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Beating temporal phase sensitivity limit in off-axis interferometry based quantitative phase microscopy 基于离轴干涉的定量相位显微镜的跳动时间相位灵敏度限制
Pub Date : 2020-10-22 DOI: 10.1063/5.0034515
Yujie Nie, R. Zhou
Phase sensitivity determines the lowest optical path length (OPL) value that can be detected from the noise floor in a quantitative phase microscopy (QPM) system. The temporal phase sensitivity is known to be limited by both photon shot-noise and a variety of noise sources from electronic devices and environment. To beat temporal phase sensitivity limit, we explore different ways to reduce different noise factors in off-axis interferometry-based QPM using laser-illumination. Using a high electron-well-capacity camera, we measured the temporal phase sensitivity values using non-common-path and common-path interferometry based QPM systems under different environmental conditions. A frame summing method and a spatiotemporal filtering method are further used to reduce the noise contributions, thus enabling us to push the overall temporal phase sensitivity to less than 2 picometers.
相位灵敏度决定了在定量相位显微镜(QPM)系统中可以从噪声底检测到的最低光程长度(OPL)值。众所周知,时间相位灵敏度受到光子发射噪声和来自电子设备和环境的各种噪声源的限制。为了突破时间相位灵敏度的限制,我们探索了利用激光照明来降低离轴干涉QPM中各种噪声因素的不同方法。利用高电子阱容量相机,我们测量了不同环境条件下基于非共径和共径干涉测量的QPM系统的时间相位灵敏度值。我们进一步使用帧和方法和时空滤波方法来降低噪声贡献,从而使我们能够将整体时间相位灵敏度推至小于2皮米。
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引用次数: 12
A review of mass concentrations of Bramblings Fringilla montifringilla: implications for assessment of large numbers of birds 白杨的质量浓度综述:对大量鸟类评估的意义
Pub Date : 2020-10-16 DOI: 10.34080/OS.V31.22031
T. Svensson
Mass concentrations of birds, or lack of such, is a phenomenon of great ecological and domestic significance. Apart from being and indicator for e.g. food availability, ecological change and population size, it is also a source of conflict between humans and birds. Moreover, massive gatherings or colonies of birds also get the attention of the public -- either as a spectacular phenomenon or as an unwelcome pest -- thereby forming the public perception of birds and their abundance. In the context of the mass concentration of bramblings (Fringilla montifringilla) in Sweden the winter 2019-2020, this work reviews the literature on this striking phenomenon. Winter roosts are found to amount to on the order of one million birds per hectare of roost area, but the spread between reports is significant. Support for roosts of up to around 15 million birds was found, but much larger numbers are frequently recited in the literature. It is argued that these larger numbers are the result of overestimation or, in some cases, even completely unfounded (potentially typos). While the difficulties related to the count of large numbers of birds can explain this state, it is unfortunate that "high numbers" sometimes displace proper numbers. Since incorrect data, and its persistence, may result in that incorrect conclusions are drawn from new observations, this matter deserves attention. As the Brambling is a well-studied species, the matter also raises concerns regarding numbers for mass concentrations of other species. It is recommended that very large numbers of birds should be recited and used with care: underlying data and methods of the original sources should be scrutinized. Analogously, reporters of large numbers of birds are advised to describe and document counting methods. In particular, number estimates based on flock volume and bird density should be avoided.
