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Inactivation of Byssochlamys fulva during ohmic heating of tomato juice. 在番茄汁的欧姆加热过程中灭活富勒虫。
IF 2.3 4区 农林科学 Q2 Engineering Pub Date : 2024-01-18 DOI: 10.1177/10820132231222509
Mahsa Mokhtari, Nafiseh Zamindar, Mohammadali Zia, Monir Doudi, Nafiseh Ghasemi Sepero

Ohmic heating is an emerging direct thermal technology, which uses electricity to heat food products volumetrically. Ohmic heating provides thermal and non-thermal effects like electropermeabilization to inactivate microorganisms. In this study, ohmic heating was used to inactivate Byssochlamys fulva in tomato juice. The main and interaction effects of initial pH (3.5 and 4.5) and voltage gradient (15 and 20 V/cm) were investigated on mold inactivation during ohmic heating at 88, 93, and 98 °C for 20, 10 and 5 min, respectively. The pH, acidity, total soluble solids, and Dvalue were compared. The results showed that pH and voltage gradient had significant effects on Dvalue and Zvalue (p < 0.05). In order to model the survival behavior of Byssochlamys fulva, due to the nonlinearity of the curves, Weibull model gave more accurate estimation compared to classical first-order model.

欧姆加热是一种新兴的直接加热技术,它利用电能对食品进行体积加热。欧姆加热具有热效应和非热效应,如电渗透灭活微生物。在这项研究中,欧姆加热技术被用来灭活番茄汁中的褐藻。在 88、93 和 98 °C 下分别加热 20、10 和 5 分钟,研究了初始 pH 值(3.5 和 4.5)和电压梯度(15 和 20 V/cm)对霉菌灭活的主要影响和交互影响。比较了 pH 值、酸度、总可溶性固形物和 D 值。结果表明,pH 值和电压梯度对 D 值和 Z 值(p Byssochlamys fulva,由于曲线的非线性,与经典的一阶模型相比,Weibull 模型能给出更准确的估计。
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引用次数: 0
Optimization of cold plasma processing conditions for fresh-cut kiwifruit slices by using response surface methodology. 利用响应面方法优化鲜切猕猴桃片的冷等离子加工条件
IF 2.3 4区 农林科学 Q2 Engineering Pub Date : 2024-01-18 DOI: 10.1177/10820132231225778
Yuan Tian, Wuqi Zhao, Zhenrong Liu, Mengke Jia, Qingan Zhang, Guitian Gao, Zhong Zhang

The work aims to optimize the process of cold plasma for fresh-cut kiwifruit. The effects of discharge times, treatment voltages, and slice thickness as well as the interaction between them were investigated. Factor analysis was used to screen out the characteristic indices of fresh-cut kiwifruit. Design-Expert software was used to design three-factor response surface tests and find the optimal parameters. The results revealed that the quality indices of fresh-cut kiwifruit were the color difference, brittleness, and solid-acid ratio, the established binomial regression equations were significant (P < 0.05). At the optimal level: 26 kV treatment voltage, 120 s discharge times, and 10 mm slice thickness, the optimized test values for the color difference, brittleness, solid-acid ratio and decreased logarithm value of total plate count were 2.25, 128.96 g·s, 18.03 and 2.30 lg(CFU·g-1), respectively. Cold plasma could significantly improve the inactivation of bacteria in fresh-cut kiwifruit.

这项研究旨在优化冷等离子体处理鲜切猕猴桃的工艺。研究了放电时间、处理电压和切片厚度的影响以及它们之间的相互作用。采用因子分析筛选出鲜切猕猴桃的特征指标。使用 Design-Expert 软件设计了三因素响应面试验,并找到了最佳参数。结果表明,鲜切猕猴桃的质量指标为色差、脆度和固酸比,建立的二项式回归方程显著(P )。在处理电压为 26 kV、放电时间为 120 s、切片厚度为 10 mm 的最佳水平下,色差、脆度、固酸比和总板数下降对数值的优化测试值分别为 2.25、128.96 g-s、18.03 和 2.30 lg(CFU-g-1)。冷等离子体能明显提高鲜切猕猴桃中细菌的灭活能力。
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引用次数: 0
Biofilm busters: Exploring the antimicrobial and antibiofilm properties of essential oils against Salmonella Enteritidis. 生物膜破坏者:探索精油对肠炎沙门氏菌的抗菌和抗生物膜特性。
IF 2.3 4区 农林科学 Q2 Engineering Pub Date : 2024-01-17 DOI: 10.1177/10820132241227004
Mariem Somrani, Juan-Pablo Huertas, Asunción Iguaz, Hajer Debbabi, Alfredo Palop

