Background: Liver fibrosis is scar tissue that forms due to excessive accumulation of ECM proteins due to chronic liver injury, mediated by cytokines involving complex cellular interactions, such as platelet-derived growth factor-BB (PDGF-BB). In Indonesia, no research has been conducted to determine the correlation between PDGF-BB levels and the degree of liver fibrosis based on fibroscan. The aim of this study is to assess the correlation between PGDF-BB levels and the degree of liver fibrosis based on fibroscan in chronic hepatitis B. Methods: A cross-sectional study was conducted on the chronic hepatitis B population at the internal medicine clinic of Dr. Mohammad Hoesin General Hospital Palembang from January to Agustus 2023. The assessment uses fibroscan to determine the degree of liver fibrosis, measured in kilopascals (kPa). PDGF-BB levels measured by ELISA. Results: From 40 subjects, the majority of chronic hepatitis B patients were women (57,5%). The lowest PDGF-BB level was found in chronic hepatitis B patients with fibroscan F0, and the highest PDGF-BB level in fibroscan F4. Fibroscan results are directly proportional to PDGF-BB level. Conclusion: PDGF-BB has a strong positive correlation with the degree of fibroscan in chronic hepatitis B patients in Dr. Mohammad Hoesin General Hospital. The higher the range of PDGF-BB levels, the higher the degree of fibroscan in chronic hepatitis B patients.
背景:肝纤维化是由于慢性肝损伤引起的ECM蛋白过度积累而形成的疤痕组织，由涉及复杂细胞相互作用的细胞因子介导，如血小板衍生生长因子- bb (PDGF-BB)。在印度尼西亚，没有研究基于纤维扫描来确定PDGF-BB水平与肝纤维化程度之间的相关性。本研究的目的是基于纤维扫描评估慢性乙型肝炎患者PGDF-BB水平与肝纤维化程度的相关性。方法:对Palembang Dr. Mohammad Hoesin General Hospital内科门诊的慢性乙型肝炎患者进行横断面研究。评估使用纤维扫描来确定肝纤维化的程度，以千帕斯卡(kPa)为单位。ELISA测定PDGF-BB水平。结果:在40名受试者中，大多数慢性乙型肝炎患者为女性(57,5%)。慢性乙型肝炎患者纤维扫描F0组PDGF-BB水平最低，纤维扫描F4组PDGF-BB水平最高。纤维扫描结果与PDGF-BB水平成正比。结论:PDGF-BB与Dr. Mohammad Hoesin总医院慢性乙型肝炎患者纤维扫描程度呈正相关。PDGF-BB水平越高，慢性乙型肝炎患者纤维扫描程度越高。
{"title":"Correlation of Platelet-Derived Growth Factor-BB Levels as a Biomarker of Liver Fibrosis with the Degree of Fibrosis Based on Fibroscan in Chronic Hepatitis B Patients","authors":"Richard Togi, Lumban Tobing, M. Saleh","doi":"10.37275/bsm.v8i1.913","DOIUrl":"https://doi.org/10.37275/bsm.v8i1.913","url":null,"abstract":"Background: Liver fibrosis is scar tissue that forms due to excessive accumulation of ECM proteins due to chronic liver injury, mediated by cytokines involving complex cellular interactions, such as platelet-derived growth factor-BB (PDGF-BB). In Indonesia, no research has been conducted to determine the correlation between PDGF-BB levels and the degree of liver fibrosis based on fibroscan. The aim of this study is to assess the correlation between PGDF-BB levels and the degree of liver fibrosis based on fibroscan in chronic hepatitis B. \u0000Methods: A cross-sectional study was conducted on the chronic hepatitis B population at the internal medicine clinic of Dr. Mohammad Hoesin General Hospital Palembang from January to Agustus 2023. The assessment uses fibroscan to determine the degree of liver fibrosis, measured in kilopascals (kPa). PDGF-BB levels measured by ELISA. \u0000Results: From 40 subjects, the majority of chronic hepatitis B patients were women (57,5%). The lowest PDGF-BB level was found in chronic hepatitis B patients with fibroscan F0, and the highest PDGF-BB level in fibroscan F4. Fibroscan results are directly proportional to PDGF-BB level. \u0000Conclusion: PDGF-BB has a strong positive correlation with the degree of fibroscan in chronic hepatitis B patients in Dr. Mohammad Hoesin General Hospital. The higher the range of PDGF-BB levels, the higher the degree of fibroscan in chronic hepatitis B patients.","PeriodicalId":102064,"journal":{"name":"Bioscientia Medicina : Journal of Biomedicine and Translational Research","volume":" 29","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138620590","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Background: Neurofibromatosis type 1 (NF-1) is one of the hereditary neurocutaneous disorders. Neurofibromatosis type 1 has broad clinical manifestations that can occur in the skin, bones, eyes, and nervous system. In progressive cases, NF-1 can malign and cause intracerebral tumor lesions. Astrocytoma, especially pilocytic astrocytoma, is one of the tumor classes that patients with NF-1 can develop with far-reaching