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Genetic Conservation from the Perspective of Sustainable Development to Guarantee Safety in Bovine Dairy Production 可持续发展视角下的遗传保护保障奶牛生产安全
Pub Date : 2023-01-19 DOI: 10.54026/cjdvs1050
A. C. Núñez
For this study, 441 racial groups were used, using the following crosses: 7/8 Holstein X 1/8 Guzerat (n= 112), 7/8 American Swiss x 1/8 Guzerat (n=138) and 7/8 dairy Simmental X 1/8 Guzerat (n=191), from three dualpurpose farms in northern Puebla; Mexico. With the objective of evaluating milk production per lactation (PTL), period of initiation of lactation (EIL), days to peak lactation (PL), behavior of calves until weaning (CBD) and economic productivity. The results obtained indicated that milk production per lactation and days in lactation were affected by the genotype (P<.01). The productive variables for the behavior until weaning of calves of 7/8 European dairy cows x 1/8 Guzerat in dual purpose included; birth weight (BN), weaning weight (PD), daily weight gain (GDP) and weaning age (ED) were evaluated by survival analysis. The results indicated that the racial group did not influence (P>.01) any of the productive characteristics studied. In conclusion, 7/8 Holstein x 1/8 Guzerat (HG) and 7/8 American Swiss x 1/8 Guzerat (SAG) cows showed higher productive ability than cows with Simmental genes. The observed results suggest the possibility of using these crosses, in order to maintain the perspective of sustainable development, guaranteeing safety in bovine milk production in the northern region of Puebla.
在这项研究中,使用了441个种族群体,使用以下杂交:7/8荷尔斯坦X 1/8古泽拉(n= 112), 7/8美国瑞士X 1/8古泽拉(n=138)和7/8乳制品西蒙塔尔X 1/8古泽拉(n=191),来自普埃布拉北部的三个双重用途农场;墨西哥。目的是评价每次泌乳量(PTL)、泌乳起始期(EIL)、至泌乳高峰天数(PL)、犊牛断奶前行为(CBD)和经济生产力。结果表明,单泌乳量和泌乳天数均受基因型的影响(p < 0.01)。综上所述,7/8荷斯坦× 1/8古泽拉(HG)和7/8美国瑞士× 1/8古泽拉(SAG)奶牛的生产能力高于西门塔尔基因奶牛。观察结果表明,为了保持可持续发展的前景,使用这些杂交的可能性,保证了普埃布拉北部地区的牛奶生产安全。
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引用次数: 0
Fecal Microbiota of Rhinoceros Unicornis as a Reservoir of Multi-Drug-Resistant Bacteria 独角犀牛粪便微生物群作为多重耐药细菌的宿主
Pub Date : 2023-01-04 DOI: 10.54026/cjdvs1049
Sangeeta Das
The presence of Antimicrobial Resistance (AMR) bacteria in wildlife indicates the possible role of wild animals as efficient AMR reservoirs and dispersers of resistant bacteria to the human, livestock and natural environments. The presence of AMR bacteria not only has serious public health consequences, but also threatens native wildlife populations. In this study, we investigate the occurrence and antibiotic resistance patterns of fecal microbiota of Rhinoceros unicornis in Assam. Sixty two freshly voided dung samples of rhinoceros were collected from Kaziranga National Park of Assam in 2018. Fecal samples were tested for the presence of bacterial species and submitted to National Centre for Veterinary Type Cultures, National Research Centre on Equines, (NC-VTCC, NRCE) Hisar for identification. Antimicrobial Susceptibility Testing (AST) was determined using the disk diffusion method and antibiotic resistance patterns were assessed according to the Clinical and Laboratory Standards Institute (CLSI) recommendations. Overall, 24 isolates were identified that belonged to 19 different bacterial genera including Klebsiella spp., Achromobacter spp., Pseudomonas spp., Alishewanella spp., Wautersiella spp., Moraxella spp., Inquilinus spp., Weeksella spp., Oligella spp., Myroides spp., Paracoccus spp., Ochrobactrum spp., Psychrobacter spp., Pannonibacter spp., Shewanella spp., Sphingobacterium spp., Sphingomonas spp. (4% each), Escherichia coli (8%) and Acinetobacter spp. (21%). Of the 24 isolates found in rhinoceros fecal samples tested for antimicrobial susceptibility, 13 isolates showed resistant to three or more than three substance classes. Results indicate that overall, most of the bacterial species from R. unicornis were multi-drug-resistant, which may reflect not only several risk factors leading to the origin of AMR in wild animals but also wildlife as natural reservoir of resistant bacteria. Therefore, efforts must be initiated to monitor the occurrence of such AMR bacteria in wildlife and understand their potential effect on wildlife conservation and public health.
