Pub Date : 2024-05-09DOI: 10.2174/0115734072295011240425080837
Tat’yana Khonina, Alexander Safronov, M. Bulatova, L. Larionov, Irina Antropova, M. Dobrinskaya, N. Izmozherova, D. Tishin, Semyon Alekseenko, E. Bogdanova, Maxim Karabanalov, O. Chupakhin
Glycerolates of biogenic elements are of interest because of their pharmacological activity. Some of them are used as active substances in agents for topical application and as biocompatible precursors in sol-gel synthesis of bioactive materials. In this work, morphostructural feature, ability to hydrolyze, and the pharmacological activity of previously synthesized iron(III) monoglycerate were studied. Analytical techniques, including SEM, TEM, XRD, TGA, IR spectroscopy, DLS and ELS, were used. Hemostatic activity was studied in vivo, and primary toxicological studies were carried out on experimental animals. Antimicrobial activity was studied using the agar diffusion method. When dispersed in glycerol, solid crystalline iron(III) monoglycerolate transforms into an amorphous state, forming aggregates with an average particle size of 250 nm (according to DLS data). It slowly hydrolyzes in water at room temperature, while hydrolysis does not take place in an aqueous glycerol media. Iron(III) monoglycerolate is nontoxic and exhibits pronounced hemostatic activity and low antibacterial activity (relative to the strain S. аureus). Iron(III) monoglycerolate can be considered a potential hemostatic agent, showing promise for topical application in medical and veterinary practice, as well as a novel biocompatible precursor in the sol-gel synthesis of practically useful substances.
{"title":"Iron(III) Monoglycerolate: A Potential Hemostatic Agent for Topical Application","authors":"Tat’yana Khonina, Alexander Safronov, M. Bulatova, L. Larionov, Irina Antropova, M. Dobrinskaya, N. Izmozherova, D. Tishin, Semyon Alekseenko, E. Bogdanova, Maxim Karabanalov, O. Chupakhin","doi":"10.2174/0115734072295011240425080837","DOIUrl":"https://doi.org/10.2174/0115734072295011240425080837","url":null,"abstract":"\u0000\u0000Glycerolates of biogenic elements are of interest because of their pharmacological\u0000activity. Some of them are used as active substances in agents for topical application\u0000and as biocompatible precursors in sol-gel synthesis of bioactive materials.\u0000\u0000\u0000\u0000In this work, morphostructural feature, ability to hydrolyze, and the pharmacological\u0000activity of previously synthesized iron(III) monoglycerate were studied.\u0000\u0000\u0000\u0000Analytical techniques, including SEM, TEM, XRD, TGA, IR spectroscopy, DLS and\u0000ELS, were used. Hemostatic activity was studied in vivo, and primary toxicological studies were\u0000carried out on experimental animals. Antimicrobial activity was studied using the agar diffusion\u0000method.\u0000\u0000\u0000\u0000When dispersed in glycerol, solid crystalline iron(III) monoglycerolate transforms into\u0000an amorphous state, forming aggregates with an average particle size of 250 nm (according to\u0000DLS data). It slowly hydrolyzes in water at room temperature, while hydrolysis does not take\u0000place in an aqueous glycerol media. Iron(III) monoglycerolate is nontoxic and exhibits pronounced\u0000hemostatic activity and low antibacterial activity (relative to the strain S. аureus).\u0000\u0000\u0000\u0000Iron(III) monoglycerolate can be considered a potential hemostatic agent, showing\u0000promise for topical application in medical and veterinary practice, as well as a novel biocompatible\u0000precursor in the sol-gel synthesis of practically useful substances.\u0000","PeriodicalId":10772,"journal":{"name":"Current Bioactive Compounds","volume":" 10","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140996530","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-04-24DOI: 10.2174/0115734072296835240409124751
S. Sruthi, Shahjahan A, Kumaran K, Dannie Macrin, Vijay Murali RM, K.N. Aruljothi
Marine algae stand out as repositories of bioactive metabolites, widely harnessed in treating diverse diseases. Given the often-challenging side effects associated with conventional cancer treatments, researchers are increasingly turning their attention to marine algae as a promising source for discovering novel and potent anticancer compounds. In this study, we meticulously explored the potential anticancer activity of Spatoglossum variabile and Gracilaria corticata sourced from the Indian Ocean. Marine algae are rich in bioactive metabolites that are extensively used to treat various diseases. Researchers have considered marine algae for novel and promising anticancer compounds due to the adverse effects of standard cancer treatments The secondary metabolites were extracted using methanol solvent according to the standard protocol. The extract was subjected to GC-MS analysis and evaluated for its short- and long-term cytotoxicity and anti-proliferative ability in the HCT116 cell line using MTT, clonogenic, and trypan blue dye exclusion assays, respectively. The methanolic extract of Spatoglossum variabile contained 21 unique anticancer compounds, except for the other bioactive compounds. The results indicate that increasing the concentration of algal extracts decreased the cell viability significantly, with an IC50 of 51.49 μg/mL and 37.50 μg /mL in the HCT116 cell line. The results showed a significant reduction in colorectal cancer colony formation and a gradual reduction in cell proliferation. These results underscore the potential for targeted, low-toxicity biomedicines due to their selective cytotoxicity against cancer cells. The extract was subjected to GC-MS analysis and evaluated for its short- and long-term cytotoxicity and anti-proliferative ability in HCT116 cell line using MTT, clonogenic, and trypan blue dye exclusion assays, respectively. Our findings show that Spatoglosuum variabile & Gracilaria corticata extracts contain various bioactive compounds, display long-term and short-term cytotoxicity and antiproliferative effects, which indicates that their constituent compounds might be further refined into effective anti-colorectal cancer medications. The methanolic extract of Spatoglossum variabile contained 21 unique anti-cancer compounds, in addition to other bioactive compounds. The results indicate that increasing the concentration of algal extracts decreased the cell viability significantly, with an IC50 of 51.49 µg/mL and 37.50 µg /ml in the HCT116 cell line. The results showed a significant reduction in colony formation and a gradual reduction in cell proliferation
