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Impact of Profenofos and Carbosulfan on Dehydrogenase activity of Freshwater fish, Labeo rohita (Hamilton) 异丙磷和硫丹对淡水鱼脱氢酶活性的影响
Pub Date : 2017-01-22 DOI: 10.15412/J.JBTW.01060103
Nagaraju Bantu, Z. Hagos, Chaitanya Krishna, G. Krishnan, Rathnamma Vakita, Suneetha Karimasetty, Nirmala Kumari Bantu
Experiments were intended to study effects of profenofos and carbosulfan on the activities of dehydrogenase of freshwater fish, Labeo rohita. Healthy freshwater fish, Labeo rohita (Hamilton) were collected from the Nandivelugu fishfarm, India. Profenofos and carbosulfan pesticides were purchased from local pesticide store in Guntur, India, based on the preliminary lethal toxicity tests (96 hr LC50 i.e. 100 μg l-1; 1.2 mg l-1 and 1/10th 96 hr LC50 i.e.10 μg l-1; 0.12mg l-1 and) were selected as lethal and sublethal concentrations. After the acclimation, fish were exposed to lethal and sublethal concentrations in groups of 10 fish in 15 L of the test water in test chambers for 1 and 8 days. Fluctuations in succinate dehydrogenase (SDH), lactate dehydrogenase (LDH) and malate dehydrogenase (MDH) were observed in different tissues of the experimental animal. The activity of LDH was highly elevated following profenofos and carbosulfan exposure indicating increased anaerobic respiration to meet the energy demands where aerobic oxidation is lowered. Rapid depletion of SDH activity in all tissues of fish, L. rohita treated with sublethal and lethal concentrations of profenofos and carbosulfan. When compared to their respective controls. The general reduction in SDH activity due to pesticidal stress was associated with the inhibition of mitochondrial respiratory mechanism of dearrangement on ultra-structure, architectural integrity and permeability of mitochondria. Decreased MDH activity levels due to the inhibition exerted by oxaloacetate; maybe decrease in the activity of TCA cycle dehydrogenase is consistent with the disintegration of mitochondria of CO2 formation from acetate. Both profenofos and carbosulfan intoxication exerted a profound influence on the dehydrogenase enzymes of fish L. rohita but comparatively profenofos treated fish tissues showed more deterioration when compared to carbosulfan treated fish.
本试验旨在研究异丙酚和硫丹对淡水鱼脱氢酶活性的影响。健康淡水鱼Labeo rohita (Hamilton)采自印度Nandivelugu养鱼场。根据初步致死毒性测试(96小时LC50即100 μg -1),从印度Guntur当地的农药商店购买了丙诺福和硫丹农药;1.2 mg l-1和1/10 96小时LC50即10 μg l-1;致死和亚致死浓度分别为0.12mg l-1和)。驯化后,将鱼以10尾为一组,在15 L的试验水中分别暴露于致死和亚致死浓度的环境中1天和8天。观察实验动物不同组织中琥珀酸脱氢酶(SDH)、乳酸脱氢酶(LDH)和苹果酸脱氢酶(MDH)的波动。在暴露于异丙酚和硫丹后,LDH的活性高度升高,表明在有氧氧化降低的情况下,厌氧呼吸增加以满足能量需求。经亚致死浓度和致死浓度的异丙磷和硫丹处理后,鱼体内SDH活性迅速降低。与他们各自的控制相比。农药胁迫导致的SDH活性普遍降低与线粒体脱序对线粒体超结构、结构完整性和通透性的抑制有关。由于草酰乙酸的抑制,MDH活性水平降低;TCA循环脱氢酶活性的降低可能与乙酸形成CO2时线粒体的解体相一致。对罗氏L. rohita脱氢酶均有较深的影响,但相对于对罗氏L. rohita脱氢酶,对罗氏L. rohita脱氢酶的影响更大。
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引用次数: 5
The Relationship between Mental Workload and Prevalence of Musculoskeletal Disorders among Welders of Tehran Heavy Metal Structures Company in 2016 2016年德黑兰重金属结构公司焊工精神负荷与肌肉骨骼疾病患病率的关系
Pub Date : 2016-11-26 DOI: 10.15412/J.JBTW.01051203
S. Zare, N. Hasheminezhad, Tania Dehesh, Davoud Hasanvand, Saeid Ahmadi, Rasoul Hemmatjo
"Mental workload refers to the amount of effort put in by the mind during the accomplishment of a duty. It essentially has to do with individuals’ mental capacity and the way they receive and process information in order to make decisions and take appropriate actions. The present study aimed at investigating the relationship between mental workload and prevalence of musculoskeletal disorders among welders of Tehran Heavy Metal Structures Company. This cross-sectional descriptive study was conducted in 2016. The sample included 100 welders working at Tehran Heavy Metal Structures Company. Sampling was done by census method. A demographic features questionnaire, NASA-TLX workload questionnaire, and Nordic Questionnaire were used for data collection. Data were analyzed by the use of SPSS (Version 22). Various data analysis procedures, including independent samples t-test, Pearson correlation, chi-square, and Fisher’s exact test, were employed. The mean of participants’ overall score in mental workload was 65.88 ± 21.15. Eighty four percent of the studied welders reported that they had experienced musculoskeletal disorders during the past 12 months (leading to the study), with the highest prevalence being recorded for waist (65%). Furthermore, the results of Pearson correlation indicated that there was a significant relationship between the prevalence of disorders in the legs and physical demand (P = 0.032). Also, a considerable association was observed between disorders in the wrist / hand and overall NASA mental workload score during the past 12 months (P = 0.021). Mental workload and the prevalence of musculoskeletal disorders are relatively high among welders. Waist was the most important risk factor among the participants studied. Future studies should attempt to detect risk factors that cause such disorders and suggest ways to reduce or prevent these problems among welders as much as possible."
