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The Relationship between Diazinon and Malathion Plasma Levels with Follicular Cyst Disease in Dairy Cows 奶牛血浆二嗪农和马拉硫磷水平与卵泡囊肿病的关系
Pub Date : 2017-05-26 DOI: 10.15412/J.JBTW.01060503
A. Sayad, M. Abdollahi, M. Vodjgani, K. Abdi, F. Gharagozloo, M. Rezvanfar, H. Akbarein, M. Koohi
Ovarian cyst is a common disease affecting dairy cows. It is one of the major causes of infertility in dairy cattle. Pathological ovarian cysts are classified into two groups including follicular and luteal cysts. Due to reduced fertility, increased calving interval, and the increase in the culling of cattle as a result of infertility, livestock industry has suffered from abundant financial losses. Hormonal and endocrine disorders are the causes of this disease that may occur through endocrine disrupting pesticides. This study aimed to investigate the relationship between plasma levels of diazinon and malathion with follicular cysts in dairy cattle. A case-control study was conducted on 30 cows. Diagnosis and selection of 15 cows (as the case group) with follicular cysts were done via rectal touch and ultrasonography, and plasma progesterone level was measured by a specialist. Control group consisted of 15 healthy cows that were homogenized with case group. Blood samples were taken from tail vein and were centrifuged, and the plasma was separated and stored at -80° C. Malathion and diazinon pesticides were measured by GC-MS (Gas Chromatography Mass) method in the plasma of the samples with 30ppb resolution. Independent t-test was used for comparing the means in healthy and case independent groups. P 0.05), it can be stated that there is no correlation between diazinon and malathion pesticides, and the risk of follicular cysts in dairy cows.
卵巢囊肿是奶牛的常见病。这是奶牛不育的主要原因之一。病理性卵巢囊肿分为卵泡囊肿和黄体囊肿两类。由于生育力下降,产犊间隔增加,以及由于不孕症导致的牛的扑杀增加,畜牧业遭受了巨大的经济损失。激素和内分泌紊乱是该病的病因,可能通过干扰内分泌的农药发生。本研究旨在探讨奶牛血浆中二嗪农和马拉硫磷水平与卵泡囊肿的关系。对30头奶牛进行病例对照研究。选取15头患有卵泡囊肿的奶牛作为病例组,通过直肠触摸和超声检查进行诊断和选择,并由专家检测血浆孕酮水平。对照组15头健康奶牛,与病例组均质。取尾静脉血样离心,分离血浆-80℃保存,样品血浆中马拉硫磷和二嗪农农药用GC-MS(气相色谱质谱)法测定,分辨率30ppb。采用独立t检验比较健康组和病例独立组的均值。P < 0.05),说明二嗪农和马拉硫磷农药与奶牛卵泡囊肿风险无相关性。
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引用次数: 0
Fluorescent Diagnosis of Bladder Cancer by Hexasens as a Drug Hexasens作为药物的荧光诊断膀胱癌
Pub Date : 2017-05-24 DOI: 10.15412/J.JBTW.01060701
Slovokhodov Egor Konstantinovich, Ivanova-Radkevich Veronika Igorevna, Brodsky Ilya Borisovich
This study was performed as a clinical trial conducted in Russia concerning the diagnostic efficacy of Hexasens drug. The photosensitizer based on hexyl ester of 5-aminolevulinic acid was used for the fluorescence diagnosis of bladder cancer. The study was conducted in 2015-2016 on the basis of the City Clinical Hospital No. 40 (Moscow, Russia). 53 patients were involved into study and were diagnosed as bladder cancer in T1N0M0 stage. Fluorescence diagnosis was carried out for 1 hour after the intravesical induction of 0.2% solution (50 mL or 100 mg) of Hexasens drug (FSUE "SSC NIOPIK", Russia). The results of the fluorescence diagnosis were compared with the results of the standard routine cystoscopy. The found results showed that the performance of fluorescent diagnostics (FD) led to improve diagnostic sensitivity as 13.2% compared to standard cystoscopy (from 86.8% to 100%); the diagnosis accuracy increased as 8.8% (from 91.2% to 100%) and a negative predictive value increased as 20.9% (from 79.1% to 100%). During the carrying out of fluorescence diagnosis, 8 patients (15.0%) out of 53 ones also revealed 14 fluorescing foci that not identified in white light, in which the tumorous process in them was confirmed during morphological study. The induction of Hexasens solution into a bladder and the carrying out of a fluorescent diagnosis was not accompanied by the development of any adverse reactions. Patients showed a subjective discomfort only during a prolonged exposure of the drug solution in a bladder (up to 2 hours). The developed diagnosis methods is recommended for the applying in advanced clinical diagnosis.