This study delves into an exploration of the antimicrobial and antibiofilm properties of the essential oils (EOs) of cinnamon, garlic, and onion on Salmonella Enteritidis. Firstly, disc diffusion and minimum inhibitory concentration (MIC) techniques were employed to assess the antibacterial activity of the EOs. Additionally, the study explored the effect of these EOs on both initial cell attachment and 24 h-preformed biofilms. The crystal violet assay was implemented to evaluate biofilm biomass. The findings revealed that cinnamon EO exhibited the highest anti-biofilm activity. Furthermore, initial cell attachment inhibition at MIC ranged between 50 and 65% for the three oils, while inhibition rates on preformed structures were lower than 40% for all EOs at this MIC concentration. The study also found that the effects of these oils were dosage- and time-dependent (p < 0.05), thereby urging the adoption of these natural extracts as effective strategies for combating Salmonella biofilms.

本研究探讨了肉桂、大蒜和洋葱精油(EOs)对肠炎沙门氏菌的抗菌和抗生物膜特性。首先,研究人员采用了盘扩散和最小抑菌浓度(MIC)技术来评估这些精油的抗菌活性。此外,研究还探讨了这些环氧乙烷对初始细胞附着和 24 小时后形成的生物膜的影响。采用水晶紫测定法来评估生物膜的生物量。研究结果表明,肉桂环氧乙烷的抗生物膜活性最高。此外,在 MIC 浓度下,三种精油的初始细胞附着抑制率介于 50% 和 65% 之间,而在此 MIC 浓度下,所有 EO 对预成结构的抑制率均低于 40%。研究还发现,这些精油的效果与剂量和时间有关(p 沙门氏菌生物膜。
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引用次数: 0
Evaluation of physicochemical, textural, and microbial characteristics of probiotic soy cheese during storage: Generation and isolation of bioactive peptides. 评估益生菌大豆奶酪在储存期间的物理化学、质地和微生物特性:生物活性肽的生成和分离。
IF 2.3 4区 农林科学 Q2 Engineering Pub Date : 2024-01-09 DOI: 10.1177/10820132231226257
Somayeh Mashayekh, Rezvan Pourahmad, Behrouz Akbari-Adergani, Mohammad Reza Eshaghi

The purpose of this study was to ascertain how probiotic culture affected the physicochemical, textural, and microbiological characteristics of probiotic soy cheese during storage. Moreover, the release of bioactive peptides during fermentation and storage was examined. Each cheese sample was made from one of the probiotic cultures of Lactobacillus acidophilus, Lacticaseibacillus casei, and Bifidobacterium lactis. Peptide extracts were prepared from these samples and fractionated using reversed-phase high-performance liquid chromatography. The sample containing L. acidophilus had the highest dry matter and hardness. The samples with L. acidophilus and B. lactis, respectively, had the highest concentrations of lactic acid and acetic acid. During storage the acidity, dry matter, lactic acid, acetic acid, and hardness of the samples increased but the pH, springiness, and cohesiveness reduced (P < 0.05). All samples had a probiotic count greater than 107 CFU/g at the end of the storage. Antibacterial and antioxidant properties were found in the peptide fractions that were extracted from the samples. T2F4 (the fourth fraction separated from L. casei sample) had the greatest functional properties. Sodium dodecyl sulphate-polyacrylamide gel electrophoresis revealed the existence of peptide with a molecular mass of 5-10 kDa. Therefore, produced cheese is regarded as a suitable source of potentially bioactive peptides which can be utilized in food industry.