野生动物中抗菌素耐药性(AMR)细菌的存在表明野生动物可能是人类、牲畜和自然环境中耐药细菌的有效储存库和传播者。抗菌素耐药性细菌的存在不仅对公共卫生造成严重后果,而且还威胁到本地野生动物种群。在这项研究中,我们调查了阿萨姆邦独角犀牛粪便微生物群的发生和抗生素耐药性模式。2018年,阿萨姆邦卡兹兰加国家公园收集了62份新鲜的犀牛粪便样本。对粪便样本进行细菌种类检测,并提交给国家马研究中心(NC-VTCC, NRCE) Hisar国家兽医类型培养中心进行鉴定。采用纸片扩散法进行药敏试验(AST),并根据临床与实验室标准协会(CLSI)的建议评估抗生素耐药模式。共鉴定出24株分离菌,分属19个菌属,包括克雷伯氏菌、无色杆菌、假单胞菌、阿利什瓦氏菌、沃特氏菌、莫拉氏菌、Inquilinus、Weeksella、Oligella、Myroides、副球菌、Ochrobactrum、Psychrobacter、Pannonibacter、Shewanella、Sphingobacterium、Sphingomonas(各占4%)、大肠杆菌(8%)和不动杆菌(21%)。在对犀牛粪便样本进行抗菌素敏感性测试的24个分离株中,13个分离株对三种或三种以上的物质具有耐药性。结果表明,总体而言,大多数独角兽单胞菌属细菌具有多重耐药,这可能反映了野生动物AMR起源的多种危险因素,也可能反映了野生动物是耐药细菌的天然宿主。因此,必须开始努力监测野生动物中此类抗菌素耐药性细菌的发生情况,并了解其对野生动物保护和公众健康的潜在影响。
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引用次数: 0
Reproductive Management in Goat Breeding 山羊育种中的生殖管理
Pub Date : 2022-12-22 DOI: 10.54026/cjdvs1048
J. Grizelj
Goats belong to the „short day breeders“ group of seasonal polyoestrous animals. In the temperate climate zone, their season lasts from mid-summer till mid-autumn, being regulated by epiphysis’ melatonine secretion, as well as by genetics and environment factors. Therefore, the presence of goat milk and dairy products, as well as goat meat, at the food market, is exclusively seasonal. In order to provide goat food products throughout the whole year, reproductive herd management should be established. This paper presents a brief overview of the methods of reproductive management in dairy goat herds (natural mating and artificial insemination, hormonal and nonhormonal synchronization methods and embryo production and transfer). Proposed protocols are consisted of light treatment, male effect and timed artificial insemination which could be combined with or without injection of prostaglandin. Application of reproductive and breeding programs allow efficient diffusion of desirable genetic traits in a selection program.
