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Effect of Different Processing Methods on the Quality of Ackee Fruit Arils 不同加工方法对槭树果皮品质的影响
Pub Date : 2020-04-10 DOI: 10.9734/ejnfs/2020/v12i230195
J. Ampofo-Asiama, Angela Abla Zebede, B. Abakah, B. Quaye
Ackee (Blighiasapida) fruit has a nutritional composition comparable to other commonly consumed fruits although its consumption is limited by the presence of anti-nutrients. This study investigated the effect of processing on some anti-nutrients (oxalate and phytate) as well as on ascorbate, carotenoids and phenolic compounds in ackee arils. Changes in physicochemical (pH, titratable acidity, brix and color) were also analyzed. Ackee fruit was cut into arils and cooked for 15 min either by boiling in water at 100°C or steamed or fried in oil at 180°C. The unprocessed ackee aril had a pH, titratable acidityand brix of 5.79, 0.52% and 2.70, respectively. Significant changes in these physicochemical properties were only observed in the boiled arils while the highest change in color was observed in the steam fruits. Significant reductions in ascorbate levels of 49.27, 32.86 and 56.29% were observed after boiling, steaming and frying, although these processing methods did not significantly affect carotenoid and phenol levels. The levels of the anti-nutrients reduced significantly with oxalate reducing by 22.37, 26.67 and 37.42% and phytate levels reducing by 62.50, 66.67 and 54.17%, after boiling, steaming and frying, respectively.
猕猴桃(Blighiasapida)果实的营养成分与其他常见水果相当,尽管其消费量受到抗营养素的存在的限制。本研究考察了加工对猕猴桃果实中一些抗营养物质(草酸和植酸)以及抗坏血酸、类胡萝卜素和酚类化合物的影响。理化性质(pH、可滴定酸度、白度和颜色)的变化也进行了分析。将猕猴桃切成小块,在100°C的水中煮15分钟,或在180°C的油中蒸或炸15分钟。未加工猕猴桃果皮的pH值为5.79,可滴定酸度为0.52%,糖度为2.70。这些物理化学性质的显著变化仅在煮熟的果实中观察到,而在蒸汽果实中观察到的颜色变化最大。煮沸、蒸煮和油炸后,抗坏血酸水平显著降低了49.27%、32.86%和56.29%,尽管这些处理方法对类胡萝卜素和酚水平没有显著影响。水煮、蒸煮和煎炸后,抗营养物质含量分别下降22.37%、26.67%和37.42%,植酸含量分别下降62.50%、66.67%和54.17%。
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引用次数: 1
Food/Feed and Environmental Risk Assessment of Insect Resistant Genetically Modified Maize MON810 for Cultivation, Seed Production, Import, Processing and Feed Uses under Directive 2001/18/EC (Notification C/F/95/12/02) 根据指令2001/18/EC种植、种子生产、进口、加工和饲料用途的抗虫转基因玉米MON810的食品/饲料和环境风险评估(通知C/F/95/12/02)
Pub Date : 2020-01-24 DOI: 10.9734/ejnfs/2019/v11i430171
Å. Andreassen, P. Brandtzæg, M. Aasmo Finne, Askild Lorentz Holck, O. Junttila, Heidi Sjursen Konestabo, R. Meadow, A. Mikalsen, Kåre M. Nielsen, M. Sanden, Ville Erling Sipinen, H. Opsahl-Sorteberg, R. Vikse
In preparation for a legal implementation of EU-regulation 1829/2003, the Norwegian Environment Agency (former Norwegian Directorate for Nature Management) has requested the Norwegian Food Safety Authority (NFSA) to give final opinions on all genetically modified organisms (GMOs) and products containing or consisting of GMOs that are authorized in the European Union under Directive 2001/18/EC or Regulation 1829/2003/EC within the Authority’s sectoral responsibility.  The Norwegian Food Safety Authority has therefore, by letter dated 13 February 2013 (ref. 2012/150202), requested the Norwegian Scientific Committee for Food Safety (VKM) to carry out scientific risk assessments of 39 GMOs and products containing or consisting of GMOs that are authorized in the European Union. The request covers scope(s) relevant to the Gene Technology Act. The request does not cover GMOs that VKM already has conducted its final risk assessments on. However, the Agency requests VKM to consider whether updates or other changes to earlier submitted assessments are necessary.  MON810 notification C/F/95/12-02 is approved under Directive 90/220/EEC for cultivation, seed production, import and processing into feeding stuffs and industrial purposes since 22 April 1998 (Commission Decision 98/294/EC). In December 1997, food and food ingredients derived from the progeny of maize line MON810 were notified under Article 5 of Regulation (EC) No 258/97 on novel foods and novel food ingredients. In addition, existing food and feed products containing, consisting of or produced from MON810 were notified according to Articles 8 and 20 of Regulation (EC) No 1829/2003 and were placed in the Community Register in 2005.   Three applications for renewal of the authorisation for continued marketing of (1) existing food and food ingredients produced from MON810; (2) feed consisting of and/or containing maize MON810, and MON810 for feed use (including cultivation); and (3) food and feed additives, and feed materials produced from maize MON810 within the framework of Regulation (EC) No 1829/2003 were submitted in 2007.   Maize MON810 has previously been assessed by the VKM GMO Panel commissioned by the Norwegian Environment Agency in connection with the national finalisation of the procedure of the notification C/F/95/12/02 (VKM 2007a,b). In addition, MON810 has been evaluated by the VKM GMO Panel as a component of several stacked GM maize events (VKM 2005a,b,c, VKM 2007c, VKM 2008, VKM 2009, VKM 2012a). Due to the publication of new scientific literature and updated guidance for food/feed and environmental risk assessment of genetically modified plants, the VKM GMO Panel has decided to deliver an updated risk assessment of MON810.  The updated risk assessment of the maize MON810 is based on information provided by the applicant in the notification C/F/95/12/02 and application EFSA/GMO/RX/MON810, and scientific comments from EFSA and other member states made available on the EFSA websit
为了准备在法律上实施欧盟第1829/2003号法规,挪威环境署(原挪威自然管理局)要求挪威食品安全局(NFSA)就欧盟根据2001/18/EC号指令或第1829/2003/EC号法规授权的所有转基因生物(GMOs)和含有或由转基因生物组成的产品给出最终意见。因此,挪威食品安全局于2013年2月13日致函(ref. 2012/150202),要求挪威食品安全科学委员会(VKM)对欧盟授权的39种转基因生物和含有或由转基因生物组成的产品进行科学风险评估。该请求涵盖了与《基因技术法案》相关的范围。该请求不包括VKM已经进行了最终风险评估的转基因生物。但是,原子能机构请VKM考虑是否有必要对先前提交的评估进行更新或其他修改。MON810通知C/F/95/12-02根据指令90/220/EEC批准,自1998年4月22日起用于种植、种子生产、饲料和工业用途的进口和加工(委员会决定98/294/EC)。1997年12月,根据关于新型食品和新型食品成分的法规(EC) No 258/97第5条,从玉米品系MON810后代衍生的食品和食品成分得到了通报。此外,现有的含有、由MON810组成或由MON810生产的食品和饲料产品已根据法规(EC) No 1829/2003第8条和第20条进行了通报,并于2005年在共同体登记册中登记。三份继续销售(1)由MON810生产的现有食品和食品配料的续期申请;(2)由和/或含有玉米MON810组成的饲料,以及用于饲料(包括种植)的MON810;(3) 2007年提交了法规(EC) No 1829/2003框架内由玉米MON810生产的食品和饲料添加剂以及饲料材料。玉米MON810先前已由挪威环境署委托的VKM转基因生物小组就通知C/F/95/12/02 (VKM 2007a,b)的国家最终确定程序进行了评估。此外,MON810已被VKM转基因专家小组评估为多个转基因玉米事件(VKM 2005a、b、c、VKM 2007c、VKM 2008、VKM 2009、VKM 2012a)的组成部分。由于发表了新的科学文献和更新的转基因植物食品/饲料和环境风险评估指南,VKM转基因生物小组决定提供MON810的最新风险评估。玉米MON810的最新风险评估是基于申请人在通知C/F/95/12/02和申请EFSA/GMO/RX/MON810中提供的信息,以及欧洲食品安全局和其他成员国在欧洲食品安全局网站GMO Extranet上提供的科学评论。风险评估还考虑了其他同行评审的相关科学文献。VKM转基因生物专家组参照MON810在欧洲经济区(EEA)的预期用途,根据《挪威食品法》、《挪威基因技术法案》和《基因技术法案》、关于故意向环境中释放转基因生物的指令2001/18/EC以及转基因食品和饲料法规(EC) No 1829/2003所述原则,对MON810进行了评估。VKM转基因生物小组还决定考虑欧洲食品安全局转基因植物及其衍生食品和饲料风险评估指南(EFSA 2006, 2011a)、转基因植物环境风险评估指南(EFSA 2010)和转基因植物风险评估比较物选择指南(EFSA 2011b)中所述的适当原则。玉米MON810的科学风险评估包括转化过程的分子特征、载体的构建、表达、遗传和转基因结构的稳定性、农艺和表型特征的比较评估、营养评估、毒理学和过敏原性、对植物适应性的意外影响、基因转移的潜力、转基因植物与靶生物和非靶生物的相互作用及其对生物地球化学过程的影响。需要强调的是,根据挪威基因技术法案和与基因技术法案相关的影响评估法规,VKM任务不包括对可持续发展、社会效用和伦理考虑的贡献评估。因此,这些考虑因素不属于VKM转基因生物小组提供的风险评估的一部分。
