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Essential role of interferon-regulatory factor 4 in regulating diabetogenic CD4+ T and innate immune cells in autoimmune diabetes in NOD mice. 干扰素调节因子 4 在 NOD 小鼠自身免疫性糖尿病中调节致糖尿病 CD4+ T 细胞和先天性免疫细胞的重要作用。
IF 3.4 3区 医学 Q3 IMMUNOLOGY Pub Date : 2024-10-21 DOI: 10.1093/cei/uxae093
Tetsuro Niri, Shin-Ichi Inoue, Satoru Akazawa, Shinpei Nishikido, Masaki Miwa, Masakazu Kobayashi, Katsuyuki Yui, Minoru Okita, Atsushi Kawakami, Norio Abiru

Haploinsufficiency of the transcription factor interferon-regulatory factor 4 (IRF4) prevents the onset of spontaneous diabetes in NOD mice. However, the immunological mechanisms of the IRF4-mediated disease regulation remain unclear. This study aims to investigate the role of IRF4 in the pathogenesis of autoimmune diabetes by conducting adoptive transfer experiments using donor IRF4 gene-deficient CD4+ T cells from BDC2.5-transgenic (Tg) NOD mice and recipient Rag1-knockout NOD mice, respectively. Through this approach, we analyzed both clinical and immunological phenotypes of the recipient mice. Additionally, IRF4-deficient BDC2.5 CD4+ T cells were stimulated to assess their immunological and metabolic phenotypes in vitro. The findings revealed that diabetes was completely prevented in the recipients with Irf4-/- T cells and was approximately 50% lower in those with Irf4+/- T cells than in wild type (WT) controls, whereas Irf4-/- recipients with WT T cells only showed a delayed onset of diabetes. Islet-infiltrating T cells isolated from recipients with Irf4+/- T cells exhibited significantly lower proliferation and IFN-γ/IL-17 double-positive cell fraction rates compared with those in WT controls. Irf4-/- BDC2.5 CD4+ T cells stimulated in vitro showed a reduced number of cell divisions, decreased antigen-specific T-cell markers, and impairment of glycolytic capacity compared with those observed in WT controls. We concluded that IRF4 predominantly regulates the diabetogenic potential in a dose-dependent manner by mediating the proliferation and differentiation of islet-infiltrating T cells while playing an adjunctive role in the innate immune responses toward diabetes progression in NOD mice.

转录因子干扰素调节因子 4(IRF4)的单倍体缺陷可预防 NOD 小鼠自发性糖尿病的发生。然而,IRF4介导疾病调控的免疫学机制仍不清楚。本研究旨在通过分别使用来自BDC2.5转基因(Tg)NOD小鼠和受体Rag1基因敲除NOD小鼠的供体IRF4基因缺陷CD4+ T细胞进行收养转移实验,研究IRF4在自身免疫性糖尿病发病机制中的作用。通过这种方法,我们分析了受体小鼠的临床和免疫表型。此外,我们还刺激了IRF4缺陷的BDC2.5 CD4+ T细胞,以评估它们在体外的免疫和代谢表型。研究结果表明,Irf4-/- T细胞受体完全避免了糖尿病的发生,与野生型(WT)对照组相比,Irf4+/- T细胞受体的糖尿病发病率降低了约50%,而Irf4-/- WT T细胞受体的糖尿病发病率仅有所延迟。与WT对照组相比,从Irf4+/- T细胞受体中分离出的内浸润T细胞的增殖率和IFN-γ/IL-17双阳性细胞率明显较低。与 WT 对照组相比,体外刺激的 Irf4-/- BDC2.5 CD4+ T 细胞表现出细胞分裂次数减少、抗原特异性 T 细胞标记物减少以及糖酵解能力受损。我们的结论是,IRF4主要通过介导胰岛浸润T细胞的增殖和分化,以剂量依赖性方式调节致糖尿病潜能,同时在NOD小鼠糖尿病进展的先天性免疫反应中发挥辅助作用。
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引用次数: 0
Unexplained fever with consumptive syndrome in the elderly: two cases of VEXAS syndrome with inflammasome dysregulation. 老年人不明原因发热伴消耗性综合征:两例炎症小体失调的 VEXAS 综合征。
IF 3.4 3区 医学 Q3 IMMUNOLOGY Pub Date : 2024-10-16 DOI: 10.1093/cei/uxae069
Leonardo Oliveira Mendonça, Vinicius N C Leal, Mariela E G V Roa, Samar Freschi Barros, Jorge Kalil, Alessandra Pontillo

