Daphnia pulex is a natural food widely used in aquaculture, and S6K plays an important role in protein translation and ageing. To explore the function of S6K in the ageing process of D. pulex, we cloned and analysed the full-length cDNA of S6K. With the growth of D. pulex, the expression of S6K mRNA first increased, then peaked at 10 days, and then decreased. Western blot analysis showed that the S6K protein expression increased from 1 to 5 days of age, but did not change significantly from 5 to 25 days of age. RNAi knockdown of S6K, reduced the expression of S6K mRNA and S6K protein, and it causes changes in other genes associated with ageing in both sexes. S6K may be involved in reproductive transformation, affecting reproductive capacity, thus significantly affecting the ageing of D. pulex. miRNA with potential regulation of the S6K gene were screened by the bioinformatics method and validated using a dual luciferase reporter gene system. This study deepens our understanding of the mechanisms of ageing in D. pulex and expands our understanding of the role of S6K in the ageing process.
{"title":"Effects of P70 S6 kinase on the ageing process and reproduction of Daphnia pulex (Branchiopoda, Anomopoda)","authors":"Chun-Pu Zhao, Chongyuan Lin, Shun Zhang, Ya-Jun Wang, Shanglian Xu, Dan-Li Wang, Chunyang Guo","doi":"10.1163/15685403-bja10273","DOIUrl":"https://doi.org/10.1163/15685403-bja10273","url":null,"abstract":"\u0000Daphnia pulex is a natural food widely used in aquaculture, and S6K plays an important role in protein translation and ageing. To explore the function of S6K in the ageing process of D. pulex, we cloned and analysed the full-length cDNA of S6K. With the growth of D. pulex, the expression of S6K mRNA first increased, then peaked at 10 days, and then decreased. Western blot analysis showed that the S6K protein expression increased from 1 to 5 days of age, but did not change significantly from 5 to 25 days of age. RNAi knockdown of S6K, reduced the expression of S6K mRNA and S6K protein, and it causes changes in other genes associated with ageing in both sexes. S6K may be involved in reproductive transformation, affecting reproductive capacity, thus significantly affecting the ageing of D. pulex. miRNA with potential regulation of the S6K gene were screened by the bioinformatics method and validated using a dual luciferase reporter gene system. This study deepens our understanding of the mechanisms of ageing in D. pulex and expands our understanding of the role of S6K in the ageing process.","PeriodicalId":10834,"journal":{"name":"Crustaceana","volume":" ","pages":""},"PeriodicalIF":0.6,"publicationDate":"2023-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48170819","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-03-31DOI: 10.1163/15685403-bja10261
E. Williams, L. Bunkley-Williams
{"title":"Further evidence of the symbiotic acorn barnacle, Chelonibia testudinaria (Linnaeus, 1758) (Balanomorpha, Chelonibiidae) on sheepshead, Archosargus probatocephalus (Walbaum, 1792) (Perciformes, Sparidae), and in total 5 families and 2 orders of fishes (formerly superclass Pisces, now gigaclass Actin","authors":"E. Williams, L. Bunkley-Williams","doi":"10.1163/15685403-bja10261","DOIUrl":"https://doi.org/10.1163/15685403-bja10261","url":null,"abstract":"","PeriodicalId":10834,"journal":{"name":"Crustaceana","volume":" ","pages":""},"PeriodicalIF":0.6,"publicationDate":"2023-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44158669","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-03-31DOI: 10.1163/15685403-bja10278
Patricio R. De los Ríos-Escalante, Pedro Jara-Seguel, Á. Contreras, M. Latsague, G. Lara
The commensals on Chilean Decapoda of inland waters are characterized by a marked microendemism. The aim of the present study was to do an exhaustive review on the ectocommensals on Chilean inland-water decapods. The literature revealed the presence of species of Protozoa, viz., eight known only from Parastacus pugnax (Lagenophrys andos, Operculigera insolita, O. parastacis, O. seticola, O. striata, O. taura, Chilenophrys allendei, and Circolagenophrys shiftus), one for both P. pugnax and P. nicoletti (L. anticthos), one for Parastacus pugnax and Samastacus spinifrons (O. asymmetrica), and one reported for Aegla laevis (O. velata). In addition, the presence of flatworms was described, specifically Temnocephala chilensis, associated with A. laevis, P. pugnax, and S. spinifrons, and species of Polychaeta, i.e., Stratiodrilus platensis associated with A. abtao and A. bahamondei, S. aeglaphilus for A. laevis, and S. pugnaxi for P. nicoletti. Biogeographical, ecological and taxonomic implications are discussed.