disease progression. This case report presents a case of NF-1 with intracerebral astrocytoma. Case presentation: A 63-year-old man with whole body lumps since childhood presented with weakness in the right limbs 1 month ago. 3 months earlier, the patient also had a full-body seizure for 1 minute twice. The patient then underwent a magnetic resonance imaging (MRI) examination of the brain and found a mass in the left centrum semiovale, left corona radiata, left insular, left external capsule, and left frontotemporoparietal lobe with a size of 7.2 x 5.3 x 6.2 cm. The patient then underwent a VP shunt followed by tumor resection. The tumor was successfully evacuated, and histopathological examination with hematoxylin & eosin (HE) staining revealed glioblastoma multiformis. The patient also underwent histopathologic examination with Ki-67 immunohistochemistry (IHC) staining and showed pilocytic astrocytoma. Conclusion: Craniotomy of tumor excision is the definitive therapy in neurofibromatosis patients accompanied by intracerebral astrocytoma.
{"title":"A 63-Year-Old Man with Neurofibromatosis Type 1 and Intracerebral Astrocytoma: A Case Report","authors":"Iman Hakim Wicaksana, Geizar Arsika Ramadhana","doi":"10.37275/bsm.v8i1.911","DOIUrl":"https://doi.org/10.37275/bsm.v8i1.911","url":null,"abstract":"Background: Neurofibromatosis type 1 (NF-1) is one of the hereditary neurocutaneous disorders. Neurofibromatosis type 1 has broad clinical manifestations that can occur in the skin, bones, eyes, and nervous system. In progressive cases, NF-1 can malign and cause intracerebral tumor lesions. Astrocytoma, especially pilocytic astrocytoma, is one of the tumor classes that patients with NF-1 can develop with far-reaching disease progression. This case report presents a case of NF-1 with intracerebral astrocytoma. Case presentation: A 63-year-old man with whole body lumps since childhood presented with weakness in the right limbs 1 month ago. 3 months earlier, the patient also had a full-body seizure for 1 minute twice. The patient then underwent a magnetic resonance imaging (MRI) examination of the brain and found a mass in the left centrum semiovale, left corona radiata, left insular, left external capsule, and left frontotemporoparietal lobe with a size of 7.2 x 5.3 x 6.2 cm. The patient then underwent a VP shunt followed by tumor resection. The tumor was successfully evacuated, and histopathological examination with hematoxylin & eosin (HE) staining revealed glioblastoma multiformis. The patient also underwent histopathologic examination with Ki-67 immunohistochemistry (IHC) staining and showed pilocytic astrocytoma. Conclusion: Craniotomy of tumor excision is the definitive therapy in neurofibromatosis patients accompanied by intracerebral astrocytoma.","PeriodicalId":102064,"journal":{"name":"Bioscientia Medicina : Journal of Biomedicine and Translational Research","volume":"46 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-11-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139213330","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Background: Osteogenesis imperfecta (OI), known as brittle bone disease, is a rare inherited and genetic skeletal disorder characterized by a significantly heightened risk of fragility fractures. About 1 in 15 to 20.000 births are affected with OI. these defects may moreover be combined with an airway that's troublesome to oversee, platelet dysfunctions, and other issues. The perioperative administration of anesthesia speaks to a significant challenge. Subsequently, it is critical to be able to anticipate the dangers during the perioperative period and to define and actualize particular high-quality anesthesia administration plans for such patients when they involve injury. Case presentation: We present a case of a female patient 26 years old with known OI type IV who underwent Osteotomy and Open Reduction Internal Fixation with plate and screw (ORIF PS) on her left Tibia and Fibula. In this case, we present the anesthesia technique with general anesthesia using a metal wire-enforced endotracheal tube to prevent partial obstruction due to kyphoscoliosis. Conclusion: It is crucial to assess the severity of the disease, identify risk factors in advance, and optimize the preoperative health status of individuals with OI.
