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Effect of dual physical modifications on structural and functional properties of gluten and whey protein: Ultrasound and microwave. 双重物理改性对谷蛋白和乳清蛋白结构和功能特性的影响:超声波和微波
IF 2.3 4区 农林科学 Q3 CHEMISTRY, APPLIED Pub Date : 2024-07-01 Epub Date: 2023-06-21 DOI: 10.1177/10820132231182099
Sayeh Mastani, Fereshte Bahmanyar, Saeedeh Shojaee-Aliabadi, Leila Mirmoghtadaie, Seyede Marzieh Hosseini

In this study, the effect of dual modification using ultrasound (100 and 300 W for 5, 10, and 15 min) and microwave (600 W for 45 s) treatments on functional properties of wheat gluten protein (WGP) and whey protein concentrate (WPC), as two by-products of food industry with different primary functional properties, was investigated. Ultrasound treatment did not affect the solubility of both proteins significantly but the emulsion and foam properties were increased up to 10 min. Nevertheless, microwave treatment after ultrasound caused a significant decrease in the solubility of both proteins. However, the foam stability of the WPC and WGP was not significantly modified after microwave treatment. The obtained results showed a more positive effect of ultrasound at 100 W for 10 min than other ultrasound treatments on the functional and structural properties of both proteins. The zeta potential of both proteins was decreased after dual physical modifications, but thermal stability of proteins was improved after microwave treatment.

小麦面筋蛋白(WGP)和浓缩乳清蛋白(WPC)是食品工业的两种副产品,具有不同的主要功能特性,本研究调查了使用超声波(100 和 300 瓦,5、10 和 15 分钟)和微波(600 瓦,45 秒)进行双重改性处理对其功能特性的影响。超声波处理对两种蛋白质的溶解度没有明显影响,但乳化和泡沫特性在 10 分钟内有所增加。然而,超声波处理后的微波处理会导致两种蛋白质的溶解度显著下降。不过,微波处理后 WPC 和 WGP 的泡沫稳定性并没有明显改变。结果表明,与其他超声波处理方法相比,100 瓦、10 分钟的超声波对两种蛋白质的功能和结构特性具有更积极的影响。经过双重物理改性后,两种蛋白质的 zeta 电位都有所下降,但微波处理后蛋白质的热稳定性有所提高。
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引用次数: 0
Fermented unripe tomato paste - Development of innovative salad dressings as a contribution to circular economy. 发酵的未成熟番茄酱--开发创新型沙拉酱,为循环经济做出贡献。
IF 2.3 4区 农林科学 Q3 CHEMISTRY, APPLIED Pub Date : 2024-07-01 Epub Date: 2022-12-13 DOI: 10.1177/10820132221144482
Sara Simões, Rafaela Santos, Isabel Sousa, Catarina Prista, Anabela Raymundo

As food trends evolve towards sustainable, healthy, and mildly processed products, it is essential to consider new food sources, which contribute to positive changes in the food industry and originate interesting new products. For the tomato industry, where only completely red tomatoes are used, unripe and non-red tomatoes constitute an important by-product, with important losses in the field of a perfectly edible vegetable food source. To give value to this unripe tomato, a fermentation with a consortium of lactic acid bacteria and yeast was previously optimized, originating an acidic pulp with optimized nutritional characteristics and great potential for salad dressing development. Pulp's texture was improved with two hydrocolloid systems: 0.5 g/100 g of xanthan gum; and a mixed system of xanthan gum and kappa-carrageenan (0.1 g/100 g each). After rheology optimization, spices and condiments were added to the thickened fermented pulp, and promising tasty sauces were obtained. These sauces were presented to a consumers' panel, with good acceptance. The production of these new healthy sauces aims to mitigate unripe tomato waste, adding value to the tomato industry by using a major industrial by-product.

