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Identifying Proteins Associated with Disease Severity 鉴定与疾病严重程度相关的蛋白质
O. Samarawickrama, R. Jayatillake, D. Amaratunga
Proteomic studies or studies of protein expression levels are growing swiftly with the steady improvement in technology and knowledge on understanding various anomalies affecting humans. Since differentially expressed proteins have an influence on overall cell functionality, this improves discrimination between healthy and diseased states. Identifying prime proteins offers prospective insights for developing optimized and targeted treatment methods. This research involves analyzing data from an early-stage study whose main purpose was to identify differentially expressed proteins. The presence of 3 progressively serious states of disease (healthy to mild to severe) escalates the importance of this study because there is not much research literature that considers ordinal outcomes in studies of this nature. The analysis can be segregated into 2 stages, univariate and multiprotein analysis. Approach of the univariate analysis was to implement continuation ratio model considering one protein at a time to pick those that exhibits potential ordinality. Penalized continuation ratio model using lasso regularization incorporated with bootstrapping proteins was performed as the next stage to identify protein combinationsthat perform well together. Compound results of the univariate and multi-protein analysis identified 20 most dominant proteins that have the capability to discriminate between the disease states in an ordinal manner satisfactorily. Keywords: Proteomic studies; Ordinal nature; Trend tests; Lasso regularization; Bootstrapping
蛋白质组学研究或蛋白质表达水平的研究随着技术的稳步提高和对影响人类的各种异常现象的了解而迅速发展。由于差异表达的蛋白质对整体细胞功能有影响,这提高了健康和患病状态之间的区分。鉴定主要蛋白为开发优化和靶向治疗方法提供了前瞻性的见解。这项研究包括分析一项早期研究的数据,该研究的主要目的是识别差异表达蛋白。三种逐渐严重的疾病状态(健康到轻度到严重)的存在提升了本研究的重要性,因为没有多少研究文献考虑这种性质的研究的顺序结果。分析可分为单变量分析和多蛋白分析2个阶段。单变量分析的方法是采用一次考虑一种蛋白质的延续比模型来选择那些表现出潜在规律性的蛋白质。利用lasso正则化结合自举蛋白的惩罚延续比模型,识别出协同性能良好的蛋白组合。单变量和多蛋白分析的复合结果令人满意地确定了20种最显性蛋白,它们具有以有序方式区分疾病状态的能力。关键词:蛋白质组学研究;自然顺序;趋势测试;套索正规化;引导
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引用次数: 0
Prosecuting Sexual Bribery in Sri Lanka: A study on Sexual Bribery and ‘Consent’ of the Victim 斯里兰卡的性贿赂起诉:性贿赂与受害人的“同意”研究
Kamini Rathnayake
The ability to prosecute Sexual Bribery (SB) in Sri Lanka (SL) is owed to statutory interpretation. However, SB of only public servants is considered bribery, whereas SB of private sector employees is considered as a sexual offence, thus entirely misrepresenting the gravity of the offence. While it is vital to address this discrepancy, it is equally important to classify SB under bribery and corruption. International review and research suggest the concept of ‘coerced consent’ to tackle the issues of prosecuting SB under laws for sexual offences. This study analyses that until SL amends its laws to expressly criminalise SB, in both public and private sectors, statutory interpretation of ‘coerced consent’ being incorporated into the legal system of SL seems a promising means to resolve this. Keywords: Sexual Bribery; Sextortion; Coerced Consent; Bribery and Corruption; Bribery Act
斯里兰卡(SL)的性贿赂(SB)起诉能力归功于法律解释。然而,只有公务员的SB才被视为贿赂,而私营机构雇员的SB则被视为性犯罪,从而完全歪曲了罪行的严重性。虽然解决这种差异至关重要,但将SB归类为贿赂和腐败也同样重要。国际审查和研究建议采用“强迫同意”的概念来解决根据性犯罪法律起诉SB的问题。本研究分析,在SL修改其法律明确将SB定为刑事犯罪之前,在公共和私营部门,将“强迫同意”的法定解释纳入SL的法律体系似乎是解决这一问题的有希望的手段。关键词:性贿赂;性勒索;强制许可;贿赂和腐败;贿赂行为