本研究旨在确定益生菌培养如何影响益生菌大豆奶酪在储存过程中的物理化学、质地和微生物特性。此外,还研究了生物活性肽在发酵和储存过程中的释放情况。每个奶酪样品都是由嗜酸乳杆菌、酪酸乳杆菌和乳双歧杆菌中的一种益生菌培养物制成的。从这些样品中制备肽提取物,并使用反相高效液相色谱法进行分馏。含有嗜酸乳杆菌的样品干物质和硬度最高。分别含有嗜酸乳杆菌和乳酸杆菌的样品中乳酸和乙酸的浓度最高。在贮藏过程中,样品的酸度、干物质、乳酸、乙酸和硬度都有所上升,但 pH 值、回弹性和粘稠度都有所下降(贮藏结束时为 7 CFU/g)。从样品中提取的肽馏分具有抗菌和抗氧化特性。T2F4(从干酪乳杆菌样品中分离出的第四个馏分)具有最强的功能特性。十二烷基硫酸钠-聚丙烯酰胺凝胶电泳显示存在分子质量为 5-10 kDa 的肽。因此,生产的奶酪被认为是潜在生物活性肽的合适来源,可用于食品工业。
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引用次数: 0
Novel immobilization method of Lactiplantibacillus plantarum in grains for dry food products. 植物乳杆菌在谷物中的新型固定化方法,用于干制食品。
IF 2.3 4区 农林科学 Q2 Engineering Pub Date : 2024-01-05 DOI: 10.1177/10820132231224240
Marilia Silva Malvezzi Karwowski, Yasmine Sayuri Kassuia, Daniela Góes Turchenski, Ana Carolina Camargo de Oliveira Aust, Renata Ernlund Freitas de Macedo

This study aimed to assess the potential use of grains (amaranth, millet, and quinoa) as immobilizing matrices on the metabolic activity of Lactiplantibacillus plantarum and evaluate the viability of the probiotic immobilized in millet during simulated oro-gastrointestinal digestion (OGI) and storage. Firstly, different grains, sterilization and immobilization times were assessed. Secondly, Lp. plantarum was immobilized in millet and its viability and metabolic activity were assessed during non-refrigerated long-term storage and OGI. Metabolic activity was higher with 15 min. of grain sterilization and 72 h of immobilization. Lp. plantarum in millet showed the highest count. Millet grains were able to maintain the high population (>7 log CFU/g) and the metabolic activity of Lp. plantarum during up to 150 d of storage and conferred a protective effect on entrapped probiotic cells during OGI. Millet is a promising immobilizing matrix for the addition of probiotics in dry food products.

本研究旨在评估谷物(苋菜、小米和藜麦)作为固定基质对植物乳杆菌代谢活性的潜在影响,并在模拟口腔胃肠道消化(OGI)和储存过程中评估固定在小米中的益生菌的活力。首先,对不同的谷物、灭菌和固定时间进行了评估。其次,将植物乳杆菌固定在小米中,并评估其在非冷藏长期储存和 OGI 期间的活力和代谢活性。谷物灭菌 15 分钟和固定 72 小时后,代谢活性较高。小米中的 Lp.在长达 150 天的储藏期间,小米谷物能够维持植物胰蛋白酶的高数量(>7 log CFU/g)和代谢活性,并在 OGI 期间对夹带的益生菌细胞产生保护作用。小米是一种很有前景的固定基质,可用于在干制食品中添加益生菌。
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引用次数: 0
Stability of vitamin C, carotenoids, phenols, and antioxidant capacity of pasteurised orange juice with resistant maltodextrin storage. 抗麦芽糊精储存的巴氏消毒橙汁的维生素C、类胡萝卜素、酚类和抗氧化能力的稳定性。
IF 2.3 4区 农林科学 Q2 Engineering Pub Date : 2024-01-01 Epub Date: 2022-09-09 DOI: 10.1177/10820132221124200
Elías Arilla, Javier Martínez-Monzó, Pilar Codoñer-Franch, Purificación García-Segovia, Marta Igual

