Digital tools, which offer superior accuracy compared to manual metrics, utilize radiological images for noninvasive data collection, providing a convenient means of obtaining skeletal data. The greater sciatic notch exhibits high sexual dimorphism and resistance to damage, and therefore serves as a viable tool for sex determination in poorly preserved skeletons, particularly in scenarios involving mass disasters, highly putrefied, and skeletonized cases. In addition to the width and depth of the greater sciatic notch, the length and angle of the posterior segment are highly specific parameters. This study aims to obtain accurate and standardized values for determining sex by measuring the posterior segment of the greater sciatic notch using postmortem computed tomography with a 16-slice multidetector row computed tomography scanner. The study revealed that except for depth and the distance between the ischial spine and deepest point, all measured variables of the greater sciatic notch were greater for women than men on both sides. The length of the posterior segment and the posterior angle on both sides showed the highest positive correlation and provided highly significant differences between males and females. These findings not only reinforce the utility of the greater sciatic notch in sex determination but also highlight the potential for more accurate and noninvasive methods in forensic investigations. Postmortem computed tomography accurately determines sex by measuring the posterior sciatic notch segment, achieving a 90.9% accuracy rate. This study, the first of its kind in India, utilized postmortem computed tomography ruler measurements for sex determination, specifically evaluating the role of the posterior segment dimensions of the greater sciatic notch in the North Indian population.
{"title":"PMCT-based sex determination using posterior segment of greater sciatic notch in North Indian population","authors":"Ruchi Kumari, Jay Narayan Pandit, Surya Kiran Panga, Swati Tyagi, Abhishek Yadav, Sudhir Kumar Gupta","doi":"10.1186/s41935-024-00394-1","DOIUrl":"https://doi.org/10.1186/s41935-024-00394-1","url":null,"abstract":"Digital tools, which offer superior accuracy compared to manual metrics, utilize radiological images for noninvasive data collection, providing a convenient means of obtaining skeletal data. The greater sciatic notch exhibits high sexual dimorphism and resistance to damage, and therefore serves as a viable tool for sex determination in poorly preserved skeletons, particularly in scenarios involving mass disasters, highly putrefied, and skeletonized cases. In addition to the width and depth of the greater sciatic notch, the length and angle of the posterior segment are highly specific parameters. This study aims to obtain accurate and standardized values for determining sex by measuring the posterior segment of the greater sciatic notch using postmortem computed tomography with a 16-slice multidetector row computed tomography scanner. The study revealed that except for depth and the distance between the ischial spine and deepest point, all measured variables of the greater sciatic notch were greater for women than men on both sides. The length of the posterior segment and the posterior angle on both sides showed the highest positive correlation and provided highly significant differences between males and females. These findings not only reinforce the utility of the greater sciatic notch in sex determination but also highlight the potential for more accurate and noninvasive methods in forensic investigations. Postmortem computed tomography accurately determines sex by measuring the posterior sciatic notch segment, achieving a 90.9% accuracy rate. This study, the first of its kind in India, utilized postmortem computed tomography ruler measurements for sex determination, specifically evaluating the role of the posterior segment dimensions of the greater sciatic notch in the North Indian population.","PeriodicalId":11507,"journal":{"name":"Egyptian journal of forensic sciences","volume":"8 1","pages":""},"PeriodicalIF":1.0,"publicationDate":"2024-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140940176","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-05-07DOI: 10.1186/s41935-024-00395-0
Mahrous A. Ibrahim, Ayman Fathi Elsharawy, Waleed E. Abo Baraka, Athar M. Khalifa, Metwally E Abdalla, Mohamed S. Nafie, Shaimaa A. Shehata, Gamal Abdelrahman Bakhaat
Cyclophosphamide (CP) is a chemotherapeutic and immunosuppressive agent that induces oxidative stress, causing lung tissue damage. The study aims to explore the antioxidant role of tert-butylhydroquinone (TBHQ) in ameliorating CP-induced lung toxicity exhibited as oxidative stress and programmed cell death. Thirty-two adult male rats were allocated randomly into four groups: group 1 (control), group 2 TBHQ 50 mg/kg orally for 14 days, and group 3 single dose of (200 mg/kg, CP, i.p.) on the 9th day. In group 4, TBHQ (50 mg/kg, orally) was provided for 14 days, and (200 mg/kg, CP, i.p.) was administrated on the 9th day. Rats’ body and lung weight were measured. Oxidative stress marker malondialdehyde (MDA) and pulmonary tissue enzymatic antioxidant levels were assessed: glutathione S transferase, catalase, superoxide dismutase, and glutathione peroxidase. Additionally, glutathione level was measured. Assessment of the levels of TNF-α, IL-1β, and IL-6 were done as well as histopathological and immunohistochemistry investigations. Molecular docking studies of the protein structures of p53-MDM2, IL-6, and IL-1β were performed. CP-intoxicated rats demonstrated a significant decline (CAT, GPx, SOD, GST, and GSH) levels and a significant increase in MDA levels. The proinflammatory parameters (TNF-α, IL-6, IL-1ß) were significantly elevated in group 3. The noted biochemical changes, accompanied by histopathological destruction, indicate CP-induced pulmonary tissue injury. TBHQ played a protective role by attenuating most of the aforementioned biochemical alterations and histopathological distortions in rats’ lungs. TBHQ might be utilized as a potential ameliorative agent to inhibit CP-induced pulmonary toxicity via TBHQ’s antioxidant and anti-inflammatory effects.
