Pub Date : 2023-11-08DOI: 10.2174/0115734129270020231102081109
Parikh Nisha, Parmar Srushti, Dave Bhavarth, Mohammad Kaif, Parikh Palak
Abstract: Cervical cancer is one of the most prevalent forms of cancer occurring across the world and it has been observed that about 99.7% of cervical cancer cases occur due to infections with the Human papillomavirus (HPV). Over prolonged durations, cervical cancer can lead to complications such as vaginal bleeding, itching, and in more severe instances, even the fatality of the individual. Cervical cancer is an essential cause of death at an early age as it affects young women higher than other populations. The most frequent drugs used in its treatment include antiangiogenic drugs. This review summarizes analytical techniques used for the quantification of anti-angiogenic agentsBevacizumab, Sunitinib, Pazopanib, Brivanib, and Imatinib. Furthermore, an in-depth description of numerous techniques including NIR (1), HPLC (10), LC-MS (28), and HPTLC (1) approaches used to determine and quantify these agents have been provided in this review. Based on the matrix utilized, the following details were discussed: analytical conditions, detection limits, and solvent used in sample preparation. Our review holds significant importance within the scientific community, offering valuable insights into commonly employed measurement techniques and the latest advancements in these approaches.
{"title":"Comprehensive Review on Analytical and Bioanalytical Methods for Quantification of Anti-Angiogenetic Agents used in Treatment of Cervical Cancer","authors":"Parikh Nisha, Parmar Srushti, Dave Bhavarth, Mohammad Kaif, Parikh Palak","doi":"10.2174/0115734129270020231102081109","DOIUrl":"https://doi.org/10.2174/0115734129270020231102081109","url":null,"abstract":"Abstract: Cervical cancer is one of the most prevalent forms of cancer occurring across the world and it has been observed that about 99.7% of cervical cancer cases occur due to infections with the Human papillomavirus (HPV). Over prolonged durations, cervical cancer can lead to complications such as vaginal bleeding, itching, and in more severe instances, even the fatality of the individual. Cervical cancer is an essential cause of death at an early age as it affects young women higher than other populations. The most frequent drugs used in its treatment include antiangiogenic drugs. This review summarizes analytical techniques used for the quantification of anti-angiogenic agentsBevacizumab, Sunitinib, Pazopanib, Brivanib, and Imatinib. Furthermore, an in-depth description of numerous techniques including NIR (1), HPLC (10), LC-MS (28), and HPTLC (1) approaches used to determine and quantify these agents have been provided in this review. Based on the matrix utilized, the following details were discussed: analytical conditions, detection limits, and solvent used in sample preparation. Our review holds significant importance within the scientific community, offering valuable insights into commonly employed measurement techniques and the latest advancements in these approaches.","PeriodicalId":10889,"journal":{"name":"Current Pharmaceutical Analysis","volume":"18 6","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-11-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135430319","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-10-25DOI: 10.2174/0115734129262343231020105935
Guilherme Orsay, Khrissy Medeiros, Elcio Oliveira
Abstract is not required,
摘要不是必需的，
{"title":"Use of Uncertainty Information in Conformity Assessment in the Pharmaceutical Industry","authors":"Guilherme Orsay, Khrissy Medeiros, Elcio Oliveira","doi":"10.2174/0115734129262343231020105935","DOIUrl":"https://doi.org/10.2174/0115734129262343231020105935","url":null,"abstract":"Abstract is not required,","PeriodicalId":10889,"journal":{"name":"Current Pharmaceutical Analysis","volume":"30 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135219137","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-10-20DOI: 10.2174/0115734129253094231018115646
Jiaye Tian, Ran Bai, Ziyue Chen, Piaoran Qin, Xingchao Liu, Haoran Shen, Li Zhou, Qiuhong Guo
Background:: Bellidifolin (BEL) has a decent enemy of myocardial fibrosis impact, and its preparation into nano-micelles can build security and great biocompatibility in vitro and in vivo. The pharmacokinetic assessment of BEL can be utilized as the reason for the security and viability of BEL in clinical use. Objective:: This research aimed to establish an effective UPLC-MS/MS strategy for assuring BEL in rodent plasma and concentrating on its pharmacokinetics in vivo. objective: This research aimed to establish an effective UPLC-MS/MS strategy for assuring BEL in rodent plasma and concentrating on its pharmacokinetics in vivo. Methods:: Luteolin was utilized as an internal standard (IS). Chromatographic separation was accomplished utilizing a UPLC HSS T3 column (2.1 ×100 mm, 1.8 μm) section using a mobile phase of 0.1% acetonitrile (A) and 0.1% formic acid in water (B) with gradient elution. Electrospray ionization (ESI) coupled mass spectrometry was applied in various response checking (MRM) modes with negative ionization. Results:: The pharmacokinetic behaviour of bellidifolin nano-micelles in vivo showed that the peak concentration (Cmax) was 1666.19±479.92 μg/L, the time to peak (Tmax) was 0.167 h, and the apparent elimination half-life (t1/2) was 7.60±3.58 h. The plasma clearance rate (CL/F) was 1.15±0.48 L/h/kg, the apparent volume of distribution (V/F) was 14.38±11.04, the area under the curve (AUC) was 8292.57±4193.13 μg/L*h, and the mean retention time (MRT) was 9.70±4.55 h. Conclusion:: The method was successfully applied to the plasma pharmacokinetics of bellidifolin nano-micelles after intragastric administration to rats. other: None.
