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Effect of Processing Methods on Proximate Composition of Cassava Varieties Manihot esculenta (Crantz) Before and After Infestation by Prostephanus truncatus (Horn) 加工方法对木薯品种马尼特(manhot esculenta (Crantz))侵染前后近似组成的影响
Pub Date : 2021-09-03 DOI: 10.9734/ejnfs/2021/v13i530420
Eze U. Miriam, N. J. Okonkwo, I. F. Chisom
In Africa, Prostephanus truncatus is a destructive pest of economic importance which has assumed a serious pest status on stored maize and dry cassava chips. This study investigates the effect of processing methods of cassava varieties Manihot esculenta on the proximate composition before and after exposure to P. truncatus. The four cassava varieties used for this study were; TMS 0505, TME 419, NR 8082 and TMS 0581 subjected to two processing method parboiling and plain sun-drying at temperature 28-34oC and relative humidity of 65-75%. The experiment was carried out in a Complete Randomized Design. Proximate composition was carried out to determine the Moisture, Ash, Crude fibre, Fat, Crude Protein and Carbohydrate of the different samples. Analysis of Variance was used for the statistical analysis of the data obtained. The result reveals that the proximate composition of the sundried chips are significant at P<0.05%. All the proximate contents of the sample were significant at P<0.05% before the introduction of P. trucatus. After the introduction of P. truncatus and the storage period of 30days, the sundried sample had a higher reduction in the moisture contents of TMS 0505 with 13.05±0.00a and carbohydrate contents of NR 8082 with 50.0±0.00a. The activity of P. truncatus also has a negative impact on some of the nutritional components of cassava chips.
在非洲,圆茎蚜是一种具有重要经济意义的破坏性害虫,对储存的玉米和干木薯片造成严重危害。研究了木薯品种马尼奥特(Manihot esculenta)不同加工方法对其暴露前后近似成分的影响。本研究使用的四个木薯品种是;TMS 0505, TME 419, NR 8082和TMS 0581在温度28-34℃,相对湿度65-75%的条件下,进行过沸煮和普通晒干两种处理方法。试验采用完全随机设计。采用近似组成法测定了不同样品的水分、灰分、粗纤维、脂肪、粗蛋白质和碳水化合物。采用方差分析对所得数据进行统计分析。结果表明,在P<0.05%的情况下,干屑的近似组成显著。所有样品的近似值含量均显著在P<0.05%。经干枯处理后的干枯样品中,TMS 0505的含水率降低了13.05±0.00a, NR 8082的碳水化合物含量降低了50.0±0.00a。该菌的活性对木薯片的某些营养成分也有负面影响。
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引用次数: 0
Determination of Physico-chemical Properties, Cholesterol and Vitamin A Levels of Vegetable Oils commonly Sold in Ado Ekiti Metropolis Ado Ekiti大都市里常见植物油理化性质、胆固醇和维生素A含量的测定
Pub Date : 2021-08-31 DOI: 10.9734/ejnfs/2021/v13i530419
F. Ishaya, A. A. Oladejo, A. Olaoye
Edible vegetable oils are very important resource that are in high demand globally, and used in a variety of ways as they are considered a concentrated source of energy for human beings and carriers of oil-soluble vitamins which supply the essential fatty acids that are required for a wide range of biological and physiological functions. This work was aimed at evaluating the physicochemical properties, cholesterol content and analyzes the vitamin A contents of commonly sold vegetable oils in Ado Ekiti metropolis. The physicochemical parameters such as density, acid value, iodine value, peroxide value and saponin value, cholesterol content as well as Vitamin A content were all analyzed using standard analytical methods. Results of the physicochemical analysis showed that there was no significant difference (p>0.05) in the densities of the oil samples, It was observed that sample 4 oil showed maximum (1.58 mgKOH/g) and sample 6 showed minimum (1.38 mg KOH/g) acid values. Highest iodine values were observed in sample 8 (68.13 g/I2/100g) and lowest in sample 4 (56.38 g/I2/100g). Peroxide value was found to range from 2.18 meq O2/kg to 2.67 meq O2/kg, while saponin value was highest in sample 6 (130.3 mg KOH/g) and lowest in sample 8 (122.4 mg KOH/g). All the oil samples were found to contain cholesterol which ranged from 1.21±0.04 mg/dl (sample 1) to 4.58±0.01 mg/dl (sample 4), while the vitamin A content ranged from 674.80±10.26 IU/g (sample 2) to 877.97±20.52 IU/g (sample 8). Findings from this research showed that the researched oils meet the acceptable physicochemical standard. However, the cholesterol levels were against the inscriptions of cholesterol free on the labels.
