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High throughput in vitro production of somatic embryoswith innovative cryo cell banking system 利用创新的冷冻细胞库系统高通量体外生产体细胞胚胎
Pub Date : 2022-09-14 DOI: 10.15406/hij.2022.06.00259
Weiming Wang, Lambert Sanders, Alice Perry, Ebonee Duncan
ArborGen Inc. was originally created as a partnership for forestry research in 2000 by West Rock (MWV), International Paper and Rubicon (Fletcher Challenge). It now produces the largest number of both conventional and advanced genetic tree seedlings in the world. Among its advanced genetics offerings, varietals have the highest level of genetic traits in yield and timber quality such as height, diameter growth, disease resistance and etc. To scale up varietals, in vitro process named somatic embryogenesis (SE) is used as a mean to mass propagate SE seedlings. Over the years of R&D efforts, we recognized that a key challenge for scale-up is the lack of a stable and predictable bioprocess platform. The primary causes that impact stability and predictability include a challenge to receive consistent supplies of good starting cell materials, the risk of somaclonal variation due to long term exposure tomaintenance media containing hormones and risk of contamination or loss from environmental fluctuations. Accordingly our SE R&D program has been targeted to develop a robust bioprocess platform to reduce these variabilities. One of our major achievement is the development of a cell banking (CB) system, which provides high quality starting cell materials for SE production. The cell banking system includes the creations of research cell banks (RCB) and master cell banks (MCB). After the cell banks are created, the banked cells are tested and validated for their qualification for embryo production. Currently, we successfully integrated the cell banking technology into somatic embryo production in a number of economically important tree species and crops, such as pine, spruce, sweetgum, sugarcane, avocado and banana. With the SE&CB platform, the somatic embryo & somatic seedling yields are consistent, predictable and are produced cost-efficiently on a commercial scale.
ArborGen公司最初是由West Rock (MWV)、International Paper和Rubicon (Fletcher Challenge)在2000年建立的林业研究合作伙伴关系。它现在生产世界上最多的传统和先进的遗传树苗。在其高级遗传产品中,品种在产量和木材品质方面具有最高水平的遗传性状,如高度,直径生长,抗病性等。为了扩大品种规模,采用体外体细胞胚胎发生(somatic embryogenesis, SE)作为大量繁殖体细胞胚胎的手段。经过多年的研发工作,我们认识到扩大规模的一个关键挑战是缺乏稳定和可预测的生物工艺平台。影响稳定性和可预见性的主要原因包括:难以持续供应良好的起始细胞材料;由于长期接触含有激素的维持介质而存在体细胞无性系变异的风险;以及环境波动造成污染或损失的风险。因此,我们的SE研发计划旨在开发一个强大的生物工艺平台来减少这些变化。我们的主要成就之一是细胞库(CB)系统的开发,该系统为SE生产提供了高质量的起始细胞材料。细胞库系统包括研究细胞库(RCB)和主细胞库(MCB)的创建。细胞库创建后,对储存的细胞进行测试和验证,以确定其胚胎生产的资格。目前,我们已经成功地将细胞库技术整合到一些经济上重要的树种和作物的体细胞胚胎生产中,如松树、云杉、甜桉树、甘蔗、鳄梨和香蕉。通过SE&CB平台,体细胞胚和体细胞苗的产量是一致的,可预测的,并且在商业规模上具有成本效益。
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引用次数: 0
Role of Nalanda college of agriculture in promoting agricultural education in Tamil Nadu 那烂陀农业学院在促进泰米尔纳德邦农业教育中的作用
Pub Date : 2022-08-31 DOI: 10.15406/hij.2022.06.00258
S. C, S. R, Sheebha G, S. M, Mohanraj M
The Study focuses on major areas in which agricultural education is imparted by incorporating major and minor courses which are related to agriculture and allied subjects which are helping in the promotion of career orientation. Another aspect of the study is Promoting greenery to the environment by the students of agriculture and earns hands on training in the areas like bee keeping, mushroom cultivation, Ornamental Nursery, Entrepreneurial Development, Commercial Seed Production, Dry land farming, sericulture, vermicompost production, azolla production which are giving way to the students in practicing of agribusiness and its initiatives in their region of residence. In this respect, this article addresses how an educational institution could train the students in making use of the available dry lands for effective crop production front by employing the agricultural labour force in making the dry lands into a big greenery. This might be an example to the dry land farmers to cope with and elevate their lifestyle in an appreciable way and it will also promote the farming initiatives with courage and confidence wherever the land and irrigation facility are available. Certain students are also taking the lands on lease to promote agriculture in a big way. This paper is a paradigm for the persons who are taking initiatives in agriculture to make farming as their career path.