{"title":"A Rapid and Sensitive UPLC-MS/MS Method for the Determination of Bellidifolin and Pharmacokinetics Study of Bellidifolin Nano-microcells","authors":"Jiaye Tian, Ran Bai, Ziyue Chen, Piaoran Qin, Xingchao Liu, Haoran Shen, Li Zhou, Qiuhong Guo","doi":"10.2174/0115734129253094231018115646","DOIUrl":"https://doi.org/10.2174/0115734129253094231018115646","url":null,"abstract":"Background:: Bellidifolin (BEL) has a decent enemy of myocardial fibrosis impact, and its preparation into nano-micelles can build security and great biocompatibility in vitro and in vivo. The pharmacokinetic assessment of BEL can be utilized as the reason for the security and viability of BEL in clinical use. Objective:: This research aimed to establish an effective UPLC-MS/MS strategy for assuring BEL in rodent plasma and concentrating on its pharmacokinetics in vivo. objective: This research aimed to establish an effective UPLC-MS/MS strategy for assuring BEL in rodent plasma and concentrating on its pharmacokinetics in vivo. Methods:: Luteolin was utilized as an internal standard (IS). Chromatographic separation was accomplished utilizing a UPLC HSS T3 column (2.1 ×100 mm, 1.8 μm) section using a mobile phase of 0.1% acetonitrile (A) and 0.1% formic acid in water (B) with gradient elution. Electrospray ionization (ESI) coupled mass spectrometry was applied in various response checking (MRM) modes with negative ionization. Results:: The pharmacokinetic behaviour of bellidifolin nano-micelles in vivo showed that the peak concentration (Cmax) was 1666.19±479.92 μg/L, the time to peak (Tmax) was 0.167 h, and the apparent elimination half-life (t1/2) was 7.60±3.58 h. The plasma clearance rate (CL/F) was 1.15±0.48 L/h/kg, the apparent volume of distribution (V/F) was 14.38±11.04, the area under the curve (AUC) was 8292.57±4193.13 μg/L*h, and the mean retention time (MRT) was 9.70±4.55 h. Conclusion:: The method was successfully applied to the plasma pharmacokinetics of bellidifolin nano-micelles after intragastric administration to rats. other: None.","PeriodicalId":10889,"journal":{"name":"Current Pharmaceutical Analysis","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135666196","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
background: Zhachong shisan pills (ZSP) is a traditional Mongolian medicine, included in the drug standard of the Ministry of the health of the people's Republic of China (Mongolian medicine volume) It is a watered pill preparation composed of 13 material medicas, including Aconiti Kusnezoffii Radix Cocta, Terminalia chebula Retz, Acori Tatarinowii Rhizoma, Aucklandiae Radix, Caryophylli Flos, Glycyrrhizae Radix et Rhizoma, Moschus, Aquilariae Lignum Resinatum, Myristicae Semen, Margarita (made), Magnetitum (calcined), Coral (made) and Red Claystone, which has the functions of dispelling wind and dredging orifices, relaxing muscles and activating blood circulation, calming the nerves. objective: The purpose of this study is to establish Chromatographic fingerprint of Zhachong Shisan pills (ZSP), and to explore its active components and mechanism of analgesic and anti-inflammatory action. method: First, the Personal Compound Database and Library (PCDL) of ZSP was constructed through literature mining, and then the chemical composition and fingerprint of methanol extractions from 8 batches of ZSP were studied using High-Performance Liquid Chromatography with Quadrupole Time-Of-Flight tandem Mass Spectrometry (HPLC-QTOF-MS) technology. Furthermore, network pharmacology was used to explore the active compounds, potential targets, and signal pathways of analgesic and anti-inflammatory activity of ZSP. result: A total of 102 compounds were detected in positive and negative ion mode. Fifty-six characteristic peaks in positive ion mode and 78 characteristic peaks in negative ion mode were confirmed as common peaks in the fingerprint of ZSP. Through network pharmacology research, the effective components, key targets, and signal pathways of ZSP for the treatment of cerebral apoplexy by analgesic and anti-inflammatory effects were all analyzed. conclusion: This study explained the substance basis of the analgesic and anti-inflammatory effects of Zhachong Shisan Pills, and explores its possible mechanisms, providing ideas for rational clinical medication and in-depth pharmacological research.