{"title":"Tert-butylhydroquinone prevents cyclophosphamide induce lung toxicity in rats via inhibiting oxidative stress and apoptosis: in vivo and in silico study","authors":"Mahrous A. Ibrahim, Ayman Fathi Elsharawy, Waleed E. Abo Baraka, Athar M. Khalifa, Metwally E Abdalla, Mohamed S. Nafie, Shaimaa A. Shehata, Gamal Abdelrahman Bakhaat","doi":"10.1186/s41935-024-00395-0","DOIUrl":"https://doi.org/10.1186/s41935-024-00395-0","url":null,"abstract":"Cyclophosphamide (CP) is a chemotherapeutic and immunosuppressive agent that induces oxidative stress, causing lung tissue damage. The study aims to explore the antioxidant role of tert-butylhydroquinone (TBHQ) in ameliorating CP-induced lung toxicity exhibited as oxidative stress and programmed cell death. Thirty-two adult male rats were allocated randomly into four groups: group 1 (control), group 2 TBHQ 50 mg/kg orally for 14 days, and group 3 single dose of (200 mg/kg, CP, i.p.) on the 9th day. In group 4, TBHQ (50 mg/kg, orally) was provided for 14 days, and (200 mg/kg, CP, i.p.) was administrated on the 9th day. Rats’ body and lung weight were measured. Oxidative stress marker malondialdehyde (MDA) and pulmonary tissue enzymatic antioxidant levels were assessed: glutathione S transferase, catalase, superoxide dismutase, and glutathione peroxidase. Additionally, glutathione level was measured. Assessment of the levels of TNF-α, IL-1β, and IL-6 were done as well as histopathological and immunohistochemistry investigations. Molecular docking studies of the protein structures of p53-MDM2, IL-6, and IL-1β were performed. CP-intoxicated rats demonstrated a significant decline (CAT, GPx, SOD, GST, and GSH) levels and a significant increase in MDA levels. The proinflammatory parameters (TNF-α, IL-6, IL-1ß) were significantly elevated in group 3. The noted biochemical changes, accompanied by histopathological destruction, indicate CP-induced pulmonary tissue injury. TBHQ played a protective role by attenuating most of the aforementioned biochemical alterations and histopathological distortions in rats’ lungs. TBHQ might be utilized as a potential ameliorative agent to inhibit CP-induced pulmonary toxicity via TBHQ’s antioxidant and anti-inflammatory effects.","PeriodicalId":11507,"journal":{"name":"Egyptian journal of forensic sciences","volume":"23 1","pages":""},"PeriodicalIF":1.0,"publicationDate":"2024-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140887916","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-05-06DOI: 10.1186/s41935-024-00392-3
Amanda Fonseca Leitzke, Danielle Tapia Bueno, Tais Poletti, Guilherme Kurz Maron, Bruno Vasconcellos Lopes, Eduarda Vitória Morais, Ana Paula de Oliveira Lopes Inacio, Caroline Ieque Silveira, Juliana Porciuncula da Silva, Daiane Dias, Netftali Lenin Villarreal Carreño, Claudio Martin Pereira de Pereira
Composites are materials that have multiple phases and have attracted much attention as they are able to improve physical and chemical properties of an isolated material. In this sense, these composites are commonly used as key components for two purposes: coloring and improving the operational properties; besides that, they have alternative synthesis routes that respect the principles of green chemistry. Thus, this reports the development of a new composite using natural products, indigo and kaolinite, for application in papilloscopy as a new nontoxic fingermark developer. The composite was obtained via green procedures and was characterized by spectroscopic and chromatographic techniques. Thus, to investigate the potential of the material as a fingermark developer, different techniques were applied such as depletion, aging, comparison with commercial powder, and development of latent fingermarks on different surfaces. Tests revealed that the composite presented good contrast and adhesion with the latent fingermarks, even after 15 days of deposition. This study presents a natural indigo/kaolinite composite powder that showed similar or higher efficiency when compared to the commercial fingerprint powder and was able to develop identifiable natural and sebaceous fingermarks. • A composite was formed from eco-friendly materials. • The composite formation follows the concepts of green chemistry and low cost. •The composite was applied as a latent fingermark developer.
