Pub Date : 2022-12-31DOI: 10.23937/2572-4045.1510066
Flores Arnulfo Calixto, Pérez Adriana Graciela Zumba, Jiménez Guillermo Meza, Santiago José Cruz, Meza Miguel Ángel Flores
{"title":"Transanesthetic Variables that Significantly Influence the Optimal Decrease in Creatinine in Kidney Transplant","authors":"Flores Arnulfo Calixto, Pérez Adriana Graciela Zumba, Jiménez Guillermo Meza, Santiago José Cruz, Meza Miguel Ángel Flores","doi":"10.23937/2572-4045.1510066","DOIUrl":"https://doi.org/10.23937/2572-4045.1510066","url":null,"abstract":"","PeriodicalId":120880,"journal":{"name":"International Journal of Transplantation Research and Medicine","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130834636","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-04-30DOI: 10.23937/2572-4045.1510064
Domínguez-Massa Carlos, Briz-Echeverría Paulina M, Tébar-Botí Eduardo, Heredia-Cambra Tomás, Sirgo-González Javier, López-Vilella Raquel, Pérez-Guillén Manuel, Almenar-Bonet Luis, Martínez-León Juan B
Primary cardiac tumors are rare, and most are benign. Cardiac lymphangiomas are extremely rare, being most frequently diagnosed casually in asymptomatic patients or upon complications derived from compression. As in most benign tumors, the treatment is resection. We report the case of a 69-year-old female who debuts with congestive heart failure and is then diagnosed with a giant unresectable lymphangioma. The literature regarding the treatment of this tumor is scarce, to the best of our knowledge, especially if right coronary artery involvement is present. When the tumor is considered unresectable, cardiac transplantation may be considered as treatment.
{"title":"Indication for Heart Transplantation in a Rare Giant Unresectable Cardiac Tumor","authors":"Domínguez-Massa Carlos, Briz-Echeverría Paulina M, Tébar-Botí Eduardo, Heredia-Cambra Tomás, Sirgo-González Javier, López-Vilella Raquel, Pérez-Guillén Manuel, Almenar-Bonet Luis, Martínez-León Juan B","doi":"10.23937/2572-4045.1510064","DOIUrl":"https://doi.org/10.23937/2572-4045.1510064","url":null,"abstract":"Primary cardiac tumors are rare, and most are benign. Cardiac lymphangiomas are extremely rare, being most frequently diagnosed casually in asymptomatic patients or upon complications derived from compression. As in most benign tumors, the treatment is resection. We report the case of a 69-year-old female who debuts with congestive heart failure and is then diagnosed with a giant unresectable lymphangioma. The literature regarding the treatment of this tumor is scarce, to the best of our knowledge, especially if right coronary artery involvement is present. When the tumor is considered unresectable, cardiac transplantation may be considered as treatment.","PeriodicalId":120880,"journal":{"name":"International Journal of Transplantation Research and Medicine","volume":"9 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128318584","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-12-31DOI: 10.23937/2572-4045.1510055
Jarmi Tambi, Khouzam Samir, Shekhar Nitika, White Launia, O HodgeDavid, L MaiMartin, M WadeiHani
Background: Improvement in short term outcomes after kidney transplant has been achieved by using different induction and maintenance therapeutic approaches. Long term outcomes have not matched the expectations of the transplant stakeholders. Our study aimed to address the early impact of induction agents on long term outcome of kidney transplant when measured by Iothalamate clearance. Methods: All adult kidney transplant recipients between January of 2012 and December of 2016 were reviewed. 649 patients were divided into three groups based on the induction agent (Basiliximab, Alemtuzumab, and Thymoglobulin). Protocoled 4 months and 48 months kidney allograft function evaluation with Iothalamate clearance test were compared among the three groups. Results: Patients who received Basiliximab were significantly older with no difference among African American and Caucasians. The 48 months assessment showed significant decline in median Iothalamate clearance in Basiliximab group at 49 ml/min vs. Alemtuzumab group 64.8 ml/min and Thymoglobulin 60 ml/min with p = 0.007. The Basiliximab group developed a significant higher proteinuria measured by spot urine to creatinine ratio at 48 months. Conclusions: The use of Basiliximab as an induction agent for kidney transplant is associated with significant decline in kidney function 5 years post-transplantation when measured by Iothalamate clearance.