背景：成骨不全症（OI），又称脆骨病，是一种罕见的遗传性骨骼疾病，其特点是脆性骨折的风险显著增加。大约每 15 到 20,000 名新生儿中就有 1 人患有成骨不全症。这些缺陷还可能合并有难以控制的气道、血小板功能障碍和其他问题。围手术期的麻醉管理是一项重大挑战。因此，能够预测围手术期的危险，并在此类患者受伤时为其制定和实施特定的高质量麻醉管理计划至关重要。 病例介绍：我们介绍了一例 26 岁女性患者的病例，她患有已知的 OI IV 型，接受了左胫骨和腓骨的截骨术和带钢板和螺钉的开放还原内固定术（ORIF PS）。在本病例中，我们介绍了使用金属丝加固气管导管进行全身麻醉的麻醉技术，以防止因脊柱侧凸造成部分阻塞。 结论：评估疾病的严重程度、提前识别风险因素并优化 OI 患者的术前健康状况至关重要。
{"title":"Management of Anesthesia in Patient with Osteogenesis Imperfecta Type IV and Fractures on Tibia and Fibula: A Case Report","authors":"Ignatio Armando Kenzi, Otniel Adrians Labobar","doi":"10.37275/bsm.v8i1.908","DOIUrl":"https://doi.org/10.37275/bsm.v8i1.908","url":null,"abstract":"Background: Osteogenesis imperfecta (OI), known as brittle bone disease, is a rare inherited and genetic skeletal disorder characterized by a significantly heightened risk of fragility fractures. About 1 in 15 to 20.000 births are affected with OI. these defects may moreover be combined with an airway that's troublesome to oversee, platelet dysfunctions, and other issues. The perioperative administration of anesthesia speaks to a significant challenge. Subsequently, it is critical to be able to anticipate the dangers during the perioperative period and to define and actualize particular high-quality anesthesia administration plans for such patients when they involve injury. Case presentation: We present a case of a female patient 26 years old with known OI type IV who underwent Osteotomy and Open Reduction Internal Fixation with plate and screw (ORIF PS) on her left Tibia and Fibula. In this case, we present the anesthesia technique with general anesthesia using a metal wire-enforced endotracheal tube to prevent partial obstruction due to kyphoscoliosis. Conclusion: It is crucial to assess the severity of the disease, identify risk factors in advance, and optimize the preoperative health status of individuals with OI.","PeriodicalId":102064,"journal":{"name":"Bioscientia Medicina : Journal of Biomedicine and Translational Research","volume":"46 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-11-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139215817","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Kadek Fajar, Aan Jaya Kusuma, Ida Bagus, Aditya Mayanda, IB Yudhistira, Anantasurya Vidhisvara, Juventius Anggoa
Background: Patients with pelvic floor dysfunction (PFD) who fail conservative treatment will undergo surgery. In recent years, various therapeutic approaches for PFD using stem cells have been investigated in vivo. One component of stem cells is the human umbilical cord mesenchymal stem cell (HUMSC). It is hoped that HUMSC can modify the collagen and elastin content in the extracellular matrix, leading to improvements in pelvic floor strength and PFD. Methods: This research is an experimental study with a randomized posttest-only controlled group design with the aim of determining the effect of giving HUMSC on the expression of Collagen I in a female mouse model of menopause. There are inclusion criteria, namely female Balb/c mice, healthy, never mated, aged 8 - 12 weeks, and body weight 18 - 22 grams. The research will be carried out with statistical analysis using SPSS; a p-value <0.05 is considered significant. Results: The results of the analysis of the distribution of sample means show that there are significant differences between the two groups. The treatment group had a higher mean expression of type I collagen compared to the control group. In addition, variation in the control group was lower compared with the treatment group, which could be interpreted as higher consistency in the response to HUMSC treatment. Conclusion: Human umbilical cord (HUMSC) has a positive impact on type I collagen expression in menopausal model mice.