食用植物油是一种非常重要的资源,在全球都有很高的需求,并以各种方式使用,因为它们被认为是人类能量的集中来源和油溶性维生素的载体,提供了广泛的生物和生理功能所需的必需脂肪酸。本研究旨在评价Ado Ekiti大都市区常见植物油的理化性质、胆固醇含量及维生素A含量。采用标准的分析方法,对其理化参数如密度、酸值、碘值、过氧化值、皂苷值、胆固醇含量、维生素A含量等进行了分析。理化分析结果表明,样品4的酸值最高(1.58 mgKOH/g),样品6的酸值最低(1.38 mgKOH/g),样品4的酸值最高(1.58 mgKOH/g)。样品8的碘含量最高(68.13 g/I2/100g),样品4的碘含量最低(56.38 g/I2/100g)。过氧化值在2.18 ~ 2.67 meq O2/kg之间,样品6中皂苷值最高(130.3 mg KOH/g),样品8中最低(122.4 mg KOH/g)。样品1的胆固醇含量为1.21±0.04 mg/dl ~ 4.58±0.01 mg/dl,样品4的维生素A含量为674.80±10.26 IU/g ~ 877.97±20.52 IU/g,符合理化标准。然而,胆固醇水平与标签上的“无胆固醇”字样不符。
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引用次数: 0
Prevalence, Risk Factors and Management of Type 2 Diabetes, and its Predictors among Patients Using Multinomial Logistic Modeling Approach: Case of a Semi-Urban Cameroonian 2型糖尿病的患病率、危险因素和管理及其预测因子在多项Logistic建模方法中的应用:一个半城市喀麦隆人的案例
Pub Date : 2021-08-14 DOI: 10.9734/ejnfs/2021/v13i530416
K. G. Kaptso, William Tchabo, Winifred Manyaka, Isabelle Mulango, Mbafor Brain Chebelem, Amungwa Fonteh Athanasius, C. Mbofung
Background: Diabetes is a health problem worldwide. The prevalence of diabetes has been steadily increasing for the past three decades. Diabetes prevalence is growing most rapidly particularly in low- and middle-income countries. Areas undergoing rapid westernization and rapid nutrition transition are seeing the greatest increase in prevalence suggesting that environmental factors are important. Diabetes is known to have no cure but can be managed through diet and modification of lifestyles. The aim of this study was to evaluate the prevalence and the predictors of diabetes and its management in Kumba District Hospital (KDH). Study Design: A retrospective, and a cross sectional survey was done where semi structured questionnaires were administered to subjects. Methodology: Information from the hospital record for the past four years was used to evaluate the trend in the prevalence of diabetes. Information from questionnaires was used to assess the prevalence and management of the disease. Fasting plasma glucose was measured to know their diabetic status. Results: The results revealed an increasing trend in the prevalence but the increase was not statistically significant. The results showed that, there was an association between diabetes prevalence and management. The overall prevalence of diabetes from the study was evaluated at 43.98%. Age, level of education, dietary habit and alcohol were found to influence the prevalence of diabetes significantly. Management with respect to therapeutic education, and monitoring of blood sugar were statistically significant. The significant predictive variables of the occurrence of diabetes base on Ordinary least squares were found to be age, level of education, therapeutic education, alcohol consumption, sex, and frequency of eating white rice. Conclusion: The study revealed that, diabetes is highly prevalent among older persons and the less educated in KDH. Public health officials should educate the public on the risk factors of diabetes, and implement guidelines for adequate control and management.
背景:糖尿病是一个全球性的健康问题。在过去的三十年里,糖尿病的患病率一直在稳步上升。糖尿病患病率增长最为迅速,特别是在低收入和中等收入国家。正在经历快速西化和快速营养转变的地区,发病率增幅最大,这表明环境因素很重要。众所周知,糖尿病无法治愈,但可以通过饮食和改变生活方式来控制。本研究的目的是评估Kumba地区医院(KDH)糖尿病的患病率和预测因素及其管理。研究设计:采用回顾性和横断面调查,对受试者进行半结构化问卷调查。方法:使用过去四年的医院记录信息来评估糖尿病患病率的趋势。调查问卷的信息被用来评估疾病的流行和管理。测定空腹血糖以了解患者的糖尿病状态。结果:发病率呈上升趋势,但无统计学意义。结果表明,糖尿病患病率与管理之间存在关联。研究中糖尿病的总患病率评估为43.98%。年龄、受教育程度、饮食习惯和酒精对糖尿病的患病率有显著影响。治疗教育和血糖监测方面的管理具有统计学意义。基于普通最小二乘的糖尿病发生的显著预测变量为年龄、文化程度、治疗教育、饮酒、性别和吃白米饭的频率。结论:本研究显示,糖尿病在嘉德区老年人及受教育程度较低的人群中高发。公共卫生官员应该教育公众糖尿病的危险因素,并实施适当控制和管理的指导方针。
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引用次数: 0
A Preliminary Risk Assessment of Yersinia Enterocolitica in the Food Chain: Some Aspects Related to Human Health in Norway 食物链中小肠结肠炎耶尔森菌的初步风险评估:挪威与人类健康有关的一些方面
Pub Date : 2021-07-31 DOI: 10.9734/ejnfs/2021/v13i530412
T. Nesbakken, G. Kapperud, J. Lassen, B. Lunestad, E. Rimstad, L. Robertson, E. Skjerve, Y. Wasteson, H. Kruse
This preliminary risk assessment is a result of self-tasking by the Panel on Biological Hazards, Norwegian Scientific Committee for Food Safety. The suggestion was offered to the Norwegian Food Safety Authority (Mattilsynet), which responded and requested a risk profile, or a preliminary risk assessment, to evaluate whether a full risk assessment would be needed at a later date. Yersinia enterocolitica is one of a few zoonotic bacteria that have a stable reservoir within the domestic animal population in Norway. This bacterial species has been isolated from human patients with acute enteritis, who sometimes exhibit symptoms resembling appendicitis. Y. enterocolitica has attracted considerable attention due to its ability to cause serious post-infectious complications. Serious clinical consequences occur relatively often with Y. enterocolitica as a relatively high frequency of people in Norway possess