{"title":"Anticancer Activity and GC-MS Profiling of Bioactive Constituents in the Methanolic Extracts of Spatoglossum variabile and Gracilaria corticata","authors":"S. Sruthi, Shahjahan A, Kumaran K, Dannie Macrin, Vijay Murali RM, K.N. Aruljothi","doi":"10.2174/0115734072296835240409124751","DOIUrl":"https://doi.org/10.2174/0115734072296835240409124751","url":null,"abstract":"\u0000\u0000Marine algae stand out as repositories of bioactive metabolites, widely\u0000harnessed in treating diverse diseases. Given the often-challenging side effects associated with\u0000conventional cancer treatments, researchers are increasingly turning their attention to marine algae\u0000as a promising source for discovering novel and potent anticancer compounds. In this study,\u0000we meticulously explored the potential anticancer activity of Spatoglossum variabile and\u0000Gracilaria corticata sourced from the Indian Ocean.\u0000\u0000\u0000\u0000Marine algae are rich in bioactive metabolites that are extensively used to treat various diseases. Researchers have considered marine algae for novel and promising anticancer compounds due to the adverse effects of standard cancer treatments\u0000\u0000\u0000\u0000The secondary metabolites were extracted using methanol solvent according\u0000to the standard protocol. The extract was subjected to GC-MS analysis and evaluated for\u0000its short- and long-term cytotoxicity and anti-proliferative ability in the HCT116 cell line using\u0000MTT, clonogenic, and trypan blue dye exclusion assays, respectively.\u0000\u0000\u0000\u0000The methanolic extract of Spatoglossum variabile contained 21 unique\u0000anticancer compounds, except for the other bioactive compounds. The results indicate that increasing\u0000the concentration of algal extracts decreased the cell viability significantly, with an IC50\u0000of 51.49 μg/mL and 37.50 μg /mL in the HCT116 cell line. The results showed a significant reduction\u0000in colorectal cancer colony formation and a gradual reduction in cell proliferation. These\u0000results underscore the potential for targeted, low-toxicity biomedicines due to their selective cytotoxicity\u0000against cancer cells.\u0000\u0000\u0000\u0000The extract was subjected to GC-MS analysis and evaluated for its short- and long-term cytotoxicity and anti-proliferative ability in HCT116 cell line using MTT, clonogenic, and trypan blue dye exclusion assays, respectively.\u0000\u0000\u0000\u0000Our findings show that Spatoglosuum variabile & Gracilaria corticata extracts contain\u0000various bioactive compounds, display long-term and short-term cytotoxicity and antiproliferative\u0000effects, which indicates that their constituent compounds might be further refined\u0000into effective anti-colorectal cancer medications.\u0000\u0000\u0000\u0000The methanolic extract of Spatoglossum variabile contained 21 unique anti-cancer compounds, in addition to other bioactive compounds. The results indicate that increasing the concentration of algal extracts decreased the cell viability significantly, with an IC50 of 51.49 µg/mL and 37.50 µg /ml in the HCT116 cell line. The results showed a significant reduction in colony formation and a gradual reduction in cell proliferation\u0000","PeriodicalId":10772,"journal":{"name":"Current Bioactive Compounds","volume":"63 40","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-04-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140664067","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-04-18DOI: 10.2174/0115734072278162240406123303
Anuradha Mehra, Rekha Sangwan, Emmanuel Owusu
Xanthones are a class of naturally occurring compounds found in various plant sources. Over the years, studies have revealed therapeutic impending xanthones in treating and managing several ailments, including cancer, inflammation, and microbial infections. Xanthones exert their therapeutic effects through various mechanisms, such as anti-inflammatory, antioxidant, and anti-proliferative activities. It has also been found that xanthones induce apoptosis, inhibit cell proliferation, and arrest cell cycles by modulating several signaling pathways. This review objective is to deliver an inclusive overview of the therapeutic essence of xanthones and their potential use as a natural remedy for managing and treating various diseases.
{"title":"Xanthone Derivatives: A Pharmacological Panorama of Versatility","authors":"Anuradha Mehra, Rekha Sangwan, Emmanuel Owusu","doi":"10.2174/0115734072278162240406123303","DOIUrl":"https://doi.org/10.2174/0115734072278162240406123303","url":null,"abstract":"\u0000\u0000Xanthones are a class of naturally occurring compounds found in various plant\u0000sources. Over the years, studies have revealed therapeutic impending xanthones in treating and\u0000managing several ailments, including cancer, inflammation, and microbial infections. Xanthones\u0000exert their therapeutic effects through various mechanisms, such as anti-inflammatory, antioxidant,\u0000and anti-proliferative activities. It has also been found that xanthones induce apoptosis, inhibit\u0000cell proliferation, and arrest cell cycles by modulating several signaling pathways. This review\u0000objective is to deliver an inclusive overview of the therapeutic essence of xanthones and\u0000their potential use as a natural remedy for managing and treating various diseases.\u0000","PeriodicalId":10772,"journal":{"name":"Current Bioactive Compounds","volume":" 3","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-04-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140689828","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-04-18DOI: 10.2174/0115734072285993240403110533
U. D'Souza, Chandrashekar K.S., Shruthi R, Vasudev Pai
Curculigo orchioides Gaertn, a plant belonging to the Hypoxidaceae family, is well-known for its traditional uses of all plant parts. This plant is commonly known as golden eye grass, black musli, Kali musli, or Kali Musali. Parkinson's disease is a neurodegenerative disorder that is commonly observed in older adults. It is caused by a variety of mechanisms, including neuroinflammation, which results in the loss of dopamine release when triggered and causes motor symptoms. Ethanolic extract was prepared by shade-drying the leaves, making them into a coarse powder, and the extract was prepared by maceration technique with the help of ethanol. GCMS analysis and Haloperidol-induced Parkinsonism study were carried out to evaluate the antiParkinson activity in albino Wistar rats. Ethanolic extract of Curculigo orchioides was subjected to GCMS analysis. The National Institute of Standards and Technology database was used to interpret the mass spectrum of the GC-MS. The screening models were catalepsy, rotarod and a few in vitro models, namely the estimation of catalase acetylcholinesterase levels. The animals were divided into 6 groups, namely control, disease, standard (levodopa), low dose (100 mg/kg p.o.), medium dose (200 mg/kg p.o.), high dose (400 mg/kg p.o.) groups having 6 animals each were used for rotarod and catalepsy models for 14 days The results obtained showed a dose-dependent increase in the anti-Parkinson activity. The evaluation of neuromuscular movements of the ethanolic leaf extract of Curculigo orchioides showed that the pre-treatment with different ranges of dose (100 mg/ml, 200 mg/kg, and 400 mg/kg) suggested a dose-dependent significant increase in fall-off time when compared with both standard and disease groups GCMS analysis revealed the presence of flavonoids, Vitamin and fatty acid esters. The anti-Parkinson activity of Flavonoids has been reported previously. Hence, the activity may be attributed to kaempferol-3-O-glucuronide.
