi satisfatório frente aos anseios do paciente, fato que justificou a substituição da restauração em resina composta com recobrimento da face vestibular (faceta direta). Conclusão: Considerando os resultados clínicos obtidos, pôde-se concluir que o clareamento dentário, embora conservador às estruturas dentárias, não foi efetivo à resolução do problema decorrente da alteração cromática. Por sua vez, o desgaste da face vestibular e posterior restauração direta com resina composta, embora menos conservador, foi satisfatório para solucionar o problema estético e se mostra efetivo após dois anos de controle clínico.
{"title":"Associação de procedimentos para a resolução estética de dente com alteração de cor e tratado endodonticamente","authors":"João Felipe Besegato, Isabela Alves Matioli, Adrieli Burey, D. Poletto, A. O. Silva, Márcio Grama Hoeppner","doi":"10.11606/ISSN.2357-8041.CLRD.2019.153387","DOIUrl":"https://doi.org/10.11606/ISSN.2357-8041.CLRD.2019.153387","url":null,"abstract":"Objetivo: Relatar e discutir o tratamento realizado para solucionar o problema estético decorrente da alteração cromática do incisivo central superior esquerdo (21), tratado endodonticamente e com restauração insatisfatória de resina composta. Relato do caso: Agentes clareadores à base de perborato de sódio e/ou peróxido de hidrogênio foram utilizados em técnicas intra e extracoronária, imediata e mediata. Após as sessões de clareamento dentário, o resultado obtido não foi satisfatório frente aos anseios do paciente, fato que justificou a substituição da restauração em resina composta com recobrimento da face vestibular (faceta direta). Conclusão: Considerando os resultados clínicos obtidos, pôde-se concluir que o clareamento dentário, embora conservador às estruturas dentárias, não foi efetivo à resolução do problema decorrente da alteração cromática. Por sua vez, o desgaste da face vestibular e posterior restauração direta com resina composta, embora menos conservador, foi satisfatório para solucionar o problema estético e se mostra efetivo após dois anos de controle clínico.","PeriodicalId":10204,"journal":{"name":"Clinical and Laboratorial Research in Dentistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-07-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72972808","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-07-03DOI: 10.11606/ISSN.2357-8041.CLRD.2019.154818
Marjorie Fonseca da Cunha, Francine Kulh Panzarella, Gonzalo André Montesinos, Juliane Pirágine Araujo, Ricardo Raitz
Objetivos: Avaliar a interferência de artefatos (beamhardening) na detecção de fratura radicular vertical por meio da tomografia computadorizada de feixe cônico, bem como a sensibilidade, a especificidade e a acurácia. Materiais e métodos: Foram utilizadas 15 raízes de incisivos inferiores bovinos que, após a realização do tratamento endodôntico e desobturação de 10 mm, foram reabilitadas com núcleo metálico fundido, cuja cimentação foi efetuada com cimento de fosfato de zinco. Os exames de tomografia computadorizada de feixe cônico foram realizados antes (T0) e após (T1) a indução das fraturas, as quais foram avaliadas por dois examinadores, a fim de identificar a presença e a localização das fraturas radiculares, bem como a localização do beamhardening e sua interferência na detecção das fraturas radiculares verticais, utilizando o filtro Angio_Sharpen High 5X5. Resultados: O beamhardening foi observado em 100% das raízes e foi visualizado com mais frequência no terço radicular médio. Em ambos os momentos de avaliação (T0 e T1), os locais de beamhardening eram proporcionalmente os mesmos que os locais das fraturas, gerando diagnósticos positivos de fratura mesmo quando elas não existiam. Conclusões: A tomografia computadorizada de feixe cônico apresentou 78% de sensibilidade, 63% de especificidade e 68% de acurácia, demonstrando ser apenas um método auxiliar no diagnóstico de fraturas radiculares em dentes reabilitados com núcleo metálico fundido.