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引用次数: 0
Food/Feed and Environmental Risk Assessment of Insect-resistant and Herbicide-tolerant Genetically Modified Maize 1507 x 59122 for Food and Feed Uses, Import and Processing under Regulation (EC) No 1829/2003 (EFSA/GMO/NL/2005/15) 根据法规(EC) No 1829/2003 (EFSA/GMO/NL/2005/15)对用于食品和饲料、进口和加工的抗虫和耐除草剂转基因玉米1507 x 59122的食品/饲料和环境风险评估
Pub Date : 2020-01-24 DOI: 10.9734/ejnfs/2019/v11i430164
A. Andreassen, P. Brandtzaeg, M. Finne, A. Holck, A. Jevnaker, O. Junttila, Heidi Sjursen Konestabo, R. Meadow, A. Mikalsen, K. Nielsen, M. Sanden, V. Sipinen, H. Opsahl-Sorteberg, R. Vikse
In preparation for a legal implementation of EU-regulation 1829/2003, the Norwegian Environment Agency has requested the Norwegian Food Safety Authority to give final opinions on all genetically modified organisms (GMOs) and products containing or consisting of GMOs that are authorized in the European Union under Directive 2001/18/EC or Regulation 1829/2003/EC within the Authority’s sectorial responsibility. The Norwegian Food Safety Authority has therefore, by letter dated 13 February 2013 (ref. 2012/150202), requested the Norwegian Scientific Committee for Food Safety (VKM) to carry out scientific risk assessments of 39 GMOs and products containing or consisting of GMOs that are authorized in the European Union. The request covers scope(s) relevant to the Gene Technology Act. The request does not cover GMOs that VKM already has conducted its final risk assessments on. However, the Agency requests VKM to consider whether updates or other changes to earlier submitted assessments are necessary.  The insect-resistant and herbicide-tolerant genetically modified maize 1507 x 59122 from Dow AgroSciences and Pioneer Hi-Bred International, Inc. is approved under Regulation (EC) No 1829/2003 for food and feed uses, import and processing since 28 July 2010 (Commission Decision 2010/432/EC).   Genetically modified maize 1507 x 59122 has previously been risk assessed by the VKM Panel on Genetically Modified Organisms (GMO), commissioned by the Norwegian Food Safety Authority and the Norwegian Environment Agency related to the EFSAs public hearing of the applications EFSA/GMO/NL/2005/15 and EFSA/GMO/NL/2005/28 in 2007 (VKM 2007a, 2008a). In addition, 1507 x 59122 has been evaluated by the VKM GMO Panel as single events and as a component of several stacked GM maize events (VKM 2004, VKM 2005a,b, VKM 2007b,c, VKM 2008b,c, VKM 2009a,b, VKM 2012). The risk assessment of the maize 1507 x 59122 is based on information provided by the applicant in the applications EFSA/GMO/NL/2005/15 and EFSA/GMO/NL/2005/28, and scientific comments from EFSA and other member states made available on the EFSA website GMO Extranet. The risk assessment also considered other peer-reviewed scientific literature as relevant.    The VKM GMO Panel has evaluated 1507 x 59122 with reference to its intended uses in the European Economic Area (EEA), and according to the principles described in the Norwegian Food Act, the Norwegian Gene Technology Act and regulations relating to impact assessment pursuant to the Gene Technology Act, Directive 2001/18/EC on the deliberate release into the environment of genetically modified organisms, and Regulation (EC) No 1829/2003 on genetically modified food and feed. The Norwegian Scientific Committee for Food Safety has also decided to take account of the appropriate principles described in the EFSA guidelines for the risk assessment of GM plants and derived food and feed (EFSA 2011a), the environmental risk assessment of GM plants (EFSA 2010) and the
为了准备在法律上实施欧盟第1829/2003号法规,挪威环境署已要求挪威食品安全局就欧盟根据第2001/18/EC号指令或第1829/2003/EC号法规授权的所有转基因生物(GMOs)和含有或由转基因生物组成的产品给出最终意见。因此,挪威食品安全局于2013年2月13日致函(ref. 2012/150202),要求挪威食品安全科学委员会(VKM)对欧盟授权的39种转基因生物和含有或由转基因生物组成的产品进行科学风险评估。该请求涵盖了与《基因技术法案》相关的范围。该请求不包括VKM已经进行了最终风险评估的转基因生物。但是,原子能机构请VKM考虑是否有必要对先前提交的评估进行更新或其他修改。由陶氏农业科学公司和先锋杂交国际公司共同开发的抗虫和抗除草剂转基因玉米1507 x 59122,根据法规(EC) No 1829/2003,自2010年7月28日起被批准用于食品和饲料用途、进口和加工(委员会决定2010/432/EC)。转基因玉米1507 x 59122此前已由挪威食品安全局和挪威环境署委托的VKM转基因生物小组(GMO)进行了风险评估,该小组与2007年EFSA对申请EFSA/GMO/NL/2005/15和EFSA/GMO/NL/2005/28的公开听证会有关(VKM 2007a, 2008a)。此外,1507 x 59122已被VKM转基因生物小组评估为单一事件和几个堆叠转基因玉米事件的组成部分(VKM 2004, VKM 2005a,b, VKM 2007b,c, VKM 2008b,c, VKM 2009a,b, VKM 2012)。玉米1507 x 59122的风险评估是基于申请人在申请EFSA/GMO/NL/2005/15和EFSA/GMO/NL/2005/28中提供的信息,以及欧洲食品安全局和其他成员国在欧洲食品安全局网站GMO Extranet上提供的科学评论。风险评估还考虑了其他同行评审的相关科学文献。VKM转基因生物小组参照1507 x 59122在欧洲经济区(EEA)的预期用途,根据挪威食品法案、挪威基因技术法案、基因技术法案、关于转基因生物故意释放到环境中的指令2001/18/EC、转基因食品和饲料法规(EC) No 1829/2003所述原则,对其进行了评估。挪威食品安全科学委员会还决定考虑欧洲食品安全局转基因植物及其衍生食品和饲料风险评估指南(EFSA 2011a)、转基因植物环境风险评估指南(EFSA 2010)和转基因植物风险评估比较物选择指南(EFSA 2011b)中所述的适当原则。玉米1507 x 59122的科学风险评估包括插入DNA的分子特征和新蛋白质的表达,农艺和表型特征的比较评估,营养评估,毒理学和过敏原性,对植物适应性的意外影响,基因转移的潜力,转基因植物与目标和非目标生物之间的相互作用,对生物地球化学过程的影响。需要强调的是,根据挪威基因技术法案和与基因技术法案相关的影响评估法规,VKM任务不包括对可持续发展、社会效用和伦理考虑的贡献评估。因此,这些考虑因素不属于VKM转基因生物小组提供的风险评估的一部分。转基因玉米堆1507 x 59122是在含有1507和59122事件的玉米自交系之间通过常规育种产生的。该杂交品种对某些鳞翅目和鞘翅目害虫具有保护作用,并对草铵膦除草剂具有耐受性。分子特性:由于在玉米1507 x 59122的生产中使用了常规育种方法,因此没有涉及额外的基因改造。Southern分析和PCR分析表明,1507和59122事件中的重组插入物在1507 × 59122玉米堆中被保留。在亲本1507和59122中证实了插入物的遗传稳定性。表型分析表明,杂种抗虫、耐除草剂性状稳定。Cry1F、Cry34Ab1/Cry35Ab1和PAT蛋白在种子和饲料中的表达水平被认为与单一事件中的表达水平相当。
{"title":"Food/Feed and Environmental Risk Assessment of Insect-resistant and Herbicide-tolerant Genetically Modified Maize 1507 x 59122 for Food and Feed Uses, Import and Processing under Regulation (EC) No 1829/2003 (EFSA/GMO/NL/2005/15)","authors":"A. Andreassen, P. Brandtzaeg, M. Finne, A. Holck, A. Jevnaker, O. Junttila, Heidi Sjursen Konestabo, R. Meadow, A. Mikalsen, K. Nielsen, M. Sanden, V. Sipinen, H. Opsahl-Sorteberg, R. Vikse","doi":"10.9734/ejnfs/2019/v11i430164","DOIUrl":"https://doi.org/10.9734/ejnfs/2019/v11i430164","url":null,"abstract":"In preparation for a legal implementation of EU-regulation 1829/2003, the Norwegian Environment Agency has requested the Norwegian Food Safety Authority to give final opinions on all genetically modified organisms (GMOs) and products containing or consisting of GMOs that are authorized in the European Union under Directive 2001/18/EC or Regulation 1829/2003/EC within the Authority’s sectorial responsibility. The Norwegian Food Safety Authority has therefore, by letter dated 13 February 2013 (ref. 2012/150202), requested the Norwegian Scientific Committee for Food Safety (VKM) to carry out scientific risk assessments of 39 GMOs and products containing or consisting of GMOs that are authorized in the European Union. The request covers scope(s) relevant to the Gene Technology Act. The request does not cover GMOs that VKM already has conducted its final risk assessments on. However, the Agency requests VKM to consider whether updates or other changes to earlier submitted assessments are necessary. \u0000 The insect-resistant and herbicide-tolerant genetically modified maize 1507 x 59122 from Dow AgroSciences and Pioneer Hi-Bred International, Inc. is approved under Regulation (EC) No 1829/2003 for food and feed uses, import and processing since 28 July 2010 (Commission Decision 2010/432/EC).  \u0000 Genetically modified maize 1507 x 59122 has previously been risk assessed by the VKM Panel on Genetically Modified Organisms (GMO), commissioned by the Norwegian Food Safety Authority and the Norwegian Environment Agency related to the EFSAs public hearing of the applications EFSA/GMO/NL/2005/15 and EFSA/GMO/NL/2005/28 in 2007 (VKM 2007a, 2008a). In addition, 1507 x 59122 has been evaluated by the VKM GMO Panel as single events and as a component of several stacked GM maize events (VKM 2004, VKM 2005a,b, VKM 2007b,c, VKM 2008b,c, VKM 2009a,b, VKM 2012). The risk assessment of the maize 1507 x 59122 is based on information provided by the applicant in the applications EFSA/GMO/NL/2005/15 and EFSA/GMO/NL/2005/28, and scientific comments from EFSA and other member states made available on the EFSA website GMO Extranet. The risk assessment also considered other peer-reviewed scientific literature as relevant.   \u0000 The VKM GMO Panel has evaluated 1507 x 59122 with reference to its intended uses in the European Economic Area (EEA), and according to the principles described in the Norwegian Food Act, the Norwegian Gene Technology Act and regulations relating to impact assessment pursuant to the Gene Technology Act, Directive 2001/18/EC on the deliberate release into the environment of genetically modified organisms, and Regulation (EC) No 1829/2003 on genetically modified food and feed. The Norwegian Scientific Committee for Food Safety has also decided to take account of the appropriate principles described in the EFSA guidelines for the risk assessment of GM plants and derived food and feed (EFSA 2011a), the environmental risk assessment of GM plants (EFSA 2010) and the ","PeriodicalId":11994,"journal":{"name":"European Journal of Nutrition & Food Safety","volume":"16 4","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-01-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91426783","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Food/Feed and Environmental Risk Assessment of Insect-resistant and Herbicide-tolerant Genetically Modified Maize 59122 x 1507 x NK603 for Food and Feed Uses, Import and Processing under Regulation (EC) No 1829/2003 (EFSA/GMO/UK/2005/21) 根据法规(EC) No 1829/2003 (EFSA/GMO/UK/2005/21)对用于食品和饲料、进口和加工的抗虫和耐除草剂转基因玉米59122 x 1507 x NK603的食品/饲料和环境风险评估
Pub Date : 2020-01-24 DOI: 10.9734/ejnfs/2019/v11i430168
Å. Andreassen, P. Brandtzæg, M. Aasmo Finne, Askild Lorentz Holck, A. Jevnaker, O. Junttila, Heidi Sjursen Konestabo, R. Meadow, A. Mikalsen, Kåre M. Nielsen, M. Sanden, H. Opsahl-Sorteberg, R. Vikse
In preparation for a legal implementation of EU-regulation 1829/2003, the Norwegian Scientific Committee for Food Safety (VKM) has been requested by the Norwegian Environment Agency (former Norwegian Directorate for Nature Management) and the Norwegian Food Safety Authority (NFSA) to conduct final food/feed and environmental risk assessments for all genetically modified organisms (GMOs) and products containing or consisting of GMOs that are authorized in the European Union under Directive 2001/18/EC or Regulation 1829/2003/EC. The request covers scope(s) relevant to the Gene Technology Act. The request does not cover GMOs that VKM already has conducted its final risk assessments on. However, the Agency and NFSA requests VKM to consider whether updates or other changes to earlier submitted assessments are necessary.  The insect-resistant and herbicide-tolerant genetically modified maize 59122 x 1507 x NK603 from Pioneer Hi-Bred International, Inc. (Unique Identifier DAS-59122-7 x DAS-Ø15Ø7-1 x MONØØ6Ø3-6) is approved under Regulation (EC) No 1829/2003 for food and feed uses, import and processing since 28 July 2010 (Commission Decision 2010/428/EU).   Genetically modified maize 59122 x 1507 x NK603 has previously been risk assessed by the VKM Panel on Genetically Modified Organisms (GMO), commissioned by the Norwegian Food Safety Authority related to the EFSAs public hearing of the application EFSA/GMO/NL/2005/20 in 2007 (VKM 2007a). In addition, 59122 x 1507 x NK603 has been evaluated by the VKM GMO Panel as single events and as a component of several stacked GM maize events (VKM 2004, VKM 2005a,b, VKM 2007b,c, VKM 2008b,c, VKM 2009a,b, VKM 2012).   The food/feed and environmental risk assessment of the maize 59122 x 1507 x NK603 is based on information provided by the applicant in the application EFSA/GMO/UK/2005/21, and scientific comments from EFSA and other member states made available on the EFSA website GMO Extranet. The risk assessment also considered other peer-reviewed scientific literature as relevant.    The VKM GMO Panel has evaluated 59122 x 1507 x NK603 with reference to its intended uses in the European Economic Area (EEA), and according to the principles described in the Norwegian Food Act, the Norwegian Gene Technology Act and regulations relating to impact assessment pursuant to the Gene Technology Act, Directive 2001/18/EC on the deliberate release into the environment of genetically modified organisms, and Regulation (EC) No 1829/2003 on genetically modified food and feed. The Norwegian Scientific Committee for Food Safety has also decided to take account of the appropriate principles described in the EFSA guidelines for the risk assessment of GM plants and derived food and feed (EFSA 2011a), the environmental risk assessment of GM plants (EFSA 2010), selection of comparators for the risk assessment of GM plants (EFSA 2011b) and for the post-market environmental monitoring of GM plants (EFSA 2011c).  The scientific risk as
比较评估:申请人提交了从欧洲和北美的田间试验中收集的饲料和谷物材料的成分数据。欧洲田间试验数据的对比分析表明,玉米组合59122 x 1507 x NK603在组成、农艺和表型上与传统玉米组合相当,除了通过表达Cry1F、Cry34Ab1、Cry35Ab1、PAT和CP4 EPSPS蛋白而引入的抗虫性和除草剂耐受性之外。然而,在北美的田间试验中,玉米59122 x 1507 x NK603的组成、农艺和表型特征与零分离比较物进行了比较。由于阴性分离物来自转基因生物,VKM转基因生物小组认为它们不适合具有安全使用历史的传统对应物。从阴性分离的田间试验中获得的数据仅被视为补充信息。基于对现有数据的评估,VKM转基因专家小组认为,玉米59122、1507和NK603的传统杂交产生的杂交玉米59122 x 1507 x NK603不会产生相互作用,从而导致构成、农艺和表型变化,从而引起安全问题。食品和饲料安全评估:一项为期42天的家禽饲养研究表明,用59122 x 1507 x NK603玉米制备的日粮没有亚慢性不良反应。生物信息学分析没有揭示任何已知的orf在亲本玉米事件中的表达,并且没有新表达的蛋白质与任何已知的毒素或过敏原相似。没有任何一种蛋白质引起IgE介导的过敏反应的报道。然而,一些研究表明,在过敏反应中,冷冻蛋白作为佐剂具有潜在的作用。啮齿动物的急性和重复剂量毒性试验未显示新表达的蛋白质的毒性作用。然而,这些试验并没有提供任何关于玉米59122 x 1507 x NK603堆叠事件可能产生的不利影响的额外信息。根据目前的知识,VKM转基因专家组得出结论,59122 x 1507 x NK603玉米在营养上与其传统玉米相当,并且与传统玉米相比,新表达的蛋白质不太可能在由59122 x 1507 x NK603玉米制成的食品和饲料中引入毒性或致敏性。环境风险评估:申请EFSA/GMO/UK/2005/21的范围包括进口和加工用于食品和饲料用途的玉米堆59122 x 1507 x NK603。考虑到玉米59122 x 1507 x NK603的预期用途(不包括种植),环境风险评估涉及在运输和加工过程中有活性的谷物意外释放到环境中,以及间接暴露,主要是通过饲喂玉米59122 x 1507 x NK603谷物的动物的粪便和粪便。玉米59122 x 1507 x NK603的存活、繁殖或传播特性没有改变,并且没有迹象表明,在玉米59122 x 1507 x NK603的种子意外释放到环境中的情况下,野生玉米植物传播和建立的可能性增加。玉米是玉米属在欧洲的唯一代表,在栽培之外没有杂交的野生或杂草亲缘种。VKM转基因生物小组认为,在挪威,基因从偶尔的野生转基因玉米植物流向传统玉米品种的风险可以忽略不计。考虑到作为食品和饲料的预期用途,转基因生物小组认为与生物和非生物环境的相互作用不是一个问题。总体结论:与传统玉米相比,VKM转基因小组尚未发现玉米59122 x 1507 x NK603或其加工产品的毒性或营养特性的改变。根据目前的知识,与传统玉米品种相比,新表达的蛋白质也不太可能增加玉米59122 x 1507 x NK603衍生的食品和饲料的致敏性。VKM转基因生物小组同样得出结论,根据目前的知识,玉米59122 x 1507 x NK603在挪威的预期用途方面与传统玉米品种的环境风险相当。