{"title":"The Effect of Giving Human Umbilical Cord Mesenchymal Stem Cells (HUMSC) on the Expression of Collagen I in Menopause Model Mice","authors":"Kadek Fajar, Aan Jaya Kusuma, Ida Bagus, Aditya Mayanda, IB Yudhistira, Anantasurya Vidhisvara, Juventius Anggoa","doi":"10.37275/bsm.v8i1.905","DOIUrl":"https://doi.org/10.37275/bsm.v8i1.905","url":null,"abstract":"Background: Patients with pelvic floor dysfunction (PFD) who fail conservative treatment will undergo surgery. In recent years, various therapeutic approaches for PFD using stem cells have been investigated in vivo. One component of stem cells is the human umbilical cord mesenchymal stem cell (HUMSC). It is hoped that HUMSC can modify the collagen and elastin content in the extracellular matrix, leading to improvements in pelvic floor strength and PFD. Methods: This research is an experimental study with a randomized posttest-only controlled group design with the aim of determining the effect of giving HUMSC on the expression of Collagen I in a female mouse model of menopause. There are inclusion criteria, namely female Balb/c mice, healthy, never mated, aged 8 - 12 weeks, and body weight 18 - 22 grams. The research will be carried out with statistical analysis using SPSS; a p-value <0.05 is considered significant. Results: The results of the analysis of the distribution of sample means show that there are significant differences between the two groups. The treatment group had a higher mean expression of type I collagen compared to the control group. In addition, variation in the control group was lower compared with the treatment group, which could be interpreted as higher consistency in the response to HUMSC treatment. Conclusion: Human umbilical cord (HUMSC) has a positive impact on type I collagen expression in menopausal model mice.","PeriodicalId":102064,"journal":{"name":"Bioscientia Medicina : Journal of Biomedicine and Translational Research","volume":"78 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-11-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139231623","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Nyoman Ananda, Putri Prashanti, Kanthi Soraca Widiatmika, Putu Siska Suryaningsih, Wayan Bikin Suryawan
Background: Advances in neonatology have led to many preterm infants being saved. Prolonged length of stay (LOS) increases the incidence of neonatal complications and even mortality, placing a significant economic burden on families and strain on healthcare systems. This study aims to determine the factors associated with LOS in preterm infants. Methods: This cross-sectional study involved 60 preterm babies treated at Wangaya Hospital from May 2022 to May 2023. Infants with congenital anomalies, referred from other hospitals and did not receive appropriate treatment were excluded from the study. Data were taken from the electronic medical record after obtaining ethical clearance and then analyzed using descriptive statistics and linear regression in SPSS 25. Statistical significance was considered at 0.05. Results: As many as 46 out of 60 preterm infants were born at moderate to late preterm gestational age. The majority of 81.7% of infants were born with a body weight of 1500-2499 grams. The median number of LOS was 7.5 days (IQR 15). Multivariate analysis of numerical independent variables obtained an equation log [LOS] = 2.902-0.066*gestational age+ 0.022*duration of parenteral nutrition (R2 = 78.1%). Meanwhile, in the categorical independent variables obtained, an equation log [LOS] = 0.398 + 0.223*very preterm + 0.144* RDS + 0.178*NEC + 0.206*prolonged antibiotics administration + 0.278*late enteral feeding + 0.148*abdominal distension + 0.144*vomiting (R2 = 87.4%). Conclusion: Factors influencing LOS of preterm infants are gestational age, duration of parenteral nutrition, the presence of RDS, NEC, vomiting, abdominal distension, late enteral feeding, and prolonged use of antibiotics. It is essential to carry out antenatal care for pregnant women to prevent premature birth.
