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Valorisation of the Effects of Bioactive Compounds of the Ethanolic Extract of Ramalina Farinacea (Ramalinaceae) on the Development, Eating and Pupation Behavior of Drosophila Melanogaster (Diptera: Drosophilidae) Ramalina Farinacea (Ramalinaceae)乙醇提取物生物活性成分对黑腹果蝇(Drosophila Melanogaster)发育、摄食及化蛹行为的影响
Pub Date : 2021-12-31 DOI: 10.35691/jbm.1202.0208
F. Saadane, Nour El Iméne Boublata, Sarra Habbachi, A. Bouzar, W. Habbachi, A. Slimani, A. Tahraoui
Plants are rich in bioactive chemical secondary metabolites and have proven insecticidal activity by killing or repelling insects. In this work, we aim to evaluate the direct and delayed effects of ethanolic plant extracts on the vinegar fly (Drosophila melanogaster). The treatment was performed by ingestion on second instar larvae (L2) to evaluate the impact of the ethanolic extract on development for 15 days and subsequently on the feeding behavior of the larvae. The results of this study indicate a slowing down of pupal growth until the adult stage, at the three concentrations (0.25 µg/ml, 0.5 µg/ml, 1.5 µg/ml, 2 µg/ml) used. The results also showed that after three days of treatment, third instar D. melanogster larvae lost the ability to detect the odors of their nutrient environments. Other numbers of larvae (34 %) do not make a choice in the different tests performed. This study indicates that the ethanolic extract of Ramalina farinacea has a neurotoxic property our results confirmed the presence of toxic secondary metabolites which have bioinsecticidal activities in this extract.
植物富含生物活性化学次生代谢物,已被证明具有杀虫或驱虫的杀虫活性。在这项工作中,我们旨在评估乙醇植物提取物对醋蝇(Drosophila melanogaster)的直接和延迟效应。通过对2龄幼虫(L2)进行摄食处理,观察15 d后乙醇提取物对幼虫发育和摄食行为的影响。本研究结果表明,在三种浓度(0.25µg/ml, 0.5µg/ml, 1.5µg/ml, 2µg/ml)下,蛹的生长直到成虫阶段都有所减缓。结果还表明,经过三天的处理,三龄黑腹夜蛾幼虫失去了检测其营养环境气味的能力。其他数量的幼虫(34%)在进行的不同测试中没有做出选择。本研究表明,拉玛丽娜乙醇提取物具有神经毒性,结果证实了该提取物中存在具有生物杀虫活性的毒性次生代谢物。
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引用次数: 0
Estimation of Genetic Diversity among Canola Accessions using Simple Sequence Repeat Markers 利用简单序列重复标记估计油菜种质遗传多样性
Pub Date : 2021-10-28 DOI: 10.35691/jbm.1202.0205
Jaleel Ahmad, M. Baber, Wajid Nazeer, Sana Hamdullah, Aleena Ahmad Somroo
Genetic studies through molecular markers proved important to find out the genetic diversity of canola. In this study, 50 lines of canola were used to find the polymorphism using 15 SSR primers and investigated the genetic diversity, PIC values, frequency-based genetic distance, and allelic frequencies. Mean gene diversity, frequency-based genetic distance, and PIC values were 0.8777, 0.233 and 0.8666, respectively for the canola lines. A good range of genetic diversity was found among studied canola lines with value 85.91% polymorphism. Maximum and minimum genetic distances among 50 lines were 1 and 0.26, respectively. Accessions ACC-26068, ACC-24241, ACC-24244, ACC-24233, ACC-24423 and ACC-24224 have maximum genetic distance. Accessions ACC-24879 and ACC-24169 had minimum genetic distance i.e., 0.26. Dendrogram based on genetic distances showed four main clusters that were further dividing into several sub-clusters. The primers utilized in the present study, were valuable to identify different accessions of canola to find the variability present. This variability will be helpful to initiate the breeding program with their molecular genetic basis.
