In isolated intestine of the European Flounder, cyclic AMP and theophylline induce a reduction of the negative transmural potential difference and short circuit current, increase severalfold the passive permeability for chloride and abolish the active transport of this ion. These effects are very different from those described in mammals.
{"title":"[Cyclic AMP and control of ion exchanges in the fish intestine].","authors":"W C Mackay, B Lahlou, J Prothé-Nibelle","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>In isolated intestine of the European Flounder, cyclic AMP and theophylline induce a reduction of the negative transmural potential difference and short circuit current, increase severalfold the passive permeability for chloride and abolish the active transport of this ion. These effects are very different from those described in mammals.</p>","PeriodicalId":10544,"journal":{"name":"Comptes rendus hebdomadaires des seances de l'Academie des sciences. Serie D: Sciences naturelles","volume":"287 13","pages":"1239-42"},"PeriodicalIF":0.0,"publicationDate":"1978-11-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"11432629","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
In 5 French Alpine goats, omental adipose tissue acetyl-CoA carboxylase, glucose-6-phosphate deshydrogenase, malic enzyme and lipoprotein lipase activities significantly decreased during the third month of gestation, whereas plasma non-esterified fatty acid and triacyglycerol contents increased. This probably reflects an early decreasing rate of adipose tissue anabolism during gestation in the Goat. At the third week of lactation, anabolic activities relative to DNA content of adipose tissue were extremely low, and the tissue weight relative to DNA was lower than during gestation. Metabolic alterations of omental adipose tissue in early lactation do not seem to be related to milk production level. These results could contribute to a better control of the kinetic of body lipid stores during the reproductive cycle in high milk yielding ruminants.
{"title":"[Metabolic activity of adipose tissue in the goat during gestation and at the beginning of lactation].","authors":"Y Chilliard, G Durand, D Sauvant, P Morand-Fehr","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>In 5 French Alpine goats, omental adipose tissue acetyl-CoA carboxylase, glucose-6-phosphate deshydrogenase, malic enzyme and lipoprotein lipase activities significantly decreased during the third month of gestation, whereas plasma non-esterified fatty acid and triacyglycerol contents increased. This probably reflects an early decreasing rate of adipose tissue anabolism during gestation in the Goat. At the third week of lactation, anabolic activities relative to DNA content of adipose tissue were extremely low, and the tissue weight relative to DNA was lower than during gestation. Metabolic alterations of omental adipose tissue in early lactation do not seem to be related to milk production level. These results could contribute to a better control of the kinetic of body lipid stores during the reproductive cycle in high milk yielding ruminants.</p>","PeriodicalId":10544,"journal":{"name":"Comptes rendus hebdomadaires des seances de l'Academie des sciences. Serie D: Sciences naturelles","volume":"287 12","pages":"1131-4"},"PeriodicalIF":0.0,"publicationDate":"1978-11-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"11260550","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
We have developed a radio-enzymatic assay for systemic catecholamines in the frog. Such are its specificity and sensibility that adrenaline and noradrenaline may be measured in 50 microliter of plasma samples, the withdrawal of which strongly influenced the results. The smaller values were obtained in plasma withdrawn from canulated animals. In this case, adrenaline was the major catecholamine in the plasma: 190 +/- 55 ng/100 ml versus 35 +/- 18 ng/100 ml for noradrenaline.
{"title":"[Estimation of systemic catecholamine levels in the frog, using the technic of radio-enzymatic assay].","authors":"P Bourgeois, W Dupont, R Vaillant","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>We have developed a radio-enzymatic assay for systemic catecholamines in the frog. Such are its specificity and sensibility that adrenaline and noradrenaline may be measured in 50 microliter of plasma samples, the withdrawal of which strongly influenced the results. The smaller values were obtained in plasma withdrawn from canulated animals. In this case, adrenaline was the major catecholamine in the plasma: 190 +/- 55 ng/100 ml versus 35 +/- 18 ng/100 ml for noradrenaline.</p>","PeriodicalId":10544,"journal":{"name":"Comptes rendus hebdomadaires des seances de l'Academie des sciences. Serie D: Sciences naturelles","volume":"287 12","pages":"1149-52"},"PeriodicalIF":0.0,"publicationDate":"1978-11-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"11332032","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
F Petitjean, C Buda, M Janin, M Touret, D Salvert, M Jouvet, P Bobillier
In normal cats 90 min. after the intravenous injection of 5 mg/kg of 5 HTP and 3H 5 HTP or DOPA and 3H DOPA a few reactive nerve cell bodies were identified by radioautography. On the contrary, 48 hrs. after p-chlorophenylalanin or subtotal lesion of raphe nuclei, these tracers showed numerous reactive neurons inside or outside the central monoaminergic systems.