{"title":"GCMS Analysis of Bioactive Compounds and Evaluation of Anti-parkinson\u0000Activity of Leaf Extract of Curculigo orchioides in Experimental Animals","authors":"U. D'Souza, Chandrashekar K.S., Shruthi R, Vasudev Pai","doi":"10.2174/0115734072285993240403110533","DOIUrl":"https://doi.org/10.2174/0115734072285993240403110533","url":null,"abstract":"\u0000\u0000Curculigo orchioides Gaertn, a plant belonging to the Hypoxidaceae\u0000family, is well-known for its traditional uses of all plant parts. This plant is commonly known\u0000as golden eye grass, black musli, Kali musli, or Kali Musali. Parkinson's disease is a neurodegenerative disorder that is commonly observed in older adults. It is caused by a variety of mechanisms, including neuroinflammation, which results in the loss of dopamine release when triggered\u0000and causes motor symptoms.\u0000\u0000\u0000\u0000Ethanolic extract was prepared by shade-drying the leaves, making them into a coarse\u0000powder, and the extract was prepared by maceration technique with the help of ethanol. GCMS\u0000analysis and Haloperidol-induced Parkinsonism study were carried out to evaluate the antiParkinson activity in albino Wistar rats. Ethanolic extract of Curculigo orchioides was subjected\u0000to GCMS analysis. The National Institute of Standards and Technology database was used to interpret the mass spectrum of the GC-MS. The screening models were catalepsy, rotarod and a few\u0000in vitro models, namely the estimation of catalase acetylcholinesterase levels. The animals were\u0000divided into 6 groups, namely control, disease, standard (levodopa), low dose (100 mg/kg p.o.),\u0000medium dose (200 mg/kg p.o.), high dose (400 mg/kg p.o.) groups having 6 animals each were\u0000used for rotarod and catalepsy models for 14 days\u0000\u0000\u0000\u0000The results obtained showed a dose-dependent increase in the anti-Parkinson activity.\u0000The evaluation of neuromuscular movements of the ethanolic leaf extract of Curculigo orchioides\u0000showed that the pre-treatment with different ranges of dose (100 mg/ml, 200 mg/kg, and 400\u0000mg/kg) suggested a dose-dependent significant increase in fall-off time when compared with both\u0000standard and disease groups GCMS analysis revealed the presence of flavonoids, Vitamin and\u0000fatty acid esters.\u0000\u0000\u0000\u0000The anti-Parkinson activity of Flavonoids has been reported previously. Hence, the\u0000activity may be attributed to kaempferol-3-O-glucuronide.\u0000","PeriodicalId":10772,"journal":{"name":"Current Bioactive Compounds","volume":" 10","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-04-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140690059","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-04-16DOI: 10.2174/0115734072297928240404074703
P. Shakib, Shirzad Fallahi, G. Goudarzi, Shahram Ahmadi Somaghian, Sana Amiri, Sirus Nemati Delfan, Kourosh Cheraghipour
This investigation aims to explore the potential anti-Candida albicans effects of Chitosan nanocomposites. Researchers searched several databases, including Scopus, PubMed, Science Direct, Cochrane, and Ovid, for articles published between 2000 and 2023 using specific keywords. Of the 5,600 studies that were first searched in the databases, after removing duplicates (700 cases) and applying inclusion and exclusion criteria, 43 studies were selected for evaluation after duplicate removal. According to studies, the anti-Candida albicans properties of chitosan nanocomposites are more than routine antifungals such as nystatin; therefore, it can be concluded that Chitosan nanocomposites can be used to deal with Candida albicans.