{"title":"Explore the Constituents and Mechanism of Traditional Chinese Medicine Preparation Zhachong Shisan Pills Based on HPLC-QTOF-MS and Network Pharmacology","authors":"Yueqiang Xin, Yuli Sang, Yanjun Hao, Jing Lu, Xiang Ji, Manman Tang, Lijiang Chen","doi":"10.2174/0115734129259758230924070432","DOIUrl":"https://doi.org/10.2174/0115734129259758230924070432","url":null,"abstract":"background: Zhachong shisan pills (ZSP) is a traditional Mongolian medicine, included in the drug standard of the Ministry of the health of the people's Republic of China (Mongolian medicine volume) It is a watered pill preparation composed of 13 material medicas, including Aconiti Kusnezoffii Radix Cocta, Terminalia chebula Retz, Acori Tatarinowii Rhizoma, Aucklandiae Radix, Caryophylli Flos, Glycyrrhizae Radix et Rhizoma, Moschus, Aquilariae Lignum Resinatum, Myristicae Semen, Margarita (made), Magnetitum (calcined), Coral (made) and Red Claystone, which has the functions of dispelling wind and dredging orifices, relaxing muscles and activating blood circulation, calming the nerves. objective: The purpose of this study is to establish Chromatographic fingerprint of Zhachong Shisan pills (ZSP), and to explore its active components and mechanism of analgesic and anti-inflammatory action. method: First, the Personal Compound Database and Library (PCDL) of ZSP was constructed through literature mining, and then the chemical composition and fingerprint of methanol extractions from 8 batches of ZSP were studied using High-Performance Liquid Chromatography with Quadrupole Time-Of-Flight tandem Mass Spectrometry (HPLC-QTOF-MS) technology. Furthermore, network pharmacology was used to explore the active compounds, potential targets, and signal pathways of analgesic and anti-inflammatory activity of ZSP. result: A total of 102 compounds were detected in positive and negative ion mode. Fifty-six characteristic peaks in positive ion mode and 78 characteristic peaks in negative ion mode were confirmed as common peaks in the fingerprint of ZSP. Through network pharmacology research, the effective components, key targets, and signal pathways of ZSP for the treatment of cerebral apoplexy by analgesic and anti-inflammatory effects were all analyzed. conclusion: This study explained the substance basis of the analgesic and anti-inflammatory effects of Zhachong Shisan Pills, and explores its possible mechanisms, providing ideas for rational clinical medication and in-depth pharmacological research.","PeriodicalId":10889,"journal":{"name":"Current Pharmaceutical Analysis","volume":"8 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135884766","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-10-04DOI: 10.2174/0115734129250659230929105800
Jiajia Zou, Lin Yang, Xiaoli Xu, Yan Li, Dan He
Background:: N-nitrosodimethylamine (NDMA) are a sort of genotoxic impurities (GTIs) having strong carcinogenic effects and obvious hepatotoxicity. To monitor the NDMA content of metformin hydrochloride sustained-release tablets and enteric capsules in China from 2018 to 2022, a GC-MS/MS method was established and validated. background: N-nitrosodimethylamine (NDMA) is a sort of genotoxic impurities (GTIs) which has strong carcinogenic effect and obvious hepatotoxicity. To monitor NDMA content of in Metformin Hydrochloride Sustained-release Tablets and Enteric Capsules in China from 2018 to 2022, a GC-MS/MS method was established and verified. Methods:: The chromatographic column was Agilent VF-WAXms capillary column (30 m×0.25 mm, 0.25 μm). The GC-MS/MS method was equipped with multiple