“精神工作量指的是在完成一项任务时,大脑所付出的努力。它本质上与个人的心理能力以及他们接收和处理信息的方式有关,以便做出决定并采取适当的行动。本研究旨在探讨德黑兰重金属结构公司焊工精神负荷与肌肉骨骼疾病患病率之间的关系。本横断面描述性研究于2016年进行。样本包括100名在德黑兰重金属结构公司工作的焊工。抽样采用人口普查方法。采用人口统计学特征问卷、NASA-TLX工作量问卷和北欧问卷进行数据收集。数据分析采用SPSS (Version 22)软件。采用各种数据分析程序,包括独立样本t检验、Pearson相关检验、卡方检验和Fisher精确检验。心理负荷总分平均值为65.88±21.15。84%的被研究焊工报告说,他们在过去的12个月里经历过肌肉骨骼疾病(导致这项研究),其中腰部的患病率最高(65%)。此外,Pearson相关结果显示,腿部疾病患病率与身体需求之间存在显著相关(P = 0.032)。此外,在过去的12个月中,腕/手的疾病与NASA的总体精神工作量评分之间存在相当大的关联(P = 0.021)。焊工的精神负荷和肌肉骨骼疾病的患病率相对较高。腰围是研究对象中最重要的风险因素。未来的研究应试图发现导致此类疾病的风险因素,并提出尽可能减少或预防焊工这些问题的方法。”
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引用次数: 7
Evaluation of the Quality of Services Delivered in Qazvin’s Hospitals to Attract Medical Tourists: Joint Commission International Approach 卡兹温医院吸引医疗游客的服务质量评价:国际联合委员会方法
Pub Date : 2016-11-12 DOI: 10.15412/J.JBTW.01051204
R. Kalhor, S. Moosavi, M. Ramezanpour, A. Molapour, A. Keshavarz, E. Bahrami, M. Azmal, Fatemeh Gharaghieh
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引用次数: 1
Ranking Eastern Mediterranean Region Countries (EMRO) based on the Health Impact Indicators Using Multi-criteria Decision Approach 采用多标准决策方法根据健康影响指标对东地中海地区国家进行排名
Pub Date : 2016-11-11 DOI: 10.15412/J.JBTW.01051202
R. Kalhor, S. Asefzadeh, F. Ghamari
"Health indicators and their determinants are important in appropriate policy making at the national level and throughout the world. This study attempts to explain the health impact indicators and rank the eastern Mediterranean region’s countries using the combination approach of TOPSIS-AHP. This study is both descriptive and analytical, and is conducted through cross-sectional methods. In order to weight life expectancy and mortality indicators as impact indicators by AHP, 25 experts completed a paired comparison questionnaire. Expert Choice 11 was used to weight indicators and TOPSIS software was used to rank the countries. Based on the Analytic Hierarchy Process and paired comparisons in health impact indicators, it was found that the highest weight was related to the infant mortality rate (IMR) with a weight of 0.284, and the lowest weight was related to the life expectancy indicator at age 60 with a weight of 0.030. After ranking the countries according to the health impact indicators and by using the TOPSIS method, it was found that Bahrain is the first and Somalia is the last among the studied countries. Existing facilities and potentials shall be guided first to the countries with unsuitable health indicators; these countries shall be focused on more than other countries within the region. The most important strategies that the countries can apply to improve the health indicators are raising awareness about health related issues, eliminating financial barriers that decrease access to health, focusing on inter-sectoral cooperation, and promoting the other sectors to participate."
