O. O. Akintola, Adeboyin Funmi Aderounmu, I. O. Abiola, Kolawole Emmanuel Abodurin, T. Adeniran, F. Agboola, Oluwayemisi Samuel Olokeogun
Okoubaka aubrevillei is an indigenous and sacred tropical tree in Africa. It is rare with allelopathic properties and has relatively little information available in terms of what is responsible for its usage for medicinal and traditional usage. The phytochemical screening and quantitative analysis of the compounds in the barks and leaves of Okoubaka aubrevillei was determined to ascertain and establish its earlier claimed usage as traditional and modern medicine. The most important classes of secondary metabolites (phytochemical compounds) specifically alkaloids, flavonoids, tannins, saponins, anthraquinnes, mucilages, oses, holosides, coumarin and glycosides were detected in both, the leaves and barks of the tree. Quantitative determination of the phytochemical compounds found in the barks and leaves of the tree revealed that the leaves of O. aubreville tree had significantly higher values of alkaloids, flavonoids and glycosides than barks. Saponins and anthraquinnes were found to be significantly more in barks than in leaves. However, there was no significant difference found in the values of tannins, mucilages, oses and holosides and coumarin in barks and leaves of O. aubreville trees.
{"title":"Quantitative Analysis of Phytochemical Compounds in Barks and Leaves of Okoubaka Aubrevillei Collected from Iwo, Southwestern Nigeria","authors":"O. O. Akintola, Adeboyin Funmi Aderounmu, I. O. Abiola, Kolawole Emmanuel Abodurin, T. Adeniran, F. Agboola, Oluwayemisi Samuel Olokeogun","doi":"10.35691/jbm.0202.0146","DOIUrl":"https://doi.org/10.35691/jbm.0202.0146","url":null,"abstract":"Okoubaka aubrevillei is an indigenous and sacred tropical tree in Africa. It is rare with allelopathic properties and has relatively little information available in terms of what is responsible for its usage for medicinal and traditional usage. The phytochemical screening and quantitative analysis of the compounds in the barks and leaves of Okoubaka aubrevillei was determined to ascertain and establish its earlier claimed usage as traditional and modern medicine. The most important classes of secondary metabolites (phytochemical compounds) specifically alkaloids, flavonoids, tannins, saponins, anthraquinnes, mucilages, oses, holosides, coumarin and glycosides were detected in both, the leaves and barks of the tree. Quantitative determination of the phytochemical compounds found in the barks and leaves of the tree revealed that the leaves of O. aubreville tree had significantly higher values of alkaloids, flavonoids and glycosides than barks. Saponins and anthraquinnes were found to be significantly more in barks than in leaves. However, there was no significant difference found in the values of tannins, mucilages, oses and holosides and coumarin in barks and leaves of O. aubreville trees.","PeriodicalId":115980,"journal":{"name":"Journal of Bioresource Management","volume":"51 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2020-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115227105","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
There is an increasingly high suspicion of occupational health diseases amongst workers in sawmill factories. Hence this study aimed to determine the microbial diversity in some sawmill factories in Okada, Edo State, Nigeria to evaluate potential implications of the factory’s processing area on the health of the workers and wood merchants. Bacteria and fungi count in the bioaerosols within the processing area of each factory was performed with the passive air sampling technique. While in the sawdust samples, microbial counts were carried out with the pour plate technique. Identification of the microbes was performed with macroscopic and microscopic examinations as well as standard phenotypic tests. Mean total viable count (TVC) and total fungi count (TFC) of bioaerosols in the air within the processing area of the sawmill factories were found to be greater than 4162.99 CFU/m while mean total coliform count (TCC) was reported as 756.28 CFU/m. Fisher (F) one-way ANOVA test of the TVC and TCC dataset of bioaerosols from the four sawmill factories indicated no significant difference (p = 0.77 and 0.83 for TVC and TFC respectively) in the mean TVC and TFC of the bioaerosols. Bacterial (Micrococcus, Staphylococcus, Bacillus, Klebsiella and Serratia) and fungal (Saccharomyces, Aspergillus, Cladosporium and Penicillium) species were isolated from the bioaerosols and sawdust samples. Results of this study indicated that workers in the sawmill factories and other wood merchants who visit sawmill processing area are most likely exposed to airborne contaminants that may cause occupational diseases such as ophthalmic irritations and dermatitis. Hence it is recommended that sawmill workers and other visitors wear personal protective gadgets in the processing area of the sawmill factories.