{"title":"An update and review of commensals on Chilean inland water Decapoda","authors":"Patricio R. De los Ríos-Escalante, Pedro Jara-Seguel, Á. Contreras, M. Latsague, G. Lara","doi":"10.1163/15685403-bja10278","DOIUrl":"https://doi.org/10.1163/15685403-bja10278","url":null,"abstract":"\u0000The commensals on Chilean Decapoda of inland waters are characterized by a marked microendemism. The aim of the present study was to do an exhaustive review on the ectocommensals on Chilean inland-water decapods. The literature revealed the presence of species of Protozoa, viz., eight known only from Parastacus pugnax (Lagenophrys andos, Operculigera insolita, O. parastacis, O. seticola, O. striata, O. taura, Chilenophrys allendei, and Circolagenophrys shiftus), one for both P. pugnax and P. nicoletti (L. anticthos), one for Parastacus pugnax and Samastacus spinifrons (O. asymmetrica), and one reported for Aegla laevis (O. velata). In addition, the presence of flatworms was described, specifically Temnocephala chilensis, associated with A. laevis, P. pugnax, and S. spinifrons, and species of Polychaeta, i.e., Stratiodrilus platensis associated with A. abtao and A. bahamondei, S. aeglaphilus for A. laevis, and S. pugnaxi for P. nicoletti. Biogeographical, ecological and taxonomic implications are discussed.","PeriodicalId":10834,"journal":{"name":"Crustaceana","volume":" ","pages":""},"PeriodicalIF":0.6,"publicationDate":"2023-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44091766","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-03-31DOI: 10.1163/15685403-00004269
{"title":"Tragic Passing of Christoph D. Schubart","authors":"","doi":"10.1163/15685403-00004269","DOIUrl":"https://doi.org/10.1163/15685403-00004269","url":null,"abstract":"","PeriodicalId":10834,"journal":{"name":"Crustaceana","volume":" ","pages":""},"PeriodicalIF":0.6,"publicationDate":"2023-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47592872","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-03-31DOI: 10.1163/15685403-bja10279
S. Gómez
Several individuals of free-living copepods were found in tap water of the Unidad Académica Mazatlán (Instituto de Ciencias del Mar y Limnología). These copepods turned out to belong to a new species of the freshwater genus Paracyclops. Paracyclops poppei, P. hirsutus, and P. chiltoni were the only species reported in Mexican freshwater ecosystems, and the record of P. fiersi sp. nov. is the fourth confirmed species of the genus from Mexico. The new species seems to be related to P. hardingi and P. bromeliacola. The new species differs in the ornamentation of the female antennary coxobasis, and in the posterior, subdistal, medial spinules on the coxa of the first–fourth leg in both sexes. The city of Mazatlan is supplied with groundwater from an aquifer of the Presidio River (south of Mazatlan), and the new species is most probably distributed in the southern state of Sinaloa (north-western Mexico).
{"title":"Paracyclops fiersi sp. nov. (Copepoda, Cyclopoida, Cyclopidae), a new freshwater cyclopoid from north-western Mexico","authors":"S. Gómez","doi":"10.1163/15685403-bja10279","DOIUrl":"https://doi.org/10.1163/15685403-bja10279","url":null,"abstract":"\u0000Several individuals of free-living copepods were found in tap water of the Unidad Académica Mazatlán (Instituto de Ciencias del Mar y Limnología). These copepods turned out to belong to a new species of the freshwater genus Paracyclops. Paracyclops poppei, P. hirsutus, and P. chiltoni were the only species reported in Mexican freshwater ecosystems, and the record of P. fiersi sp. nov. is the fourth confirmed species of the genus from Mexico. The new species seems to be related to P. hardingi and P. bromeliacola. The new species differs in the ornamentation of the female antennary coxobasis, and in the posterior, subdistal, medial spinules on the coxa of the first–fourth leg in both sexes. The city of Mazatlan is supplied with groundwater from an aquifer of the Presidio River (south of Mazatlan), and the new species is most probably distributed in the southern state of Sinaloa (north-western Mexico).","PeriodicalId":10834,"journal":{"name":"Crustaceana","volume":" ","pages":""},"PeriodicalIF":0.6,"publicationDate":"2023-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44500300","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-02-22DOI: 10.1163/15685403-bja10276
Da Pan, Hongying Sun, P. Ng
The identity of the freshwater crab, Aiyunamon tumidum (Wood-Mason, 1871), is clarified after the types are re-examined. The type series is mixed and includes specimens of two species. A syntype from Ponsee in Yunnan, P.R. China, is designated the lectotype of the species; those from Hotha in Yunnan are now referred to A. daiae (Zhang & Sun, in Zhang, Pan, Hao & Sun, 2020). Specimens which have previously been referred to as “A. tumidum” from other parts of Yunnan are here identified as a new species, Aiyunamon fatum sp. nov.. Aiyunamon fatum is most easily distinguished from congeners by the structure of the male first gonopod.
