Current Pharmaceutical Analysis最新文献

{"title":"An Overview of Analytical Methods for the Identification and Quantification of Baclofen","authors":"João Augusto Oshiro-Junior, Milena Nogueira da Silva, João Victor Belo da Silva, Naara Felipe da Fonsêca, Ana Claudia Dantas Medeiros","doi":"10.2174/1573412919666230502124837","DOIUrl":"https://doi.org/10.2174/1573412919666230502124837","url":null,"abstract":"\u0000\u0000Baclofen is a potent antispasmodic agent, acting as an analgesic and central\u0000skeletal muscle relaxant. It is a GABA-B analog, and is widely used for the treatment of spasticity.\u0000Due to its therapeutic importance, various analytical techniques are used in the pharmaceutical industry and research to determine, identify, and characterize baclofen in bulk material, biological fluids,\u0000and pharmaceutical forms.\u0000\u0000\u0000\u0000This review aimed to collect information on reported analytical techniques commonly\u0000used to identify and quantify baclofen in pharmaceutical forms and biological samples.\u0000\u0000\u0000\u0000The authors explored various authenticated scientific journals using these descriptors: highperformance liquid chromatography, liquid chromatography-tandem mass spectrometry, capillary\u0000electrophoresis, differential scanning calorimetry, Fourier transform infrared spectroscopy, ultravioletvisible spectroscopy, near-infrared spectroscopy, nuclear magnetic resonance, potentiometry, and Xray diffraction.\u0000\u0000\u0000\u0000Quantification of the drug by all the methods evaluated in the review was possible. There\u0000were 73 articles reviewed, of which 26 used HPLC for baclofen quantification; the least used was near\u0000infrared spectroscopy and potentiometry, both with one article identified.\u0000\u0000\u0000\u0000This review has shed light on a wide variety of analytical methods that can be used to\u0000quantify and identify baclofen. The knowledge provided by the use of these analytical methods makes\u0000this document an important tool for developing pharmaceutical formulations containing baclofen.\u0000","PeriodicalId":10889,"journal":{"name":"Current Pharmaceutical Analysis","volume":" ","pages":""},"PeriodicalIF":0.6,"publicationDate":"2023-05-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45314297","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Experimental and Theoretical Study of Biosurfactants Functionalized Gold Nanoparticles for Mixture Detection and Chiral Recognition of Tryptophan by UV-Vis Spectroscopy","authors":"Lu Huang, Xiangzong Wu, Yanxia Li, Yiting Chen, Zhenli Qiu","doi":"10.2174/1573412919666230427110327","DOIUrl":"https://doi.org/10.2174/1573412919666230427110327","url":null,"abstract":"\u0000\u0000Tryptophan (Trp) is an essential amino acid and plays important roles in biological processes. The detection of Trp is very important for its biological and chemical study. Moreover, Trp is a chiral compound; due to its importance in biological processes, researchers have been\u0000long committed to the chiral recognition and sensing of Trp enantiomers.\u0000\u0000\u0000\u0000Two biosurfactants, sodium cholate and sodium deoxycholate, were used for the preparation of functionalized gold nanoparticles (AuNPs) which were characterized by transmission electron\u0000microscope and potentiometer. UV-Vis spectra of functionalized gold nanoparticle solutions with different concentrations of Trp, tyrosine, phenylalanine, D-Trp, and L-Trp were analyzed. Then, the discrimination mechanism was further investigated, and the promotion mechanism of biosurfactants was\u0000studied by density functional theory (DFT).\u0000\u0000\u0000\u0000Trp could induce the aggregation of unmodified AuNPs in 2 h, while phenylalanine and tyrosine could not. Adding biosurfactants promoted the aggregation process, and D- Trp rather than LTrp was found to be responsible for the aggregation. Therefore, there were interaction differences not\u0000only between Trp, phenylalanine, and tyrosine but also between Trp enantiomers.\u0000\u0000\u0000\u0000UV-vis spectroscopy could be applied for the direct detection of Trp in mixtures as well\u0000as the chiral recognition of Trp enantiomers. DFT calculations proved that the interactions of D-Trp\u0000with biosurfactants were the strongest, which contributes to the promotion of aggregation.