reaction monitoring (MRM) modes. To assess the quantity of NDMA, the molecular ion at mass-to-charge (m/z) of 74-44 was monitored under the 6 V collision energy, and to assess the quality of NDMA monitoring, the molecular ions at m/z 74-42 were determined. A total of 143 batches of metformin hydrochloride-finished products from 35 enterprises were determined by this method. Results:: The linear range of the method was 0.25 ~ 50.00 ng/mL, r = 0.9998, S/N>10, and the limit of detection and quantitation were 0.06 ng/mL and 0.21 ng/mL, respectively. The average recovery was 98.62%, and the RSD was 4.31%. All batches of enteric capsules met the requirements; 38.21% of the 123 batches sustained-release tablets still exceeded the acceptable daily intake. Conclusion:: The presented method is sensitive, accurate, precise, and available for both enteric capsules and sustained-release tablets of metformin hydrochloride, which can provide a reference for their quality control. The over-limit phenomenon of NDMA in metformin hydrochloride products poses new challenges and requirements for both the State Drug Administration and enterprises. result: The linear range of the method was 0.25 ~ 50.00 ng/mL, r = 0.9998, S/N>10, and the limit of detection was 0.21 ng/mL. The average recovery was 98.62 %, and the RSD was 4.31%. All batches of enteric capsules met the requirements, 38.21% of the 123 batches sustained-release tablets still exceeded the acceptable daily intake. other: nothing
背景:n -亚硝基二甲胺(NDMA)是一类具有强致癌性和明显肝毒性的遗传毒性杂质(GTIs)。为监测2018 - 2022年国内盐酸二甲双胍缓释片和肠溶胶囊中NDMA的含量，建立并验证了GC-MS/MS法。背景:n -亚硝基二甲胺(NDMA)是一种具有强致癌性和明显肝毒性的遗传毒性杂质(GTIs)。为监测2018 - 2022年国内盐酸二甲双胍缓释片和肠溶胶囊中NDMA的含量，建立并验证了GC-MS/MS法。方法:色谱柱为Agilent VF-WAXms毛细管柱(30 m×0.25 mm, 0.25 μm)。GC-MS/MS方法具有多种反应监测(MRM)模式。为了评估NDMA的数量，在6 V碰撞能量下监测74-44的质量电荷比(m/z)的分子离子，测定74-42的m/z的分子离子，以评估NDMA的监测质量。采用该方法对35家企业生产的143批盐酸二甲双胍成品进行了检测。结果:方法线性范围为0.25 ~ 50.00 ng/mL, r = 0.9998, S/N>10，检出限和定量限分别为0.06 ng/mL和0.21 ng/mL。平均加样回收率为98.62%，RSD为4.31%。所有批次肠溶胶囊均符合要求;123批缓释片中仍有38.21%超过可接受日摄入量。结论:本方法灵敏、准确、精密度高，适用于盐酸二甲双胍肠溶胶囊和缓释片，可为其质量控制提供参考。盐酸二甲双胍产品中NDMA的超标现象对国家药品监督管理局和企业提出了新的挑战和要求。结果:方法的线性范围为0.25 ~ 50.00 ng/mL, r = 0.9998, S/N>10，检出限为0.21 ng/mL。平均加样回收率为98.62%，RSD为4.31%。所有批次肠溶胶囊均符合要求，123批次缓释片中仍有38.21%超过日可接受摄入量。其他:无
{"title":"Method Validation and Monitoring of N-nitrosodimethylamine in Metformin Hydrochloride Products in China by GC-MS/MS","authors":"Jiajia Zou, Lin Yang, Xiaoli Xu, Yan Li, Dan He","doi":"10.2174/0115734129250659230929105800","DOIUrl":"https://doi.org/10.2174/0115734129250659230929105800","url":null,"abstract":"Background:: N-nitrosodimethylamine (NDMA) are a sort of genotoxic impurities (GTIs) having strong carcinogenic effects and obvious hepatotoxicity. To monitor the NDMA content of metformin hydrochloride sustained-release tablets and enteric capsules in China from 2018 to 2022, a GC-MS/MS method was established and validated. background: N-nitrosodimethylamine (NDMA) is a sort of genotoxic impurities (GTIs) which has strong carcinogenic effect and obvious hepatotoxicity. To monitor NDMA content of in Metformin Hydrochloride Sustained-release Tablets and Enteric Capsules in China from 2018 to 2022, a GC-MS/MS method was established and verified. Methods:: The chromatographic column was Agilent VF-WAXms capillary column (30 m×0.25 mm, 0.25 μm). The GC-MS/MS method was equipped with multiple reaction monitoring (MRM) modes. To assess the quantity of NDMA, the molecular ion at mass-to-charge (m/z) of 74-44 was monitored under the 6 V collision energy, and to assess the quality of NDMA monitoring, the molecular ions at m/z 74-42 