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Assessment of Available Manganese in Milk by Using fodders Grown in Long-Term Wastewater Irrigated Soil 利用长期废水灌溉土壤中生长的饲料评价牛奶中的有效锰
Pub Date : 2021-05-26 DOI: 10.35691/JBM.1202.0185
M. Munir, K. Ahmad, Z. Khan, A. Zahra, A. Ashfaq, I. Malik, M. Akhtar, H. Bashir, M. U. Awan
Heavy metals are considered as most important contaminations due to industrialization of countries and an influence on its existence in soil, plant and milk. A study was carried out to check manganese content in soil, forage and milk at three sites of city Jhang, Punjab, Pakistan. All samples (milk, soil, water, fodder plants and ground water) were analyzed for manganese by Atomic Absorption Spectrophotometer. Different health indices were also studied to check Mn flow in food chain. Level of Mn in samples was found within acceptable limits. Manganese level was higher in soil samples collected from Site-III than other sites. Manganese showed higher value (2.595 to 10.402 mg/kg) in soil than other samples. Fodders were found to accumulate manganese from 0.008 to 0.022 mg/kg. Manganese concentration was found to be 0.1482 to 1.241 mg/L, 0.164 to 0.9708 mg/L in water and milk, respectively. BCF and PLI values for manganese were also found to be less than 1. Estimated daily intake (EDI) and THQ of manganese are found within permissible limits in milk of cows feeding on fodders irrigated with wastewater and ground water. So, use of wastewater for irrigation purpose should be properly checked due to possible toxic effects.
由于各国的工业化及其在土壤、植物和牛奶中的存在,重金属被认为是最重要的污染物。对巴基斯坦旁遮普省Jhang市三个地点的土壤、饲料和牛奶中的锰含量进行了研究。用原子吸收分光光度计对所有样品(牛奶、土壤、水、饲料植物和地下水)进行锰含量分析。还研究了不同的健康指标来检测食物链中的锰流量。样品中锰含量在可接受范围内。iii站点土壤样品中锰含量高于其他站点。土壤中锰含量最高,为2.595 ~ 10.402 mg/kg。饲料中锰的累积量为0.008 ~ 0.022 mg/kg。水和牛奶中锰的浓度分别为0.1482 ~ 1.241 mg/L、0.164 ~ 0.9708 mg/L。锰的BCF和PLI值也小于1。在以废水和地下水灌溉的饲料喂养的奶牛的牛奶中,锰的估计每日摄入量(EDI)和THQ均在允许范围内。因此,应适当检查用于灌溉目的的废水,因为可能存在毒性作用。
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引用次数: 2
Phenotypic Plasticity and Parental Effect on Rearing of Two Diverse Habitat Environment for Laboratory Reared Sympetrum Meridionale 两种不同生境环境下实验室饲养的子午线花的表型可塑性及亲本效应
Pub Date : 2021-05-26 DOI: 10.35691/JBM.1202.0186
Imane Halassi, A. Elafri, Ismahan Halassi, H. Amari, M. Houhamdi
Laboratory observations on rearing experiment of Odonata serve to answer many evolutionary and ecological questions. In order to evidences the role of species parental habitat provenience in the development behaviour of their offspring, we surveyed several life history traits of two rearing populations of Sympetrum meridionale (Anisoptera: Sympetrinae), coming from two different habitats across north-eastern Algeria. The first one is a RAMSAR wetland called ‘Mekhada’ (a perennial water body), and the second one is a temporary pond located at “Maouna” Mountain (1400 m altitude). Overall, the development patterns of the two populations of dragonflies vary with the type of habitat the parental generation of the species occupy (Factorial ANCOVA: all p < 0.05). Firstly, egg mortality was very low in dragonfly population inhabiting the RAMSAR wetland compared of those belonging to Maouna Mountain. Secondly new-borne larvae stemming from females inhabiting the Mekhada wetland develop more slowly than did those coming from the “Maouna” Mountain pond. Finally, larvae of Sympetrum meridionale stemming from females inhabiting the temporary wetland were heavier than those inhabiting the perennial wetland. Such studies will ads considerably to our understanding of the mechanisms that are responsible for possible effects of environmental changes on life history traits of dragonflies across the southern part of their distribution range.
