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Dietary lipid sources for growing rabbits: A review on animal health and productivity 生长兔饲粮脂质来源:动物健康与生产研究进展
Pub Date : 2023-01-01 DOI: 10.1590/1809-6891v24e-75704e
Alexander Alexandre de Almeida, Jean Kaique Valentim, Aline Cristina Diniz Silva, Debora Duarte Moraleco, Joyce Zanella, Leonardo da Silva Fonseca
Abstract Studies that explore the use of lipids and their effects on animal nutrition have become increasingly abundant, producing a mass of information. The review was carried out on a parity basis through a survey of articles in the bibliographic databases: Web of Science and Periodicals Capes, using search terms associated or not, in the plural or singular, in English and Portuguese, such as: “lipids” AND “rabbits" AND "nutrition". After analyzing the files in the two scientific databases, certain files were excluded because they did not fit the theme or because they did not meet the inclusion criteria and repeated articles. A relevance test was carried out for the use of the articles, where they should deal with the subject: articles that report the use of lipids in the nutrition of non-ruminant animals; articles made available in complete form; articles that had at least one of the keywords; articles where the main subject is related to lipids. The articles were selected and tabulated in an Excel® spreadsheet with relevant information for exploration in the review. The use of oils and fats is a favorable point in the nutrition of non-ruminant animals, presenting benefits in the enrichment of final products such as: providing higher levels of omega-3 and omega-6, and thus obtaining meat products with lower levels of saturated fat and higher unsaturated fat contents promoting benefits to human health through its consumption, reduction of food costs, improvement in palatability and appearance of foods. These are nutritional strategies used in hot seasons of the year due to the low calorific increment produced.
探讨脂质利用及其对动物营养影响的研究越来越多,产生了大量的信息。通过对文献数据库:Web of Science and Periodicals Capes中的文章的调查,以均等的基础进行了审查,使用相关或不相关的搜索词,以复数或单数形式,以英语和葡萄牙语进行,例如:“脂质”和“兔子”和“营养”。在对两个科学数据库中的文件进行分析后,某些文件因不符合主题或不符合纳入标准和重复文章而被排除。对文章的使用进行了相关性测试,它们应该处理以下主题:报告非反刍动物营养中脂类使用的文章;以完整形式提供的物品;至少包含其中一个关键词的文章;主要主题与脂类有关的文章。选择文章并将相关信息制成Excel®电子表格,以便在综述中进行探索。油和脂肪的使用是非反刍动物营养的一个有利点,在最终产品的丰富方面具有好处,例如:提供更高水平的omega-3和omega-6,从而获得饱和脂肪含量较低而不饱和脂肪含量较高的肉制品,通过其消费促进对人类健康的益处,降低食品成本,改善食品的可口性和外观。这些是在炎热季节使用的营养策略,因为产生的热量增量很低。
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引用次数: 0
Diseases with neurological manifestations diagnosed in cattle in the hospital routine: a retrospective epidemiological study (2009-2019) 2009-2019年医院常规诊断牛神经系统疾病回顾性流行病学研究
Pub Date : 2023-01-01 DOI: 10.1590/1809-6891v24e-76190e
Maria Isabel Souza, Gliere Silmara Leite Soares, Ângela Imperiano da Conceição, Jean Carlos Ramos Silva, Antônio Flávio Medeiros Dantas, Jobson Filipe de Paula Cajueiro, Nivaldo Azevedo Costa, Carla Lopes de Mendonça, José Augusto Bastos Afonso
Abstract Neurological disorders in cattle comprise a group of economically relevant diseases with high occurrence and mortality. In this way, the objective was to carry out an epidemiological study of the diseases with neurological manifestations in cattle admitted (alive or dead) to the Garanhuns Bovine Clinic/Federal Rural University of Pernambuco (CBG/UFRPE) from January 2009 to December 2019. Epidemiological information was collected from the clinical records regarding the age group, rearing system, time of year, and area of origin of the animals. The diseases were grouped into categories according to their origin: toxic or toxi-infectious, viral, traumatic/physical, parasitic, metabolic degenerative, bacterial, neoplastic, and others. A total of 6103 cattle were treated during the study period, of which 604 (10.1%) were diagnosed with diseases that had neurological manifestations. Of these, 331 cases were of single occurrence, and 231 cases occurred as outbreaks in the herd. Death was the outcome in almost 80% of the cases (465/596). The frequencies of the different categories were toxic or toxi-infectious (25.2%), viral (21.5%), traumatic/physical (13.6%), parasitic (9.8%), metabolic (9.3%), degenerative (2.3%), bacterial (2.2%), neoplastic (1.2%), and others (1.2%). It was found that almost half of the animals were older than 24 months (229/475), almost 80% were females, more than 60% were submitted to a semi-intensive to intensive rearing system, and the occurrence of disease was higher during the dry period of the year (363/614). The three most frequent neurological diseases in this study were rabies, trauma, and botulism.