{"title":"Microbial Diversity in the Sawmill Environment: Implications on the Health of Sawmill Workers and Merchants, Nigeria","authors":"E. J. Okafor-Elenwo, O. Imade, O. Izevbuwa","doi":"10.35691/jbm.0202.0143","DOIUrl":"https://doi.org/10.35691/jbm.0202.0143","url":null,"abstract":"There is an increasingly high suspicion of occupational health diseases amongst workers in sawmill factories. Hence this study aimed to determine the microbial diversity in some sawmill factories in Okada, Edo State, Nigeria to evaluate potential implications of the factory’s processing area on the health of the workers and wood merchants. Bacteria and fungi count in the bioaerosols within the processing area of each factory was performed with the passive air sampling technique. While in the sawdust samples, microbial counts were carried out with the pour plate technique. Identification of the microbes was performed with macroscopic and microscopic examinations as well as standard phenotypic tests. Mean total viable count (TVC) and total fungi count (TFC) of bioaerosols in the air within the processing area of the sawmill factories were found to be greater than 4162.99 CFU/m while mean total coliform count (TCC) was reported as 756.28 CFU/m. Fisher (F) one-way ANOVA test of the TVC and TCC dataset of bioaerosols from the four sawmill factories indicated no significant difference (p = 0.77 and 0.83 for TVC and TFC respectively) in the mean TVC and TFC of the bioaerosols. Bacterial (Micrococcus, Staphylococcus, Bacillus, Klebsiella and Serratia) and fungal (Saccharomyces, Aspergillus, Cladosporium and Penicillium) species were isolated from the bioaerosols and sawdust samples. Results of this study indicated that workers in the sawmill factories and other wood merchants who visit sawmill processing area are most likely exposed to airborne contaminants that may cause occupational diseases such as ophthalmic irritations and dermatitis. Hence it is recommended that sawmill workers and other visitors wear personal protective gadgets in the processing area of the sawmill factories.","PeriodicalId":115980,"journal":{"name":"Journal of Bioresource Management","volume":"19 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2020-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130053960","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Cake of shea nut is an agroforestry by-product and residue after fat extraction from shea nuts for fat with no economic value and its increasing output has become an environmental issue lately. A 30-day study was conducted to investigate the apparent nutrient digestibility and carcass yield of broiler chicken fed cooked cake of shea nut diets of different fermentation periods. A total of 144 unsexed Arbor Acres Plus day-old broiler chickens were divided into four dietary treatments with four replicates of nine birds each in a completely randomized design. The results showed that fermentation enhanced the nutrient profile of cake of shea nut meal especially concerning crude protein and crude fiber as compared to raw cake of shea nut meal. The treatment values for crude protein and crude fiber digestibility showed significant (p < 0.05) differences amongst the dietary treatments while other nutrient parameters measured were not significantly (p > 0.05) different. There was no significant (p > 0.05) difference amid the treatment means for the control (carcass, breast and liver yield) and the carcass, breast and liver yield of broiler chickens fed diets containing different periods of fermented-cooked cake of shea nut meal.