{"title":"The effectiveness of natural indigo/kaolinite composite powder in the development of latent fingermarks","authors":"Amanda Fonseca Leitzke, Danielle Tapia Bueno, Tais Poletti, Guilherme Kurz Maron, Bruno Vasconcellos Lopes, Eduarda Vitória Morais, Ana Paula de Oliveira Lopes Inacio, Caroline Ieque Silveira, Juliana Porciuncula da Silva, Daiane Dias, Netftali Lenin Villarreal Carreño, Claudio Martin Pereira de Pereira","doi":"10.1186/s41935-024-00392-3","DOIUrl":"https://doi.org/10.1186/s41935-024-00392-3","url":null,"abstract":"Composites are materials that have multiple phases and have attracted much attention as they are able to improve physical and chemical properties of an isolated material. In this sense, these composites are commonly used as key components for two purposes: coloring and improving the operational properties; besides that, they have alternative synthesis routes that respect the principles of green chemistry. Thus, this reports the development of a new composite using natural products, indigo and kaolinite, for application in papilloscopy as a new nontoxic fingermark developer. The composite was obtained via green procedures and was characterized by spectroscopic and chromatographic techniques. Thus, to investigate the potential of the material as a fingermark developer, different techniques were applied such as depletion, aging, comparison with commercial powder, and development of latent fingermarks on different surfaces. Tests revealed that the composite presented good contrast and adhesion with the latent fingermarks, even after 15 days of deposition. This study presents a natural indigo/kaolinite composite powder that showed similar or higher efficiency when compared to the commercial fingerprint powder and was able to develop identifiable natural and sebaceous fingermarks. • A composite was formed from eco-friendly materials. • The composite formation follows the concepts of green chemistry and low cost. •The composite was applied as a latent fingermark developer.","PeriodicalId":11507,"journal":{"name":"Egyptian journal of forensic sciences","volume":"12 1","pages":""},"PeriodicalIF":1.0,"publicationDate":"2024-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140887917","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-04-19DOI: 10.1186/s41935-024-00391-4
Muhammed Emin Parlak, Bengü Berrak Özkul, Mucahit Oruç, Osman Celbiş
For over a century, anthropometric techniques, widely used by anthropologists and adopted by medical scientists, have been utilized for predicting stature and sex. This study, conducted on a Eastern Turkish sample, aims to predict sex and stature using foot measurements through linear methods and Artificial Neural Networks. Our research was conducted on 134 medical students, comprising 69 males and 65 females. Stature and weight were measured in a standard anatomical position in the Frankfurt Horizontal Plane with a stadiometer of 0.1 cm precision. Measurements of both feet's height, length, and breadth were taken using a Vernier caliper, osteometric board, and height scale. The data were analyzed using SPSS 26.00. It was observed that all foot dimensions in males were significantly larger than in females. Sex prediction using linear methods yielded an accuracy of 94.8%, with a stature estimation error of 4.15 cm. When employing Artificial Neural Networks, sex prediction accuracy increased to 97.8%, and the error in stature estimation was reduced to 4.07 cm. Our findings indicate that Artificial Neural Networks can work more effectively with such data. Using Artificial Neural Networks, the accuracy of sex prediction for both feet exceeded 95%. Additionally, the error in stature estimation was reduced compared to the formulas obtained through linear methods.
{"title":"Sex and stature estimation from anthropometric measurements of the foot: linear analyses and neural network approach on a Turkish sample","authors":"Muhammed Emin Parlak, Bengü Berrak Özkul, Mucahit Oruç, Osman Celbiş","doi":"10.1186/s41935-024-00391-4","DOIUrl":"https://doi.org/10.1186/s41935-024-00391-4","url":null,"abstract":"For over a century, anthropometric techniques, widely used by anthropologists and adopted by medical scientists, have been utilized for predicting stature and sex. This study, conducted on a Eastern Turkish sample, aims to predict sex and stature using foot measurements through linear methods and Artificial Neural Networks. Our research was conducted on 134 medical students, comprising 69 males and 65 females. Stature and weight were measured in a standard anatomical position in the Frankfurt Horizontal Plane with a stadiometer of 0.1 cm precision. Measurements of both feet's height, length, and breadth were taken using a Vernier caliper, osteometric board, and height scale. The data were analyzed using SPSS 26.00. It was observed that all foot dimensions in males were significantly larger than in females. Sex prediction using linear methods yielded an accuracy of 94.8%, with a stature estimation error of 4.15 cm. When employing Artificial Neural Networks, sex prediction accuracy increased to 97.8%, and the error in stature estimation was reduced to 4.07 cm. Our findings indicate that Artificial Neural Networks can work more effectively with such data. Using Artificial Neural Networks, the accuracy of sex prediction for both feet exceeded 95%. Additionally, the error in stature estimation was reduced compared to the formulas obtained through linear methods.","PeriodicalId":11507,"journal":{"name":"Egyptian journal of forensic sciences","volume":"44 1","pages":""},"PeriodicalIF":1.0,"publicationDate":"2024-04-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140629135","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-04-16DOI: 10.1186/s41935-024-00390-5
Othmane Essoubaiy, Bouchaïb Gazzaz, Hakima Yahia, Hicham EL Ossmani, Jalal Talbi, Brahim El Houate, Taoufiq Fechtali
The Chaouia - Ouradigha is a historical and ethno-geographic region of Morocco. Despite its historical relevance, this region has not received sufficient attention in terms of population genetic exploration. The present study aims to provide new data on the genetic background of the Chaouia-Ouardigha population, the Arabic-speaking North African population in the western center of Morocco, and to examine the relationship between this population and other reference populations based on our dataset as well as previously published population data. Genetic exploration will enable the reconstruction of past gene flows that have influenced the genetic makeup of this particular population. We utilized the Investigator® IDplex Plus PCR amplification kit (Qiagen, Germany) to establish the allelic frequencies and forensic statistical parameters of 15 autosomal Short Tandem Repeats (STRs) included in the kit in 153 random healthy unrelated individuals from the Chaouia-Ourdigha region. The Chaouia-Ouardigha population, which speaks Arabic, exhibits a high degree of genetic diversity with heterozygosity values ranging from 0.670 to 0.879. The loci with the highest allele frequencies, namely D7S820 and TPOX, have the lowest heterozygosity values (0.670 and 0.672, respectively), resulting in lower exclusion power (PE) and typical relatedness index (TPI) values (PE = 0.427 and TPI = 1.66 for TPOX; PE = 0.442 and TPI = 1.72 for D7S820). Phylogenetic analysis showed that the Arabic-speaking population of Chaouia-Ouardigha was found to be distinct from Sub-Saharan, East Asian, and Latin American populations. Our study provides insights into the genetic connections of the Chaouia Ouardigha population, highlighting how historical events, socio-cultural influences, and geographical proximity have contributed to shaping their genetic structure. It demonstrates the substantial impact of historical events and geographical closeness in fostering affinities between the Chaouia Ouardigha community and neighboring populations in North Africa, Southern Europe, and the Middle East. Additionally, the study has generated valuable data confirming the effectiveness of these 15 genetic markers for forensic applications across the broader population.