牛的神经系统疾病是一组高发病率和高死亡率的经济相关疾病。通过这种方式,目标是在2009年1月至2019年12月期间,对伯南布哥联邦农村大学Garanhuns牛诊所(CBG/UFRPE)收治的牛(活牛或死牛)中出现神经系统症状的疾病进行流行病学研究。从临床记录中收集动物的年龄组、饲养制度、季节和原产地区的流行病学信息。这些疾病根据其来源分为:毒性或毒性感染性、病毒性、创伤性/物理性、寄生性、代谢性退行性、细菌性、肿瘤性和其他。在研究期间,共治疗了6103头牛,其中604头(10.1%)被诊断患有神经系统疾病。其中,331例为单次发病,231例为在畜群中暴发。几乎80%的病例以死亡告终(465/596)。不同类型的频率分别为毒性或毒性感染性(25.2%)、病毒性(21.5%)、创伤/物理(13.6%)、寄生虫(9.8%)、代谢性(9.3%)、退行性(2.3%)、细菌性(2.2%)、肿瘤性(1.2%)和其他(1.2%)。结果发现,年龄在24月龄以上的动物几乎占一半(229/475),雌性动物几乎占80%,半集约化至集约化饲养方式占60%以上,在一年中的旱季发病率较高(363/614)。本研究中最常见的三种神经系统疾病是狂犬病、创伤和肉毒杆菌中毒。
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引用次数: 0
Infecção multissistêmica por Dipetalonema spp. em um macaco-prego (Sapajus nigritus) 卷尾猴(Sapajus nigritus)双爪线虫多系统感染
Pub Date : 2023-01-01 DOI: 10.1590/1809-6891v24e-75264p
Bibiana da Rosa Pereira, Ezequiel Davi dos Santos, Aline Bielak dos Santos, Paula Michelson Vanzin, Bruno Webber Klaser, Marina Gatto, Sabrina Pavan Flores, Tanise Policarpo Machado, Michelli Westphal Ataíde, Maria Isabel Botelho Vieira, Adriana Costa da Motta
Resumo O macaco-prego (Sapajus nigritus) é um dos primatas que compõem a fauna silvestre do Rio Grande do Sul. Assim como outros animais silvestres, os primatas padecem de doenças infecciosas veiculadas por insetos, entre as quais, as filarioses. Este relato apresenta os aspectos anatomopatológicos de uma infecção multissistêmica por Dipetalonema spp. em um macaco-prego, fêmea, adulta, de origem silvestre, resgatado junto a uma rodovia do Norte do Estado do Rio Grande do Sul pelo serviço de policiamento rodoviário e conduzida para atendimento. Na avaliação clínica observaram-se nistagmo e movimentos involuntários da cabeça, estado semi-comatoso, hipertermia (38,7ºC), hipovolemia, múltiplas escoriações e lacerações cutâneas profundas abrangendo camadas musculares. O paciente foi hospitalizado e medicado, mas apresentou piora do quadro clínico, que evoluiu a óbito. Na necropsia, os principais achados foram na cavidade peritoneal, onde observaram-se acúmulo de líquido seroso amarelado, marcada formação de aderências fibrinosas e numerosos parasitas nematódeos livres na cavidade. Histologicamente foram observadas microfilárias no lúmen de vasos sanguíneos do pulmão, fígado, coração, rins, baço, intestinos delgado e grosso, e cérebro. Também foram constatadas peritonite, pneumonia e bronquiolite associadas à nematódeos, danos hepáticos e microgliose cerebral com satelitose, neuronofagia e malácia hemorrágica. Microfilarias e filarídeos adultos foram submetidos à identificação morfométrica e caracterizados como pertencentes ao gênero Dipetalonema. Assim, foi concluído tratar-se de um caso de infecção multissistêmica por Dipetalonema spp.