The aim of this study is to investigate the inflammasome dysregulation in peripheral blood leukocytes of VEXAS patients. The constitutive and in vitro triggered activation of inflammasome in PBMC and neutrophils was analyzed in two Brazilian patients with typical UBA1 mutations, and compared with healthy donors. Our findings highlight the constitutive activation of caspase-1 in VEXAS leukocytes, accompanied by increased plasma levels of IL-18. Furthermore, upon stimulation of isolated peripheral blood mononuclear cells (PBMC) and neutrophils, we observed not only the exhaustion of NLRP3 and NLRP1/CARD8 pathways in VEXAS PBMC but also a significant increase in NLRP3-mediated NETs release in VEXAS neutrophils. These findings support previous studies on the contribution of the inflammasome to VEXAS pathogenesis, identifying at least two profoundly affected pathways (NLRP3 and NLRP1/CARD8) in VEXAS peripheral blood.

本研究的目的是调查 VEXAS 患者外周血白细胞中的炎性体失调。研究人员分析了两名典型UBA1突变的巴西患者的外周血白细胞和中性粒细胞中炎症小体的构成性激活和体外触发激活,并与健康供体进行了比较。我们的研究结果表明,VEXAS白细胞中的caspase-1被持续激活,同时血浆中的IL-18水平升高。此外,在对分离的外周血单核细胞(PBMC)和中性粒细胞进行刺激时,我们不仅观察到 VEXAS PBMC 中 NLRP3 和 NLRP1/CARD8 通路的耗竭,还观察到 VEXAS 中性粒细胞中 NLRP3 介导的 NETs 释放显著增加。这些发现支持了之前关于炎性体对VEXAS发病机制的贡献的研究,确定了VEXAS外周血中至少有两种途径(NLRP3和NLRP1/CARD8)受到严重影响。
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引用次数: 0
Identification of angiogenesis-related genes and molecular subtypes for psoriasis based on random forest algorithm. 基于随机森林算法的银屑病血管生成相关基因和分子亚型鉴定
IF 3.4 3区 医学 Q3 IMMUNOLOGY Pub Date : 2024-10-16 DOI: 10.1093/cei/uxae052
Meng-Jie Zhang, Ting-Ting Xue, Xiao-Ya Fei, Ying Zhang, Ying Luo, Yi Ru, Jing-Si Jiang, Jian-Kun Song, Le Kuai, Yue Luo, Rui-Ping Wang, Bin Li

Psoriasis is a chronic immune-mediated recurrent skin disease causing systemic damage. Increased angiogenesis has been reported to participate in the progression of psoriasis. However, angiogenesis-related genes (ARGs) in psoriasis have not been systematically elucidated. Therefore, we aim to identify potential biomarkers and subtypes using two algorithmsr. Transcriptome sequencing data of patients with psoriasis were obtained, in which differentially expressed genes were assessed by principal component analysis. A diagnostic model was developed using random forest algorithm and validated by receiver operating characteristic (ROC) curves. Subsequently, we performed consensus clustering to calculate angiogenesis-associated molecular subtypes of psoriasis. Additionally, a correlation analysis was conducted between ARGs and immune cell infiltration. Finally, validation of potential ARG genes was performed by quantitative real-time PCR (qRT-PCR). We identified 29 differentially expressed ARGs, including 13 increased and 16 decreased. Ten ARGs, CXCL8, ANG, EGF, HTATIP2, ANGPTL4, TNFSF12, RHOB, PML, FOXO4, and EMCN were subsequently sifted by the diagnostic model based on a random forest algorithm. Analysis of the ROC curve (area under the curve [AUC] = 1.0) indicated high diagnostic performance in internal validation. The correlation analysis suggested that CXCL8 has a high positive correlation with neutrophil (R =0.8, P < 0.0001) and interleukins pathway (R = 0.79, P < 0.0001). Furthermore, two ARG-mediated subtypes were obtained, indicating potential heterogeneity. Finally, the qRT-PCR demonstrated that the mRNA expression levels of CXCL8 and ANGPTL4 were elevated in psoriasis patients, with a reduced expression of EMCN observed. The current paper indicated potential ARG-related biomarkers of psoriasis, including CXCL8, ANGPTL4, and EMCN, with two molecular subtypes.