{"title":"Antifungal Activity of Chitosan Nanocomposites against Candida albicans:\u0000A Systematic Review","authors":"P. Shakib, Shirzad Fallahi, G. Goudarzi, Shahram Ahmadi Somaghian, Sana Amiri, Sirus Nemati Delfan, Kourosh Cheraghipour","doi":"10.2174/0115734072297928240404074703","DOIUrl":"https://doi.org/10.2174/0115734072297928240404074703","url":null,"abstract":"\u0000\u0000This investigation aims to explore the potential anti-Candida albicans\u0000effects of Chitosan nanocomposites.\u0000\u0000\u0000\u0000Researchers searched several databases, including Scopus, PubMed, Science Direct,\u0000Cochrane, and Ovid, for articles published between 2000 and 2023 using specific keywords.\u0000\u0000\u0000\u0000Of the 5,600 studies that were first searched in the databases, after removing duplicates\u0000(700 cases) and applying inclusion and exclusion criteria, 43 studies were selected for evaluation\u0000after duplicate removal.\u0000\u0000\u0000\u0000According to studies, the anti-Candida albicans properties of chitosan nanocomposites\u0000are more than routine antifungals such as nystatin; therefore, it can be concluded that Chitosan\u0000nanocomposites can be used to deal with Candida albicans.\u0000","PeriodicalId":10772,"journal":{"name":"Current Bioactive Compounds","volume":"62 1‐2","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-04-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140695453","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-04-16DOI: 10.2174/0115734072298465240403084903
Dilipkumar S, Karthik V, Gowramma B, Magesh M, Kaviarasan L
Many quinone derivatives as of now utilized for anticancer medications. Especially, 1,4-naphthoquinones are dynamic derivatives, and it was broadly utilized in unrefined substances in the drugs and agrochemicals industry. In this work, we planned and combined five different moieties into 2, 3 disubstituted naphthalene-1,4-dione molecules. Various spectral studies distinguished the synthetic designs of the produced compounds. The naphthoquinone derivatives were exposed to the primary molecular descriptor by Molinspiration programming, and all the descriptor values are within the specified value. In this work, we planned and combined five different moieties into 2, 3 disubstituted naphthalene-1,4-dione molecules. Various spectral studies distinguished the synthetic designs of the produced compounds. The naphthoquinone derivatives were exposed to the primary molecular descriptor by Molinspiration programming, and all the descriptor values are within the specified value. Each of the five naphthoquinone derivatives was docked against the Topoisomerase II utilizing Auto Dock program 4.2.5. (PDB: 3L4K). The docking tells us that the studied compounds possess significant to moderate inhibition toward the targeted enzymes. Among the studied compounds, compound L3 showed the most elevated binding score (-10.66 kcal/mol with one H-bond) than the adriamycin (-9.58 kcal/mol with two H-bonds) and compound L2 (- 9.86 kcal/mol with two H-bonds). The derivatives were tried for in-vitro cytotoxicity studies against MCF - 7 by the SRB method. Among them, compounds L2 (28.42±3.1 μg/mL) and L3 (29.38±3.2 μg/mL) were the most significant ones when contrasted with the control Adriamycin (15.28±3.4 μg/mL). The current research indicates that the tested compounds show anticancer action against the MCF-7 breast cancer cell line. Thus, the study is an attempt to advance toward the identification of innovative anticancer drugs.
目前，许多醌类衍生物已被用于抗癌药物，尤其是 1,4-萘醌类衍生物具有很强的生命力，在药物和农用化学品行业中被广泛应用于未精制物质。各种光谱研究区分了所制化合物的合成设计。通过 Molinspiration 程序对萘醌衍生物进行了主分子描述符分析，所有描述符值均在规定值范围内。各种光谱研究区分了所生成化合物的合成设计。利用 Molinspiration 程序对萘醌衍生物进行了主分子描述，所有描述值均在指定值范围内。(PDB：3L4K）与拓扑异构酶 II 进行对接。在所研究的化合物中，化合物 L3 的结合得分最高（-10.66kcal/mol，一个 H 键），高于阿霉素（-9.58 kcal/mol，两个 H 键）和化合物 L2（-9.86 kcal/mol，两个 H 键）。采用 SRB 法对这些衍生物进行了针对 MCF - 7 的体外细胞毒性研究。与对照组阿霉素（15.28±3.4 μg/mL）相比，其中化合物 L2（28.42±3.1 μg/mL）和 L3（29.38±3.2 μg/mL）的细胞毒性最为显著。本研究表明，测试化合物对 MCF-7 乳腺癌细胞株具有抗癌作用。
{"title":"Synthesis, Characterization, Docking Studies, and In-vitro Cytotoxic\u0000Activity of Some Novel 2, 3 Disubstituted Naphthalene 1,4 Dione\u0000Derivatives","authors":"Dilipkumar S, Karthik V, Gowramma B, Magesh M, Kaviarasan L","doi":"10.2174/0115734072298465240403084903","DOIUrl":"https://doi.org/10.2174/0115734072298465240403084903","url":null,"abstract":"\u0000\u0000Many quinone derivatives as of now utilized for anticancer medications.\u0000Especially, 1,4-naphthoquinones are dynamic derivatives, and it was broadly utilized in unrefined\u0000substances in the drugs and agrochemicals industry.\u0000\u0000\u0000\u0000In this work, we planned and combined five different moieties into 2, 3\u0000disubstituted naphthalene-1,4-dione molecules. Various spectral studies distinguished the synthetic\u0000designs of the produced compounds. The naphthoquinone derivatives were exposed to the primary\u0000molecular descriptor by Molinspiration programming, and all the descriptor values are within\u0000the specified value.\u0000\u0000\u0000\u0000In this work, we planned and combined five different moieties into 2, 3\u0000disubstituted naphthalene-1,4-dione molecules. Various spectral studies distinguished the synthetic\u0000designs of the produced compounds. The naphthoquinone derivatives were exposed to the primary\u0000molecular descriptor by Molinspiration programming, and all the descriptor values are within\u0000the specified value.\u0000\u0000\u0000\u0000Each of the five naphthoquinone derivatives was docked against the\u0000Topoisomerase II utilizing Auto Dock program 4.2.5. (PDB: 3L4K). The docking tells us that the\u0000studied compounds possess significant to moderate inhibition toward the targeted enzymes.\u0000Among the studied compounds, compound L3 showed the most elevated binding score (-10.66\u0000kcal/mol with one H-bond) than the adriamycin (-9.58 kcal/mol with two H-bonds) and compound\u0000L2 (- 9.86 kcal/mol with two H-bonds). The derivatives were tried for in-vitro cytotoxicity\u0000studies against MCF - 7 by the SRB method. Among them, compounds L2 (28.42±3.1 μg/mL)\u0000and L3 (29.38±3.2 μg/mL) were the most significant ones when contrasted with the control Adriamycin\u0000(15.28±3.4 μg/mL).\u0000\u0000\u0000\u0000The current research indicates that the tested compounds show anticancer action\u0000against the MCF-7 breast cancer cell line. Thus, the study is an attempt to advance toward the\u0000identification of innovative anticancer drugs.\u0000","PeriodicalId":10772,"journal":{"name":"Current Bioactive Compounds","volume":"1 5","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-04-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140697708","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-04-09DOI: 10.2174/0115734072282302240402043805
