Pub Date : 2019-06-01DOI: 10.35732/CTLABP.2019.5.1.27
Ja-I Kim, Seon-Gyu Kim, G. Moon
Nowadays micro-dust is a serious problem in Korea. In particular micro-dust contains heavy metals such as Pb (lead) and Cd (cadmium) which negatively effect on human health. In this study, we intended to isolate lactic acid bacteria which can scavenge the heavy metals. Firstly we isolated two lactic acid bacteria which were resistant to Ag, Cu, and Zn (30-100 mM AgNO 3, CuSO 4, ZnSO 4 in MRS broth). The two lactic acid bacteria CJNU 1877 and JG 15 were identified Lactobacillus paracasei and Enterococcus faecium, respectively. Subsequently the strains were inoculated in MRS broth and agar plate where 100 ppm of Pb(NO 3 ) 2 and CdSO 4 were added, respectively. They did grow in the conditions and we found aggregations from 24 h cultures, indicating the strains can absorb the heavy metals, which was further proved by scanning electron microscopy (SEM) and Inductively Coupled Plasma-Optical Emission Spectrometer. Therefore the isolated lactic acid bacteria can be used as a probiotics harboring heavy metal scavenging activity.
目前，微尘在韩国是一个严重的问题。特别是微尘中含有Pb(铅)和Cd(镉)等重金属，对人体健康产生负面影响。本研究旨在分离具有清除重金属功能的乳酸菌。首先分离出2株耐银、铜、锌(MRS肉汤中30-100 mM agno3、cuso4、znso4)的乳酸菌。经鉴定，乳酸菌cnjnu 1877和乳酸菌jg15分别为副干酪乳杆菌和屎肠球菌。随后将菌株接种于MRS肉汤和琼脂板上，分别添加100 ppm的Pb(no3) 2和cdso4。它们确实在这种条件下生长，并且我们在培养24 h时发现了聚集，表明菌株可以吸收重金属，扫描电子显微镜(SEM)和电感耦合等离子体光学发射光谱仪进一步证实了这一点。因此，分离得到的乳酸菌可作为一种具有清除重金属活性的益生菌。
{"title":"Isolation of Heavy Metal Scavenging Lactic Acid\u0000 Bacteria","authors":"Ja-I Kim, Seon-Gyu Kim, G. Moon","doi":"10.35732/CTLABP.2019.5.1.27","DOIUrl":"https://doi.org/10.35732/CTLABP.2019.5.1.27","url":null,"abstract":"Nowadays micro-dust is a serious problem in Korea. In particular micro-dust contains heavy metals such as Pb (lead) and Cd (cadmium) which negatively effect on human health. In this study, we intended to isolate lactic acid bacteria which can scavenge the heavy metals. Firstly we isolated two lactic acid bacteria which were resistant to Ag, Cu, and Zn (30-100 mM AgNO 3, CuSO 4, ZnSO 4 in MRS broth). The two lactic acid bacteria CJNU 1877 and JG 15 were identified Lactobacillus paracasei and Enterococcus faecium, respectively. Subsequently the strains were inoculated in MRS broth and agar plate where 100 ppm of Pb(NO 3 ) 2 and CdSO 4 were added, respectively. They did grow in the conditions and we found aggregations from 24 h cultures, indicating the strains can absorb the heavy metals, which was further proved by scanning electron microscopy (SEM) and Inductively Coupled Plasma-Optical Emission Spectrometer. Therefore the isolated lactic acid bacteria can be used as a probiotics harboring heavy metal scavenging activity.","PeriodicalId":10892,"journal":{"name":"Current Topic in Lactic Acid Bacteria and Probiotics","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81934796","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-06-01DOI: 10.35732/CTLABP.2019.5.1.13
J. Shin, Na Ri Kim, J. Koh, Jae-hwan Kim
Oligossaccharides have beneficial effects on the immune system, atopy and skin health according to the prebiotic effect on intestinal microflora. Due to the excellent physiological functions of these oligosaccharides, the demand for prebiotics is rapidly increasing in the world. In this review, it has been shown that galactooligo-saccharide(GOS) intake improves skin health and atopic eczema and fructooligo-saccharide(FOS) prevent atopic dermatitis in human clinical trials. These results indicate that oligosaccharides are sufficiently applicable as a functional food for improving skin health and skin diseases.