山羊属于“短日繁殖者”一类的季节性多情动物。在温带气候区,它们的季节从仲夏一直持续到中秋,受骨骺褪黑激素分泌以及遗传和环境因素的调节。因此,在食品市场上出现的山羊奶和奶制品以及山羊肉完全是季节性的。为了全年提供山羊食品,应建立繁殖群管理。本文简要介绍了奶山羊群的生殖管理方法(自然交配和人工授精、激素和非激素同步方法以及胚胎生产和移植)。建议的治疗方案包括光治疗、男性效应和定时人工授精,可联合或不联合注射前列腺素。生殖和育种程序的应用允许在选择程序中有效地传播理想的遗传性状。
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引用次数: 0
Development and Maturation of Microbiota in Cow Rumen, Plant-Fibers Degradation and Influences on the Immune System and Cow Health 奶牛瘤胃微生物群发育成熟、植物纤维降解及其对免疫系统和奶牛健康的影响
Pub Date : 2022-12-05 DOI: 10.54026/cjdvs1047
S. Focardi
Rumen is part of the forestomach of ruminants and plays a key role in the conversion of feed into metabolites that are absorbed and used by the host. The rumen is also the place of formation of proteins of microbial origin, which represent a source of energy for the host animal. From a functional point of view, ruminants are monogastric at birth as they have an undeveloped forestomach system. Microbial communities in the rumen first show colonization by bacteria, followed by that of methanogenic Archaea and then anaerobic fungi and protozoa. In newborn calves, molecular-based techniques evidenced initial rumen colonisation by facultative anaerobic bacteria, as the phyla Proteobacteria and Firmicutes, with genera Enterococcus and Streptococcus and the species Escherichia coli, followed by Archaea within a few hours after birth. These early colonizers utilize the oxygen available in the rumen, thus creating an anaerobic environment conducive to the growth of rigorous anaerobic communities, including Bifidobacterium and Bacteroides. The strict anaerobic bacterial community, including cellulolytic and proteolytic bacteria, establishes and dominates the rumen microbiome within the first two weeks of life. The entire microbial community allows ruminants to use ligno-cellulosic materials and non-protein nitrogen to produce high-quality food. Importantly, these close anaerobic bacterial communities in the rumen of newborns play an essential role in the development of the mucosal immune system. A healthy rumen leads to healthy ruminants with optimal performance. It is worth highlighting the importance of the microbiome in maintaining the health of cattle and its potential in alleviating disease. This mini-review described the development of the cow microbiome in the rumen, the degradation abilities and influence of the feed on the rumen microbiota, and the microbiota effects on the cow’s immune system and health.
瘤胃是反刍动物前胃的一部分,在饲料转化为代谢产物并被宿主吸收和利用的过程中起着关键作用。瘤胃也是微生物来源的蛋白质形成的地方,这代表了宿主动物的能量来源。从功能的角度来看,反刍动物在出生时是单胃的,因为它们的前胃系统不发达。瘤胃微生物群落首先表现为细菌的定植,其次是产甲烷的古细菌,然后是厌氧真菌和原生动物。在新生小牛中,基于分子的技术证实了兼性厌氧细菌在出生后几小时内在瘤胃内的初始定植,如变形杆菌门和厚壁菌门,肠球菌属和链球菌属以及大肠杆菌属,其次是古细菌。这些早期的殖民者利用瘤胃中可用的氧气,从而创造一个有利于严格厌氧群落生长的厌氧环境,包括双歧杆菌和拟杆菌。严格的厌氧细菌群落,包括纤维素分解菌和蛋白分解菌,在生命的前两周内建立并主导着瘤胃微生物群。整个微生物群落允许反刍动物使用木质纤维素材料和非蛋白氮来生产高质量的食物。重要的是,新生儿瘤胃中这些紧密的厌氧细菌群落在粘膜免疫系统的发育中起着至关重要的作用。健康的瘤胃造就了具有最佳生产性能的健康反刍动物。值得强调的是,微生物组在维持牛的健康方面的重要性及其在减轻疾病方面的潜力。本文综述了奶牛瘤胃微生物群的发育、饲料对瘤胃微生物群的降解能力和影响,以及微生物群对奶牛免疫系统和健康的影响。
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引用次数: 0
Evaluation of the Compatibility between Different Teat Sealants and Intramammary Antimicrobials in Selective Dry Cow Treatment Protocols 选择性干牛治疗方案中不同乳封剂与乳内抗菌剂的相容性评价
Pub Date : 2022-11-17 DOI: 10.54026/cjdvs1046
Gazza Carlo,
The dry period is a crucial time in the lactation cycle, it is the period of highest susceptibility to new infection and the optimum time to cure existing intramammary infection. In selective dry-cow antimicrobial therapy, we have two different ways of intervening in the management treatments of dry period: in the first case there are dairy cows diagnosed healthy, that will be treated only with the teat sealant, in the second, dairy cows with pathogenic mastitis or high cell counts that will receive the intramammary treatment together with the sealant. One of the main prerogative of an intramammary sealant is to ensure that it remains in the teat throughout the dry period. To guarantee this, it is necessary that the sealant is characterized by good rheological properties, especially when it is administered with an antimastitis ointment. The aim of the present study was to compare the rheological behavior of several intramammary sealant suspensions present on the Italian market. To evaluate the rheological behavior of the intramammary suspensions used during the dry period, rheological comparison methods have been developed. The parameters assessed during the rheological study are Viscosity, 3 Interval Thixotropy Test (3ITT) and the viscoelastic nature of materials: Amplitude Sweep. In addition, to evaluate the sealant performance of intramammary sealants, was performed a compatibility in-vitro test, comparing the behavior of the products alone and in contact with two intramammary antibiotic suspensions (Fatroximin and Mastout, both manufactured by Fatro S.p.A. and used during the dry period). The results obtained highlighted that, when a sealant has good viscosity and good thixotropic behavior, is more probable that it remains in the teat, carrying out its sealing action and preventing the entry of pathogens.