卷尾猴(Sapajus nigritus)是组成大南州野生动物的灵长类动物之一。和其他野生动物一样,灵长类动物也患有由昆虫传播的传染病,包括丝虫病。本报告介绍了一只野生卷尾猴(雌卷尾猴、成年卷尾猴)多系统双爪线虫感染的解剖病理方面,该卷尾猴在大南州北部的一条高速公路旁被公路警察救起并进行了治疗。在临床评估中,观察到眼球震颤和不自主的头部运动,半昏迷状态,热(38.7ºC),低血容量,多重擦伤和覆盖肌肉层的深层皮肤撕裂。患者住院治疗,但临床表现恶化,最终死亡。在尸检中,主要发现在腹腔,在腹腔中观察到黄色浆液的积累,明显形成纤维蛋白粘连和大量游离线虫寄生虫。组织学上观察到肺、肝、心、肾、脾、小肠、粗肠和大脑血管腔内的微丝虫。此外,还观察到与线虫相关的腹膜炎、肺炎和毛细支气管炎、肝损伤和脑小胶质增生伴卫星病、神经食管炎和出血性软化。对微丝虫和成虫进行形态学鉴定,并将其归为双翅目属。因此,我们得出结论,这是一例双花瓣菌的多系统感染。
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引用次数: 0
Fontes lipídicas na dieta de coelhos em crescimento: uma revisão sobre a saúde e a produtividade animal 生长兔饲粮中的脂质来源:动物健康和生产力综述
Pub Date : 2023-01-01 DOI: 10.1590/1809-6891v24e-75704p
Alexander Alexandre de Almeida, Jean Kaique Valentim, Aline Cristina Diniz Silva, Debora Duarte Moraleco, Joyce Zanella, Leonardo da Silva Fonseca
Resumo Estudos que exploram o uso de lipídios e seus efeitos na nutrição animal têm se tornado cada vez mais abundantes, produzindo uma grande quantidade de informações. A revisão foi realizada de forma paritária por meio de uma pesquisa de artigos nas bases bibliográficas: Web of Science e Periódicos Capes, utilizando termos de busca associados ou não, no plural ou no singular, em inglês e português, tais como: "lipídios" E "coelhos" E "nutrição". Após analisar os arquivos nas duas bases científicas, determinados arquivos foram excluídos porque não se adequavam ao tema ou não atendiam aos critérios de inclusão, além de artigos repetidos. Um teste de relevância foi realizado para a seleção dos artigos, nos quais eles deveriam tratar do assunto: artigos que relatam o uso de lipídios na nutrição de animais não-ruminantes; artigos disponibilizados na forma completa; artigos que possuíam pelo menos uma das palavras-chave; artigos nos quais o assunto principal está relacionado a lipídios. Os artigos foram selecionados e tabulados em uma planilha do Excel® com informações relevantes para exploração na revisão. O uso de óleos e gorduras é um ponto favorável na nutrição de animais não-ruminantes, apresentando benefícios no enriquecimento de produtos finais, tais como: fornecer níveis mais altos de ômega-3 e ômega-6, e assim obter produtos de carne com menores teores de gordura saturada e maiores teores de gordura insaturada, promovendo benefícios para a saúde humana por meio do consumo, redução dos custos alimentares, melhoria na palatabilidade e aparência dos alimentos. Essas são estratégias nutricionais usadas nas estações quentes do ano devido ao baixo incremento calórico produzido.