利用分子标记技术进行遗传研究对了解油菜的遗传多样性具有重要意义。以50份油菜籽为材料,利用15条SSR引物进行多态性分析,并对其遗传多样性、PIC值、基于频率的遗传距离和等位基因频率进行了分析。油菜系的平均基因多样性、频率遗传距离和PIC值分别为0.8777、0.233和0.8666。油菜系间遗传多样性较好,多态性值为85.91%。50个品系间的最大遗传距离为1,最小遗传距离为0.26。ACC-26068、ACC-24241、ACC-24244、ACC-24233、ACC-24423和ACC-24224遗传距离最大。ACC-24879和ACC-24169的遗传距离最小,为0.26。基于遗传距离的树状图显示出4个主要簇,并进一步划分为几个亚簇。在本研究中使用的引物,是有价值的,以确定不同的油菜品种的变异存在。这种变异将有助于利用其分子遗传基础启动育种计划。
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引用次数: 1
Plastic Biodegradation through Insects and their Symbionts Microbes: A Review 昆虫及其共生微生物降解塑料的研究进展
Pub Date : 2021-10-28 DOI: 10.35691/jbm.1202.0206
Huda Bilal, H. Raza, Haseena Bibi, Tehmina Bibi
Plastic waste has recently been identified as one of the most serious environmental issues, affecting all life forms, natural habitats, and the economy, and is one of the most serious global environmental problems, second only to climate change. Seeking alternative environmentally sustainable options, such as biodegradation instead of conventional disposal, is critical in the face of this challenge. However, there is currently a lack of information about the mechanisms and efficacy of plastic biodegradation. From this perspective, this study aims to illustrate the negative environmental impacts of the plastic waste. It also addresses the role of insects and gut microbiota in the degradation of plastics, emphasizing the important role they will play in the future.
塑料垃圾最近被确定为最严重的环境问题之一,影响到所有的生命形式、自然栖息地和经济,是仅次于气候变化的最严重的全球环境问题之一。面对这一挑战,寻求其他环境可持续的选择,如生物降解代替传统处置,是至关重要的。然而,目前关于塑料生物降解的机制和功效的信息还很缺乏。从这个角度出发,本研究旨在说明塑料垃圾对环境的负面影响。它还讨论了昆虫和肠道微生物群在塑料降解中的作用,强调了它们在未来将发挥的重要作用。
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引用次数: 2
Prevalence and Multidrug Resistance Pattern of E. Coli among Urinary Tract Infection Patients in Tertiary Care Hospital of Multan 木尔坦三级医院尿路感染患者大肠杆菌的流行及多重耐药模式
Pub Date : 2021-10-28 DOI: 10.35691/jbm.1202.0207
Hubza Ruatt Khan, Mehvish Javeed, Asghar Javed, Nisma Farooq
Urinary Tract Infection is alarming problem worldwide due to the intensity of antimicrobial resistance. Escherichia coli is the most predominant organism in UTI. This study was planned to evaluate demographic parameters, the prevalence of E. coli, and antimicrobial resistance patterns among E. coli isolates from UTI patients in Nishtar Hospital of Multan from January to June 2018. A total of 350 mid-stream urine samples were collected from different patients having age group from 25 to 60 years and processed by standard laboratory procedures. Out of 350 samples, 100 samples were observed as critical bacteremia. Escherichia coli and Klebsiella pneumonia were the most persistent (47 % and 19 % individually) among the Gram-negative pathogens followed by S. aureus (14 %), Enterobacter spp. (11 %) and Candida (9 %) respectively. The incidence of UTI was found higher in 25-35 age groups. The prevalence of UTI with E. coli as an infectious agent was 72 % in females, and 28 % in males. The prevalence of E. coli was more in rural areas (70 %) than in urban areas (26 %). The antimicrobial testing against E. coli showed the highest resistance to amoxicillin (65.9 %) and ciprofloxacin (38.2 %), whereas highly sensitive rate observed against Fosfomycin (FOS) (95.7 %), Gentamicin (GEN) (89.3 %), and Nitrofurantoin (NIT) (85 %) respectively. The increased resistance against ampicillin and ciprofloxacin was observed in Multan have a great emerging problem so there is a need for effective prevention strategies for the E. coli drug resistance and successful surveillance required to be improved.