{"title":"Risk Factors Affecting Length of Stay in Preterm Infants at Wangaya Regional General Hospital, Indonesia","authors":"Nyoman Ananda, Putri Prashanti, Kanthi Soraca Widiatmika, Putu Siska Suryaningsih, Wayan Bikin Suryawan","doi":"10.37275/bsm.v8i1.907","DOIUrl":"https://doi.org/10.37275/bsm.v8i1.907","url":null,"abstract":"Background: Advances in neonatology have led to many preterm infants being saved. Prolonged length of stay (LOS) increases the incidence of neonatal complications and even mortality, placing a significant economic burden on families and strain on healthcare systems. This study aims to determine the factors associated with LOS in preterm infants. Methods: This cross-sectional study involved 60 preterm babies treated at Wangaya Hospital from May 2022 to May 2023. Infants with congenital anomalies, referred from other hospitals and did not receive appropriate treatment were excluded from the study. Data were taken from the electronic medical record after obtaining ethical clearance and then analyzed using descriptive statistics and linear regression in SPSS 25. Statistical significance was considered at 0.05. Results: As many as 46 out of 60 preterm infants were born at moderate to late preterm gestational age. The majority of 81.7% of infants were born with a body weight of 1500-2499 grams. The median number of LOS was 7.5 days (IQR 15). Multivariate analysis of numerical independent variables obtained an equation log [LOS] = 2.902-0.066*gestational age+ 0.022*duration of parenteral nutrition (R2 = 78.1%). Meanwhile, in the categorical independent variables obtained, an equation log [LOS] = 0.398 + 0.223*very preterm + 0.144* RDS + 0.178*NEC + 0.206*prolonged antibiotics administration + 0.278*late enteral feeding + 0.148*abdominal distension + 0.144*vomiting (R2 = 87.4%). Conclusion: Factors influencing LOS of preterm infants are gestational age, duration of parenteral nutrition, the presence of RDS, NEC, vomiting, abdominal distension, late enteral feeding, and prolonged use of antibiotics. It is essential to carry out antenatal care for pregnant women to prevent premature birth.","PeriodicalId":102064,"journal":{"name":"Bioscientia Medicina : Journal of Biomedicine and Translational Research","volume":"15 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-11-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139228577","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Background: Adrenalectomy in patients with coronary arterial disease poses a significant challenge requiring good preoperative evaluation, perioperative hemodynamic control, and a detailed and careful anesthetic strategy. Teratomas are benign tumors that develop from pluripotent cells with two or more germ cell layers. Teratomas can develop in the ovaries and testes. Retroperitoneal teratoma, particularly adrenal teratoma, is rare. Indications for adrenal gland surgery include hormonal secretory tumors and non-hormonal secretory tumors. Case presentation: A 66-year-old man complained of discomfort in the right upper abdomen. From the results of the CT scan, an adrenal teratoma was obtained, and an adrenalectomy operation was planned. The patient underwent adrenalectomy with general anesthesia. Induction was carried out with propofol titrating doses of 100-150 mg until the patient was hypnotized and intubated with Atracurium 30 mg IV. The patient underwent an arterial line and CVC with ultrasound guiding during surgery. Conclusion: Anesthetic techniques should aim to maintain a myocardial oxygen supply that is greater than required to avoid ischemia. It is necessary to control perioperative hypertension, hyperglycemia, hypokalemia, and blood cortisol levels and maintain the balance of myocardial oxygen supply.
{"title":"Combined General Anesthesia with Epidural Analgesia as a Modality for Adrenal Teratoma Resection in Patients with Coronary Arterial Disease","authors":"Wibowo Kartiko, T. Senapathi","doi":"10.37275/bsm.v8i1.906","DOIUrl":"https://doi.org/10.37275/bsm.v8i1.906","url":null,"abstract":"Background: Adrenalectomy in patients with coronary arterial disease poses a significant challenge requiring good preoperative evaluation, perioperative hemodynamic control, and a detailed and careful anesthetic strategy. Teratomas are benign tumors that develop from pluripotent cells with two or more germ cell layers. Teratomas can develop in the ovaries and testes. Retroperitoneal teratoma, particularly adrenal teratoma, is rare. Indications for adrenal gland surgery include hormonal secretory tumors and non-hormonal secretory tumors. Case presentation: A 66-year-old man complained of discomfort in the right upper abdomen. From the results of the CT scan, an adrenal teratoma was obtained, and an adrenalectomy operation was planned. The patient underwent adrenalectomy with general anesthesia. Induction was carried out with propofol titrating doses of 100-150 mg until the patient was hypnotized and intubated with Atracurium 30 mg IV. The patient underwent an arterial line and CVC with ultrasound guiding during surgery. Conclusion: Anesthetic techniques should aim to maintain a myocardial oxygen supply that is greater than required to avoid ischemia. It is necessary to control perioperative hypertension, hyperglycemia, hypokalemia, and blood cortisol levels and maintain the balance of myocardial oxygen supply.","PeriodicalId":102064,"journal":{"name":"Bioscientia Medicina : Journal of Biomedicine and Translational Research","volume":"25 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-11-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139230588","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Background: Centella asiatica (CA) or pegagan or horse foot leaves is one of the traditional plants widely used to treat various diseases. This study aims to evaluated the content of total phenolics and total flavonoids, as well as the antioxidant activity of Centella asiatica extracts from different methods and solvents. Methods: The sample was divided into three groups, (70% ethanol maceration, methanol maceration and water infundation). Total phenolic compounds in the extract were determined by the Follin-Ciocalteu method, total flavonoid content was determined by the aluminum chloride colorimetric assay method, and antioxidant activity was assessed by the immersion DPPH free radicals method. Determination data of IC50 was assessed by regression test and differences between the three extract groups were tested by ANOVA or Kruskal Wallis test. Results: The highest total phenolics and total flavonoid were obtained in Centella asiatica extract with water solvent, 251.88 ± 0.96 mg/g GAE and 7.26 ± 0.03 mg/g QE, followed by 70% ethanol and methanol. In addition, the best antioxidant activity was also shown in the extract with water, namely 24.09 ± 0.01. The difference test results between all groups showed significant results (p<0.05) in all parameters. Conclusion: The best content of total phenolic, total flavonoid, and antioxidant activity was obtained by infundation with water solvent. In the maceration method, the best content of the three parameters measured was obtained in the group with ethanol solvent.