\u0000","PeriodicalId":10889,"journal":{"name":"Current Pharmaceutical Analysis","volume":" ","pages":""},"PeriodicalIF":0.6,"publicationDate":"2023-04-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47131843","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The development and validation of simultaneous quantitative analysis reversed-phase high-performance liquid chromatography for sitagliptin phosphate monohydrate and dapagliflozin propanediol monohydrate fixed-dose combination dual-layered tablet","authors":"Joo-Eun Kim, So-Jin Kang","doi":"10.2174/1573412919666230417081123","DOIUrl":"https://doi.org/10.2174/1573412919666230417081123","url":null,"abstract":"\u0000\u0000Sitagliptin phosphate monohydrate-dapagliflozin propanediol hydrate fixeddose combination (FDC) dual-layered tablet is used for type 2 diabetes treatment. Simultaneous quantitative analysis can shorten the analysis time of sitagliptin phosphate monohydrate-dapagliflozin propanediol monohydrate FDC dual-layered tablets and increase their efficiency.\u0000\u0000\u0000\u0000This study aimed to develop the simultaneous quantitative analysis for sitagliptin phosphate\u0000monohydrate-dapagliflozin propanediol monohydrate FDC dual-layered tablet, a type 2 diabetes treatment.\u0000\u0000\u0000\u0000Simultaneous quantitative analysis using the rapid and selective reversed-phase highperformance liquid chromatography (RP-HPLC) method was developed and validated using method\u0000validation. RP-HPLC analysis was conducted using an ultraviolent absorption spectrophotometer and\u0000a Zorbax C18 column (4.6 x 150 mm, 5 µm). The flow rate and injection volume were set to 1.5 mL\u0000min-1 and 20 µL, respectively. The wavelength was set at 205 nm.\u0000\u0000\u0000\u0000The retention times of sitagliptin phosphate monohydrate and dapagliflozin propanediol\u0000monohydrate were 2.28 mins and 10.65 mins, respectively. The relative standard deviations of the\u0000system suitability for validation of simultaneous quantitative analysis were 0.03% for sitagliptin phosphate monohydrate and dapagliflozin propanediol monohydrate. The chromatogram confirmed that\u0000there was no peak interference between the two main components and between the main component\u0000and the excipients. In addition, It revealed a favorable linearity with correlation coefficients of 0.9999\u0000in the concentration range of 20–120% compared to the standard solution.\u0000\u0000\u0000\u0000The developed simultaneous quantitative analysis shortened the analysis time and high\u0000efficiency of the sitagliptin phosphate monohydrate-dapagliflozin propanediol monohydrate FDC bilayer tablet. The validity of the analytical method was verified through accuracy and precision, detection and quantitation limits, and solution stability tests. In addition, it was thought that it would be\u0000helpful in developing an analytical method by referring to the simultaneous quantitative analysis\u0000method for developing other FDC dual-layered tablets.\u0000","PeriodicalId":10889,"journal":{"name":"Current Pharmaceutical Analysis","volume":" ","pages":""},"PeriodicalIF":0.6,"publicationDate":"2023-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43203505","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Survey on Multi-omics, and Multi-omics Data Analysis, Integration and Application","authors":"","doi":"10.2174/1573412919666230406100948","DOIUrl":"https://doi.org/10.2174/1573412919666230406100948","url":null,"abstract":"\u0000\u0000Multi-omics approaches have developed as a profitable technique for plant systems, a\u0000popular method in medical and biological sciences underlining the necessity to outline new integrative technology and functions to facilitate the multi-scale depiction of biological systems. Understanding a biological system through various omics layers reveals supplementary sources of\u0000variability and probably inferring the sequence of cases leading to a definitive process. Manuscripts and reviews were searched on PubMed with the keywords of multi-omics, data analysis,\u0000omics, data analysis, data integration, deep learning multi-omics, and multi-omics integration. Articles that were published after 2010 were prioritized. The authors focused mainly on popular\u0000publications developing new approaches. Omics reveal interesting tools to produce behavioral\u0000and interactions data in microbial communities, and integrating omics details into microbial risk\u0000assessment will have an impact on food safety, and also on relevant spoilage control procedures.