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引用次数: 0
Food, Feed and Environmental Risk Assessment of Glufosinatetolerant Genetically Modified Oilseed Rape T45 for Food and Feed Uses, Import and Processing Under Regulation (EC) No 1829/2003 (Application EFSA/GMO/UK/2005/25) 根据法规(EC) No 1829/2003(申请EFSA/GMO/UK/2005/25)对用于食品和饲料、进口和加工的耐草铵膦转基因油菜T45的食品、饲料和环境风险评估
Pub Date : 2020-01-24 DOI: 10.9734/ejnfs/2019/v11i430167
Å. Andreassen, P. Brandtzæg, M. Aasmo Finne, Askild Lorentz Holck, A. Jevnaker, O. Junttila, Heidi Sjursen Konestabo, R. Meadow, A. Mikalsen, Kåre M. Nielsen, M. Sanden, Ville Erling Sipinen, H. Opsahl-Sorteberg, R. Vikse
In preparation for a legal implementation of EU-regulation 1829/2003, the Norwegian Environment Agency (former Norwegian Directorate for Nature Management) has requested the Norwegian Food Safety Authority (NFSA) to give final opinions on all genetically modified organisms (GMOs) and products containing or consisting of GMOs that are authorized in the European Union under Directive 2001/18/EC or Regulation 1829/2003/EC within the Authority’s sectoral responsibility.  The Norwegian Food Safety Authority has therefore, by letter dated 13 February 2013 (ref. 2012/150202), requested the Norwegian Scientific Committee for Food Safety (VKM) to carry out scientific risk assessments of 39 GMOs and products containing or consisting of GMOs that are authorized in the European Union. The request covers scope(s) relevant to the Gene Technology Act. The request does not cover GMOs that VKM already has conducted its final risk assessments on. However, the Agency requests VKM to consider whether updates or other changes to earlier submitted assessments are necessary.  The assignment includes a scientific risk assessment of oilseed rape T45 from Bayer Crop Science (Unique Identfier ACS-BNØØ8-2) for food and feed uses, import and processing.   Food additives produced from T45 oilseed rape were notified in the EU as existing food additives within the meaning of Article 8 (1) (b) of Regulation 1829/2003, authorized under Directive 89/10/EEC (Community Register 2005). Feed materials produced from T45 were also notified as existing feed products containing, consisting of or produced from T45 according to Articles 8 and 20 of Regulation (EC) No 1829/2003 in 2003.    A notification for placing on the market of T45 according to the Directive 2001/18/EC was submitted in March 2004 (C/GB/04/M5/4), covering import and processing of T45 into food and feed. The application was further transferred into Regulation (EC) No 1829/2003 in November 2005 (EFSA/GMO/UK/2005/25). An application for renewal of authorisation for continued marketing of food additives and feed materials produced from T45 oilseed rape was submitted under Regulation (EC) No 1829/2003 in 2007 (EFSA/GMO/RX/T45). The EFSA GMO Panel performed one single comprehensive risk assessment for all intended uses of genetically modified oilseed rape T45 and issued a comprehensive scientific opinion for both applications submitted under Regulation (EC) No 1829/2003. The scientific opinion was published in January 30 2008 (EFSA 2008), and food and feed products containing or produced from oilseed rape T45 was approved by Commission Decision 26 March 2009 (Commission Decision 2009/184/EC).   The oilseed rape T45 is however currently being phased out (EU-COM 2009). The commercialisation of T45 oilseed rape seeds in third countries was stopped after the 2005 planting season and stocks of all oilseed rape T45 lines have been recalled from distribution and destroyed. The applicant commits not to commercialize the event in t
为了准备在法律上实施欧盟第1829/2003号法规,挪威环境署(原挪威自然管理局)要求挪威食品安全局(NFSA)就欧盟根据2001/18/EC号指令或第1829/2003/EC号法规授权的所有转基因生物(GMOs)和含有或由转基因生物组成的产品给出最终意见。因此,挪威食品安全局于2013年2月13日致函(ref. 2012/150202),要求挪威食品安全科学委员会(VKM)对欧盟授权的39种转基因生物和含有或由转基因生物组成的产品进行科学风险评估。该请求涵盖了与《基因技术法案》相关的范围。该请求不包括VKM已经进行了最终风险评估的转基因生物。但是,原子能机构请VKM考虑是否有必要对先前提交的评估进行更新或其他修改。该任务包括对拜耳作物科学(唯一标识符ACS-BNØØ8-2)的T45油菜进行科学风险评估,用于食品和饲料用途、进口和加工。根据指令89/10/EEC(共同体登记2005)授权,由T45油菜籽生产的食品添加剂在欧盟作为法规1829/2003第8 (1)(b)条意义上的现有食品添加剂进行通报。根据2003年法规(EC) No 1829/2003第8条和第20条,由T45生产的饲料材料也被通知为含有、由T45组成或由T45生产的现有饲料产品。2004年3月,根据指令2001/18/EC (C/GB/04/M5/4)提交了T45投放市场的通知,该通知涵盖了T45在食品和饲料中的进口和加工。该申请于2005年11月进一步转移到法规(EC) No 1829/2003 (EFSA/GMO/UK/2005/25)。2007年,根据法规(EC) No 1829/2003 (EFSA/GMO/RX/T45)提交了一份关于继续销售由T45油菜生产的食品添加剂和饲料材料的续期申请。欧洲食品安全局转基因生物小组对转基因油菜T45的所有预期用途进行了一次全面的风险评估,并对根据法规(EC) No 1829/2003提交的两项申请发表了全面的科学意见。该科学意见于2008年1月30日公布(EFSA 2008),委员会决定2009年3月26日批准了含有或由油菜T45制成的食品和饲料产品(委员会决定2009/184/EC)。然而,油菜T45目前正在逐步淘汰(EU-COM 2009)。2005年种植季后,T45油菜种子在第三国的商业化被停止,所有T45油菜品种的库存已从分销中召回并销毁。申请人承诺将来不会将该事件商业化,因此进口将被限制在油菜商品的未知水平。因此,油菜T45在欧盟的发病率预计是有限的。VKM转基因生物小组(GMO)此前已经对油菜T45进行了风险评估,该小组受美国国家食品安全局委托,与2007年欧洲食品安全局的公开听证会有关(VKM 2007a)。对油菜籽T45的风险评估是基于申请EFSA/GMO/UK/2005/25和EFSA/GMO/RX/T45中通知人提供的信息,以及欧洲食品安全局和其他成员国在欧洲食品安全局网站GMO Extranet上提供的科学评论。风险评估还考虑了其他同行评审的相关科学文献。VKM转基因生物专家组参照T45在欧洲经济区(EEA)的预期用途,根据《挪威食品法》、《挪威基因技术法案》、《基因技术法案》、关于转基因生物故意释放到环境中的指令2001/18/EC、关于转基因食品和饲料的法规(EC) No 1829/2003所述原则,对T45进行了评估。挪威食品安全科学委员会还决定考虑欧洲食品安全局转基因植物及其衍生食品和饲料风险评估指南(EFSA 2006, 2011a)、转基因植物环境风险评估指南(EFSA 2010)和转基因植物风险评估比较物选择指南(EFSA 2011b)中所述的适当原则。油菜T45的科学风险评估包括插入DNA的分子特征和新蛋白的表达、农艺和表型特征的比较评估、比较成分分析、食品/饲料安全评估和环境评估。 需要强调的是,根据挪威基因技术法案和与基因技术法案相关的影响评估法规,VKM任务不包括对可持续发展、社会效用和伦理考虑的贡献评估。因此,这些考虑因素不属于VKM转基因生物小组提供的风险评估的一部分。以普通油菜品种“AC Excel”原生质体为材料,通过农杆菌介导转化,获得了耐草膦铵油菜转化事件T45。T45含有从病毒色链霉菌(Streptomyces viridochromogenes,菌株Tü 494)中分离的天然pat基因的合成版本。插入的基因编码磷酸钠丙氨酸乙酰转移酶(PAT),该酶对草甘膦铵具有耐受性。PAT酶通过将l-异构体乙酰化成n -乙酰- l-草膦酸铵(NAG)来解毒草铵,这不会抑制谷氨酰胺合成酶,因此对除草剂具有耐受性。耐草铵膦油菜转化事件T45已被常规培育成一系列春型油菜品种。分子表征:分子表征数据表明,油菜基因组DNA中只有一个基因盒拷贝。对整合位点进行了适当的分析,包括插入DNA和侧翼区域的序列测定以及生物信息学分析。连接区域的生物信息学分析表明,没有任何潜在的新的orf编码已知毒素或过敏原。通过Southern分析,在基因组水平上证明了转化事件T45的遗传稳定性。分离分析表明,事件T45为显性单位点遗传性状。自1993年以来在加拿大种植的T45品系及其衍生品种对除草剂的稳定耐受性证实了表型稳定性。油菜转化事件T45和蛋白质的物理、化学和功能特性先前已由VKM转基因生物小组进行了评估,并认为令人满意(VKM 2007a)。比较评估:为进行成分分析,从加拿大进行的三次田间试验中收获种子(1995年、2000年和2004年)。这些田间试验采用了典型的商业油菜栽培的农艺做法和田间条件,并提供了具有代表性的油菜种植地理区域的环境情况。通过分析T45油菜与其非转基因亲本品种AC Excel或与其他由AC Excel导出的比较品种的差异,对分析数据进行统计学评价。经合组织共识文件(OECD 2011)中列出的关于油菜的几种成分尚未在种子、油或粕中进行分析,如维生素K和抗营养物质辛酸。对比邻物、纤维、氨基酸、维生素E (α、β、γ和δ生育酚)、总生育酚、矿物质(磷、铁、钙、钠、铜、镁、锰、钾和锌)、脂肪酸、植酸和硫代葡萄糖苷(alken glucosin、MSGL glucosin和吲哚硫代葡萄糖苷)进行成分分析。ELISA法仅在T45油菜烤粕中检测到微量的PAT蛋白,在混合、脱胶、精制、脱色和脱臭油中检测不到PAT蛋白。成分分析显示部分分析成分存在统计学差异。然而,这并不被认为与生物学相关,因为它在文献的参考范围内。根据1995-1997年在加拿大有代表性的地点和环境进行的田间试验数据的比较分析结果,得出结论:油菜T45在农艺和表型上与传统的对应品种和商业可用的参考品种相当,除了成熟度和PAT蛋白所带来的除草剂耐受性。田间评价支持这样一个结论,即与常规油菜相比,T45事件没有增加植物杂草/害虫潜力的表型变化。此外,结果表明,与常规油菜相比,草铵膦除草剂在作物中施用不会改变事件T45的表型和农艺性状。食品和饲料安全评价:将PAT蛋白的总氨基酸序列与公共数据库中列出的已知毒素和过敏原的氨基酸序列进行比较。根据这些结果,没有证据表明它与已知的有毒或致敏蛋白质有任何相似之处。对肉鸡进行了动物饲养试验。 本研究未显示草甘膦铵处理和未处理的事件T45对采