探索脂类的使用及其对动物营养的影响的研究越来越多,产生了大量的信息。该综述是通过对书目数据库:Web of Science和Capes期刊上的文章进行对等搜索进行的,使用英语和葡萄牙语中复数或单数相关或不相关的搜索词,如“lipidios”、“coelhos”和“nutrition”。在对两个科学基地的档案进行分析后,某些档案因不适合主题或不符合纳入标准而被排除,以及重复的文章。对应处理该主题的文章进行了相关性测试:报道非反刍动物营养中脂质使用的文章;以完整形式提供的文章;至少有一个关键词的文章;主要主题与脂类有关的文章。文章被选择并制成表格,在Excel®电子表格中包含相关信息,以便在审查中进行探索。油脂的使用是有利的在动物营养不-ruminantes,特权在浓缩的最终产品,如:提供较高的ω- 3和ω- 6,所以得到肉制品和低水平的饱和脂肪和高水平的不饱和脂肪,促进有利于人类健康的食品消费环境,降低成本,改善食品风味和外观。这些营养策略在温暖的季节使用,因为产生的热量增加很低。
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引用次数: 0
Diagnósticos histomorfológico e imunofenotípico de tumores estromais gastrointestinais e outros sarcomas que acometem o intestino de cães 胃肠道间质瘤和其他影响犬肠道的肉瘤的组织形态学和免疫表型诊断
Pub Date : 2023-01-01 DOI: 10.1590/1809-6891v24e-75610p
Marina Laudares Costa, Felipe Augusto Ruiz Sueiro, Paulo César Jark, Leonardo Lamarca de Carvalho, Marcela Aldrovani Rodrigues, Vinícius Thomaz da Silva Almeida, Fernanda Gosuen Gonçalves Dias
Resumo Tendo em vista a semelhança morfológica entre tumores estromais gastrointestinais (GIST) e outros sarcomas do intestino de cães, objetivou-se realizar o diagnóstico histomorfológico e imunoistoquímico desses tumores, associando raça, sexo e idade, localização e invasão tumoral. Foram avaliados 217 casos por histopatologia e incluídos 36 diagnosticados por imuno-histoquímica (24 GIST e 12 outros sarcomas intestinais). Cães sem raça definida foram os mais diagnosticados com GIST, principalmente fêmeas idosas (9,5±2,2 anos); nos demais sarcomas intestinais, mestiços e Dachshunds, machos e fêmeas, foram igualmente acometidos. O ceco foi o mais acometido pelos GISTs, com invasão tumoral das camadas intestinais em todos os casos. O intestino delgado foi o mais acometido pelos demais sarcomas intestinais, com invasão das camadas na maioria desses tumores. GISTs expressaram marcadores como CD117 e DOG-1, ao contrário de outros sarcomas intestinais. O GIST e outros sarcomas intestinais denotaram características histomorfológicas e imunofenotípicas semelhantes à histopatologia, justificando a associação da imuno-histoquímica para o diagnóstico definitivo.
摘要鉴于犬胃肠道间质瘤(GIST)与其他犬肠肉瘤在形态学上的相似性,本研究旨在对犬肠间质瘤进行组织形态学和免疫组化诊断,并结合犬种、性别、年龄、肿瘤位置和侵袭性进行诊断。我们评估了217例组织病理学病例,包括36例免疫组化诊断的病例(24例GIST和12例其他肠道肉瘤)。未确定品种的狗被诊断为GIST最多,主要是老年雌性(9.5±2.2岁);在其他肠道肉瘤中,雄性和雌性的杂交和腊肠肉瘤也受到影响。盲肠是胃肠道感染最严重的部位,所有病例均伴有肠层肿瘤侵袭。小肠是受其他肠道肉瘤影响最大的肿瘤,在大多数这些肿瘤中都有侵袭层。与其他肠道肉瘤不同,gist表达CD117和DOG-1等标记物。GIST和其他肠道肉瘤表现出与组织病理学相似的组织形态学和免疫表型特征,证明免疫组化对明确诊断的相关性。
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引用次数: 0
Fuzzy logic discriminant function for evaluating goats exposed to verminosis occurrence regarding resistance, resilience, or sensitivity to parasitism 用于评价暴露于寄生虫病发生的山羊对寄生虫的抗性、恢复力或敏感性的模糊逻辑判别函数
Pub Date : 2023-01-01 DOI: 10.1590/1809-6891v24e-74727e
Wellhington Paulo da Silva Oliveira, Natanael Pereira da Silva Santos, Max Brandão de Oliveira, Amauri Felipe Evangelista, Raimundo Tomaz da Costa Filho, Adriana Mello de Araújo
Abstract Worm infections pose a significant challenge to goat farming in the tropics. While individual variations in the animals' response to this disease are observed, understanding its genetic component is crucial for establishing effective herd production management, prioritizing the selection of goats with higher resistance to parasitism. This study aimed to assess goat response to worm infection under natural field conditions using data on eggs per gram of feces (EPG), body condition score (BCS), and conjunctival mucosa coloration (FAMACHA©). Cluster analysis and artificial intelligence (AI) techniques were applied to 3,839 data points from 200 individuals in an experimental goat herd in Piauí, Brazil. The study considered the phenotypic expression of resistance, sensitivity, and resilience to worm infection as responses to parasitism. Three clustering methods, namely Ward, Average, and k-means, were employed and compared with fuzzy logic obtained through the CAPRIOVI web software. The analysis revealed statistically significant differences (P<0.05) between the groups of animals classified as resistant, resilient, and sensitive to parasitism. Pregnancy and peripartum were identified as stages of heightened sensitivity to parasitism (P<0.05). Among the clustering techniques, traditional statistical methods exhibited excellent performance, with an overall accuracy percentage exceeding 90.00%. In contrast, CAPRIOVI's fuzzy logic demonstrated lower overall accuracy (77.00%). The clustering methods showed similar efficiency, but differed in terms of the distribution of animals per group, with a tendency towards greater numbers in the resistant category. Fuzzy logic circumvented this limitation by enabling the formation of groups tailored to meet the producer's interests, adding consistency in terms of the animals' response to worm infection. This finding highlights the potential of the software for goat health management.