背景:采用不同的诱导和维持治疗方法可以改善肾移植术后的短期预后。长期的结果并没有达到移植利益相关者的期望。我们的研究旨在解决诱导剂对肾移植长期结果的早期影响，通过测量Iothalamate清除率。方法:回顾性分析2012年1月至2016年12月接受肾移植的成人患者。649例患者根据诱导药物(Basiliximab、Alemtuzumab和Thymoglobulin)分为三组。比较三组患者术后4个月和48个月异体肾移植功能评价。结果:接受Basiliximab治疗的患者年龄明显大于非裔美国人和白种人，差异无统计学意义。48个月的评估显示，Basiliximab组Iothalamate中位清除率为49 ml/min，而Alemtuzumab组为64.8 ml/min, Thymoglobulin组为60 ml/min, p = 0.007。在48个月时，巴昔昔单抗组的尿蛋白与肌酐比值显著升高。结论:使用Basiliximab作为肾移植诱导剂与移植后5年的肾功能显著下降有关。
{"title":"The Impact of Different Induction Immunosuppressive Therapy on Long Term Kidney Transplant Function When Measured by Iothalamate Clearance","authors":"Jarmi Tambi, Khouzam Samir, Shekhar Nitika, White Launia, O HodgeDavid, L MaiMartin, M WadeiHani","doi":"10.23937/2572-4045.1510055","DOIUrl":"https://doi.org/10.23937/2572-4045.1510055","url":null,"abstract":"Background: Improvement in short term outcomes after kidney transplant has been achieved by using different induction and maintenance therapeutic approaches. Long term outcomes have not matched the expectations of the transplant stakeholders. Our study aimed to address the early impact of induction agents on long term outcome of kidney transplant when measured by Iothalamate clearance. Methods: All adult kidney transplant recipients between January of 2012 and December of 2016 were reviewed. 649 patients were divided into three groups based on the induction agent (Basiliximab, Alemtuzumab, and Thymoglobulin). Protocoled 4 months and 48 months kidney allograft function evaluation with Iothalamate clearance test were compared among the three groups. Results: Patients who received Basiliximab were significantly older with no difference among African American and Caucasians. The 48 months assessment showed significant decline in median Iothalamate clearance in Basiliximab group at 49 ml/min vs. Alemtuzumab group 64.8 ml/min and Thymoglobulin 60 ml/min with p = 0.007. The Basiliximab group developed a significant higher proteinuria measured by spot urine to creatinine ratio at 48 months. Conclusions: The use of Basiliximab as an induction agent for kidney transplant is associated with significant decline in kidney function 5 years post-transplantation when measured by Iothalamate clearance.","PeriodicalId":120880,"journal":{"name":"International Journal of Transplantation Research and Medicine","volume":"19 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2020-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130787804","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-05-18DOI: 10.23937/2572-4045.1510052
Mezquida Juan Pablo Reig, Bellorín Alilis Fontana, Ortega Alberto García, Francisco Gabriel Anguera de
{"title":"Covid-19 Pneumonia in Bilateral Lung Transplant Recipient: Case Report","authors":"Mezquida Juan Pablo Reig, Bellorín Alilis Fontana, Ortega Alberto García, Francisco Gabriel Anguera de","doi":"10.23937/2572-4045.1510052","DOIUrl":"https://doi.org/10.23937/2572-4045.1510052","url":null,"abstract":"","PeriodicalId":120880,"journal":{"name":"International Journal of Transplantation Research and Medicine","volume":"8 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2020-05-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"114265138","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-10-21DOI: 10.23937/2572-4045.1510045
Rodrigues Natacha, R. Filipe, S. Alice, Neves Pedro
The definition of Chronic Kidney Disease Mineral Bone Disorder (CKD-MBD) has suffered significant changes over the last decade as our knowledge on the matter grows. The complexity of this subject is even bigger if you consider renal transplant recipients (RTRs) they already have a legacy of CKD-MBD previous to transplant, their bones will be under the direct and indirect effects of immunosuppression and they will develop CKD-MBD secondary to their graft (dis) function. All these issues occur in addition to the risk factors of the general population for bone disease. Diagnosis and management of CKD-MBD in RTRs is difficult, several tools are yet to be validated and bone biopsy is not widely available. In the past years, new therapeutic strategies have been proposed for these patients. Special consideration should be given to the orthopedic management in RTRs regarding fracture risk and avascular necrosis of the major joints, particularly the femoral head, due to the effects of immunosuppression.