由于抗菌素耐药性的强度,尿路感染是世界范围内令人担忧的问题。大肠杆菌是尿路感染中最主要的微生物。本研究计划评估2018年1月至6月木尔坦Nishtar医院尿路感染患者分离出的大肠杆菌的人口统计学参数、大肠杆菌的流行率和耐药性模式。从年龄在25岁到60岁的不同患者中收集了350份中游尿液样本,并按标准实验室程序进行了处理。在350个样本中,有100个样本被观察到为临界菌血症。在革兰氏阴性病原菌中,大肠杆菌和肺炎克雷伯菌的持久性最强(分别为47%和19%),其次是金黄色葡萄球菌(14%)、肠杆菌(11%)和念珠菌(9%)。25-35岁年龄组的尿路感染发病率较高。以大肠杆菌为感染源的尿路感染的流行率在女性中为72%,在男性中为28%。大肠杆菌的流行率在农村地区(70%)高于城市地区(26%)。大肠杆菌对阿莫西林(65.9%)和环丙沙星(38.2%)的耐药率最高,对磷霉素(95.7%)、庆大霉素(89.3%)和呋喃妥英(85%)的耐药率最高。木尔坦地区对氨苄西林和环丙沙星的耐药性增加是一个突出的问题,因此需要制定有效的预防策略,并需要改进成功的监测。
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引用次数: 0
Salinity Stress Mitigation by Foliar Feeding of Salicylic Acid on Maize (Zea Mays L.) grown under Hydroponic Culture 水培玉米叶面补饲水杨酸缓解盐胁迫的研究
Pub Date : 2021-10-28 DOI: 10.35691/jbm.1202.0203
Z. Aslam, Ali Ahmad, Anser Ali, A. Sher, M. Sarwar
Salicylic acid (SA) is mainly associated with the regulation of plant growth particularly in salinity stress conditions and this signaling molecule exists in pivotal parts of plant. The objective of this research analysis was to overcome stress condition by distinct foliar treatments of Salicylic acid, for instance, 0 and 100 mM. Foliar applications of SA on maize were rendered on 10 days later than transplanting under a hydroponics experiment. Plants were grown under non-saline (S0= 0 mM NaCl) and saline (S1= 100 mM NaCl) conditions. Evaluation of biochemical, physiological and morphological attributes of maize was rendered after harvesting of plants. The experimental layout of Completely Randomized Design (CRD) under a factorial arrangement with three replications of each treatment was assigned for this study. According to our results it was confirmed that cultivation of maize under saline condition reduced the morphological, physiological and biochemical attributes of plant. However, exogenous application of SA on maize had a positive impact on the above mentioned traits under presence and absence of saline environment. Finally, it was justified that exogenous application of variable concentration of SA significantly improves whole parameters of maize cultivar.
水杨酸(Salicylic acid, SA)主要与植物生长调控有关,特别是在盐胁迫条件下,这种信号分子存在于植物的关键部位。本研究分析的目的是通过不同的水杨酸叶面处理(例如0和100 mM)来克服胁迫条件。在水培试验下,在移栽后10天对玉米进行水杨酸叶面处理。植株在无盐(S0= 0 mM NaCl)和盐水(S1= 100 mM NaCl)条件下生长。对玉米收获后的生化、生理和形态性状进行了评价。本研究采用完全随机设计(CRD)的试验布局,每个处理3个重复。结果表明,在盐渍条件下栽培玉米降低了植株的形态、生理和生化特性。然而,在有盐环境和无盐环境下,外源施用SA对玉米上述性状均有积极影响。结果表明,外源施用变浓度SA能显著提高玉米品种的各项指标。
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引用次数: 1
Undesired Effects of Bioinsecticides Molecules in Wistar Rats: Case of Spirotetramat, Citrulus Colocynthis and Cleome Arabica Extracts 生物杀虫剂分子对Wistar大鼠的不良影响:以螺虫、瓜泥和阿拉比卡茶提取物为例
Pub Date : 2021-10-13 DOI: 10.35691/jbm.1202.0201
Boublata Nour El Iméne, M. Bekhakheche, Sarra Habbachi, F. Saadane, Bouzar Abir
The use of pesticides is becoming an indispensable technique in most agricultural practices, regardless of the level of development of the country. Currently, the pesticides used are often less toxic and more specific and are based on insect physiology. Recent studies indicate that pesticide intoxication induces oxidative stress. This work aims to study the neurobehavioral consequences of the administration of two insecticides currently widely used in agriculture: spirotetramat (an inhibitor of lipid synthesis in insects) and the ethanolic extract of Citrullus colocynthis (a plant endemic to the Algerian Sahara) and the ethanolic extract of Cleome arabica (Capparidaceae) The different behavioral tests (elevated cross maze, open fields and forced swimming) show that the have a significant impact on the degree of anxiety of rodents.These products significantly influence the biochemical parameters (blood sugar, cholesterol, triglycerides, urea, creatinine), the hormone Adreno CorticoTropic Hormone, acetylcholine esterase,