银屑病是一种由免疫介导的慢性复发性皮肤病,会造成全身性损害。据报道,血管生成增加参与了银屑病的进展。然而,银屑病中的血管生成相关基因(ARGs)尚未得到系统阐明。因此,我们旨在利用两种算法确定潜在的生物标志物和亚型。我们获得了银屑病患者的转录组测序数据,并通过主成分分析(PCA)对其中的差异表达基因进行了评估。使用随机森林算法(ntree=400)建立了诊断模型,并通过 ROC 曲线进行了验证。随后,我们进行了共识聚类,计算出与血管生成相关的银屑病分子亚型。此外,我们还进行了 ARG 与免疫细胞浸润之间的相关性分析。最后,通过 qRT-PCR 对潜在的 ARG 基因进行了验证。我们确定了 29 个差异表达的 ARGs,包括 13 个增加的 ARGs 和 16 个减少的 ARGs。随后,基于随机森林算法的诊断模型筛选出了 10 个 ARGs,即 CXCL8、ANG、EGF、HTATIP2、ANGPTL4、TNFSF12、RHOB、PML、FOXO4 和 EMCN。ROC 曲线分析(曲线下面积 [AUC] = 1.0)表明,内部验证的诊断性能很高。相关性分析表明,CXCL8 与中性粒细胞呈高度正相关(R =0.8,P<0.05)。
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引用次数: 0
An update on risk factors for relapse in antineutrophil cytoplasmic antibody-associated vasculitis. 抗中性粒细胞胞浆抗体(ANCA)相关性血管炎复发风险因素的最新进展。
IF 3.4 3区 医学 Q3 IMMUNOLOGY Pub Date : 2024-10-16 DOI: 10.1093/cei/uxae068
Han Zhou, Wei Liang, Hongtu Hu, Zikang Liu, Fan Chu, Guohua Ding

Ongoing therapeutic advances in antineutrophil cytoplasmic antibody-associated vasculitis (AAV) have significantly reduced the risk of death in AAV, but 30%-50% of patients still relapse. Relapse is a major problem in these diseases, leading to increased morbidity and mortality. It is, therefore, necessary to find predictors of relapse at the end of the remission induction and maintenance phases in order to personalize treatment.

抗中性粒细胞胞浆抗体相关性血管炎(AAV)的治疗不断取得进展,大大降低了AAV患者的死亡风险,但仍有30%-50%的患者会复发。复发是这些疾病的一个主要问题,会导致发病率和死亡率上升。因此,有必要找到缓解诱导和维持阶段结束时的复发预测因素,以便进行个性化治疗。
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引用次数: 0
Circulating neutrophil extracellular trap-forming neutrophils in rheumatoid arthritis exacerbation are majority dual endothelin-1/signal peptide receptor+ subtype. 类风湿性关节炎加重期的循环中性粒细胞胞外捕获器(NET)形成的中性粒细胞大多是内皮素-1/信号肽受体(DEspR)+双重亚型。
IF 3.4 3区 医学 Q3 IMMUNOLOGY Pub Date : 2024-10-16 DOI: 10.1093/cei/uxae072
Andrew L Cross, Helen L Wright, Jacqueline Choi, Steven W Edwards, Nelson Ruiz-Opazo, Victoria L M Herrera