蠕虫感染对热带地区的山羊养殖业构成了重大挑战。虽然观察到动物对这种疾病的反应存在个体差异,但了解其遗传成分对于建立有效的羊群生产管理至关重要,优先选择抗寄生虫能力较强的山羊。本研究旨在利用每克粪便产蛋数(EPG)、体况评分(BCS)和结膜黏膜着色(FAMACHA©)等数据,评估山羊在自然野外条件下对蠕虫感染的反应。聚类分析和人工智能(AI)技术应用于巴西Piauí实验山羊群中200只个体的3839个数据点。该研究考虑了对蠕虫感染的抗性、敏感性和恢复力的表型表达作为对寄生的反应。采用Ward、Average、k-means三种聚类方法,并与CAPRIOVI网络软件得到的模糊逻辑进行比较。分析显示,抗、抗、敏感三组动物之间存在统计学上的显著差异(P<0.05)。妊娠期和围产期被认为是对寄生虫高度敏感的阶段(P<0.05)。在聚类技术中,传统的统计方法表现优异,总体准确率超过90.00%。相比之下,CAPRIOVI模糊逻辑的总体准确率较低(77.00%)。聚类方法具有相似的效率,但在每组动物分布方面存在差异,抗性类别的数量倾向较多。模糊逻辑规避了这一限制,使群体的形成符合生产者的利益,增加了动物对蠕虫感染反应的一致性。这一发现凸显了该软件在山羊健康管理方面的潜力。
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引用次数: 0
Multisystem infection by Dipetalonema spp. in a capuchin monkey (Sapajus nigritus) 卷尾猴双爪虫多系统感染
Pub Date : 2023-01-01 DOI: 10.1590/1809-6891v24e-75264e
Bibiana da Rosa Pereira, Ezequiel Davi dos Santos, Aline Bielak dos Santos, Paula Michelson Vanzin, Bruno Webber Klaser, Marina Gatto, Sabrina Pavan Flores, Tanise Policarpo Machado, Michelli Westphal Ataíde, Maria Isabel Botelho Vieira, Adriana Costa da Motta
Abstract The capuchin monkey (Sapajus nigritus) is one of the primates that make up the wild fauna of Rio Grande do Sul. Like other wild animals, primates suffer from infectious diseases transmitted by insects, including filariasis. This report presents the anatomopathological aspects of a multisystemic infection by Dipetalonema spp. in a capuchin monkey, female, adult, of wild origin, rescued along a highway in the north of the state of Rio Grande do Sul by the road policing service and taken for assistance. In the clinical evaluation, nystagmus and involuntary head movements, semi-comatose state, hyperthermia (38.7ºC), hypovolemia, multiple abrasions and deep skin lacerations covering muscle layers were observed. The patient was hospitalized and medicated, but his clinical picture worsened, which resulted in death. At necropsy, the main findings were in the peritoneal cavity, where there was an accumulation of yellowish serous fluid, marked formation of fibrinous adhesions and numerous free nematode parasites in the cavity. Histologically, microfilariae have been observed in the lumen of blood vessels in the lung, liver, heart, kidneys, spleen, small and large intestines, and brain. Peritonitis, pneumonia and bronchiolitis associated with nematodes, liver damage and cerebral microgliosis with satellitosis, neuronophagia and hemorrhagic malacia were also observed. Adult microfilariae and filarids were submitted to morphometric identification and characterized as belonging to the genus Dipetalonema. Thus, it was concluded that this was a case of multisystemic infection by Dipetalonema spp.