鸟类的大量聚集或缺乏,是一种具有重大生态和家庭意义的现象。除了是食物供应、生态变化和人口规模等方面的指标外,它也是人类与鸟类之间冲突的根源。此外,大量聚集或成群的鸟类也引起了公众的注意——要么是作为一种壮观的现象,要么是作为一种不受欢迎的害虫——从而形成了公众对鸟类及其数量的认识。在2019-2020年冬季瑞典大量集中的树莓(Fringilla montifringilla)的背景下,本工作回顾了关于这一惊人现象的文献。据发现,冬季栖息的鸟类数量约为每公顷栖息面积100万只,但报告之间的差异很大。支持多达1500万只鸟的栖息地被发现,但在文献中经常引用更大的数字。有人认为,这些较大的数字是高估的结果,或者在某些情况下,甚至是完全没有根据的(潜在的打字错误)。虽然与大量鸟类计数有关的困难可以解释这种状态,但不幸的是,“高数量”有时取代了适当的数量。由于不正确的数据及其持续存在可能导致从新的观察中得出不正确的结论,因此这个问题值得注意。由于Brambling是一个被充分研究的物种,这个问题也引起了人们对其他物种质量浓度的关注。建议应谨慎地背诵和使用大量的鸟类:应仔细审查原始来源的基础数据和方法。类似地,建议大量鸟类的记者描述和记录计数方法。特别是,应避免根据鸟群数量和鸟类密度来估计数量。
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引用次数: 3
Spatial Registration Evaluation of [18F]-MK6240 PET [18F]-MK6240 PET的空间配准评价
Pub Date : 2020-10-15 DOI: 10.7916/D8-BF13-CY63
James Zou, Aubrey Johnson, Jeanelle France, S. Bharadwaj, Zeljko Tomljanovic, Y. Stern, A. Brickman, D. Devanand, J. Luchsinger, W. Kreisl, F. Provenzano
Image registration is an important preprocessing step in neuroimaging which allows for the matching of anatomical and functional information between modalities and subjects. This can be challenging if there are gross differences in image geometry or in signal intensity, such as in the case of some molecular PET radioligands, where control subjects display relative lack of signal relative to noise within intracranial regions, and may have off target binding that may be confused as other regions, and may vary depending on subject. The use of intermediary images or volumes have been shown to aide registration in such cases. To account for this phenomena within our own longitudinal aging cohort, we generated a population specific MRI and PET template from a broad distribution of 30 amyloid negative subjects. We then registered the PET image of each of these subjects, as well as a holdout set of thirty 'template-naive' subjects to their corresponding MRI images using the template image as an intermediate using three different sets of registration parameters and procedures. To evaluate the performance of both conventional registration and our method, we compared these to the registration of the attenuation CT (acquired at time of PET acquisition) to MRI as the reference. We then used our template to directly derive SUVR values without the use of MRI. We found that conventional registration was comparable to an existing CT based standard, and there was no significant difference in errors collectively amongst all methods tested. In addition, there were no significant differences between existing and MR-less tau PET quantification methods. We conclude that a template-based method is a feasible alternative to, or salvage for, direct registration and MR-less quantification; and, may be preferred in cases where there is doubt about the similarity between two image modalities.
图像配准是神经成像中一个重要的预处理步骤,它允许在模式和对象之间匹配解剖和功能信息。如果在图像几何形状或信号强度上存在明显差异,例如在一些分子PET放射配体的情况下,对照受试者显示相对于颅内区域的噪声相对缺乏信号,并且可能与其他区域混淆,并且可能因受试者而异。在这种情况下,使用中间图像或卷已被证明有助于注册。为了在我们自己的纵向老龄化队列中解释这一现象,我们从30个淀粉样蛋白阴性受试者的广泛分布中生成了一个特定人群的MRI和PET模板。然后,我们使用三套不同的配准参数和程序,将这些受试者的PET图像,以及一组由30名“未使用模板”的受试者组成的保留组与他们相应的MRI图像进行配准,使用模板图像作为中间物。为了评估常规配准和我们的方法的性能,我们将它们与作为参考的衰减CT(在PET采集时获得)与MRI的配准进行了比较。然后,我们使用我们的模板直接导出SUVR值,而不使用MRI。我们发现传统的配准与现有的基于CT的标准相当,所有测试方法的总体误差没有显著差异。此外,现有的tau PET定量方法与无mr的方法之间没有显著差异。我们得出结论,基于模板的方法是一种可行的替代方案,或挽救,直接登记和无磁共振量化;并且,在对两个图像模态之间的相似性有疑问的情况下,可以优选。
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引用次数: 0
Comparison of surface thermal patterns of horses and donkeys in IRT images 马和驴在红外热成像中的表面热模式比较
Pub Date : 2020-10-13 DOI: 10.5281/ZENODO.4085075
M. Domino, M. Romaszewski, Tomasz Jasi'nski, Malgorzata Ma'sko
Infrared thermography (IRT) is a valuable diagnostic tool in equine veterinary medicine however, little is known about its application in donkeys. The aim was to find patterns in thermal images of donkeys and horses, and determine if these patterns share similarities. The study was carried out on 18 donkeys and 16 horses. All equids underwent thermal imaging with an infrared camera and measuring the skin thickness and hair coat length. On the class maps of each thermal image, 15 regions of interest (ROIs) were annotated and then combined into 10 groups of ROIs (GORs). The existence of statistically significant differences between surface temperatures in GORs was tested both `globally' for all animals of a given species and `locally' for each animal. Two special cases of animals that differ from the rest were also discussed. Our results indicated that the majority of thermal patterns are similar for both species however, average surface temperatures in horses are higher than in donkeys. It may be related to differences in the skin and hair coat. We concluded, the patterns of both species are associated with GORs, rather than an individual ROI, with higher uniformity of donkeys patterns.