the tissue type HLA-B27. A severe sequela linked to this tissue type is reactive arthritis. The cold climate in Norway may enhance growth of Y. enterocolitica. Although the predominant cause of yersiniosis in Norway is Y. enterocolitica O:3, and the pig is considered the main source of infection, the relative contribution of pork consumption compared with other risk factors, for example drinking untreated water, is unknown. In Norway, a decline in human cases of yersiniosis has been recorded since the beginning of the 1990s. This decline has been attributed to implementation of improved slaughtering methods, including enclosure of the anus into a plastic bag after rectum-loosening. In Norway, most fattening pigs are slaughtered at the age of 150 to 180 days. By this age the tonsils may be an even more significant source of human pathogenic Y. enterocolitica than intestinal contents, since the occurrence in the intestinal tract and faeces is reduced at the time of slaughter. Accordingly, hygienic handling of the head and the plucks during slaughter and dressing is very important to avoid contamination of the carcass. The most efficient way to limit the spread from tongue and tonsils is probably decapitation early on in the carcass dressing procedure. In such a procedure, the head, including tongue and tonsils, should be removed on a separate line. Also, avoidance of incision of the sub-maxillary lymph nodes might reduce the spread, Epidemiological data suggest that it is possible to reduce the herd prevalence of Y. enterocolitica O:3 by minimising contact between infected and noninfected herds. Further, attempts to reduce the prevalence at the top levels of the breeding pyramids may be beneficial for the industry as a whole. The meat industry might be able to categorise herds using serological methods, and use these results in its strategy to reduce the risks for consumers. However, such a strategy has to be evaluated in a cost benefit context. The apparently low prevalence of pathogenic Y. enterocolitica in food may be due to lack of suitable select
这一初步风险评估是挪威食品安全科学委员会生物危害小组自行完成任务的结果。该建议被提交给挪威食品安全局(Mattilsynet),该机构回应并要求提供风险概况或初步风险评估,以评估是否需要在晚些时候进行全面风险评估。小肠结肠炎耶尔森菌是挪威家畜种群中具有稳定宿主的几种人畜共患细菌之一。这种细菌已从人类急性肠炎患者中分离出来,这些患者有时表现出类似阑尾炎的症状。小肠结肠炎因其能够引起严重的感染后并发症而引起了相当大的关注。严重的临床后果发生相对频繁的小肠结肠炎,因为挪威人具有相对高频率的组织型HLA-B27。与这种组织类型相关的严重后遗症是反应性关节炎。挪威寒冷的气候可能促进小肠结肠炎菌的生长。虽然挪威耶尔森菌病的主要病因是小肠结肠炎耶尔森菌O:3,并且猪被认为是主要的感染源,但与其他危险因素(例如饮用未经处理的水)相比,猪肉消费的相对贡献尚不清楚。在挪威,自1990年代初以来,人类感染耶尔森菌病的病例有所减少。这种下降归因于实施改进的屠宰方法,包括在直肠松动后将肛门装入塑料袋。在挪威,大多数育肥猪在150至180天的时候被屠宰。到这个年龄,扁桃体可能是比肠道内容物更重要的人类致病性小肠结肠炎耶氏菌来源,因为在屠宰时肠道和粪便中的发生率减少了。因此,在屠宰和包扎期间,对头和拔毛的卫生处理对于避免对胴体的污染非常重要。限制从舌头和扁桃体传播的最有效方法可能是在胴体修整过程的早期将其斩首。在这种手术中,头部,包括舌头和扁桃体,应该在一条单独的线上切除。此外,避免切开上颌下淋巴结可能会减少传播。流行病学数据表明,通过尽量减少感染和未感染畜群之间的接触,有可能降低O:3型小肠结肠炎耶氏菌的畜群患病率。此外,试图降低养殖金字塔顶层的流行率可能对整个行业有益。肉类行业也许能够使用血清学方法对畜群进行分类,并将这些结果用于降低消费者风险的策略中。然而,这种策略必须在成本效益的背景下进行评估。致病性小肠结肠炎耶氏菌在食品中的流行率明显较低,可能是由于缺乏合适的筛选方法。微生物实验室常规使用的培养方法灵敏度不够。需要一种标准化的、灵敏度更高的基于dna的技术来检测临床、食品和环境样本中的小肠结肠炎耶氏菌。
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引用次数: 0
Assessment and Updating of the Fortification Model from 2006 2006年以来强化模式的评估与更新
Pub Date : 2021-07-30 DOI: 10.9734/ejnfs/2021/v13i430405
M. Haugen, J. Dierkes, W. Frølich, L. Frøyland, R. Halvorsen, P. Iversen, J. Lyche, M. A. Mansoor, H. Meltzer, B. Skålhegg
In 2006 the, the Panel on Nutrition, Dietetic Products, Novel Food and Allergy in the Norwegian Scientific Committee for Food Safety (VKM) adapted a Danish model for assessing applications concerning food fortification into Norwegian conditions. The fortification model is presently used by the Norwegian Food Safety Authorities as a tool in the management of applications on food fortification.   The model from 2006 was based on intake calculations from dietary surveys from 1997-2000. Since then, new national dietary surveys have been published. These are the comprehensive nationwide Norwegian dietary surveys among adults (Norkost 3, 2010-2011), among young children (Småbarnskost, 2007) and infants (Spedkost, 2006-2007). The Norwegian Food Safety Authority has requested VKM to implement the new data into the fortification model from 2006.   In the model from 2006 it is assumed that 25% of the energy in the diet can be derived from fortified foods and drinks. Information from the Norwegian Food Safety Authority, including about a pilot study for Norkost 3 suggested that the overall intake of fortified foods and drinks was marginal. From management of applications for fortified foods, the Norwegian Food Safety Authority also experienced that there are few fortified foods on the market in Norway.  The Norwegian Food Safety Authority has therefore requested VKM to evaluate whether the assumption that 25 energy percent (E%) deriving from fortified foods can be reduced to 15 E%, and if such a reduction will have health implications. In addition, the Norwegian Food Safety Authority has asked VKM to perform an evaluation of the safety factors in the model.   