本研究是在俄罗斯进行的一项关于Hexasens药物诊断疗效的临床试验。以5-氨基乙酰丙酸己基酯为基础的光敏剂用于膀胱癌的荧光诊断。该研究于2015-2016年在莫斯科第40市临床医院(俄罗斯)进行。53例膀胱癌患者被诊断为T1N0M0期。用0.2% Hexasens药物溶液(50 mL或100 mg) (FSUE“SSC NIOPIK”,俄罗斯)膀胱诱导1小时后进行荧光诊断。荧光诊断结果与标准常规膀胱镜检查结果进行比较。结果表明,与标准膀胱镜检查相比,荧光诊断(FD)的诊断灵敏度提高了13.2%(从86.8%提高到100%);诊断正确率提高了8.8%(从91.2%提高到100%),阴性预测值提高了20.9%(从79.1%提高到100%)。在进行荧光诊断时,53例患者中有8例(15.0%)还发现了14个白光下未发现的荧光灶,形态学研究证实了其肿瘤进程。将Hexasens溶液导入膀胱并进行荧光诊断,未出现任何不良反应。患者仅在药物溶液长期暴露于膀胱(长达2小时)时才表现出主观不适。所建立的诊断方法可用于临床高级诊断。
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引用次数: 2
Development of a Denitrifying Biofilter with Short Start-up Period 短启动周期反硝化生物滤池的研制
Pub Date : 2017-05-22 DOI: 10.15412/J.JBTW.01060403
Alireza Oboodi, E. Fathi, H. Nezammahalleh
In the present work, a novel down-flow biofilter with four identical layers was developed for the de-nitrification of 100 mg L-1 nitrate contaminated wastewater, flowing horizontally from one layer to another in a continuous fashion. This biofilter can be applied for long-term continuous wastewater treatment without any need to whole system shutdown and backwashing. The clogged layer or the deactivated section can be easily spotted and repaired while the system is under continuous operation. To attain a short startup period, the effect of two main constructional parameters, namely, filter media and inoculants source, were then investigated.. Two studied filter media were sand and woodchips. The pure culture of Saccharomyces cerevisiae and the mixed cultures of activated sludge and wastewater were three common inoculants under investigation. The biofilter packed with woodchips seeded with the wastewater resulted in the least startup period of about 3 days with a steady nitrate removal efficiency of 90 %.