无论一个国家的发展水平如何,在大多数农业实践中,使用农药正成为一项不可或缺的技术。目前,使用的农药通常毒性较小,更具有特异性,并且基于昆虫生理学。近年来的研究表明,农药中毒可引起氧化应激。本研究旨在研究目前广泛用于农业的两种杀虫剂对神经行为的影响:不同的行为实验(高架交叉迷宫、露天场地和强迫游泳)表明,不同的行为实验(高架交叉迷宫、露天场地和强迫游泳)对啮齿动物的焦虑程度有显著影响。这些产品显著影响生化参数(血糖、胆固醇、甘油三酯、尿素、肌酐)、促肾上腺皮质激素、乙酰胆碱酯酶、
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引用次数: 0
Preliminary Checklist of Avifauna of Tamanrasset (South of Algeria) With Two New Records in Algeria 阿尔及利亚南部塔曼拉塞特鸟类初步名录及两项新记录
Pub Date : 2021-09-02 DOI: 10.35691/jbm.1202.0199
B. A. Boulaouad, Ailam Oussama, Telailia Salah, Harzallah Mourad, Ayyach Khaled, Bekkouche Abdelkadir Missoum Mohamed, Soukkou Walid, Boutabia Lamia
Birds constitute a zoological group which is considered to be a good indicator of balance and biological diversity. The aim of this study was to investigate the birds of the region on Tamanresset which is situated in the south of Algeria and has an area of 554.000 km2. Direct observations with camera and a pair of binoculars, supported by ornithological surveys carried out by progressive frequently sampling, in each stations of study area, 44 bird species were observed in this region which fall into 12 orders and 24 families. Among these species, 3 new observations recorded for the 1st time belonging to White-rumped Seedeater (Crithagra leucopygia) and blue-napped mousebird (Urocolius macrourus) along with 2 other species, the rose-ringed parakeet (Psittacula krameri) and the African silver bill (Euodice cantans), are listed as an expansion in this region.
鸟类是一个动物类群,被认为是平衡和生物多样性的良好指标。本研究的目的是调查塔曼雷塞特地区的鸟类,该地区位于阿尔及利亚南部,面积为554,000平方公里。利用相机和双筒望远镜直接观测,辅以渐进式频繁采样的鸟类学调查,在研究区各站点共观测到鸟类44种,隶属于12目24科。在这些物种中,有3种是首次新发现的白背种子鸟(Crithagra leucopygia)和蓝枕鼠鸟(Urocolius macrus),以及另外2种玫瑰环长尾小鹦鹉(Psittacula krameri)和非洲银喙(Euodice cantans)被列为该地区的扩展物种。
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引用次数: 0
Abundance and Distribution of the Forest Cockroaches in Different Algerian Ecosystems 阿尔及利亚不同生态系统中森林蟑螂的丰度和分布
Pub Date : 2021-08-26 DOI: 10.35691/jbm.1202.0196
Z. Hedjouli, W. Habbachi, F. Masna, S. Benhissen, Sarra Habbachi, A. Y. Asloum, A. Tahraoui
Forests have many insect species that are unique to biodiversity and play a crucial role in the functioning of ecosystems. Forest cockroaches are the best example of forest litter insects, which also help decompose fallen leaves. The composition of the Blattoptera fauna can vary from one region to another, depending on the habitat and numerous biotic and abiotic factors In order to study the diversity, abundance, and distribution of forest cockroaches in different Algeria ecosystems, we have selected four sites from three Wilayas: Senalba (Djelfa), Ain achir (Annaba), Seraidi (Annaba), and Lehnaya (El-taref). These sites are classified into two distinct forest groups (Pinus halepensis and Quercus canariensis). In all areas, the samples were collected each month manually, starting from April 2019 until March 2020. We have collected 1231 individuals of cockroaches and describe eight species from all locations: Loboptera ovolobata (Bohn, 1991), Loboptera decipiens (Germar, 1817), Ectobius kervillei (Bolivar, 1907), Dziriblatta nigriventris (Chopard, 1936), Dziriblatta stenoptera (Chopard, 1937), Phyllodromica zebra (Rhen, 1903), Dziriblatta sp. (Chopard, 1936) and some Ectobius (Stephens, 1835) specimens have not yet been identified. With 51% L. decipiens was the most abundant species, followed by L. ovolobata with 27%. Despite that, the number of larvae was substantially higher than the number of adults in all forests examined. However, the abundance and biodiversity of cockroaches in the Djelfa forest were greater than those of the other woods. This study made it possible to characterize the diversity and dynamics of cockroach species populations in different Algerian ecosystems according to diverse Mediterranean forests.
