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Isolation and Identification of Pyrene Degrading Bacteria and its Pathway from Suez Oil Processing Company, Suez, Egypt. 埃及苏伊士石油加工公司产芘降解菌的分离鉴定及途径研究。
Pub Date : 2018-05-01 DOI: 10.18576/JEHE/060202
M. Abo-State, Farida M. S. E. El-Dars, Bahaaeldin A. Abdin
An indigenous bacterial isolate MAM-P39 was isolated from petroleum polluted soil by selective enrichment with pyrene as the sole source of carbon and energy. During growth on pyrene, the bacterial cell density was monitored by measuring the O.D600. Extracellular protein and bacterial count were determined. The degradation percentage of pyrene was quantified by high-performance liquid chromatography (HPLC).The isolate MAM-P39, which has been identified by 16S rDNA, was the best pyrene degrader. This isolate was identified as Pseudomonas panipatensis MAM-P39 with accession number MF150314. It could degrade 90.5% and 66.03% of 500 and 2000 μM pyrene. Degradation products of pyrene were identified by gas chromatography-mass spectrometry (GC-MS) analysis.
以芘为唯一碳源和能量源,采用选择性富集的方法从石油污染土壤中分离出一株天然分离细菌MAM-P39。在芘上生长过程中,通过测定od600来监测细菌的细胞密度。测定细胞外蛋白和细菌计数。采用高效液相色谱法测定芘的降解率。经16S rDNA鉴定,分离得到的MAM-P39是最好的芘降解菌。该分离物经鉴定为帕尼帕特假单胞菌MAM-P39,鉴定号为MF150314。对500 μM和2000 μM芘的降解率分别为90.5%和66.03%。采用气相色谱-质谱联用技术对芘的降解产物进行了鉴定。
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引用次数: 5
Distribution of Actionmycetes in Suez Gulf (Egypt) and Optimization of the Nanoparticles Production of Some Isolates 埃及苏伊士湾行动菌的分布及部分分离菌纳米粒生产的优化
Pub Date : 2018-05-01 DOI: 10.18576/JEHE/060203
G. Abou-elela, H. Abd-Elnaby, U. M. Abdel-Raouf, Moaz M. Hamed
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引用次数: 0
Monitoring of the Hydrochemistry of the Iskanderkul Lake of the Republic of Tajikistan and the Flowing Rivers 塔吉克斯坦共和国伊斯坎德库尔湖和流动河流的水化学监测
Pub Date : 2018-05-01 DOI: 10.18576/JEHE/060201
P. Normatov, Robiya I. Normatova
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引用次数: 0
Preliminary Survey of Wetland in Ethiopia, Threats, Extent of Degradation, and Future Perspective:-A Review Paper 埃塞俄比亚湿地的初步调查、威胁、退化程度及未来展望:综述
Pub Date : 2018-05-01 DOI: 10.18576/JEHE/060204
Dagne Tafa
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引用次数: 3
Influence of Different Moisture Contents and Temperature on Growth and Production of Aflatoxin B1 by a Toxigenic Aspergillus flavus Isolate in Wheat Flour 小麦面粉中不同水分和温度对产毒黄曲霉分离物生长和产生黄曲霉毒素B1的影响
Pub Date : 2017-09-01 DOI: 10.18576/JEHE/050302
A. Hassane, A. El-Shanawany, N. Abo-Dahab, A. Abdel-Hadi, U. Abdul-Raouf, M. Mwanza
Experimental short time population and production of aflatoxin B1 by Aspergillus flavus in wheat flour at 20, 25, 30, 35 and 40oC, as related to different moisture contents 5%, 8%, 10%, 12%, 14%, 16%, 18%, 20%, 22% and 25%, was studied. Spore suspension of a toxigenic A. flavus containing ~ 3×10 spore/ml was inoculated in each flask containing 50 g wheat flour and incubated for 15 days. Spore count was observed slightly in flour with a moisture content 5% at all incubation temperature, then increased gradually by rising the moisture content reaching the maximum at 30oC with moisture content 25%. High performance liquid chromatography analysis of AFB1 showed the lowest quantification (482 ng/kg) at 20oC with 8% moisture content in comparison to uninoculated control. The higher quantity of AFB1 (637835.4 ng/kg) was produced at 25oC with 25% moisture content. Accumulation of high amounts of AFB1 was noticed at 20, 30 and 35oC with moisture content 25%. However, at 40oC with moisture contents 22% and 25%, high spore count was recorded, but traces of AFB1 were produced. The significance of this investigation is that, in the event of wheat flour contamination with afltoxigenic species, rise in moisture contents are hazardous in the storage of wheat flour around 25oC.