干乳期是哺乳周期的关键时期,是新发感染的易感性最高的时期,也是治疗已有乳内感染的最佳时机。在选择性干奶牛抗菌治疗中,我们有两种不同的方式介入干期的管理治疗:第一种情况下,奶牛被诊断为健康,只使用乳封剂治疗;第二种情况下,患有致病性乳腺炎或细胞计数高的奶牛将接受乳内治疗和乳封剂。乳内密封剂的主要优点之一是确保它在整个干燥期保持在乳头内。为了保证这一点,有必要使密封剂具有良好的流变特性,特别是当它与抗乳腺炎软膏一起使用时。本研究的目的是比较意大利市场上几种乳内密封胶悬浮液的流变行为。为了评价干期乳内悬浮液的流变行为,流变比较方法已经被开发出来。在流变学研究中评估的参数是粘度,3区间触变性测试(3ITT)和材料的粘弹性性质:振幅扫描。此外,为了评估乳内密封剂的密封性能,进行了相容性体外试验,比较了产品单独使用和与两种乳内抗生素悬浮液(Fatroximin和Mastout,均由Fatro S.p.A.生产,并在干燥期使用)接触时的行为。结果表明,当密封胶具有良好的粘度和良好的触变性时,它更有可能留在乳头中,执行其密封作用并阻止病原体的进入。
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引用次数: 0
A Clinical Case of Chronic Recurrent Multifocal Osteomyelitis in a Dog 犬慢性复发性多灶性骨髓炎1例
Pub Date : 2022-10-19 DOI: 10.54026/cjdvs1045
Daniel J Duffy
A 5-year-old 5.5-kg castrated male Italian Greyhound was evaluated for bone pain, generalized hyperesthesia and fever. Physical and orthopedic examination revealed non-specific bone pain and hyperesthesia; radiographs were then performed. Radiological findings included smooth to irregular periosteal reaction on the caudal, lateral and medial aspect of the proximal right humeral diaphysis. Moth-eaten to permeative lysis was present on the humeral head and metaphysis with mild associated sclerosis. Because the dog’s clinical signs worsened despite instituted medical management, synovial fluid samples were obtained and submitted for cytological analysis and culture. Surgical biopsy specimens were obtained from the right proximolateral diaphysis of the right humerus. Results of histochemical analysis, cytology and culture of bony tissue and blood were negative for bacterial or fungal agents. These clinical, imaging, and histopathologic findings were compatible with Chronic Recurrent Multifocal Osteomyelitis (CRMO). To the author’s knowledge, this is the first report of chronic recurrent multifocal osteomyelitits in a dog. CRMO should be considered as a differential diagnosis in dogs with signs of bone pain with or without fever. Further investigation into therapeutic treatments and causative etiologies in veterinary patients is warranted.