“健康指标及其决定因素对于国家一级和全世界的适当决策非常重要。本研究试图利用TOPSIS-AHP结合的方法解释健康影响指标,并对东地中海地区国家进行排名。本研究是描述性和分析性的,并通过横断面方法进行。为了将预期寿命和死亡率指标作为层次分析法的影响指标,25位专家完成了配对比较问卷。专家选择11用于对指标进行加权,TOPSIS软件用于对国家进行排名。通过层次分析法和健康影响指标的成对比较发现,体重最高的与婴儿死亡率(IMR)相关,权重为0.284;体重最低的与60岁预期寿命相关,权重为0.030。根据健康影响指标并使用TOPSIS方法对各国进行排名后发现,巴林在所研究的国家中排名第一,索马里排名最后。现有设施和潜力应首先引导到卫生指标不适当的国家;这些国家应得到比本地区其他国家更多的关注。各国可用于改善卫生指标的最重要战略是提高对卫生相关问题的认识,消除减少获得卫生服务的财政障碍,注重部门间合作,并促进其他部门参与。”
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引用次数: 2
Assessment of Response to Dopamine Agonist (Pramipexole) in the treatment of Migraine Patients with Restless Legs Syndrome 多巴胺激动剂(普拉克索)治疗偏头痛伴不宁腿综合征的疗效评价
Pub Date : 2016-10-26 DOI: 10.15412/J.JBTW.01051101
M. Mazdeh, M. Khazaei, M. Taheri
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引用次数: 0
Chemical Properties and Sensory Evaluation of Probiotic Yoghurt Manufactured with Aqueous Extract of Aloe vera 芦荟水提物制备益生菌酸奶的化学性质及感官评价
Pub Date : 2016-10-25 DOI: 10.15412/J.JBTW.01051102
R. Mahmoudi, Farzaneh Bajalanlou, P. Ghajarbeygi, B. Pakbin
In recent years, consumer demand for a new range of dairy products, including yoghurts, which have functional and designed sensory properties have increased. In the present research physicochemical, microbiological and sensory attributes of yogurts manufactured from cow milk with aqueous extract of Aloe vera and Lactobacillus casei before and after cold storage for different periods of time (1, 3, 5, 7 and 10 days) were investigated. Titrable acidity (TA) of examined yoghurts during storage period at 4°C increased and their pH decreased significantly (P<0.05). The percentages of Water Holding Capacity (WHC) and Syneresis of yoghurt samples through the 10 days storage period were significantly decreased and increased, respectively (P<0.05). Viability of L. casei was significantly higher in probiotic yoghurt samples than others with Aloe vera extract after the end storage time. Sensory evaluation of examined yoghurts showed that Aloe vera extract had no effect on sensory quality of probiotic yoghurt samples. It was concluded that probiotic yoghurt with 2.5% Aloe vera extract with low syneresis and high WHC had better physicochemical, microbiological and sensory properties in comparison with the other probiotic yoghurt samples.
近年来,消费者对包括酸奶在内的一系列新型乳制品的需求有所增加,这些产品具有功能性和设计的感官特性。研究了芦荟和干酪乳杆菌水提物在冷藏1、3、5、7、10天前后的理化、微生物学和感官特性。4℃贮藏期间,酸奶的可滴定酸度(TA)显著升高,pH显著降低(P<0.05)。10 d贮藏期内,酸奶样品的持水能力(WHC)百分比显著降低,Syneresis百分比显著升高(P<0.05)。最终储藏时间后,益生菌酸奶样品中的干酪乳杆菌活力显著高于添加芦荟提取物的酸奶样品。对酸奶的感官评价表明,芦荟提取物对益生菌酸奶样品的感官品质没有影响。综上所述,与其他益生菌酸奶样品相比,添加2.5%芦荟提取物、低协同作用、高WHC的益生菌酸奶具有更好的理化、微生物学和感官性能。
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引用次数: 5
Nonenzymatic Electrochemical Sensing of Hydrogen Peroxide Based on Gold Nanolayers Covered with Snow-like Nanoparticles 基于雪状纳米颗粒覆盖金纳米层的过氧化氢非酶电化学传感
Pub Date : 2016-10-15 DOI: 10.15412/J.JBTW.01051003
R. D. Vais, H. Heli
"Gold nanolayers covered with snow-like nanoparticles were firstly synthesized by the sonoelectrodeposition method at a high negative potential. The nanostructure was then applied to prepare a highly sensitive nonenzymatic sensor for hydrogen peroxide. The catalytic activity and sensitivity of the gold nanostructure toward the electroreduction of hydrogen peroxide was excellent without surface fouling and deterioration effects. The current related to the reduction of hydrogen peroxide rapidly and linearly depended on the concentration with a sensitivity of 0.24 A mol-1 dm3 cm-2 and a detection limit of 7.9 i­mol dm-3. The present hydrogen peroxide sensor was fabricated by a simple preparation method without using any specific enzyme or reagent, with an excellent catalytic activity, high sensitivity and selectivity, long-term stability, and antifouling property."