VKM argues that the model for fortification should be based on the dietary intake of vitamins and minerals at the 95th percentile level in various age groups. This is in accordance with risk assessments performed in European Food Safety Authority (EFSA), and will assure that the dietary intake in a majority of the population will be covered, still within a reasonable secure use of dietary exposure calculations. Mean intake of vitamins and minerals from food supplements (among users only) was chosen, in an attempt to reduce the impact of those with a high intake of supplements. The intake at 95th percentile from the diet plus the mean nutrient intake from supplements is deducted from the tolerable upper intake level (UL) for each nutrient in each age group, giving the maximum amount of nutrients that can be “allocated” for food fortification. The maximum amount of a nutrient that can be “allocated” is then distributed over the energy intake at the 95th percentile level. In this manner an estimate is made showing which age group is most likely to have an excessive intake of a certain nutrient.    VKM does not have access to any other information about available fortified foods on the Norwegian market than the information given by the Norwegian Food Safety Authority. However, based on this informa
2006年,挪威食品安全科学委员会(VKM)的营养、饮食产品、新型食品和过敏小组采用了丹麦的模式,以评估挪威食品强化应用的情况。强化模型目前被挪威食品安全当局用作管理食品强化应用的工具。2006年的模型是基于1997-2000年饮食调查的摄入量计算得出的。从那时起,新的国家饮食调查已经公布。这些是挪威全国范围内对成年人(Norkost 3, 2010-2011年)、幼儿(smamatbarnskost, 2007年)和婴儿(Spedkost, 2006-2007年)进行的全面膳食调查。挪威食品安全局已要求VKM从2006年起将新的数据应用到强化模型中。在2006年的模型中,假设饮食中25%的能量可以来自强化食品和饮料。来自挪威食品安全局的信息,包括关于Norkost 3的一项试点研究表明,强化食品和饮料的总体摄入量是微不足道的。从强化食品申请的管理来看,挪威食品安全局也发现,挪威市场上很少有强化食品。因此,挪威食品安全局要求VKM评估是否可以将来自强化食品的25%能量百分比(E%)减少到15%,以及这种减少是否会对健康产生影响。此外,挪威食品安全局已要求VKM对该模型中的安全因素进行评估。VKM认为,强化模式应以不同年龄组维生素和矿物质的膳食摄入量为基础,达到95%的水平。这符合欧洲食品安全局(EFSA)进行的风险评估,并将确保大多数人口的膳食摄入量将被覆盖,仍然在合理安全的膳食暴露计算范围内。选择从食品补充剂中摄取的维生素和矿物质的平均摄入量(仅限使用者),试图减少那些高摄入量补充剂的人的影响。从饮食中摄取的第95百分位加上从补充剂中摄取的平均营养素从每个年龄组中每种营养素的可耐受最高摄入量(UL)中扣除,得出可“分配”用于食品强化的最大营养素量。一种营养素可以“分配”的最大量,然后在能量摄入的第95个百分位数水平上进行分配。通过这种方式,可以估计出哪个年龄组最有可能过量摄入某种营养素。除了挪威食品安全局提供的信息外,VKM无法获得挪威市场上现有强化食品的任何其他信息。然而,基于这些信息,VKM认为从强化食品中摄入的能量减少到15%似乎是合理的。在这个修订后的强化模型中,从2006年开始的总能量摄入的25%将来自强化食品的假设已经减少到15%。这一调整意味着,每100千卡可以增加维生素D、维生素E、硫胺素、核黄素、烟酸、叶酸、维生素B12、维生素C和钙的添加量,而不会有超过UL的风险。维生素A、β -胡萝卜素、镁、铁、锌和铜的含量没有变化。表1和附录1给出了更详细的总结。营养、饮食产品、新型食品和过敏问题小组认为,这种强化管理模式将减少未经批准的食品强化可能造成的健康风险。
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引用次数: 0
A Risk Assessment of Shiga Toxin-producing Escherichia coli (STEC) in the Norwegian Meat Chain with Emphasis on Dry-cured Sausages 挪威肉类连锁店中产志贺毒素大肠杆菌(STEC)的风险评估,重点是干腌香肠
Pub Date : 2021-07-30 DOI: 10.9734/ejnfs/2021/v13i430404
E. Rimstad, L. Håvarstein, G. Kapperud, J. Lassen, B. Lunestad, T. Nesbakken, L. Robertson, E. Skjerve, Y. Wasteson
coli is part of the normal gastrointestinal microbial flora of humans and animals. E. coli bacteria causing enteric/diarrhoeal disease are categorized into different groups based on their virulence properties and pathogenic features in humans. Enterohaemorrhagic E. coli (EHEC) are E. coli strains that  cause bloody diarrhoea  and haemolytic uraemic  syndrome  (HUS)  in humans, and have a defined zoonotic association. The major virulence factor of EHEC (and the actual  cause  of HUS)  is  the  ability  to  produce Shiga  toxins  (Stx),  thus  the  name Shiga Toxin Producing E. coli (STEC).  With enteropathogenic Escherichia  coli  (EPEC),  the diarrhoea in these  patients  is  due  to  attaching  and  effacing  (A/E)  lesions  in  the  enteric epithelium.    This risk assessment  was  conducted  after  a  human  outbreak  of  STEC  O103  in  2006, associated with contaminated dry-fermented sausages.   The Norwegian Scientific Committee for Food Safety (Vitenskapskomitéen for mattrygghet), Panel on  Biological  Hazards,  was  asked  by  the  Norwegian  Food  Safety  Authority (Mattilsynet) for  a  risk  assessment  regarding  shiga  toxin-producing  E.  coli  (STEC)  in  the Norwegian meat chain, with emphasis on dry-cured sausages. In response, an ad hoc Working Group  of  experts was  appointed with  the mandate  to  draft  a  risk  assessment  regarding  this issue.   The current report approaches the task by following and analysing the entire process, from the origin of the meats at farm level, to the final production and storage of dry-cured sausages. An overall  aim of  the  report has been  to  identify  and describe potential  intervention options  in various parts of this chain.     The main conclusions from the risk assessment are as follows:   It is  not  possible  to  give  any  reliable  quantitative  estimates  of  the  current  risk associated with consumption of dry-cured sausages.    