随着食品趋势朝着可持续、健康和轻度加工产品的方向发展,有必要考虑新的食物来源,这有助于食品工业的积极变革,并产生有趣的新产品。番茄产业只使用完全红色的番茄,未成熟的非红色番茄是重要的副产品,在完全可食用的蔬菜食品来源领域造成了重大损失。为了提高这种未成熟番茄的价值,以前曾对乳酸菌和酵母联合体的发酵进行过优化,从而产生了一种酸性果肉,其营养特性得到了优化,并具有开发沙拉酱的巨大潜力。使用两种水胶体系统改善了果肉质地:黄原胶含量为 0.5 克/100 克;黄原胶和卡帕卡拉胶混合体系(各为 0.1 克/100 克)。流变优化后,在增稠的发酵果肉中加入香料和调味品,得到了美味可口的调味汁。这些调味汁被提交给一个消费者小组,获得了很好的认可。生产这些新型健康调味汁的目的是减少未成熟番茄的浪费,通过利用一种主要的工业副产品来增加番茄产业的价值。
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引用次数: 0
Exploring differences in the physicochemical and nutritional properties of mango flours and starches. 探索芒果粉和淀粉的理化和营养特性差异。
IF 2.3 4区 农林科学 Q3 CHEMISTRY, APPLIED Pub Date : 2024-06-10 DOI: 10.1177/10820132241259055
Nathalia Aparecida Barbosa Lossolli, Magali Leonel, Sarita Leonel, Maiqui Izidoro, Hebert Teixeira Cândido, Jaciene Lopes de Jesus Assis, Luciana Alves de Oliveira

Mangoes contain several components that are beneficial for health, in addition to being potential sources of starch for the food industry. However, a substantial amount of fruit is lost in the field because it does not meet commercial standards, resulting in food losses and environmental damage. Herein, the physicochemical properties of mango flours and starches obtained from different parts of the fruit of two cultivars were evaluated. Mango peel flours have higher levels of proteins, fibers, minerals, carotenoids, ascorbic acid, and antioxidant activity than pulp flours, in addition to a higher yellowing index and water and oil-holding capacity, and can be used as a functional flour. The pulp flours, with the higher starch content, showed characteristics that make them valuable as a potential ingredient in soft baking and gluten-free products. Mango starches have circular and oval shapes, with a bimodal distribution. All starches showed an A-type crystallinity pattern. Pulp starches showed a higher peak viscosity and breakdown, with a lower setback, and can be used as a thickening or gelling agent. The higher thermal stability of kernel starch suggests its application in sauces, baking, dairy products, and canned foods.

芒果含有多种有益健康的成分,此外还是食品工业潜在的淀粉来源。然而,由于不符合商业标准,大量果实在田间损失,造成食物损失和环境破坏。在此,我们对从两个栽培品种果实的不同部位获得的芒果粉和淀粉的理化特性进行了评估。与果肉粉相比,芒果皮粉的蛋白质、纤维、矿物质、类胡萝卜素、抗坏血酸和抗氧化活性含量更高,此外,黄变指数、持水和持油能力也更高,可用作功能性面粉。淀粉含量较高的果肉粉显示出的特性使其成为软烘焙和无麸质产品的潜在配料。芒果淀粉的形状有圆形和椭圆形,呈双峰分布。所有淀粉都呈现出 A 型结晶模式。果肉淀粉显示出更高的峰值粘度和分解度,具有较低的后退性,可用作增稠剂或胶凝剂。果仁淀粉具有较高的热稳定性,可用于调味汁、烘焙、乳制品和罐头食品。
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引用次数: 0
Applicability of wheat brewer's spent grain in steamed bread-making based on physicochemical and visual profiles assessment of doughs and breads. 根据面团和面包的理化和视觉特征评估小麦啤酒糟在馒头制作中的适用性。
IF 2.3 4区 农林科学 Q3 CHEMISTRY, APPLIED Pub Date : 2024-06-06 DOI: 10.1177/10820132241260453
Yuqi Zhang, Xueyan Wei, Jiao Bao, Kang Xu, Xiaoyan Chen, Mengmeng Guo