这项研究的重点是传授农业教育的主要领域,纳入了与农业和相关科目有关的主要和次要课程,这些课程有助于促进职业方向。该研究的另一个方面是通过农业学生促进环境绿化,并在养蜂,蘑菇种植,观赏苗圃,企业发展,商业种子生产,旱地耕作,蚕桑养殖,蚯蚓堆肥生产,杜鹃花生产等领域获得培训,这些领域正在让位于学生在其居住地区的农业综合企业实践及其倡议。在这方面,本文讨论了教育机构如何通过雇用农业劳动力使旱地成为一个大的绿色植物来培训学生利用可用的旱地进行有效的作物生产。这可能是旱地农民以一种可观的方式应对和提高他们生活方式的一个例子,它也将以勇气和信心促进农业倡议,无论在哪里,土地和灌溉设施都是可用的。一些学生还租用土地,以大规模促进农业发展。本文为积极从事农业的人以农业为职业发展道路提供了一个范例。
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引用次数: 0
http://medcraveonline.com/HIJ/volume_issues?issueId=3676&volumeId=881 http://medcraveonline.com/HIJ/volume_issues?issueId=3676&volumeId=881
Pub Date : 2022-08-23 DOI: 10.15406/hij.2022.06.00257
T. Mbi, Tatah Eugene Lemzeyuy, Ambebe Titus, Sirri Josepha
Direct seeding of carrot by broadcasting usually leads to overcrowding of seedlings after emergence. To reduce the undesirable effects of high crop density, uniform seedling distribution is ensured by thinning and discarding of a large number of seedlings. Given the expensive nature of carrot seeds, transplanting of thinned seedlings could be a more useful option than discarding them. It was within this context that this study was carried out at the teaching and research farm of the college of technology of the University of Bamenda, Cameroon to determine the effect of transplanting on root yield of imperator carrot variety. The experimental layout was a randomized complete block design (RCBD) with four treatments being T0=direct seeding which served as the absolute Control, and three transplanting days after emergence (DAE) notably T1=Transplanting at 5 DAE, T2=Transplanting 10DAE, T3=Transplanting 15DAE. Variables evaluated were growth in tap root length over time, length of root, fresh weight roots, rate of root forking /plant and root yield (t/ha). Results show that trial under T0 presented significantly higher number of straight roots carrot while all transplanted carrots regardless of the day of transplanting developed 1 to 3 forks at harvest (p<0.05). Highly significant differences at P<0.05 were noticed at the level of root yield, with direct seeding trial (T0) recording a significantly higher yield of4.25t/ha against1.81, 2.4, and 1.73 t/ha for transplanting at 5 DAE, 10DAE and 15DAE.