Neutrophil extracellular traps (NETs) are associated with rheumatoid arthritis pathogenesis and severity. Since homeostatic NET-forming neutrophils [NET+Ns] have beneficial roles in defense against pathogens, their distinction from pro-injury [NET+N] subtypes is important, especially if they are to be therapeutically targeted. Having identified circulating, pro-injury DEspR+CD11b+[NET+Ns] in patients with neutrophilic secondary tissue injury, we determined whether DEspR+[NET+Ns] are present in rheumatoid arthritis (RA) flares. Whole blood samples of patients with RA flares on maintenance therapy (n = 6) were analyzed by flow cytometry (FCM) and immunofluorescence cytology followed by semi-automated quantitative confocal microscopy (qIFC). We assessed clinical parameters, levels of neutrophils and [NET+Ns], and plasma S100A8/A9. qIFC detected circulating DEspR+CD11b+neutrophils and [NET+Ns] in RA-flare patients but not healthy controls. DEspR+[NET+Ns] were positive for citrullinated histone H3 (citH3+), extruded DNA, decondensed but recognizable polymorphic nuclei, and [NET+N] doublet interactions in mostly non-ruptured NET-forming neutrophils. Circulating DNA+/DEspR+/CD11b+/citH3+microvesicles (netMVs) were observed. FCM detected increased %DEspR+CD11b+neutrophils and DEspR+ cell-cell doublets whose levels trended with DAS28 scores, as did plasma S100A8/A9 levels. This study identifies circulating DEspR+/CD11b+neutrophils and [NET+Ns] in RA-flare patients on maintenance therapy. Detection of circulating DEspR+citH3+[NET+Ns] and netMVs indicate a systemic neutrophilic source of citH3-antigen concordant with multi-joint RA pathogenesis. Increased S100A8/A9 alarmin levels are associated with cell injury and released upon NET-formation. As a ligand for TLR4, S100A8/A9 forms a positive feedback loop for TLR4-induced DEspR+neutrophils. These data identify DEspR+neutrophils and [NET+Ns] in RA pathogenesis as a potential biomarker and/or therapeutic target.

中性粒细胞胞外捕获物(NET)与类风湿性关节炎的发病机制和严重程度有关。由于平衡型NET形成的中性粒细胞[NET+Ns]在抵御病原体方面发挥有益作用,因此将它们与促损伤型[NET+N]亚型区分开来非常重要,尤其是如果要以它们为治疗目标的话。在中性粒细胞继发性组织损伤患者中发现了循环中的促损伤 DEspR+CD11b+ [NET+Ns]后,我们确定了 RA 病变中是否存在 DEspR+ [NET+Ns]。我们通过流式细胞术(FCM)和免疫荧光细胞学方法对接受维持治疗的 RA 发炎患者(6 人)的全血样本进行了分析,然后使用半自动定量共聚焦显微镜(qIFC)进行观察。我们评估了临床参数、中性粒细胞和[NET+Ns]水平以及血浆 S100A8/A9。qIFC 在 RA 发疹患者中检测到了循环中的 DEspR+CD11b+ 中性粒细胞和[NET+Ns],而健康对照组没有检测到。DEspR+[NET+Ns]对瓜氨酸组蛋白H3(citH3+)、挤出的DNA、解聚但可识别的多形性核以及[NET+N]双交互作用呈阳性,其中大部分是非破裂的NET形成的中性粒细胞。观察到循环 DNA+/DEspR+/CD11b+/citH3+ 微囊泡(netMVs)。FCM检测到%DEspR+CD11b+中性粒细胞和DEspR+细胞-细胞双倍体增加,其水平与DAS28评分呈趋势,血浆S100A8/A9水平也是如此。这项研究确定了接受维持治疗的 RA 发炎患者中的循环 DEspR+/CD11b+ 中性粒细胞和 [NET+Ns] 。循环中 DEspR+citH3+ [NET+Ns] 和 netMVs 的检测表明,citH3 抗原的系统性中性粒细胞来源与多关节 RA 发病机制一致。S100A8/A9 alarmin水平的增加与细胞损伤有关,并在NET形成时释放。作为 TLR4 的配体,S100A8/A9 对 TLR4 诱导的 DEspR+ 中性粒细胞形成正反馈回路。这些数据确定了 DEspR+ 中性粒细胞和[NET+Ns]在 RA 发病机制中是一种潜在的生物标记物和/或治疗靶点。
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引用次数: 0
Implementing distinct spatial proteogenomic technologies: opportunities, challenges, and key considerations. 实施独特的空间蛋白质基因组技术;机遇、挑战和主要考虑因素。
IF 3.4 3区 医学 Q3 IMMUNOLOGY Pub Date : 2024-10-16 DOI: 10.1093/cei/uxae077
Bram Verstappe, Charlotte L Scott