Brewer's spent grain (BSG), one of the main byproducts of brewing, has been widely used in the food industry due to its high nutritional components of dietary fiber, proteins, polysaccharides, and polyphenols. This study investigated the influence of wheat brewer's spent grain (WBSG) on the physicochemical properties of dough and steamed bread-making performance. The incorporation of WBSG in wheat flour significantly increased water absorption, development time, and degree of softening while decreasing the stability time of blending dough. Excessive WBSG up to 20% restricted the dough formation. WBSG contributed to the remarkable increase of pasting viscosities, pasting temperature, and immobilized water proportion in doughs. For all doughs, storage moduli (G') were higher than viscous moduli (G″). WBSG addition resulted in higher moduli values and the formation of highly networked gluten structure, finally leading to the lower specific volume, spread ratio, and elasticity of bread. Lightness (L*) of bread decreased with increasing WBSG while redness (a*) and total color difference (ΔE) augmented. Low WBSG addition (≤5%) could endow steamed bread with the appearance of a chocolate-like color and pleasant malt flavor, which is acceptable for most consumers. Nevertheless, the improvement of nutritional and functional characteristics of steamed bread incorporated with WBSG should be more focused in the future.

啤酒糟(BSG)是酿酒的主要副产品之一,因其含有较高的膳食纤维、蛋白质、多糖和多酚等营养成分而被广泛应用于食品工业。本研究探讨了小麦啤酒糟(WBSG)对面团理化性质和馒头制作性能的影响。在小麦粉中加入 WBSG 后,吸水率、发育时间和软化程度明显增加,同时降低了混合面团的稳定时间。过量的 WBSG(最高达 20%)限制了面团的形成。WBSG 显著提高了面团的糊化粘度、糊化温度和固定水比例。所有面团的储藏模量(G')都高于粘度模量(G″)。添加 WBSG 会导致模量值升高,并形成高度网络化的面筋结构,最终导致面包的比容、扩展率和弹性降低。面包的亮度(L*)随着 WBSG 的增加而降低,而红度(a*)和总色差(ΔE)则增加。低 WBSG 添加量(≤5%)可赋予馒头巧克力般的色泽和令人愉悦的麦芽风味,大多数消费者都能接受。不过,今后应更多地关注添加了 WBSG 的馒头的营养和功能特性的改善。
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引用次数: 0
Effect of the addition of black garlic on the quality parameters of jerked beef meat with pork. 添加黑蒜对猪肉手抓牛肉质量指标的影响。
IF 2.3 4区 农林科学 Q3 CHEMISTRY, APPLIED Pub Date : 2024-06-05 DOI: 10.1177/10820132241257280
Ranúsia Maria de Melo Lopes, Cristiani Viegas Brandão Grisi, Jorge Luiz Santos de Almeida, Janiele Ferreira da Silva, Camila Sampaio Mangolim, Solange de Sousa, Leonardo Augusto Fonseca Pascoal

The objective of this work was to evaluate the effects of the replacement of nitrite by natural antioxidants from black garlic (BG) on the quality parameters of jerked beef meat with pork for 60 days. Four formulations were prepared: control, 0.02% of sodium nitrite in brine curing, w/v (CON); 1.5% BG in brine curing, w/v (ASU); 1.5% BG in dry curing, w/w (ASS); and 1.5% of BG in the brine curing, w/v and 1.5% of BG in dry curing, w/w (ASUS). Nutritional composition, pH, water activity, shear force, fatty acid profile, color, and oxidative stability of the formulations were analyzed. The addition of BG did not affect the nutritional composition, pH, water activity, shear force, and fatty acid profile. On the other hand, it resulted in lower weight loss after centrifugation and lower values of L* and a*. TBARS values from the 30th day of storage were lower in the ASUS formulation, while carbonyl compounds at all times were lower than in the CON formulation. Results suggest that BG was an efficient alternative to nitrite in controlling protein oxidation during storage. Thus, the use of pork for the manufacture of jerked beef can be an alternative, and black garlic can be applied as a natural additive to the replacement of nitrite. In addition, black garlic was efficient in improving the oxidative stability of the jerked beef meat with pork.