在本工作中,开发了一种新型的四层下流式生物过滤器,用于100 mg L-1硝酸盐污染废水的脱氮,以连续的方式从一层水平流动到另一层。该生物滤池可长期连续处理废水,不需要整个系统停机和反冲洗。在系统连续运行时,可以很容易地发现和修复堵塞层或停用段。为了获得较短的启动时间,研究了两个主要结构参数,即过滤介质和孕育剂来源对启动时间的影响。研究的两种过滤介质是沙子和木屑。酿酒酵母菌纯培养物和活性污泥与废水混合培养物是目前研究的三种常见的接种剂。用木屑填充生物滤池后,废水的启动时间最短,约为3天,硝酸盐去除率稳定在90%左右。
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引用次数: 1
Clinical-diagnostic Significance of Recognition of Specific Antibodies in Patients with Allergies to Metal Alloys Used in Prosthetic Dentistry 牙体修复用金属合金过敏患者特异性抗体识别的临床诊断意义
Pub Date : 2017-05-15 DOI: 10.15412/J.JBTW.01060501
E. S. Mikhailova, Vladimir Valerevich Кostyunichev, L. Ermolaeva, Natalia Alexandrovna Socolovich, N. A. Ogrina, N. A. Sheveleva, Sergey Nikolaevich Zhovtyy, A. A. Polens
A comprehensive survey of 226 patients with an allergy to metal alloys applied in the clinic prosthodontics. In this study, the total Immunoglobulin E (IgE), specific IgE- and Immunoglobulin G (IgG) -antibodies to metal ions in serum and in mixed saliva were measured. In the mixed saliva no significant increasing in IgE-antibodies to metal ions has been revealed, IgG-antibodies to gold, cobalt, chromium, nickel, copper, palladium and platinum ions in low titers were defined in elderly patients. To predict the development of allergic reactions in patients with metal alloys prosthetic constructions, specific serum IgG-antibodies to metal ions that characterize the frequency of contact with the allergen were measured. The content of specific IgE-antibodies to metal ions in serum measured to recognize a primary response to allergens (no history of contact with the metal alloys used in prosthetic dentistry). The investigation of specific IgE- and IgG-antibodies to metal ions in serum has shown the largest number of positive tests to copper (11.3%), gold (10.8%), cobalt (9.9%) and nickel ions (9.3%). The verification of clinical and diagnostic significance of revealing specific antibodies to metal ions in patients with allergies to metal alloys used in prosthetic dentistry, showed that the use of these methods is an auxiliary diagnostic tool and it can only be interpreted in conjunction with the results of clinical examination of the patients.
临床修复中对金属合金过敏226例的综合调查。本研究测定了血清和混合唾液中对金属离子的总免疫球蛋白E (IgE)、特异性IgE和免疫球蛋白G (IgG)抗体。老年患者混合唾液中对金属离子的ige抗体未见明显升高,对金、钴、铬、镍、铜、钯、铂离子的igg抗体呈低滴度。为了预测金属合金假体结构患者过敏反应的发展,测量了与过敏原接触频率表征的金属离子特异性血清igg抗体。测定血清中针对金属离子的特异性ige抗体的含量,以识别对过敏原的主要反应(没有接触牙科假体中使用的金属合金的历史)。血清中针对金属离子的特异性IgE和igg抗体的调查显示,铜离子(11.3%)、金离子(10.8%)、钴离子(9.9%)和镍离子(9.3%)的阳性试验最多。对金属合金过敏患者金属离子特异性抗体的临床诊断意义的验证,表明这些方法的使用是一种辅助诊断工具,只能结合患者的临床检查结果来解释。
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引用次数: 1
Heavy Metal Removal by Phosphate Solubilizing Acinetobacter calcoaceticus Isolated from Rhizosphere 根际钙酸不动杆菌磷酸盐增溶去除重金属的研究
Pub Date : 2017-05-10 DOI: 10.15412/J.JBTW.01061104
F. Ghoreishi, Z. Etemadifar
Worldwide industrial activities produce large amount of metal wastes. Metals remediation by microbial activities associated with phosphorus metabolism is a promising method. The goals of this study were the removal of some heavy metals by isolated phosphate solubilizing rhizobacterium (PSR) and survey the effect of total secreted materials including extracellular phosphatase on this phenomenon. PSR strain was isolated by Pikovskaya (PVK) medium and identified by 16S rDNA sequencing. Its phosphatase enzyme was assayed by colorimetric method. Metal resistance of the isolate was measured by the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC). The metal removal was detected by atomic absorption spectroscopy. The culture supernatant was analyzed for metal removal by secreted phosphatase enzyme with or without tri-calcium phosphate (TCP), as its substrate. The isolated rhizobacterium was identified as Acinetobacter calcoaceticus that produced acid phosphatase enzyme. The MICs50 were obtained 2.5 mM Pb(II), 2 mM Ni(II), 0.2 mM Cr(II) and E‚1 mM Cd(II); and the MBCs were >100 mM Ni(II), 40 mM Cd(II), 20 mM Pb(II), and 1 mM (Cr(II). The removal of metals by the cells were obtained 29.45% Cr(II), 25.74% Pb(II), 18.85% Cd(II) and 11.43% Ni(II). It was not seen any nickel removal by microbial secreted products in PVK medium without TCP, whereas removal was obtained in PVK complimented by TCP 28.3%; lead removal was almost the same in both media (99%) and was not related to TCP.
