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Effect of UVA+B on Germination Consequences, Oxidative Stress and Antioxidant Defence Mechanisms of Wheat Triticum aestivum L. UVA+B对小麦萌发、氧化胁迫及抗氧化防御机制的影响
Pub Date : 2016-05-01 DOI: 10.18576/JEHE/040204
Abdelghafar M. Abu-Elsaoud, H. Hassan
Stratospheric ozone depletion will increase the solar ultraviolet radiations especially in the range UV-A and UVB. Increased levels of UV radiations affect and cause damage on cellular level on various organisms. Current research study was targeted to evaluate the effect of seed pretreatment with ultraviolet radiation on germination, oxidative damage and some antioxidant enzymes of three common wheat (Triticum aestivum L.) cultivars namely; Sakha-94, Gemmiza-9 and Giza-168. Seeds were irradiated to ultraviolet radiation with wavelength from 300-410 nm and maximum wavelengths of 350 nm for 6 different exposure doses (1, 5, 10, 30, 60 and 120 s). Various germination indices, oxidative stress and protective enzymes were estimated and recorded every two weeks till harvest of experiment. Ultraviolet radiation induced increases in germination consequences of wheat cultivars especially seed germination percentage and germination rate. Seed pretreatment with ultraviolet radiations induced a significant decrease on various oxidative stress damage consequences both hydrogen peroxide level and lipid peroxidation, which were monitored biweekly till harvest. Antioxidant enzymes estimated; superoxide dismutase, catalase and ascorbic acid peroxidase showed a huge and significant activation after seed pretreatment with different doses of UV-light. Data provide a new trend in priming using ultraviolet radiations of wavelengths range UV-A and UV-B
平流层臭氧耗竭将增加太阳紫外线辐射,特别是UV-A和UVB范围内的紫外线辐射。紫外线辐射水平的增加对各种生物体的细胞水平产生影响并造成损害。本研究旨在研究紫外辐照对小麦(Triticum aestivum L.)种子萌发、氧化损伤及部分抗氧化酶的影响。Sakha-94, Gemmiza-9和Giza-168。将种子照射在波长300 ~ 410 nm、最大波长350 nm的紫外线下,照射时间分别为1、5、10、30、60和120 s,每隔2周测定并记录种子萌发指数、氧化应激和保护酶的变化,直至收获。紫外辐射对小麦品种的萌发效果有显著影响,尤其是种子发芽率和发芽率。对种子进行紫外线预处理后,两周监测过氧化氢水平和脂质过氧化水平,显著降低了种子的各种氧化应激损伤。抗氧化酶估计;不同剂量的紫外线对种子进行预处理后,超氧化物歧化酶、过氧化氢酶和抗坏血酸过氧化物酶均表现出显著的活化作用。数据提供了利用波长范围为UV-A和UV-B的紫外线辐射引发的新趋势
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引用次数: 1
Pathway of 1,2,4-Trichlorobenzene Degradation by Bacillus mucilaginosus Isolated from Petroleum Polluted Soils 石油污染土壤中黏液芽孢杆菌降解1,2,4-三氯苯的途径
Pub Date : 2016-05-01 DOI: 10.18576/jehe/040201
M. Abo-State, Y. Saleh, O. Khalil
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引用次数: 0
Efficacy of Antibiotic Combinations on Multi-Drug Resistant Bacterial Strains Isolated from Urinary Tract Infection and Hemodialysis Patients 联合抗生素对尿路感染和血液透析患者多重耐药菌株的疗效观察
Pub Date : 2016-05-01 DOI: 10.18576/JEHE/040202
M. Abo-State, Y. Saleh, S. Fathy
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引用次数: 1
Salvia Lanigera var. grandiflora Benth: A New Record in the Flora of Egypt 标题大花鼠尾草:埃及植物区系新记录
Pub Date : 2016-05-01 DOI: 10.18576/jehe/040205
A. El-Banhawy, W. Kamel, E. G. Eldin
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引用次数: 3
Analytical Solution of One Dimension Time Dependent Advection- Diffusion Equation 一维随时间变化的平流扩散方程的解析解
Pub Date : 2016-05-01 DOI: 10.18576/JEHE/040203
A. Marrouf, M. El-Otaify, A. S. Mohamed, Galal Ismail, K. Essa
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引用次数: 0
Legumes Dominated Steppe Vegetation Type in the Flora of Azerbaijan 阿塞拜疆草原植物区系中豆科植物为主的植被类型
Pub Date : 2016-05-01 DOI: 10.18576/JEHE/040206
Z. Mammadova, E. Gurbanov, A. Akhundova
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引用次数: 0
Impact of Diazotrophic Bacteria on Germination and Growth of Tomato, with Bio-control Effect, Isolated from Algerian Soil 阿尔及利亚土壤重氮营养菌对番茄萌发和生长的生物防治作用
Pub Date : 2014-05-01 DOI: 10.12785/JEHE/020101
N. Tabli, E. Nabti, D. Dahel, N. Mokrane, H. Manyani, M. Dary, M. Megias
soils samples were collected from the west region of Bejaia-Algeria in aseptic conditions. The preliminary germination test on tomatos seeds allowed screening of 4 simples from which 40 different colonies were isolated and purified. These last were submitted to in vitro germination test, while 20 strains with an inductor effect on th e germination were selected and tested for the presence of stimulator effect of growth of tomato in vivo. Biochemical (on API 50 CHB/E gallery), and phylogenetic (DNAr 16S sequencing) identification, tests researching characteristics promoting p lant growth (production of IAA (Indol Acetic Acid) phyto- hormone, enzymes, siderophores, free nitrogen fixation, and an an tagonistic test on five phytopathogenic fungi ( Botrytis cinerea; Phytophtora cactorum, phytophtora cinnamomi; Fusarium oxysporum a nd Verticillium dahliae) were applied for the strain showing positive in vivo germination results on tomato.The results of this last test led to select four strains: S11, S6, S15, and S12 that greatly affected tomato growth, with stems length (cm) of 9.212, 9.280, 9.362 and 9.858, respectively, compared to the control (6.400). Biochemical and phylogenic identifications of these strains allowed to affiliate them as following: Arthrobacter agilis(S6), Streptomyces Sp (S11), Bacillus sp13 (S15) and Mycobacterium Sp(S12). The four strains produced several enzymes, high indol acetic acid (phyto- hormone) quantity (mg/l: 38.47 (S11); 86.90 (S6); 93.96 (S12) and 190.23 (S15), siderophores, nitrogen fixing. It also showed an antagonistic activity against the phytopathogenic fungi tested with variable p lant growth inhibition (PGI) %:0-78.51% for Bacillus sp13; 29-74%for Streptomyces sp; 0-72%for Arthrobacter agilis; and 0-69% for Mycobacterium sp. These results prove that the strains are compatible for tomato growth stimulation.
