E. Nabti, N. Mokrane, M. Ghoul, H. Manyani, M. Dary, M. Megias
New bacterial strains of Bacillus licheniformis and sp were isolated from the rhizosphere of potato, in front of the sea (Bejaia, Algeria). Strains were identified by phenotypic analyses: Gram s taining, mobility, catalase, oxidase, form of colony and cell morphology. Metabolic characters such carbon source, sugars, organic acids, aerobic/anaerobic respiratory system are revealed by API system 50CHB/E. The 16SrRNA gene was isolated and sequenced (1500 bp), and the phylogenic tree was established (bootstrap method). The first strain (RBA-E23) showed a very high similarity to Bacillu s licheniformis (DQ082997.1) (98%) and B. licheniformis (AF372616.1), but the second (RBA-E32) is classified as the out-gro up. Strains are halotolerants 300 and 400 mM/l NaCl, respectively. They used glycine betaine and proline as synthetic osmoprotectants, and marine algal extracts of Ulva lactucaas natural osmoprotectant. The two strains produce: high amount of auxine-phytohormone (IAA: 78 and 101 mg/l); different enzymes such as: cellulase, chitinase, protease, lipase, amylase and urease; siderophores and solubilize ino rganic phosphorus. The study of antifungal strains activity toward nine phyto pathogenic fungi Verticillium dahlia, Fusarium oxysporum, Botryotinia fuckiliana, Phytophthor a cinamomi, Phytophthora cactorum, Colletotrichum acutatum, Botrytis cinerea, Aspergillus Niger and Aspergillus flavus, shows an interesting inhibition activity with variable percentage of Growth inhibition (PGI %) against phytopathogens. Both of bacteria displayed more benefic characters, while the enzymes can be involved in the organic matter decomposition; utilization of the phytohormone-IAA as simulator of vegetable growth. Siderophores and phosphate solubilization are interesting in the fe rtilization of soil. Finally, strains could have potential application against phytopathogenic fungi.
{"title":"Isolation and Characterization of Two Halophilic Bacillus (B. licheniformis and Bacillus sp) with Antifungal Activity","authors":"E. Nabti, N. Mokrane, M. Ghoul, H. Manyani, M. Dary, M. Megias","doi":"10.12785/JEHE/010102","DOIUrl":"https://doi.org/10.12785/JEHE/010102","url":null,"abstract":"New bacterial strains of Bacillus licheniformis and sp were isolated from the rhizosphere of potato, in front of the sea (Bejaia, Algeria). Strains were identified by phenotypic analyses: Gram s taining, mobility, catalase, oxidase, form of colony and cell morphology. Metabolic characters such carbon source, sugars, organic acids, aerobic/anaerobic respiratory system are revealed by API system 50CHB/E. The 16SrRNA gene was isolated and sequenced (1500 bp), and the phylogenic tree was established (bootstrap method). The first strain (RBA-E23) showed a very high similarity to Bacillu s licheniformis (DQ082997.1) (98%) and B. licheniformis (AF372616.1), but the second (RBA-E32) is classified as the out-gro up. Strains are halotolerants 300 and 400 mM/l NaCl, respectively. They used glycine betaine and proline as synthetic osmoprotectants, and marine algal extracts of Ulva lactucaas natural osmoprotectant. The two strains produce: high amount of auxine-phytohormone (IAA: 78 and 101 mg/l); different enzymes such as: cellulase, chitinase, protease, lipase, amylase and urease; siderophores and solubilize ino rganic phosphorus. The study of antifungal strains activity toward nine phyto pathogenic fungi Verticillium dahlia, Fusarium oxysporum, Botryotinia fuckiliana, Phytophthor a cinamomi, Phytophthora cactorum, Colletotrichum acutatum, Botrytis cinerea, Aspergillus Niger and Aspergillus flavus, shows an interesting inhibition activity with variable percentage of Growth inhibition (PGI %) against phytopathogens. Both of bacteria displayed more benefic characters, while the enzymes can be involved in the organic matter decomposition; utilization of the phytohormone-IAA as simulator of vegetable growth. Siderophores and phosphate solubilization are interesting in the fe