世界范围内的工业活动产生大量的金属废料。利用磷代谢相关的微生物活性修复金属是一种很有前途的方法。本研究的目的是利用分离的磷酸溶根细菌(PSR)去除某些重金属,并探讨包括胞外磷酸酶在内的总分泌物质对这一现象的影响。采用Pikovskaya (PVK)培养基分离PSR菌株,并进行16S rDNA测序鉴定。用比色法测定其磷酸酶。采用最小抑菌浓度(MIC)和最小杀菌浓度(MBC)测定菌株对金属的抗性。用原子吸收光谱法检测金属的去除率。用分泌的磷酸酶对培养上清进行除金属分析,以三磷酸钙(TCP)为底物或不以三磷酸钙(TCP)为底物。分离得到的根杆菌鉴定为产酸性磷酸酶的钙酸不动杆菌。MICs50分别为2.5 mM Pb(II)、2 mM Ni(II)、0.2 mM Cr(II)和1 mM Cd(II);MBCs >100 mM Ni(II)、40 mM Cd(II)、20 mM Pb(II)、1 mM Cr(II)。电池对金属的去除率分别为29.45% Cr(II)、25.74% Pb(II)、18.85% Cd(II)和11.43% Ni(II)。在不添加TCP的PVK培养基中,微生物分泌产物对镍没有去除效果,而在添加了28.3% TCP的PVK培养基中,微生物分泌产物对镍有去除效果;两种介质的铅去除率几乎相同(99%),与TCP无关。
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引用次数: 2
Investigating the Relationship between rs7903146 Polymorphism Genotypes of TCF7L2 Gene and Visfatin Serum Level in Patients with Type 2 Diabetes 2型糖尿病患者TCF7L2基因rs7903146多态性基因型与血清Visfatin水平的关系研究
Pub Date : 2017-04-30 DOI: 10.15412/J.JBTW.01060402
Zahra Asemi-Esfahani, F. Alian, M. Fazilati, H. Nazem, A. Palizban
This study showed that T allele is a risk allele for both homozygous and heterozygous in rs7903146 polymorphism genotyping in TCF7L2 gene. Here, 132 individuals were investigated (87 of them were patients with type 2 diabetes and 45 other were healthy, respectively). DNA was extracted from whole blood samples via a purification kit; then in order to the determination of quality, it was electrophoresised on agarose gels. Genotyping method was performed through high-resolution melt (HRM) reverse transcription polymerase chain reaction (RT-PCR). The visfatin level was determined based on enzyme-linked immunosorbent assay (ELISA) method. According to the achieved results, increasing the plasma levels of visfatin in diabetics patients (10.73 ± 5.38) compared to the control group (6.73 ± 1.88) and their changes based on the separation of genotypes also confirms that T allele is the probable risk factor (p-value < 0.05). In addition, the presence of the T allele is related to increasing triglyceride (TG) levels of blood. Therefore, adipose tissue burning and its reduction (along with the releasing of TG in the blood) decreased the source of visfatin, therefore increasing the TG in the presence of T allele (the development of diabetes) is associated with decreased plasma levels of visfatin. According to this study, evaluating plasma levels of visfatin and TG levels in blood is useful in prognosis of type 2 diabetes.