卷尾猴(学名:Sapajus nigritus)是南里奥格兰德州野生动物群的灵长类动物之一。和其他野生动物一样,灵长类动物也患有由昆虫传播的传染病,包括丝虫病。本报告介绍了Dipetalonema在一只野生雌性成年卷尾猴身上的多系统感染的解剖病理学方面,该卷尾猴在南里奥格兰德州北部的一条高速公路上被道路警察救出并寻求帮助。在临床评价中,观察到眼球震颤和头部不自主运动,半昏迷状态,高热(38.7℃),低血容量,多处擦伤和覆盖肌肉层的深层皮肤撕裂。患者住院并接受了药物治疗,但他的临床症状恶化,导致死亡。尸检时,主要发现是在腹膜腔,那里有黄色浆液积聚,纤维性粘连形成明显,腔内有大量游离线虫寄生虫。组织学上,在肺、肝、心、肾、脾、小肠、大肠和脑的血管腔中均可见微丝蚴。伴有线虫的腹膜炎、肺炎、细支气管炎、肝损害、脑小胶质瘤伴卫星病、神经性噬神经症和出血性软化症。成虫微丝虫和丝虫经形态鉴定属于双足丝虫属。因此,我们认为这是一个多系统感染的双佩塔单胞菌。
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引用次数: 0
Growth and differentiation factor - 9 (GDF-9) increases the in vitro growth rates of isolated goat early antral follicles 生长分化因子-9 (Growth and differentiation factor -9, GDF-9)能提高离体山羊早期窦卵泡的体外生长速率
Pub Date : 2023-01-01 DOI: 10.1590/1809-6891v24e-74980e
André Luiz da Conceição Santos, Anna Clara Accioly Ferreira, Naiza Arcângela Ribeiro de Sá, Renato Félix da Silva, Gaby Judith Quispe Palomino, Éverton Pimentel Ferreira Lopes, Jesús Cadenas, Benner Geraldo Alves, Juliana Jales de Hollanda Celestino, Ana Paula Ribeiro Rodrigues, José Ricardo de Figueiredo
Abstract This study aimed to investigate the effect of growth and differentiation factor 9 (GDF-9) during the in vitro culture of isolated caprine early antral follicles. The isolated and selected early antral follicles were individually cultured for 18 days, and the following treatments were tested: α-MEM+ (control treatment) or α-MEM+ supplemented with 200 ng/mL GDF-9. The following endpoints were evaluated: follicular growth and morphology, estradiol production, oocyte nuclear maturation, and relative expression of key genes related to steroidogenesis (CYP19A1, CYP17, and insulin receptor) and basement membrane remodeling (MMP-9 and TIMP-2). In both treatments, a decrease was observed in the percentage of morphologically intact follicles with a concomitant increase in the rates of extruded and degenerated follicles (P < 0.05). The GDF-9 treatment showed higher rates of extruded follicles only on day 6 of culture (P < 0.05). Follicle diameter increased progressively throughout the culture period (P < 0.05) with similar diameters between treatments at all culture times (P > 0.05). Growth and differentiation factor 9 increased the daily growth rate from the first to the second third of culture, with higher values (P < 0.05) than control in the second third. Oocyte maturation rate as well as estradiol levels and relative mRNA expression for CYP19A1, CYP17, MMP-9, TIMP-2, and insulin receptor genes were similar between treatments (P > 0.05). This study shows for the first time that GDF-9 added to a culture medium increased the follicle growth rate of goat early antral follicles cultured in vitro.