{"title":"Interferência de artefatos na detecção de fraturas radiculares verticais em dentes bovinos com núcleo metálico fundido em imagens tomográficas de feixe cônico","authors":"Marjorie Fonseca da Cunha, Francine Kulh Panzarella, Gonzalo André Montesinos, Juliane Pirágine Araujo, Ricardo Raitz","doi":"10.11606/ISSN.2357-8041.CLRD.2019.154818","DOIUrl":"https://doi.org/10.11606/ISSN.2357-8041.CLRD.2019.154818","url":null,"abstract":"Objetivos: Avaliar a interferência de artefatos (beamhardening) na detecção de fratura radicular vertical por meio da tomografia computadorizada de feixe cônico, bem como a sensibilidade, a especificidade e a acurácia. Materiais e métodos: Foram utilizadas 15 raízes de incisivos inferiores bovinos que, após a realização do tratamento endodôntico e desobturação de 10 mm, foram reabilitadas com núcleo metálico fundido, cuja cimentação foi efetuada com cimento de fosfato de zinco. Os exames de tomografia computadorizada de feixe cônico foram realizados antes (T0) e após (T1) a indução das fraturas, as quais foram avaliadas por dois examinadores, a fim de identificar a presença e a localização das fraturas radiculares, bem como a localização do beamhardening e sua interferência na detecção das fraturas radiculares verticais, utilizando o filtro Angio_Sharpen High 5X5. Resultados: O beamhardening foi observado em 100% das raízes e foi visualizado com mais frequência no terço radicular médio. Em ambos os momentos de avaliação (T0 e T1), os locais de beamhardening eram proporcionalmente os mesmos que os locais das fraturas, gerando diagnósticos positivos de fratura mesmo quando elas não existiam. Conclusões: A tomografia computadorizada de feixe cônico apresentou 78% de sensibilidade, 63% de especificidade e 68% de acurácia, demonstrando ser apenas um método auxiliar no diagnóstico de fraturas radiculares em dentes reabilitados com núcleo metálico fundido.","PeriodicalId":10204,"journal":{"name":"Clinical and Laboratorial Research in Dentistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88843078","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-06-26DOI: 10.11606/ISSN.2357-8041.CLRD.2019.157692
Fernanda Cristina Nogueira Rodrigues, Júlia Silva Gomes de Araújo, Erika Maria Santos de Araújo, Andrea Dias Neves Lago
Objetivo: Relatar um caso clínico utilizando a terapia de fotobiomodulação no tratamento da disfunção temporomandibular proporcionando analgesia e maior abertura bucal. Relato de caso: Paciente, gênero feminino, 24 anos e portadora de disfunção temporomandibular, foi atendida para tratamento no projeto de extensão “Laserterapia em Odontologia” da Universidade Federal do Maranhão. O diagnóstico foi feito através de uma anamnese criteriosa, exame clínico e aplicação de questionário. O aparelho de laser utilizado no tratamento foi o de baixa potência cujo meio ativo é composto por um diodo semicondutor de Arseneto de Gálio e Alumínio (GaAlAs). O protocolo utilizado para o tratamento foi de comprimento de onda 808 nm, 60 s, 6 J de energia por ponto, em um total de dez sessões. Já na segunda sessão, durante a avaliação, a paciente relatou melhora significativa do grau de dor e a medida de abertura bucal variou de 25 mm a 40 mm. Conclusão: A fotobiomodulação se mostrou uma modalidade eficaz para o tratamento desta desordem.
{"title":"Fotobiomodulação no tratamento de desordens temporomandibulares","authors":"Fernanda Cristina Nogueira Rodrigues, Júlia Silva Gomes de Araújo, Erika Maria Santos de Araújo, Andrea Dias Neves Lago","doi":"10.11606/ISSN.2357-8041.CLRD.2019.157692","DOIUrl":"https://doi.org/10.11606/ISSN.2357-8041.CLRD.2019.157692","url":null,"abstract":"Objetivo: Relatar um caso clínico utilizando a terapia de fotobiomodulação no tratamento da disfunção temporomandibular proporcionando analgesia e maior abertura bucal. Relato de caso: Paciente, gênero feminino, 24 anos e portadora de disfunção temporomandibular, foi atendida para tratamento no projeto de extensão “Laserterapia em Odontologia” da Universidade Federal do Maranhão. O diagnóstico foi feito através de uma anamnese criteriosa, exame clínico e aplicação de questionário. O aparelho de laser utilizado no tratamento foi o de baixa potência cujo meio ativo é composto por um diodo semicondutor de Arseneto de Gálio e Alumínio (GaAlAs). O protocolo utilizado para o tratamento foi de comprimento de onda 808 nm, 60 s, 6 J de energia por ponto, em um total de dez sessões. Já na segunda sessão, durante a avaliação, a paciente relatou melhora significativa do grau de dor e a medida de abertura bucal variou de 25 mm a 40 mm. Conclusão: A fotobiomodulação se mostrou uma modalidade eficaz para o tratamento desta desordem.","PeriodicalId":10204,"journal":{"name":"Clinical and Laboratorial Research in Dentistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74977412","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-06-25DOI: 10.11606/ISSN.2357-8041.CLRD.2019.154506