Resistant maltodextrin (RMD) was added at increasing concentrations (0%, 2.5%, 5% and 7.5%) before pasteurisation to orange juice to analyse its potential protective effect on the health-related bioactive compounds of pasteurised orange juice throughout its storage time. Samples were characterised in terms of basic physico-chemical properties and bioactive compounds at the beginning of the storage. Higher concentrations of RMD proved to better preserve the bioactive compounds of orange juice, thus obtaining a higher antioxidant capacity (AC). Stability of all samples was determined by measuring the same parameters at days 0, 15, 45, 75, 105, 136 and 170 of storage. °Brix and pH were very stable in all samples along storage, while all bioactive compouds had negative variations. However, RMD addition slightly improved ascorbic acid, vitamin C, total phenols, and total carotenoids retention, improving then its AC. This effect was greater in the 5% RMD-added samples. All bioactive compounds showed a positive Pearson's correlation coefficient with AC. Colour variations were also measured at days 105 and 170. All samples had a positive variation of all colour parameters, being this clearer at day 170. This work enlights the potential functionality of RMD to better preserve the health-related compounds of pasteurised orange juice.

在对橙汁进行巴氏消毒前,以递增浓度(0%、2.5%、5%和7.5%)添加耐药麦芽糊精(RMD),分析其在整个储存时间内对巴氏消毒后橙汁中与健康相关的生物活性化合物的潜在保护作用。在储存开始时,样品的基本物理化学性质和生物活性化合物被表征。较高浓度的RMD被证明可以更好地保存橙汁中的生物活性化合物,从而获得更高的抗氧化能力(AC)。所有样品的稳定性通过在储存第0、15、45、75、105、136和170天测量相同的参数来确定。在贮藏过程中,所有样品的白锐度和pH值都非常稳定,而所有生物活性化合物都有负变化。然而,添加RMD略微改善了抗坏血酸、维生素C、总酚和总类胡萝卜素的保留,提高了其AC,在添加5% RMD的样品中效果更大。所有生物活性化合物与AC的Pearson相关系数均为正。在第105天和第170天也测量了颜色变化。所有样品的所有颜色参数都有正变化,在第170天更明显。这项工作揭示了RMD的潜在功能,以更好地保存巴氏消毒橙汁中与健康相关的化合物。
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引用次数: 1
Pasteurization of Salmonella spp. in black fungus (Auricularia auricula) powder by radio frequency heating. 黑木耳(Auricularia auricula)粉末中沙门氏菌的射频加热巴氏杀菌。
IF 2.3 4区 农林科学 Q2 Engineering Pub Date : 2024-01-01 Epub Date: 2022-09-05 DOI: 10.1177/10820132221123437
Zheng Geng, Pengfei Ye, Liangfu Zhou, Hongfei Fu, Xiangwei Chen, Yequn Wang, Yunyang Wang

Radio frequency (RF) heating has been studied to inactivate bacteria in some powder foods. In this study, a 6 kW, 27.12 MHz RF system was used to pasteurize Salmonella in black fungus (Auricularia auricula) powder. The effects of different conditions (initial aw, electrodes gaps, particle sizes) on RF heating rate and uniformity were investigated. The results showed that RF heating rate was significantly (p < 0.05) improved with decreasing electrodes gap and increasing initial aw, and the heating rate was the slowest when the particle size was 120-160 mesh. However, these factors had no significant (p > 0.05) influence on heating uniformity. RF pasteurization of Salmonella in black fungus powder was also studied. The results showed that, to inactivate Salmonella for 5 log reductions in the cold spot (the center of surface layer), the time needed and bacteria heat resistance at designated temperature (65, 75, 85 °C) decreased with increasing aw, and the first order kinetics and Weibull model could be used to fit inactivation curves of Salmonella with well goodness. Quality analysis results showed that although RF pasteurization had no significant (p > 0.05) effect on Auricularia auricula polysaccharide (AAP) and total polyphenols, obvious changes were found on color. Results suggested that RF pasteurization can be considered as an effective pasteurization method for black fungus powder.