摘要本研究旨在探讨生长与分化因子9 (GDF-9)在离体山羊早期窦卵泡体外培养中的作用。将分离和选择的早期窦卵泡单独培养18 d,分别进行α-MEM+(对照处理)和α-MEM+中添加200 ng/mL GDF-9的处理。评估了以下终点:卵泡生长和形态、雌二醇产生、卵母细胞核成熟以及与甾体生成(CYP19A1、CYP17和胰岛素受体)和基底膜重塑(MMP-9和TIMP-2)相关的关键基因的相对表达。在两种治疗中,观察到形态完整的卵泡百分比下降,同时挤压和变性卵泡率增加(P <0.05)。GDF-9处理仅在培养第6天显示出较高的卵泡挤出率(P <0.05)。在整个培养过程中,卵泡直径逐渐增大(P <0.05),各培养时间处理间直径相近(P >0.05)。生长和分化因子9从培养的第1 / 3到第2 / 3增加了日生长率,具有较高的值(P <0.05)。卵母细胞成熟率、雌二醇水平以及CYP19A1、CYP17、MMP-9、TIMP-2和胰岛素受体基因的相对mRNA表达量在两组间相似(P >0.05)。本研究首次发现,在培养基中添加GDF-9可提高体外培养的山羊早期腔囊的卵泡生长速度。
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引用次数: 0
Estrus response patches, timing for artificial insemination, and GnRH protocol in Split Timed AI beef cattle 分裂定时AI肉牛的发情反应贴片、人工授精时机和GnRH方案
Pub Date : 2023-01-01 DOI: 10.1590/1809-6891v24e-75115e
Danilo Demeterco, Ryon S. Walker, Luiz Ernandes Kozicki, Vitor Mohad Valle, A.K. Edwards, Jake E. Anderson, Cathleen Collet Williams
Abstract An estrous-detection patch was used to determine the optimum timing for STAI and the necessity of GnRH at STAI on a 7-day CO-Synch+CIDR protocol. Crossbred beef cows (n=216) were stratified into the following treatment groups: CTRL=TAI (n=67) at 72 h post CIDR removal, or TRT=STAI (n=149) at 72 or 84 h post CIDR removal. All females received GnRH (100 mcg) plus a CIDR on d0, PGF2α, CIDR removal, and an Estrotect estrous-detector patch on d7. At 72 h post-CIDR removal, a patch score was assigned (PS1<50% removed; PS2>50% removed) to all females. Cows in the CTRL group were administered a second GnRH (100 mcg) at 72 h TAI. Cows in the TRT group with PS2 were not administered GnRH. At 84 h, the remaining TRT group cows were given a second PS; cows with PS1 received a GnRH (100 mcg), and cows with PS2 were not administered. Results: The TAI pregnancy rates were similar (P=0.81) between the CTRL (45.6%) and TRT (44.8%) groups. Pregnancy rates tended to be higher (P=0.07) for cows with PS2 (50.3%) than for those with PS1 (29.4%). However, by extending TAI to 84 h in unresponsive cows, 82.0% of TRT cows did not receive a second injection of GnRH at TAI. It was concluded that the estrous detector patches reduced the percentage of cows that required GnRH at TAI without compromising pregnancy rates. The estrous detector patches significantly reduced the number of cows that received a second GnRH injection at TAI.
在7天co - sync +CIDR协议下,采用发情检测补丁确定STAI的最佳时机和在STAI时GnRH的必要性。将216头杂交肉牛分为以下处理组:去除CIDR后72 h,按CTRL=TAI (n=67)处理;去除CIDR后72、84 h,按TRT=STAI (n=149)处理。所有雌性小鼠在第10天接受GnRH (100 mcg)加CIDR,在第7天接受PGF2α,去除CIDR和estroprotect发情检测器贴片。在cidr移除72 h后,分配补丁评分(PS1<50%移除;PS2>50%删除)所有女性。对照组奶牛在TAI 72 h时再次注射GnRH (100 mcg)。PS2的TRT组奶牛不给GnRH。84 h,其余TRT组奶牛给予第二次PS;PS1奶牛注射GnRH (100 mcg), PS2奶牛不注射GnRH。结果:对照组(45.6%)与对照组(44.8%)TAI妊娠率差异无统计学意义(P=0.81)。PS2奶牛的妊娠率(50.3%)高于PS1奶牛(29.4%)(P=0.07)。然而,通过将无反应奶牛的TAI延长至84 h, 82.0%的TRT奶牛在TAI时未接受第二次GnRH注射。综上所述,发情检测贴片在不影响妊娠率的情况下降低了妊娠期奶牛GnRH需要量的百分比。发情检测补丁显著减少了在TAI接受第二次GnRH注射的奶牛数量。
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引用次数: 0
Resposta do adesivo marcador do estro, inseminação artificial em tempo fixo e protocolo de GnRH em bovinos de corte na Inseminação artificial dividida 分体人工授精肉牛发情标记、固定时间人工授精和GnRH方案的反应
Pub Date : 2023-01-01 DOI: 10.1590/1809-6891v24e-75115p
Danilo Demeterco, Ryon S. Walker, Luiz Ernandes Kozicki, Vitor Mohad Valle, A.K. Edwards, Jake E. Anderson, Cathleen Collet Williams