{"title":"Food, Feed and Environmental Risk Assessment of Glufosinatetolerant Genetically Modified Oilseed Rape T45 for Food and Feed Uses, Import and Processing Under Regulation (EC) No 1829/2003 (Application EFSA/GMO/UK/2005/25)","authors":"Å. Andreassen, P. Brandtzæg, M. Aasmo Finne, Askild Lorentz Holck, A. Jevnaker, O. Junttila, Heidi Sjursen Konestabo, R. Meadow, A. Mikalsen, Kåre M. Nielsen, M. Sanden, Ville Erling Sipinen, H. Opsahl-Sorteberg, R. Vikse","doi":"10.9734/ejnfs/2019/v11i430167","DOIUrl":"https://doi.org/10.9734/ejnfs/2019/v11i430167","url":null,"abstract":"In preparation for a legal implementation of EU-regulation 1829/2003, the Norwegian Environment Agency (former Norwegian Directorate for Nature Management) has requested the Norwegian Food Safety Authority (NFSA) to give final opinions on all genetically modified organisms (GMOs) and products containing or consisting of GMOs that are authorized in the European Union under Directive 2001/18/EC or Regulation 1829/2003/EC within the Authority’s sectoral responsibility.  The Norwegian Food Safety Authority has therefore, by letter dated 13 February 2013 (ref. 2012/150202), requested the Norwegian Scientific Committee for Food Safety (VKM) to carry out scientific risk assessments of 39 GMOs and products containing or consisting of GMOs that are authorized in the European Union. The request covers scope(s) relevant to the Gene Technology Act. The request does not cover GMOs that VKM already has conducted its final risk assessments on. However, the Agency requests VKM to consider whether updates or other changes to earlier submitted assessments are necessary. \u0000 The assignment includes a scientific risk assessment of oilseed rape T45 from Bayer Crop Science (Unique Identfier ACS-BNØØ8-2) for food and feed uses, import and processing.  \u0000 Food additives produced from T45 oilseed rape were notified in the EU as existing food additives within the meaning of Article 8 (1) (b) of Regulation 1829/2003, authorized under Directive 89/10/EEC (Community Register 2005). Feed materials produced from T45 were also notified as existing feed products containing, consisting of or produced from T45 according to Articles 8 and 20 of Regulation (EC) No 1829/2003 in 2003.   \u0000 A notification for placing on the market of T45 according to the Directive 2001/18/EC was submitted in March 2004 (C/GB/04/M5/4), covering import and processing of T45 into food and feed. The application was further transferred into Regulation (EC) No 1829/2003 in November 2005 (EFSA/GMO/UK/2005/25). An application for renewal of authorisation for continued marketing of food additives and feed materials produced from T45 oilseed rape was submitted under Regulation (EC) No 1829/2003 in 2007 (EFSA/GMO/RX/T45). The EFSA GMO Panel performed one single comprehensive risk assessment for all intended uses of genetically modified oilseed rape T45 and issued a comprehensive scientific opinion for both applications submitted under Regulation (EC) No 1829/2003. The scientific opinion was published in January 30 2008 (EFSA 2008), and food and feed products containing or produced from oilseed rape T45 was approved by Commission Decision 26 March 2009 (Commission Decision 2009/184/EC).  \u0000 The oilseed rape T45 is however currently being phased out (EU-COM 2009). The commercialisation of T45 oilseed rape seeds in third countries was stopped after the 2005 planting season and stocks of all oilseed rape T45 lines have been recalled from distribution and destroyed. The applicant commits not to commercialize the event in t","PeriodicalId":11994,"journal":{"name":"European Journal of Nutrition & Food Safety","volume":"41 5 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-01-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90027016","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
Relationship between Dose and Duration of Administration of Potassium Bromate on Selected Electrolytes and Hepatorenal Parameters in Male Albino Wistar Rats 溴酸钾给药剂量和时间与雄性白化Wistar大鼠选定电解质和肝肾参数的关系
Pub Date : 2020-01-15 DOI: 10.9734/ejnfs/2019/v11i430163
S. Gazuwa, J. Dabak, Kiri H. Jaryum, Ige Oluwa
Aim: To monitor the effects of dosage and duration of administering KBrO3 on some electrolytes and hepatorenal parameters in male albino Wistar rats. Study Design: 24 rats, mean weight of 181.3 g were grouped into 4 with 6 rats of each. Experiment spanned over 12 days. In the control group, animals were fed standard diet. Animals in the test groups were fed diet containing 67, 100 and 167 mg/kg dose of KBrO3 according to body weight. 2 rats from each group were sacrificed on the 4th, 8th and 12th days. Place and Duration of Study: University of Jos; 1 month including writing the report. Methods: Spectrophotometric and titrimetric techniques were applied. InStat3 statistical software was used to analyse the data obtained. P≤.05 was considered significant. Results: on the 4th day at 67mg/kg dose, showed raised serum activities (IU) of ALT, 41.0±9.6, and AST, 130.2±31.53, (P=.05). At 100 mg/kg dose, serum activities of ALT, 52.12±1.12, AST, 180.0±0.41, and level (g/L) of Total Proteins, TP, 67.77±0.35, were elevated (P=.05).On the 8th day at 67mg/kg dose, there were no significant increases (P>.05). At 100 mg/kg dose, only AST activity, 98.0±43.86, increased (P=.05). Levels of urea (UR) and creatinine (CR) were lower than the control at both 60 and 100 mg/kg dose. At 167mg/kg dose, level of TP and activities of ALT, and AST increased (P=.05) relative control. On the 12thday, treatments at 67 mg/kg dose raised the activities of ALT and AST (P=.05).At 100 mg/kg dose, level of creatinine, 106±19.2 µmol/L, was significantly (P=.05) elevated. For urea, mmol/L, test groups results (4.26±1.39; 6.70±2.01; 21.07±2.21) were higher (P=.05) relative control group. Activities of AST and ALP were raised (P=.05). On the 12th day at 167 mg/kg dose, TP, ALT, AST and ALP significantly (P=.05) elevated implying toxicity of KBrO3 is both dose and duration of exposure-depended. On 4th and 8th day of treatment, mean level of Cl- was significantly (P=.05) raised whereas HCO3- was not significantly (P>.05) increased. 12th day of experimentation resulted in dose, and duration of exposure dependent increase concentration of Cl- (P=.05). Conclusion: This compound could potentially cause injury to, especially hepatocytes and nephrons. It can also perturb the redox status of the cell with its attendant metabolic consequences; hence, moderate use is imperative.