研究了射频(RF)加热灭活某些粉状食品中的细菌。本研究采用6kw、27.12 MHz射频系统对黑木耳粉末中的沙门氏菌进行巴氏杀菌。研究了不同条件(初始温度、电极间隙、颗粒尺寸)对射频加热速率和均匀性的影响。结果表明:随着电极间距的减小和初始aw的增大,射频加热速率显著提高(p < 0.05),且当粒径为120 ~ 160目时,射频加热速率最慢;但这些因素对加热均匀性的影响不显著(p > 0.05)。研究了黑木耳粉中沙门氏菌的射频巴氏灭菌。结果表明,在冷点(表层中心)降低5个对数时,在指定温度(65、75、85℃)下所需时间和细菌耐热性随温度的增加而降低,一级动力学和Weibull模型可以很好地拟合沙门氏菌的灭活曲线。质量分析结果显示,射频巴氏灭菌对黑木耳多糖(AAP)和总多酚含量无显著影响(p > 0.05),但对黑木耳颜色有明显影响。结果表明,射频巴氏杀菌是一种有效的黑木耳粉末巴氏杀菌方法。
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引用次数: 1
Sonication and thermal treatment of pineapple juice: Comparative assessment of the physicochemical properties, antioxidant activities and microbial inactivation. 菠萝汁的超声处理和热处理:理化性质、抗氧化活性和微生物灭活的比较评价。
IF 2.3 4区 农林科学 Q2 Engineering Pub Date : 2024-01-01 Epub Date: 2022-09-21 DOI: 10.1177/10820132221127504
Mominul Hoque, Suvro Talukdar, Keya Rani Roy, Mohammad Afzal Hossain, Wahidu Zzaman

Sonication is a novel processing method that is perceived as a substitute for heat treatment to ensure the safety of juice while reducing the loss of nutrients during processing. The impacts of sonication and thermal processing on pineapple juice, including physicochemical properties, antioxidant activities, and microbial inactivation, were studied. Pineapple juice was sonicated at 100 W and 140 W (for 5, 10 and 15 min) and heat-treated at 70 °C, 80 °C and 90 °C (for 1 min). Results indicated minor alterations in physicochemical characteristics after sonication and thermal processing, while sonication treatment showed better retention of the color, clarity, and browning index of pineapple juice. Moreover, sonication significantly increased the bioactive compounds (total carotenoids, ascorbic acid, phenolics, and flavonoids) and boosted the antioxidant activity of pineapple juice compared to the heat-processed juice. Although sonication reduced the microbial load significantly with the increasing exposure time, it could not thoroughly inactivate microorganisms like thermal treatment. Therefore, optimal sonication processing conditions are required to be determined. This study will help the food processors utilize sonication treatment in pineapple juice processing plants to inactivate microorganisms thoroughly while also boosting the nutritional value.

超声处理是一种新的加工方法,被认为是热处理的替代品,以确保果汁的安全,同时减少加工过程中营养物质的损失。研究了超声波和热处理对菠萝汁理化性质、抗氧化活性和微生物灭活的影响。菠萝汁在100 W和140 W下超声(5、10和15 min),在70℃、80℃和90℃下热处理(1 min)。结果表明,超声处理和热处理对菠萝汁的理化特性影响较小,超声处理对菠萝汁的颜色、透明度和褐变指数保持较好。此外,超声处理显著增加了菠萝汁的生物活性化合物(总类胡萝卜素、抗坏血酸、酚类物质和类黄酮),并提高了菠萝汁的抗氧化活性。虽然随着暴露时间的增加,超声处理能显著降低微生物负荷,但不能像热处理那样彻底灭活微生物。因此,需要确定最佳的超声处理条件。这项研究将有助于食品加工者在菠萝汁加工厂利用超声波处理彻底灭活微生物,同时提高营养价值。
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引用次数: 1
Effects of changes in homogenization sequence and temperature for milk on physicochemical properties of stirred yoghurt. 牛奶均质顺序和温度的变化对搅拌酸奶理化性质的影响。
IF 2.3 4区 农林科学 Q2 Engineering Pub Date : 2024-01-01 Epub Date: 2022-11-14 DOI: 10.1177/10820132221138827
Firuze Ergin, Ahmet Küçükçetin