were determined. A total of 143 batches of metformin hydrochloride-finished products from 35 enterprises were determined by this method. Results:: The linear range of the method was 0.25 ~ 50.00 ng/mL, r = 0.9998, S/N>10, and the limit of detection and quantitation were 0.06 ng/mL and 0.21 ng/mL, respectively. The average recovery was 98.62%, and the RSD was 4.31%. All batches of enteric capsules met the requirements; 38.21% of the 123 batches sustained-release tablets still exceeded the acceptable daily intake. Conclusion:: The presented method is sensitive, accurate, precise, and available for both enteric capsules and sustained-release tablets of metformin hydrochloride, which can provide a reference for their quality control. The over-limit phenomenon of NDMA in metformin hydrochloride products poses new challenges and requirements for both the State Drug Administration and enterprises. result: The linear range of the method was 0.25 ~ 50.00 ng/mL, r = 0.9998, S/N>10, and the limit of detection was 0.21 ng/mL. The average recovery was 98.62 %, and the RSD was 4.31%. All batches of enteric capsules met the requirements, 38.21% of the 123 batches sustained-release tablets still exceeded the acceptable daily intake. other: nothing","PeriodicalId":10889,"journal":{"name":"Current Pharmaceutical Analysis","volume":"16 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135647190","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-10-01DOI: 10.2174/0115734129252205230920052737
Sohair Salah Ahmed, Ashraf Rasheed
Background:: Antiviral drugs are vital since many viruses can produce fatal infections, as we have recently seen with the COVID-19 pandemic. Antiviral drugs can battle viruses at multiple stages of their life cycles, including neuraminidase, nucleic acid synthesis, protease, and virion fusion or entry [1]. Objective:: Antiviral drugs have poor retention in reversed-phase liquid chromatography, which makes it difficult to analyze a mixture of antiviral medications using high-performance liquid chromatography. Using zwitterionic hydrophilic interaction liquid chromatography (ZICHILIC), the paper highlights the simultaneous quantification of three antiviral drugs as active constituents in pharmaceutical formulations. Moreover, the influence of the length of the spacer between the two charges in two stationary phases on the retention behavior of antiviral drugs has been discussed. Methods:: Two homemade stationary phases (ZIC1-HILIC and ZIC5-HILIC) were utilized for the qualitative and quantitative analysis of three antiviral drugs, and UV was used as the detector. Several chromatographic conditions were examined, such as the organic modifier concentration, buffer concentration, and pH value. Results:: After optimizing the parameters, the devised method was applied to analyse three antiviral medications quantitatively. The initial results demonstrated the current procedure for separating and determining these three antiviral drugs to be sensitive, robust, and effective. Consequently, the present method has shown excellent repeatability, a broad linear range (0.1- 16.5 μgml-1), and excellent sensitivity (LOD 0.04-0.072 μgml-1). The RSD value of the method was less than 1. Conclusion:: A mixed mode of hydrophilic and ion exchange interactions was the predominant mode of antiviral medications with two ZIC-HILIC stationary phases. The ZIC5-HILIC stationary phase had a lower detection and limit of quantitation for three antiviral drugs and a prolonged retention time compared to the ZIC1-HILIC stationary phase with a shorter chain length.