{"title":"Apparent Nutrient Digestibility and Carcass Yield of Broiler Chickens Fed Cooked Shea Nut Cake Diets of Different Fermentation Periods","authors":"O. Adebayo, J. Chikezie, S. Halidu","doi":"10.35691/jbm.0202.0145","DOIUrl":"https://doi.org/10.35691/jbm.0202.0145","url":null,"abstract":"Cake of shea nut is an agroforestry by-product and residue after fat extraction from shea nuts for fat with no economic value and its increasing output has become an environmental issue lately. A 30-day study was conducted to investigate the apparent nutrient digestibility and carcass yield of broiler chicken fed cooked cake of shea nut diets of different fermentation periods. A total of 144 unsexed Arbor Acres Plus day-old broiler chickens were divided into four dietary treatments with four replicates of nine birds each in a completely randomized design. The results showed that fermentation enhanced the nutrient profile of cake of shea nut meal especially concerning crude protein and crude fiber as compared to raw cake of shea nut meal. The treatment values for crude protein and crude fiber digestibility showed significant (p < 0.05) differences amongst the dietary treatments while other nutrient parameters measured were not significantly (p > 0.05) different. There was no significant (p > 0.05) difference amid the treatment means for the control (carcass, breast and liver yield) and the carcass, breast and liver yield of broiler chickens fed diets containing different periods of fermented-cooked cake of shea nut meal.","PeriodicalId":115980,"journal":{"name":"Journal of Bioresource Management","volume":"14 12 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2020-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115721366","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
H. Ramzi, Khammar Hichem, Redjaimia Lylia, Merzoug Djemoi, S. Menouar
Groundwater of Oum-El-Bouaghi and its surroundings hosts a variety of microflora and fauna. This study investigated the relationship between the effect of human activity and the biodiversity and distribution of aquatic fauna in two semi-arid regions Sighus region (Oum-El-Bouaghi) and El Rahmouni (Constantine) in north-eastern Algeria. Fourteen wells and six springs were studied in two hydrographic basins, that of Constantine and Seybouse Melegue. Significant differences were revealed between the wells and springs in the two watersheds, making it possible to distinguish four groups of wells and two groups of springs. The overall faunal richness of the stations appeared to be weakly correlated with water quality, but on the other hand, the specific richness of the stygoby fauna, and even more so the abundance of stygoby species, decreased when water quality deteriorated.
{"title":"Impact of Anthropic Pressure on the Quality and Diversity of Groundwater in the Region of Sighus Oum-El-Bouaghi and El Rahmounia, Algeria.","authors":"H. Ramzi, Khammar Hichem, Redjaimia Lylia, Merzoug Djemoi, S. Menouar","doi":"10.35691/jbm.0202.0142","DOIUrl":"https://doi.org/10.35691/jbm.0202.0142","url":null,"abstract":"Groundwater of Oum-El-Bouaghi and its surroundings hosts a variety of microflora and fauna. This study investigated the relationship between the effect of human activity and the biodiversity and distribution of aquatic fauna in two semi-arid regions Sighus region (Oum-El-Bouaghi) and El Rahmouni (Constantine) in north-eastern Algeria. Fourteen wells and six springs were studied in two hydrographic basins, that of Constantine and Seybouse Melegue. Significant differences were revealed between the wells and springs in the two watersheds, making it possible to distinguish four groups of wells and two groups of springs. The overall faunal richness of the stations appeared to be weakly correlated with water quality, but on the other hand, the specific richness of the stygoby fauna, and even more so the abundance of stygoby species, decreased when water quality deteriorated.","PeriodicalId":115980,"journal":{"name":"Journal of Bioresource Management","volume":"46 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2020-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"124826979","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Redjaimia Lylia, H. Ramzi, Khammar Hichem, Merzoug Djemoi, S. Menouar
The biodiversity and quality of subterranean waters were comparatively studied in the Tarf plain near Oum-El-Bouaghi and in the Ksar S’bahi in Oum-El-Bouaghi, in North-eastern Algeria. For this purpose, physicochemical and faunistic analyses were carried out on the water of ten stations located in the area of Tarf, and thirteen in the area of S’bahi. In the wells of Tarf, the average stygobiologic diversity was relatively high in the wells located upstream the dumping site from the city where the groundwater presented low contents of nitrates and orthophosphates. In contrast, the wells located in the spreading zone of Tarf wastewaters were characterized by the scarcity or the absence of stygobic species; in these latter wells, the water was highly polluted. It was rich in nitrates, nitrites, ammonium, and the conductivity was rather high. In the area of S’bahi, the faunistic inventory recorded ten species, some of which were living in hot springs. The subterranean water was highly mineralized. In the two studied areas, biodiversity decreased when well water was locally polluted.