目的:观察KBrO3给药剂量和时间对雄性白化Wistar大鼠部分电解质和肝肾参数的影响。研究设计:24只大鼠,平均体重181.3 g,每组4只,每组6只。试验为期12天以上。对照组饲喂标准饲料。试验组按体重分别饲喂KBrO3添加剂量为67、100和167 mg/kg的饲粮。各组分别于第4、8、12天处死2只大鼠。学习地点和时间:乔斯大学;1个月,包括写报告。方法:采用分光光度法和滴定法。采用InStat3统计软件对所得数据进行分析。P≤。0.05被认为是显著的。结果:67mg/kg剂量组第4天ALT血清活性(IU)为41.0±9.6,AST血清活性(IU)为130.2±31.53 (P= 0.05)。在100 mg/kg剂量下,血清ALT活性为52.12±1.12,AST活性为180.0±0.41,总蛋白(TP)含量为67.77±0.35 (g/L) (P= 0.05)。67mg/kg剂量组第8天无显著升高(P> 0.05)。100 mg/kg剂量组仅AST活性升高(98.0±43.86)(P= 0.05)。尿素(UR)和肌酐(CR)水平在60和100 mg/kg剂量下均低于对照组。167mg/kg剂量组TP水平、ALT、AST活性相对对照组升高(P= 0.05)。第12天,67 mg/kg剂量组ALT和AST活性升高(P= 0.05)。在100mg /kg剂量下,肌酐水平(106±19.2µmol/L)显著升高(P= 0.05)。对于尿素,mmol/L,试验组结果为(4.26±1.39;6.70±2.01;21.07±2.21),高于对照组(P= 0.05)。AST、ALP活性升高(P= 0.05)。在167 mg/kg剂量的第12天,TP、ALT、AST和ALP显著升高(P= 0.05),表明KBrO3的毒性与剂量和暴露时间有关。治疗第4、8天,各组血清Cl-平均水平显著升高(P= 0.05), HCO3-平均水平无显著升高(P> 0.05)。实验第12天,氯离子浓度随剂量和暴露时间的增加而增加(P= 0.05)。结论:该化合物对肝细胞和肾单位有潜在的损伤作用。它还可以扰乱细胞的氧化还原状态,并伴随代谢后果;因此,适度使用是必要的。
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引用次数: 2
Types and Classification of Pesticides Used on Tomatoes Grown in Mwea Irrigation Scheme, Kirinyaga County, Kenya 肯尼亚Kirinyaga县Mwea灌溉计划中番茄农药的种类和分类
Pub Date : 2019-11-27 DOI: 10.9734/ejnfs/2019/v11i230145
V. Momanyi, M. Keraka, D. Abong’o, P. Warutere
This study evaluated 403 farmers from the open field and greenhouse farms in Mwea Irrigation Scheme on the types and classification of pesticides used to control pests and diseases on tomatoes, in July 2017 to June 2018. Five greenhouse tomato farmers were purposively selected while sample size of 196 open field farmers, calculated using Fisher’s formula. Cross-Sectional design using a structured questionnaire, face to face interviews and focus group discussions with 201 farmers in the eight wards, Gathingiri, Tebere, Kangai, Wamumu, Murinduko, Nyangati, Mutithi and Thiba. Accuracy of the data was ensured by pre-testing the questionnaire on tomato farmers from a neighbouring Maragua sub-county, errors were corrected, and omissions added to the questionnaire. Descriptive statistics was carried out for frequencies, percentages, means, standard errors, variance and data subjected to T-test at 95% Confidence Interval to determine significant differences between variables. Results from the interviews revealed that farmers applied 57 and 12 pesticides under different trade names on tomatoes in the open field and greenhouse farms respectively. Pyrethroids, carbamates, nicotinoids, organophosphates, and organochlorines were applied on tomatoes among others. The 20 and 12 pesticides mainly used in open field and greenhouse farms were WHO Class II (60%) and WHO Class III (42%), respectively. Farmers heavily relied on different types of pesticides to control a wide range of major pests and diseases as Tuta absoluta and blight respectively. Chlorantraniliprole and mancozeb are the main pesticides used in tomatoes. Most pesticides, WHO toxic class II including pyrethroids and carbamates should be used following manufacturers’ recommendations to prevent human health risks. Training and awareness by the Ministry of agriculture, Kirinyanga County government  are needed on use of less toxic pesticides equally effective in controlling pests and diseases, such as WHO  classes III and IV and bio-pesticides with minimal negative effects on human  health.
本研究于2017年7月至2018年6月期间,对Mwea灌溉计划中开放农田和温室农场的403名农民进行了番茄病虫害防治农药的种类和分类评估。有目的地选择5个温室番茄农户,196个露天番茄农户的样本量,采用Fisher公式计算。横断面设计采用结构化问卷、面对面访谈和焦点小组讨论的方式,对来自加辛格里、特贝雷、康艾、瓦木穆、穆杜科、尼扬加提、穆提提和蒂巴八个区201名农民进行了调查。通过对邻近的马拉瓜副县的番茄农民进行问卷预测试,确保了数据的准确性,错误得到了纠正,遗漏的部分被添加到问卷中。对频率、百分比、均值、标准误差、方差进行描述性统计,并在95%置信区间进行t检验,以确定变量之间的显著性差异。访谈结果显示,农民在露天农场和温室农场的番茄上分别使用了57种和12种不同商品名称的农药。拟除虫菊酯、氨基甲酸酯、类烟碱、有机磷和有机氯等被施用于西红柿上。露天农田和温室农场主要使用的20种和12种农药分别为WHO II类农药(60%)和WHO III类农药(42%)。农民严重依赖不同类型的农药来控制各种主要害虫和疾病,分别是白叶枯病和枯萎病。氯虫腈和代森锰锌是番茄的主要农药。大多数农药,包括拟除虫菊酯和氨基甲酸酯,应按照制造商的建议使用,以防止人类健康风险。需要Kirinyanga县政府农业部就使用同样有效控制病虫害的毒性较低的农药进行培训和提高认识,例如世卫组织的III类和IV类农药以及对人类健康负面影响最小的生物农药。
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引用次数: 8
Food Information Requirements of the Indian Consumers 印度消费者的食品信息需求
Pub Date : 2019-11-22 DOI: 10.9734/ejnfs/2019/v11i230144
S. Ghimire, V. Kamalvanshi, D. Bunkar, R. Kumar
Because of increase in the complexity of the food supply chain, there is information asymmetry in the food industry. But, in other hand, due to increase in concern over health, sustainability, local production, genetically modified crops and welfare issues, there is increase in concern of consumers regarding food information. Similarly, food information has direct implication to food safety. Although nutritional labelling is common in India, there exist knowledge gap about the actual informational need of the Indian consumers regarding the food they are consuming. This paper using the primary data collected through the structured web questionnaire tries to explore what information are required by the Indian consumers regarding the food and calculates their average willingness to pay for food with higher information. Survey was conducted in the month of March-April, 2019. A total of 514 valid responses were considered in the study. It was found that maximum number of consumers are willing to know more about chemical used at any stage, followed by production and marketing information and dietary health restrictions, respectively. It is found that Indian consumers at an average willing to pay 11.06% more for food with higher level of food information, than ordinary food. This research is valuable for future research on food information and various authorities to make policies regarding food information.