{"title":"Effect of Extraction Method and Solvent Type on Total Phenolics Content, Total Flavonoid and Antioxidant Activity of Pegagan Extract (Centella asiatica (Linn.) Urban)","authors":"Ahyar Riza, Syafruddin Ilyas, Endang Sjamsudin, Delyuzar Delyuzar","doi":"10.37275/bsm.v8i1.902","DOIUrl":"https://doi.org/10.37275/bsm.v8i1.902","url":null,"abstract":"Background: Centella asiatica (CA) or pegagan or horse foot leaves is one of the traditional plants widely used to treat various diseases. This study aims to evaluated the content of total phenolics and total flavonoids, as well as the antioxidant activity of Centella asiatica extracts from different methods and solvents. Methods: The sample was divided into three groups, (70% ethanol maceration, methanol maceration and water infundation). Total phenolic compounds in the extract were determined by the Follin-Ciocalteu method, total flavonoid content was determined by the aluminum chloride colorimetric assay method, and antioxidant activity was assessed by the immersion DPPH free radicals method. Determination data of IC50 was assessed by regression test and differences between the three extract groups were tested by ANOVA or Kruskal Wallis test. Results: The highest total phenolics and total flavonoid were obtained in Centella asiatica extract with water solvent, 251.88 ± 0.96 mg/g GAE and 7.26 ± 0.03 mg/g QE, followed by 70% ethanol and methanol. In addition, the best antioxidant activity was also shown in the extract with water, namely 24.09 ± 0.01. The difference test results between all groups showed significant results (p<0.05) in all parameters. Conclusion: The best content of total phenolic, total flavonoid, and antioxidant activity was obtained by infundation with water solvent. In the maceration method, the best content of the three parameters measured was obtained in the group with ethanol solvent.","PeriodicalId":102064,"journal":{"name":"Bioscientia Medicina : Journal of Biomedicine and Translational Research","volume":"223 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-11-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139241255","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Jersivindo Ranazeri, Rose Dinda Martini, Roza Mulyana, Fandi Triansyah
Osteosarcopenia is a syndrome defined as a combination of low bone density and muscle mass as well as decreased strength and/or functional capacity. Osteoporosis and sarcopenia often coexist in the elderly, leading to a significantly worse prognosis. The epidemiology of osteosarcopenia is quite limited because the term is still new. Osteosarcopenia is more common in women than men and in malnutrition. Age-related immunological changes such as hormonal imbalance, chronic inflammation, increased oxidative stress, imbalance in protein metabolism, increased fat deposition, decreased physical activity, and poor nutritional status contribute to sarcopenia. Decreased bone density in osteoporosis can occur due to an imbalance between osteoblasts and osteoclasts. The diagnosis of osteosarcopenia is made based on the presence of osteoporosis and sarcopenia. Instrument strength, assistance with walking, rising from a chair, climbing stairs, and falls (SARC–F) are recommended for assessing sarcopenia and the fracture risk assessment tool (FRAX) for the risk of osteoporotic fracture. Management of osteosarcopenia is carried out holistically, including management of osteoporosis and sarcopenia both non-pharmacologically and pharmacologically.