Our ability to understand the cellular complexity of tissues has been revolutionized in recent years with significant advances in proteogenomic technologies including those enabling spatial analyses. This has led to numerous consortium efforts, such as the human cell atlas initiative which aims to profile all cells in the human body in healthy and diseased contexts. The availability of such information will subsequently lead to the identification of novel biomarkers of disease and of course therapeutic avenues. However, before such an atlas of any given healthy or diseased tissue can be generated, several factors should be considered including which specific techniques are optimal for the biological question at hand. In this review, we aim to highlight some of the considerations we believe to be important in the experimental design and analysis process, with the goal of helping to navigate the rapidly changing landscape of technologies available.

近年来,随着蛋白质基因组学技术(包括空间分析技术)的显著进步,我们了解组织细胞复杂性的能力发生了革命性的变化。人类细胞图谱(HCA)计划旨在为人体健康和患病情况下的所有细胞绘制图谱。有了这些信息,就能确定疾病的新型生物标志物,当然还能找到治疗途径。然而,在生成任何特定健康或疾病组织的图谱之前,应考虑几个因素,包括哪些特定技术是解决当前生物学问题的最佳方法。在这篇综述中,我们将重点介绍我们认为在实验设计和分析过程中非常重要的一些注意事项,目的是帮助大家驾驭瞬息万变的可用技术。
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引用次数: 0
Inborn Errors of Immunity-related Immunological Mechanisms and Pharmacological Therapy Alternatives in Periodontitis. 牙周炎中与先天性免疫错误相关的免疫机制和药物疗法替代方案。
IF 3.4 3区 医学 Q3 IMMUNOLOGY Pub Date : 2024-10-16 DOI: 10.1093/cei/uxae089
Nadira Nabiyeva Çevik, Ezel Berker, Ilhan Tezcan, Deniz Cagdas

Periodontitis is a frequent local inflammatory disease. The microbiota and repeated exposure to bacterial endotoxins triggers excessive inflammation through oral mucosal immunity, sometimes leading to a destructive effect on the supportive mucosal tissues around the teeth. Elimination of the pathogens and increasing the tolerance of the cellular immune response is crucial in addition to standard dental therapies like mechanical debridement. Based on our experience on immune-mediated diseases, especially primary immunodeficiency diseases (PIDs), we wrote this review to discuss the treatment alternatives for severe periodontal disease. Risk factors are malnutrition, vitamin deficiencies, smoking, systemic inherited and acquired immune-mediated diseases, infections, endocrinological diseases, and pharmacological agents may accompany periodontitis. The diagnosis and treatment of dietary deficiencies, as well as the addition of nutritional supplements, may aid in epithelial regeneration and immune system function. Recently, modifications to the therapeutic option for severe periodontitis have been made depending on the fact that the immune response against bacteria may modify the severity of periodontal inflammation. The anti-inflammatory therapies support or inhibit the host's immune response. The clinical approach to severe periodontitis should extend beyond classical therapies. There is a need for a diverse therapeutic strategy that supports the epithelial barrier, which is the crucial component of innate immunity against microbiota. Leukocytes are the main cellular component in periodontal inflammation. Anti-inflammatory therapeutic options directed at leukocytes, such as IL-17 and IL-23-targeted therapies, could be the candidates for the treatment of severe periodontitis. Therapy against other inflammatory cytokines, IL-1, IL-6, IL12, IL23, TNF-alpha, PGE2, and cytokine receptors, could also be used in periodontal inflammation control.