对阿尔及利亚东北部Oum-El-Bouaghi附近的Tarf平原和Oum-El-Bouaghi的Ksar S 'bahi地下水的生物多样性和水质进行了比较研究。为此目的，对位于塔夫地区的10个站和S 'bahi地区的13个站的水进行了物理化学和动物分析。在城市排土场上游水体中，水体中硝酸盐和正磷酸盐含量较低的水体中，水体中茎尖生物多样性平均值较高。相反，位于焦油废水扩散区的井具有缺乏或缺乏粘菌的特征;后几口井里的水被严重污染了。它含有丰富的硝酸盐、亚硝酸盐、铵，电导率较高。在S 'bahi地区，动物群调查记录了10种，其中一些生活在温泉中。地下水高度矿化。在两个被研究的地区，当井水被当地污染时，生物多样性下降。
{"title":"Groundwater Quality in Two Semi-Arid Areas of Algeria: Impact of Water Pollution on Biodiversity","authors":"Redjaimia Lylia, H. Ramzi, Khammar Hichem, Merzoug Djemoi, S. Menouar","doi":"10.35691/jbm.0202.0137","DOIUrl":"https://doi.org/10.35691/jbm.0202.0137","url":null,"abstract":"The biodiversity and quality of subterranean waters were comparatively studied in the Tarf plain near Oum-El-Bouaghi and in the Ksar S’bahi in Oum-El-Bouaghi, in North-eastern Algeria. For this purpose, physicochemical and faunistic analyses were carried out on the water of ten stations located in the area of Tarf, and thirteen in the area of S’bahi. In the wells of Tarf, the average stygobiologic diversity was relatively high in the wells located upstream the dumping site from the city where the groundwater presented low contents of nitrates and orthophosphates. In contrast, the wells located in the spreading zone of Tarf wastewaters were characterized by the scarcity or the absence of stygobic species; in these latter wells, the water was highly polluted. It was rich in nitrates, nitrites, ammonium, and the conductivity was rather high. In the area of S’bahi, the faunistic inventory recorded ten species, some of which were living in hot springs. The subterranean water was highly mineralized. In the two studied areas, biodiversity decreased when well water was locally polluted.","PeriodicalId":115980,"journal":{"name":"Journal of Bioresource Management","volume":"8 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2020-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122989804","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Hina Rafiq, Fakhar -i-Abbas, Fakhra Nazir, F. M. Khan
Doves and Pigeons are the members of living family Columbidae (Order: Columbiformes) having a wide range of taxonomic diversity and geographic distribution. Seven species with one sample each of family Columbidae were collected via random sampling from different districts of Pakistan to carry out this study. The targeted gene region was sequenced and identified by using BLAST tool at National Center for Biotechnology Information (NCBI). CLUSTALW was used for sequence alignment and MEGA6 for reconstruction of phylogenetic trees to predict the effective ancestry of different Columbidae species. The following phylogenetic trees were obtained i.e. Maximum Likelihood tree, Neighborhood joining tree, Maximum parsimony tree and UPGMA tree. In the current study, COI gene barcoding and phylogenetic analysis of family Columbidae gave results of multiple alignment which showed that Columba livia livia and Columba eversmanni, closely resembled as well as Spilopelia senegalensis and Streptopelia decaocta. While Streptopelia tranquebarica and Spilopelia chinensis have great affinity due to small clade difference and Treron phoenicoptera was distinctly related to other species due to large clade difference.