{"title":"Diagnosis and Management of Osteosarcopenia","authors":"Jersivindo Ranazeri, Rose Dinda Martini, Roza Mulyana, Fandi Triansyah","doi":"10.37275/bsm.v8i1.904","DOIUrl":"https://doi.org/10.37275/bsm.v8i1.904","url":null,"abstract":"Osteosarcopenia is a syndrome defined as a combination of low bone density and muscle mass as well as decreased strength and/or functional capacity. Osteoporosis and sarcopenia often coexist in the elderly, leading to a significantly worse prognosis. The epidemiology of osteosarcopenia is quite limited because the term is still new. Osteosarcopenia is more common in women than men and in malnutrition. Age-related immunological changes such as hormonal imbalance, chronic inflammation, increased oxidative stress, imbalance in protein metabolism, increased fat deposition, decreased physical activity, and poor nutritional status contribute to sarcopenia. Decreased bone density in osteoporosis can occur due to an imbalance between osteoblasts and osteoclasts. The diagnosis of osteosarcopenia is made based on the presence of osteoporosis and sarcopenia. Instrument strength, assistance with walking, rising from a chair, climbing stairs, and falls (SARC–F) are recommended for assessing sarcopenia and the fracture risk assessment tool (FRAX) for the risk of osteoporotic fracture. Management of osteosarcopenia is carried out holistically, including management of osteoporosis and sarcopenia both non-pharmacologically and pharmacologically.","PeriodicalId":102064,"journal":{"name":"Bioscientia Medicina : Journal of Biomedicine and Translational Research","volume":"37 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-11-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139244703","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Background: Anesthesia management in morbidly obese patients undergoing any neurosurgical procedure is particularly challenging. This case report aims to further discuss the importances and concerns regarding the anesthesia management of decompression craniectomy in morbidly obese patients. Case presentation: A 46-years-old male patient was diagnosed with intracerebral hemorrhage on regio left external capsule due to hypertensive emergency suspected from Middle Cerebral Artery (MCA) aneurysma rupture. Decompression craniectomy with clot evacuation was planned. This patient has morbid obesity. Prediction of difficult intubation and mask ventilation can be assessed using LEMONS, MOANS, Mallampati score and STOP-Bang Questionnaire. The dosage of anesthetic agents used in these patients had been adjusted according to current recommendations. Intraoperatively, patient’s hemodynamic was successfully maintained stable. Postoperatively, patient was closely monitored in the intensive care unit. Conclusion: It is imperative to acquire adequate knowledge on choosing the effective anesthetic management during the preoperative, intraoperative, and postoperative periods to achieve good outcome in morbidly obese patient undergoing neurosurgical procedures.
{"title":"Anesthesia Management in Morbidly Obese Patient Undergoing Neurosurgical Procedure: A Case Report","authors":"Charles, Cynthia Dewi Sinardja","doi":"10.37275/bsm.v7i12.903","DOIUrl":"https://doi.org/10.37275/bsm.v7i12.903","url":null,"abstract":"Background: Anesthesia management in morbidly obese patients undergoing any neurosurgical procedure is particularly challenging. This case report aims to further discuss the importances and concerns regarding the anesthesia management of decompression craniectomy in morbidly obese patients. Case presentation: A 46-years-old male patient was diagnosed with intracerebral hemorrhage on regio left external capsule due to hypertensive emergency suspected from Middle Cerebral Artery (MCA) aneurysma rupture. Decompression craniectomy with clot evacuation was planned. This patient has morbid obesity. Prediction of difficult intubation and mask ventilation can be assessed using LEMONS, MOANS, Mallampati score and STOP-Bang Questionnaire. The dosage of anesthetic agents used in these patients had been adjusted according to current recommendations. Intraoperatively, patient’s hemodynamic was successfully maintained stable. Postoperatively, patient was closely monitored in the intensive care unit. Conclusion: It is imperative to acquire adequate knowledge on choosing the effective anesthetic management during the preoperative, intraoperative, and postoperative periods to achieve good outcome in morbidly obese patient undergoing neurosurgical procedures.","PeriodicalId":102064,"journal":{"name":"Bioscientia Medicina : Journal of Biomedicine and Translational Research","volume":"37 4","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-11-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139246916","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Alamanda Murasmita, Meirina Mulia, Maria Galuh, Dita Eka, Ervina Rosmarwati