A. B. Matos, Bruno Bachiega da Silva, Erika Maria Santos de Araújo, B. F. Silva
Objetivo: Analisar efeitos de diferentes envelhecimentos sobre a rugosidade e a cor de cimentos de ionômero de vidro (CIV) encapsulados. Materiais e métodos: Foram testados dois cimentos de ionômero de vidro [convencional/ Self Cure (SC) e resino modificado/Light Cure (LC)] e envelhecimentos [(baseline, pós-ciclagem térmica e pós- -ciclagem térmica + armazenamento por sete meses)]. 52 discos (n = 13) foram confeccionados. A cor foi avaliada por dois espectrofotômetros (VITA Easyshade e Konica Minolta CE3700A), e a rugosidade pelo perfilômetro óptico (Proscan 2100, Scantron). Resultados: ANOVA dois fatores e teste de Tukey (p < 0,05) foram realizados. Os CIVs apresentaram diferenças estatisticamente significantes entre si (p < 0,05) para rugosidade (SC = 0,202 μm e LC = 0,241), os envelhecimentos alteraram significativamente a rugosidade baseline = 0,278 μm, ciclado = 0,220 μm e ciclado-armazenado = 0,167 μm, e para cor (SC ΔE = 3,89 e o LC ΔE = 4,94). Para ΔL*, Δa*, Δb* houve diferença na interação dos fatores, com maior alteração do ΔL* para o CIV LC após ciclagem + armazenagem. Para Δa* houve diferença estatisticamente significante (p < 0,05) entre os envelhecimentos, sendo maior para o grupo CIV LC após ciclagem. Maior alteração Δb* foi observada para o CIV LC após ciclagem + armazenagem. Conclusões: Os diferentes CIVs possuem rugosidade superficial e cor diferentes. O CIV LC mostrou-se mais rugoso quando comparado ao SC, porém ambos se tornaram menos rugosos com o passar do tempo. O protocolo de envelhecimento que mais interfere na rugosidade e na cor de diferentes tipos de CIV é a ciclagem térmica seguida de armazenamento.
{"title":"Influência do envelhecimento sobre a rugosidade e estabilidade de cor dos cimentos de ionômero de vidro encapsulados","authors":"A. B. Matos, Bruno Bachiega da Silva, Erika Maria Santos de Araújo, B. F. Silva","doi":"10.11606/ISSN.2357-8041.CLRD.2019.154506","DOIUrl":"https://doi.org/10.11606/ISSN.2357-8041.CLRD.2019.154506","url":null,"abstract":"Objetivo: Analisar efeitos de diferentes envelhecimentos sobre a rugosidade e a cor de cimentos de ionômero de vidro (CIV) encapsulados. Materiais e métodos: Foram testados dois cimentos de ionômero de vidro [convencional/ Self Cure (SC) e resino modificado/Light Cure (LC)] e envelhecimentos [(baseline, pós-ciclagem térmica e pós- -ciclagem térmica + armazenamento por sete meses)]. 52 discos (n = 13) foram confeccionados. A cor foi avaliada por dois espectrofotômetros (VITA Easyshade e Konica Minolta CE3700A), e a rugosidade pelo perfilômetro óptico (Proscan 2100, Scantron). Resultados: ANOVA dois fatores e teste de Tukey (p < 0,05) foram realizados. Os CIVs apresentaram diferenças estatisticamente significantes entre si (p < 0,05) para rugosidade (SC = 0,202 μm e LC = 0,241), os envelhecimentos alteraram significativamente a rugosidade baseline = 0,278 μm, ciclado = 0,220 μm e ciclado-armazenado = 0,167 μm, e para cor (SC ΔE = 3,89 e o LC ΔE = 4,94). Para ΔL*, Δa*, Δb* houve diferença na interação dos fatores, com maior alteração do ΔL* para o CIV LC após ciclagem + armazenagem. Para Δa* houve diferença estatisticamente significante (p < 0,05) entre os envelhecimentos, sendo maior para o grupo CIV LC após ciclagem. Maior alteração Δb* foi observada para o CIV LC após ciclagem + armazenagem. Conclusões: Os diferentes CIVs possuem rugosidade superficial e cor diferentes. O CIV LC mostrou-se mais rugoso quando comparado ao SC, porém ambos se tornaram menos rugosos com o passar do tempo. O protocolo de envelhecimento que mais interfere na rugosidade e na cor de diferentes tipos de CIV é a ciclagem térmica seguida de armazenamento.","PeriodicalId":10204,"journal":{"name":"Clinical and Laboratorial Research in Dentistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80944684","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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