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引用次数: 0
Right to Information vs. Right to Protect Personal Data: A Guidance from the Decisions of the USA & the European Court of Justice. 信息权与个人数据保护权:美国和欧洲法院判决的指引。
R.W.D.N.K Rajapakse
Right to Information (RTI) and right to privacy (RTP) are two basic rights of people in a democratic country. Since personal data consists of a main element of privacy, the right not to disclose one's personal data to a third party is an essential part of the RTP. The citizens of Sri Lanka have had the right of obtaining information that is in the custody of public authorities since 2017 under the RTI Act. When deciding whether requested information will be disclosed or not, the public authority will have to consider the exceptions stipulated in the said Act, including the exception of privacy. However, with the enactment of the Personal Data Protection (PDP) Act in 2022 in Sri Lanka, it is crucial to consider the impact of the said Act on the RTI Act. Under the above context, this research explored the overlapping provisions of these two legislations and the decisions of the USA & the European Court of Justice in similar matters. This research study utilized the qualitative methodology where the researcher studied, analyzed, and synthesized a variety of materials gathered through primary and secondary sources to formulate a conclusion and come up with the study results. Finally, the research revealed that the PDP Act has put the additional liability on the public authorities when considering information requests under the RTI Act. Further, the research has identified some guidance from the decided cases of the USA & the European Court of Justice regarding overlapping provisions. Keywords: Right to information; right to privacy; personal data protection; public authorities.
知情权(RTI)和隐私权(RTP)是民主国家人民的两项基本权利。由于个人资料是私隐的一项主要内容,不向第三者披露个人资料的权利是保密协议的重要部分。自2017年以来,斯里兰卡公民有权根据《信息披露法》获取由公共当局保管的信息。在决定是否公开所要求的信息时,公共当局必须考虑该法案规定的例外情况,包括隐私例外情况。然而,随着斯里兰卡于2022年颁布的“个人数据保护(PDP)法案”,考虑该法案对RTI法案的影响至关重要。在上述背景下,本研究探讨了这两项立法的重叠规定以及美国和欧洲法院在类似事项上的判决。本研究采用定性研究方法,研究人员通过对第一手和二手资料收集的各种资料进行研究、分析和综合,得出结论,得出研究结果。最后,研究表明,PDP法使公共当局在考虑RTI法下的信息请求时承担了额外的责任。此外,研究还从美国和欧洲法院关于重叠条款的已判决案件中确定了一些指导。关键词:知情权;隐私权;个人资料保障;公共当局。
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引用次数: 0
To Leave or not to Leave: Attitudes of the Undergraduates towards Speaking in an Online ESL Classroom 离开还是不离开:大学生对在线ESL课堂发言的态度
Ashani Erandi Peiris
Covid 19 pandemic has affected all sectors in Sri Lanka including education. Online delivery mode was integrated into tertiary education in most of the universities to meet the new challenges. Facilitators of the English as a second language (ESL) classes use effective strategies and platforms to improve English language(L2) proficiency of the students. Improving speaking of the learners in such online ESL classes is challenging. ESL facilitator commonly experienced that some students leave the meeting when they are assigned to do a group activity or a speaking activity. Hence, it is vital to study the attitudes of the students towards speaking in an online class to help them improve speaking. A questionnaire was distributed among the first year, first and second semester students in a non-state university, and one hundred twenty-eight responses were collected. The results of the quantitative data were analysed using the percentages of the responses. As revealed, the main reason to avoid ESL lectures is irregular power cuts. On the other hand, the