{"title":"[Sleep and monoamines: differential radioautography of central neurons after systematic injection of tritiated 5-hydroxytryptophane (5 HTP 3H) or of dihydroxyphenylalanine (DOPA 3H)].","authors":"F Petitjean, C Buda, M Janin, M Touret, D Salvert, M Jouvet, P Bobillier","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>In normal cats 90 min. after the intravenous injection of 5 mg/kg of 5 HTP and 3H 5 HTP or DOPA and 3H DOPA a few reactive nerve cell bodies were identified by radioautography. On the contrary, 48 hrs. after p-chlorophenylalanin or subtotal lesion of raphe nuclei, these tracers showed numerous reactive neurons inside or outside the central monoaminergic systems.</p>","PeriodicalId":10544,"journal":{"name":"Comptes rendus hebdomadaires des seances de l'Academie des sciences. Serie D: Sciences naturelles","volume":"287 12","pages":"1135-9"},"PeriodicalIF":0.0,"publicationDate":"1978-11-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"11375159","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The action of sinusoidal stretching at 1,5 Hz on primary ending frequencygrams due to repetitive stimulation of static gamma axons has been studied, in nembutalized cats, on flexor hallucis longus muscle spindles. Whenever, in static conditions, driving is elicited by the repetitive stimulation of gamma axons, the elementary components of the frequencygrams increase in amplitude during muscle stretch. This effect is not observed when the stimulation of the gamma axons gives a regular acceleration of the primary endings without "driving".
{"title":"[Heterogeneous effects of sinusoidal muscular stretching on frequency grams of primary endings due to stimulation of static gamma axons].","authors":"F Emonet-Dénand, Y Laporte","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The action of sinusoidal stretching at 1,5 Hz on primary ending frequencygrams due to repetitive stimulation of static gamma axons has been studied, in nembutalized cats, on flexor hallucis longus muscle spindles. Whenever, in static conditions, driving is elicited by the repetitive stimulation of gamma axons, the elementary components of the frequencygrams increase in amplitude during muscle stretch. This effect is not observed when the stimulation of the gamma axons gives a regular acceleration of the primary endings without \"driving\".</p>","PeriodicalId":10544,"journal":{"name":"Comptes rendus hebdomadaires des seances de l'Academie des sciences. Serie D: Sciences naturelles","volume":"287 12","pages":"1145-8"},"PeriodicalIF":0.0,"publicationDate":"1978-11-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"11332031","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"[Early organization of the dermatome and of the sclerotome in 2 anura amphibias Rana dalmatina bon. and Bufo bufo L].","authors":"J J Brustis","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":10544,"journal":{"name":"Comptes rendus hebdomadaires des seances de l'Academie des sciences. Serie D: Sciences naturelles","volume":"287 12","pages":"1153-5"},"PeriodicalIF":0.0,"publicationDate":"1978-11-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"11332033","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
It is usually accepted that macrophages "activated" by lymphokines may be found cytotoxic against tumoral target cells but show no detectable cytotoxicity in in vitro tests using normal non tumoral cells as target cells. These data have been obtained mainly with the chromium-release test. The present paper describes a new test using normal isolated pancreatic cells as target cells and evaluating the effect of activated or non-activated macrophages on the insulin secretion response to glucose stimulation. The results show a striking decrease in this response following an 18-hr incubation of pancreatic islet cells with activated macrophages, as compared to that of the same cells incubated with control macrophages. This is clear evidence that activated macrophages may alter normal cells and suggests that their cytotoxic properties are not restricted to tumoral target cells.
{"title":"[Functional inhibition of isolated pancreatic cells, new technic for the detection of macrophage cytotoxicity].","authors":"M Debray-Sachs, R Assan, D Bailey, J Hamburger","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>It is usually accepted that macrophages \"activated\" by lymphokines may be found cytotoxic against tumoral target cells but show no detectable cytotoxicity in in vitro tests using normal non tumoral cells as target cells. These data have been obtained mainly with the chromium-release test. The present paper describes a new test using normal isolated pancreatic cells as target cells and evaluating the effect of activated or non-activated macrophages on the insulin secretion response to glucose stimulation. The results show a striking decrease in this response following an 18-hr incubation of pancreatic islet cells with activated macrophages, as compared to that of the same cells incubated with control macrophages. This is clear evidence that activated macrophages may alter normal cells and suggests that their cytotoxic properties are not restricted to tumoral target cells.</p>","PeriodicalId":10544,"journal":{"name":"Comptes rendus hebdomadaires des seances de l'Academie des sciences. Serie D: Sciences naturelles","volume":"287 12","pages":"1161-4"},"PeriodicalIF":0.0,"publicationDate":"1978-11-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"11332034","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The insoluble pellet of human mammary carcinomas was solubilized by an acid buffer. Antiserum prepared with this acidosoluble fraction, after suitable absorption gave one precipitin line with the immunizing extracts: this line is different from those given by the tumor associated antigens actually known. The same antiserum reacted only with sections of human mammary carcinomas by immunofluorescence . It did not stain sections of normal mammary glands or benign mammary diseases. Reactivity with cancers of other organs was absent or doubtful. Hence it is likely that an antigen associated to human mammary carcinomas was characterized.