\u0000Omics datasets, comprehensively characterizing biological cases at a molecular level, are continually increasing in both dimensionality and complexity. Multi-omics data analysis is appropriate\u0000for treatment optimization, molecular testing and disease prognosis, and to achieve mechanistic\u0000understandings of diseases. New effective solutions for multi-omics data analysis together with\u0000well-designed components are recommended for many trials. The goal of this mini-review article\u0000is to introduce multi-omics technologies considering different multi-omics analyses.\u0000","PeriodicalId":10889,"journal":{"name":"Current Pharmaceutical Analysis","volume":" ","pages":""},"PeriodicalIF":0.6,"publicationDate":"2023-04-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47709311","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Validation of the T–47D Cell Culture Bioassay for the Potency Assessment of Botulinum Neurotoxin Type A","authors":"S. Dalmora, Bruna Xavier, Rafaela Ferreira Perobelli Dumoncel, Clóvis Dervil Appratto Cardoso Jr, F. S. da Silva","doi":"10.2174/1573412919666230320155755","DOIUrl":"https://doi.org/10.2174/1573412919666230320155755","url":null,"abstract":"\u0000\u0000Botulinum neurotoxins (BoNTs) are among the most potent toxins known and are also used for therapeutic and aesthetic applications.\u0000\u0000\u0000\u0000An alternative in vitro cell culture bioassay based on the induction of apoptosis on T−47D breast cancer cells, after exposure to BoNTA, was developed and validated.\u0000\u0000\u0000\u0000The T-47D cells (ATCC HTB−133) were seeded at a density of 3 × 105 cells mL−1, and the bioassay was performed with doses of BoNTA, between 3 and 81 U mL−1. The responses were assessed using 10 µL of Alamar Blue®. The absorbances were read at 570 and 600 nm.\u0000\u0000\u0000\u0000The results were compared with those of the in vivo LD50 mouse bioassay, showing a non-significant 1.08% higher, mean difference of the estimated potencies (p>0.05). Besides, the biopharmaceutics is analyzed by the size exclusion and reversed-phase liquid chromatography methods, showing a significant correlation with values 1.15% higher and 0.85% lower, respectively, related to the cell culture bioassay.\u0000\u0000\u0000\u0000It is concluded that the validated T−47D cell culture assay represents an advancement toward the establishment of an alternative approach for the potency assessment, in the context of the 3 Rs. Besides, the employment of chromatographic methods in conjunction with the bioassays contributes to assessing the quality attributes of the biopharmaceutical formulations of BoNTA.\u0000","PeriodicalId":10889,"journal":{"name":"Current Pharmaceutical Analysis","volume":" ","pages":""},"PeriodicalIF":0.6,"publicationDate":"2023-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47917653","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Stability Study and Simultaneous determination of Norepinephrine, Moxifloxacin, and Piperacillin + Tazobactam Mixtures applied in Intensive Care Medicine","authors":"J. Schmidt, M. Steppe","doi":"10.2174/1573412919666230315151351","DOIUrl":"https://doi.org/10.2174/1573412919666230315151351","url":null,"abstract":"\u0000\u0000In intensive care units intravenous medicine may be used in simultaneous infusion in the same intravenous site. Sometimes, the physical compatibility and stability of the combined solutions are unknown.\u0000\u0000\u0000\u0000The objective was to develop, optimize and validate a simple, fast and sensitive stability-indicating high-performance liquid chromatography (HPLC) for simultaneous quantification of binary mixtures of norepinephrine, piperacillin + tazobactam, moxifloxacin for intravenous (IV) administration in different diluents and physical compatibility with mannitol.