{"title":"The identity of the freshwater crab Aiyunamon tumidum (Wood-Mason, 1871) (Brachyura, Potamidae), with description of a new species from China","authors":"Da Pan, Hongying Sun, P. Ng","doi":"10.1163/15685403-bja10276","DOIUrl":"https://doi.org/10.1163/15685403-bja10276","url":null,"abstract":"\u0000The identity of the freshwater crab, Aiyunamon tumidum (Wood-Mason, 1871), is clarified after the types are re-examined. The type series is mixed and includes specimens of two species. A syntype from Ponsee in Yunnan, P.R. China, is designated the lectotype of the species; those from Hotha in Yunnan are now referred to A. daiae (Zhang & Sun, in Zhang, Pan, Hao & Sun, 2020). Specimens which have previously been referred to as “A. tumidum” from other parts of Yunnan are here identified as a new species, Aiyunamon fatum sp. nov.. Aiyunamon fatum is most easily distinguished from congeners by the structure of the male first gonopod.","PeriodicalId":10834,"journal":{"name":"Crustaceana","volume":" ","pages":""},"PeriodicalIF":0.6,"publicationDate":"2023-02-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45595762","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-02-22DOI: 10.1163/15685403-00004255
P. R. De los Ríos-Escalante
{"title":"Corrigendum regarding the professional affiliation of a co-author","authors":"P. R. De los Ríos-Escalante","doi":"10.1163/15685403-00004255","DOIUrl":"https://doi.org/10.1163/15685403-00004255","url":null,"abstract":"","PeriodicalId":10834,"journal":{"name":"Crustaceana","volume":" ","pages":""},"PeriodicalIF":0.6,"publicationDate":"2023-02-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48785069","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-02-22DOI: 10.1163/15685403-bja10270
P. Clark, C. Froglia
{"title":"The Crustacea taxa established in Volume 35, Bollettino della Società dei Naturalisti in Napoli: Authority date 1923 or 1924?","authors":"P. Clark, C. Froglia","doi":"10.1163/15685403-bja10270","DOIUrl":"https://doi.org/10.1163/15685403-bja10270","url":null,"abstract":"","PeriodicalId":10834,"journal":{"name":"Crustaceana","volume":" ","pages":""},"PeriodicalIF":0.6,"publicationDate":"2023-02-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46957616","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-02-22DOI: 10.1163/15685403-bja10264
P. Ng
A new species of freshwater crab of the genus Isolapotamon Bott, 1968 (Potamidae) is described from western Sabah in Malaysian Borneo. Isolapotamon seowi new species, is the sixth species of Isolapotamon known from Sabah, and differs from its closest congener, I. buntyae Ng, Low & Clark, 2022, from eastern Borneo, in possessing a relatively lower carapace, a more rugose dorsal carapace surface, the postorbital cristae being sharper and subparallel with the frontal margin, relatively shorter fourth ambulatory legs, and the terminal segment of the male first gonopod is gently curved with the subdistal process slightly longer than the distal projection and angled at 90° to the longitudinal axis.