learners have not clearly identified the importance of L2 speaking. The majority use L2 only for oral exams/ assignments, and some prefer the recording. The decision to leave the online lecture when there are speaking activities is rooted mainly from psychological factors, and then secondly from linguistics factors. With proper guidance the students may change their attitudes towards L2 speaking, and their decision to leave the online lecture would be less. Keywords: Leave, online ESL lectures, Speaking, attitude, factors
2019冠状病毒病大流行影响了斯里兰卡的所有部门,包括教育。为了应对新的挑战,大多数高校都将在线教学模式融入到高等教育中。英语作为第二语言(ESL)课程的辅导员使用有效的策略和平台来提高学生的英语语言水平。在这样的在线ESL课程中提高学习者的口语能力是具有挑战性的。ESL引导者通常会遇到一些学生在被分配去做小组活动或演讲活动时离开会议的情况。因此,研究学生在网络课堂上对口语的态度,帮助他们提高口语水平是至关重要的。对一所非公立大学一、上、下学期的学生进行问卷调查,共收集回答者128人。定量数据的结果用回答的百分比来分析。据调查,不上课的主要原因是不定期停电。另一方面,学习者没有清楚地认识到第二语言口语的重要性。大多数人只在口试/作业中使用第二语言,有些人更喜欢录音。在有口语活动的情况下决定退出网络讲座,主要是出于心理因素,其次是语言学因素。在适当的指导下,学生可能会改变他们对第二语言口语的态度,他们离开在线课程的决定也会减少。关键词:请假,在线ESL讲座,口语,态度,因素
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引用次数: 0
Forging Ahead with Attitudinal Changes towards an Efficient Practice of Commercial Arbitration in Sri Lanka 对斯里兰卡商事仲裁有效实践的态度转变
K.G.T Dilhara
This study examines the question, of whether the pervading disappointment surrounding the practice of commercial arbitration in Sri Lanka, is due to the assimilation of court practices into commercial arbitration by the legal practitioners. Arbitration as an Alternative Dispute Resolution (ADR) method to judicial processes was introduced with the enactment of Arbitration Act No.11 of 1995 and hailed as an effective ADR for the resolution of commercial disputes. Participants were ten senior legal practitioners involved in both court practice as well as commercial arbitration for more than ten years in Sri Lanka. The study used a phenomenological research design to explore the perspectives of ten participants. It was hypothesised that the legal practitioners involved in both court and commercial arbitration practice would have a deeper understanding of the disappointments surrounding commercial arbitration practice and why commercial arbitration has not yielded the anticipated result of efficiency in dispute resolution over court practice. The results indicated that the same practitioners in court and arbitration, bring the same attitudes and skill set practised in courts into commercial arbitration. Thus, the emerging theme is to create an attitudinal shift in the participants of commercial arbitration for the speedy resolution of disputes in comparison to formal court practice. Keywords: Commercial arbitration; Alternative Dispute Resolution; speedy resolution of disputes; attitudinal shift.
本研究探讨的问题,是否普遍失望的做法商事仲裁在斯里兰卡,是由于法院的做法融入商事仲裁的法律从业人员。仲裁作为司法程序的一种替代性争议解决(ADR)方法是随着1995年《仲裁法》第11号的颁布而引入的,被誉为解决商业纠纷的有效ADR。与会者是十名在斯里兰卡从事法庭实践和商事仲裁工作十多年的资深法律从业人员。本研究采用现象学研究设计,对10名参与者的观点进行探讨。据推测,参与法院和商事仲裁实践的法律从业人员将对商事仲裁实践的令人失望之处有更深的理解,以及为什么商事仲裁没有产生比法院实践更有效的争议解决的预期结果。结果表明,法院和仲裁的相同从业人员将在法院实践的相同态度和技能组合带入商事仲裁。因此,新出现的主题是在商事仲裁参与者中创造一种态度转变,以便与正式的法庭实践相比,迅速解决争端。关键词:商事仲裁;替代性争议解决;迅速解决争端;态度的转变。
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引用次数: 0
Care of an Adult Patient who underwent a Series of Surgical Interventions for Psoas Abscess Complications with Haemophilia: Case Report 一例血友病并发腰肌脓肿接受一系列手术治疗的成人患者的护理:病例报告
H. Sepalika, M.D Dinuka, C. Dasanayaka