通过播种法直接播种胡萝卜通常会导致出苗后秧苗过度拥挤。为了减少作物密度高的不良影响,通过疏苗和大量弃苗来保证幼苗分布均匀。考虑到胡萝卜种子的昂贵性质,移植细苗可能比丢弃它们更有用。正是在这样的背景下,本研究在喀麦隆巴门达大学技术学院的教研农场开展,以确定移栽对帝王胡萝卜品种根产量的影响。试验布局采用随机完全区组设计(RCBD), 4个处理分别为T0=直接播种作为绝对对照,出苗后3天(DAE), T1= 5天移栽,T2= 10天移栽,T3= 15天移栽。评估的变量是随时间生长的主根长度、根长、根鲜重、根分叉率/株和根产量(t/ha)。结果表明:T0处理下直根胡萝卜数量显著增加,且不论移栽天数,所有移栽胡萝卜收获时均长出1 ~ 3根叉(p<0.05)。在根产量水平上差异极显著(P<0.05),直接播种试验(T0)的产量为4.25t/ha,显著高于移栽在5、10和15DAE时的1.81、2.4和1.73 t/ha。
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引用次数: 0
Pathogenesis of begomoviruses in Tomato 番茄begomovirus的发病机制
Pub Date : 2022-08-10 DOI: 10.15406/hij.2022.06.00256
Londoño A, B. B
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引用次数: 0
Research trend analysis of Leucojum aestivum based on Citespace visualization software 基于Citespace可视化软件的白菖蒲研究趋势分析
Pub Date : 2022-07-29 DOI: 10.15406/hij.2022.06.00255
Wen Wang, Huan Tang, Junce Zhou, Taoze Sun, Hongna Mu
The secondary metabolite of Leucojum aestivum lycorine, is of high medicinal value and has attracted much attention internationally. Therefore, it is essential to review and analyze the existing literature on L. aestivum to clarify the research progress, research bottlenecks, and applications of lycorine in landscapes. In this study, we used the Web of Science (WOS) and CNKI Chinese journal database as data sources to analyze the bibliometric analysis of L. aestivum-related literature in terms of the number of publications, journal sources, research institutions, keywords, and co-cited literature using Citespace visualization software. The results showed that the number of L. aestivum-related studies decreased compared to previous years, but the number of publications showed a high growth trend in this research frontier. Bulgaria has a significant dominance in L. aestivum-related research, while China has fewer publications in this field but focuses more on the frontiers of the discipline. The frontiers of L. aestivum research concentrate on improving in vitro culture devices, changing plant culture strategies, and adding chemical additives to improve the yield of compounds in plants; new methods for lycorine synthesis; identification of ornamental plant species and detection of compounds by electrochemical methods; practical applications of lycorine in medicine and its mechanism of action. It is inferred that future research will focus on the ornamental value, medicinal value, cultivation methods, and innovation of lycorine synthesis methods of aestivum.
白菖蒲石蒜碱是白菖蒲的次生代谢产物,具有很高的药用价值,在国际上受到广泛关注。因此,有必要对现有的石蒜碱研究文献进行梳理和分析,以明确石蒜碱的研究进展、研究瓶颈以及在景观中的应用。本研究以Web of Science (WOS)和中国知网中文期刊数据库为数据源,利用Citespace可视化软件,从出版物数量、期刊来源、研究机构、关键词、共被引文献等方面对我国枸杞相关文献进行文献计量分析。结果表明,与往年相比,与羊乳杆菌相关的研究数量有所减少,但在这一研究前沿领域发表的论文数量呈高增长趋势。保加利亚在L. aestivum相关研究方面具有显著的优势,而中国在该领域的出版物较少,但更多地关注该学科的前沿。研究的前沿主要集中在改进离体培养装置、改变植物培养策略、添加化学添加剂提高植物体内化合物产量等方面;石蒜碱合成的新方法电化学方法鉴定观赏植物种类及化合物石蒜碱在医学上的实际应用及其作用机制。推测今后的研究将集中在石蒜的观赏价值、药用价值、栽培方法以及石蒜碱合成方法的创新等方面。
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引用次数: 0
Applicational study on the edible university landscape under COVID-19 epidemic circumstance 新型冠状病毒疫情下高校食用景观的应用研究
Pub Date : 2022-07-25 DOI: 10.15406/hij.2022.06.00254
Junce Zhou, Taoze Sun, Xiao‐rui Guo, Wen Wang, Hongna Mu
All of universities or colleges have to solve the big challenges from the outbreak of COVID-19. Quarantining of Campus has been being the regular management policy, the activities of all students were restricted inside the campus. Under such status, campus landscape quality. In this study, the concept of Edible Landscape has been introduced into the campus planning and designing. The construction principles of healing, practicality, ecology, economy and adaptation to local conditions were illustrated, in the light of COVID-19 controlling and prevention. Furthermore, a construction strategy of edible landscape in assisting related teaching and researching activities, taking away negative emotions, and producing fresh and delicious fruits and vegetables, which can offset the shortcoming of traditional landscape of ornamental-value-centered. The construction principles and strategies of this paper put forward novel thinking and references to carry out the construction and development of campus landscape with ecology, healthy and effective traits, to the application of edible and pluralistic school landscape.