对大腹蛇饲养实验的实验室观察有助于回答许多进化和生态学问题。为了证明亲本栖息地在其后代发育行为中的作用,我们调查了来自阿尔及利亚东北部两个不同栖息地的子午对称蝽(Sympetrum meridionale,异翅目:对称蝽科)的几个生活史特征。第一个是RAMSAR湿地“Mekhada”(多年生水体),第二个是位于“Maouna”山(海拔1400米)的临时池塘。总体而言,两种群的发育模式随亲代所占生境类型的不同而不同(析因方差分析:均p < 0.05)。首先,与毛那山的蜻蜓种群相比,拉姆萨尔湿地的蜻蜓种群的卵死亡率非常低。其次,梅卡达湿地雌鱼的幼虫发育速度比毛纳山池塘雌鱼的幼虫发育速度慢。此外,居住在临时湿地的雌虫所产子午线双翅虫的幼虫比居住在多年生湿地的雌虫重。这些研究将大大有助于我们了解环境变化对蜻蜓分布范围南部地区生活史特征可能产生影响的机制。
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引用次数: 1
Hydrochemical Assessment of Groundwater around Lapite Dumpsite for Irrigation Water Quality in Ibadan, Southwestern Nigeria 尼日利亚西南部伊巴丹Lapite垃圾场周边地下水水化学评价对灌溉水质的影响
Pub Date : 2021-05-26 DOI: 10.35691/JBM.1202.0184
O. O. Akintola, G. O. Adeyemi, A. Bodede, O. Adekoya, K. Babatunde
Due to the increase in population and industrialization growth, most countries in the world depend on groundwater to meet agriculture demands for food production. The increase in water contamination due to indiscriminate solid wastes has necessitated the assessment of water quality and its suitability for agricultural usage. Twenty four groundwater and ten stream water samples were randomly collected from the downslope and upslope side of the dumpsite for all the major physio-chemical parameters. The pH of water samples indicates slightly acidic to alkaline in nature. High concentrations of nitrate, total dissolved solids and electrical conductivity suggest the impact of the waste on the water resource. Assessment of irrigation water quality based on Sodium Adsorption Ratio (SAR), Soluble Sodium Percentage (SSP), Permeability Index (PI) and Magnesium content (MC) indicated that most of the water samples fall within the permissible standard for irrigation water. Thus, the water should be used with caution on crops for long time agricultural sustainability.
由于人口的增长和工业化的发展,世界上大多数国家都依赖地下水来满足农业对粮食生产的需求。由于固体废物造成的水污染日益严重,有必要对水质及其是否适合农业使用进行评估。在排土场下坡和上坡随机采集了24个地下水样本和10个水样,进行了主要理化参数的测定。水样的pH值显示为微酸性至碱性。高浓度的硝酸盐、总溶解固体和电导率表明废物对水资源的影响。根据钠吸附比(SAR)、可溶性钠百分率(SSP)、渗透性指数(PI)和镁含量(MC)对灌溉水水质进行评价,结果表明,大部分水样均在灌溉水允许标准范围内。因此,为了农业的长期可持续性,应该谨慎地将水用于作物上。
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引用次数: 2
Profile of Antimicrobial Susceptibility from Cattles’s Milk Isolates Suffering from Mastitis in District Lahore 拉合尔地区乳腺炎牛乳分离株抗菌药物敏感性分析
Pub Date : 2021-05-05 DOI: 10.35691/JBM.1202.0180
Muhammad Imran, Iqra Rehman, A. Q. K. Sulehria, Yasser Mustafa Butt, A. Khan, Aziza Ziauddin
Mastitis is highly prevalent infection in cattle causing cost-effective loss in dairy milk production. Escherichia coli is the most frequently isolated bacteria causing mastitis worldwide. The current study was performed to investigate the mastitis prevalence and effect of different antibiotics against pathogens causing it. In sum, 216 milk samples were collected randomly including 108 each from both cows and buffaloes. These samples were subjected to Surf Field Mastitis Test for prevalence of mastitis that found 18.50% and 23.14% in buffaloes and cows respectively. Mastitis was more prevalent both in buffaloes and cows at the age of 9-10 years that was reported in 35% and 32% respectively. The affected buffaloes showed 65% samples containg watery fluid having blood, pus and mucus whereas 36% samples of affected cows had mucus and watery fluid. Pus and blood content were found to be 12% and 16% respectively. Sub-clinical mastitis was prevalent as 56% and 55% in cows and buffaloes respectively while acute mastitis as 4% and 5% in similar fashion. In both groups Right-for part of udder was frequently affected than any other. Among commonly used four antibiotics, Norfloxacine was observed the most effective antibiotic to control mastitis as compared to Gentamycin, Penicillin and ciprofloxacin. The benefit of this study is to diagnose mastitis and use to isolate pathogens for further processing like antibiotic sensitivity and resistance. This study will help veterinarians in better selection of antibiotic to control mastitis.