{"title":"Comparison of ZIC-HILIC Columns for the Simultaneous Analysis of Antiviral Drugs in Dosage Forms by Hydrophilic Interaction Liquid Chromatography","authors":"Sohair Salah Ahmed, Ashraf Rasheed","doi":"10.2174/0115734129252205230920052737","DOIUrl":"https://doi.org/10.2174/0115734129252205230920052737","url":null,"abstract":"Background:: Antiviral drugs are vital since many viruses can produce fatal infections, as we have recently seen with the COVID-19 pandemic. Antiviral drugs can battle viruses at multiple stages of their life cycles, including neuraminidase, nucleic acid synthesis, protease, and virion fusion or entry [1]. Objective:: Antiviral drugs have poor retention in reversed-phase liquid chromatography, which makes it difficult to analyze a mixture of antiviral medications using high-performance liquid chromatography. Using zwitterionic hydrophilic interaction liquid chromatography (ZICHILIC), the paper highlights the simultaneous quantification of three antiviral drugs as active constituents in pharmaceutical formulations. Moreover, the influence of the length of the spacer between the two charges in two stationary phases on the retention behavior of antiviral drugs has been discussed. Methods:: Two homemade stationary phases (ZIC1-HILIC and ZIC5-HILIC) were utilized for the qualitative and quantitative analysis of three antiviral drugs, and UV was used as the detector. Several chromatographic conditions were examined, such as the organic modifier concentration, buffer concentration, and pH value. Results:: After optimizing the parameters, the devised method was applied to analyse three antiviral medications quantitatively. The initial results demonstrated the current procedure for separating and determining these three antiviral drugs to be sensitive, robust, and effective. Consequently, the present method has shown excellent repeatability, a broad linear range (0.1- 16.5 μgml-1), and excellent sensitivity (LOD 0.04-0.072 μgml-1). The RSD value of the method was less than 1. Conclusion:: A mixed mode of hydrophilic and ion exchange interactions was the predominant mode of antiviral medications with two ZIC-HILIC stationary phases. The ZIC5-HILIC stationary phase had a lower detection and limit of quantitation for three antiviral drugs and a prolonged retention time compared to the ZIC1-HILIC stationary phase with a shorter chain length.","PeriodicalId":10889,"journal":{"name":"Current Pharmaceutical Analysis","volume":"138 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135661831","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-10-01DOI: 10.2174/0115734129255763230927115653
Kajal Baviskar, Ramanlal Kachave
Abstract: Sartans are often used as antihypertensives. They are also available in combination with thiazide diuretics for the management of hypertension. Analytical method development is a crucial part of successful drug development and characterization. Bioanalytical studies are of paramount importance while establishing pharmacokinetic and toxicokinetic data while forced degradation studies are important to elucidate degradation pathways and to establish stability of the drugs. : Different methods have been developed for the analysis of sartans and their combination with thiazide diuretics. We thought it imperative to summarize them so the data could be useful for analysis of newer sartans. The review describes various methods for analysis of some frequently employed sartans as well as the latest sartans and their combination with thiazide diuretics. The article also focuses on their analysis of biological fluids. Forced degradation studies have also been covered in the article. : Article is divided into three sections. First section covers introduction, second section focuses on different methods developed, including bioanalytical methods, while third section presents forced degradation studies carried out on the drugs. Important parameters of the analytical methods developed have been summarized in tabular form.
{"title":"Spectroscopic and Chromatographic Estimation of Some Sartans and their Combinations with Thiazide Diuretics: A Review","authors":"Kajal Baviskar, Ramanlal Kachave","doi":"10.2174/0115734129255763230927115653","DOIUrl":"https://doi.org/10.2174/0115734129255763230927115653","url":null,"abstract":"Abstract: Sartans are often used as antihypertensives. They are also available in combination with thiazide diuretics for the management of hypertension. Analytical method development is a crucial part of successful drug development and characterization. Bioanalytical studies are of paramount importance while establishing pharmacokinetic and toxicokinetic data while forced degradation studies are important to elucidate degradation pathways and to establish stability of the drugs. : Different methods have been developed for the analysis of sartans and their combination with thiazide diuretics. We thought it imperative to summarize them so the data could be useful for analysis of newer sartans. The review describes various methods for analysis of some frequently employed sartans as well as the latest sartans and their combination with thiazide diuretics. The article also focuses on their analysis of biological fluids. Forced degradation studies have also been covered in the article. : Article is divided into three sections. First section covers introduction, second section focuses on different methods developed, including bioanalytical methods, while third section presents forced degradation studies carried out on the drugs. Important parameters of the analytical methods developed have been summarized in tabular form.","PeriodicalId":10889,"journal":{"name":"Current Pharmaceutical Analysis","volume":"58 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135762577","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-10-01DOI: 10.2174/1573412919666230908161943
Akhil Gupta, Shilpi Pathak
Abstract: Herpes simplex virus (HSV) is a viral infection that primarily targets oral and genital organs in humans. Acyclovir is a widely prescribed anti-viral agent used in the infection caused by herpes simplex virus (HSV). This article emphasizes several analytical techniques, including spectrophotometry, High-performance liquid chromatography, High-performance thin-layer liquid chromatography, Ultra performance liquid chromatography, and Liquid chromatography/Mass detection for the quantification of acyclovir in different matrices like biological fluids and Pharmaceutical formulation. In the proposed work, numerous methods for different techniques were extracted from various databases such as Science Direct, Springer, PubMed, SCOPUS, Web of Science, etc. According to the recommendation from the internal conference on harmonization, this review describes how to determine the presence of utilizing acyclovir in different analytical techniques alone or in combination with another drug.