\u0000\u0000\u0000\u0000The HPLC method was performed on a C18LUNA (4.6x250 mm 5-Micron) column, using acetonitrile: methanol: phosphate buffer pH 3.0 (20:30:50) as eluent and validated according to ICH guidelines and applied to mixtures of norepinephrine, moxifloxacin, piperacillin, tazobactam and mannitol at 0, 2, 6, 9 and 24 h. The substances and their mixtures were also evaluated by visual inspection and pH over time.\u0000\u0000\u0000\u0000The analytical method developed was specific, linear, precise, accurate and robust. No visual changes were observed in the mixtures over time, maintaining the pH values (except for piperacillin + tazobactam which changed 0.5 in 24 h) and losses of less than 10% of content over the 24 h under analyzed conditions.\u0000\u0000\u0000\u0000The proposed method is suitable for simultaneous analysis of norepinephrine, moxifloxacin, piperacillin and tazobactam. All tested mixtures were compatible and stable for up to 24 h, which is an important result for increasing patient safety in clinical practice since it has not been reported in the literature yet. The method can be further investigated and used for different concentration and diluent combinations.\u0000\u0000\u0000\u0000Conclusion: The proposed method is suitable for simultaneous analysis of norepinephrine, moxifloxacin, piperacillin and tazobactam. All tested mixtures were compatible and stable for up to 24 h, which is an important result for increase patient safety in clinical practice, since it has not been reported in literature yet. The method can be further investigated and used for different concentration and diluents combinations.\u0000\u0000\u0000\u0000HPLC\u0000","PeriodicalId":10889,"journal":{"name":"Current Pharmaceutical Analysis","volume":" ","pages":""},"PeriodicalIF":0.6,"publicationDate":"2023-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48854962","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Investigation of the Pharmacokinetic Properties and Theoretical Chemical Activities of 7,8-Dihydroxyflavone and 4'-Dimethylamino-7,8-Dihydroxyflavone","authors":"O. Korkmaz, M. F. Karakaya, Faik Gokalp, E. Şener","doi":"10.2174/1573412919666230313143549","DOIUrl":"https://doi.org/10.2174/1573412919666230313143549","url":null,"abstract":"\u0000\u0000Flavonoids naturally exist in plants as secondary metabolites. In this study, the aim is to determine and compare the theoretical and in vivo chemical activities of 7,8-dihydroxyflavone (7,8-DHF) and 4'dimethylamino-7,8-dihydroxyflavone (4’-DMA-7,8-DHF), tyrosine receptor kinase B (TrkB) receptor agonist flavonoid molecules with reported potent neuroprotective effects.\u0000\u0000\u0000\u0000BDNF has been thought to be a potent therapeutic agent against neurological disorders via its receptor TrkB. However, BDNF has poor pharmacokinetic properties and cannot cross the blood-brain barrier. It has been demonstrated that 7,8-DHF and 4''-DMA-7,8-DHF can bind and activate TrkB receptors and pass the blood-brain barrier. It has been thought that 4''-DMA-7,8-DHF may be more potent than 7,8-DHF due to strong TrkB activity and supporting neurogenesis at lower concentrations. However, there is no detailed study on this yet.\u0000\u0000\u0000\u0000method was used for the theoretical chemical analysis. For the in vivo studies, 6-month-old Wistar rats were used in two groups (n=8). 7,8-DHF and 4’-DMA-7,8-DHF (5 mg/kg) were administered intraperitoneally (ip) to each group. Then, plasma samples were collected by carotid catheterization, and brain samples by the microdialysis technique were collected simultaneously for 12 h from awake rats. The level of 7,8-DHF and 4’-DMA-7,8-DHF in blood and brain samples were analyzed and their pharmacokinetics were determined.\u0000\u0000\u0000\u0000Flavonoids naturally exist in plants as seconder metabolites. In this study, the aim is to determine and compare the theoretical and in vivo chemical activities of 7,8-DHF and 4’-DMA-7,8-DHF, tyrosine receptor kinase B (TrkB) receptor agonist flavonoid molecules with reported potent neuroprotective effects.