There are  no  clear  indications  of  any  general  change  in  the  epidemiology  of  STEC  infections in humans in Norway over the last decade.   There is no documentation that there has been any change in the occurrence of various STEC in the domestic animal reservoir during the last decade.   The combination of proper slaughter hygiene and use of  thermal decontamination of sheep,  cattle  and  pig  carcasses  represents  an  efficient  way  to  reduce  STEC contamination. This approach would not only cause a reduction in the contamination level of STEC, but also provide a general beneficial effect on the level of other enteric pathogens, such as Salmonella and Yersinia enterocolitica.   Proper use  of  starter  cultures  in  fermentation,  combined  with  higher  fermentation temperatures,  will  reduce  the  probability  of  growth  of  STEC  in  contaminated  drycured sausages.   A combination  of  higher  fermentation  temperatures,  a  lower  pH  during  the  process, and heat-treatment of  the  f
大肠杆菌是人类和动物正常胃肠道微生物菌群的一部分。引起肠道/腹泻疾病的大肠杆菌根据其对人类的毒力特性和致病性特征被分为不同的组。肠出血性大肠杆菌(EHEC)是引起人类出血性腹泻和溶血性尿毒综合征(HUS)的大肠杆菌菌株,并具有明确的人畜共患关系。肠出血性大肠杆菌(也是溶血性尿毒综合征的实际原因)的主要毒力因子是产生志贺毒素(Stx)的能力,因此被称为产志贺毒素大肠杆菌(STEC)。患有肠致病性大肠杆菌(EPEC),这些患者的腹泻是由于肠上皮的附着和消退(A/E)病变。这一风险评估是在2006年与受污染的干发酵香肠有关的产肠毒素大肠杆菌O103人间暴发之后进行的。挪威食品安全局(Mattilsynet)要求挪威食品安全科学委员会(vitenskapskomitsamen for mattrygghet)生物危害小组对挪威肉类链中产生志贺毒素的大肠杆菌(STEC)进行风险评估,重点是干腌香肠。为此,任命了一个特设专家工作组,其任务是起草关于这一问题的风险评估报告。目前的报告通过跟踪和分析整个过程来完成这项任务,从肉类在农场一级的起源,到干腌香肠的最终生产和储存。该报告的总体目标是确定和描述在这一链的各个部分的潜在干预方案。风险评估的主要结论如下:不可能对目前与食用干腌香肠有关的风险给出任何可靠的定量估计。在过去十年中,没有明确的迹象表明挪威人类产志贺毒素大肠杆菌感染的流行病学有任何普遍变化。没有文献表明,在过去十年中,家畜储存库中各种产志贺毒素大肠杆菌的发生率有任何变化。适当的屠宰卫生和对羊、牛和猪尸体进行热消毒相结合是减少产肠毒素感染的有效方法。这种方法不仅会降低产肠毒素大肠杆菌的污染水平,而且还会对其他肠道病原体(如沙门氏菌和小肠结肠炎耶尔森菌)的水平产生普遍有益的影响。在发酵过程中适当使用发酵剂,再加上较高的发酵温度,将降低受污染的干腌香肠中产大肠杆菌生长的可能性。较高的发酵温度、过程中较低的pH值和最终产品的热处理相结合,应有效消除因食用干腌香肠而传播产大肠杆菌感染的潜在风险。减少5个对数是可能的。现有的技术选择可以显著减少潜在病原体通过肉类,特别是通过干腌香肠的传播。最重要的数据缺口是缺乏关于挪威人类产肠毒素大肠杆菌感染实际发生情况的信息。有必要改进实验室诊断程序和流行病学监测,同时在卫生保健系统中改进报告和追踪。建议对各种家畜进行适当设计的基线研究,以提供关于各种血清型的发生及其存在的毒力因子的数据。此外,这将为与人类分离株进行比较提供更好的基础。
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引用次数: 4
A Qualitative Assessment of the Risks of Transmission of Microorganisms to Humans Resulting from the Consumption of Raw Milk and Raw Cream in Norway 对挪威食用生奶和生奶油导致微生物向人类传播的风险进行定性评估
Pub Date : 2021-07-30 DOI: 10.9734/ejnfs/2021/v13i430403
Y. Wasteson, H. Blom, K. Fossum, E. Høiby, J. Narvhus, L. Håvarstein, G. Kapperud, J. Lassen, B. Lunestad, T. Nesbakken, E. Rimstad, L. Robertson, E. Skjerve, H. Kruse
Pasteurisation of all consumer milk became mandatory in Norway in 1953, and this has been an important component of the protective measures that have reduced the incidence of milk and food borne diseases. In 2004, a complete recast of the hygiene legislation addressing both food hygiene and veterinary aspects was adopted by the European Union, the so-called “Hygiene package”. According to this legislation, each member state may, on its own initiative, prohibit or restrict the marketing of some foods like raw milk or raw cream, intended for direct human consumption within its territory. In response to this, the Norwegian Food Safety Authority (Mattilsynet) commissioned the Panel on Biological Hazards of the Norwegian Scientific Committee for Food Safety (Vitenskapskomitéen for mattrygghet), to prepare a risk assessment regarding the consumption of raw milk and raw cream. In response, an ad hoc Working Group of experts was appointed with the mandate to draft a risk assessment which should include the following components: identification and characterization of microbiological hazardous agents present in Norwegian raw milk; characterization of the public health consequences of these agents; assessment of the probability of transmission of these agents to humans by distribution of raw milk and cream. Additionally, the risk assessment should identify potential hazards to human health from the importation of raw milk, identify hazards associated with equipment used for production and storage of raw milk, and assess the risks associated with the potential transfer of antimicrobial resistance genes.   Observations concerning infections related to consumption of raw milk and raw cream in Norway, other European countries and North America, show that a number of pathogenic microorganisms, including emerging pathogens, can occur in raw milk and raw cream. These pathogenic microorganisms and their toxins may represent a real threat to human health. The panel concluded that the risks associated with E. coli O157:H7 and other EHEC, C. jejuni and L. monocytogenes in raw milk and cream are high. Furthermore the importation of raw milk to Norway may result in the (re)introduction of microorganisms, which have been eradicated, or never previously have been present, in Norway. This can have serious consequences for both human and animal health.