rtilization of soil. Finally, strains could have potential application against phytopathogenic fungi.","PeriodicalId":106668,"journal":{"name":"Journal of Ecology and Health","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2013-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128720739","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Mixtures of thyme and rifampicin were tested for inhibitory activity against 4 5 Staphylococcus aureusisolates alone and in combination. The results indicate that MICs of thyme extract mixed with rifampicin for all tested strains of S. aureus ranged between 0.2g/ml of rifampicin plus 5 mg/ml of Thyme ethyl-acetate extract or Thyme methanol extract, and 16 g/ml of rifampicin plus 6 mg/ml of Thyme ethyl-acetate extract or Thyme methanol extract. Time-killing curve of rifampicin in combination with Thyme ethyl-acetate extract and with thyme methanol extract exhibit zero CFU after 8hrs, 10hrs respectively incubation period at 37 � C that was compared with time-killing curve of rifampicin, time-killing curve of Thyme ethyl-acetate e xtract and Thyme Methanol extract
{"title":"Antibacterial Activities of THYMUS VULGARIS and Rifmpicin on Methicillin Resistant STAPHYLOCOCCUS AUREUS (MRSA)","authors":"Salah G. Ali, E. Bakhiet, U. Abdul-Raouf","doi":"10.12785/JEHE/010103","DOIUrl":"https://doi.org/10.12785/JEHE/010103","url":null,"abstract":"Mixtures of thyme and rifampicin were tested for inhibitory activity against 4 5 Staphylococcus aureusisolates alone and in combination. The results indicate that MICs of thyme extract mixed with rifampicin for all tested strains of S. aureus ranged between 0.2g/ml of rifampicin plus 5 mg/ml of Thyme ethyl-acetate extract or Thyme methanol extract, and 16 g/ml of rifampicin plus 6 mg/ml of Thyme ethyl-acetate extract or Thyme methanol extract. Time-killing curve of rifampicin in combination with Thyme ethyl-acetate extract and with thyme methanol extract exhibit zero CFU after 8hrs, 10hrs respectively incubation period at 37 � C that was compared with time-killing curve of rifampicin, time-killing curve of Thyme ethyl-acetate e xtract and Thyme Methanol extract","PeriodicalId":106668,"journal":{"name":"Journal of Ecology and Health","volume":"45 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2013-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"129765163","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2013-03-01DOI: 10.5567/SCIINTL.2013.39.50
Abdelghafar M. Abu-Elsaoud, S. Tuleukhanov
Recently the biostimulatory role of physical methods have been observed in major agricultural crop plants, He-Ne laser is one of the most significant biophysical method for enhancing not only seed germination but also plant growth and development. Seeds of wheat cultivars from Kazakhstan (cultivars; aksay, kazakhstanskaya-10 and eretrospermum-350) and from Egypt (cultivar; sakha-168) were exposed to He-Ne laser irradiations with 5 mW. mm −2 power intensity for 1 to 1800 seconds exposition time. Various germination and growth parameters and indices were recorded and calculated. Changes on the rate of superoxide dismutase activity, lipid peroxidation level and hydrogen peroxide accumulation were monitored. Wheat seed pre-treatment caused significant increase in various germination parameters including germination percentage, germination rate (seed.h −1 ), relative germination rate coefficient ( Wk) and enhancing various growth parameters. Several doses of He-Ne laser promoted the germination to a maximum of 100 germination, increasing also the germination rate. Moreover, seed pretreatment with different doses of He-Ne laser caused significant changes in levels of hydrogen peroxide, lipid peroxidation an d Cu-Zn superoxide dismutase activity. Hydrogen peroxide accumulation and lipid peroxidation levels increased dramatically with the incre asing doses of helium neon laser compared to control. However Cu-Zn superoxide dismutase activities dropped following seed pretreatment with He-Ne laser compared to control.