\u0000\u0000\u0000\u0000Theoretical calculations show that 7,8-DHF is slightly more stable than 4’-DMA-7,8-DHF. The in vivo pharmacokinetic results show that the maximum concentration of 7,8-DHF was about 48 ng/mL, whereas it was only 8 ng/mL for 4’-DMA-7,8-DHF.\u0000\u0000\u0000\u0000Our results suggest that the 4'-DMA-7,8-DHF is more unstable and is more prone to binding to TrkB than 7,8-DHF. On the other hand, the in vivo pharmacokinetic results show that 7,8-DHF is more stable than 4’-DMA-7,8-DHF when it is applied systemically at therapeutic concentrations.\u0000\u0000\u0000\u0000Theoretical calculations show that 7,8-DHF is slightly more stable than 4’-DMA-7,8-DHF. The in vivo pharmacokinetic results show that the maximum concentration of 7,8-DHF was about 48 ng/mL, whereas it was only 8 ng/mL for 4’-DMA-7,8-DHF.\u0000\u0000\u0000\u00007.8-DHF seems more suitable for pharmacological applications.\u0000","PeriodicalId":10889,"journal":{"name":"Current Pharmaceutical Analysis","volume":" ","pages":""},"PeriodicalIF":0.6,"publicationDate":"2023-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47503732","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Metabolism of Portulacatone B from Portulaca oleracea L. in rats by UHPLC-ESI-Q-TOF/MS","authors":"Aijing Leng, Xixiang Ying, Xinyu Cui, Xiujuan Lan","doi":"10.2174/1573412919666230306124751","DOIUrl":"https://doi.org/10.2174/1573412919666230306124751","url":null,"abstract":"\u0000\u0000Objective: This study aims to investigate the main metabolites and metabolic pathways of Portulacatone B in rats, which is an alkaloid isolated from Portulaca oleracea L.\u0000\u0000\u0000\u0000Portulaca oleracea L. is an edible and medicinal plant belonging to the family of Portulacaceae, with rich pharmacological effects mainly attributed to its active ingredients. Studies have shown that Portulacatone B has excellent anti-inflammatory and anticholinesterase activity, which was further investigated.\u0000\u0000\u0000\u0000Portulacatone B was administered through the tail vein of the rat, and the orbital blood at 10 and 30 min and urine and feces within 24 h were collected. The metabolites and metabolic pathways in the rat were researched by ultra-high performance liquid chromatography-electrospray coupled with quadrupole-time of flight mass spectrometry (UHPLC-ESI-Q-TOF/MS).\u0000\u0000\u0000\u0000This study aims to investigate the main metabolites and metabolic pathways of Portulacatone B in rats, which was an alkaloid isolated from Portulaca oleracea L.\u0000\u0000\u0000\u0000The research results of the metabolites and metabolic pathways of Portulacatone B showed that after administration through the tail vein of rats, 3 metabolites were found in the plasma sample, 2 metabolites in the urine sample, and one metabolite in the feces sample. The main metabolic pathways were found to be oxidation, hydrolysis, methylation, glucuronidation, and sulfonation.\u0000\u0000\u0000\u0000Portulacatone B was administered through the tail vein of the rat, the orbital blood of 10 and 30 min and urine and feces within 24 h were collected. The metabolites and metabolic pathway in the rat were researched by ultra-high performance liquid chromatography electrospray coupled with quadrupole-time of flight mass spectrometry (UHPLC-ESI-Q-TOF/MS).\u0000\u0000\u0000\u0000Six metabolites were found in the rat’s plasma, urine, and feces samples, and the metabolic pathways included oxidation, hydrolysis, methylation, glucuronidation, and sulfonation process.\u0000\u0000\u0000\u0000The research results of the metabolites and metabolic pathways of Portulacatone B showed that after administration through the tail vein of rats, 3 metabolites were found in the plasma sample; 2 metabolites in the urine sample, and a metabolite in the feces sample. The main metabolic pathways are oxidation, hydrolysis, methylation, glucuronidation, and sulfonation.