Pub Date : 2022-02-21DOI: 10.1080/09670262.2021.2002950
Juliane Kretschmann, Anže Žerdoner Čalasan, Johanna Knechtel, P. M. Owsianny, E. Facher, M. Gottschling
ABSTRACT Character evolution in calcareous dinophytes and their relatives is poorly understood at present, particularly regarding the transformations of the epitheca. The precise taxonomic identity of Chimonodinium lomnickii var. (≡ Peridinium) wierzejskii is unclear, but two different arrangements of the epithecal plate pattern have been reported. We studied dinophyte material collected across Central Europe, including the type localities of Peridinium wierzejskii (Polish Tatra) and Peridinium lomnickii var. punctulatum (Berlin), and investigated the morphology of eight monoclonal strains in detail. We further obtained rRNA sequences of the strains, including 27 new GenBank entries, for a molecular phylogenetic study using a representative taxon sample of the Thoracosphaeroideae. Five ITS ribotypes could be distinguished in Chimonodinium, but neither the distribution nor morphological differentiation seems to correlate with these ribotypes. An evolutionary transformation of the epitheca is inferred from the phylogenetic tree, with the predominant plate pattern found in C. lomnickii var. wierzejskii exemplifying the derived character state. However, the rare epithecal plate pattern of C. lomnickii var. wierzejskii represents the ancestral character state today found in, for example, Peridinium and Scrippsiella. This is an indication of an atavism, of which only very few cases are known from the microbial domain. The name Peridinium wierzejskii is taxonomically clarified by epitypification. HIGHLIGHTS • Rare documentation of atavism in the microbial domain• Deep morphology of strain established from type locality• Molecular phylogenetics inferred from concatenated rRNA sequence data
{"title":"Evolution of Thoracosphaeroideae (Peridiniales, Dinophyceae) and a case of atavism in taxonomically clarified Chimonodinium lomnickii var. wierzejskii from the Polish Tatra Mountains","authors":"Juliane Kretschmann, Anže Žerdoner Čalasan, Johanna Knechtel, P. M. Owsianny, E. Facher, M. Gottschling","doi":"10.1080/09670262.2021.2002950","DOIUrl":"https://doi.org/10.1080/09670262.2021.2002950","url":null,"abstract":"ABSTRACT Character evolution in calcareous dinophytes and their relatives is poorly understood at present, particularly regarding the transformations of the epitheca. The precise taxonomic identity of Chimonodinium lomnickii var. (≡ Peridinium) wierzejskii is unclear, but two different arrangements of the epithecal plate pattern have been reported. We studied dinophyte material collected across Central Europe, including the type localities of Peridinium wierzejskii (Polish Tatra) and Peridinium lomnickii var. punctulatum (Berlin), and investigated the morphology of eight monoclonal strains in detail. We further obtained rRNA sequences of the strains, including 27 new GenBank entries, for a molecular phylogenetic study using a representative taxon sample of the Thoracosphaeroideae. Five ITS ribotypes could be distinguished in Chimonodinium, but neither the distribution nor morphological differentiation seems to correlate with these ribotypes. An evolutionary transformation of the epitheca is inferred from the phylogenetic tree, with the predominant plate pattern found in C. lomnickii var. wierzejskii exemplifying the derived character state. However, the rare epithecal plate pattern of C. lomnickii var. wierzejskii represents the ancestral character state today found in, for example, Peridinium and Scrippsiella. This is an indication of an atavism, of which only very few cases are known from the microbial domain. The name Peridinium wierzejskii is taxonomically clarified by epitypification. HIGHLIGHTS • Rare documentation of atavism in the microbial domain• Deep morphology of strain established from type locality• Molecular phylogenetics inferred from concatenated rRNA sequence data","PeriodicalId":12032,"journal":{"name":"European Journal of Phycology","volume":null,"pages":null},"PeriodicalIF":2.4,"publicationDate":"2022-02-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46105077","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-02-21DOI: 10.1080/09670262.2022.2027023
Laura Lagourgue, S. Gobin, Maële Brisset, Simon Vandenberghe, C. Bonneville, Thierry Jauffrais, Sylvette Van Wynsberge, C. Payri
ABSTRACT Ulva is a green macroalgal genus with rich species diversity and worldwide distribution. While current knowledge on Ulva diversity focuses on temperate regions, genetic and morphological data in tropical and subtropical areas are scarce and the species richness is not clearly defined. The genus is known for its bloom-forming ability that can induce green tides leading to severe environmental and economic damage. In the last two decades, several important blooms of Ulva spp. have occurred in New Caledonia, requiring further investigations to identify the species involved. As knowledge of New Caledonian Ulva diversity is limited, an update to the Ulva spp. inventory in the area is essential. Based on Ulva specimens collected throughout New Caledonia (Grande Terre, Isle of Pines and Loyalty Islands), we (1) reassessed species diversity using species delimitation methods, (2) analysed morpho-anatomical characters to identify species and/or enrich their diagnosis, and (3) reconstructed a multilocus phylogeny (ITS, rbcL, tufA) of the genus. We found 21 secondary species hypotheses (SSHs) among our dataset, from which five were successfully assigned to U. lactuca, U. ohnoi, U. tepida, U. meridionalis and U. taeniata. Ten SSHs were defined as new species for which we provided taxonomic description, and six other SSHs were singletons that will need to be data-enriched for better interpretation. Our concatenated multilocus matrix included 61 Ulva species. Of these, 15 species were found in New Caledonia and were moderately to strongly supported. Among the Ulva species found in New Caledonia, seven are known to be bloom-forming which highlights the need for strict regulation and regular monitoring of water quality, particularly in areas exposed to strong nutrient input where these species can form green tides. Highlights Ulva diversity in New Caledonia was reassessed with 15 species highlighted. Ten new species have been discovered in New Caledonia. Indigenous species caused recent green tides in New Caledonia.