土壤样品在无菌条件下采集自阿尔及利亚西部地区。对番茄种子进行初步萌发试验,筛选出4个样品,分离纯化了40个菌落。选取20株对番茄萌发有诱导作用的菌株,在体内进行了对番茄生长有刺激作用的试验。生物化学(API 50 CHB/E gallery)和系统发育(DNAr 16S测序)鉴定,研究促进植物生长的特性(IAA(吲哚乙酸)植物激素、酶、铁载体、游离固氮的产生,以及对五种植物病原真菌(Botrytis cinerea;仙人掌植物,肉桂植物;用尖孢镰刀菌和大丽花黄萎病菌(Verticillium dahliae)在番茄体内萌发阳性。结果表明,菌株S11、S6、S15和S12对番茄生长影响较大,茎长(cm)分别为9.212、9.280、9.362和9.858,高于对照(6400 cm)。这些菌株的生化和系统发育鉴定表明它们属于以下菌株:灵巧节杆菌(S6),链霉菌Sp(S11),芽孢杆菌sp13 (S15)和分枝杆菌Sp(S12)。4株菌株产生多种酶,吲哚乙酸(植物激素)含量高(mg/l: 38.47 (S11);86.90 (S6);93.96 (S12)和190.23 (S15),铁载体,固氮。对多种植物病原真菌均有拮抗作用,对芽孢杆菌sp13的植物生长抑制(PGI) %:0 ~ 78.51%;链霉菌29-74%;灵巧节杆菌0 ~ 72%;分枝杆菌对番茄生长的抑制率为0 ~ 69%,证明两菌株对番茄生长刺激具有亲和性。
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引用次数: 2
Cortical Dynamics as a Therapeutic Mechanism for Touch Healing 皮层动力学作为触觉愈合的治疗机制
Pub Date : 2014-05-01 DOI: 10.12785/JEHE/020103
Morteza Alibakhshi Kenari
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引用次数: 3
Renewable Energies Environmental Impacts: Wind Energy Case 可再生能源环境影响:风能案例
Pub Date : 2014-05-01 DOI: 10.12785/JEHE/020102
Haddad Louiza, A. Zéroual
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引用次数: 0
A finite Element Model for the Electrical Activity in Human Cardiac Tissues 人体心脏组织电活动的有限元模型
Pub Date : 2013-05-01 DOI: 10.12785/JEHE/010104
S. M. Shuaiby, Mohsen A. Hassan, A. Sharkawy, A. M. Gad
Biosimulation models of the heart action potential have become a very useful tool. It provides better understanding for the complex biophysical phenomena related to electrical activity in the heart s uch as cardiac arrhythmias. At cellular level, the electrical activity of cardiac tissues may be simulated by solving a system of ordinary deferential equations (ODEs) describing the electrical behavior of the cell membrane. Because the biophysical processes underlying this phenomenon are non-linear and change very rapidly, the ODE system is a challenge to be solved numerically. Furthermore, the implementation of these models is a hard task for commercial finite element software. In this paper a finite element formulation, model a nd code generation of monodomain equation has been conducted. The developed code is coupled with the modified FitzHugh-Nag umo (FHN) cell electrophysiological model in order to have isotropic excitation propagation starting from cell level to complete hea rt level. MTALAB programming language was used to build the proposed standalone finite element code. A two dimensional spec imen of heart tissues is simulated to show the behavior of the excitation propagation and the repolarization phase for isotropic electric al activity. Simulation results of the cardiac action potential have shown good agreements with the experimental measurements obtained from published literature.
心脏动作电位的生物模拟模型已经成为一个非常有用的工具。它可以更好地理解与心脏电活动相关的复杂生物物理现象,如心律失常。在细胞水平上,心脏组织的电活动可以通过求解描述细胞膜电行为的普通微分方程(ode)系统来模拟。由于这种现象背后的生物物理过程是非线性的,变化非常迅速,因此ODE系统是一个需要用数值方法解决的挑战。此外,这些模型的实现对于商业有限元软件来说是一项艰巨的任务。本文对单域方程进行了有限元计算、模型建立和代码生成。所开发的代码与改进的FitzHugh-Nag umo (FHN)细胞电生理模型相结合,以实现从细胞水平到整个心脏水平的各向同性激励传播。采用MTALAB编程语言编制了所提出的独立有限元代码。模拟了心脏组织的二维谱图,研究了各向同性电活动的激励传播和复极化相位。心脏动作电位的模拟结果与已发表文献的实验结果吻合较好。
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引用次数: 5
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Journal of Ecology and Health
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