Soukaina Laaraj, Ibtisaam Ouahidi, Nada Al Moudani, A. Boukir, Samira Jaouhar, Safae Er Raouan, L. Aarab
In the past few decades, researchers have focused on finding the benefits of natural substances derived from plants. This study aimed to evaluate the use of Brassica rapa seeds in poultry feed as an antioxidant and immunostimulant of host defenses. We prepared three extracts using ethanol, Ethyl Acetate, and water. Spectrophotometric methods determined the total phenolic and flavonoid content in the three extracts. Antioxidant activity was assessed using 1,1-diphenyl-2-picrylhydrazyl (DPPH), ferric reducing/antioxidant power (FRAP), 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS) radicals and β-Carotene bleaching methods. An assessment of their immunostimulant activities in vitro was performed on chicken immunity cell proliferation (splenocytes, thymocytes, and Bursa cells) and IgY production. We prepared three different extracts using ethanol, Ethyl Acetate, and water. The total phenolic and flavonoid content in the three extracts was determined by spectrophotometric methods.Antioxidant activity has been assessed using 1,1-diphenyl-2-picrylhydrazyl (DPPH), ferric reducing/antioxidant power (FRAP), 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS) radicals and β-Carotene bleaching methods. Assessment of their immunostimulant activities in vitro was tested on chicken immunity cell proliferation (splenocytes, thymocytes, and Bursa cells) and IgY production The total phenolic contents ranged from 462.5 to 794.8 mg /g of extract, the order of TPC was as follows: EtOAc > EtOH >Water. Flavonoid contents were, 63.7 mg in EtOH extract, 81.2 mg in EtOAc extract, and 108.7 mg/g Extract in aqueous extract. By DPPH, the IC50s of EtOH, EtOAc, and water, were 1.8μg/ml, 2.4μg/ml, and 1.5μg/ml, respectively. Using the Ferric- Reducing Antioxidant Power (FRAP), we remarked that the EtOH and EtOAc extracts have important antioxidant powers. The ABTS assay indicated that EtOH and water had the highest activities with IC50s of 0.19 and 0.07, respectively. Finally, by the β-carotene bleaching test, we observed that the IC50 of the EtOH, EtOAc, and water were 62.1 μg/mL; 72.7 μg/mL, and 45.8 μg/mL, respectively. The results obtained indicate that Brassica aqueous extract stimulates humoral immunity by stimulating splenocyte (B and T-lymphocytes) and Bursa cell (B-lymphocyte) proliferation by more than 200% of response vs control. In addition, the aqueous extract highly stimulated the function of bursa cells by 208% of the reaction. In the same conditions, we recorded a stimulation of cellular immunity mediated by thymocytes by an increase in cell proliferation (352.7% of response) implicated in virus protection. These extracts also possessed an antimicrobial effect against diverse microorganisms such as coliforms and Staphylococcus. The FT-IR spectrum indicated that the hydroxyl group (phenol), hydroxybenzoic acid family, carbohydrate molecules such as glucopyranose, a carbonyl ester group, hydrocar
{"title":"Antioxidant Properties of Brassica Rapa L. Seeds and Immunostimulant Effect on Immunity Broilers Cells (Gallus Gallus Domesticus)","authors":"Soukaina Laaraj, Ibtisaam Ouahidi, Nada Al Moudani, A. Boukir, Samira Jaouhar, Safae Er Raouan, L. Aarab","doi":"10.2174/0115734072282302240402043805","DOIUrl":"https://doi.org/10.2174/0115734072282302240402043805","url":null,"abstract":"\u0000\u0000In the past few decades, researchers have focused on finding the benefits\u0000of natural substances derived from plants.\u0000\u0000\u0000\u0000This study aimed to evaluate the use of Brassica rapa seeds in poultry feed as an antioxidant\u0000and immunostimulant of host defenses.\u0000\u0000\u0000\u0000We prepared three extracts using ethanol, Ethyl Acetate, and water. Spectrophotometric\u0000methods determined the total phenolic and flavonoid content in the three extracts. Antioxidant\u0000activity was assessed using 1,1-diphenyl-2-picrylhydrazyl (DPPH), ferric reducing/antioxidant\u0000power (FRAP), 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS)\u0000radicals and β-Carotene bleaching methods. An assessment of their immunostimulant activities in\u0000vitro was performed on chicken immunity cell proliferation (splenocytes, thymocytes, and Bursa\u0000cells) and IgY production.