In this study, the effects of homogenization sequence (before or after heat treatment) and homogenization temperature (40, 50 and 60 °C) for milk were investigated on physicochemical properties of stirred yoghurt. Stirred yoghurts were produced from homogenized and subsequently heat-treated milks, and heat-treated and subsequently homogenized milks and stored at 4 °C for 30 days. Number of grains, mean perimeter of grains, visual roughness, firmness, apparent viscosity, consistency coefficient in yoghurts produced from homogenized milk after heat treatment were higher, compared to those in yoghurts produced from milk homogenized before heat treatment, and these increased when homogenization temperature increased. To conclude, this study showed that some physicochemical properties of yoghurt were changed, by altering homogenization sequence and homogenization temperature for milk.

在本研究中,研究了牛奶的均质顺序(热处理前后)和均质温度(40、50和60℃)对搅拌酸奶理化性质的影响。搅拌酸奶是由均质后加热处理的牛奶和经过热处理后均质后在4℃下储存30天的牛奶制成的。热处理后均质乳生产的酸奶籽粒数、籽粒平均周长、视觉粗糙度、硬度、表观粘度、稠度系数均高于热处理前均质乳生产的酸奶,且随着均质温度的升高而升高。综上所述,本研究表明,通过改变牛奶的均质顺序和均质温度,可以改变酸奶的一些理化性质。
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引用次数: 1
Attenuating sugar spot and retaining quality of banana fruits by combined use of hot water and calcium lactate during storage. 热水与乳酸钙联用对香蕉果实贮藏过程中糖斑的抑制和品质的保持。
IF 2.3 4区 农林科学 Q2 Engineering Pub Date : 2024-01-01 Epub Date: 2022-10-19 DOI: 10.1177/10820132221132911
Myat Su-Mon, Ram Asrey, Nirmal Kumar Meena, Shruti Sethi

Combined use of hot water (HW) treatment and calcium lactate (CL) is a promising postharvest approach to preserve the food value and prolong the shelf life of fruits. The present experiment aims to determine the physiological loss in weight, firmness, respiration rate, ethylene and biochemical attributes of banana fruits treated with hot water (50 °C for 7 min) and aqueous CL dipping (1, 2, and 3% for 2 min). Treated fruits were stored under ambient conditions (22-25°C temperature and 60-65% of relative humidity) for up to 9 days. The study showed that combined use of HW and CL (3%) maintained higher hue angle, peel firmness (4.4 N), reduced decay loss (10.63%), respiration and ethylene evolution rate of stored fruits. Also, CL treatments (3%) with HW proved the best which reduced 6-fold sugar spot and 1.5-fold decay loss over untreated fruits. At the end of storage sensory parameters such as mouthfeel, peel colour and overall acceptability (score 6.9) were recorded higher in HW and CL 3% treated fruits. The findings indicated that pre-storage combined use of HW and CL has a great potential to preserve quality, delay ripening, and reduce sugar spots, and postharvest decay loss in banana fruit without any adverse effect on consumer appeal.

热水和乳酸钙联合处理是一种很有前途的水果采后保鲜和延长水果保质期的方法。本实验旨在测定香蕉果实经热水(50°C浸泡7 min)和水CL(1、2和3%浸泡2 min)处理后的重量、硬度、呼吸速率、乙烯和生化特性的生理损失。处理后的果实在环境条件下(温度22-25℃,相对湿度60-65%)保存9天。研究表明,3%的高水肥和低水肥可使贮藏果实保持较高的色相角、果皮硬度(4.4 N)、降低腐烂损失(10.63%)、呼吸和乙烯释放速率。与未处理的果实相比,加HW的CL(3%)处理效果最好,糖斑减少6倍,腐烂损失减少1.5倍。在储存结束时,感觉参数,如口感,果皮颜色和总体可接受性(得分6.9)在HW和CL 3%处理的水果中记录更高。结果表明,在香蕉果实贮藏前混合使用HW和CL,在保持果实品质、延缓成熟、减少糖斑和采后腐烂损失方面具有很大的潜力,且对消费者的吸引力没有不利影响。
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引用次数: 0
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