{"title":"The Bone in Renal Transplant Recipients-A Nephrological and Orthopedic Review","authors":"Rodrigues Natacha, R. Filipe, S. Alice, Neves Pedro","doi":"10.23937/2572-4045.1510045","DOIUrl":"https://doi.org/10.23937/2572-4045.1510045","url":null,"abstract":"The definition of Chronic Kidney Disease Mineral Bone Disorder (CKD-MBD) has suffered significant changes over the last decade as our knowledge on the matter grows. The complexity of this subject is even bigger if you consider renal transplant recipients (RTRs) they already have a legacy of CKD-MBD previous to transplant, their bones will be under the direct and indirect effects of immunosuppression and they will develop CKD-MBD secondary to their graft (dis) function. All these issues occur in addition to the risk factors of the general population for bone disease. Diagnosis and management of CKD-MBD in RTRs is difficult, several tools are yet to be validated and bone biopsy is not widely available. In the past years, new therapeutic strategies have been proposed for these patients. Special consideration should be given to the orthopedic management in RTRs regarding fracture risk and avascular necrosis of the major joints, particularly the femoral head, due to the effects of immunosuppression.","PeriodicalId":120880,"journal":{"name":"International Journal of Transplantation Research and Medicine","volume":"118 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-10-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128177843","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-04-29DOI: 10.23937/2572-4045.1510042
Custódio Reslem Feitosa, Mendes Matheus De Souza, Schwermann Maximilian Pinho, Brasil Ivelise Regina Canite, Pinho José Eudes Bastos, Leite Andrea Benevides
{"title":"Liver Transplant with a Donor after Bothropic Accident - Case Report","authors":"Custódio Reslem Feitosa, Mendes Matheus De Souza, Schwermann Maximilian Pinho, Brasil Ivelise Regina Canite, Pinho José Eudes Bastos, Leite Andrea Benevides","doi":"10.23937/2572-4045.1510042","DOIUrl":"https://doi.org/10.23937/2572-4045.1510042","url":null,"abstract":"","PeriodicalId":120880,"journal":{"name":"International Journal of Transplantation Research and Medicine","volume":"56 5","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"120921763","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-04-04DOI: 10.23937/2572-4045.1510041
Pedraza Morales
The donation of human tissues and organs increases significantly when tissue banks and organ transplant organizations work together in the procurement of human organs and tissues at donor sources (hospitals, coroners’ system, organ procurement agencies, and funeral homes, among others). To achieve this vital goal, national competent health authorities should consider the establishment of a mechanism that promotes the broadest possible cooperation between tissue banks and organ transplant organizations operating in the country with all available donor sources. One of the issues that can facilitate the above-mentioned cooperation is the adoption of all necessary laws and regulations to support the establishment of a national central office in charge of the procurement of human tissues and organs within the country, to consider the possibility of the establishment or designation of a national central office to act as regional office in charge of the coordination and cooperation among the different national offices established in different countries within a given region, and the adoption of a unified coding and traceability system that could identify all human tissues and organs used in transplant activities carried out in the country, or within the region, or at international level. The promotion of national, regional, and international cooperation between tissue banks and organ transplant organizations would enable the sharing of relevant information that could be important for medical practice and scientific studies carried out by many countries, particularly for those countries with a weak health care system.