一只5岁的5.5公斤阉割的意大利灰狗被评估为骨痛,全身感觉亢进和发烧。体格和骨科检查显示非特异性骨痛和感觉亢进;然后进行x光片检查。放射学表现包括在右侧肱骨近端尾部、外侧和内侧有平滑到不规则的骨膜反应。肱骨头和干骺端出现虫蛀至渗透性溶解,伴轻度相关硬化。尽管进行了医疗管理,但狗的临床症状恶化,因此获得滑液样本并提交细胞学分析和培养。手术活检标本取自右肱骨近外侧干。组织化学分析、细胞学分析、骨组织和血液培养均未发现细菌或真菌。这些临床、影像学和组织病理学结果与慢性复发性多灶性骨髓炎(CRMO)相符。据作者所知,这是第一次报告慢性复发性多灶性骨髓炎的狗。CRMO应被视为鉴别诊断的狗骨疼痛的迹象,有或没有发烧。进一步调查兽医患者的治疗方法和病因是必要的。
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引用次数: 1
Biomechanical Evaluation of a Novel Double-Strand (Looped) Polyamide Monofilament Suture for Canine Flexor Tendon Repair 新型双股(环)聚酰胺单丝缝线用于犬屈肌腱修复的生物力学评价
Pub Date : 2022-08-29 DOI: 10.54026/cjdvs1044
Daniel J Duffy
Objective: Determine the influence of a novel looped polyamide suture on the biomechanical properties and gap formation of repaired canine flexor tendons. Study Design: Tendons were assigned to 3 groups (n=12/group). Following transection, tendons were repaired with a Kessler pattern using monofilament polypropylene, Kessler pattern using looped polyamide suture and a Kessler pattern using looped polyamide augmented with a continuous Epitendinous Suture (ES) representing groups 1, 2, and 3 respectively. Constructs were tested to simulate clinical failure. Yield, peak and failure loads, loads at -1 and 3mm gap formation and failure modes were analyzed. Results: Looped polyamide suture is equivalent to monofilament polypropylene using a Kessler core pattern. Looped polyamide suture augmented with a running ES significantly increased yield, peak and failure loads by 3.2x, 3.0x and 2.6x respectively, compared to core suture use alone. Use of an ES required significantly greater force to cause 3mm gap formation while reducing occurrence of gapping in tested constructs. Mode of failure differed among experimental constructs. Conclusion: Looped polyamide suture is equivalent to monofilament polypropylene in the same pattern. Our results support the addition of ES augmentation, a simple technique modification that demonstrates substantially improved repair strength while reducing the occurrence of gapping between tendon ends. Future in-vivo studies investigating effect of suture placement on tendinous healing, blood supply, and glide function are warranted.
目的:探讨新型环状聚酰胺缝线对犬屈肌腱修复后生物力学性能及间隙形成的影响。研究设计:将肌腱分为3组(n=12/组)。横断后,分别用单丝聚丙烯、环状聚酰胺缝线和环状聚酰胺增强连续外延缝合(ES)的Kessler模式修复肌腱,分别代表1、2和3组。对构式进行测试以模拟临床失败。分析了屈服载荷、峰值载荷和失效载荷、-1和3mm间隙形成载荷以及失效模式。结果:环形聚酰胺缝线相当于单丝聚丙烯使用凯斯勒核模式。与单独使用芯线相比,使用运行ES增强的环形聚酰胺缝合线可显著提高产量、峰值和失效载荷,分别提高3.2倍、3.0倍和2.6倍。使用ES需要更大的力来形成3mm的间隙,同时减少测试结构中间隙的发生。不同实验构式的失败模式不同。结论:环式聚酰胺缝合线与单丝聚丙烯缝合线相同。我们的研究结果支持添加ES增强,这是一种简单的技术修改,可以显着提高修复强度,同时减少肌腱末端之间间隙的发生。未来有必要进行体内研究,研究缝线放置对肌腱愈合、血液供应和滑动功能的影响。
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引用次数: 1
The Microbiology of Cheese and Dairy Products is a Critical Step in Ensuring Health, Quality and Typicity 奶酪和乳制品的微生物学是确保健康、质量和典型的关键步骤
Pub Date : 2022-07-25 DOI: 10.54026/cjdvs1043
S. Focardi
Cheese and dairy products require a rigorous observation of the procedures, strictly linked to the different indigenous components, such as the initial raw materials, the process chain, the ripening temperature, the water activity (aw), the pH and the contamination of the environment and operators. Microorganisms are key agents in the transformation of milk and in the subsequent phases which confer typicity and stability to cheese and dairy products. Contamination by pathogenic microorganisms may occur, compromising the safety of the final products. Meanwhile, beneficial microorganisms present in cheese and dairy products can produce antimicrobial compounds, thus avoiding spoilage of the products, ensuring their safety for human consumption. This mini review reports a description of the microorganisms involved in the fermentation of milk and in the subsequent processes concerning cheeses and derivatives, highlighting the aspects that microorganisms play in terms of quality, typicality and safety. New aspects emerged from this study, suggesting possible insights and future research. These include cultural approaches on the one hand, which allow for the isolation and characterization of new microbial strains that confer peculiarities in terms of quality and typicality to cheeses and dairy products, and the isolation of lactic acid bacteria that produce bacteriocins as important tools for combating microbial pathogens. On the other hand, investigations on cheese and dairy products using metagenomic approaches with DNA extraction followed by amplification and sequencing of microbial genes, allow the description and monitoring of the entire microbiota involved in the transformation processes of cheese and dairy products. Therefore, the combination of cultural dairy microbiology and metagenomic approaches can lead to improving the characteristics of cheeses and dairy products, while maintaining respect for traditions.