牙周炎是一种常见的局部炎症性疾病。微生物群和反复接触细菌内毒素会通过口腔黏膜免疫引发过度炎症,有时会对牙齿周围的支持性黏膜组织造成破坏性影响。除了机械清创等标准牙科疗法外,消除病原体和提高细胞免疫反应的耐受性也至关重要。基于我们在免疫介导疾病,尤其是原发性免疫缺陷病(PID)方面的经验,我们撰写了这篇综述,讨论严重牙周病的替代治疗方法。危险因素包括营养不良、维生素缺乏、吸烟、系统性遗传和获得性免疫介导疾病、感染、内分泌疾病,以及可能伴随牙周炎的药物。诊断和治疗饮食不足以及添加营养补充剂可能有助于上皮再生和免疫系统功能。最近,根据针对细菌的免疫反应可能改变牙周炎症的严重程度这一事实,对严重牙周炎的治疗方案进行了修改。消炎疗法支持或抑制宿主的免疫反应。治疗严重牙周炎的临床方法应超越传统疗法。需要一种支持上皮屏障的多样化治疗策略,上皮屏障是对抗微生物群的先天性免疫的重要组成部分。白细胞是牙周炎症的主要细胞成分。针对白细胞的抗炎治疗方案,如 IL-17 和 IL-23 靶向疗法,可能成为治疗严重牙周炎的候选方案。针对其他炎症细胞因子(IL-1、IL-6、IL12、IL23、TNF-α、PGE2 和细胞因子受体)的疗法也可用于牙周炎症的控制。
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引用次数: 0
Protecting children and adults with primary antibody deficiencies against common and emergent pathogens and non-infectious complications. 保护初级抗体缺乏症儿童和成人免受常见病原体、突发病原体和非感染性并发症的侵害。
IF 3.4 3区 医学 Q3 IMMUNOLOGY Pub Date : 2024-10-16 DOI: 10.1093/cei/uxae059
Olaf Neth, Nizar Mahlaoui, Charlotte Cunningham-Rundles

Prevention and treatment of infections are primary goals of treatment of children and adults with primary immune deficiencies due to decreased antibody production. Approaches to these goals include immunoglobulin replacement therapy, vaccination, and prophylactic treatment with antimicrobials. In this review, the infectious and non-infectious complications of antibody deficiencies will be discussed along with the limited number of studies that support the effective use of the available therapies and to drive the development of new therapies. Some illustrative case studies will be presented and the outlook for additional controlled clinical trials and potential for therapies driven by the underlying disease genetics will be considered.

预防和治疗感染是治疗因抗体生成减少而出现原发性免疫缺陷的儿童和成人的主要目标。实现这些目标的方法包括免疫球蛋白替代疗法、疫苗接种和抗菌药物预防性治疗。本综述将讨论抗体缺乏症的感染性和非感染性并发症,以及支持有效使用现有疗法和推动新疗法开发的数量有限的研究。本综述将介绍一些说明性病例研究,并探讨更多临床对照试验的前景以及由潜在疾病遗传学驱动的疗法的潜力。
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引用次数: 0
Increased IFN-β indicates better survival in hepatocellular carcinoma treated with radiotherapy. 肝细胞癌放疗后 IFN-β 增高意味着生存率提高
IF 3.4 3区 医学 Q3 IMMUNOLOGY Pub Date : 2024-10-16 DOI: 10.1093/cei/uxae075
Yang Zhang, Weifeng Hong, Danxue Zheng, Zongjuan Li, Yong Hu, Yixing Chen, Ping Yang, Zhaochong Zeng, Shisuo Du