森林中有许多昆虫物种,它们对生物多样性具有独特的意义,在生态系统的功能中起着至关重要的作用。森林蟑螂是森林凋落物昆虫的最好例子,它们也有助于分解落叶。为了研究阿尔及利亚不同生态系统中森林蜚蠊的多样性、丰度和分布,我们从3个省中选择了Senalba (Djelfa)、Ain achir (Annaba)、Seraidi (Annaba)和Lehnaya (El-taref) 4个地点进行研究。这些地点被划分为两个不同的森林类群(halepensis Pinus和canariensis Quercus)。从2019年4月至2020年3月,在所有地区,每个月都人工采集样本。我们收集了1231只蜚蠊,并对8种蜚蠊进行了描述,包括:ovolobata (Bohn, 1991)、looptera decipiens(德国,1817)、Ectobius kervillei (Bolivar, 1907)、Dziriblatta nigriventris (Chopard, 1936)、Dziriblatta stenoptera (Chopard, 1937)、Phyllodromica zebra (Rhen, 1903)、Dziriblatta sp. (Chopard, 1936)和一些尚未鉴定的Ectobius (Stephens, 1835)。其中,脱蚊种类最多(51%),卵叶蛙次之(27%)。尽管如此,在所调查的所有森林中,幼虫的数量大大高于成虫的数量。杰尔法森林的蜚蠊数量和生物多样性均高于其他森林。这项研究使得根据不同的地中海森林描述阿尔及利亚不同生态系统中蟑螂物种种群的多样性和动态成为可能。
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引用次数: 1
Preliminary Inventory and General Aspect of the Distribution of Culicidae Species in the Steppe Region (M'sila, Algeria) 阿尔及利亚M'sila草原地区库蚊科物种分布概况及初步调查
Pub Date : 2021-08-26 DOI: 10.35691/jbm.1202.0197
A. Y. Asloum, S. Benhissen, W. Habbachi, Sarra Habbachi, Z. Hedjouli, Zihad Bouselama, A. Tahraoui
Mosquitoes are important living organisms that participate in the different levels of food chains. However, many families include vector species capable to transmit viruses, bacteria and parasites to both humans and animals, threatening the public health. The goal of this work was to describe the biological diversity of mosquito species in different areas in M'sila; Algeria based on the ecological nature of their habitat. The breeding sites found were 18 distributed in five areas (Berhoum, Bou Saâda, El hamel, Hammam Dhalaa and M'sila). The inventory that was carried out from September to April every year from 2017 to 2019 has revealed 14 species of Culicidae distributed through three genera. The Culiseta longiareolata species was the most predominant, occupying different types of permanent and temporary breeding sites. However, the distribution of species based on the ecological types of these sites revealed: Anopheles cinereus, Anopheles sergentii, Culex brumpti, Culex deserticola, Culex hortensis, Culex impudicus, Culex laticinctus, Culex martinii Culex modestus,Culex perexiguus,Culex pipiens, Culex theileri and Culiseta longiareolata species presence The study of mosquito populations in the M’sila region has never been approached ecologically, biologically and systematically. Focus of this study was devoted to the systematics of Culicidae species, as well as the characterization of their structure, using ecological parameters as well as the study of Spatio-temporal variations, of the Culicidae population.