摘要:单纯疱疹病毒(HSV)是一种主要以口腔和生殖器官为靶点的病毒性感染。无环鸟苷是一种广泛使用的抗病毒药物，用于单纯疱疹病毒(HSV)引起的感染。本文着重介绍了分光光度法、高效液相色谱法、高效薄层液相色谱法、超高效液相色谱法、液相色谱/质谱法等分析技术在生物制剂和制剂等不同基质中对阿昔洛韦的定量分析。在本文中，我们从Science Direct、施普林格、PubMed、SCOPUS、Web of Science等数据库中提取了许多不同技术的方法。根据内部协调会议的建议，本综述描述了如何确定在不同的分析技术中单独或与另一种药物联合使用阿昔洛韦的存在。
{"title":"Analytical Methodologies for the Estimation of Acyclovir as Key Members of Anti-viral Agent: Two Decades in Review","authors":"Akhil Gupta, Shilpi Pathak","doi":"10.2174/1573412919666230908161943","DOIUrl":"https://doi.org/10.2174/1573412919666230908161943","url":null,"abstract":"Abstract: Herpes simplex virus (HSV) is a viral infection that primarily targets oral and genital organs in humans. Acyclovir is a widely prescribed anti-viral agent used in the infection caused by herpes simplex virus (HSV). This article emphasizes several analytical techniques, including spectrophotometry, High-performance liquid chromatography, High-performance thin-layer liquid chromatography, Ultra performance liquid chromatography, and Liquid chromatography/Mass detection for the quantification of acyclovir in different matrices like biological fluids and Pharmaceutical formulation. In the proposed work, numerous methods for different techniques were extracted from various databases such as Science Direct, Springer, PubMed, SCOPUS, Web of Science, etc. According to the recommendation from the internal conference on harmonization, this review describes how to determine the presence of utilizing acyclovir in different analytical techniques alone or in combination with another drug.","PeriodicalId":10889,"journal":{"name":"Current Pharmaceutical Analysis","volume":"26 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135219495","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-10-01DOI: 10.2174/0115734129263384230928052923
Miglena Smerikarova, Stanislav Bozhanov, Alexandrina Mateeva, Vania Maslarska
Background:: The onset of the COVID-19 pandemic caused numerous difficulties in the treatment of cardiovascular diseases and diabetes mellitus. A persistent risk of developing severe complications and increased mortality from the COVID-19 infection has been reported. In the clinical studies, patients receiving remdesivir and dexamethasone as COVID-19 combination therapy simultaneously with some type II diabetes therapeutic regimens had been reported to have a considerably better state and recover faster. Unfortunately, there is not enough information on the combination of meglitinides, remdesivir, and dexamethasone, and therefore, careful monitoring of the patients' everyday health condition is needed. Objectives:: The present study aimed to describe a high-performance liquid chromatographic method for the determination of repaglinide, dexamethasone, and remdesivir in laboratoryprepared mixtures and human plasma by UV detection. Methods:: Isocratic elution of the mobile phase (consisting of 0.1% trifluoroacetic acid in water and acetonitrile in the ratio 70:30 v/v) was set at a flow rate of 1.0 ml/min, and the developed analytical procedure has been found to be fast and simple. Chromatographic determination was performed on a Purospher® RP – 18 column at room temperature and a UV detector was set at 235 nm. result: The developed method was validated for linearity in the range 2-32 μg/ml. Calibration curves were linear over the selected range with correlation coefficients (R2) greater than 0.996. The coefficients of variation for intraday and interday assay were <2% and the recovery percentages from plasma ranged from 93.83 to 106.49%. Conclusion:: The developed effective and specific method can be applied in routine quality control and clinical laboratory practice.