{"title":"Improvement Effect of Skin Health and Skin Disease of\u0000 Oligosaccharides","authors":"J. Shin, Na Ri Kim, J. Koh, Jae-hwan Kim","doi":"10.35732/CTLABP.2019.5.1.13","DOIUrl":"https://doi.org/10.35732/CTLABP.2019.5.1.13","url":null,"abstract":"Oligossaccharides have beneficial effects on the immune system, atopy and skin health according to the prebiotic effect on intestinal microflora. Due to the excellent physiological functions of these oligosaccharides, the demand for prebiotics is rapidly increasing in the world. In this review, it has been shown that galactooligo-saccharide(GOS) intake improves skin health and atopic eczema and fructooligo-saccharide(FOS) prevent atopic dermatitis in human clinical trials. These results indicate that oligosaccharides are sufficiently applicable as a functional food for improving skin health and skin diseases.","PeriodicalId":10892,"journal":{"name":"Current Topic in Lactic Acid Bacteria and Probiotics","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84780877","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-06-01DOI: 10.35732/CTLABP.2019.5.1.1
Hoon Park
Ginseng, the root of the Panax ginseng, has been widely used as a traditional herbal medicine in Korea, China, and Japan for thousands of years. Now ginseng has become popular as functional health food and natural medicine and it is one of the bestselling natural products in the world. Gut microbiota has been found to play an important role in the metabolism and pharmacological action of orally administered ginseng. Recent animal and clinical studies have shown that compound K (20-O -beta-D-glucopyranosyl-20(S)-protopanaxadiol, CK) is the major ginsenoside metabolite deriving from gut microbiota-induced biotransformation and is more efficiently absorbed into the systemic circulation than its parent ginsenosides. Since ginsenoside metabolism varies between individuals depending on the population of gut microbiota, much attention paid to the transformation of major ginsenosides into more pharmacologically active ginsenosides using diverse methods including heating, acid hydrolysis, microbial conversion, and enzymatic treatment. Recently, in food and drug industry and academia, the development of fermented ginseng products using probiotic bacteria is being intensively studied due to the potential health benefits of ginsenoside metabolites and probiotics. This review summarizes recent studies on the metabolism of ginsenoside by gut microbiota and biotransformation of ginsenoside using lactic acid bacteria and their enzymes.
人参是人参的根，在韩国、中国、日本作为传统草药被广泛使用了数千年。目前，人参作为功能性保健食品和天然药物受到广泛欢迎，是世界上最畅销的天然产品之一。肠道微生物群已被发现在口服人参的代谢和药理作用中起重要作用。最近的动物和临床研究表明，化合物K (20-O - β - d -glucopyranosyl-20(S)-protopanaxadiol, CK)是由肠道微生物诱导的生物转化产生的主要人参皂苷代谢物，比其母体人参皂苷更有效地吸收到体循环中。由于人参皂苷在个体之间的代谢因肠道微生物群的不同而不同，因此通过加热、酸水解、微生物转化和酶处理等多种方法将主要人参皂苷转化为更具药理活性的人参皂苷受到了广泛关注。近年来，由于人参皂苷代谢物和益生菌的潜在健康益处，在食品药品行业和学术界，利用益生菌开发发酵人参产品正在得到广泛研究。本文综述了近年来肠道菌群对人参皂苷的代谢和乳酸菌及其酶对人参皂苷的生物转化研究进展。
{"title":"The Role of Gut Microbiota in Ginsenoside Metabolism and\u0000 Biotransformation of Ginsenoside by Lactic Acid Bacteria","authors":"Hoon Park","doi":"10.35732/CTLABP.2019.5.1.1","DOIUrl":"https://doi.org/10.35732/CTLABP.2019.5.1.1","url":null,"abstract":"Ginseng, the root of the Panax ginseng, has been widely used as a traditional herbal medicine in Korea, China, and Japan for thousands of years. Now ginseng has become popular as functional health food and natural medicine and it is one of the bestselling natural products in the world. Gut microbiota has been found to play an important role in the