{"title":"Molecular Taxonomy and Phylogenetic Analysis of Dove and Pigeon Species (Aves: Columbidae) of Pakistan, Based on COI Region of Mitochondrial DNA","authors":"Hina Rafiq, Fakhar -i-Abbas, Fakhra Nazir, F. M. Khan","doi":"10.35691/jbm.0202.0138","DOIUrl":"https://doi.org/10.35691/jbm.0202.0138","url":null,"abstract":"Doves and Pigeons are the members of living family Columbidae (Order: Columbiformes) having a wide range of taxonomic diversity and geographic distribution. Seven species with one sample each of family Columbidae were collected via random sampling from different districts of Pakistan to carry out this study. The targeted gene region was sequenced and identified by using BLAST tool at National Center for Biotechnology Information (NCBI). CLUSTALW was used for sequence alignment and MEGA6 for reconstruction of phylogenetic trees to predict the effective ancestry of different Columbidae species. The following phylogenetic trees were obtained i.e. Maximum Likelihood tree, Neighborhood joining tree, Maximum parsimony tree and UPGMA tree. In the current study, COI gene barcoding and phylogenetic analysis of family Columbidae gave results of multiple alignment which showed that Columba livia livia and Columba eversmanni, closely resembled as well as Spilopelia senegalensis and Streptopelia decaocta. While Streptopelia tranquebarica and Spilopelia chinensis have great affinity due to small clade difference and Treron phoenicoptera was distinctly related to other species due to large clade difference.","PeriodicalId":115980,"journal":{"name":"Journal of Bioresource Management","volume":"3 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2020-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"114566554","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A novel corona virus named as 2019 n-CoV was identified to be the actual cause of an outbreak of respiratory illness in Wuhan, China beginning in late December 2019. This respiratory disease was named as COVID-19 by WHO. There are many countries affected by COVID-19 including Pakistan. In this review we have provided a brief introduction of COVID-19 and discussed epidemiological and phylogenetic analysis of Pakistani novel corona virus strain. Our epidemiological analysis of data till 16 May 2020 showed rapidly increasing number of new cases, while at the same time a higher recovery rate than number of deaths in Pakistan. Moreover, phylogenetic analysis indicated that 2019-nCoV strain of Pakistan shared 100 % bootstrap value with various countries’ novel corona virus strains. Similarly, phylogenetic analysis was also conducted in comparison with SARS species to confirm our results. In this review, current knowledge of pathogenesis, diagnosis, treatment of COVID-19 and comorbidities which could be helpful in offering novel understanding and possible therapeutic targets for fighting against the COVID-19 infection are discussed.
{"title":"Current Scenario of Covid-19 with Epidemiological and Phylogenetic Analysis of Pakistani Coronavirus: A Review","authors":"A. Altaf, Wafa Iqbal, S. Younis, Farah Deebah","doi":"10.35691/jbm.0202.0139","DOIUrl":"https://doi.org/10.35691/jbm.0202.0139","url":null,"abstract":"A novel corona virus named as 2019 n-CoV was identified to be the actual cause of an outbreak of respiratory illness in Wuhan, China beginning in late December 2019. This respiratory disease was named as COVID-19 by WHO. There are many countries affected by COVID-19 including Pakistan. In this review we have provided a brief introduction of COVID-19 and discussed epidemiological and phylogenetic analysis of Pakistani novel corona virus strain. Our epidemiological analysis of data till 16 May 2020 showed rapidly increasing number of new cases, while at the same time a higher recovery rate than number of deaths in Pakistan. Moreover, phylogenetic analysis indicated that 2019-nCoV strain of Pakistan shared 100 % bootstrap value with various countries’ novel corona virus strains. Similarly, phylogenetic analysis was also conducted in comparison with SARS species to confirm our results. In this review, current knowledge of pathogenesis, diagnosis, treatment of COVID-19 and comorbidities which could be helpful in offering novel understanding and possible therapeutic targets for fighting against the COVID-19 infection are discussed.","PeriodicalId":115980,"journal":{"name":"Journal of Bioresource Management","volume":"41 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2020-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115994671","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
R. Raja, T. Z. Bokhari, Shakil Ahmad, S. Malik, K. Hussain, Kashif Nadeem
Wild plants have always held economic, nutritional and medicinal value for human beings. Present work is the study of local information of some wild plants being used for remedial purposes in District Muzaffarabad, Azad Jammu and Kashmir, Pakistan. The indigenous knowledge of local conventional uses was collected through survey and personal interviews during field trips. A total of 50 plant species were identified by taxonomic description using field guides and locally by medicinal knowledge of people living in the area. About 150 informers were interviewed randomly to record local names and ethno-medicinal uses of different plant species.