{"title":"Anthropogenetic study of the Arabic - speaking population of Chaouia Ouardigha (Morocco) based on autosomal STRs","authors":"Othmane Essoubaiy, Bouchaïb Gazzaz, Hakima Yahia, Hicham EL Ossmani, Jalal Talbi, Brahim El Houate, Taoufiq Fechtali","doi":"10.1186/s41935-024-00390-5","DOIUrl":"https://doi.org/10.1186/s41935-024-00390-5","url":null,"abstract":"The Chaouia - Ouradigha is a historical and ethno-geographic region of Morocco. Despite its historical relevance, this region has not received sufficient attention in terms of population genetic exploration. The present study aims to provide new data on the genetic background of the Chaouia-Ouardigha population, the Arabic-speaking North African population in the western center of Morocco, and to examine the relationship between this population and other reference populations based on our dataset as well as previously published population data. Genetic exploration will enable the reconstruction of past gene flows that have influenced the genetic makeup of this particular population. We utilized the Investigator® IDplex Plus PCR amplification kit (Qiagen, Germany) to establish the allelic frequencies and forensic statistical parameters of 15 autosomal Short Tandem Repeats (STRs) included in the kit in 153 random healthy unrelated individuals from the Chaouia-Ourdigha region. The Chaouia-Ouardigha population, which speaks Arabic, exhibits a high degree of genetic diversity with heterozygosity values ranging from 0.670 to 0.879. The loci with the highest allele frequencies, namely D7S820 and TPOX, have the lowest heterozygosity values (0.670 and 0.672, respectively), resulting in lower exclusion power (PE) and typical relatedness index (TPI) values (PE = 0.427 and TPI = 1.66 for TPOX; PE = 0.442 and TPI = 1.72 for D7S820). Phylogenetic analysis showed that the Arabic-speaking population of Chaouia-Ouardigha was found to be distinct from Sub-Saharan, East Asian, and Latin American populations. Our study provides insights into the genetic connections of the Chaouia Ouardigha population, highlighting how historical events, socio-cultural influences, and geographical proximity have contributed to shaping their genetic structure. It demonstrates the substantial impact of historical events and geographical closeness in fostering affinities between the Chaouia Ouardigha community and neighboring populations in North Africa, Southern Europe, and the Middle East. Additionally, the study has generated valuable data confirming the effectiveness of these 15 genetic markers for forensic applications across the broader population.","PeriodicalId":11507,"journal":{"name":"Egyptian journal of forensic sciences","volume":"48 1","pages":""},"PeriodicalIF":1.0,"publicationDate":"2024-04-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140586890","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Fingermarks are one of the oldest, reliable, and universally accepted evidence found on the crime scene. They can be used to link suspect with scene of crime and weapon of offence. Fingermarks are frequently used to investigate criminal cases and identify missing persons and criminals. Conventional methods such as cyanoacrylate fuming, iodine fuming, ninhydrin, silver nitrate, small particle reagent, and powder dusting are routinely used to detect and develop latent fingermarks on various surfaces of forensic importance. However, these methods suffer several limitations including poor contrast, low sensitivity, background interference, and low specificity. To overcome these limitations, nowadays, nanoparticles have gained importance in the development of latent fingermarks. In this review, we focus on the carbon dots (CD's) nanomaterial for the development of latent fingermarks. CD’s have superior fluorescence performance, color tuneability, and low synthesis cost and are non-toxic. The color and intensity of luminescence of CD's depend on its morphology and synthesis method. CD's can be used either in solid or solution form to develop latent fingermarks on the various porous and nonporous surfaces. CD’s are potentially a good candidate to develop latent fingermarks on wide range of porous and nonporous items of forensic importance. The fingerprints developed with CD’s show excellent contrast and resolution. Their small size, biocompatibility, facile and low-cost synthesis, and color tuneability can be successfully utilized to overcome the limitations of the conventional methods.