红外热像仪(IRT)是一种有价值的马兽医学诊断工具,但对其在驴上的应用知之甚少。目的是找出驴和马的热成像模式,并确定这些模式是否有相似之处。这项研究对18头驴和16匹马进行了研究。所有的马都接受了红外摄像机的热成像,并测量了皮肤厚度和毛发长度。在每个热图像的类图上,对15个感兴趣区域(roi)进行标注,然后将其合并为10组roi (GORs)。GORs中地表温度是否存在统计学上的显著差异,既对某一物种的所有动物进行了“全球”测试,也对每种动物进行了“局部”测试。还讨论了两种不同于其他动物的特殊情况。我们的研究结果表明,这两个物种的大多数热模式相似,但马的平均表面温度高于驴。这可能与皮肤和毛发的差异有关。我们得出结论,这两个物种的模式都与GORs相关,而不是单个ROI,驴模式的一致性更高。
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引用次数: 1
Utilization of 3D segmentation for measurement of pediatric brain tumor outcomes after treatment: review of available tools, step-by-step instructions, and applications to clinical practice 利用三维分割测量儿童脑肿瘤治疗后的结果:回顾可用的工具,逐步说明,并在临床实践中的应用
Pub Date : 2020-08-30 DOI: 10.26226/morressier.5e5e709500701ceba4a2695c
Marina Kazarian, Sandra A. Fadel, A. Mahajan, M. Aboian
Volumetric measurements are known to provide more information when it comes to segmenting tumors, in comparison to one- and two-dimensional measurements, and thus can lead to better informed therapy. In this work, we review the free and easily accessible computer platforms available for conducting these 3D measurements, such as Horos and 3D Slicer and compare the segmentations to commercial Visage software. We compare the time for 3D segmentation of tumors and demonstrate how to use a novel plugin that we developed in 3D slicer for the efficient and accurate segmentation of the cystic component of a tumor.
与一维和二维测量相比,体积测量在肿瘤分割方面提供了更多的信息,因此可以带来更好的治疗。在这项工作中,我们回顾了可用于进行这些3D测量的免费且易于访问的计算机平台,例如Horos和3D切片器,并将分割与商业Visage软件进行比较。我们比较了肿瘤的3D分割时间,并演示了如何使用我们在3D切片机中开发的新颖插件,以高效准确地分割肿瘤的囊性成分。
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引用次数: 0
Diagnostic accuracy of computed tomography for identifying hospitalizations for patients with COVID-19 计算机断层扫描识别COVID-19患者住院情况的诊断准确性
Pub Date : 2020-07-29 DOI: 10.17816/DD46818
S. Morozov, R. Reshetnikov, Victor A. Gombolevskiy, N. Ledikhova, I. Blokhin, V. Kljashtorny, O. Mokienko, A. Vladzymyrskyy
The controversy of computed tomography (CT) use in COVID-19 screening is associated with ambiguous characteristics of chest CT as a diagnostic test. The reported values of CT sensitivity and specificity calculated using RT-PCR as a reference standard vary widely. The objective of this study was to reevaluate the diagnostic and prognostic value of CT using an alternative approach. This study included 973 symptomatic COVID-19 patients aged 42 $pm$ 17 years, 56% females. We reviewed the disease dynamics between the initial and follow-up CT studies using a "CT0-4" grading system. Sensitivity and specificity were calculated as conditional probabilities that a patient's condition would improve or deteriorate relative to the initial CT study results. For the calculation of negative (NPV) and positive (PPV) predictive values, we estimated the COVID-19 prevalence in Moscow. We used several ARIMA and EST models with different parameters to fit the data on total cases of COVID-19 from March 6, 2020, to July 20, 2020, and forecast the incidence. The "CT0-4" grading scale demonstrated low sensitivity (28%) but high specificity (95%). The best statistical model for describing the pandemic in Moscow was ETS with multiplicative trend, error, and season type. According to our calculations, with the predicted prevalence of 2.1%, the values of NPV and PPV would be 98% and 10%, correspondingly. We associate the low sensitivity and PPV values with the small sample size of the patients with severe symptoms and non-optimal methodological setup for measuring these specific characteristics. The "CT0-4" grading scale was highly specific and predictive for identifying admissions to hospitals of COVID-19 patients. Despite the ambiguous accuracy, chest CT proved to be an effective practical tool for patient management during the pandemic, provided that the necessary infrastructure and human resources are available.