{"title":"Ethno-medicinal Survey for Some Wild Plants of Muzaffarabad, Azad Jammu & Kashmir, Pakistan","authors":"R. Raja, T. Z. Bokhari, Shakil Ahmad, S. Malik, K. Hussain, Kashif Nadeem","doi":"10.35691/jbm.0202.0136","DOIUrl":"https://doi.org/10.35691/jbm.0202.0136","url":null,"abstract":"Wild plants have always held economic, nutritional and medicinal value for human beings. Present work is the study of local information of some wild plants being used for remedial purposes in District Muzaffarabad, Azad Jammu and Kashmir, Pakistan. The indigenous knowledge of local conventional uses was collected through survey and personal interviews during field trips. A total of 50 plant species were identified by taxonomic description using field guides and locally by medicinal knowledge of people living in the area. About 150 informers were interviewed randomly to record local names and ethno-medicinal uses of different plant species.","PeriodicalId":115980,"journal":{"name":"Journal of Bioresource Management","volume":"69 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2020-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132919464","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M. Kamran, A. Yaqub, N. Malkani, K. M. Anjum, Muhammad Nabeel Awan, H. Paknejad
Channa are the freshwater and important food fish species in Pakistan belonging to family Channidae. However, identification and phylogenetic analysis based on molecular tools of these species in Pakistan was not well known. Herein, the current investigation was conceptualized, which dealt with mitochondrial DNA sequences from three geographically distinct populations of this species from Pakistan’s water system. DNA from fin tissues was extracted. COI region of mtDNA was amplified using universal primers for fish. PCR products were sequenced. Phylogenetic analysis conducted in the present study, i.e. neighbor-joining (NJ) cladogram, maximum likelihood, K2P genetic divergence and histogram suggests that the studied species of family Channidae are genetically different. The K2P intraspecific divergences were lower than interspecific divergences. The clades in the evolutionary tree for three species were clearly separated.
{"title":"Identification and Phylogenetic Analysis of Channa Species from Riverine System of Pakistan Using COI Gene as a DNA Barcoding Marker","authors":"M. Kamran, A. Yaqub, N. Malkani, K. M. Anjum, Muhammad Nabeel Awan, H. Paknejad","doi":"10.35691/jbm.0202.0135","DOIUrl":"https://doi.org/10.35691/jbm.0202.0135","url":null,"abstract":"Channa are the freshwater and important food fish species in Pakistan belonging to family Channidae. However, identification and phylogenetic analysis based on molecular tools of these species in Pakistan was not well known. Herein, the current investigation was conceptualized, which dealt with mitochondrial DNA sequences from three geographically distinct populations of this species from Pakistan’s water system. DNA from fin tissues was extracted. COI region of mtDNA was amplified using universal primers for fish. PCR products were sequenced. Phylogenetic analysis conducted in the present study, i.e. neighbor-joining (NJ) cladogram, maximum likelihood, K2P genetic divergence and histogram suggests that the studied species of family Channidae are genetically different. The K2P intraspecific divergences were lower than interspecific divergences. The clades in the evolutionary tree for three species were clearly separated.","PeriodicalId":115980,"journal":{"name":"Journal of Bioresource Management","volume":"12 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2020-07-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131126598","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
S. Hafeez, A. Mahmood, Rizwan Ahmed Khan, N. Malkani
Cancer is the second leading cause of death globally. However, in Pakistan, in the absence of a national cancer registry, it is difficult to predict the current status of cancer incidence. Therefore, a need was felt to design a study that can give a depiction of the prevalence of common cancer types and their relevance to the local population in the absence of a proper cancer registry system. In view of this, data was collected from 2010 to 2016 for breast, prostate, head and neck, cervical and colorectal cancer from the cancer hospitals and centres located all over Punjab, Pakistan. All the data were analysed to calculate prevalence percentage, gender-based incidence rate, crude rate, and Age-specific rate (ASR) for each cancer type. The results showed that breast cancer was the most common type and its prevalence showed a linear increase through the study period (P < 0.001). Breast cancer (6561) was followed by prostate (1183), head and neck (833), cervical (697) and colorectal cancer (531) in terms of prevalence. Gender-specific cancers like breast, prostate, and cervical were found to be more common as compared to others. In the case of head and neck and colorectal cancers, males were more susceptible as compared to females. There is a radical increase in cancer cases in the study area and the same could be extrapolated to the whole country. Therefore, for the appropriate and focused efforts to combat this increasing trend of prevalence, it should be constantly monitored, which leads to the recommendation of an effective cancer registry system in the country.
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