Psoas abscess is a collection of pus in the Iliopsoas muscle compartment. Haemophilia is an inherited congenital bleeding disorder with a lack of clotting factors. Patients with haemophilia commonly bleed in joints and muscles and experience delayed wound healing. Keywords: Psoas Abscess, Haemophilia, Vacuumdressing, Ileostomy,Sigmoidectomy
腰肌脓肿是髂腰肌间室脓液的集合。血友病是一种缺乏凝血因子的遗传性先天性出血性疾病。血友病患者通常关节和肌肉出血,伤口愈合延迟。关键词:腰肌脓肿,血友病,真空包扎,回肠造口术,乙状结肠切除术
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引用次数: 0
In Vitro Antibacterial Activity of Sulphur Nanoparticles as a Possible Application to Control Bacterial Blight Caused by Xanthomonas spp. in Anthurium 硫纳米颗粒的体外抑菌活性及其在红掌黄单胞菌病防治中的应用
S. Peiris, Gayani Malwattage, Raveena D Ratnayake, K. Seneviratne, C. N. Peiris
Anthurium blight is caused by Xanthomonas spp. which is regarded as the most threatening disease to the anthurium industry worldwide. Therefore, the current study was carried out to determine whether the application of sulphur nanoparticles (SNPs) is a possible solution for treating anthurium cultivars infected with Xanthomonas spp. The bacterium Xanthomonas was isolated using standard methods and a single bacterial colony was isolated using nutrient agar. The colonies were identified as Xanthomonas spp as they were gram-negative, motile rods due to the colony characters like yellow color because of the xanthin produced. The symptoms appeared in the pathogenicity test which was carried out by injecting purified Xanthomonas sp. into disease free anthurium plants confirmed the identification of the bacterial strain. Time-kill assay was conducted using Staphylococcus aureus, Escherichia coli and isolated Xanthomonas spp to investigate the behavior of SNPs. The results showed that suspension treated with 1g of SNPs for 30 minutes inhibited growth of Staphylococcus aureus colonies showing mean number of 7.92 CFU/ml compared to the control (mean number of colonies 9.09 CFU/ml ) treatment following 12 hours incubation. However, Escherichia coli, and isolated gram-negative rods (Xanthomonas spp) did not show positive influence for SNPs when compared to the control treatment. Therefore, further investigation is required to reach firm conclusions about this matter because the antimicrobial activity of SNPs varies depending on the type of target microorganisms, method and solvent used to dissolve SNPs. Keywords: Sulfur nanoparticles; Xanthomonas app; Anthurium; Bacterial blight
红掌疫病是由黄单胞菌(Xanthomonas spp.)引起的病害,是红掌产业中最具威胁性的病害。因此,本研究旨在探讨应用硫纳米颗粒(SNPs)治疗黄单胞菌感染红掌品种是否可行。采用标准方法分离黄单胞菌,并利用营养琼脂分离单个菌落。菌落为革兰氏阴性,菌落为黄单胞菌(Xanthomonas spp),菌落因产生黄质而呈黄色,具有运动杆状。将纯化的黄单胞菌注射到无病红掌植物中进行致病性试验,所出现的症状证实了该菌株的鉴定。采用金黄色葡萄球菌、大肠杆菌和分离的黄单胞菌进行时间杀伤实验,研究snp的行为。结果表明,1g SNPs处理30min后,培养12 h后,金黄色葡萄球菌的平均菌落数为7.92 CFU/ml,而对照组的平均菌落数为9.09 CFU/ml。然而,与对照处理相比,大肠杆菌和分离的革兰氏阴性棒(黄单胞菌)对snp没有积极影响。因此,由于SNPs的抗菌活性取决于目标微生物的类型、溶解SNPs的方法和溶剂,因此需要进一步的研究才能得出确切的结论。关键词:硫纳米颗粒;植物的应用;花烛属植物;白叶枯病
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引用次数: 0
A Fly in the Ointment; Undue Liability on E-commerce Platforms 美中不足;美中不足电子商务平台的不当责任
Krishanthi Rajaguru
Trade is now largely internet-centric, meaning internet is the medium through which most commercial transactions take place in today’s (information) economy. E-commerce has not only provided a greater market, but it also has changed the way businesses operate. This is indeed a platform economy. E-commerce is considered one of the main drivers of recent economic and social developments globally. In Sri Lanka e-commerce is emerging and in its infancy. The industry is currently expected to be operating within the margin of the law and selfregulated. In the absence of a