metabolism and pharmacological action of orally administered ginseng. Recent animal and clinical studies have shown that compound K (20-O -beta-D-glucopyranosyl-20(S)-protopanaxadiol, CK) is the major ginsenoside metabolite deriving from gut microbiota-induced biotransformation and is more efficiently absorbed into the systemic circulation than its parent ginsenosides. Since ginsenoside metabolism varies between individuals depending on the population of gut microbiota, much attention paid to the transformation of major ginsenosides into more pharmacologically active ginsenosides using diverse methods including heating, acid hydrolysis, microbial conversion, and enzymatic treatment. Recently, in food and drug industry and academia, the development of fermented ginseng products using probiotic bacteria is being intensively studied due to the potential health benefits of ginsenoside metabolites and probiotics. This review summarizes recent studies on the metabolism of ginsenoside by gut microbiota and biotransformation of ginsenoside using lactic acid bacteria and their enzymes.","PeriodicalId":10892,"journal":{"name":"Current Topic in Lactic Acid Bacteria and Probiotics","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86235791","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-06-01DOI: 10.35732/CTLABP.2019.5.1.20
Sin-Young Park, Gye-Woong Kim, J. M. Kim, J. Koh, J. Choe, Hack-Youn Kim
This study evaluated the effects of probiotics on the quality properties of standard pork cutlet (SC) and oven cooking type pork cutlet with probiotics (OCP). Higher (p ＜0.0001) moisture and protein contents were observed in OCP compared to SC. Fat content of OCP was lower (p＜0.0001) than SC. There was no significant difference in CIE color values between SC and OCP. The pH and lipid oxidation values of OCP were lower (p＜0.0001 and p＜0.01, respectively) than SC counterpart. Based on instrumental texture profile analysis, there was no significant difference in the springiness and cohesiveness between SC and OCP. However, the hardness, gumminess, and chewiness of OCP were higher (p＜0.0001) than SC. Crispiness by sensory panel was higher (p＜0.0001) in OCP than SC. The results showed that the oven cooking type pork cutlet added probiotics could improve potential of pork cutlet physicochemical properties and give physiological functional ability.
{"title":"Comparative Analysis on the Quality Characteristics between Oven\u0000 Cooking Type Pork Cutlet Added Probiotics and Commercial Pork\u0000 Cutlet","authors":"Sin-Young Park, Gye-Woong Kim, J. M. Kim, J. Koh, J. Choe, Hack-Youn Kim","doi":"10.35732/CTLABP.2019.5.1.20","DOIUrl":"https://doi.org/10.35732/CTLABP.2019.5.1.20","url":null,"abstract":"This study evaluated the effects of probiotics on the quality properties of standard pork cutlet (SC) and oven cooking type pork cutlet with probiotics (OCP). Higher (p ＜0.0001) moisture and protein contents were observed in OCP compared to SC. Fat content of OCP was lower (p＜0.0001) than SC. There was no significant difference in CIE color values between SC and OCP. The pH and lipid oxidation values of OCP were lower (p＜0.0001 and p＜0.01, respectively) than SC counterpart. Based on instrumental texture profile analysis, there was no significant difference in the springiness and cohesiveness between SC and OCP. However, the hardness, gumminess, and chewiness of OCP were higher (p＜0.0001) than SC. Crispiness by sensory panel was higher (p＜0.0001) in OCP than SC. The results showed that the oven cooking type pork cutlet added probiotics could improve potential of pork cutlet physicochemical properties and give physiological functional ability.","PeriodicalId":10892,"journal":{"name":"Current Topic in Lactic Acid Bacteria and Probiotics","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80726665","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-06-01DOI: 10.35732/CTLABP.2019.5.1.33