1953年,挪威对所有消费者的牛奶强制进行巴氏消毒,这是减少牛奶和食源性疾病发病率的保护措施的重要组成部分。2004年,欧盟对食品卫生和兽医方面的卫生立法进行了全面修订,即所谓的“卫生一揽子计划”。根据该立法,每个成员国可以主动禁止或限制在其领土内直接供人类食用的某些食品,如生牛奶或生奶油的销售。为此,挪威食品安全局(Mattilsynet)委托挪威食品安全科学委员会生物危害问题小组(vitenskapskomitsamen for mattryghet)编写一份关于食用生牛奶和生奶油的风险评估。为此,任命了一个特设专家工作组,其任务是起草一份风险评估报告,其中应包括以下内容:鉴定和描述挪威原料奶中存在的微生物危险剂;描述这些物质对公共卫生的影响;通过分发生牛奶和奶油评估这些病原体传染给人类的可能性。此外,风险评估应确定原料奶进口对人类健康的潜在危害,确定与生产和储存原料奶所用设备有关的危害,并评估与抗菌素耐药性基因潜在转移有关的风险。在挪威、其他欧洲国家和北美,对与食用生奶和生奶油有关的感染的观察表明,许多致病微生物,包括新出现的病原体,可能发生在生奶和生奶油中。这些致病微生物及其毒素可能对人类健康构成真正的威胁。该小组得出结论,生牛奶和奶油中的大肠杆菌O157:H7和其他肠出血性大肠杆菌、空肠梭菌和单核增生乳杆菌的风险很高。此外,向挪威进口原料奶可能导致(重新)引入微生物,这些微生物在挪威已经被根除,或者以前从未存在过。这可能对人类和动物健康造成严重后果。
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引用次数: 0
Assessment of Benefits and Risks of Probiotics in Processed Cereal-based Baby Foods Lactobacillus Paracasei ssp. Paracasei F19 加工谷物婴儿食品中益生菌的益处和风险评估Paracasei F19
Pub Date : 2021-07-30 DOI: 10.9734/ejnfs/2021/v13i430408
R. Halvorsen, J. Lassen, T. Midtvedt, J. Narvhus, J. Rugtveit, S. Yazdankhah, K. Eckner, W. Frølich, L. Frøyland, P. Iversen, G. Kapperud, I. T. Lillegaard, B. Lunestad, J. Lyche, A. Mansoor, H. Meltzer, T. Nesbakken, K. Nygård, L. Robertson, M. Tranulis, M. Tryland, M. Haugen
The Norwegian Scientific Committee for Food Safety (VKM) has appointed an ad hoc-group of experts to answer a request from the Norwegian Food Safety Authority regarding benefit and risk assessment of Lactobacillus paracasei ssp. paracasei F19 (F19) in processed cerealbased baby foods intended for small children 1-3 years. This assessment is based on the literature provided by the notifier as well as that found by a MEDLINE search.    A notification regarding two products of processed cereal-based baby foods (hereafter called cereals), intended for small children and supplemented with the bacterium F19 initiated this work.   A daily supply of a monoculture of a particular bacterial strain in large quantities to an age group without a fully established intestinal flora, may have unknown adverse effects. There are however, to our knowledge, no studies investigating possible short or long term adverse health effects of F19 in processed cereal-based baby food given to children 13 months onwards.   The documentation and information provided by the notifier regarding the genetic stability of F19 in the two products during processing and storage, is considered insufficient and does not allow any conclusions to be drawn.    Moreover, the documentation obtained is not conclusive regarding the antibiotic resistance pattern of the bacterial strain used in the products in question, as the information on different antibiotics is partly inconsistent. The information about specific localization (chromosomal, plasmid) of the resistance genes is not sufficient.    Studies demonstrate that F19, as well as other bacterial strains considered probiotic, is able to “crosstalk” with enterocytes in mice and that the result of the “crosstalk” depends upon the microbiota present. Whether F19 has a similar “crosstalk-profile” in humans is unknown. However, as the strain is originally of human origin, it seems reasonable to assume that such “crosstalk” may occur. Thus, before giving F19 daily for months and years, it seems reasonable to ask for additional molecular and physiological studies to unravel the functional impact of possible changes in genetic expression in children.    Lactobacillus infections do occasionally occur, mainly as bacteremia, endocarditis and localized infections (e.g. abscesses, peritonitis, and meningitis) in patients with severe underlying diseases. Most of them are elderly, but children are not excluded. The species most often isolated are L. casei and L. rhamnosus, followed by L. paracasei.    The increasing use of immunosuppressive therapy and broad spectrum antibiotics which are ineffective against Lactobacillus, might increase the importance of these bacteria as possible pathogens. In order to be able to draw any conclusions regarding beneficial effects of F19, there is a need for randomized placebo-controlled studies in larger populations and in the relevant age group.    According to EFSA, Lactobacillus paracasei ssp. paracase