指痕是在犯罪现场发现的最古老、可靠和普遍接受的证据之一。它们可以用来将嫌疑人与犯罪现场和犯罪武器联系起来。指痕经常被用于调查刑事案件、识别失踪人员和罪犯。传统方法，如氰基丙烯酸酯熏蒸法、碘熏蒸法、茚三酮法、硝酸银法、小颗粒试剂法和粉末撒粉法，通常用于检测和显现各种重要法证表面上的潜在指痕。然而，这些方法存在一些局限性，包括对比度差、灵敏度低、背景干扰和特异性低。为了克服这些局限性，如今纳米粒子在潜伏指痕的发展中变得越来越重要。在本综述中，我们将重点介绍用于潜伏指痕开发的碳点（CD）纳米材料。碳点具有优异的荧光性能、颜色可调节性、低合成成本和无毒性。CD 的颜色和发光强度取决于其形态和合成方法。CD 可以固体或溶液形式用于在各种多孔和无孔表面形成潜指印。CD 有可能成为在各种多孔和无孔法医物品上提取潜在指印的理想选择。用 CD 制作的指纹具有极佳的对比度和分辨率。它们具有体积小、生物相容性好、合成简便且成本低廉、颜色可调等优点，可以成功地克服传统方法的局限性。
{"title":"A comprehensive review on the detection of latent fingermarks using carbon dots","authors":"Aseem Grover, Laxmi Devi, Jyotirmoy Maity, Gurvinder Singh Bumbrah, Anirban Das","doi":"10.1186/s41935-024-00388-z","DOIUrl":"https://doi.org/10.1186/s41935-024-00388-z","url":null,"abstract":"Fingermarks are one of the oldest, reliable, and universally accepted evidence found on the crime scene. They can be used to link suspect with scene of crime and weapon of offence. Fingermarks are frequently used to investigate criminal cases and identify missing persons and criminals. Conventional methods such as cyanoacrylate fuming, iodine fuming, ninhydrin, silver nitrate, small particle reagent, and powder dusting are routinely used to detect and develop latent fingermarks on various surfaces of forensic importance. However, these methods suffer several limitations including poor contrast, low sensitivity, background interference, and low specificity. To overcome these limitations, nowadays, nanoparticles have gained importance in the development of latent fingermarks. In this review, we focus on the carbon dots (CD's) nanomaterial for the development of latent fingermarks. CD’s have superior fluorescence performance, color tuneability, and low synthesis cost and are non-toxic. The color and intensity of luminescence of CD's depend on its morphology and synthesis method. CD's can be used either in solid or solution form to develop latent fingermarks on the various porous and nonporous surfaces. CD’s are potentially a good candidate to develop latent fingermarks on wide range of porous and nonporous items of forensic importance. The fingerprints developed with CD’s show excellent contrast and resolution. Their small size, biocompatibility, facile and low-cost synthesis, and color tuneability can be successfully utilized to overcome the limitations of the conventional methods.","PeriodicalId":11507,"journal":{"name":"Egyptian journal of forensic sciences","volume":"51 1","pages":""},"PeriodicalIF":1.0,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140586800","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-03-15DOI: 10.1186/s41935-024-00389-y
Lina Bhoyar, Palash Mehar, Krishnadutt Chavali
DNA (deoxy-ribonucleic acid) is a fundamental molecule housing genetic information crucial for forensic casework. However, its integrity is compromised over time due to degradation, affecting living and deceased organisms. Understanding the factors and mechanisms of DNA degradation is vital across scientific disciplines. DNA degradation is a dynamic process influenced by factors like temperature, humidity, and ultraviolet radiation. The post-mortem interval affects organisms differently, and mechanisms such as hydrolysis, oxidation, and depurination impact DNA structural integrity. In forensic casework, DNA degradation poses challenges because degraded DNA samples can be difficult to analyze. Despite these challenges, DNA degradation has become an invaluable asset in forensic science. Fragmented DNA aids in historical identification and archaeological investigations. Additionally, DNA degradation helps estimate the time since death, assisting investigators in criminal timelines. Forensic experts use degradation patterns to deduce environmental conditions affecting a body, aiding crime scene reconstruction. In criminal investigations, advancements in DNA recovery, like next-generation sequencing, enable the analysis of severely degraded samples, enhancing the identification of suspects and victims. DNA degradation, despite its challenges, is a potent tool in forensic science. Understanding the factors and mechanisms influencing DNA degradation is essential for its effective utilization in criminal casework. With ongoing advancements in forensic techniques and technologies, the reliability and utility of degraded DNA analysis are steadily increasing, helping to bring resolution to complex criminal cases and uncovering hidden clues in forensic investigations.