{"title":"Ten new species of Ulva (Ulvophyceae, Chlorophyta) discovered in New Caledonia: genetic and morphological diversity, and bloom potential","authors":"Laura Lagourgue, S. Gobin, Maële Brisset, Simon Vandenberghe, C. Bonneville, Thierry Jauffrais, Sylvette Van Wynsberge, C. Payri","doi":"10.1080/09670262.2022.2027023","DOIUrl":"https://doi.org/10.1080/09670262.2022.2027023","url":null,"abstract":"ABSTRACT Ulva is a green macroalgal genus with rich species diversity and worldwide distribution. While current knowledge on Ulva diversity focuses on temperate regions, genetic and morphological data in tropical and subtropical areas are scarce and the species richness is not clearly defined. The genus is known for its bloom-forming ability that can induce green tides leading to severe environmental and economic damage. In the last two decades, several important blooms of Ulva spp. have occurred in New Caledonia, requiring further investigations to identify the species involved. As knowledge of New Caledonian Ulva diversity is limited, an update to the Ulva spp. inventory in the area is essential. Based on Ulva specimens collected throughout New Caledonia (Grande Terre, Isle of Pines and Loyalty Islands), we (1) reassessed species diversity using species delimitation methods, (2) analysed morpho-anatomical characters to identify species and/or enrich their diagnosis, and (3) reconstructed a multilocus phylogeny (ITS, rbcL, tufA) of the genus. We found 21 secondary species hypotheses (SSHs) among our dataset, from which five were successfully assigned to U. lactuca, U. ohnoi, U. tepida, U. meridionalis and U. taeniata. Ten SSHs were defined as new species for which we provided taxonomic description, and six other SSHs were singletons that will need to be data-enriched for better interpretation. Our concatenated multilocus matrix included 61 Ulva species. Of these, 15 species were found in New Caledonia and were moderately to strongly supported. Among the Ulva species found in New Caledonia, seven are known to be bloom-forming which highlights the need for strict regulation and regular monitoring of water quality, particularly in areas exposed to strong nutrient input where these species can form green tides. Highlights Ulva diversity in New Caledonia was reassessed with 15 species highlighted. Ten new species have been discovered in New Caledonia. Indigenous species caused recent green tides in New Caledonia.","PeriodicalId":12032,"journal":{"name":"European Journal of Phycology","volume":null,"pages":null},"PeriodicalIF":2.4,"publicationDate":"2022-02-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47191818","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-02-07DOI: 10.1080/09670262.2021.2016985
V. Ryabushko, E. Gureeva, S. Kapranov, N. I. Bobko, A. Prazukin, M. V. Nekhoroshev
Abstract Rare earth metals are widely used in various technologies, and their environmental impact needs to be assessed. Brown algae are recognized bioindicators of xenobiotic pollution in coastal marine areas, so we studied the rare earth element contents in short-lived branchlets of the two most abundant species of Cystoseira (C. barbata and C. crinita) on Black Sea coasts. The abundance of rare earth elements including scandium (Sc), yttrium (Y), lanthanum (La) and 14 lanthanides in the algae, seawater and sediments were determined using inductively coupled plasma mass spectrometry. The average contents of the prevalent elements in the algae decreased in the following order: Sc ≥ La > Europium (Eu) > Y > Neodymium (Nd) > Cerium (Ce). The factors affecting rare earth element content were age > locality > species of the alga. Most of the rare earth metals reached their maximum levels in branchlets > 5 month old, presumably associated with biosorption processes, whereas others (Eu, Terbium (Tb), Lutetium (Lu)) were most concentrated in 2–5 month old branchlets. In contrast to existing reports on the possible use of brown seaweeds for monitoring rare earth elements in coastal waters, the suitability of Cystoseira spp. branchlets, which have a 7 month life cycle, for short-term rare earth contamination monitoring was not confirmed in this study. Highlights Cystoseira spp. branchlets accumulate many rare earth elements with age. The dominant elements in Cystoseira spp. are Sc ≥ La> Eu > Y > Nd > Ce. The most significant factors affecting rare earth element content are age > locality > species.