计算机断层扫描(CT)用于COVID-19筛查的争议与胸部CT作为诊断测试的模糊特征有关。以RT-PCR作为参考标准计算的CT敏感性和特异性的报道值差异很大。本研究的目的是重新评估CT的诊断和预后价值,采用另一种方法。本研究纳入973例有症状的COVID-19患者,年龄42岁,年龄17岁,56%为女性。我们使用“CT0-4”分级系统回顾了初始和随访CT研究之间的疾病动态。敏感性和特异性被计算为患者病情相对于初始CT研究结果改善或恶化的条件概率。为了计算阴性(NPV)和阳性(PPV)预测值,我们估计了莫斯科的COVID-19流行率。采用不同参数的ARIMA和EST模型,拟合2020年3月6日至2020年7月20日新冠肺炎总病例数据,并进行发病预测。CT0-4分级表敏感性低(28%),特异性高(95%)。描述莫斯科大流行的最佳统计模型是具有乘法趋势、误差和季节类型的ETS。根据我们的计算,在预测患病率为2.1%时,NPV和PPV的值分别为98%和10%。我们将低灵敏度和PPV值与严重症状患者的小样本量以及测量这些特定特征的非最佳方法设置联系起来。“CT0-4”分级量表对识别COVID-19住院患者具有高度的特异性和预测性。尽管准确性不明确,但在具备必要的基础设施和人力资源的情况下,胸部CT被证明是大流行期间患者管理的有效实用工具。
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引用次数: 2
Effects of Bacterial Density on Growth Rate and Characteristics of Microbial-Induced CaCO3 Precipitates: Particle-Scale Experimental Study 细菌密度对微生物诱导CaCO3沉淀生长速率和特性的影响:颗粒尺度实验研究
Pub Date : 2020-06-29 DOI: 10.1061/(ASCE)GT.1943-5606.0002509
Yuze Wang, K. Soga, J. DeJong, A. Kabla
Microbial-Induced Carbonate Precipitation (MICP) has been explored for more than a decade as a promising soil improvement technique. However, it is still challenging to predict and control the growth rate and characteristics of CaCO3 precipitates, which directly affect the engineering performance of MICP-treated soils. In this study, we employ a microfluidics-based pore scale model to observe the effect of bacterial density on the growth rate and characteristics of CaCO3 precipitates during MICP processes occurring at the sand particle scale. Results show that the precipitation rate of CaCO3 increases with bacterial density in the range between 0.6e8 and 5.2e8 cells/ml. Bacterial density also affects both the size and number of CaCO3 crystals. A low bacterial density of 0.6e8 cells/ml produced 1.1e6 crystals/ml with an average crystal volume of 8,000 um3, whereas a high bacterial density of 5.2e8 cells/ml resulted in more crystals (2.0e7 crystals/ml) but with a smaller average crystal volume of 450 um3. The produced CaCO3 crystals were stable when the bacterial density was 0.6e8 cells/ml. When the bacterial density was 4-10 times higher, the crystals were first unstable and then transformed into more stable CaCO3 crystals. This suggests that bacterial density should be an important consideration in the design of MICP protocols.