Dong-Hun Chu, Moochang Kook
Lactobacillus pentosus A67 and L. planarum subsp. plantarum C2 was tested the probiotics characterization such as acid, bile acid and antibiotics resistance. Isolates were isolated from the traditional Korean food, collected from traditional markets and homes in Seoul and Gyeonggi Province, and were selected the isolates A67 and C2, which were the most antibacterial to E. coli. According to the analysis of 16S rRNA base sequences, the results showed that L. pentosus ATCC 8041 (similarity 100%), and L. plantarum subsp. plantarum ATCC 14917 (similarity 100%). L. pentosus A67 showed a survival rate of about 98.35% and the L. plantarum C2 showed a survival rate of about 95.62% at pH 2.5, respectively. L. pentosus A67 showed a survival rate of about 94.56% and the L. plantarum C2 showed a survival rate of about 88.81% on the 0.3% bile salt, respectively. L. pentosus A67 and L. plantarum C2 were survived by antibiotics such as the quinolone, aminoglycoside, macrolide, glycopeptide, tetracycline, cephalosporin and phenicillin families. On basis of these results, L. pentosus A67 and L. plantarum C2 are the candidates for useful probiotics.
戊酸乳杆菌A67与扁平乳杆菌亚种。对植物C2进行了酸、胆汁酸及抗生素耐药性等益生菌鉴定。从首尔和京畿地区的传统市场和家庭采集的韩国传统食品中分离出菌株，筛选出对大肠杆菌抑菌力最强的菌株A67和C2。根据16S rRNA碱基序列分析，结果表明，L. pentosus ATCC 8041(相似度100%)与L. plantarum subsp;plantarum ATCC 14917(相似性100%)。在ph2.5条件下，L. pentosus A67和L. plantarum C2的存活率分别为98.35%和95.62%。L. pentosus A67和L. plantarum C2在0.3%胆汁盐上的存活率分别为94.56%和88.81%。L. pentosus A67和L. plantarum C2主要通过喹诺酮类、氨基糖苷类、大环内酯类、糖肽类、四环素类、头孢菌素类和青霉素类抗生素存活。基于这些结果，戊糖乳杆菌A67和植物乳杆菌C2是有益益生菌的候选菌。
{"title":"Probiotics Characterization of Lactobacillus\u0000 Species Isolated from Kimchi","authors":"Dong-Hun Chu, Moochang Kook","doi":"10.35732/CTLABP.2019.5.1.33","DOIUrl":"https://doi.org/10.35732/CTLABP.2019.5.1.33","url":null,"abstract":"Lactobacillus pentosus A67 and L. planarum subsp. plantarum C2 was tested the probiotics characterization such as acid, bile acid and antibiotics resistance. Isolates were isolated from the traditional Korean food, collected from traditional markets and homes in Seoul and Gyeonggi Province, and were selected the isolates A67 and C2, which were the most antibacterial to E. coli. According to the analysis of 16S rRNA base sequences, the results showed that L. pentosus ATCC 8041 (similarity 100%), and L. plantarum subsp. plantarum ATCC 14917 (similarity 100%). L. pentosus A67 showed a survival rate of about 98.35% and the L. plantarum C2 showed a survival rate of about 95.62% at pH 2.5, respectively. L. pentosus A67 showed a survival rate of about 94.56% and the L. plantarum C2 showed a survival rate of about 88.81% on the 0.3% bile salt, respectively. L. pentosus A67 and L. plantarum C2 were survived by antibiotics such as the quinolone, aminoglycoside, macrolide, glycopeptide, tetracycline, cephalosporin and phenicillin families. On basis of these results, L. pentosus A67 and L. plantarum C2 are the candidates for useful probiotics.","PeriodicalId":10892,"journal":{"name":"Current Topic in Lactic Acid Bacteria and Probiotics","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76896079","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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