DNA（脱氧核糖核酸）是一种基本分子，包含对法医办案至关重要的遗传信息。然而，随着时间的推移，DNA 的完整性会因降解而受到破坏，影响到活体和已死亡的生物体。了解 DNA 降解的因素和机制对各科学学科都至关重要。DNA 降解是一个受温度、湿度和紫外线辐射等因素影响的动态过程。死后间隔期对生物的影响各不相同，水解、氧化和去质化等机制都会影响 DNA 结构的完整性。在法医办案工作中，DNA 降解带来了挑战，因为降解的 DNA 样本可能难以分析。尽管存在这些挑战，DNA 降解已成为法医学的宝贵财富。支离破碎的 DNA 有助于历史鉴定和考古调查。此外，DNA 降解还有助于估算死亡时间，帮助调查人员确定犯罪时间表。法医专家利用降解模式来推断影响尸体的环境条件，从而帮助重建犯罪现场。在刑事调查中，DNA 恢复技术的进步（如下一代测序技术）使得对严重降解样本的分析成为可能，从而加强了对嫌疑人和受害者的识别。DNA 降解尽管具有挑战性，但却是法医学的有力工具。了解影响 DNA 降解的因素和机制对于在刑事案件工作中有效利用 DNA 至关重要。随着法医技术和科技的不断进步，降解 DNA 分析的可靠性和实用性也在稳步提高，有助于解决复杂的刑事案件，发现法医调查中隐藏的线索。
{"title":"An overview of DNA degradation and its implications in forensic caseworks","authors":"Lina Bhoyar, Palash Mehar, Krishnadutt Chavali","doi":"10.1186/s41935-024-00389-y","DOIUrl":"https://doi.org/10.1186/s41935-024-00389-y","url":null,"abstract":"DNA (deoxy-ribonucleic acid) is a fundamental molecule housing genetic information crucial for forensic casework. However, its integrity is compromised over time due to degradation, affecting living and deceased organisms. Understanding the factors and mechanisms of DNA degradation is vital across scientific disciplines. DNA degradation is a dynamic process influenced by factors like temperature, humidity, and ultraviolet radiation. The post-mortem interval affects organisms differently, and mechanisms such as hydrolysis, oxidation, and depurination impact DNA structural integrity. In forensic casework, DNA degradation poses challenges because degraded DNA samples can be difficult to analyze. Despite these challenges, DNA degradation has become an invaluable asset in forensic science. Fragmented DNA aids in historical identification and archaeological investigations. Additionally, DNA degradation helps estimate the time since death, assisting investigators in criminal timelines. Forensic experts use degradation patterns to deduce environmental conditions affecting a body, aiding crime scene reconstruction. In criminal investigations, advancements in DNA recovery, like next-generation sequencing, enable the analysis of severely degraded samples, enhancing the identification of suspects and victims. DNA degradation, despite its challenges, is a potent tool in forensic science. Understanding the factors and mechanisms influencing DNA degradation is essential for its effective utilization in criminal casework. With ongoing advancements in forensic techniques and technologies, the reliability and utility of degraded DNA analysis are steadily increasing, helping to bring resolution to complex criminal cases and uncovering hidden clues in forensic investigations.","PeriodicalId":11507,"journal":{"name":"Egyptian journal of forensic sciences","volume":"116 1","pages":""},"PeriodicalIF":1.0,"publicationDate":"2024-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140149285","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-02-13DOI: 10.1186/s41935-024-00387-0
Izzah Syahira Omar, Md Yusop Nur Hafiza, Zainuddin Zafarina, Mohd Nafi Siti Norasikin, Mohd Isa Seoparjoo Azmel, Mohamed Yusoff Shafini, Hanis Z. A. NurWaliyuddin
Short Tandem Repeats (STRs) are segments of DNA composed of a short sequence of nucleotides that repeat consecutively. These repeating sequences exhibit distinct lengths and nucleotide sequences among individuals, showcasing high variability and uniqueness. The STR profile remains consistent across all cells in an individual’s body. Nonetheless, changes in the STR profile have been documented in cancerous tissues. This scoping review aimed to investigate the occurrence and pattern of forensic STR markers alterations in cancerous tissues. We conducted a scoping review of the English-language publications published between 2002 and 2022 in the PubMed, Science Direct, and Scopus databases and a manual search of reference lists from reviewed papers. The review was carried out in compliance with the Preferred Reporting Items for Systematic reviews and Meta-Analyses extension for Scoping Reviews. Our search resulted in a total of 1,065 articles associating forensic STR studies with cancerous tissues. A total of 18 of these studies met our inclusion criteria. The D18S51 marker was most often found to be altered when associated with cancers such as breast, colorectal, gastric, gynaecology, and lung cancers. Following with that, FGA, VWA, D19S433, and D13S317 markers could as well be seen to have allelic alteration in cancerous tissues. Four other STR markers (TPOX, D7S820, D2S1338, and Penta D) could be potentially represented as stable STR markers in cancerous tissues. According to our review, colorectal cancer tissue has the highest level of genomic instability compared to that of other cancer types. In summary, the genetic instability caused by faulty DNA mismatch repair processes in human carcinomas can pose challenges for forensic genotyping and DNA profile matching.