所有高校都必须解决新冠肺炎疫情带来的巨大挑战。隔离校园一直是学校的常规管理政策,所有学生的活动都被限制在校园内。在这样的现状下,校园景观质量。本研究将食用景观的概念引入校园规划设计中。结合新冠肺炎疫情防控,阐述了愈合性、实用性、生态性、经济性、因地制宜的建设原则。在辅助相关教研活动、消弭负面情绪、生产新鲜美味的果蔬等方面提出食用景观的建设策略,可以弥补传统景观以观赏价值为中心的不足。本文的建设原则和策略为开展具有生态、健康、有效特色的校园景观建设和发展,对可食用、多元化的校园景观的应用提出了新颖的思考和借鉴。
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引用次数: 0
Rooftop gardening in the globe: advantages and challenges 全球屋顶园艺:优势与挑战
Pub Date : 2022-07-22 DOI: 10.15406/hij.2022.06.00253
A. Quddus
Due to rapid increase of global urbanization food demand is rising for the urban inhabitants, especially, massive consumption demand for green vegetables and fruits. Rooftop gardening is a valuable mode of getting these fresh and nutrition rich food for urban dwellers. Also, roof farming is an alternative means of land use and in the countries with less agricultural lands. This farming system serves the healthy foods and progress in food security of the urban people. It improves urban atmosphere through reducing pollution, enhance greening environment and provides households’ recreation. Thus, a review study has done to know the scenario of rooftop farming in the urban community along with advantages and challenges. Previous research denounced that insight and consciousness of roof gardening has enriched and affords wellbeing, social value and enjoyable farming to the building residents. Knowledge and awareness of gardening should be disseminated, commercial roof farming should be popularized and the challenges regarding urban rooftop gardening should try to overcome.
由于全球城市化的快速发展,城市居民对食品的需求不断上升,特别是对绿色蔬菜和水果的消费需求巨大。屋顶园艺是城市居民获得这些新鲜和营养丰富的食物的一种有价值的模式。此外,屋顶农业是土地利用的另一种方式,在农业用地较少的国家也是如此。这种农业系统为城市居民的健康食品和粮食安全的进步服务。它通过减少污染,改善城市氛围,增强绿化环境,为家庭提供娱乐。因此,一项综述研究已经完成,以了解屋顶农业在城市社区的情况以及优势和挑战。之前的研究谴责了屋顶园艺的洞察力和意识,为建筑居民提供了丰富和幸福、社会价值和愉快的耕作。应该传播园艺知识和意识,推广商业化屋顶种植,努力克服城市屋顶园艺面临的挑战。
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引用次数: 0
Ralstonia solanacearum: a potential target by organic ecology in ginger (Zingiber officinale Roscoe) production 生姜有机生态学研究的潜在目标
Pub Date : 2022-07-13 DOI: 10.15406/hij.2022.06.00252
Yu Gan, Jingjie Ban, Rongfang Guo, Yuling Lin, Z. Lai, X. XuHan
Extensive cultivation of ginger for years caused ecological environment worse off and ginger productivity decrease due to wilt diseases, which also happens in nearly all crops in continuous cropping, especially in Ralstonia solanacearum caused wilt farmlands. In the present work, based on analysis of Ralstonia solanacearum species complex (RSSC) host plant phylogeny and RSSC control factors, an organic ecological method to combat the wilt is proposed that is expected to guarantee continuous cropping of ginger (Zingiber officinale Rosc.) achieving double harvest in economy and environment.
