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Synergistic ice - Inhibition: revolutionizing cryopreservation via engineering approaches 协同抑冰:通过工程方法革新低温保存
IF 2.1 3区 生物学 Q2 BIOLOGY Pub Date : 2025-12-01 Epub Date: 2025-11-25 DOI: 10.1016/j.cryobiol.2025.105349
Gang Zhao
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引用次数: 0
Intracellular glass fragility distinguishes the desiccation response of embryonic axes from recalcitrant and orthodox seeds 细胞内玻璃脆性区分了胚胎轴与顽固性和正统种子的干燥反应
IF 2.1 3区 生物学 Q2 BIOLOGY Pub Date : 2025-12-01 Epub Date: 2025-11-25 DOI: 10.1016/j.cryobiol.2025.105348
Davide Gerna , Louise Colville , Daniel Ballesteros
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引用次数: 0
Improving drone sperm cryopreservation: Investigating cryoprotectant combinations and PVP supplementation 改进无人机精子冷冻保存:研究冷冻保护剂组合和PVP补充
IF 2.1 3区 生物学 Q2 BIOLOGY Pub Date : 2025-12-01 Epub Date: 2025-09-12 DOI: 10.1016/j.cryobiol.2025.105318
Abdulkadir Kaya , Ongun Uysal , Numan Akyol
Cryopreservation of drone sperm is a widely studied method for long-term genetic preservation and offers key advantages. However, despite successful cryopreservation, fertilization rates remain unsatisfactory. Artificial insemination with cryopreserved sperm reduces spermatozoa concentration in the queen's spermatheca, highlighting the need for alternative cryoprotectants and extenders. Research on other species suggests that combining cryoprotectants may enhance preservation outcomes compared to using them individually. This study evaluated the effects of various cryoprotectant combinations on drone sperm quality after cryopreservation. Phase I assessed the individual and combined effects of dimethyl sulfoxide (DMSO), glycerol (GLY), dimethylacetamide (DMA), and ethylene glycol (EG). In Phase II, different concentrations of polyvinylpyrrolidone (PVP) were added to DMSO. Finally, the three best combinations were tested in vivo for sperm concentration and plasma membrane integrity (PMI) in queen spermathecae following artificial insemination. DMSO-based cryoprotectants, particularly combinations with EG, GLY, and PVP, significantly enhanced post-thaw sperm quality (P ≤ 0.001). In Phase I, DMSO, EG, and DMSO + EG yielded the highest motility and PMI values. In Phase II, DMSO+6 % PVP achieved optimal results in all parameters. In Phase III, although all cryopreserved groups showed lower spermathecal sperm concentration and PMI compared to fresh semen, the DMSO + PVP group performed best compared to the other cryopreserved treatments. In conclusion, the combined use of cryoprotectants yielded superior cryopreservation outcomes compared to their individual utilization. DMSO showed greater efficacy than GLY, DMA, and EG, while PVP supplementation showed promise in improving drone sperm cryopreservation, warranting further investigation with expanded parameters.
低温保存雄蜂精子是一种被广泛研究的长期遗传保存方法,具有重要的优势。然而,尽管冷冻保存成功,受精率仍然令人不满意。用冷冻保存的精子进行人工授精会降低女王精子囊中的精子浓度,突出了对替代冷冻保护剂和扩展剂的需求。对其他物种的研究表明,与单独使用相比,联合使用冷冻保护剂可以提高保存效果。本研究评估了不同冷冻保护剂组合对低温保存后雄蜂精子质量的影响。第一阶段评估了二甲基亚砜(DMSO)、甘油(GLY)、二甲乙酰胺(DMA)和乙二醇(EG)的单独和联合效应。在第二阶段,在DMSO中加入不同浓度的聚乙烯吡咯烷酮(PVP)。最后,对三种最佳组合进行了人工授精后精囊内精子浓度和质膜完整性(PMI)的体内测定。基于dmso的冷冻保护剂,特别是与EG、GLY和PVP联合使用,显著提高了解冻后精子质量(P≤0.001)。在第一阶段,DMSO、EG和DMSO + EG产生最高的运动性和PMI值。在第二阶段,DMSO+ 6% PVP在所有参数上都达到了最佳效果。在III期试验中,尽管所有冷冻保存组的精囊精子浓度和PMI均低于新鲜精液,但DMSO + PVP组的表现优于其他冷冻保存处理。总之,与单独使用相比,联合使用冷冻保护剂获得了更好的冷冻保存结果。DMSO比GLY、DMA和EG更有效,而PVP补充剂在改善无人机精子冷冻保存方面有希望,值得进一步研究扩大参数。
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引用次数: 0
Research on the role of endoplasmic reticulum-mitochondria interactions in maintaining calcium homeostasis in skeletal muscle fibers of hibernating Daurian ground squirrels 内质网-线粒体相互作用在达斡尔地松鼠冬眠骨骼肌纤维钙稳态维持中的作用研究。
IF 2.1 3区 生物学 Q2 BIOLOGY Pub Date : 2025-12-01 Epub Date: 2025-10-02 DOI: 10.1016/j.cryobiol.2025.105337
Jie Zhang , Fangyang Pan , Yue He , Jing Zhao , Xiaozhuo Bai , Yanhong Wei , Yunfang Gao
The ability to maintain cytoplasmic Ca2+ homeostasis during the torpor-arousal cycle is a crucial strategy that enables hibernators to resist disuse-induced muscle atrophy during hibernation. However, whether ER-mitochondria interactions contribute to the regulation of cytoplasmic Ca2+ homeostasis in skeletal muscle fibers during hibernation remain unclear. Here, we systematically explored the expression of tethering proteins, Ca2+ axis-related proteins, and protein complexes involved in Ca2+ transport within the ER-mitochondria contact sites (ERMCS) of soleus (SOL) and extensor digitorum longus (EDL) muscles in Daurian ground squirrels (Spermophilus dauricus) during hibernation. Results demonstrated a significant increase in the expression of the tethering protein MFN2 and Ca2+ axis-related proteins GRP75 and VDAC1, as well as enhanced interactions within the Ca2+ axis complex in the SOL muscle in the inter-bout arousal (IBA) group, as compared to the late torpor (LT) group. In addition, these same indicators exhibited no significant alternation in the EDL muscle during the torpor-arousal cycle. These findings suggest that ER-mitochondria interactions may participate in maintaining Ca2+ homeostasis in the skeletal muscles during the torpor-arousal cycle, especially in slow-twitch muscle.