稀土金属广泛应用于各种技术中，其环境影响需要进行评估。褐藻是沿海海域公认的外源污染的生物指标，因此我们研究了黑海沿岸最丰富的两种囊藻(C. barbata和C. crinita)短寿命小枝中的稀土元素含量。采用电感耦合等离子体质谱法测定了藻类、海水和沉积物中钪(Sc)、钇(Y)、镧(La)和14种镧系元素的丰度。藻类中主要元素的平均含量依次为:Sc≥La > Europium (Eu) > Y >钕(Nd) >铈(Ce)。影响稀土元素含量的因素有年龄、地点、藻类种类等。大多数稀土金属在5 ~ 5月龄的小枝中含量最高，可能与生物吸附过程有关，而其他稀土金属(Eu、Terbium (Tb)、Lutetium (Lu))在2 ~ 5月龄的小枝中含量最高。与已有的褐藻可能用于监测沿海水域稀土元素的报道不同，本研究未证实生命周期为7个月的Cystoseira spp.小枝是否适合用于短期稀土污染监测。随着年龄的增长，Cystoseira sp .的小枝会积累大量的稀土元素。Cystoseira的优势元素为Sc≥La b> Eu b> Y b> Nd b> Ce。影响稀土元素含量最显著的因素是年龄>、地点>、种类>。
{"title":"Rare earth elements in brown algae of the genus Cystoseira (Phaeophyceae) (Black Sea)","authors":"V. Ryabushko, E. Gureeva, S. Kapranov, N. I. Bobko, A. Prazukin, M. V. Nekhoroshev","doi":"10.1080/09670262.2021.2016985","DOIUrl":"https://doi.org/10.1080/09670262.2021.2016985","url":null,"abstract":"Abstract Rare earth metals are widely used in various technologies, and their environmental impact needs to be assessed. Brown algae are recognized bioindicators of xenobiotic pollution in coastal marine areas, so we studied the rare earth element contents in short-lived branchlets of the two most abundant species of Cystoseira (C. barbata and C. crinita) on Black Sea coasts. The abundance of rare earth elements including scandium (Sc), yttrium (Y), lanthanum (La) and 14 lanthanides in the algae, seawater and sediments were determined using inductively coupled plasma mass spectrometry. The average contents of the prevalent elements in the algae decreased in the following order: Sc ≥ La > Europium (Eu) > Y > Neodymium (Nd) > Cerium (Ce). The factors affecting rare earth element content were age > locality > species of the alga. Most of the rare earth metals reached their maximum levels in branchlets > 5 month old, presumably associated with biosorption processes, whereas others (Eu, Terbium (Tb), Lutetium (Lu)) were most concentrated in 2–5 month old branchlets. In contrast to existing reports on the possible use of brown seaweeds for monitoring rare earth elements in coastal waters, the suitability of Cystoseira spp. branchlets, which have a 7 month life cycle, for short-term rare earth contamination monitoring was not confirmed in this study. Highlights Cystoseira spp. branchlets accumulate many rare earth elements with age. The dominant elements in Cystoseira spp. are Sc ≥ La> Eu > Y > Nd > Ce. The most significant factors affecting rare earth element content are age > locality > species.","PeriodicalId":12032,"journal":{"name":"European Journal of Phycology","volume":null,"pages":null},"PeriodicalIF":2.4,"publicationDate":"2022-02-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"59529217","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-01-19DOI: 10.1080/09670262.2021.2014983
Cláudio F. Silva, G. Pearson, E. Serrão, I. Bartsch, N. Martins
ABSTRACT Kelp forests are important habitats in the strongly environmentally and seasonally variable Arctic. There is a critical lack of knowledge about how seasonal conditions and climate change scenarios influence survival and reproduction of kelp early life stages. To better understand the regulation of kelp life cycle processes in this harsh environment we focused on the physiological performance and reproductive success of early life stages in Alaria esculenta and Laminaria digitata from Kongsfjorden, Spitsbergen. Gametophyte growth and survival during Arctic winter and subsequent sporophyte recruitment under spring conditions were investigated. Winter conditions (2°C, complete darkness) halted gametophyte growth and prevented the onset of gametogenesis in both species. The gametophytes of L. digitata but not A. esculenta became fertile after returning to spring conditions, suggesting that sporogenesis, sexual reproduction and recruitment in A. esculenta must occur successively during summer/autumn while in L. digitata a new generation of sporophytes could develop from over-wintering gametophytes. The effects of simulated canopy shading (offering protection against extreme irradiance stress, particularly as sea ice retreats), present-day and projected Arctic summer seawater temperatures, and nutrient levels on gametophyte survival, fertility and sporophyte recruitment success were also investigated in both species. A. esculenta gametophytes had greater survival and reproductive success than L. digitata, except under very low light (simulating dense canopy). In contrast, shading was required for reproductive success in L. digitata gametophytes. Predicted summer temperatures of 9°C reduced sexual reproduction in both species. Interactions observed between these environmental drivers probably reflect species-specific seasonal patterns of survival and reproduction. These differences between kelp species in response to abiotic factors and light levels (simulated canopy shading) suggest that climate change could alter community structure in the Arctic through effects on sexual reproduction and sporophyte recruitment success. HIGHLIGHTS• Gametophytes were able to endure long periods of darkness.• Parental kelp canopy is key for gametophyte survival and recruitment.• Climate change may alter kelp recruitment patterns.