Preclinical data suggest that type I interferon (IFN) responsiveness is essential for the antitumor effects of radiotherapy (RT). However, its clinical value remains unclear. This study aimed to explore this from a clinical perspective. In cohort 1, data from 152 hepatocellular carcinoma (HCC) patients who received RT were analyzed. Blood samples were taken 1 day before and 2 weeks after RT. RT was found to increase serum levels of IFN-β (a subtype of IFN-I) in HCC patients (3.42 ± 1.57 to 5.51 ± 2.11 pg/ml, P < 0.01), particularly in those with favorable responses. Higher post-RT serum IFN-β levels (≥4.77 pg/ml) were associated with better progression-free survival (HR = 0.58, P < 0.01). Cohort 2 included 46 HCC patients, including 23 who underwent preoperative RT and 23 matched control HCC who received surgical resection without RT. Formalin-fixed paraffin-embedded samples were obtained. Neoadjuvant RT significantly increased IFN-β expression in tumor tissues compared to direct surgery (8.13% ± 5.19% to 15.10% ± 5.89%, P < 0.01). Higher post-RT IFN-β (>median) indicated better disease-free survival (P = 0.049). Additionally, increased CD11c+MHCII+CD141+ antigen-presenting cell subsets and CD103+CD39+CD8+ tumor-infiltrating lymphocytes were found in the higher IFN-β group (P = 0.02, P = 0.03), which may contribute to the favorable prognosis in higher IFN-β group. Collectively, these findings suggest that IFN-β response activated by radiation may serve as a prognostic biomarker for HCC patients undergoing RT.

临床前数据表明,I型干扰素(IFN)的反应性对放射治疗(RT)的抗肿瘤效果至关重要。然而,其临床价值仍不明确。本研究旨在从临床角度探讨这一问题。在队列 1 中,分析了 152 名接受过 RT 的肝细胞癌(HCC)患者的数据。分别在 RT 前一天和 RT 后两周采集血样。研究发现,RT 可提高 HCC 患者血清中 IFN-β(IFN-I 的一种亚型)的水平(3.42 ± 1.57 至 5.51 ± 2.11 pg/mL,p < 0.01),尤其是在那些反应良好的患者中。RT后较高的血清IFN-β水平(≥ 4.77 pg/mL)与较好的无进展生存期相关(HR = 0.58,p < 0.01)。队列 2 包括 46 例 HCC 患者,其中 23 例在术前接受了 RT 治疗,23 例与之匹配的对照组 HCC 患者在未接受 RT 治疗的情况下接受了手术切除。研究人员采集了福尔马林固定石蜡包埋样本。与直接手术相比,新辅助 RT 能显著增加肿瘤组织中 IFN-β 的表达(8.13% ± 5.19% 到 15.10% ± 5.89%,P < 0.01)。RT后IFN-β(>中位数)越高,表明无病生存率越高(p = 0.049)。此外,高IFN-β组中CD11c+MHCII+CD141+抗原递呈细胞亚群和CD103+CD39+CD8+肿瘤浸润淋巴细胞增多(p = 0.02,p = 0.03),这可能是高IFN-β组预后良好的原因。总之,这些研究结果表明,辐射激活的IFN-β反应可作为接受RT治疗的HCC患者的预后生物标志物。
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引用次数: 0
Prevalence and clinical significance of anti-SSA antibody in the Chinese health screening population. 中国健康体检人群中抗 SSA 抗体的流行率和临床意义。
IF 3.4 3区 医学 Q3 IMMUNOLOGY Pub Date : 2024-10-16 DOI: 10.1093/cei/uxae073
Yimeng Jia, Shuqi Luan, Sicheng Huang, Wen Zhang, Mengtao Li, Tengda Xu, Yunyun Fei