\u0000\u0000\u0000\u0000We prepared three different extracts using ethanol, Ethyl Acetate, and water. The total phenolic and flavonoid content in the three extracts was determined by spectrophotometric methods.Antioxidant activity has been assessed using 1,1-diphenyl-2-picrylhydrazyl (DPPH), ferric reducing/antioxidant power (FRAP), 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS) radicals and β-Carotene bleaching methods. Assessment of their immunostimulant activities in vitro was tested on chicken immunity cell proliferation (splenocytes, thymocytes, and Bursa cells) and IgY production\u0000\u0000\u0000\u0000The total phenolic contents ranged from 462.5 to 794.8 mg /g of extract, the order of\u0000TPC was as follows: EtOAc > EtOH >Water. Flavonoid contents were, 63.7 mg in EtOH extract,\u000081.2 mg in EtOAc extract, and 108.7 mg/g Extract in aqueous extract. By DPPH, the IC50s of\u0000EtOH, EtOAc, and water, were 1.8μg/ml, 2.4μg/ml, and 1.5μg/ml, respectively. Using the Ferric-\u0000Reducing Antioxidant Power (FRAP), we remarked that the EtOH and EtOAc extracts have important\u0000antioxidant powers. The ABTS assay indicated that EtOH and water had the highest activities\u0000with IC50s of 0.19 and 0.07, respectively. Finally, by the β-carotene bleaching test, we observed\u0000that the IC50 of the EtOH, EtOAc, and water were 62.1 μg/mL; 72.7 μg/mL, and 45.8\u0000μg/mL, respectively. The results obtained indicate that Brassica aqueous extract stimulates humoral\u0000immunity by stimulating splenocyte (B and T-lymphocytes) and Bursa cell (B-lymphocyte)\u0000proliferation by more than 200% of response vs control. In addition, the aqueous extract highly\u0000stimulated the function of bursa cells by 208% of the reaction. In the same conditions, we recorded\u0000a stimulation of cellular immunity mediated by thymocytes by an increase in cell proliferation\u0000(352.7% of response) implicated in virus protection. These extracts also possessed an antimicrobial\u0000effect against diverse microorganisms such as coliforms and Staphylococcus. The FT-IR\u0000spectrum indicated that the hydroxyl group (phenol), hydroxybenzoic acid family, carbohydrate\u0000molecules such as glucopyranose, a carbonyl ester group, hydrocar","PeriodicalId":10772,"journal":{"name":"Current Bioactive Compounds","volume":"45 4","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-04-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140721616","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-04-09DOI: 10.2174/0115734072286081240326082701
K. Patel, D. Patel
Natural products and their derived secondary metabolites play an important role in medicine and other allied health sectors. Liriodendrin is one of the main lignans of Boerhaavia diffusa roots. Liriodendrin is a lignin class phytochemical having numerous health beneficial properties in medicine, including anti-myocardial ischemia, anti-arrhythmic, anti-oxidant and anti- inflammatory potential. This present review aims to analyze the reported ethnomedicinal properties, pharmacological activities and analytical aspects of liriodendrin and identify the remaining gaps in medicinal fields for their future investigations. The pharmacological properties of liriodendrin have been described in the present paper in order to describe its better utilization in natural medicine in the future. Biological poptential and pharmacological activities of liriodendrin have been described in the present work with their analytical development in the scientific fields. In the present work, scientific data on liriodendrin were collected from different scientific databases such as PubMed, Springer, Google, Science Direct, and Google Scholar. They described their biological potential on the basis of the available scientific literature. Further, research and review articles from peer-reviewed journals were also searched during data collection. The present paper's scientific data signified the biological importance of liriodendrin in medicine, which has been isolated from Boerhaavia diffusa and other medicinal plants. Liriodendrin has biological potential against myocardial infarction, arrhythmias, lung injury, hepatic injury, antiinflammatory, ulcerative colitis, gastric injury, SARS-CoV-2 protease and intestinal inflammation. Further, its effectiveness in medicine was also due to its anti-convulsant activity, nitric oxide inhibitory potential, cytotoxicity, cytoprotective nature, anti-oxidant, and anti-microbial potential. The present paper's scientific data also described the metabolism and analytical development for their isolation, separation and identification in different samples. Detailed pharmacological activities of liriodendrin have been described here in the present work and highlighted its important pharmacological properties and analytical aspects. Liriodendrin is a valuable phytochemical of Boerhaavia diffusa, which has numerous biological applications in medicine. Further, this study can also validate the traditional and ethnobotanical use of the Boerhaavia diffusa and other plant material, which contain a significant amount of liriodendrin as an active phytochemical. Liriodendrin have huge biological potential far beyond its traditional uses in human complications.
天然产品及其衍生的次级代谢物在医药和其他相关保健领域发挥着重要作用。鹅掌楸木质素是鹅掌楸根中的主要木质素之一。鹅掌楸木质素是一种木质素类植物化学物质，在医学上具有多种有益健康的特性，包括抗心肌缺血、抗心律失常、抗氧化和抗炎潜力。本综述旨在分析所报道的鹅掌楸碱的民族药用特性、药理活性和分析方面，并找出在药用领域尚存在的空白，以便今后进行研究。本文阐述了鹅掌楸的药理特性，以便今后在天然药物中更好地利用它。本研究从 PubMed、Springer、Google、Science Direct 和 Google Scholar 等不同的科学数据库中收集了有关鹅掌楸的科学数据。在本研究中，我们从不同的科学数据库（如 PubMer、Springer、Google、Science Direct 和 Google Scholar）中收集了有关鹅掌楸碱的科学数据，并根据现有的科学文献对其生物潜力进行了描述。此外，在数据收集过程中还搜索了同行评审期刊上的研究和评论文章。本文的科学数据表明，从白花蛇舌草和其他药用植物中分离出的鹅掌楸碱在医学上具有重要的生物学意义。鹅掌楸碱具有防治心肌梗塞、心律失常、肺损伤、肝损伤、抗炎、溃疡性结肠炎、胃损伤、SARS-CoV-2 蛋白酶和肠道炎症的生物学潜力。本文的科学数据还描述了它们在不同样品中的代谢和分析发展，以分离、分离和鉴定它们。本研究还详细描述了鹅掌楸碱的药理活性，并着重介绍了其重要的药理特性和分析方法。鹅掌楸中的鹅掌楸碱是一种珍贵的植物化学物质，在医学上有广泛的生物学应用。此外，这项研究还验证了传统和民族植物学中对白花蛇舌草和其他植物材料的使用，这些材料中含有大量的活性植物化学物质--鹅掌楸碱。鹅掌楸具有巨大的生物潜力，远远超出了其在人类并发症中的传统用途。