{"title":"Microscopic life stages of Arctic kelp differ in their resilience and reproductive output in response to Arctic seasonality","authors":"Cláudio F. Silva, G. Pearson, E. Serrão, I. Bartsch, N. Martins","doi":"10.1080/09670262.2021.2014983","DOIUrl":"https://doi.org/10.1080/09670262.2021.2014983","url":null,"abstract":"ABSTRACT Kelp forests are important habitats in the strongly environmentally and seasonally variable Arctic. There is a critical lack of knowledge about how seasonal conditions and climate change scenarios influence survival and reproduction of kelp early life stages. To better understand the regulation of kelp life cycle processes in this harsh environment we focused on the physiological performance and reproductive success of early life stages in Alaria esculenta and Laminaria digitata from Kongsfjorden, Spitsbergen. Gametophyte growth and survival during Arctic winter and subsequent sporophyte recruitment under spring conditions were investigated. Winter conditions (2°C, complete darkness) halted gametophyte growth and prevented the onset of gametogenesis in both species. The gametophytes of L. digitata but not A. esculenta became fertile after returning to spring conditions, suggesting that sporogenesis, sexual reproduction and recruitment in A. esculenta must occur successively during summer/autumn while in L. digitata a new generation of sporophytes could develop from over-wintering gametophytes. The effects of simulated canopy shading (offering protection against extreme irradiance stress, particularly as sea ice retreats), present-day and projected Arctic summer seawater temperatures, and nutrient levels on gametophyte survival, fertility and sporophyte recruitment success were also investigated in both species. A. esculenta gametophytes had greater survival and reproductive success than L. digitata, except under very low light (simulating dense canopy). In contrast, shading was required for reproductive success in L. digitata gametophytes. Predicted summer temperatures of 9°C reduced sexual reproduction in both species. Interactions observed between these environmental drivers probably reflect species-specific seasonal patterns of survival and reproduction. These differences between kelp species in response to abiotic factors and light levels (simulated canopy shading) suggest that climate change could alter community structure in the Arctic through effects on sexual reproduction and sporophyte recruitment success. HIGHLIGHTS• Gametophytes were able to endure long periods of darkness.• Parental kelp canopy is key for gametophyte survival and recruitment.• Climate change may alter kelp recruitment patterns.","PeriodicalId":12032,"journal":{"name":"European Journal of Phycology","volume":null,"pages":null},"PeriodicalIF":2.4,"publicationDate":"2022-01-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41877511","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-01-17DOI: 10.1080/09670262.2021.2014982
Kornelia Duchnik, J. Białczyk, E. Chrapusta-Srebrny, Ariel Kaminski, B. Bober
ABSTRACT The increase in frequency and prevalence of cylindrospermopsin (CYN)-producing cyanobacteria blooms have become a growing problem worldwide. Therefore, the long-term effects of CYN on the physiological processes of the macrophyte, Lemna trisulca, were examined. During a 5-day exposure to a range of CYN concentrations (0.5, 1 and 5 μg ml−1), the low levels of stress experienced by L. trisulca stimulated its growth rate and photosynthesis and led to an increase in the synthesis of photosynthetic pigments. In turn, it was shown that a 3-week exposure to the highest tested CYN concentration (5 μg ml−1) resulted in the inhibition of biomass accumulation (by 7.6%) compared with that of the control plants, but necrosis and chlorosis were not observed. A negative correlation with the concentration of carotenoids was also observed, which may suggest that the antioxidant mechanisms of L. trisulca are effective only up to a certain CYN concentration. An increase in protein content in the tissues of macrophytes treated with concentrations of CYN ranging from 0.5 to 5 μg ml−1 indicates that the toxin did not inhibit protein synthesis but increased the metabolism of L. trisulca, potentially to provide energy for defence. The toxin also affects the homeostasis of mineral ions in cells. This is the first description of the physiological response of L. trisulca to the long-term effects of a wide range of CYN concentrations.