separate law for e-commerce, e-commerce platforms (ecommerce marketplaces) meaning digital storefronts that connect sellers and customers to transact online, are exposed to a higher risk of being unreasonably penalized by applying the existing laws without mitigation. On the other hand, the platform users are left in a desperate situation with no remedy for harm caused. This article explores the responses of advanced jurisdictions such as China, the EU and the USA which are giant e-commerce markets, regarding platform liability. This study concludes that facilitating a business-enabled environment with holistic and innovative strategies that are aligned with social and economic status of the country with a businessfriendly legal landscape that matches the reality of the industry is imperative. Keywords: E-commerce, platform liability; platform law; policy consideration; law reforms.
贸易现在主要以互联网为中心,这意味着互联网是当今(信息)经济中大多数商业交易发生的媒介。电子商务不仅提供了更大的市场,而且还改变了企业的运作方式。这确实是一个平台经济。电子商务被认为是最近全球经济和社会发展的主要驱动力之一。在斯里兰卡,电子商务正在兴起并处于起步阶段。目前,预计该行业将在法律允许的范围内运作,并进行自我监管。在没有单独的电子商务法律的情况下,电子商务平台(电子商务市场),即连接卖家和客户进行在线交易的数字店面,面临着因不加缓解地适用现有法律而受到不合理处罚的更高风险。另一方面,平台用户处于绝望的境地,无法弥补造成的伤害。本文探讨了中国、欧盟和美国等大型电子商务市场的发达司法管辖区对平台责任的反应。该研究的结论是,必须通过与国家社会和经济地位相一致的整体和创新战略,以及与行业现实相匹配的商业友好型法律环境,促进有利于商业的环境。关键词:电子商务;平台责任;平台;政策的考虑;法律的改革。
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引用次数: 0
The Legal Debate on the Commercial Use of Personal Data - A Discussion of the Eu Gdpr Precedent 个人资料商业用途的法律辩论-欧盟Gdpr判例讨论
H. Senanayake
Although the adoption of the Personal Data Protection Act No. 9 of 2022 in Sri Lanka marked a significant milestone in the commercial use of personal data, the regulation of data use is often debated among international policymakers due to the inherent controversy of the subject. This is especially seen in the European Union (EU) which has a stringent data protection scheme. In light of this legal debate, the discussion in this study centres around the key concern of appropriate regulation and balancing between two competing rights, namely, the freedom to commercially utilise user data in the digital economy, and the protection of the right to privacy and protection from unlawful processing of personal data of the consumer/user. Such an academic conversation is engaged in by deliberating on the legal implications of commercial use of personal data. To this end, the essay will first examine the existing legal systems for commercially processing personal data with specific attention to the European Union (EU) General Data Protection Regulation (GDPR) of 2016 and the associated case law. Next, the essay will discuss three concerns on the present protectionist trajectory of the law, and its impact on the dual role of the law in the digital economy, i.e. as a facilitator of lawful commercial use of personal data and a guardian of privacy rights of data subjects. Thereafter, the essay will discuss three concerns on the present protectionist trajectory of the law, and its impact on the dual role of the law in the digital economy, i.e., as a facilitator of lawful commercial use of personal data and as a guardian of privacy rights of data subjects. The legal analysis is centralised on the EU personal data protection regime because it is a microcosm of development in general data protection law, which is widely accepted as a global persuasive precedent on the regulation of transnational commercial use of personal data. Keywords: Legal debate, personal data, Eu Gdpr Precedent.