短串联重复序列（STR）是由连续重复的核苷酸短序列组成的 DNA 片段。这些重复序列在个体间表现出不同的长度和核苷酸序列，具有很高的变异性和独特性。个体体内所有细胞的 STR 特征保持一致。然而，在癌症组织中也有 STR 图谱变化的记录。本范围综述旨在调查癌症组织中法医 STR 标记改变的发生情况和模式。我们对 PubMed、Science Direct 和 Scopus 数据库中 2002 年至 2022 年间发表的英文出版物进行了范围界定综述，并对综述论文的参考文献列表进行了人工检索。该综述是根据《系统综述和元分析首选报告项目》（Preferred Reporting Items for Systematic Review and Meta-Analyses）扩展到《范围界定综述》（Scoping Reviews）的要求进行的。我们的检索结果显示，共有 1,065 篇文章将法医 STR 研究与癌症组织相关联。其中共有 18 项研究符合我们的纳入标准。在与乳腺癌、结直肠癌、胃癌、妇科癌和肺癌等癌症相关的研究中，D18S51 标记最常发生变化。此外，FGA、VWA、D19S433 和 D13S317 标记在癌症组织中也有等位基因改变。另外四个 STR 标记（TPOX、D7S820、D2S1338 和 Penta D）有可能成为癌症组织中稳定的 STR 标记。根据我们的研究，与其他癌症类型相比，结直肠癌组织的基因组不稳定性水平最高。总之，人类癌症中 DNA 错配修复过程的缺陷所导致的基因不稳定性会给法医基因分型和 DNA 图谱比对带来挑战。
{"title":"Forensic short tandem repeat markers alteration in cancerous tissues: a scoping review","authors":"Izzah Syahira Omar, Md Yusop Nur Hafiza, Zainuddin Zafarina, Mohd Nafi Siti Norasikin, Mohd Isa Seoparjoo Azmel, Mohamed Yusoff Shafini, Hanis Z. A. NurWaliyuddin","doi":"10.1186/s41935-024-00387-0","DOIUrl":"https://doi.org/10.1186/s41935-024-00387-0","url":null,"abstract":"Short Tandem Repeats (STRs) are segments of DNA composed of a short sequence of nucleotides that repeat consecutively. These repeating sequences exhibit distinct lengths and nucleotide sequences among individuals, showcasing high variability and uniqueness. The STR profile remains consistent across all cells in an individual’s body. Nonetheless, changes in the STR profile have been documented in cancerous tissues. This scoping review aimed to investigate the occurrence and pattern of forensic STR markers alterations in cancerous tissues. We conducted a scoping review of the English-language publications published between 2002 and 2022 in the PubMed, Science Direct, and Scopus databases and a manual search of reference lists from reviewed papers. The review was carried out in compliance with the Preferred Reporting Items for Systematic reviews and Meta-Analyses extension for Scoping Reviews. Our search resulted in a total of 1,065 articles associating forensic STR studies with cancerous tissues. A total of 18 of these studies met our inclusion criteria. The D18S51 marker was most often found to be altered when associated with cancers such as breast, colorectal, gastric, gynaecology, and lung cancers. Following with that, FGA, VWA, D19S433, and D13S317 markers could as well be seen to have allelic alteration in cancerous tissues. Four other STR markers (TPOX, D7S820, D2S1338, and Penta D) could be potentially represented as stable STR markers in cancerous tissues. According to our review, colorectal cancer tissue has the highest level of genomic instability compared to that of other cancer types. In summary, the genetic instability caused by faulty DNA mismatch repair processes in human carcinomas can pose challenges for forensic genotyping and DNA profile matching.","PeriodicalId":11507,"journal":{"name":"Egyptian journal of forensic sciences","volume":"99 1","pages":""},"PeriodicalIF":1.0,"publicationDate":"2024-02-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139754116","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-02-03DOI: 10.1186/s41935-024-00386-1
Walid Mahmoud Khalilia, Simon Ricard, Abd Al Latif Rabaia, Mathieu Arès, Frank Crispino
Crime scene investigation (CSI) in general means the standard procedures and techniques used for processing and reconstructing of scene of crime. In Palestine, the competent authorities delegated by law to carry out the task of research and investigation of crimes and inspection at the crime scene are the officers granted by law the status of the judicial police. CSIs face numerous challenges that affect every worker involved. These challenges arise from legal, administrative, security, and technical aspects. This study aimed to point out the challenges faced by field police personnel during CSI in Palestine. To achieve the aims of this study, a validated and reliable questionnaire was developed. The study sample consisted of 354 crime scene investigators and officers affiliated with the Palestinian Civil Police (PCP) across all governorates of the West Bank. In addition to the training of CSI officers, and shortage of equipment, the findings of this study indicate that there are many challenges amplified by the Israeli occupation facing PCP officers concerning crime scene management and technical procedures during CSIs such as the collection, transportation, and storage of forensic evidence. Overlapping responsibilities and difficulties in coordinating specialized agencies working at the crime scene are also factors that should be better studied. This study invites decision-makers within the Palestinian police agencies to prioritize efforts to address the significant challenges encountered by workers at crime scenes. They should give special attention on enhancing training programs and providing the necessary tools and devices for effective crime scene work. It also recommends Palestinian representatives at international stage to highlight the obstacles facing workers at crime scenes due to the occupation.