本研究表明,T等位基因是TCF7L2基因rs7903146多态性基因分型中纯合和杂合的危险等位基因。在这里,研究人员调查了132人(其中87人是2型糖尿病患者,另外45人是健康的)。通过纯化试剂盒从全血样本中提取DNA;然后在琼脂糖凝胶上进行电泳,以确定其质量。采用高分辨率熔融(HRM)逆转录聚合酶链反应(RT-PCR)进行基因分型。采用酶联免疫吸附法(ELISA)测定内脏脂肪素水平。结果显示,糖尿病患者血浆visfatin水平(10.73±5.38)高于对照组(6.73±1.88),且其基于基因型分离的变化也证实了T等位基因是可能的危险因素(p值< 0.05)。此外,T等位基因的存在与血液中甘油三酯(TG)水平的升高有关。因此,脂肪组织燃烧及其减少(伴随着血液中TG的释放)减少了内脏脂肪素的来源,因此,在T等位基因存在的情况下增加TG(糖尿病的发展)与血浆内脏脂肪素水平降低有关。根据本研究,评估血浆中visfatin水平和血液中TG水平对2型糖尿病的预后有帮助。
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引用次数: 2
Analyzing the Efficacy of Fuzzy logic in the Concentration of Cadmium in Soil and Potatoes (Case Study in Absard City of Damavand) 模糊逻辑在土壤和马铃薯镉浓度监测中的效果分析(以达马万市为例)
Pub Date : 2017-04-30 DOI: 10.15412/J.JBTW.01060401
Maryam Valadkhani, Shahrzad Khoramnejadian, H. Radnezhad
Owing to complexity, uncertainty in natural phenomena and natural dynamism, Fuzzy logic puts the obscurities in mathematical formation and preparation for decision-making and reasoning. The present analysis represents the accuracy of Fuzzy interpretation (qualitative measurement of cadmium) in estimating the concentration of cadmium in soil and the potato product in 8 pieces of farmland in Absard, Damavand. Some physical and chemical features of soil, together with the amount of cadmium concentrated in soil and potatoes, were measured three times by Flame and Graphite Furnace Atomic Absorption Spectrometer and were analyzed through Mamdani Inference System by Toolbox of Matlab. The cadmium in potatoes and roadside farmlands was measured to be more than the standard amount; therefore, it illustrates high cadmium toxicity in the mentioned area. It is concluded that Fuzzy logic (the rule of if-then) has a more appropriate and accurate approach to evaluating the concentration of cadmium in soil and product regarding the attention that is paid to the impact of all the characteristics of soil and the complex relationship among the parameters and plant type.
由于自然现象的复杂性、不确定性和自然动态性,模糊逻辑为决策和推理的数学形成和准备提供了模糊性。本文分析了模糊解释法(定性镉测量法)在估计达马万省阿布萨特8块农田土壤和马铃薯产品中镉含量中的准确性。利用火焰原子吸收光谱仪和石墨炉原子吸收光谱仪三次测定了土壤的一些理化特征以及土壤和马铃薯中镉的富集量,并通过Matlab工具箱中的Mamdani推理系统进行了分析。马铃薯和路边农田镉含量超标;因此,它说明了该地区镉的高毒性。结果表明,模糊逻辑(if-then规则)由于考虑了土壤各特性的影响以及参数与植物类型之间的复杂关系,是评价土壤和产品中镉浓度更为合适和准确的方法。
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引用次数: 0
Surveying the Rate of Using Cosmetics Among the Kashan's Women 卡尚妇女化妆品使用率调查
Pub Date : 2017-04-03 DOI: 10.15412/J.JBTW.01060202
R. Dehghani, R. Talaee, M. Sehat, Narges Nikkhah Ghamsari, L. Mesgari
Using cosmetics is very popular among women in Iran. Direct contact with some of the cosmetics can cause irreparable harm to consumers’ health Regarding the importance of the rate of using cosmetics and the possibility of their side effects, this research studies the rate of using cosmetics among the Kashan's women. Data of this study were obtained through a designed questionnaire filled by 800 women in Kashan. Sampling was done randomly and in a multistage cluster. After obtaining the data, the graphs and tables were drawn and the data were analyzed by Chi-square and Mann–Whitney. The highest rate of continuous use of cosmetics was in the 20-29 years old group and the lowest was in the age group of 49 years old and above. The highest rate of continuous use of cosmetics was in the women with master’s degree, above (47.6%) and the lowest was among the illiterates (10%). 50% of People with high income and 34.8% with lower income used cosmetics continuously. Among the cosmetics, the most used item was lipstick with 74.5%.Variables such as demographic characteristics, socioeconomic status, family relations are the effective factors on the rate of using cosmetics by women .The main reason for using cosmetics by women was enhancing beauty and attracting attention.