{"title":"Development of Simple HPLC-UV Method for the Simultaneous Determination of Repaglinide, Dexamethasone, and Remdesivir, and its Application to Synthetic Mixture and Human Plasma","authors":"Miglena Smerikarova, Stanislav Bozhanov, Alexandrina Mateeva, Vania Maslarska","doi":"10.2174/0115734129263384230928052923","DOIUrl":"https://doi.org/10.2174/0115734129263384230928052923","url":null,"abstract":"Background:: The onset of the COVID-19 pandemic caused numerous difficulties in the treatment of cardiovascular diseases and diabetes mellitus. A persistent risk of developing severe complications and increased mortality from the COVID-19 infection has been reported. In the clinical studies, patients receiving remdesivir and dexamethasone as COVID-19 combination therapy simultaneously with some type II diabetes therapeutic regimens had been reported to have a considerably better state and recover faster. Unfortunately, there is not enough information on the combination of meglitinides, remdesivir, and dexamethasone, and therefore, careful monitoring of the patients' everyday health condition is needed. Objectives:: The present study aimed to describe a high-performance liquid chromatographic method for the determination of repaglinide, dexamethasone, and remdesivir in laboratoryprepared mixtures and human plasma by UV detection. Methods:: Isocratic elution of the mobile phase (consisting of 0.1% trifluoroacetic acid in water and acetonitrile in the ratio 70:30 v/v) was set at a flow rate of 1.0 ml/min, and the developed analytical procedure has been found to be fast and simple. Chromatographic determination was performed on a Purospher® RP – 18 column at room temperature and a UV detector was set at 235 nm. result: The developed method was validated for linearity in the range 2-32 μg/ml. Calibration curves were linear over the selected range with correlation coefficients (R2) greater than 0.996. The coefficients of variation for intraday and interday assay were <2% and the recovery percentages from plasma ranged from 93.83 to 106.49%. Conclusion:: The developed effective and specific method can be applied in routine quality control and clinical laboratory practice.","PeriodicalId":10889,"journal":{"name":"Current Pharmaceutical Analysis","volume":"211 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135762585","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-10-01DOI: 10.2174/0115734129255201230925103348
Neha Singh, Sumit Pannu, Karanvir Singh, Md Jawaid Akhtar, Ankit Anchliya, Shah Alam Khan
Abstract: The physicochemical properties of non-chromophoric compounds that lack a group to absorb UV-visible radiation make them difficult to analyze with a simple detector. Pharmaceutical formulations and their unknown impurities, which show weak or no response with a UV detector, remain undetected and pose a challenge to the analysis of these compounds. Direct measurement of a chromophore complex formed between the compound and the colored ions present in the electrolyte solution with UV detection is one of the validated methods to analyze non-chromophoric compounds. The derivatization with either chromophore or fluorescent group for the detection of the non-chromophoric compounds with HPLC-UV-Vis or fluorescence detector is also commonly used to study the physicochemical properties of the pharmaceutical formulations. The other techniques to analyze such non-chromophoric compounds include conductivity (ionic molecules), amperometry (molecules oxidized or reduced), mass spectrometry, evaporative light scattering detector (ELSD), condensation nucleation light scattering detector (CNLSD), capillary electrophoresis (CE), gas chromatography (GC), etc. This review covers various separation and detection techniques developed for the analysis of non-chromophoric compounds.
{"title":"Application of the Different Analytical Methods for Non-chromophoric Pharmaceutical Compounds","authors":"Neha Singh, Sumit Pannu, Karanvir Singh, Md Jawaid Akhtar, Ankit Anchliya, Shah Alam Khan","doi":"10.2174/0115734129255201230925103348","DOIUrl":"https://doi.org/10.2174/0115734129255201230925103348","url":null,"abstract":"Abstract: The physicochemical properties of non-chromophoric compounds that lack a group to absorb UV-visible radiation make them difficult to analyze with a simple detector. Pharmaceutical formulations and their unknown impurities, which show weak or no response with a UV detector, remain undetected and pose a challenge to the analysis of these compounds. Direct measurement of a chromophore complex formed between the compound and the colored ions present in the electrolyte solution with UV detection is one of the validated methods to analyze non-chromophoric compounds. The derivatization with either chromophore or fluorescent group for the detection of the non-chromophoric compounds with HPLC-UV-Vis or fluorescence detector is also commonly used to study the physicochemical properties of the pharmaceutical formulations. The other techniques to analyze such non-chromophoric compounds include conductivity (ionic molecules), amperometry (molecules oxidized or reduced), mass spectrometry, evaporative light scattering detector (ELSD), condensation nucleation light scattering detector (CNLSD), capillary electrophoresis (CE), gas chromatography (GC), etc. This review covers various separation and detection techniques developed for the analysis of non-chromophoric compounds.","PeriodicalId":10889,"journal":{"name":"Current Pharmaceutical Analysis","volume":"23 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136198950","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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