Background: Indonesia has reported more than 4 million confirmed COVID-19 cases as of November 2021. This has led to an increase in the use of personal protective equipment (PPE), for example, surgical masks in hospitals, especially referral hospitals for COVID-19 patients. The Potential Hydrogen (pH) or level of acidity plays an important role in the body's physiological functions and regulates the formation of an epidermal barrier (stratum corneum). Thus, the use of surgical masks is able to influence the pH of human skin. This study aimed to determine the difference in facial skin acidity (pH) levels of nurses at Sebelas Maret University Hospital before and after surgical mask use. Methods: The study utilized a cross-sectional observational method and was conducted at the Sebelas Maret University Hospital, Surakarta. A total of 55 subjects were involved in this study, and each subject’s facial skin Potential Hydrogen (pH) was measured before and after surgical mask use. Subsequently, all data were analyzed with a T-test using SPSS 21.00, and a p-value <0.05 indicated statistical significance. Results: The results showed facial skin pH increased after surgical mask use for nurses at the Sebelas Maret University Hospital (p = 0.000). Conclusion: An increase in the physiological value of skin pH was observed in the nurses of Sebelas Maret University Hospital after surgical mask use.
背景：截至 2021 年 11 月，印度尼西亚报告的 COVID-19 确诊病例已超过 400 万例。这导致个人防护设备（PPE）的使用增加，例如在医院，特别是COVID-19患者的转诊医院使用外科口罩。潜在氢（pH 值）或酸度在人体生理功能中发挥着重要作用，并调节表皮屏障（角质层）的形成。因此，使用外科口罩会影响人体皮肤的 pH 值。本研究旨在确定塞贝拉斯马雷特大学医院护士在使用手术面罩前后面部皮肤酸度（pH 值）的差异。 研究方法研究采用横断面观察法，在苏腊卡尔塔的 Sebelas Maret 大学医院进行。共有 55 名受试者参与了这项研究，并在使用手术面罩前后测量了每位受试者面部皮肤的氢电位（pH 值）。随后，使用 SPSS 21.00 对所有数据进行了 T 检验分析，P 值小于 0.05 表示具有统计学意义。 结果显示结果显示，Sebelas Maret 大学医院的护士在使用手术面罩后，面部皮肤 pH 值升高（p = 0.000）。 结论：Sebelas Maret 大学医院的护士在使用外科口罩后，皮肤 pH 值的生理值有所增加。
{"title":"Differences in Facial Skin Acidity Levels of Nurses in Sebelas Maret University Hospital, Before and After Surgical Mask Use During the COVID-19 Pandemic","authors":"Alamanda Murasmita, Meirina Mulia, Maria Galuh, Dita Eka, Ervina Rosmarwati","doi":"10.37275/bsm.v7i12.897","DOIUrl":"https://doi.org/10.37275/bsm.v7i12.897","url":null,"abstract":"Background: Indonesia has reported more than 4 million confirmed COVID-19 cases as of November 2021. This has led to an increase in the use of personal protective equipment (PPE), for example, surgical masks in hospitals, especially referral hospitals for COVID-19 patients. The Potential Hydrogen (pH) or level of acidity plays an important role in the body's physiological functions and regulates the formation of an epidermal barrier (stratum corneum). Thus, the use of surgical masks is able to influence the pH of human skin. This study aimed to determine the difference in facial skin acidity (pH) levels of nurses at Sebelas Maret University Hospital before and after surgical mask use. Methods: The study utilized a cross-sectional observational method and was conducted at the Sebelas Maret University Hospital, Surakarta. A total of 55 subjects were involved in this study, and each subject’s facial skin Potential Hydrogen (pH) was measured before and after surgical mask use. Subsequently, all data were analyzed with a T-test using SPSS 21.00, and a p-value <0.05 indicated statistical significance. Results: The results showed facial skin pH increased after surgical mask use for nurses at the Sebelas Maret University Hospital (p = 0.000). Conclusion: An increase in the physiological value of skin pH was observed in the nurses of Sebelas Maret University Hospital after surgical mask use.","PeriodicalId":102064,"journal":{"name":"Bioscientia Medicina : Journal of Biomedicine and Translational Research","volume":"06 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-11-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139267770","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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