{"title":"Therapeutic Potential, Pharmacological Activities and Analytical Aspects\u0000of Liriodendrin in Medicine: Novel and Potential Therapy Options for the\u0000Treatment of Human Disorders and Associated Complications","authors":"K. Patel, D. Patel","doi":"10.2174/0115734072286081240326082701","DOIUrl":"https://doi.org/10.2174/0115734072286081240326082701","url":null,"abstract":"\u0000\u0000Natural products and their derived secondary metabolites play an important role in\u0000medicine and other allied health sectors. Liriodendrin is one of the main lignans of Boerhaavia\u0000diffusa roots. Liriodendrin is a lignin class phytochemical having numerous health beneficial\u0000properties in medicine, including anti-myocardial ischemia, anti-arrhythmic, anti-oxidant and anti-\u0000inflammatory potential. This present review aims to analyze the reported ethnomedicinal properties,\u0000pharmacological activities and analytical aspects of liriodendrin and identify the remaining\u0000gaps in medicinal fields for their future investigations. The pharmacological properties of liriodendrin\u0000have been described in the present paper in order to describe its better utilization in natural\u0000medicine in the future. Biological poptential and pharmacological activities of liriodendrin\u0000have been described in the present work with their analytical development in the scientific fields.\u0000In the present work, scientific data on liriodendrin were collected from different scientific databases\u0000such as PubMed, Springer, Google, Science Direct, and Google Scholar. They described\u0000their biological potential on the basis of the available scientific literature. Further, research and\u0000review articles from peer-reviewed journals were also searched during data collection. The present\u0000paper's scientific data signified the biological importance of liriodendrin in medicine, which\u0000has been isolated from Boerhaavia diffusa and other medicinal plants. Liriodendrin has biological\u0000potential against myocardial infarction, arrhythmias, lung injury, hepatic injury, antiinflammatory,\u0000ulcerative colitis, gastric injury, SARS-CoV-2 protease and intestinal inflammation.\u0000Further, its effectiveness in medicine was also due to its anti-convulsant activity, nitric oxide\u0000inhibitory potential, cytotoxicity, cytoprotective nature, anti-oxidant, and anti-microbial potential.\u0000The present paper's scientific data also described the metabolism and analytical development for\u0000their isolation, separation and identification in different samples. Detailed pharmacological activities\u0000of liriodendrin have been described here in the present work and highlighted its important\u0000pharmacological properties and analytical aspects. Liriodendrin is a valuable phytochemical of\u0000Boerhaavia diffusa, which has numerous biological applications in medicine. Further, this study\u0000can also validate the traditional and ethnobotanical use of the Boerhaavia diffusa and other plant\u0000material, which contain a significant amount of liriodendrin as an active phytochemical. Liriodendrin\u0000have huge biological potential far beyond its traditional uses in human complications.\u0000","PeriodicalId":10772,"journal":{"name":"Current Bioactive Compounds","volume":"8 3","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-04-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140720662","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-04-02DOI: 10.2174/0115734072296932240319060406
Kahina Hardou-Belhoucine, F. Brahmi, Daniel Grenier, Khokha Mouhoubi, K. Madani, L. Boulekbache‐Makhlouf
Periodontal disease and dental caries are two oral illnesses that are significantly influenced by microorganisms, hence the usefulness of finding natural substances to inhibit them. This study aimed to assess the Eucalyptus globulus Labill. leaf and fruit essential oils effect against Streptococcus mutans, Streptococcus sobrinus, Aggregatibacter actinomycetemcomitans, Porphyromonas gingivalis, Fusobacterium nucleatum, and Candida albicans to study their anticariogenic and antiperiodonto pathogenic activities. these activities were evaluated according to the determination of minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC), anti-biofilm effects, and the impact on the adhesion to hydroxyapatite, a main component of the tooth. both leaf and fruit essential oils showed strong antibacterial activity against anaerobic bacteria: F. nucleatum (MIC of 0.012 %) and P. gingivalis (MIC of 0.025 %). Anticarcinogenic activity also showed MIC values of 0.06 % against S. mutans and, 0.5 % against S. sobrinus, and appropriate antifungal activity against C. albicans. It is interesting to note that both oils showed a significant inhibitory property to biofilm formation by the different studied species and significantly reduced the adhesion capacity of S. mutans and S. sobrinus to the hydroxyapatite surface at very low concentrations of 0.12 and 0.5 % for S. mutans and S. sobrinus, respectively. These results highlight the strong potential of E. globulus essential oils as antimicrobial and antibiofilm agents, as well as their ability to inhibit bacterial adhesion, which is promising for the prevention of dental caries and plaque. This implies that E. globulus may be a new alternative source of substances of medicinal interest that can be used in the pharmaceutical industry to produce antimicrobial agents against dental caries and oral infectious diseases
牙周病和龋齿是两种受微生物严重影响的口腔疾病，因此寻找天然物质来抑制它们非常有用。本研究旨在评估球叶桉树精油和果实精油对变异链球菌、苏布雷链球菌、放线菌、牙龈卟啉单胞菌、核酸化脓杆菌和白色念珠菌的抗龋齿和抗牙周病致病活性。这些活性是通过测定最低抑菌浓度（MIC）和最低杀菌浓度（MBC）、抗生物膜效应以及对牙齿主要成分羟基磷灰石粘附性的影响来评估的：叶片和果实精油对厌氧菌有很强的抗菌活性：核酸酵母菌（MIC 为 0.012 %）和牙龈球菌（MIC 为 0.025 %）。抗癌活性还显示，对突变酵母菌的 MIC 值为 0.06 %，对索氏酵母菌的 MIC 值为 0.5 %，对白癣菌也有适当的抗真菌活性。值得注意的是，这两种精油对所研究的不同物种形成生物膜都有显著的抑制作用，而且在 0.12% 和 0.5% 的极低浓度下，就能显著降低变异杆菌和索布林杆菌对羟基磷灰石表面的粘附能力。这些结果凸显了球果香精油作为抗菌剂和抗生物膜剂的强大潜力，以及其抑制细菌粘附的能力，这对于预防龋齿和牙菌斑很有前景。这意味着球果香精油可能是一种新的药用物质替代来源，可用于制药业生产抗龋齿和口腔传染病的抗菌剂。