由于食品供应链的复杂性增加,食品行业存在信息不对称。但是,另一方面,由于对健康、可持续性、当地生产、转基因作物和福利问题的关注增加,消费者对食品信息的关注也增加了。同样,食品信息对食品安全也有直接的影响。虽然营养标签在印度很常见,但印度消费者对他们所消费的食品的实际信息需求存在知识差距。本文使用通过结构化网络问卷收集的原始数据,试图探索印度消费者对食品需要哪些信息,并计算他们对信息较高的食品的平均支付意愿。调查于2019年3月至4月进行。本研究共考虑了514份有效回复。调查发现,愿意更多了解在任何阶段使用的化学品的消费者人数最多,其次是生产和销售信息以及饮食健康限制。研究发现,印度消费者平均愿意为食品信息水平较高的食品支付比普通食品多11.06%的费用。本研究对今后食品信息的研究和有关部门制定食品信息政策具有一定的参考价值。
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引用次数: 0
Environmental Risk Assessment of Glufosinate-Tolerant Genetically Modified Oilseed Rape T45 for Food and Feed Uses, Import and Processing under Regulation (EC) No 1829/2003 (Application EFSA/GMO/UK/2005/25) 根据法规(EC) No 1829/2003(申请EFSA/GMO/UK/2005/25)对用于食品和饲料、进口和加工的耐草铵膦转基因油菜T45的环境风险评估
Pub Date : 2019-11-19 DOI: 10.9734/ejnfs/2019/v11i230140
A. Andreassen, P. Brandtzaeg, M. Finne, A. Holck, A. Jevnaker, O. Junttila, Heidi Sjursen Konestabo, R. Meadow, A. Mikalsen, K. Nielsen, M. Sanden, V. Sipinen, H. Opsahl-Sorteberg, R. Vikse
In preparation for a legal implementation of EU-regulation 1829/2003, the Norwegian Scientific Committee for Food Safety (VKM) has been requested by the Norwegian Directorate for Nature Management to conduct final environmental risk assessments for all genetically modified organisms (GMOs) and products containing or consisting of GMOs that are authorized in the European Union under Directive 2001/18/EC or Regulation 1829/2003/EC. The assignment includes a scientific environmental risk assessment of oilseed rape T45 (Reference EFSA/GMO/UK/2005/25) from Bayer CropScience for food and feed uses, import and processing. Oilseed rape T45 has previously been risk assessed by the VKM Panel on Genetically Modified Organisms (GMO), commissioned by the Norwegian Food Safety Authority related to the EFSAs public hearing in 2007 (VKM 2007a). Food additives produced from T45 oilseed rape were notified in the EU as existing food additives within the meaning of Article 8 (1)(b) of Regulation 1829/2003, authorized under Directive 89/10/EEC (Community Register 2005). Feed materials produced from T45 were also notified as existing feed products containing, consisting of or produced from T45 according to Articles 8 and 20 of Regulation (EC) No 1829/2003 in 2003.   A notification for placing on the market of T45 according to the Directive 2001/18/EC was submitted in March 2004 (C/GB/04/M5/4), covering import and processing of T45 into food and feed. The application was further transferred into Regulation (EC) No 1829/2003 in November 2005 (EFSA/GMO/UK/2005/25). An application for renewal of authorisation for continued marketing of food additives and feed materials produced from T45 oilseed rape was submitted under Regulation (EC) No 1829/2003 in 2007 (EFSA/GMO/RX/T45). The EFSA GMO Panel performed one single comprehensive risk assessment for all intended uses of genetically modified oilseed rape T45, and issued a comprehensive scientific opinion for both applications submitted under Regulation (EC) No 1829/2003. The scientific opinion was published in January 30 2008 (EFSA 2008), and food and feed products containing or produced from oilseed rape T45 was approved by Commission Decision 26 March 2009 (Commission Decision 2009/184/EC).  The oilseed rape T45 is however currently being phased out (EU-COM 2009). The commercialisation of T45 oilseed rape seeds in third countries was stopped after the 2005 planting season and stocks of all oilseed rape T45 lines have been recalled from distribution and destroyed. The applicant commits not to commercialize the event in the future and the import will therefore be restricted to adventitious levels in oilseed rape commodity. Thus the incidence of oilseed rape T45 in the EU is expected to be limited. The environmental risk assessment of the oilseed rape T45 is based on information provided by the notifier in the application EFSA/GMO/UK/2005/25 and EFSA/GMO/RX/T45, and scientific comments from EFSA and other member states mad
为了准备在法律上实施欧盟第1829/2003号法规,挪威自然管理局已要求挪威食品安全科学委员会(VKM)对欧盟根据第2001/18/EC号指令或第1829/2003/EC号法规授权的所有转基因生物(GMOs)和含有或由转基因生物组成的产品进行最后的环境风险评估。该任务包括拜耳作物科学公司对油菜籽T45(参考EFSA/GMO/UK/2005/25)进行科学的环境风险评估,用于食品和饲料用途、进口和加工。VKM转基因生物小组(GMO)此前已经对油菜T45进行了风险评估,该小组受挪威食品安全局委托,与2007年欧洲食品安全局的公开听证会有关(VKM 2007a)。根据指令89/10/EEC(共同体登记2005)授权,由T45油菜籽生产的食品添加剂在欧盟作为法规1829/2003第8 (1)(b)条意义上的现有食品添加剂进行通报。根据2003年法规(EC) No 1829/2003第8条和第20条,由T45生产的饲料材料也被通知为含有、由T45组成或由T45生产的现有饲料产品。2004年3月,根据指令2001/18/EC (C/GB/04/M5/4)提交了T45投放市场的通知,该通知涵盖了T45在食品和饲料中的进口和加工。该申请于2005年11月进一步转移到法规(EC) No 1829/2003 (EFSA/GMO/UK/2005/25)。2007年,根据法规(EC) No 1829/2003 (EFSA/GMO/RX/T45)提交了一份关于继续销售由T45油菜生产的食品添加剂和饲料材料的续期申请。欧洲食品安全局转基因生物小组对转基因油菜T45的所有预期用途进行了单一的全面风险评估,并对根据法规(EC) No 1829/2003提交的两项申请发表了全面的科学意见。该科学意见于2008年1月30日公布(EFSA 2008),委员会决定2009年3月26日批准了含有或由油菜T45制成的食品和饲料产品(委员会决定2009/184/EC)。然而,油菜T45目前正在逐步淘汰(EU-COM 2009)。2005年种植季后,T45油菜种子在第三国的商业化被停止,所有T45油菜品种的库存已从分销中召回并销毁。申请人承诺将来不会将该事件商业化,因此进口将被限制在油菜商品的未知水平。因此,油菜T45在欧盟的发病率预计是有限的。对油菜籽T45的环境风险评估是基于申请EFSA/GMO/UK/2005/25和EFSA/GMO/RX/T45中通报人提供的信息,以及欧洲食品安全局和其他成员国在欧洲食品安全局网站GMO Extranet上提供的科学评论。风险评估还考虑了其他同行评审的相关科学文献。VKM转基因生物专家组参照T45在欧洲经济区(EEA)的预期用途,根据《挪威食品法案》、《挪威基因技术法案》、《基因技术法案》、关于故意向环境释放转基因生物的指令2001/18/EC和转基因食品和饲料法规(EC) No 1829/2003中描述的原则,对T45进行了评估。挪威食品安全科学委员会还决定考虑欧洲食品安全局转基因植物及其衍生食品和饲料风险评估指南(EFSA 2006, 2011a)、转基因植物环境风险评估指南(EFSA 2010)、转基因植物风险评估比较指标的选择(EFSA 2011b)以及转基因植物上市后环境监测指南(EFSA 2006, 2011c)中所述的适当原则。油菜T45的科学风险评估包括插入DNA的分子特征和目标蛋白的表达,农艺和表型特征的比较评估,对植物适应性的意外影响,水平和垂直基因转移的潜力,以及上市后环境计划的评估。根据其授权,VKM强调,根据挪威基因技术法案和与基因技术法案相关的影响评估条例,可持续发展、社会效用和伦理考虑的评估不应由转基因生物小组进行。以普通油菜品种“AC Excel”原生质体为材料,通过农杆菌介导转化,获得了耐草铵膦油菜转化事件T45 (Unique identifier ACSBNØØ8-2)。 T45含有从病毒色链霉菌(Streptomyces viridochromogenes,菌株Tü 494)中分离的天然pat基因的合成版本。插入的基因编码磷酸钠丙氨酸乙酰转移酶(PAT),该酶对草甘膦铵具有耐受性。PAT酶通过将l-异构体乙酰化成n -乙酰- l-草铵解毒而不抑制谷氨酰胺合成酶,因此对除草剂具有耐受性。耐草铵膦油菜转化事件T45已被常规培育成一系列春型油菜品种。分子表征:分子表征数据表明,油菜基因组DNA中只有一个基因盒拷贝。对整合位点进行了适当的分析,包括插入DNA和侧翼区域的序列测定以及生物信息学分析。连接区域的生物信息学分析表明,没有任何潜在的新的orf编码已知毒素或过敏原。通过Southern分析,在基因组水平上证明了转化事件T45的遗传稳定性。分离分析表明,事件T45为显性单位点遗传性状。自1993年以来在加拿大种植的T45品系及其衍生品种对除草剂的稳定耐受性证实了表型稳定性。油菜转化事件T45和蛋白质的物理、化学和功能特性先前已由VKM转基因生物小组进行了评估,并认为令人满意(VKM 2007a)。比较评价:根据1995-1997年加拿大代表性地点和环境的田间试验数据的比较分析结果,得出结论:油菜T45在农艺和表型上与传统对应品种和商业可用参考品种相当,除了成熟度和PAT蛋白所带来的除草剂耐受性。田间评价支持这样一个结论,即与常规油菜相比,T45事件没有增加植物杂草/害虫潜力的表型变化。此外,结果表明,与常规油菜相比,草铵膦除草剂在作物中施用不会改变事件T45的表型和农艺性状。环境风险:据申请人称,T45事件已逐步淘汰,自2005年以来,所有T45系列油菜库存已从分销中召回并销毁。然而,由于不能完全排除未来种植和进口T45油菜籽进入欧盟/欧洲经济区的可能性,因此环境风险评估考虑了T45活籽在运输、储存、处理、加工和使用衍生产品过程中意外泄漏到环境中的风险。油菜主要是一种自花授粉的植物,但也有昆虫性的花,既能自花授粉,也能异花授粉。正常情况下异交率约为30%,但据报道异交率高达55%。与油菜有关的几种植物要么是栽培的,要么是在耕地和受干扰的土地上生长的杂草,要么是生长在可能引起油菜基因渗入的耕地之外。这些在芸苔属复合体和相关属中都有发现。科学文献报道了一系列油菜与相关分类群的控制杂交。由于染色体数目不匹配,大多数杂交种的生育能力严重降低。例外的是由油菜和野生萝卜(B. rapa ssp)杂交得到的杂种。在较小程度上,芥菜(B. juncea)已被证实在田间条件下自发杂交和转基因渗入。野生萝卜原产于挪威,是可耕地低地的一种常见杂草。没有证据表明,与传统的油菜品种相比,耐除草剂性状导致油菜T45或杂交野生近缘品种的适应性、持久性或入侵性增强,除非这些植物暴露于含有活性物质草铵膦的除草剂中。自2008年以来,含草铵膦除草剂已从挪威市场撤出,由于生殖毒性,该物质将于2017年在欧盟逐步淘汰。然而,随着时间的推移,在运输、储存、环境处理和加工成衍生产品过程中,可能会发生T45活籽的意外泄漏和损失,不能排除油菜T45小种群的建立。