奶酪和乳制品需要严格观察生产过程,严格与不同的本土成分联系起来,如初始原料、工艺链、成熟温度、水活度(aw)、pH值以及环境和操作人员的污染。微生物是牛奶转化的关键因素,并在随后的阶段赋予奶酪和乳制品的典型性和稳定性。可能会发生病原微生物污染,危及最终产品的安全性。同时,奶酪和乳制品中存在的有益微生物可以产生抗菌化合物,从而避免产品变质,确保人类消费的安全性。这篇小型综述报告了参与牛奶发酵和随后的奶酪及其衍生物过程的微生物,强调了微生物在质量、典型性和安全性方面的作用。从这项研究中出现了新的方面,提出了可能的见解和未来的研究。一方面,这些方法包括培养方法,它允许分离和表征新的微生物菌株,这些菌株赋予奶酪和乳制品在质量和典型性方面的特殊性,以及分离产生细菌素的乳酸菌,这些细菌素是对抗微生物病原体的重要工具。另一方面,利用宏基因组方法对奶酪和乳制品进行研究,提取DNA,然后对微生物基因进行扩增和测序,可以描述和监测参与奶酪和乳制品转化过程的整个微生物群。因此,将培养乳制品微生物学和宏基因组学方法相结合,可以改善奶酪和乳制品的特性,同时保持对传统的尊重。
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引用次数: 1
Livestock Development for Benefit of Small Farmers: Critical Components for Success 畜牧业发展有利于小农:成功的关键因素
Pub Date : 2022-07-04 DOI: 10.54026/cjdvs1042
Narayan G. Hegde
1310 kg per lactation. This reflects on the superior genetic base and efficient management systems in the developed countries, where the aim is to produce more milk with lesser number of cattle. Hence, the challenge is to increase profitability, through increase in milk yield, while reducing the cost of milk production and the herd size.
每次哺乳期1310公斤。这反映了发达国家优越的遗传基础和有效的管理系统,这些国家的目标是用较少的牛生产更多的牛奶。因此,挑战在于通过提高产奶量来提高盈利能力,同时降低产奶成本和畜群规模。
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引用次数: 0
An Insight on Feed Formulation for Livestock 畜禽饲料配方研究进展
Pub Date : 2022-05-30 DOI: 10.54026/cjdvs1041
Radha Gupta
Animal diets now a days contain a wide range of plant-based materials, as well as rendered animals and animal waste, antibiotics, and vitamins. Various feed components are utilised in the meal, depending on the animal. Animals will need to be supplemented during late pregnancy, early lactation, or rapid growth to ensure that requirements are satisfied. The required nutrients by each animal differs according on body weight, milk output, milk fat, and other factors. As an outcome, the feed formulation tool can now be developed using a variety of unique ration formulation methods, such as linear programming, stochastic programming, and goal programming. Many programmes exist that create rations for various livestock species using the most cost-effective feeds available. It will also help the farmer choose feeds that fulfil the ration’s nutritional needs. ICAR-NIANP Smart Tools, Dairy cattle nutrition and feed calculator, Application for Least-Cost Ration Formulation, and Feed Chart are only a few examples.
现在的动物饮食中含有各种各样的植物性材料,以及人造动物和动物粪便、抗生素和维生素。根据动物的不同,在饲料中使用不同的饲料成分。动物在妊娠晚期、哺乳期早期或快速生长期间需要补充,以确保满足需求。每只动物所需的营养根据体重、产奶量、乳脂和其他因素而有所不同。因此,现在可以使用各种独特的饲料配方方法来开发饲料配方工具,例如线性规划,随机规划和目标规划。目前有许多方案利用最具成本效益的饲料为各种牲畜创造口粮。它还将帮助农民选择满足口粮营养需求的饲料。ICAR-NIANP智能工具、奶牛营养与饲料计算器、最低成本日粮配方应用程序、饲料图表等只是其中的几个例子。
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引用次数: 0
期刊
Corpus Journal of Dairy and Veterinary Science (CJDVS)
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