{"title":"Eucalyptus globulus Labill. Essential Oil Effect on the Attachment of Oral\u0000Bacteria to Hydroxyapatite and Biofilm Formation","authors":"Kahina Hardou-Belhoucine, F. Brahmi, Daniel Grenier, Khokha Mouhoubi, K. Madani, L. Boulekbache‐Makhlouf","doi":"10.2174/0115734072296932240319060406","DOIUrl":"https://doi.org/10.2174/0115734072296932240319060406","url":null,"abstract":"\u0000\u0000Periodontal disease and dental caries are two oral illnesses that are significantly\u0000influenced by microorganisms, hence the usefulness of finding natural substances to\u0000inhibit them.\u0000\u0000\u0000\u0000This study aimed to assess the Eucalyptus globulus Labill. leaf and fruit essential oils\u0000effect against Streptococcus mutans, Streptococcus sobrinus, Aggregatibacter actinomycetemcomitans,\u0000Porphyromonas gingivalis, Fusobacterium nucleatum, and Candida albicans to study\u0000their anticariogenic and antiperiodonto pathogenic activities.\u0000\u0000\u0000\u0000these activities were evaluated according to the determination of minimum inhibitory\u0000concentration (MIC) and minimum bactericidal concentration (MBC), anti-biofilm effects, and\u0000the impact on the adhesion to hydroxyapatite, a main component of the tooth.\u0000\u0000\u0000\u0000both leaf and fruit essential oils showed strong antibacterial activity against anaerobic\u0000bacteria: F. nucleatum (MIC of 0.012 %) and P. gingivalis (MIC of 0.025 %). Anticarcinogenic\u0000activity also showed MIC values of 0.06 % against S. mutans and, 0.5 % against S. sobrinus, and\u0000appropriate antifungal activity against C. albicans. It is interesting to note that both oils showed a\u0000significant inhibitory property to biofilm formation by the different studied species and significantly\u0000reduced the adhesion capacity of S. mutans and S. sobrinus to the hydroxyapatite surface at\u0000very low concentrations of 0.12 and 0.5 % for S. mutans and S. sobrinus, respectively.\u0000\u0000\u0000\u0000These results highlight the strong potential of E. globulus essential oils as antimicrobial\u0000and antibiofilm agents, as well as their ability to inhibit bacterial adhesion, which is promising\u0000for the prevention of dental caries and plaque. This implies that E. globulus may be a new\u0000alternative source of substances of medicinal interest that can be used in the pharmaceutical industry\u0000to produce antimicrobial agents against dental caries and oral infectious diseases\u0000","PeriodicalId":10772,"journal":{"name":"Current Bioactive Compounds","volume":"59 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140751574","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-03-19DOI: 10.2174/0115734072294581240311064402
L. Sindhura, Mohd. Nazneen Bobby, K. Srikanth, Helan Soundra Rani Michael, Abu Md Ashif Ikbal, Sabu Thomas, H. Kargarzadeh, Partha Palit
The study addresses the underexplored realm of cytotoxicity evaluation involving binary mixtures of Passiflora caerulea leaf extracts and Titanium nanoparticles (TiO2NPs). The focus lies on understanding the combined effects of these components on cancer cells (A549, U937, and HeLa). In vitro cytotoxicity assessments were employed to evaluate the toxicity of P. caerulea leaf extracts, TiO2NPs, and their combination. The study utilized MTT, NRU, and LDH assays to measure cellular viability. Additionally, reactive oxygen species (ROS) and glutathione levels were assessed alongside the aforementioned assays. The toxicity percentage exhibited dose-dependent behavior for P. caerulea leaf extracts, TiO2NPs, and their combination. Interestingly, when P. caerulea leaf extract and TiO2NPs were combined, the reduction in cell viability was noticeably more than when the exposures were made separately. Moreover, the production of ROS was higher in the combined toxicity scenario, and a more pronounced decrease in glutathione levels was observed compared to individual exposures. The findings suggest that the combined effects of P. caerulea leaf extract and TiO2NPs induce greater cytotoxicity compared to their impacts. This underscores the potential for synergistic cytotoxicity in combined exposure scenarios, warranting further exploration of combined effects in future studies.
该研究涉及西番莲叶提取物和钛纳米粒子（TiO2NPs）二元混合物的细胞毒性评估这一尚未充分开发的领域。研究重点在于了解这些成分对癌症细胞（A549、U937 和 HeLa）的综合影响。体外细胞毒性评估用于评价西番莲叶提取物、TiO2NPs 及其组合的毒性。该研究利用 MTT、NRU 和 LDH 检测法测量细胞活力。对于 P. caerulea 叶提取物、TiO2NPs 和它们的组合，毒性百分比表现出剂量依赖性。有趣的是，当 P. caerulea 叶提取物和 TiO2NPs 混合使用时，细胞活力的降低幅度明显高于单独使用时。此外，与单独接触相比，在联合毒性情况下产生的 ROS 更高，谷胱甘肽水平的下降也更明显。这强调了在联合暴露情况下产生协同细胞毒性的可能性，值得在今后的研究中进一步探讨联合效应。
{"title":"Individual and Dual Cytotoxicity of the Combination of Passiflora Caerulea Leaf Extract and Titanium Oxide Nanoparticles against A549, U937, and\u0000Hela Cells","authors":"L. Sindhura, Mohd. Nazneen Bobby, K. Srikanth, Helan Soundra Rani Michael, Abu Md Ashif Ikbal, Sabu Thomas, H. Kargarzadeh, Partha Palit","doi":"10.2174/0115734072294581240311064402","DOIUrl":"https://doi.org/10.2174/0115734072294581240311064402","url":null,"abstract":"\u0000\u0000The study addresses the underexplored realm of cytotoxicity evaluation\u0000involving binary mixtures of Passiflora caerulea leaf extracts and Titanium nanoparticles\u0000(TiO2NPs). The focus lies on understanding the combined effects of these components on cancer\u0000cells (A549, U937, and HeLa).\u0000\u0000\u0000\u0000In vitro cytotoxicity assessments were employed to evaluate the toxicity of P. caerulea\u0000leaf extracts, TiO2NPs, and their combination. The study utilized MTT, NRU, and LDH assays to\u0000measure cellular viability. Additionally, reactive oxygen species (ROS) and glutathione levels\u0000were assessed alongside the aforementioned assays.\u0000\u0000\u0000\u0000The toxicity percentage exhibited dose-dependent behavior for P. caerulea leaf extracts,\u0000TiO2NPs, and their combination. Interestingly, when P. caerulea leaf extract and TiO2NPs were\u0000combined, the reduction in cell viability was noticeably more than when the exposures were made\u0000separately. Moreover, the production of ROS was higher in the combined toxicity scenario, and a\u0000more pronounced decrease in glutathione levels was observed compared to individual exposures.\u0000\u0000\u0000\u0000The findings suggest that the combined effects of P. caerulea leaf extract and\u0000TiO2NPs induce greater cytotoxicity compared to their impacts. This underscores the potential for\u0000synergistic cytotoxicity in combined exposure scenarios, warranting further exploration of combined\u0000effects in future studies.\u0000","PeriodicalId":10772,"journal":{"name":"Current Bioactive Compounds","volume":"19 S1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140230796","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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