乳腺炎是牛中非常普遍的感染,造成牛奶生产的成本效益损失。大肠杆菌是世界上最常见的引起乳腺炎的分离细菌。本研究旨在调查乳腺炎的患病率和不同抗生素对引起乳腺炎的病原体的作用。共随机采集牛奶样品216份,其中奶牛和水牛各108份。对这些样品进行乳区乳炎测试,发现水牛和奶牛的乳炎患病率分别为18.50%和23.14%。乳腺炎在9-10岁的水牛和奶牛中更为普遍,分别为35%和32%。65%的受感染水牛样本含有含有血液、脓液和粘液的水样液体,而36%的受感染奶牛样本含有粘液和水样液体。脓液和血液含量分别为12%和16%。奶牛和水牛的亚临床乳腺炎患病率分别为56%和55%,急性乳腺炎患病率分别为4%和5%。在两组中,Right-for部分乳房比其他部位更容易受到影响。在常用的4种抗生素中,诺氟沙星对乳腺炎的控制效果优于庆大霉素、青霉素和环丙沙星。这项研究的好处是诊断乳腺炎,并用于分离病原体进行进一步处理,如抗生素敏感性和耐药性。本研究将有助于兽医更好地选择抗生素来控制乳腺炎。
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引用次数: 2
Arsenic and Cadmium Risk Assessment in a Domestic Wastewater Irrigated Area Using Samples of Water, Soil and Forages as Indicators 以水、土壤和牧草样品为指标的生活污水灌区砷镉风险评价
Pub Date : 2021-05-05 DOI: 10.35691/JBM.1202.0182
Z. Khan, K. Ahmad, Musarrat Batool, I. Malik, H. Bashir, M. Munir, A. Ashfaq, Pervaiz Akhter, Sonaina Nazar, M. Akhtar, M. Akhtar, M. Nadeem, M. U. Awan
The recent research was performed to investigate the toxicity of As and Cd in suburban area of Sargodha, Punjab, Pakistan. Water, soil and forage samples were collected for this purpose in summer and winter season and analysis was done via wet digestion to determine the concentration of selected heavy metals. The mean concentration of As and Cd in water was found above the permissible maximum limit. Mean concentration of Cd in soil and forages was lower than the allowed limit whereas As which was higher than the PML in forages. So, continuous use of domestic wastewater for irrigation purpose should be avoided in order to secure the area from any hazard in near future.
最近对巴基斯坦旁遮普省萨戈达郊区砷和镉的毒性进行了研究。为此,在夏季和冬季采集了水、土壤和饲料样品,并通过湿消化法进行分析,以确定选定重金属的浓度。水中砷和镉的平均浓度超过了允许的最大限度。土壤和牧草中Cd的平均浓度低于允许值,而As高于允许值。因此,为了确保该地区在不久的将来免受任何危害,应避免将生活废水用于灌溉目的。
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引用次数: 1
Agrobacterium Mediated Transformation Optimizations for Sugarcane (Saccharum Officinarum L.) Cultivar SPF-234 with Direct Organogenesis 农杆菌介导的甘蔗转化优化直接器官发生的品种SPF-234
Pub Date : 2021-05-05 DOI: 10.35691/JBM.1202.0181
M. Nawaz, N. Iqbal, Rabia Hameed, Mehwish Mehwish, S. Jamil
Sugarcane (Saccharum officinarum L.) is the most important food and energy crop worldwide. In the present study, an efficient Agrobacterium mediated transformation and regeneration system for sugarcane cultivar SPF-234 was established. Agrobacterium tumefaciens strains EHA101and LBA4404 using vector pIG121 Hm, having GUS, HPTII and NPTII genes were used. Polymerase chain reaction (PCR) and histochemical assays confirmed the GUS gene expression. A 620 bp fragment from GUS positive plants was amplified. The GUS expressing putative transformants were 35% of the total plants formed under 30 minute immersion time and 72 hr of incubation period. The co-cultivation media having 60 μM acetosyringone produced 66% GUS expressing plants for LBA4404 and 58% for EHA101. The maximum average number of directly produced shoot (59.5%) from leaf explant was in M6 media having 1.00 mg/l 6-Benzylaminopurine (BAP) and 2.5 mg/l Naphthaleneacetic acid (NAA). A significant decrease (17%) was observed when auxin (NAA) concentration was increased to 4.0 mg/l. The best response of shoot elongation was observed in SE4 media having equal concentration (2.00 mg/l) of both kinetin and BAP. Increased concentrations of kinetin significantly decreased shoot elongation of the subject cultivar. Agrobacterium strain LBA4404 performed better for genetic transformation of the said sugarcane cultivar.This quick and less expensive transformation and direct regeneration system could be exploited for sugarcane on commercial scale in general, and for this elite cultivar in particular.