{"title":"Can He-Ne Laser Induce Changes in Oxidative Stress and Antioxidant Activities of Wheat Cultivars from Kazakhstan and Egypt?","authors":"Abdelghafar M. Abu-Elsaoud, S. Tuleukhanov","doi":"10.5567/SCIINTL.2013.39.50","DOIUrl":"https://doi.org/10.5567/SCIINTL.2013.39.50","url":null,"abstract":"Recently the biostimulatory role of physical methods have been observed in major agricultural crop plants, He-Ne laser is one of the most significant biophysical method for enhancing not only seed germination but also plant growth and development. Seeds of wheat cultivars from Kazakhstan (cultivars; aksay, kazakhstanskaya-10 and eretrospermum-350) and from Egypt (cultivar; sakha-168) were exposed to He-Ne laser irradiations with 5 mW. mm −2 power intensity for 1 to 1800 seconds exposition time. Various germination and growth parameters and indices were recorded and calculated. Changes on the rate of superoxide dismutase activity, lipid peroxidation level and hydrogen peroxide accumulation were monitored. Wheat seed pre-treatment caused significant increase in various germination parameters including germination percentage, germination rate (seed.h −1 ), relative germination rate coefficient ( Wk) and enhancing various growth parameters. Several doses of He-Ne laser promoted the germination to a maximum of 100 germination, increasing also the germination rate. Moreover, seed pretreatment with different doses of He-Ne laser caused significant changes in levels of hydrogen peroxide, lipid peroxidation an d Cu-Zn superoxide dismutase activity. Hydrogen peroxide accumulation and lipid peroxidation levels increased dramatically with the incre asing doses of helium neon laser compared to control. However Cu-Zn superoxide dismutase activities dropped following seed pretreatment with He-Ne laser compared to control.","PeriodicalId":106668,"journal":{"name":"Journal of Ecology and Health","volume":"85 5 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2013-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130788214","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Saleh. G. Ali, M. S. Mohammad, E. Bakhiet, S. Hassan
{"title":"Comparative Study on Two Different Up Scale Locally Available Construction Microbial Fuel Cells","authors":"Saleh. G. Ali, M. S. Mohammad, E. Bakhiet, S. Hassan","doi":"10.18576/JEHE/060102","DOIUrl":"https://doi.org/10.18576/JEHE/060102","url":null,"abstract":"","PeriodicalId":106668,"journal":{"name":"Journal of Ecology and Health","volume":"49 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122176210","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Survey of the Most Common Insect Species on Some Foraging Crops of Honeybees in Dakhla Oasis, New Valley Governorate, Egypt","authors":"Mahmoud S. O. Mabrouk, M. Mahbob","doi":"10.18576/JEHE/050105","DOIUrl":"https://doi.org/10.18576/JEHE/050105","url":null,"abstract":"","PeriodicalId":106668,"journal":{"name":"Journal of Ecology and Health","volume":"11 8 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131626881","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A. Farrag, R. Shehata, H. El-Sheikh, N. Abo-Dahab, A. Ali
Thirty fungal isolates, which include nineteen species and belong to five genera, were isolated from unusual soil samples from Assiut and Wadi El-Natrun, Egypt. All fungal cultures were cultivated on YES medium for secondary metabolites production; the culture filtrates were separately subjected to solvent extraction by: Ethyl Acetate, Petroleum Ether, Dichloromethane and Dichloromethane: Methanol (2: 1); the fungal mat was extracted by Dichloromethane: Methanol (2: 1) only. The obtained Ethyl Acetate extracts of all fungal isolates were tested for their antimicrobial activity against six different pathogenic bacterial strains; three of them were gram positive bacteria (Staphylococcus Aureus, Bacillus Cereus, and Streptococcus Pneumonia); the others were gram negative bacteria (Sertatia Marcescens, E. Coli and Salmonella Typhi). In addition, against five pathogenic fungal strains (Aspergillus Candidus, Aspergillus Flavus, Aspergillus Niger, Aspergillus Terreus and Geotrichum Candidum), the tested Ethyl Acetate fungal extract showed marked antimicrobial activity against most of the tested strains. On the other hand, the highest antibacterial activity was recorded in case of the Penicillium Chrysogenum extract, but the highest antifungal activity was recorded in case of the Penicillium Aurantiogriseum extract. The secondary metabolites of all fungal isolates were separated on TLC plates, and the most four active strains were completely identified as Aspergillus Candidus, Aspergillus Terreus, Penicillium Aurantiogriseum and Penicillium Chrysogenum. Furthermore, the antimicrobial, anti-insect, anti-herbal and anti-tumor activities of its extracts were evaluated.