{"title":"[Demonstration of antigen associated with human breast cancers].","authors":"F Loisillier, D Métivier, P Burtin","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The insoluble pellet of human mammary carcinomas was solubilized by an acid buffer. Antiserum prepared with this acidosoluble fraction, after suitable absorption gave one precipitin line with the immunizing extracts: this line is different from those given by the tumor associated antigens actually known. The same antiserum reacted only with sections of human mammary carcinomas by immunofluorescence . It did not stain sections of normal mammary glands or benign mammary diseases. Reactivity with cancers of other organs was absent or doubtful. Hence it is likely that an antigen associated to human mammary carcinomas was characterized.</p>","PeriodicalId":10544,"journal":{"name":"Comptes rendus hebdomadaires des seances de l'Academie des sciences. Serie D: Sciences naturelles","volume":"287 12","pages":"1169-72"},"PeriodicalIF":0.0,"publicationDate":"1978-11-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"11309736","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
In this paper we propose a method allowing the representation of time-dependent changes in symptomatology. In algorithm, which automatically generates the sequence of stable states in patient's symptomatology evolution, describes slow, as well as fast, variations of diseases. This sequential representation of data can then be analysed by classical statistical procedures in a population of patients suffering from pancreatic diseases, to estimate the rate of global modification of clinical symptomatology in time.
{"title":"[Presentation and automatic treatment of evolutive data in medicine. Application to clinical pancreatic pathology].","authors":"M Rodriguez, R C Cros, J Cornée","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>In this paper we propose a method allowing the representation of time-dependent changes in symptomatology. In algorithm, which automatically generates the sequence of stable states in patient's symptomatology evolution, describes slow, as well as fast, variations of diseases. This sequential representation of data can then be analysed by classical statistical procedures in a population of patients suffering from pancreatic diseases, to estimate the rate of global modification of clinical symptomatology in time.</p>","PeriodicalId":10544,"journal":{"name":"Comptes rendus hebdomadaires des seances de l'Academie des sciences. Serie D: Sciences naturelles","volume":"287 12","pages":"1165-8"},"PeriodicalIF":0.0,"publicationDate":"1978-11-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"11332035","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Comparison of adjacent serial sections of the tubero-infundibular region of Human adult hypothalamus demonstrates that the same perikarya, axons and terminals are stained both with anti-beta-endorphin and anti 17-39 ACTH antisera. The most immunoreactive of these neurons are also revealed with anti alpha-endorphin, anti alpha and beta-MSH, anti-1-24 ACTH and anti beta-LPH. These results suggest that neurons of the infundibular nucleus can store and probably secrete peptide similar to propiocortin or fragment(s) of this molecule.
{"title":"[Antigenic determinants of beta-LPH, beta-MSH, alpha-endorphin, ACTH and alpha-MSH revealed by anti-beta-endorphin in neurons of the human infundibular nucleus].","authors":"B Bloch, C Bugnon, D Fellmann, D Lenys","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Comparison of adjacent serial sections of the tubero-infundibular region of Human adult hypothalamus demonstrates that the same perikarya, axons and terminals are stained both with anti-beta-endorphin and anti 17-39 ACTH antisera. The most immunoreactive of these neurons are also revealed with anti alpha-endorphin, anti alpha and beta-MSH, anti-1-24 ACTH and anti beta-LPH. These results suggest that neurons of the infundibular nucleus can store and probably secrete peptide similar to propiocortin or fragment(s) of this molecule.</p>","PeriodicalId":10544,"journal":{"name":"Comptes rendus hebdomadaires des seances de l'Academie des sciences. Serie D: Sciences naturelles","volume":"287 11","pages":"1019-22"},"PeriodicalIF":0.0,"publicationDate":"1978-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"11306206","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}