{"title":"The long-term exposure of cyanotoxin, cylindrospermopsin, on the macrophyte Lemna trisulca","authors":"Kornelia Duchnik, J. Białczyk, E. Chrapusta-Srebrny, Ariel Kaminski, B. Bober","doi":"10.1080/09670262.2021.2014982","DOIUrl":"https://doi.org/10.1080/09670262.2021.2014982","url":null,"abstract":"ABSTRACT The increase in frequency and prevalence of cylindrospermopsin (CYN)-producing cyanobacteria blooms have become a growing problem worldwide. Therefore, the long-term effects of CYN on the physiological processes of the macrophyte, Lemna trisulca, were examined. During a 5-day exposure to a range of CYN concentrations (0.5, 1 and 5 μg ml−1), the low levels of stress experienced by L. trisulca stimulated its growth rate and photosynthesis and led to an increase in the synthesis of photosynthetic pigments. In turn, it was shown that a 3-week exposure to the highest tested CYN concentration (5 μg ml−1) resulted in the inhibition of biomass accumulation (by 7.6%) compared with that of the control plants, but necrosis and chlorosis were not observed. A negative correlation with the concentration of carotenoids was also observed, which may suggest that the antioxidant mechanisms of L. trisulca are effective only up to a certain CYN concentration. An increase in protein content in the tissues of macrophytes treated with concentrations of CYN ranging from 0.5 to 5 μg ml−1 indicates that the toxin did not inhibit protein synthesis but increased the metabolism of L. trisulca, potentially to provide energy for defence. The toxin also affects the homeostasis of mineral ions in cells. This is the first description of the physiological response of L. trisulca to the long-term effects of a wide range of CYN concentrations.","PeriodicalId":12032,"journal":{"name":"European Journal of Phycology","volume":null,"pages":null},"PeriodicalIF":2.4,"publicationDate":"2022-01-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49595433","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-01-12DOI: 10.1080/09670262.2021.2007420
A. Stanojković, Svatopluk Skoupý, P. Hašler, A. Poulíčková, P. Dvořák
ABSTRACT Despite the extensive diversity of bacteria and their importance to the fundamental functioning of terrestrial ecosystems, their distribution patterns are still not fully known. To fill the gap and further understand the biogeographic patterns in bacteria, we investigated the phylogeographic structure and the underlying drivers of diversification among populations of the cyanobacterium Microcoleus spp. The phylogenetic history was reconstructed using 16S rRNA genes and the 16S–23S internal transcribed spacer (ITS) of 495 Microcoleus spp. isolates. Ancestral area and state reconstruction was employed to investigate the distributional and ecological patterns within Microcoleus. Both isolation by distance and isolation by environment were tested with distance matrices analysis. The phylogenetic signal tests were conducted in order to assess the influence of the climatic preferences on the diversification of Microcoleus isolates. The distribution and phylogenetic diversification of Microcoleus are driven by both isolation by distance and environment, leading to at least 13 distinct lineages that could represent novel cyanobacterial species. Microcoleus spp. exhibited a distinct phylogeographic structure within the respective lineages. The ancestral area and state reconstruction revealed that Microcoleus most likely arose in Europe in terrestrial habitats. The phylogenetic signal showed that the phylogeny significantly affects the climatic preferences of Microcoleus strains. Geographic distance and contemporary climatic conditions play significant roles in shaping the distribution and diversification of Microcoleus. The observed patterns of distribution may shift in the future due to the impact of climate change. Highlights Microcoleus exhibited distinct phylogeographic structure within the respective lineages. Geographic and environmental heterogeneity affect Microcoleus distribution and diversification. Genetically distinct lineages coexist at the same site.
{"title":"Geography and climate drive the distribution and diversification of the cosmopolitan cyanobacterium Microcoleus (Oscillatoriales, Cyanobacteria)","authors":"A. Stanojković, Svatopluk Skoupý, P. Hašler, A. Poulíčková, P. Dvořák","doi":"10.1080/09670262.2021.2007420","DOIUrl":"https://doi.org/10.1080/09670262.2021.2007420","url":null,"abstract":"ABSTRACT Despite the extensive diversity of bacteria and their importance to the fundamental functioning of terrestrial ecosystems, their distribution patterns are still not fully known. To fill the gap and further understand the biogeographic patterns in bacteria, we investigated the phylogeographic structure and the underlying drivers of diversification among populations of the cyanobacterium Microcoleus spp. The phylogenetic history was reconstructed using 16S rRNA genes and the 16S–23S internal transcribed spacer (ITS) of 495 Microcoleus spp. isolates. Ancestral area and state reconstruction was employed to investigate the distributional and ecological patterns within Microcoleus. Both isolation by distance and isolation by environment were tested with distance matrices analysis. The phylogenetic signal tests were conducted in order to assess the influence of the climatic preferences on the diversification of Microcoleus isolates. The distribution and phylogenetic diversification of Microcoleus are driven by both isolation by distance and environment, leading to at least 13 distinct lineages that could represent novel cyanobacterial species. Microcoleus spp. exhibited a distinct phylogeographic structure within the respective lineages. The ancestral area and state reconstruction revealed that Microcoleus most likely arose in Europe in terrestrial habitats. The phylogenetic signal showed that the phylogeny significantly affects the climatic preferences of Microcoleus strains. Geographic distance and contemporary climatic conditions play significant roles in shaping the distribution and diversification of Microcoleus. The observed patterns of distribution may shift in the future due to the impact of climate change. Highlights Microcoleus exhibited distinct phylogeographic structure within the respective lineages. Geographic and environmental heterogeneity affect Microcoleus distribution and diversification. Genetically distinct lineages coexist at the same site.","PeriodicalId":12032,"journal":{"name":"European Journal of Phycology","volume":null,"pages":null},"PeriodicalIF":2.4,"publicationDate":"2022-01-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48274308","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-01-05DOI: 10.1080/09670262.2021.2003867