{"title":"Diversity and Biotechnological Applications of Some Fungi that Isolated from Unusual Soil Samples in Egypt","authors":"A. Farrag, R. Shehata, H. El-Sheikh, N. Abo-Dahab, A. Ali","doi":"10.18576/JEHE/050104","DOIUrl":"https://doi.org/10.18576/JEHE/050104","url":null,"abstract":"Thirty fungal isolates, which include nineteen species and belong to five genera, were isolated from unusual soil samples from Assiut and Wadi El-Natrun, Egypt. All fungal cultures were cultivated on YES medium for secondary metabolites production; the culture filtrates were separately subjected to solvent extraction by: Ethyl Acetate, Petroleum Ether, Dichloromethane and Dichloromethane: Methanol (2: 1); the fungal mat was extracted by Dichloromethane: Methanol (2: 1) only. The obtained Ethyl Acetate extracts of all fungal isolates were tested for their antimicrobial activity against six different pathogenic bacterial strains; three of them were gram positive bacteria (Staphylococcus Aureus, Bacillus Cereus, and Streptococcus Pneumonia); the others were gram negative bacteria (Sertatia Marcescens, E. Coli and Salmonella Typhi). In addition, against five pathogenic fungal strains (Aspergillus Candidus, Aspergillus Flavus, Aspergillus Niger, Aspergillus Terreus and Geotrichum Candidum), the tested Ethyl Acetate fungal extract showed marked antimicrobial activity against most of the tested strains. On the other hand, the highest antibacterial activity was recorded in case of the Penicillium Chrysogenum extract, but the highest antifungal activity was recorded in case of the Penicillium Aurantiogriseum extract. The secondary metabolites of all fungal isolates were separated on TLC plates, and the most four active strains were completely identified as Aspergillus Candidus, Aspergillus Terreus, Penicillium Aurantiogriseum and Penicillium Chrysogenum. Furthermore, the antimicrobial, anti-insect, anti-herbal and anti-tumor activities of its extracts were evaluated.","PeriodicalId":106668,"journal":{"name":"Journal of Ecology and Health","volume":"5 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130858628","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Research of Improved Case adaptation Technology for Steelmaking and Concasting Dynamic Scheduling","authors":"Guozhang Jiang, Juan Du, Gongfa Li","doi":"10.18576/JEHE/050102","DOIUrl":"https://doi.org/10.18576/JEHE/050102","url":null,"abstract":"","PeriodicalId":106668,"journal":{"name":"Journal of Ecology and Health","volume":"91 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115667979","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Afat O. Mammadova, Narmin M. Farzaliyeva, Roza N. Mammadova
{"title":"Environmental Assessment of the Tree Plant Leaves According to their Physiological State and Fluctuating Asymmetry Indices of Morphological Features, Which Widely Spread in Baku","authors":"Afat O. Mammadova, Narmin M. Farzaliyeva, Roza N. Mammadova","doi":"10.18576/JEHE/050103","DOIUrl":"https://doi.org/10.18576/JEHE/050103","url":null,"abstract":"","PeriodicalId":106668,"journal":{"name":"Journal of Ecology and Health","volume":"17 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130945774","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
H. El-Sharkawi, K. Farghali, A. Rayan, Dalia M. Sayed
{"title":"Changes in Contents of Free Amino Acids in Germinating Seed Organs of Three Senna Species under Temperature, Osmotic Stress and Zinc Concentration","authors":"H. El-Sharkawi, K. Farghali, A. Rayan, Dalia M. Sayed","doi":"10.18576/jehe/060105","DOIUrl":"https://doi.org/10.18576/jehe/060105","url":null,"abstract":"","PeriodicalId":106668,"journal":{"name":"Journal of Ecology and Health","volume":"132 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132154986","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A. Obuid-Allah, A. Mahmoud, Elamier. H. M. Hussien
{"title":"Population Dynamics of Terrestrial Spiders (Arachnida) at Qena Governorate, Upper Egypt","authors":"A. Obuid-Allah, A. Mahmoud, Elamier. H. M. Hussien","doi":"10.18576/JEHE/060106","DOIUrl":"https://doi.org/10.18576/JEHE/060106","url":null,"abstract":"","PeriodicalId":106668,"journal":{"name":"Journal of Ecology and Health","volume":"369 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"116324089","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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