姜的长年粗放型栽培,导致生态环境恶化,姜产量下降,几乎所有连作作物都会发生黄萎病,尤其是青枯病引起的农田。本文在分析茄青枯菌(Ralstonia solanacearum)寄主植物系统发育及其控制因素的基础上,提出了一种有机生态防治方法,有望保证生姜连作在经济和环境上实现双丰收。
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引用次数: 0
Thermo regulation of honeybee (apismellifera l.) Hives under extreme temperatures 蜜蜂(apismellifera)的体温调节极端温度下的荨麻疹
Pub Date : 2022-07-12 DOI: 10.15406/hij.2022.06.00251
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引用次数: 0
Quality indicators in nanche (Byrsonima crassifolia L.) fruits from Guerrero and Chiapas, Mexico 墨西哥格雷罗州和恰帕斯州南切(Byrsonima crassifolia L.)水果的质量指标
Pub Date : 2022-06-28 DOI: 10.15406/hij.2022.06.00250
Jaimes-García Me, González-Zavaleta Sv, Noriega-Cantú Dh, Pereyda-Hernández J, G. S., González-Mateos R
The nanche (Byrsonima crassifolia) is a wild, rustic and native plant of Mexico and Central America, it adapts to different edaphic and climatic conditions. The objective was to analyze the quality indicators of the nanche fruits of Guerrero and Chiapas. Completely random samplings were carried out in three and two localities of Guerrero and Chiapas, respectively, in 5 trees of each locality, collecting 50fruits per tree. The indicators analyzed were: fruit weight, fruit density, polar and equatorial diameter, oBrix, firmness, epidermis thickness, pulp weight, seed weight, luminosity, chromaticity and hue. The correlation between equatorial diameter/weight and lightness/chroma with r 2 of 0.93. Localities of Guerrero with greater statistical difference: Paso del Real was in weight, equatorial diameter; epidermis and seed. The fruits from Las Tunas were outstanding in oBrix, hue and seed weight, and those from San Jerónimo, Guerrero, recorded higher fruit density and equatorial diameter. The localities of Chiapas with the greatest statistical differences: Santo Domingo in density, polar diameter, firmness and pulp, and Mixiquito in fruit density and luminosity. It is concluded that the quality indicators of the nanche fruits collected in Guerrero and Chiapas have a wide distribution and decision making in the selection of trees with specific characteristics.
nanche (Byrsonima crassifolia)是墨西哥和中美洲的一种野生,乡村和本地植物,它适应不同的地理和气候条件。目的是分析格雷罗州和恰帕斯州南切水果的质量指标。完全随机抽样分别在格雷罗州和恰帕斯州的3个和2个地区进行,每个地区5棵树,每棵树采集50个果实。分析的指标有:果实重、果实密度、极径和赤道径、oBrix、硬度、表皮厚度、果肉重、种子重、光度、色度和色调。赤道径/重与轻度/色度的相关系数r2为0.93。格雷罗州的地方统计差异较大:帕索德尔雷亚尔的重量,赤道直径;表皮和种子。来自Las Tunas的果实在颜色、色相和种子重量方面表现突出,而来自San Jerónimo和Guerrero的果实密度和赤道直径较高。统计差异最大的恰帕斯地区:密度、极径、硬度和果肉在圣多明各,果实密度和亮度在密西基多。结果表明,格雷罗州和恰帕斯州南柑果实的品质指标分布广泛,在选择具有特定特征的树种时具有决策作用。
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引用次数: 0
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Horticulture International Journal
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