甘蔗(Saccharum officinarum L.)是全球最重要的粮食和能源作物。本研究建立了一种高效的农杆菌介导的甘蔗品种SPF-234转化再生体系。采用载体为pIG121 Hm的农杆菌eha101和LBA4404菌株,分别具有GUS、HPTII和NPTII基因。聚合酶链反应(PCR)和组织化学检测证实GUS基因表达。从GUS阳性植株中扩增出620 bp的片段。在浸泡时间为30分钟、孵育时间为72小时的条件下,表达推定转化体的GUS占植株总数的35%。在含有60 μM乙酰丁香酮的共培养培养基中,LBA4404和EHA101分别有66%和58%的GUS表达植株。在6-苄基氨基嘌呤(BAP)浓度为1.00 mg/l、萘乙酸(NAA)浓度为2.5 mg/l的M6培养基上,叶片外植体直接产芽数最高(59.5%);当生长素(NAA)浓度增加到4.0 mg/l时,细胞凋亡率显著降低(17%)。动素和BAP浓度相等(2.00 mg/l)的SE4培养基对芽伸长的响应最好。动素浓度的增加显著降低了主体品种的茎伸长。农杆菌LBA4404对该甘蔗品种的遗传转化效果较好。这种快速、廉价的转化和直接再生系统一般可用于商业规模的甘蔗,特别是该优良品种。
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引用次数: 0
Challenges and Opportunities in Food Safety-A Review 食品安全的挑战与机遇
Pub Date : 2021-04-23 DOI: 10.35691/JBM.1202.0177
Iram Asim, H. Yasmeen
Food-borne diseases are the group of disorders that are caused by consuming food having microbial existence in it. So safe food handling is to make sure the lessening of detrimental effects in growth to the packaging of food to minimize health issues on consumers which otherwise can lead to large scale disease outburst. This review concludes the findings of the studies on how food is being handled from farm to fork, how airlines are contributing towards the spreading of diseases, how any negligence in any one of the steps can cause havoc to mankind in the light of the recent coronavirus pandemic. This review suggests the methods for the detection of food-borne viruses and the challenges for the regulation of zoonotic outbursts. This review recommends strict regulation, updating of the food handling policies, and ways to control emerging infectious diseases in relation to food.
食源性疾病是由于食用含有微生物的食物而引起的一组疾病。所以安全的食品处理是为了确保减少对食品包装的有害影响以最大限度地减少消费者的健康问题否则会导致大规模的疾病爆发。这篇综述总结了以下研究的结果:食品从农场到餐桌的处理方式、航空公司如何促进疾病的传播、鉴于最近的冠状病毒大流行,任何一个步骤的任何疏忽都可能对人类造成严重破坏。本文综述了食源性病毒的检测方法及人畜共患疫情控制面临的挑战。本综述建议严格监管,更新食品处理政策,以及控制与食品有关的新发传染病的方法。
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引用次数: 1
Breeding Ecology and Nest- Site Selection of Turtle Doves (Streptopelia turtur) in Three New Orchard Habitats 三种果园新生境斑鸠的繁殖生态学及巢址选择
Pub Date : 2021-04-23 DOI: 10.35691/JBM.1202.0175
Thilelli Aitouakli, E. Bensaci
The main aim of our work was to investigate the breeding parameters in three orchard types in Algeria (apple, cherry and nectarine) for better conservation of existing species. A total of 149 active Turtle dove nests were monitored in these man–made agro-systems. Egg laying occurred from early May and continued until mid-August. Egg laying started later in cherry trees and stopped earlier in nectarines. Nest density was higher in apple orchards. Nests were located higher in nectarine. Clutch size was similar among orchard types. Northeast was the dominant orientation in all orchards. Breeding success was higher than that recorded in former studies. Desertion was the main cause of nest failure. The apple orchards are the preferred breeding area for turtle dove in this region.