尽管斯里兰卡通过了2022年第9号《个人数据保护法》,标志着个人数据商业使用的一个重要里程碑,但由于这一主题固有的争议,数据使用的监管经常在国际政策制定者之间进行辩论。这在拥有严格数据保护计划的欧盟(EU)尤为明显。鉴于这一法律辩论,本研究的讨论集中在适当监管和平衡两项相互竞争的权利的关键问题上,即在数字经济中商业利用用户数据的自由,以及保护隐私权和保护消费者/用户的个人数据不被非法处理。这种学术对话是通过审议商业使用个人数据的法律含义来进行的。为此,本文将首先研究现有的商业处理个人数据的法律制度,特别关注2016年欧盟(EU)通用数据保护条例(GDPR)和相关判例法。接下来,本文将讨论当前法律保护主义轨迹的三个问题,以及它对法律在数字经济中的双重角色的影响,即作为个人数据合法商业使用的促进者和数据主体隐私权的监护人。此后,本文将讨论目前法律保护主义轨迹的三个问题,以及它对法律在数字经济中的双重角色的影响,即作为个人数据合法商业使用的促进者和作为数据主体隐私权的监护人。法律分析集中在欧盟的个人数据保护制度上,因为它是一般数据保护法发展的一个缩影,它被广泛接受为监管跨国商业使用个人数据的一个有说服力的全球先例。关键词:法律辩论,个人数据,欧盟Gdpr先例。
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引用次数: 0
Effects of Coconut Water on Micropropagation of Caladium Bicolour Cv ’Thai Beauty’ 椰子水对‘泰美’钙双色Cv微繁的影响
Raveena D Ratnayake, S. Peiris, Gayani Malwattage, C. N. Peiris
Caladium bicolour (Aiton) Vent. is an attractive commercial ornamental plant in the horticulture industry which is popular as potted plants for homescaping and as garden plants for landscaping. Propagation of this attractive plant through micropropagation has more benefits than conventional propagation. The current study describes an in vitro multiplication of Caladium bicolour cv ‘Thai Beauty’ with coconut water as a supplement. Shoots derived from leaf explants were cultured on Murashige and Skoog (MS) medium supplemented with 0, 0.5, 1 and 1.5 mg/L (6- Benzylaminopurine) with and without 120 mL/L of coconut water. The results showed that coconut water (CW) with 0.5 and 1.5 mg/L BAP in the medium increased caladium shoot multiplication having an average of 6.67 and 7.75 shoots /shoot, respectively, with a large number of high-quality shoots. Coconut water alone in the medium also produced average of 3.42 shoots/ shoot. Hence, the current protocol provides a direct, cost-effective mass propagation method for large-scale commercial cultivation of caladium. Keywords: Caladium; Micropropagation; Coconut water; 6-benzylaminopurine
Caladium双色(Aiton)喷口是一种极具吸引力的商业观赏植物,在园艺业中作为盆栽植物和园林绿化植物很受欢迎。这种有吸引力的植物通过微繁繁殖比常规繁殖有更多的好处。目前的研究描述了用椰子水作为补充的Caladium双色cv ' Thai Beauty '的体外增殖。叶片外植体的芽在分别添加0、0.5、1和1.5 mg/L(6-苄基氨基嘌呤)和不添加120 mL/L椰子水的Murashige和Skoog (MS)培养基上培养。结果表明,添加0.5和1.5 mg/L BAP的椰子水能促进钙芽增殖,平均分别为6.67和7.75个芽/枝,并能产生大量优质芽。培养基中仅椰子水也平均产生3.42个芽/芽。因此,目前的协议提供了一种直接的,具有成本效益的大规模繁殖方法,用于大规模的商业化培养钾。关键词:贝母;微体繁殖;椰子汁;6-benzylaminopurine
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引用次数: 0
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