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引用次数: 0
Environmental Risk Assessment of Glufosinate-Tolerant Genetically Modified Oilseed Rape MS8, RF3 and MS8 x RF3 for Import, Processing and Feed Uses under Directive 2001/18/EC (Notification C/BE/96/01) 根据指令2001/18/EC(通知C/BE/96/01)对用于进口、加工和饲料用途的耐草铵膦转基因油菜MS8、RF3和MS8 × RF3的环境风险评估
Pub Date : 2019-11-19 DOI: 10.9734/ejnfs/2019/v11i130130
A. Nerland, P. Brandtzaeg, M. Finne, A. Holck, O. Junttila, Heidi Sjursen Konestabo, R. Meadow, K. Nielsen, V. Sipinen, H. Opsahl-Sorteberg, R. Vikse, A. Andreassen
In preparation for a legal implementation of EU-regulation 1829/2003, the Norwegian Scientific Committee for Food Safety (VKM) has been requested by the Norwegian Directorate for Nature Management to conduct final environmental risk assessments for all genetically modified organisms (GMOs) and products containing or consisting of GMOs that are authorized in the European Union under Directive 2001/18/EC or Regulation 1829/2003/EC. The request covers scope(s) relevant to the Gene Technology Act. The request does not cover GMOs that VKM already has conducted its final risk assessments on. However, the Directorate requests VKM to consider whether updates or other changes to earlier submitted assessments are necessary.  The genetically modified, glufosinate-tolerant oilseed rape lines MS8, RF3 and MS8 x RF3 (Notification C/BE/96/01) are approved under Directive 2001/18/EC for import and processing for feed and industrial purposes since 26 March 2007 (Commission Decision 2007/232/EC). In addition, processed oil from genetically modified oilseed rape derived from MS8, RF3 and MS8 x RF3 were notified as existing food according to Art. 5 of Regulation (EC) No 258/97 on novel foods and novel food ingredients in November 1999. Existing feed and feed products containing, consisting of or produced from MS8, RF3 and MS8 x RF3 were notified according to Articles 8 and 20 of Regulation (EC) No 1829/2003 and were placed on the market in January 2000.   An application for renewal of the authorisation for continued marketing of existing food, food ingredients and feed materials produced from MS8, RF3 and MS8 x RF3 was submitted within the framework of Regulation (EC) No 1829/2003 in June 2007 (EFSA/GMO/RX/MS8/RF3). In addition, an application covering food containing or consisting of, and food produced from or containing ingredients produced from oilseed rape MS8, RF3 and MS8 x RF3 (with the exception of processed oil) was delivered by Bayer CropScience in June 2010 (EFSA/GMO/BE/2010/81).  The VKM GMO Panel has previously issued a scientific opinion related to the notification C/BE/96/01 for the placing on the market of the oilseed rape lines for import, processing and feed uses (VKM 2008). The health and environmental risk assessment was commissioned by the Norwegian Directorate for Nature Management in connection with the national finalisation of the procedure of the notification C/BE/96/01 in 2008. Due to the publication of updated guidelines for environmental risk assessments of genetically modified plants and new scientific literature, the VKM GMO Panel has decided to deliver an updated environmental risk assessment of oilseed rape MS8, RF3 and MS8 x RF3.   A scientific opinion on an application for the placing on the market of MS8/RF3 for food containing or consisting of, and food produced from or containing ingredients produced from MS8/RF3 (with the exception of processed oil) (EFSA/GMO/BE/2010/81) have also been submitted by the VKM GMO Panel (VKM 2012
根据其授权,VKM强调,根据挪威基因技术法案和与基因技术法案相关的影响评估条例,可持续发展、社会效用和伦理考虑的评估不应由转基因生物小组进行。开发了转基因油菜品系MS8和RF3,为生产f1杂交种子(MS8 × RF3)提供授粉控制系统。油菜是一种既能自花授粉(70%)又能异花授粉(30%)的作物。因此,要从两个不同的亲本中产生杂交种,只需要一个确保异花授粉的系统。由于杂种优势,异花授粉植物比自花授粉的油菜产量更高。该杂交系统采用传粉控制系统,通过将土壤细菌解淀粉芽孢杆菌的barnase和barstar基因插入和表达到两个独立的转基因油菜品系中。雄性不育系MS8的巴纳酶基因编码核糖核酸酶肽(RNase),在花药发育过程中在绒毡层细胞中表达。RNase影响RNA水平,破坏正常细胞功能,阻止早期花药发育,导致花粉缺乏活力和雄性不育。育性恢复系RF3含有一个barstar基因,该基因编码一种核糖核酸酶抑制剂(barstar肽),在花药发育过程中仅在花粉绒毡层细胞中表达。该肽可特异性抑制MS8细胞系表达的Barnase RNase。RNase和核糖核酸酶抑制剂形成稳定的一对一复合体,其中RNase失活。结果,当来自RF3受体系的花粉与雄性不育系MS8杂交时,MS8 × RF3后代在花药绒毡层细胞中表达RNase抑制剂,从而使杂交植株发育正常的花药并恢复育性。MS8和RF3中的barnase和barstar基因均与吸湿链霉菌的bar基因相连。bar基因由一个植物启动子驱动,该启动子在植物的所有绿色组织中都有活性,并编码磷脂酰丙氨酸乙酰转移酶(PAT)。PAT酶灭活了非选择性除草剂草铵膦的活性成分——膦丙酸(PPT)。将bar基因转移到油菜植株上,作为离体选择和制种育种选择的标记。分子表征:采用常规杂交方法获得了油菜杂交种MS8xRF3。亲本系MS8和RF3在申请人提供的文件中有很好的描述,许多出版物支持他们的数据。MS8似乎包含所需T-DNA结构的完整副本,包括棒状和藤蔓酶基因。同样,事件RF3可能包含bar和barstar基因的完整拷贝,以及bar基因的第二个不完整的无功能拷贝。单个事件中的插入在杂种MS8xRF3中被保留下来,所需的性状在几代之间稳定地遗传。油菜MS8、RF3和MS8xRF3以及新表达蛋白的物理、化学和功能特性先前已由VKM转基因生物小组评估,并被认为是令人满意的(VKM 2008, 2012)。转基因生物小组认为,在油菜转化事件MS8、RF3和MS8xRF3中重组插入物的物理、化学和功能特性的特征是令人满意的。转基因生物小组尚未根据插入物的分子特征确定与转基因植物相关的任何新风险。比较评价:基于对欧洲和加拿大代表性地点和环境的田间试验数据的比较分析结果,得出结论:油菜MS8、RF3和MS8 × RF3在农艺和表型上与传统油菜相当,除了新表达的barnase、barstar和PAT蛋白。田间评价支持以下结论:与常规油菜相比,MS8、RF3和MS8 × RF3事件没有表型变化表明植物杂草/害虫潜力增加。此外,研究结果表明,与常规油菜品种相比,草铵膦除草剂在作物中施用不会改变MS8、RF3和MS8 × RF3的表型和农艺性状。环境风险:考虑到通知C/BE/96/01的范围,除种植目的外,环境风险评估仅限于MS8、RF3和MS8 × RF3的活种子在运输、储存、处理、加工和使用衍生产品过程中意外泄漏到环境中。 油菜主要是一种自花授粉的植物,但也有昆虫性的花,既能自花授粉,也能异花授粉。正常情况下异交率约为30%,但据报道异交率高达55%。与油菜有关的几种栽培植物,以栽培和非栽培杂草的形式出现
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引用次数: 1
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European Journal of Nutrition & Food Safety
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