我们工作的主要目的是调查阿尔及利亚三种果园类型(苹果、樱桃和油桃)的育种参数,以便更好地保护现有物种。在这些人工农业系统中,共监测到149个活跃的斑鸠巢。5月初开始产卵,一直持续到8月中旬。樱桃树的产卵时间较晚,油桃的产卵时间较早。苹果园巢密度较高。巢位于油桃较高的地方。不同果园类型的卵落大小相似。东北是所有果园的优势方向。繁殖成功率高于以前的研究记录。遗弃是巢失败的主要原因。苹果园是本地区斑鸠的首选繁殖地。
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引用次数: 1
Ecology of Patas Monkey (Erythrocebus Patas) in Buffer Zone Ranges, Old Oyo National Park, Nigeria 尼日利亚老奥约国家公园,缓冲地带山脉中野猴的生态学
Pub Date : 2021-04-23 DOI: 10.35691/JBM.1202.0178
S. Halidu, O. Adebayo, J. Chikezie, A. Ibrahim, Olushola Adedeji
The buffer zones of Nigerian protected areas are poorly managed. Patas monkey (Erythrocebus patas) was studied in fact owing to its dearth of information on its ecology and existence in South-West Nigeria National Park. Therefore, it was necessary to examine E. patas spatial distribution and population structure in the Old Oyo National Park buffer zone ranges, situated in South-West Nigeria and as well know its dietary uptake alongside delineating its various activities. Observations were positioned on direct census methods. A total of 60 individuals of E. patas (23 female, 21 male, and 16 young individuals) were sighted within the study period (March to July). The results showed that the highest number of sighted E. patas (18 individuals) was recorded in the month of March while the Marguba range had the highest percentage of sighted E. patas (31.7%). Thirteen plant species were identified as food matters of E. patas while the major activity observed by the majority of E. patas was found feeding on the identified food matters. The food matters consumed were plant parts which included the leaves, flowers, gum, enflamed spikes, and fruits. Actions to uphold and guard the remaining populace of E. patas in the Park and buffer zones should be adequately fortified.
尼日利亚保护区的缓冲地带管理不善。事实上,由于缺乏关于其在尼日利亚西南部国家公园的生态和存在的信息,研究了Patas猴(Erythrocebus Patas)。因此,有必要研究位于尼日利亚西南部的Old Oyo国家公园缓冲区范围内的野田鼠的空间分布和种群结构,并了解其饮食摄取情况,同时描绘其各种活动。观察结果采用直接人口普查方法。在研究期间(3 - 7月)共发现白蛉60只,其中雌虫23只,雄虫21只,幼虫16只。结果表明,3月份白斑田鼠最多(18只),而马古巴田鼠最多(31.7%)。确定了13种植物是patas的食物来源,大多数patas的主要活动是以这些食物为食。食用的食物是植物的一部分,包括叶子、花、树胶、燃烧的穗状物和果实。保护和保护公园和缓冲区内剩余的野田鼠的行动应该得到充分加强。
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引用次数: 0
The Phytotherapeutic Arsenal in the Guerbes-Sanhadja Wetlands Complex (North East of Algeria) Guerbes-Sanhadja湿地复合体的植物治疗库(阿尔及利亚东北部)
Pub Date : 2021-04-23 DOI: 10.35691/JBM.1202.0179
Belaidi Abdelouahab, Beghami Yassine, F. M. Vázquez, S. Nabila, Bendif Hamdi
This study documents indigenous medicinal flora utilization, and was carried out in the Guerbes-Sanhadja wetland complex, between January 2018 and December 2020. The detailed information was gathered from users of medicinal plants mainly based on semistructured interviews. A total of 102 plant species distributed in 48 families were found in practice by the natives for fulfilling their daily life requirements. The most frequently cited plants are: Myrtus communis L. (158 citations), Pistacia lentiscus L. (122) and Thymus vulgaris L. (97). Most of the medicinal species listed were herbs (68.93%). The phanerophytes (33.33%) and hemicriptophytes (27.45%) were the principal species used by the local population of the complex to treat different diseases. And finally, the dependence of the local population on medicinal plants prompted the establishment of a complex management plan in order to promote and protect them through their rational use and domestication.
该研究记录了当地药用植物的利用情况,并于2018年1月至2020年12月在Guerbes-Sanhadja湿地群进行。详细信息主要通过半结构化访谈从药用植物使用者那里收集。当地人在实践中发现了48科102种植物,以满足他们的日常生活需要。最常被引用的植物是桃金娘(Myrtus communis L.)(158次引用)、黄连木(Pistacia lentiscus L.)(122次引用)和麝香(thyymus vulgaris L.)(97次引用)。所列药用种类以中草药居多(68.93%)。当地人群治疗不同疾病的主要种类是显生植物(33.33%)和半植植物(27.45%)。最后,当地人口对药用植物的依赖促使建立了一个复杂的管理计划,以便通过合理使用和驯化来促进和保护它们。
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引用次数: 0
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Journal of Bioresource Management
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