“覆盖雪状纳米颗粒的金纳米层首次通过高负电位的声电沉积方法合成。然后将该纳米结构应用于制备过氧化氢高灵敏度非酶传感器。金纳米结构对双氧水电还原的催化活性和灵敏度均较好,无表面结垢和变质效应。与过氧化氢还原有关的电流与浓度呈快速线性关系,灵敏度为0.24 a mol-1 dm3 cm-2,检出限为7.9 i μ -mol dm-3。本发明的过氧化氢传感器制备方法简单,不使用任何特定的酶或试剂,具有优异的催化活性、高灵敏度和选择性、长期稳定性和防污性能。
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引用次数: 0
Psychological problems and quality of life in patients with cardiac rhythm management device (CRMD) 使用心律管理装置(CRMD)患者的心理问题与生活质量
Pub Date : 2016-10-03 DOI: 10.15412/J.JBTW.01050203
H. Dehghani, M. Jorat, T. Salami, Seyed Khalil Forozannia, Marzieh Shamibaf, K. Dehghani
"The studies which have done on patients who have cardiac rhythm management device (CRMD such as ICD and pacemaker) showed that using this device has been accompanied by losing quality of life and mental health situation of patients. Anxiety in these patients has been related with their life quality. Purpose of this study was check of their psychological problems (anxiety and depression) and the relation of these factors with their quality of life. This study has been done by cross-sectional descriptive method. Sampling method is census of whole patients who have gone to AFSHAR heart center (YAZD) which the number of sample was about 110 (41.8 percent women and 58.2 percent men). The patients have been answered standard questionnaire of HADS (hospital anxiety depression scale) and quality of life (SF36). Analyzing data has been done by using spss 20 software. Anxiety, depression and quality of life mean in these patients are 5.24±4.06, 4.88±4.01 and 60.8±17.40.anxiety mean has a significant relation with mean quality of life of patients (P=0/000) and life quality of these patients have been decreased by anxiety and depression increasing amount. The patients who have reported high anxiety and depression mean are in low life quality group. With regard to the results of these studies paying attention to mental problems anxiety in patients who have used pacemaker and defibrillator devices is necessary, because it increases their quality of life and success probability of cure."
“对使用心律管理装置(如ICD和起搏器)的患者进行的研究表明,使用这种装置会导致患者的生活质量和精神健康状况下降。这些患者的焦虑与他们的生活质量有关。本研究的目的是检查他们的心理问题(焦虑和抑郁)以及这些因素与他们生活质量的关系。本研究采用横断面描述性方法。抽样方法是对到过AFSHAR心脏中心(YAZD)的全部患者进行普查,样本数量约为110例(女性41.8%,男性58.2%)。对患者进行医院焦虑抑郁量表(HADS)和生活质量量表(SF36)的标准问卷调查。采用spss 20软件对数据进行分析。焦虑、抑郁和生活质量的平均值分别为5.24±4.06、4.88±4.01和60.8±17.40。焦虑均值与患者平均生活质量有显著相关(P=0/000),患者的生活质量随焦虑和抑郁程度的增加而下降。高焦虑、高抑郁的患者均属于低生活质量组。根据这些研究的结果,关注使用过起搏器和除颤器的患者的精神问题是必要的,因为这可以提高他们的生活质量和成功治愈的可能性。”
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引用次数: 0
In vitro mutation induction on TCL explants of Lilium (Lilium spp.) with Ethyl Methane Sulfunate (EMS) 甲烷磺酸乙酯(EMS)对百合(Lilium spp.) TCL外植体的体外诱变诱导
Pub Date : 2016-09-27 DOI: 10.15412/J.JBTW.01051002
F. K. Akhar, Azade Khadem, A. Sharifi, Z. Nemati, M. Yazdi, A. Bagheri
"In vitro mutagenesis is considered as an efficient approach for increasing the plant diversity. Lilium is one of the important bulbous flowers that distributed widely over the world. The present study was carried out to investigate the effect of Ethyl Methane Sulfunate (EMS) on in vitro mutagenesis of Lilium spp. (cv. OT Geel and Robina). After surface sterilization bulb scales, they were treated with EMS solution. Then, they were cut into 3mm thickness TCL explants and incubated on regeneration medium. Results indicated that 0.2% EMS was the most effective concentration in enhancement of shoots numbers in both cultivars. Molecular changes in OT Geel and Robina mutants showed that mutation can induce high polymorphism, so that in OT Geel, 11 treatments were classified into 4 groups with similarity level 0.87% and in Robina, 11 treatments were grouped into 2 groups with similarity level 0.86%. In all treatments genetic variations were observed, but higher concentrations of EMS were more effective for mutation induction. The results indicate that the efficiency of ISSR marker for detection of genetic variants is high and leads to early selection of them. This study is the first report to identify EMS treated mutants using ISSR marker in Lilium plants."