Maria Irisvalda Leal Gondim Cavalcanti, Patricia María González Sánchez, M. Fujii
ABSTRACT Stranded seaweeds are reported worldwide; along the Brazilian coast, the occurrence of beach-cast seaweeds is increasing. In this study the diversity, coverage and biomass of beach-cast seaweeds were compared on the north-eastern (NE) and south-eastern (SE) coasts of Brazil. In total 110 taxa were identified: 80 Rhodophyceae, 13 Phaeophyceae and 17 Chlorophyceae. While the NE coast had higher species richness, the SE beaches exhibited higher coverage and biomass, predominantly Phaeophytes within the Dictyotales. The identified taxa were attributed to five functional-form groups and their respective frequencies were calculated and analysed. In Brazil, corticated species displayed the highest frequency at all study sites, especially Emboaca Beach, whereas the corticated foliose group was more frequent on Candeias, Itaoca and Pontal Beaches. A permutational analysis of variance revealed significant differences in coverage and biomass of the macroalgal assemblages across beaches. A homogeneity of multivariate dispersions indicated that these parameters also differed significantly between the NE and SE coasts, with a marked dissimilarity between the beaches studied. Our results will contribute to a better understanding of the biodiversity and biomass of beach-cast seaweeds for possible future economic use (e.g. as fertilizer) in a region where local incomes are low.
{"title":"Comparison of the diversity and biomass of beach-cast seaweeds from NE and SE Brazil","authors":"Maria Irisvalda Leal Gondim Cavalcanti, Patricia María González Sánchez, M. Fujii","doi":"10.1080/09670262.2021.2003867","DOIUrl":"https://doi.org/10.1080/09670262.2021.2003867","url":null,"abstract":"ABSTRACT Stranded seaweeds are reported worldwide; along the Brazilian coast, the occurrence of beach-cast seaweeds is increasing. In this study the diversity, coverage and biomass of beach-cast seaweeds were compared on the north-eastern (NE) and south-eastern (SE) coasts of Brazil. In total 110 taxa were identified: 80 Rhodophyceae, 13 Phaeophyceae and 17 Chlorophyceae. While the NE coast had higher species richness, the SE beaches exhibited higher coverage and biomass, predominantly Phaeophytes within the Dictyotales. The identified taxa were attributed to five functional-form groups and their respective frequencies were calculated and analysed. In Brazil, corticated species displayed the highest frequency at all study sites, especially Emboaca Beach, whereas the corticated foliose group was more frequent on Candeias, Itaoca and Pontal Beaches. A permutational analysis of variance revealed significant differences in coverage and biomass of the macroalgal assemblages across beaches. A homogeneity of multivariate dispersions indicated that these parameters also differed significantly between the NE and SE coasts, with a marked dissimilarity between the beaches studied. Our results will contribute to a better understanding of the biodiversity and biomass of beach-cast seaweeds for possible future economic use (e.g. as fertilizer) in a region where local incomes are low.","PeriodicalId":12032,"journal":{"name":"European Journal of Phycology","volume":null,"pages":null},"PeriodicalIF":2.4,"publicationDate":"2022-01-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42318163","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-12-23DOI: 10.1080/09670262.2021.1995630
R. V. Barbosa, S. Halac, E. Schwindt, M. Tatián
Abstract The changes within and between seasons in phytoplankton composition and abundance determine the carbon biomass available for upper levels of the food web. Temporal changes in phytoplankton community and environmental parameters in a port in Puerto Madryn, Southwest Atlantic were analysed. During an annual period (2011–2012), samples of surface seawater were collected approximately monthly. We determined phytoplankton community structure (species composition and abundance) and biomass (determined by carbon content and chlorophyll a (Chl a)). Water temperature, salinity and transparency were measured when sampling the surface water and local meteorological data were considered. The main groups observed were diatoms (Bacillariophyta; the most abundant during the concentration peaks of Chl a), dinoflagellates (Dinophyta) and flagellates, which mainly included species of Cryptophyta and Chlorophyta. Diatoms exhibited blooms in March (summer–autumn) and September (spring), represented by Skeletonema costatum and Pseudo-nitzschia spp. respectively. Dinoflagellates contributed to the highest carbon biomass, with peaks in January (summer) and April (autumn), exemplified by Prorocentrum micans and Scrippsiella acuminata, respectively. Temporal differences in community composition were related to the seasonal changes in temperature, solar irradiance, precipitation, salinity and wind velocity. The environmental conditions seem not only to determine the species composition but also cell size distribution: nanoplanktonic (≤ 20 µm) species dominated mainly during late spring, summer and early winter while microplanktonic species (> 20 µm) during late winter and early spring. Our results showed within-season changes and show that not only Chl a, but also carbon content can be considered, as the former is a biased estimator of phytoplankton biomass. This study provides the first seasonally resolved estimation in the area of the carbon biomass available for upper levels of the food web and a necessary information for future scenarios prediction. HIGHLIGHTS• Two diatom blooms were followed by an increase in dinoflagellate abundance.• Annual environmental conditions drive the phytoplankton community structure.• First seasonally estimation of autotrophic biomass availability for Patagonian trophic web.