研究了在20、25、30、35和40℃条件下,黄曲霉在小麦粉中不同含水率(5%、8%、10%、12%、14%、16%、18%、20%、22%和25%)短时种群数量和黄曲霉毒素B1产量的关系。将含有~ 3×10孢子/ml的产毒黄曲霉孢子悬浮液接种于每瓶50 g小麦粉中,孵育15 d。在所有孵育温度下,含水量为5%的面粉孢子数均略有增加,随后随着含水量的增加孢子数逐渐增加,在30℃时达到最大值,含水量为25%。高效液相色谱分析表明,与未接种对照相比,AFB1在20℃、8%水分条件下的定量最低,为482 ng/kg。在25℃、25%水分条件下,AFB1的产量最高(637835.4 ng/kg)。在20、30和35℃,水分含量为25%的条件下,AFB1大量积累。然而,在40℃,水分含量22%和25%时,孢子数较高,但产生了微量AFB1。本研究的意义在于,在小麦面粉被黄曲霉毒素污染的情况下,小麦面粉在25℃左右储存时,水分含量升高是危险的。
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引用次数: 25
Phytochemical and Antimicrobial of Latex Serum of Calotropis Procera and its Silver Nanoparticles Against Some Reference Pathogenic Strains 原牛角虫乳胶血清及其银纳米颗粒对部分参考病原菌的植物化学及抗菌作用
Pub Date : 2017-09-01 DOI: 10.18576/JEHE/050301
Mohammad. H. A. Hassan, M. Ismail, A. Moharram, A. Shoreit
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引用次数: 12
Ovarian Extract Promotes Proliferation and Upregulates Pluripotency of Mesenchymal Stromal Cells 卵巢提取物促进间充质间质细胞增殖和上调多能性
Pub Date : 2016-09-01 DOI: 10.18576/JEHE/040303
A. El-Badawy, M. Amer, B. Wadie, Dalia Fleifel, A. AlOkda, N. El-Badri
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引用次数: 0
Antagonistic Activity of Actinomyces Isolated from Azerbaijan’s Soils 阿塞拜疆土壤放线菌拮抗活性研究
Pub Date : 2016-09-01 DOI: 10.18576/JEHE/040302
Behboodi Elham, Hasanova Sevda
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引用次数: 0
Iron Regulation of Growth and Heterocyst Formation in the Nitrogen Fixing Cyanobacterium Nostoc sp. PCC 7120 铁对固氮蓝藻Nostoc sp. pcc7120生长和杂囊形成的调控
Pub Date : 2016-09-01 DOI: 10.18576/JEHE/040301
Wafaa Aly, S. Andrews
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引用次数: 4
Urban Tree Leaf as Bio-indicator for Air Pollution around Superphosphate Fertilizers Plant, Upper Egypt 城市树叶对上埃及过磷酸钙化肥厂周围空气污染的生物指示
Pub Date : 2016-05-01 DOI: 10.18576/JEHE/040207
A. El-khatib, A. El-Shanawany, E. M. El-Amery
For the purpose of this study, two common tree species namely: Ficus nitida and Eucalyptus golobulus were selected in the areas surrounding Asyut Superphosphate Fertilizer Plant, Upper Egypt. Physiological and anatomical responses of tree leaf due to airborne particles deposition were studied. Leaves exhibited reduction in their surface area, dry weight, chlorophyll contents, and an increase of carotenoids content. Anatomical features exhibited highly significant variations between control and polluted sites. All the measured parameters showed seasonal and plant species effects, where F. nitida appeared to be more sensitive than E. globulus. Correlation between leaf characteristics and the mass of captured leaf deposit reflects the existence of an association in between and the drastic conditions caused by the prevailing atmospheric pollution, recommend their using as biomarkers.
本研究选取了上埃及Asyut过磷酸钙化肥厂周边地区常见的两种树种:无花果和桉树。研究了大气颗粒物沉降对树木叶片的生理和解剖反应。叶片的表面积、干重、叶绿素含量减少,类胡萝卜素含量增加。解剖特征在对照和污染部位之间表现出高度显著的差异。所有测量参数均显示季节和植物种类的影响,其中乳酸菌比球芽孢杆菌更敏感。叶片特征与捕获叶片沉积物质量之间的相关性反映了普遍大气污染引起的剧烈条件之间存在关联,建议将其用作生物标志物。
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引用次数: 5
期刊
Journal of Ecology and Health
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