Anti-Sjögren's syndrome type A (anti-SSA) antibodies are non-organ-specific autoantibodies highly prevalent in various autoimmune diseases. This study primarily investigated the prevalence of anti-SSA antibodies in the health screening population. Additionally, we explored the clinical features of the anti-SSA antibody-positive population and evaluated the development of connective tissue diseases (CTD) over the years in individuals with anti-SSA antibodies for whom follow-up was available. A total of, 64 045 individuals without a history of CTD from 2013 to 2022 who visited Peking Union Medical College Hospital for health screening were screened for autoimmune antibodies: 1.7% (1091/64 045) of the Chinese health screening population were positive for anti-SSA antibodies, with a prevalence of 0.9% (290/33 829) in men and 2.7% (801/30 216) in women. Compared with matched autoantibody-negative controls, anti-SSA antibody-positive individuals had higher levels of serological abnormalities, including erythrocyte sedimentation rate (ESR) [10 (6-15) mm/h vs. 7 (4-12) mm/h, P < 0.0001], rheumatoid factor (RF) [7.15 (4.30-16.90) IU/ml vs. 5.00 (3.20-7.90) IU/ml, P < 0.0001], and immunoglobulin G [13.09 (11.20-15.45) g/L vs. 11.34 (9.85-13.18) g/L, P < 0.0001], and lower levels of white blood cells (WBC; 5.49 ± 1.50 × 109/L vs. 5.82 ± 1.49 × 109/L, P < 0.0001). Additionally, they had a higher proportion of coexisting thyroid autoantibodies, including anti-thyroid peroxidase antibodies (TPO-Ab) (17.1% vs. 11.3%, P < 0.0001) and anti-thyroglobulin antibodies (Tg-Ab) (17.8% vs. 11.0%, P < 0.0001). Among the 381 subjects who were anti-SSA positive and followed up for a median of 4.6 years, 146 (38.3%) individuals developed CTD, including 68 (17.8%) cases of primary Sjögren's syndrome (pSS), 10 (2.6%) cases of rheumatoid arthritis (RA), 5 (1.3%) cases of systemic lupus erythematosus (SLE), 4 (1.0%) cases of secondary Sjögren's syndrome (sSS), and 59 (15.5%) cases of undifferentiated connective tissue disease (UCTD). In all, 235 (61.7%) individuals did not develop CTD over a median time of 5.9 (2.9-8.1) years after the earliest autoantibody detection. Elevated ESR (>20 mm/h), RF positivity (>20 IU/ml), and female gender were identified as independent risk factors for CTD among the anti-SSA antibody-positive individuals. Anti-SSA antibodies were found in 17 among approximately 1000 individuals without a history of autoimmune diseases. Anti-SSA antibody-positive individuals are advised to periodically monitor thyroid function. Elevated ESR (>20 mm/h), female gender, and RF positivity may delineate a high-risk cohort for CTDs.

抗SSA抗体是一种非器官特异性自身抗体,在各种自身免疫性疾病中非常普遍。本研究主要调查健康检查人群中抗 SSA 抗体的流行情况。此外,我们还探讨了抗-SSA抗体阳性人群的临床特征,并评估了有随访记录的抗-SSA抗体阳性者多年来结缔组织疾病(CTD)的发展情况。对2013年至2022年期间到北京协和医院进行健康检查的64045名无CTD病史者进行了自身免疫抗体筛查。在中国健康筛查人群中,1.7%(1091/64045)的人抗SSA抗体呈阳性,男性患病率为0.9%(290/33829),女性患病率为2.7%(801/30216)。与匹配的自身抗体阴性对照组相比,抗SSA抗体阳性者的血清学异常水平更高,包括红细胞沉降率(ESR)[10 (6-15) mm/h vs. 7 (4-12) mm/h, p20 mm/h]、RF阳性(>20 IU/ml)和女性性别被认为是抗SSA抗体阳性者患CTD的独立危险因素。在大约 1000 名无自身免疫性疾病史的人中,有 17 人发现了抗-SSA 抗体。建议抗SSA抗体阳性者定期监测甲状腺功能。血沉增快(>20 mm/h)、女性和类风湿因子阳性者可能是CTD的高危人群。
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Clinical and experimental immunology
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