蚊子是参与不同层次食物链的重要生物。然而,许多科包括能够向人类和动物传播病毒、细菌和寄生虫的病媒物种,威胁着公众健康。这项工作的目的是描述M'sila不同地区蚊子物种的生物多样性;阿尔及利亚基于其生态性质的栖息地。18个繁殖点分布在5个地区(Berhoum、Bou sa da、El hamel、Hammam Dhalaa和M'sila)。2017 - 2019年每年9 - 4月进行调查,共发现库蚊科3属14种。其中以长绒鸡属(Culiseta longiareolata)为优势种,占据了不同类型的永久和临时繁殖地。然而,基于生态类型的蚊种分布显示:存在电影按蚊、塞真按蚊、布朗库蚊、荒漠库蚊、短尾库蚊、粗尾库蚊、浅尾库蚊、马提尼库蚊、温和库蚊、潜行库蚊、淡色库蚊、淡色库蚊和长纹库蚊。本研究的重点是库蚊科的物种分类、结构特征、生态参数以及种群的时空变化研究。
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引用次数: 0
Enhancing the Fertilizer Value of Cattle Manure Using Organic Resources for Soil Fertility Improvement: A Review 利用有机资源提高牛粪肥力的研究进展
Pub Date : 2021-08-26 DOI: 10.35691/jbm.1202.0198
B. E. Ayamba, R. Abaidoo, A. Opoku, N. Ewusi-Mensah
The poor quality of cow dung in Ghana resulting from the opportunistic feeding mode of cattle impedes its adoption due to high quantities needed for application. This calls for nutrient optimization of cattle manure with inexpensive locally available sources of organic inputs that could enhance its quality. Though large volumes of crop residues and other agro-wastes are produced on-farm, they are underutilized and mostly a nuisance. Major food crops take approximately, 44 %, 42 % and 56 % of the total nitrogen (N), phosphorus (P) and potassium (K), respectively and are found in crop residues. There is therefore the need to develop appropriate technologies for utilizing agro-wastes by transforming them into useful “resources” with potentially available plant macro and micronutrients. Common agro-wastes in Ghana include cocoa pod husk (CPH), palm kernel cake (PKC), cattle manure (CM) and poultry manure. Hence, this review sought to explore the use of crop residues and agro-minerals to improve the quality of manure and consequently its fertilizer value for soil fertility improvement and increased crop yield. The review presented an assessment of resource potentials of some crop residues and local agro-mineral as a means of enhancing the quality of CM. Using published data, the review has identified that PKC, rock phosphate (RP) and CPH have high potential for improving the N, P and K contents of CM, respectively. In conclusion, it recommends the need to extensively explore the potential of other commonly available organic resource materials for their efficacy to improve the fertilizer value of cattle manure.
在加纳,由于牛的机会性饲养模式导致牛粪质量差,由于需要大量使用,阻碍了牛粪的采用。这就要求对牛粪进行营养优化,使用当地可获得的廉价有机投入来源,从而提高牛粪的质量。虽然农场产生了大量的作物残茬和其他农业废物,但它们没有得到充分利用,而且大多令人讨厌。主要粮食作物分别约占总氮(N)、磷(P)和钾(K)的44%、42%和56%,这些氮(N)、磷(P)和钾(K)存在于作物残留物中。因此,需要开发利用农业废物的适当技术,将其转化为具有潜在的植物宏量和微量营养素的有用“资源”。加纳常见的农业废弃物包括可可豆荚壳(CPH)、棕榈仁饼(PKC)、牛粪(CM)和禽粪。因此,本文试图探索利用作物残茬和农用矿物来改善肥料质量,从而提高土壤肥力和作物产量的肥料价值。本文介绍了作物残茬和当地农用矿物的资源潜力评价,作为提高农用矿物质量的手段。利用已发表的数据,本综述发现PKC、RP和CPH分别对CM的N、P和K含量有较大的提高潜力。综上所述,有必要广泛探索其他常用有机资源材料在提高牛粪肥效方面的潜力。
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引用次数: 2
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Journal of Bioresource Management
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