挪威食品安全科学委员会(VKM)任命了一个特设专家组,以回答挪威食品安全局关于副干酪乳杆菌ssp的益处和风险评估的要求。paracasei F19 (F19),用于1-3岁幼儿的加工谷物婴儿食品。此评估基于通知人提供的文献以及MEDLINE搜索找到的文献。一份关于两种以谷物为基础的加工婴儿食品(以下简称谷物)产品的通知启动了这项工作,这两种产品用于幼儿,并添加了F19细菌。每天向没有完全建立肠道菌群的年龄组大量提供单一培养的特定菌株,可能会产生未知的不良影响。然而,据我们所知,目前还没有研究调查13个月以上的婴儿食品中含有的F19对健康可能产生的短期或长期不利影响。呈报人提供的关于两种产品在加工和储存期间F19的遗传稳定性的文件和信息被认为是不充分的,不能得出任何结论。此外,由于不同抗生素的信息部分不一致,所获得的文件对有关产品中使用的细菌菌株的抗生素耐药性模式并不是结论性的。抗性基因的特异性定位(染色体、质粒)信息不充分。研究表明,F19以及其他被认为是益生菌的菌株能够与小鼠肠细胞“串扰”,而“串扰”的结果取决于存在的微生物群。目前尚不清楚F19在人类中是否也具有类似的“串音特征”。然而,由于该菌株最初是人类的,因此假设可能会发生这种“串扰”似乎是合理的。因此,在连续数月或数年每天服用F19之前,似乎有理由要求进行额外的分子和生理研究,以阐明儿童基因表达可能发生的变化对功能的影响。乳酸菌感染偶尔也会发生,主要表现为菌血症、心内膜炎和局部感染(如脓肿、腹膜炎和脑膜炎)。他们大多是老人,但也不排除儿童。最常分离的种是干酪乳杆菌和鼠李糖乳杆菌,其次是副干酪乳杆菌。越来越多地使用免疫抑制疗法和广谱抗生素对乳酸菌无效,可能增加这些细菌作为可能的病原体的重要性。为了能够得出关于F19有益作用的任何结论,需要在更大的人群和相关年龄组中进行随机安慰剂对照研究。根据欧洲食品安全局的说法,副干酪乳杆菌。paracasei F19具有充分的特征。然而,所提供的文件不足以声称对健康有积极影响,因此F19不能证明是益生菌。目前还没有发表的F19在儿童中的剂量反应研究,既没有关于F19在胃肠道中的生存,也没有可能的负面健康影响。因此,无法评估与单一栽培F19的频率和/或剂量有关的潜在负面健康影响,例如抗菌素耐药性的传播或对儿童遗传表达的不利影响。
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引用次数: 0
Assessment of Benefits and Risks of Probiotics in Processed Cerealbased Baby Foods Bifidobacterium Lactis Bb12 加工谷物婴儿食品中益生菌的益处和风险评估
Pub Date : 2021-07-30 DOI: 10.9734/ejnfs/2021/v13i430406
R. Halvorsen, J. Narvhus, J. Lassen, T. Midtvedt, J. Rugtveit, S. Yazdankhah, L. Andersen, W. Frølich, L. Frøyland, M. Haugen, G. Kapperud, B. Lunestad, E. Høiby, H. Meltzer, T. Nesbakken, K. Nygård, Ø. Olsvik, J. Paulsen, L. Robertson, M. Tranulis, M. Tryland, E. Rimstad
The Norwegian Scientific Committee for Food Safety (VKM) has appointed an ad hoc-group of experts to answer a request from the Norwegian Food Safety Authority regarding benefit and risk assessment of B. lactis Bb12 in baby foods focusing on the age groups 4-6 months, 612 months and 1-3 years. This assessment is based on the literature provided by the notifier as well as that found by a MEDLINE search.    An notification for use of processed cereal-based baby foods (from now on called cereals) intended for infants and small children supplemented with the microorganism Bifidobacterium lactis (B. lactis) Bb12 in Norway initiated this work.     Studies of potential hazards and positive health effects from cereals containing B. lactis Bb12 intended for infants and young children have not been reported in the available literature. However, reports on safety of and positive health effects from infant and follow on formula supplemented with B. lactis Bb12 are available and have been assessed by VKM. In most of these clinical studies B. lactis Bb12 was administered in combination with other probiotic strains.   Clinical studies report no serious adverse events of infant formula supplemented with B. lactis Bb12. The effect of long term daily consumption of such supplemented formula by the actual age groups is not known.   A few studies have demonstrated some effect of supplementing baby food with probiotics, including B. lactis Bb12, on diarrhoea and atopic eczema while other studies do not show such effects. Thus, the scientific evidence for a favourable effect of supplementing formula or solid food with B. lactis Bb12, is weak and in some cases lacking.    There are no studies demonstrating a positive effect of cereals supplemented with B. lactis Bb12 intended for infants and small children.   