使用化妆品在伊朗女性中非常流行。直接接触某些化妆品会对消费者的健康造成不可弥补的伤害。关于化妆品使用率的重要性及其副作用的可能性,本研究研究了喀山妇女的化妆品使用率。本研究的数据是通过一份设计好的问卷获得的,问卷由800名喀山妇女填写。抽样是随机进行的,并在一个多阶段的集群。获得数据后,绘制图表,并采用卡方法和Mann-Whitney法对数据进行分析。持续使用化妆品的比例以20 ~ 29岁年龄组最高,49岁及以上年龄组最低。持续使用化妆品的比例以硕士以上女性最高(47.6%),文盲最低(10%)。50%的高收入人群和34.8%的低收入人群持续使用化妆品。化妆品中使用最多的是口红,占74.5%。人口特征、社会经济地位、家庭关系等变量是影响女性化妆品使用率的有效因素,女性使用化妆品的主要原因是美容和吸引眼球。
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引用次数: 7
Solpugidophobia in Iran: Real or illusion 伊朗的溶洞恐惧症:是真还是假
Pub Date : 2017-03-22 DOI: 10.15412/J.JBTW.01060302
R. Dehghani
Many Many reports have shown the fear of Solpugids in Iran. Solpugids are not poisonous, but because of morphological characteristics and behaviors, have caused panic in people. Large portion of this fear is unreal; because most of the people are not aware of the details of these animals life. Another part of phobia may be related to psychological or mental characteristics of people. Therefore, paying attention to this issue has a special importance.
许多报道都显示了伊朗对索帕吉德的恐惧。溶洞虫是无毒的,但由于其形态特征和行为,引起了人们的恐慌。这种恐惧很大一部分是不真实的;因为大多数人都不知道这些动物生活的细节。恐惧症的另一部分可能与人的心理或精神特征有关。因此,关注这个问题具有特殊的重要性。
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引用次数: 11
Behavioral Defensive Mechanism in Infertile Couples 不育夫妇的行为防御机制
Pub Date : 2017-03-15 DOI: 10.15412/J.JBTW.01060303
B. Rashidi, H. Afzali, F. Haghollahi, M. Abedini, M. Eslami, H. K. Jaliseh
Precise information related to behavioral patterns of infertile couples are limited. Therefore, the aim of this study is the assessing the behavioral issues in infertile couples in Iran. In this cross-sectional study, 680 individuals (infertile couples who married at least one year and received primary or secondary infertility diagnosis) who referred to five public and seven private and semi-private centers of infertility across the country were assessed by using non-probability sampling of available individuals. Data collection tool is researcher-made questionnaires to determine the defense mechanism of individuals to cope with infertility and consisted of 21 questions with five Likert scale. Standard score range was from zero to 100 which higher score was developed defense mechanism. Average age in all provinces was generally 30 years (20-40 years) for women and 34 years (22-50 years) for men. A total of 325(48.1%) of women and 419 (65.7%) of men had developed a mature defense mechanism and men were slightly more developed than women in defense mechanism.(P<0.001). Regression coefficients analysis showed the most important factor influencing man’s defense mechanism score is his educational level.(p=0.008). Increased men's education lead to increased mature defense mechanism score in infertile individuals and will make this attitude more developed.
有关不孕夫妇行为模式的准确信息是有限的。因此,本研究的目的是评估伊朗不孕夫妇的行为问题。在这项横断面研究中,通过对可用个体的非概率抽样,对680名个体(结婚至少一年并接受原发性或继发性不孕症诊断的不育夫妇)进行了评估,这些个体分别来自全国5个公立和7个私立和半私立不孕症中心。数据收集工具为研究者自行制作问卷,确定个体应对不孕症的防御机制,共21个问题,采用5个李克特量表。标准得分范围为0 ~ 100分,得分越高,防御机制越发达。所有省份的平均年龄一般为妇女30岁(20-40岁),男子34岁(22-50岁)。女性325人(48.1%),男性419人(65.7%),防卫机制发育成熟,男性略高于女性(P<0.001)。回归系数分析显示,影响男子防卫机制得分的最主要因素是文化程度(p=0.008)。男性受教育程度的提高导致不育个体成熟防御机制得分的提高,并将使这种态度更加发达。
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引用次数: 3
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