Resumo Um adesivo de detecção de estro foi usado como ferramenta para determinar o momento ideal para a inseminação artificial em tempo dividido (STAI) e a necessidade de injeção de hormônio liberador de gonadotrofinas (GnRH) no 7º dia do protocolo CO-Sinc + CIDR sem comprometer as taxas de prenhez. As vacas eram cruzadas, multíparas e lactantes (Angus x Charolês, n=216) e foram estratificadas por idades (5,9 2.5 anos), BW (581 67kg), BCS (5,3 0,8; 1-9), intervalo entre partos (78,5 15,5 dias). O grupo de tratamento CTRL = IAT (n=67) foi inseminado após 72 h após a remoção do CIDR; já no grupo tratamento TRT= IATP (n=149) as vacas foram inseminadas 72 ou 84 h após a remoção do CIDR. Todas as fêmeas receberam GnRH (100 mcg I.M.), mais um CIDR (1,38 g de progesterone) no D0, no D7 foi realizado a retirada do CIDR, aplicação de PGF2α (25 mcg I.M.) e colocação do adesivo detector de cio. Após 72 h da remoção do CIDR, uma pontuação foi atribuída ao adesivo (OS1<50% removido; OS2> 50% removido) de todas as fêmeas. As vacas do grupo CTRL receberam a segunda injeção de GnRH (100 mcg I.M.) às 72 h na IAT. Vacas do grupo TRT com OS2 não receberam GnRH. Às 84 h as vacas restantes do grupo TRT receberam um segundo OS, aquelas com OS1 receberam GnRH (100 mcg I.M.) e as vacas com OS2 não receberam GnRH. Amostras de sangue para concentração de progesterona foram coletadas nos D-11 e D-0 para determinar o percentual de vacas ciclando. Os dados foram analisados utilizando-se o Proc Genmod, tendo o tratamento e o técnico de IA como efeitos fixos, o touro como efeito aleatório e o BW, BCS, idade e IBP como covariáveis. Resultados: as taxas de gravidez da IAT foram semelhantes (P= 0,81) entre os grupos CTRL (45,6%) e TRT (44,8%). As taxas de prenhez tenderam a ser maiores (P=0,07) para vacas com OS2 (50,3%) do que para aquelas com OS1 (29,4%). No entanto, ao estender a IAT para 84 horas em vacas não responsivas, 82,0% das vacas TRT não receberam uma segunda aplicação de GnRH na IAT. Concluiu-se que os adesivos detectores de estro reduziram a porcentagem de vacas que necessitaram de GnRH na IAT sem comprometer as taxas de prenhez. Os adesivos de detecção de estro reduziram significativamente o número de vacas que receberam uma segunda injeção de GnRH na IAT.
摘要采用发情检测贴片作为工具,在不影响妊娠率的情况下,确定分时人工授精(STAI)的最佳时间和在CO-Sinc + CIDR方案第7天注射促性腺激素释放激素(GnRH)的需要。采用杂交、经产和哺乳期奶牛(Angus x charoles, n=216),按年龄(5.9 2.5岁)、BW (581 67kg)、BCS (5.3 0.8 kg)、BCS (5.9 2.5 kg)和BCS (5.9 2.5 kg)进行分层。1-9),分娩间隔(78.5 - 15.5天)。治疗组CTRL = IAT (n=67)在移除CIDR 72 h后人工授精;处理组TRT= IATP (n=149)在去除CIDR后72或84 h进行授精。所有女性在第0天接受GnRH (100 mcg I.M.)和CIDR (1.38 g孕酮),第7天取出CIDR,应用PGF2α (25 mcg I.M.)并放置cio检测贴片。去除CIDR 72小时后,对粘合剂进行评分(os1 < 50%去除)。OS2>50%移除)所有雌性。CTRL组奶牛在IAT 72 h第二次注射GnRH (100 mcg I.M.)。TRT组OS2奶牛未接受GnRH治疗。84 h时,TRT组其余奶牛接受第二次so, OS1奶牛接受GnRH (100 mcg I.M.), OS2奶牛不接受GnRH。在D-11和D-0采集血液中孕酮浓度的样本,以确定循环奶牛的百分比。数据采用Genmod程序进行分析,治疗和人工智能技术为固定效应,公牛为随机效应,BW、BCS、年龄和IBP为协变量。结果:CTRL组(45.6%)和TRT组(44.8%)的IAT妊娠率相似(P= 0.81)。OS2奶牛(50.3%)的怀孕率往往高于OS1奶牛(29.4%)。然而,当无反应奶牛延长tai至84小时时,82.0%的TRT奶牛没有在tai中第二次应用GnRH。我们的结论是,发情检测贴片在不影响怀孕率的情况下降低了tai中需要GnRH的奶牛的百分比。发情检测贴片显著减少了在tai中第二次注射GnRH的奶牛数量。
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引用次数: 0
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Ciência Animal Brasileira
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