Several health claims related to probiotics have been assessed by EFSA, including claims on reduction of gastro-intestinal discomfort, normal functioning of the alimentary tract, building of the natural intestinal barrier, improvement of the general immunity, mental and cognitive developments of children and immune system of children during growth. In the opinions so far, EFSA has concluded that a cause and effect relationship has not been established between the consumption of the probiotic containing products and the claimed effect. None of the products assessed so far contained B. lactis Bb12 (1 November 2009).   Commercially produced cereals are frequent given to infants and small children in Norway from an early age and this is particularly important for the establishment of the intestinal bacterial flora and the development of the intestinal mucosal immune system. According to the notifier, one portion (25gram) of the cereal powder contains 1 x 109 B. lactis Bb12 in monoculture. Taking into consideration that the daily intake is often greater than one portion of cereals, even in infants below 6 months of age, this would represent a daily intake
挪威食品安全科学委员会(VKM)任命了一个特设专家组,以回应挪威食品安全局关于婴儿食品中乳酸菌Bb12的益处和风险评估的要求,重点关注4-6个月、612个月和1-3岁年龄组。此评估基于通知人提供的文献以及MEDLINE搜索找到的文献。在挪威,一份关于添加微生物乳酸双歧杆菌(B. lactis) Bb12的用于婴儿和幼儿的加工谷物婴儿食品(从现在起称为谷物)的使用通知启动了这项工作。关于含乳芽杆菌Bb12的谷物对婴幼儿的潜在危害和积极健康影响的研究在现有文献中尚未报道。然而,关于添加乳酸菌Bb12的婴儿和婴幼儿配方奶粉的安全性和积极健康影响的报告是可用的,并已由VKM进行了评估。在大多数这些临床研究中,乳杆菌Bb12与其他益生菌菌株联合使用。临床研究报告,添加乳芽孢杆菌Bb12的婴儿配方奶粉未发生严重不良事件。实际年龄组长期每日食用这种补充配方的影响尚不清楚。一些研究表明,在婴儿食品中添加益生菌,包括乳杆菌Bb12,对腹泻和特应性湿疹有一定效果,而其他研究则没有显示出这种效果。因此,在配方食品或固体食品中添加乳酸菌Bb12的有利效果的科学证据很弱,在某些情况下甚至缺乏。目前还没有研究证明在谷物中添加乳酸菌Bb12对婴幼儿有积极作用。欧洲食品安全局已经评估了几种与益生菌有关的健康声明,包括减少胃肠道不适,消化道正常功能,建立天然肠道屏障,改善一般免疫力,儿童智力和认知发展以及儿童生长过程中的免疫系统。到目前为止,欧洲食品安全局的结论是,在食用含有益生菌的产品和声称的效果之间没有建立因果关系。截至2009年11月1日,所有经评估的产品均未含有乳弧菌Bb12。在挪威,商业生产的谷物经常从很小的时候就给婴儿和幼儿食用,这对肠道菌群的建立和肠黏膜免疫系统的发育尤为重要。根据呈报人的资料,在单一栽培中,一份(25克)谷物粉含有1 × 109个乳酸菌Bb12。考虑到即使在6个月以下的婴儿中,每天的摄入量也往往大于一份谷物,这意味着4-6个月的婴儿每天摄入1-2 × 109 cfu乳清杆菌Bb12, 6个月以上的婴儿甚至更多。如果相当数量的乳杆菌Bb12在运输到小肠后存活下来,它将代表一个主要的单一栽培供应,通常一天几次。由于肠道微生物群和免疫系统的不成熟和脆弱,4 - 6个月和6 - 12个月这两个年龄最低的年龄组由于每天摄入益生菌而对健康产生不良影响的风险最高。
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引用次数: 0
Triglyceride Composition of Almond Seed Oil (Terminalia catappa) Grown in Nigeria using GC-MS and 1H-NMR Spectroscopy 用GC-MS和1H-NMR分析尼日利亚产杏仁籽油的甘油三酯组成
Pub Date : 2021-07-23 DOI: 10.9734/EJNFS/2021/V13I430402
Afolayan S. Sunday, Fashanu Titilope Abosede, Enenche E. Daniel, Adediji A. Yetunde, Salawu R. Adenike, Lawal Israel Oluwasanmi
Almond (Terminalia catappa) seeds are rich in oil; however, their study has received limited attention, with researches focused mainly on their health potentials. The present study assesses the composition of the fatty acid (FA) components present in the almond seed oils extracted using soxhlet apparatus and analysed by 1H-NMR (Proton Nuclear Magnetic Resonance) and Gas Chromatography Mass Spectrometry (GC-MS). Generally, there was significant agreement between the results from the 1H-NMR and GC-MS analyses, however, 1H NMR gave more reliable and reproducible results. The GC-MS and 1H NMR results revealed that the oils contained oleic acid (>18 %), linoleic (>28%) linolenic acid (≤0.03 %) and saturated fatty acids (>44 %).
杏仁(Terminalia catappa)种子富含油脂;然而,对它们的研究受到的关注有限,研究主要集中在它们的健康潜力上。本研究评估了用索氏装置提取的杏仁籽油中脂肪酸(FA)成分的组成,并通过1H-NMR(质子核磁共振)和气相色谱-质谱(GC-MS)进行了分析。一般来说,1H-NMR和GC-MS分析的结果有显著的一致性,然而,1H NMR给出了更可靠和可重复性的结果。GC-MS和1H NMR结果表明,油中含有油酸(> 18%)、亚油酸(>28%)、亚麻酸(≤0.03%)和饱和脂肪酸(> 44%)。
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引用次数: 0
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European Journal of Nutrition & Food Safety
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