{"title":"Seasonality of phytoplankton community and dynamics of autotrophic carbon in a cold temperate port (Argentina)","authors":"R. V. Barbosa, S. Halac, E. Schwindt, M. Tatián","doi":"10.1080/09670262.2021.1995630","DOIUrl":"https://doi.org/10.1080/09670262.2021.1995630","url":null,"abstract":"Abstract The changes within and between seasons in phytoplankton composition and abundance determine the carbon biomass available for upper levels of the food web. Temporal changes in phytoplankton community and environmental parameters in a port in Puerto Madryn, Southwest Atlantic were analysed. During an annual period (2011–2012), samples of surface seawater were collected approximately monthly. We determined phytoplankton community structure (species composition and abundance) and biomass (determined by carbon content and chlorophyll a (Chl a)). Water temperature, salinity and transparency were measured when sampling the surface water and local meteorological data were considered. The main groups observed were diatoms (Bacillariophyta; the most abundant during the concentration peaks of Chl a), dinoflagellates (Dinophyta) and flagellates, which mainly included species of Cryptophyta and Chlorophyta. Diatoms exhibited blooms in March (summer–autumn) and September (spring), represented by Skeletonema costatum and Pseudo-nitzschia spp. respectively. Dinoflagellates contributed to the highest carbon biomass, with peaks in January (summer) and April (autumn), exemplified by Prorocentrum micans and Scrippsiella acuminata, respectively. Temporal differences in community composition were related to the seasonal changes in temperature, solar irradiance, precipitation, salinity and wind velocity. The environmental conditions seem not only to determine the species composition but also cell size distribution: nanoplanktonic (≤ 20 µm) species dominated mainly during late spring, summer and early winter while microplanktonic species (> 20 µm) during late winter and early spring. Our results showed within-season changes and show that not only Chl a, but also carbon content can be considered, as the former is a biased estimator of phytoplankton biomass. This study provides the first seasonally resolved estimation in the area of the carbon biomass available for upper levels of the food web and a necessary information for future scenarios prediction. HIGHLIGHTS• Two diatom blooms were followed by an increase in dinoflagellate abundance.• Annual environmental conditions drive the phytoplankton community structure.• First seasonally estimation of autotrophic biomass availability for Patagonian trophic web.","PeriodicalId":12032,"journal":{"name":"European Journal of Phycology","volume":null,"pages":null},"PeriodicalIF":2.4,"publicationDate":"2021-12-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48646134","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-12-09DOI: 10.1080/09670262.2021.1987527
R. Romanov, A. Nikulin, V. Nikulin, A. Gontcharov
Abstract European paddy fields harbour a morphologically and genetically unusual charophyte that is distant from any other European species. The new species, Chara oryzae from Mediterranean rice fields, was described using an integrated approach, including scanning electron microscopy and molecular phylogenetics. The combination of monoecy, a triplostichous stem cortex with solitary spine cells, haplostephanous stipulodes, abbreviated branchlet segments with their mostly incomplete diplostichous cortication, and long undifferentiated part of a branchlet distinguish C. oryzae from other Chara species described to date. A unique set of phenotypic characteristics and lack of affinity to Chara sections and subsections prompted us to accommodate the species in a new section Corillionia of the subgenus Chara. C. oryzae could be recognized as a species non-native to Europe because it is known only in rice fields, which have been maintained for centuries in the region studied. HIGHLIGHTS • European paddy fields harbour a charophyte with unusual morphology and genotype distant from any European species.• A new species, Chara oryzae, is described using an integrated approach.• A new section, Corillionia, of the subgenus Chara is proposed.