体外诱变被认为是增加植物多样性的有效途径。百合是广泛分布在世界各地的重要球茎花卉之一。本文研究了甲烷磺酸乙酯(EMS)对百合(Lilium spp.)体外诱变的影响。OT Geel和Robina)。球茎鳞片表面消毒后,用EMS溶液处理。然后切成3mm厚的TCL外植体,在再生培养基上培养。结果表明,0.2% EMS对两个品种的芽数增加效果最好。OT Geel和Robina突变体的分子变化表明,突变可诱导高多态性,因此OT Geel中将11个处理分为4组,相似度为0.87%,Robina将11个处理分为2组,相似度为0.86%。在所有处理中都观察到遗传变异,但较高浓度的EMS对突变诱导更有效。结果表明,ISSR标记对遗传变异的检测效率高,有利于遗传变异的早期选择。本研究是首次利用ISSR标记在百合植物中鉴定EMS处理突变体。”
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引用次数: 1
Aflatoxin M1 contamination of cow’s raw milk in different seasons from Qazvin province, Iran 伊朗加兹温省不同季节生牛奶黄曲霉毒素M1污染
Pub Date : 2016-09-23 DOI: 10.15412/J.JBTW.01051001
P. Ghajarbeygi, M. Palizban, R. Mahmoudi, G. Khaniki, B. Pakbin
Aflatoxins are extremely teratogenic, mutagenic, toxic, and carcinogenic compounds. In the present study, 60 cow’s raw milk samples were collected from Qazvin province, Iran during Dec 2015 till July 2016. Enzyme-linked immunoabsorbent assay (ELISA) was applied to determine Aflatoxin M1 (AFM1) in the milk samples. AFM1 was detected in 34 raw milk samples ranging from 6.25×10-3 to 127.87×10-3 (part per billion). AFM1 contents in all positive samples were far below the US legal limit (0.5 ppb), but AFM1 in30% of the raw milk samples exceeded the EU legal limit (0.05) and 5% of the samples exceeded the Iran legal limit (0.1 ppb). This study indicates a high occurrence of AFM1 in cow’s raw milk especially in winter (40.71×10- 3ppb) but the level of contamination were not significantly different in various seasons (P<0.05). Since contamination of milk with AFM1 is a potential risk for human health, in order to prevent the repetition, milk and milk products should be controlled periodically. The levels of AFM1 contamination of milk in the present study showed that continuous examining of milk is necessary to improve public health and reduce consumer exposure to aflatoxins. Reducing the levels of AFB1 in animal feedstuffs can be regarded as initial step to control the transfer of AFM1 to the humans.
黄曲霉毒素是极具致畸性、诱变性、毒性和致癌性的化合物。在本研究中,从2015年12月至2016年7月在伊朗Qazvin省收集了60头奶牛的原料奶样本。采用酶联免疫吸收法(ELISA)检测牛奶样品中的黄曲霉毒素M1 (AFM1)。在34份原料奶样品中检测到AFM1,范围从6.25×10-3到127.87×10-3(十亿分之一)。所有阳性样品中AFM1含量均远低于美国法定限量(0.5 ppb),但30%的原料奶样品中AFM1超过欧盟法定限量(0.05),5%的样品超过伊朗法定限量(0.1 ppb)。本研究表明,AFM1在奶牛原料奶中的高发率尤其在冬季(40.71×10- 3ppb),但不同季节的污染水平差异不显著(P<0.05)。由于牛奶被AFM1污染是对人类健康的潜在风险,为了防止这种情况再次发生,应定期对牛奶和奶制品进行控制。本研究中牛奶中AFM1的污染水平表明,为了改善公众健康和减少消费者接触黄曲霉毒素,对牛奶进行持续检查是必要的。降低动物饲料中AFB1的含量可视为控制AFM1向人类转移的第一步。
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引用次数: 12
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