{"title":"Challenges facing Palestinian crime scene investigators","authors":"Walid Mahmoud Khalilia, Simon Ricard, Abd Al Latif Rabaia, Mathieu Arès, Frank Crispino","doi":"10.1186/s41935-024-00386-1","DOIUrl":"https://doi.org/10.1186/s41935-024-00386-1","url":null,"abstract":"Crime scene investigation (CSI) in general means the standard procedures and techniques used for processing and reconstructing of scene of crime. In Palestine, the competent authorities delegated by law to carry out the task of research and investigation of crimes and inspection at the crime scene are the officers granted by law the status of the judicial police. CSIs face numerous challenges that affect every worker involved. These challenges arise from legal, administrative, security, and technical aspects. This study aimed to point out the challenges faced by field police personnel during CSI in Palestine. To achieve the aims of this study, a validated and reliable questionnaire was developed. The study sample consisted of 354 crime scene investigators and officers affiliated with the Palestinian Civil Police (PCP) across all governorates of the West Bank. In addition to the training of CSI officers, and shortage of equipment, the findings of this study indicate that there are many challenges amplified by the Israeli occupation facing PCP officers concerning crime scene management and technical procedures during CSIs such as the collection, transportation, and storage of forensic evidence. Overlapping responsibilities and difficulties in coordinating specialized agencies working at the crime scene are also factors that should be better studied. This study invites decision-makers within the Palestinian police agencies to prioritize efforts to address the significant challenges encountered by workers at crime scenes. They should give special attention on enhancing training programs and providing the necessary tools and devices for effective crime scene work. It also recommends Palestinian representatives at international stage to highlight the obstacles facing workers at crime scenes due to the occupation.","PeriodicalId":11507,"journal":{"name":"Egyptian journal of forensic sciences","volume":"185 1","pages":""},"PeriodicalIF":1.0,"publicationDate":"2024-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139664426","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Proper investigation of digital evidence is of prime significance in cyber investigations. From the collection of evidence, its preservation, and its analysis, it is important to maintain its integrity in the legal system due to the involvement of different stakeholders like law enforcement agencies, digital analysts, and the judiciary. This review paper focuses on how blockchain technology can be used to collect evidence efficiently. In the present scenario, the manual logs of the chain of custody are used to ensure that the evidence remains confidential and transparent. It is accompanied by filling out the application forms and maintaining logs within the organization handling the evidence. Hence, it is important to ensure the validity, integrity, and verifiability of evidence as it moves through different hierarchical levels. There are certain issues associated with the current chain of custody, such as evidence loss, theft, tampering, and, even worse, evidence manipulation inside the system. To avoid this situation and to make the process coherent, this review paper aims to highlight the potential use of blockchain technology to preserve chain of custody. Although this scientific technology is mainly used to run cryptocurrencies, with careful consideration and application, this could play a key role in supporting and managing the chain of custody. It is a distributed database that keeps track of blocks. These blocks are collection of entries that keep growing continually and are secured from editing and manipulation by retaining the hash of the previous block in the chain. This is a decentralized technology that is not easily compromised in terms of security and therefore has the potential to solve our problem area. A future research agenda needs to be established, which lays the solid foundation for further studies on this evident emerging area.
{"title":"Potential applicability of blockchain technology in the maintenance of chain of custody in forensic casework","authors":"Harsh Patil, Ravshish Kaur Kohli, Sorabh Puri, Pooja Puri","doi":"10.1186/s41935-023-00383-w","DOIUrl":"https://doi.org/10.1186/s41935-023-00383-w","url":null,"abstract":"Proper investigation of digital evidence is of prime significance in cyber investigations. From the collection of evidence, its preservation, and its analysis, it is important to maintain its integrity in the legal system due to the involvement of different stakeholders like law enforcement agencies, digital analysts, and the judiciary. This review paper focuses on how blockchain technology can be used to collect evidence efficiently. In the present scenario, the manual logs of the chain of custody are used to ensure that the evidence remains confidential and transparent. It is accompanied by filling out the application forms and maintaining logs within the organization handling the evidence. Hence, it is important to ensure the validity, integrity, and verifiability of evidence as it moves through different hierarchical levels. There are certain issues associated with the current chain of custody, such as evidence loss, theft, tampering, and, even worse, evidence manipulation inside the system. To avoid this situation and to make the process coherent, this review paper aims to highlight the potential use of blockchain technology to preserve chain of custody. Although this scientific technology is mainly used to run cryptocurrencies, with careful consideration and application, this could play a key role in supporting and managing the chain of custody. It is a distributed database that keeps track of blocks. These blocks are collection of entries that keep growing continually and are secured from editing and manipulation by retaining the hash of the previous block in the chain. This is a decentralized technology that is not easily compromised in terms of security and therefore has the potential to solve our problem area. A future research agenda needs to be established, which lays the solid foundation for further studies on this evident emerging area.","PeriodicalId":11507,"journal":{"name":"Egyptian journal of forensic sciences","volume":"23 1","pages":""},"PeriodicalIF":1.0,"publicationDate":"2024-01-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139585155","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
京公网安备 11010802042870号
京ICP备2023020795号-1
扫码关注我们
求助内容：
应助结果提醒方式：