{"title":"New species Chara oryzae and a new section Corillionia of Chara (Charales, Charophyceae) from European Mediterranean rice fields","authors":"R. Romanov, A. Nikulin, V. Nikulin, A. Gontcharov","doi":"10.1080/09670262.2021.1987527","DOIUrl":"https://doi.org/10.1080/09670262.2021.1987527","url":null,"abstract":"Abstract European paddy fields harbour a morphologically and genetically unusual charophyte that is distant from any other European species. The new species, Chara oryzae from Mediterranean rice fields, was described using an integrated approach, including scanning electron microscopy and molecular phylogenetics. The combination of monoecy, a triplostichous stem cortex with solitary spine cells, haplostephanous stipulodes, abbreviated branchlet segments with their mostly incomplete diplostichous cortication, and long undifferentiated part of a branchlet distinguish C. oryzae from other Chara species described to date. A unique set of phenotypic characteristics and lack of affinity to Chara sections and subsections prompted us to accommodate the species in a new section Corillionia of the subgenus Chara. C. oryzae could be recognized as a species non-native to Europe because it is known only in rice fields, which have been maintained for centuries in the region studied. HIGHLIGHTS • European paddy fields harbour a charophyte with unusual morphology and genotype distant from any European species.• A new species, Chara oryzae, is described using an integrated approach.• A new section, Corillionia, of the subgenus Chara is proposed.","PeriodicalId":12032,"journal":{"name":"European Journal of Phycology","volume":null,"pages":null},"PeriodicalIF":2.4,"publicationDate":"2021-12-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43183889","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-11-30DOI: 10.1080/09670262.2021.1970234
J. Cañavate, C. Fernández-Díaz
Abstract Studies on the influence of salinity on microalgal lipids have focused mainly on chlorophyte species and knowledge about photosynthetic secondary endosymbionts is limited. Haptophytes exemplify this situation and represent an interesting model to advance knowledge about the high phylogenetic variability of microalgae in their response to salinity. The estuarine Pavlovophyceae Diacronema vlkianum underwent particular lipid class and fatty acid changes under the effect of salinity. Variability due to salinity was higher in the fatty acid profile compared with that of the lipid class profile. Triacylglycerols decreased with salinity in actively growing cells and increased only when salinity rose to 50 and cell growth ceased. Hypersalinity caused a slight, although significant, sulpholipid decrease and a 4-fold increase in phosphatidylglycerol, suggesting a key role for this phospholipid in salt tolerance. Conversely, the betaine lipid diacylglycerol hydroxymethyl-N,N,N-trimethyl-β-alanine was specifically decreased with high salinity. The fatty acids that decreased the most with salinity were 14:0, 16:1n-7, 20:4n-6, 20:5n-3 and 22:6n-3, while higher levels of 16:1n-9, 17:1, 18:2n-6 and 18:3n-3 were induced by high salinity. Despite fatty acid changes, the unsaturation index did not vary with salinity, but nutritional value of D. vlkianum decreased at a salinity of 50 due to a lower presence of 20-C polyunsaturated fatty acids. This pattern of lipid variation in D. vlkianum with salinity has not been described in any other microalgae. Although D. vlkianum grows within a wide salinity range, production of nutritional and functional lipids is more favourable at low salinity values.
{"title":"Salinity induces unique changes in lipid classes and fatty acids of the estuarine haptophyte Diacronema vlkianum","authors":"J. Cañavate, C. Fernández-Díaz","doi":"10.1080/09670262.2021.1970234","DOIUrl":"https://doi.org/10.1080/09670262.2021.1970234","url":null,"abstract":"Abstract Studies on the influence of salinity on microalgal lipids have focused mainly on chlorophyte species and knowledge about photosynthetic secondary endosymbionts is limited. Haptophytes exemplify this situation and represent an interesting model to advance knowledge about the high phylogenetic variability of microalgae in their response to salinity. The estuarine Pavlovophyceae Diacronema vlkianum underwent particular lipid class and fatty acid changes under the effect of salinity. Variability due to salinity was higher in the fatty acid profile compared with that of the lipid class profile. Triacylglycerols decreased with salinity in actively growing cells and increased only when salinity rose to 50 and cell growth ceased. Hypersalinity caused a slight, although significant, sulpholipid decrease and a 4-fold increase in phosphatidylglycerol, suggesting a key role for this phospholipid in salt tolerance. Conversely, the betaine lipid diacylglycerol hydroxymethyl-N,N,N-trimethyl-β-alanine was specifically decreased with high salinity. The fatty acids that decreased the most with salinity were 14:0, 16:1n-7, 20:4n-6, 20:5n-3 and 22:6n-3, while higher levels of 16:1n-9, 17:1, 18:2n-6 and 18:3n-3 were induced by high salinity. Despite fatty acid changes, the unsaturation index did not vary with salinity, but nutritional value of D. vlkianum decreased at a salinity of 50 due to a lower presence of 20-C polyunsaturated fatty acids. This pattern of lipid variation in D. vlkianum with salinity has not been described in any other microalgae. Although D. vlkianum grows within a wide salinity range, production of nutritional and functional lipids is more favourable at low salinity values.","PeriodicalId":12032,"journal":{"name":"European Journal of Phycology","volume":null,"pages":null},"PeriodicalIF":2.4,"publicationDate":"2021-11-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42271448","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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