在冬眠-觉醒周期中维持细胞质Ca2+稳态的能力是使冬眠动物在冬眠期间抵抗废用诱导的肌肉萎缩的关键策略。然而,er -线粒体相互作用是否有助于冬眠期间骨骼肌纤维胞质Ca2+稳态的调节仍不清楚。在这里,我们系统地探讨了达斡尔地松鼠(达斡尔鼠)冬眠期间比目鱼肌(SOL)和指长伸肌(EDL) er -线粒体接触位点(ERMCS)内系栓蛋白、Ca2+轴相关蛋白和参与Ca2+运输的蛋白复合物的表达。结果表明,与晚睡(LT)组相比,在回合间唤醒(IBA)组中,SOL肌肉中栓系蛋白MFN2和Ca2+轴相关蛋白GRP75和VDAC1的表达显著增加,Ca2+轴复合物内的相互作用增强。此外,在冬眠-觉醒周期中,这些相同的指标在EDL肌中没有显着变化。这些发现表明,er -线粒体的相互作用可能参与维持骨骼肌在冬眠-觉醒周期中的Ca2+稳态,特别是在慢抽搐肌中。
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引用次数: 0
Effect of prolonged cooling on ovarian tissues 长时间冷却对卵巢组织的影响
IF 2.1 3区 生物学 Q2 BIOLOGY Pub Date : 2025-12-01 Epub Date: 2025-11-25 DOI: 10.1016/j.cryobiol.2025.105358
Emmerson Teas, Kate Fowler, Nilay Kuscu, Peter Kilbride, Suzannah A. Williams
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引用次数: 0
Confounding experimental variables in measuring cell volume osmotic responses relevant to cryopreservation 在测量与冷冻保存相关的细胞体积渗透反应时混淆实验变量
IF 2.1 3区 生物学 Q2 BIOLOGY Pub Date : 2025-12-01 Epub Date: 2025-11-25 DOI: 10.1016/j.cryobiol.2025.105364
Faranak Yadegari , Janet A.W. Elliott
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引用次数: 0
Cryopreservation of porcine intact tibial plateau using vitrification 玻璃化法冷冻保存猪胫骨平台
IF 2.1 3区 生物学 Q2 BIOLOGY Pub Date : 2025-12-01 Epub Date: 2025-11-25 DOI: 10.1016/j.cryobiol.2025.105399
Korinne M. Tirones , Mohammadhamed Shahsavari , Evan J.C. Andrin , Janet A.W. Elliott , Nadr M. Jomha
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引用次数: 0
Cold, chaos, confinement: On the evolving thermodynamics of modern cryopreservation 冷、混沌、约束:论现代低温保存热力学的演变
IF 2.1 3区 生物学 Q2 BIOLOGY Pub Date : 2025-12-01 Epub Date: 2025-11-25 DOI: 10.1016/j.cryobiol.2025.105381
Matthew J. Powell-Palm , Anthony N. Consiglio
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引用次数: 0
Spray freeze-drying of inhalable monoclonal antibodies: Enhancing stability and flowability 可吸入性单克隆抗体的喷雾冷冻干燥:增强稳定性和流动性
IF 2.1 3区 生物学 Q2 BIOLOGY Pub Date : 2025-12-01 Epub Date: 2025-11-25 DOI: 10.1016/j.cryobiol.2025.105378
Lorena Pasero , Adamo Sulpizi , Tomaso Guidi , Roberto Pisano
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引用次数: 0
Trehalose based RBC-preservation – From freezing to potential freeze-drying formulations 海藻糖为基础的红细胞保存-从冷冻到潜在的冷冻干燥配方
IF 2.1 3区 生物学 Q2 BIOLOGY Pub Date : 2025-12-01 Epub Date: 2025-11-25 DOI: 10.1016/j.cryobiol.2025.105405
Tobias Braun, Inola-Trinity Kohrs, Harriëtte Oldenhof, Willem F. Wolkers
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引用次数: 0
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