{"title":"Some Proposals to Strengthen the Cooperation between Tissue Banks and Organ Transplant Organizations at National, Regional, and International Levels","authors":"Pedraza Morales","doi":"10.23937/2572-4045.1510041","DOIUrl":"https://doi.org/10.23937/2572-4045.1510041","url":null,"abstract":"The donation of human tissues and organs increases significantly when tissue banks and organ transplant organizations work together in the procurement of human organs and tissues at donor sources (hospitals, coroners’ system, organ procurement agencies, and funeral homes, among others). To achieve this vital goal, national competent health authorities should consider the establishment of a mechanism that promotes the broadest possible cooperation between tissue banks and organ transplant organizations operating in the country with all available donor sources. One of the issues that can facilitate the above-mentioned cooperation is the adoption of all necessary laws and regulations to support the establishment of a national central office in charge of the procurement of human tissues and organs within the country, to consider the possibility of the establishment or designation of a national central office to act as regional office in charge of the coordination and cooperation among the different national offices established in different countries within a given region, and the adoption of a unified coding and traceability system that could identify all human tissues and organs used in transplant activities carried out in the country, or within the region, or at international level. The promotion of national, regional, and international cooperation between tissue banks and organ transplant organizations would enable the sharing of relevant information that could be important for medical practice and scientific studies carried out by many countries, particularly for those countries with a weak health care system.","PeriodicalId":120880,"journal":{"name":"International Journal of Transplantation Research and Medicine","volume":"65 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-04-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126627885","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-03-11DOI: 10.23937/2572-4045.1510040
W. Hanna, Bander Marta, Bander Dorota, Biront Aleksandra, Zeair Samir, Wawrzynowicz-Syczewska Marta
Background: Vitamin D deficiency can cause many health problems and higher mortality. Chronic liver disease impairs vitamin D status by various mechanisms. The aim of our study was to estimate and directly compare vitamin D status in liver recipients before and within six months after LT to see whether there is an impact of restoration of proper liver function on 25(OH)D concentration. Patients and methods: Serum 25(OH)D concentration was determined and compared in the group of 110 adult patients before and within six months after LT. Measures performed right before transplantation were related to the etiology of liver disease, stage of cirrhosis and a season when the examination was done. 25(OH)D in the study group was also compared to the vitamin D concentration in the control group of 110 healthy persons matching the patients with respect to the age (p = 0.16), sex (p = 0.18) and body weight (p = 0.12). 25(OH)D concentration below 20 ng/mL was considered deficient, between 20 and 30 ng/ mL insufficient and > 30 ng/mL sufficient. Frequencies of some clinical episodes like fractures, infections, deaths and diabetes mellitus were compared between groups. Results: 25(OH)D concentration was significantly higher in the study group compared to the control group (20.83 + 13.48 vs. 14.8 + 8.39, p = 0.0001). There was a significant impact of summer-autumn season on better 25(OH)D concentration both in the study group and in the controls. The lowest concentration of vitamin D in the study group before LT was noted in alcoholic liver disease compared to the other etiologies (15.56 + 10.42 vs. 23.61 + 14.13, p = 0.002). The mean 25(OH)D concentration in the study group significantly improved after LT (27.37 + 12.5 vs. 20.83 + 13.48, p = 0.00001), but still more than 50% of recipients were significantly deficient. Conclusions: Vitamin D deficiency is ubiquitous. Liver insufficiency does not have much impact on vitamin D status. Patients with chronic liver disease as well as healthy subjects require regular vitamin D monitoring and supplementation when appropriate. Patients with alcoholic liver cirrhosis and the end-stage disease are in a special need. After LT concentration of vitamin D improves, but more than 50% of the recipients require either proper prophylaxis or treatment.