{"title":"A new species of potamid crab, Isolapotamon seowi (Brachyura, Potamoidea) from Kokol Hill in western Sabah, Malaysia, Borneo","authors":"P. Ng","doi":"10.1163/15685403-bja10264","DOIUrl":"https://doi.org/10.1163/15685403-bja10264","url":null,"abstract":"\u0000A new species of freshwater crab of the genus Isolapotamon Bott, 1968 (Potamidae) is described from western Sabah in Malaysian Borneo. Isolapotamon seowi new species, is the sixth species of Isolapotamon known from Sabah, and differs from its closest congener, I. buntyae Ng, Low & Clark, 2022, from eastern Borneo, in possessing a relatively lower carapace, a more rugose dorsal carapace surface, the postorbital cristae being sharper and subparallel with the frontal margin, relatively shorter fourth ambulatory legs, and the terminal segment of the male first gonopod is gently curved with the subdistal process slightly longer than the distal projection and angled at 90° to the longitudinal axis.","PeriodicalId":10834,"journal":{"name":"Crustaceana","volume":" ","pages":""},"PeriodicalIF":0.6,"publicationDate":"2023-02-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46470234","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-02-22DOI: 10.1163/15685403-bja10275
Jing Wang, Jianyong Liu
Ammonia is one of the major environmental pollutants in the aquatic system and poses a great threat to shrimp. In this study, we compared the differences in metabolic enzymes and the mRNA expression of unfolded protein response (UPR)-related genes between the ammonia-resistant (LV-AT) and ammonia-sensitive (LV-AS) strains of Penaeus vannamei, aiming to investigate the different tolerance mechanisms of the two extreme strains underlying ammonia stress. The results showed that elevated ammonia concentrations mainly increased glutamine synthetase (GS) content while inhibiting transglutaminase (TG) content in the muscle of both strains and the contents were significantly higher in LV-AT than in LV-AS after 48 h of ammonia stress (). As a protective or adaptive strategy, the expression of the UPR related genes, including activating transcription factors ATF6, ATF4, and the spliced form of X box binding protein 1 (XBP1) were, respectively, either significantly induced, or inhibited () after ammonia stress. The mRNA expressions of the GRP78 (Bip) gene and the apoptosis gene (caspase 3) are then activated. In hepatopancreas and gills, the associated gene expressions gradually increase in LV-AT, while several related genes in LV-AS were first induced and then gradually inhibited. The expressions of key genes (GRP78, ATF6, XBP1 and ATF4) were significantly higher in LV-AT than that in LV-AS after 48 h of ammonia stress (). These results indicate that some enzymes involved in ammonia metabolism and UPR protection genes tended to be highly expressed in LV-AT. Hence, we speculate that LV-AT has a higher ammonia excretion capacity and a more effective adaptive mechanism after ammonia stress. The experimental results will help to further understand the stress response of P. vannamei to ammonia and provide a new perspective for the underlying molecular mechanism of the ammonia adaptation strategy of at least some strains of P. vannamei.
{"title":"Investigating the differences in metabolism, and the unfolded protein and apoptosis gene responses in two strains of the Pacific white shrimp, Penaeus vannamei Boone, 1931 (Decapoda, Penaeoidea) under ammonia stress","authors":"Jing Wang, Jianyong Liu","doi":"10.1163/15685403-bja10275","DOIUrl":"https://doi.org/10.1163/15685403-bja10275","url":null,"abstract":"\u0000Ammonia is one of the major environmental pollutants in the aquatic system and poses a great threat to shrimp. In this study, we compared the differences in metabolic enzymes and the mRNA expression of unfolded protein response (UPR)-related genes between the ammonia-resistant (LV-AT) and ammonia-sensitive (LV-AS) strains of Penaeus vannamei, aiming to investigate the different tolerance mechanisms of the two extreme strains underlying ammonia stress. The results showed that elevated ammonia concentrations mainly increased glutamine synthetase (GS) content while inhibiting transglutaminase (TG) content in the muscle of both strains and the contents were significantly higher in LV-AT than in LV-AS after 48 h of ammonia stress (). As a protective or adaptive strategy, the expression of the UPR related genes, including activating transcription factors ATF6, ATF4, and the spliced form of X box binding protein 1 (XBP1) were, respectively, either significantly induced, or inhibited () after ammonia stress. The mRNA expressions of the GRP78 (Bip) gene and the apoptosis gene (caspase 3) are then activated. In hepatopancreas and gills, the associated gene expressions gradually increase in LV-AT, while several related genes in LV-AS were first induced and then gradually inhibited. The expressions of key genes (GRP78, ATF6, XBP1 and ATF4) were significantly higher in LV-AT than that in LV-AS after 48 h of ammonia stress (). These results indicate that some enzymes involved in ammonia metabolism and UPR protection genes tended to be highly expressed in LV-AT. Hence, we speculate that LV-AT has a higher ammonia excretion capacity and a more effective adaptive mechanism after ammonia stress. The experimental results will help to further understand the stress response of P. vannamei to ammonia and provide a new perspective for the underlying molecular mechanism of the ammonia adaptation strategy of at least some strains of P. vannamei.","PeriodicalId":10834,"journal":{"name":"Crustaceana","volume":" ","pages":""},"PeriodicalIF":0.6,"publicationDate":"2023-02-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43541806","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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