微生物诱导碳酸盐降水(MICP)作为一种很有前途的土壤改良技术已经被探索了十多年。然而,预测和控制CaCO3沉淀的生长速度和特征仍然是一个挑战,这直接影响了micp处理后土壤的工程性能。在本研究中,我们采用基于微流体的孔隙尺度模型来观察细菌密度对发生在砂粒尺度上的MICP过程中CaCO3沉淀物生长速率和特征的影响。结果表明:CaCO3的沉淀速率随菌密度的增大而增大,在0.68 ~ 5.28个细胞/ml范围内;细菌密度也影响CaCO3晶体的大小和数量。低细菌密度为0.68个细胞/ml产生1.1e6个晶体/ml,平均晶体体积为8,000 um3,而高细菌密度为5.28个细胞/ml产生更多晶体(2.0e7个晶体/ml),但平均晶体体积较小,为450 um3。当细菌密度为0.68个细胞/ml时,CaCO3晶体稳定。当细菌密度增加4-10倍时,晶体首先不稳定,然后转变为更稳定的CaCO3晶体。这表明细菌密度应该是设计MICP协议的重要考虑因素。
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引用次数: 38
A Semiempirical Dynamical Model to Forecast the Propagation of Epidemics: The Case of the Sars-Cov-2 in Spain 预测流行病传播的半经验动力学模型——以西班牙Sars-Cov-2为例
Pub Date : 2020-04-19 DOI: 10.1101/2020.04.19.20071860
J. Mora, Sandra P'erez, I. Rodr'iguez, A. N'unez, A. Dvorzhak
A semiempirical model, based in the logistic map, has been succesfully applied to forecast important quantities along the several phases of the outbreak of the covid-19 for different countries. This paper shows how the model was calibrated and applied to perform predictions of people needing to be hospitalized, needs of ventilators, or the number of deaths which would be produced. It is shown specifically the results obtained in the case of Spain, showing a prediction of diagnosed infected and deaths which will be observed after the ease of the total lockdown produced the 13th of March. Is also shown how this model can provide an insight of what the level of infection in the different regions of Spain is forecasted. The model predicts for Spain for the end of May more than 400,000 diagnosed infected cases, number which will be probably higher due to the change in the possibilities of performing massive number of tests to the general population. The number of forecasted deaths for that date is 46,000 +/- 15,000. The model also predicts the level of infection at the different Spanish regions, providing a counterintuitive result in the cases of Madrid and Catalonia as the result shows a higher the level of infection at Catalonia than the level at Madrid, according with this model. All of these results can be used to guide policy makers in order to optimize resources and to avoid future outbreaks of the covid-19.
基于logistic图的半经验模型已成功应用于预测不同国家2019冠状病毒病爆发的几个阶段的重要数量。本文展示了如何校准模型并应用于预测需要住院的人、呼吸机的需求或将产生的死亡人数。其中具体显示了在西班牙获得的结果,显示了在3月13日全面封锁放松后将观察到的诊断感染和死亡人数的预测。还显示了该模型如何提供西班牙不同地区感染水平的预测。该模型预测,到5月底,西班牙确诊的感染病例将超过40万例,由于对一般人群进行大量检测的可能性发生变化,这一数字可能会更高。该日期的预测死亡人数为46,000 +/- 15,000。该模型还预测了西班牙不同地区的感染水平,在马德里和加泰罗尼亚的情况下提供了一个违反直觉的结果,因为根据该模型,加泰罗尼亚的感染水平高于马德里的水平。所有这些结果都可用于指导决策者优化资源并避免未来爆发covid-19。
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引用次数: 3
Parameter selection methods in inverse problem formulation 反问题公式中的参数选择方法
Pub Date : 2020-04-15 DOI: 10.1007/978-3-642-32882-4_3
H. Banks, A. Cintrón-Arias, A. Cintrón-Arias, F. Kappel, F. Kappel
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引用次数: 41
Theoretical and Numerical Considerations of the Assumptions Behind Triple Closures in Epidemic Models on Networks 网络上流行病模型三重闭包假设的理论和数值考虑
Pub Date : 2019-09-02 DOI: 10.1007/978-3-030-46306-9_14
N. Georgiou, I. Kiss, P. Simon
{"title":"Theoretical and Numerical Considerations of the Assumptions Behind Triple Closures in Epidemic Models on Networks","authors":"N. Georgiou, I. Kiss, P. Simon","doi":"10.1007/978-3-030-46306-9_14","DOIUrl":"https://doi.org/10.1007/978-3-030-46306-9_14","url":null,"abstract":"","PeriodicalId":119149,"journal":{"name":"arXiv: Quantitative Methods","volume":"20 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121553819","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
期刊
arXiv: Quantitative Methods
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