背景:维生素D缺乏会导致许多健康问题和更高的死亡率。慢性肝病通过多种机制损害维生素D的状态。我们研究的目的是估计和直接比较肝移植前和术后6个月内肝脏受体的维生素D状态，以了解肝功能恢复是否对25(OH)D浓度有影响。患者和方法:对110例成年患者在肝移植前和移植后6个月内的血清25(OH)D浓度进行测定和比较。移植前进行的测量与肝病的病因、肝硬化分期和检查时的季节有关。并将研究组的25(OH)D与对照组110名与患者年龄(p = 0.16)、性别(p = 0.18)、体重(p = 0.12)相匹配的健康人的维生素D浓度进行比较。25(OH)D浓度低于20 ng/mL为不足，20 ~ 30 ng/mL为不足，> 30 ng/mL为充足。比较两组间骨折、感染、死亡、糖尿病等临床事件的发生频率。结果:研究组25(OH)D浓度显著高于对照组(20.83 + 13.48 vs. 14.8 + 8.39, p = 0.0001)。在研究组和对照组中，夏秋季节对更好的25(OH)D浓度有显著影响。与其他病因相比，LT前研究组中酒精性肝病患者维生素D浓度最低(15.56 + 10.42 vs. 23.61 + 14.13, p = 0.002)。研究组25(OH)D的平均浓度在LT后明显改善(27.37 + 12.5 vs. 20.83 + 13.48, p = 0.00001)，但仍有超过50%的受体明显缺乏。结论:维生素D缺乏症普遍存在。肝功能不全对维生素D水平没有太大影响。慢性肝病患者和健康受试者需要定期监测维生素D，并在适当时补充维生素D。酒精性肝硬化和终末期肝病患者有特殊需要。肝移植后，维生素D浓度有所改善，但超过50%的接受者需要适当的预防或治疗。
{"title":"Vitamin D Status before and after Liver Transplantation","authors":"W. Hanna, Bander Marta, Bander Dorota, Biront Aleksandra, Zeair Samir, Wawrzynowicz-Syczewska Marta","doi":"10.23937/2572-4045.1510040","DOIUrl":"https://doi.org/10.23937/2572-4045.1510040","url":null,"abstract":"Background: Vitamin D deficiency can cause many health problems and higher mortality. Chronic liver disease impairs vitamin D status by various mechanisms. The aim of our study was to estimate and directly compare vitamin D status in liver recipients before and within six months after LT to see whether there is an impact of restoration of proper liver function on 25(OH)D concentration. Patients and methods: Serum 25(OH)D concentration was determined and compared in the group of 110 adult patients before and within six months after LT. Measures performed right before transplantation were related to the etiology of liver disease, stage of cirrhosis and a season when the examination was done. 25(OH)D in the study group was also compared to the vitamin D concentration in the control group of 110 healthy persons matching the patients with respect to the age (p = 0.16), sex (p = 0.18) and body weight (p = 0.12). 25(OH)D concentration below 20 ng/mL was considered deficient, between 20 and 30 ng/ mL insufficient and > 30 ng/mL sufficient. Frequencies of some clinical episodes like fractures, infections, deaths and diabetes mellitus were compared between groups. Results: 25(OH)D concentration was significantly higher in the study group compared to the control group (20.83 + 13.48 vs. 14.8 + 8.39, p = 0.0001). There was a significant impact of summer-autumn season on better 25(OH)D concentration both in the study group and in the controls. The lowest concentration of vitamin D in the study group before LT was noted in alcoholic liver disease compared to the other etiologies (15.56 + 10.42 vs. 23.61 + 14.13, p = 0.002). The mean 25(OH)D concentration in the study group significantly improved after LT (27.37 + 12.5 vs. 20.83 + 13.48, p = 0.00001), but still more than 50% of recipients were significantly deficient. Conclusions: Vitamin D deficiency is ubiquitous. Liver insufficiency does not have much impact on vitamin D status. Patients with chronic liver disease as well as healthy subjects require regular vitamin D monitoring and supplementation when appropriate. Patients with alcoholic liver cirrhosis and the end-stage disease are in a special need. After LT concentration of vitamin D improves, but more than 50% of the recipients require either proper prophylaxis or treatment.","PeriodicalId":120880,"journal":{"name":"International Journal of Transplantation Research and Medicine","volume":"4 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"124003178","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-03-07DOI: 10.23937/2572-4045.1510039