\u0000\u0000\u0000\u0000not\u0000","PeriodicalId":10889,"journal":{"name":"Current Pharmaceutical Analysis","volume":" ","pages":""},"PeriodicalIF":0.6,"publicationDate":"2023-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48617871","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A REVIEW: DRUG EXCIPIENT IINCOMPATIBLITY BY FTIR SPECTROSCOPY","authors":"S. Kumari, Chander Singh, K. Rao, Nikita Yadav, Nidhi Bansal, Yogesh Vashist, Palak Chugh","doi":"10.2174/1573412919666230228102158","DOIUrl":"https://doi.org/10.2174/1573412919666230228102158","url":null,"abstract":"\u0000\u0000For the development of various formulations, it is necessary to check out the drug excipient incompatibility. Whether the drug is compatible with the excipient or not. Because the drug excipient interaction study provides stability data of the drug and shelf life of the drug. Fourier transform infrared spectroscopy is the best method to evaluate the drug excipient incompatibility study. The FTIR spectroscopy theory is based on the idea that molecules have a tendency to absorb particular light frequencies that are unique to the corresponding structure of the molecules. The energies depend on the atomic masses, the related vibronic coupling, and the geometry of the molecular surfaces. For instance, the molecule may be able to absorb the energy present in the incident light, which will cause it to rotate more quickly or vibrate more loudly. In this article, a list of various drugs with different excipients was discussed. This review emphasizes on various examples of drug interaction with a number of excipients on the basis of Fourier Transform infrared spectroscopy data which is based on last 10-12 year research paper, and the principle ,working, applications of infrared spectroscopy were also discussed.\u0000","PeriodicalId":10889,"journal":{"name":"Current Pharmaceutical Analysis","volume":" ","pages":""},"PeriodicalIF":0.6,"publicationDate":"2023-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47401152","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pharmacokinetics and Tissue Distribution Study of Daphnoretin in Ethanol Extract from the Roots of Wikstroemia Indica in Rats by a Validated UPLC-MS/MS Method","authors":"Guo Feng, Wenjing Wang, Lai-lai Li, Wei Li, Wen Liu, Zeng-guang Wu, Hongmei Su, G. Zhu, Chenchen Ren, Xueli Song, Ju Zhang, Zhengyan He","doi":"10.2174/1573412919666230223140457","DOIUrl":"https://doi.org/10.2174/1573412919666230223140457","url":null,"abstract":"\u0000\u0000Daphnoretin, as a known bicoumarin compound that contained various pharmacological activities, was isolated from Wikstroemia indica C.A. Mey (RWI).\u0000\u0000\u0000\u0000The study aims to investigate the pharmacokinetic characteristics of daphnoretin from RWI ethanol extracts in rat plasma and to determine daphnetin in rat plasma and various tissues by a rapid, reliable and sensitive ultra high performance liquid chromatography with tandem mass spectrometry method.\u0000\u0000\u0000\u0000The UPLC-MS/MS method was established. Daphnoretin and IS (buspirone) were chromatographed on an agilent Zorbax XDB-C18 column (2.1 mm × 50 mm, 3.5 μm), and Gradient elution of acetonitrile-0.15% formic acid in aqueous solution.\u0000Quantification was performed using electrospray ionization in positive ion multiple reaction monitoring mode of the transitions m/z 353.1→179.1 for daphnoretin and m/z 386.3→122.3 for IS.\u0000\u0000\u0000\u0000Good linearity between 5–10000 ng/mL for cyperidin in plasma and tissue samples (r ≥ 0.99) was resulted. The accuracies of plasma and tissue homogenates ranged from –3.31% to 9.00%, and the precision was less than 5.78%. After that, the validated method was successfully applied to the pharmacokinetics and tissue distribution study of daphnoretin after oral administration of ethanol extract from the roots of RWI to rats.\u0000\u0000\u0000\u0000Daphnoretin was well absorbed in the systemic circulation after oral administration and was widely distributed in tissues, with the highest concentration in lung tissue. This study is beneficial to the development and utilization of RWI and provides a reasonable reference for its clinical administration.\u0000\u0000\u0000\u0000none\u0000","PeriodicalId":10889,"journal":{"name":"Current Pharmaceutical Analysis","volume":" ","pages":""},"PeriodicalIF":0.6,"publicationDate":"2023-02-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48726586","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}