Mishra Mahendra Narain, T. David, D. Puja, Lal Vandana
Background: Prior to 2013, most centers in India performed only Complement Dependent Cytotoxicity crossmatch (CDCXM) for detection of donor specific antibodies. Aim: This study was undertaken to evaluate the usefulness of Luminex based crossmatch (LXM) for pretransplant detection of HLA class I and II donor specific IgG antibodies in patients with end stage renal disease. Methods: Luminex Crossmatch was performed on samples from 654 patients, which included 582 first time recipients and 72 retransplants. CDC crossmatch was done for all 654 recipients. Panel Reactive Antibody (PRA) screen was performed for 352 (53.8%) recipients. Low resolution HLA typing was done by reverse Sequence Specific Oligonucleotide probes on Luminex platform. Results: Twenty-five recipients (3.8%) had a positive CDCXM. LXM was positive in 277 samples (39.2%): HLA -class I donor specific antibodies (DSA) were detected in 40 (5.6%), class II DSA in 155 (21.9%) and both classes in 82 (11.5%) samples. High background was present in 70 (9.9%) samples the results of which were confirmed by retesting a fresh sample or by correlation with PRA screen. Conclusions: LXM in combination with PRA screen is useful for detection of clinically relevant HLA IgG donor specific antibodies for pretransplant workup and is a huge improvement on CDCXM.
{"title":"Luminex Crossmatch for Pre-Transplant Workup of Renal Transplants - 30 Months Experience from Indian Subcontinent","authors":"Mishra Mahendra Narain, T. David, D. Puja, Lal Vandana","doi":"10.23937/2572-4045.1510039","DOIUrl":"https://doi.org/10.23937/2572-4045.1510039","url":null,"abstract":"Background: Prior to 2013, most centers in India performed only Complement Dependent Cytotoxicity crossmatch (CDCXM) for detection of donor specific antibodies. Aim: This study was undertaken to evaluate the usefulness of Luminex based crossmatch (LXM) for pretransplant detection of HLA class I and II donor specific IgG antibodies in patients with end stage renal disease. Methods: Luminex Crossmatch was performed on samples from 654 patients, which included 582 first time recipients and 72 retransplants. CDC crossmatch was done for all 654 recipients. Panel Reactive Antibody (PRA) screen was performed for 352 (53.8%) recipients. Low resolution HLA typing was done by reverse Sequence Specific Oligonucleotide probes on Luminex platform. Results: Twenty-five recipients (3.8%) had a positive CDCXM. LXM was positive in 277 samples (39.2%): HLA -class I donor specific antibodies (DSA) were detected in 40 (5.6%), class II DSA in 155 (21.9%) and both classes in 82 (11.5%) samples. High background was present in 70 (9.9%) samples the results of which were confirmed by retesting a fresh sample or by correlation with PRA screen. Conclusions: LXM in combination with PRA screen is useful for detection of clinically relevant HLA IgG donor specific antibodies for pretransplant workup and is a huge improvement on CDCXM.","PeriodicalId":120880,"journal":{"name":"International Journal of Transplantation Research and Medicine","volume":"36 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-03-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"116512061","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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