这项工作的目的是评估用黑蒜(BG)中的天然抗氧化剂替代亚硝酸盐对腌制 60 天的猪肉牛肉质量参数的影响。制备了四种配方:对照组,0.02%的亚硝酸钠盐水腌制,w/v(CON);1.5%的黑蒜盐水腌制,w/v(ASU);1.5%的黑蒜干腌制,w/w(ASS);以及1.5%的黑蒜盐水腌制,w/v和1.5%的黑蒜干腌制,w/w(ASUS)。对配方的营养成分、pH 值、水活性、剪切力、脂肪酸组成、颜色和氧化稳定性进行了分析。添加 BG 不会影响营养成分、pH 值、水活性、剪切力和脂肪酸谱。另一方面,它降低了离心后的重量损失,降低了 L* 和 a* 值。ASUS 配方从储存第 30 天起的 TBARS 值较低,而羰基化合物在任何时候都低于 CON 配方。结果表明,在控制储存期间的蛋白质氧化方面,BG 是亚硝酸盐的有效替代品。因此,使用猪肉制作手抓牛肉可以作为一种替代品,而黑蒜可以作为天然添加剂替代亚硝酸盐。此外,黑蒜还能有效改善猪肉酱牛肉的氧化稳定性。
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引用次数: 0
Synergistic inhibition of Salmonella Typhimurium and Staphylococcus aureus in apple jam by cinnamaldehyde and potassium sorbate. 肉桂醛和山梨酸钾对苹果酱中鼠伤寒沙门氏菌和金黄色葡萄球菌的协同抑制作用。
IF 2.3 4区 农林科学 Q3 CHEMISTRY, APPLIED Pub Date : 2024-06-01 Epub Date: 2023-03-27 DOI: 10.1177/10820132231165541
Andreia Farias Pereira Batista, Daliah Alves Coelho Trevisan, Adriele Rodrigues Dos Santos, Alex Fiori Silva, Paula Aline Zanetti Campanerut-Sá, Benício Alves de Abreu Filho, Miguel Machinski Junior, Jane Martha Graton Mikcha

The objective of this study was to evaluate the antimicrobial effectiveness of cinnamaldehyde (CIN) and potassium sorbate (P.S.), alone and in combination, against Salmonella Typhimurium and Staphylococcus aureus in vitro and in apple jam. Antimicrobial activity in vitro was investigated by minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC), time-kill assay and determination of fractional inhibitory concentration index. CIN MIC and MBC was 312 μg/mL. P.S. MIC and MBC were 2500 and 5000 μg/mL, respectively, against S. Typhimurium; and 10,000 and 20,000 μg/mL, respectively, against S. aureus. The compounds combined exhibited a synergistic effect (FIC < 0.5), inhibiting S. Typhimurium growth after 12 h and S. aureus after 24 h. The effect of CIN and P.S., at sub-inhibitory concentrations, against bacterial strains in apple jam was evaluated during storage. Physicochemical and sensory analyses were also performed. No cultivable S. Typhimurium or S. aureus cells were recovered in apple jam supplemented with CIN + P.S. on the third day of storage. The addition of CIN and P.S. did not affect the physicochemical properties and sensory evaluation showed a score above 7.0. CIN and P.S. association at sub-inhibitory concentrations was effective in controlling foodborne pathogens and improved the shelf life of apple jam.

本研究的目的是评估肉桂醛(CIN)和山梨酸钾(P.S.)单独或混合使用在体外和苹果酱中对鼠伤寒沙门氏菌和金黄色葡萄球菌的抗菌效果。体外抗菌活性通过最低抑菌浓度(MIC)和最低杀菌浓度(MBC)、时间杀灭试验和分数抑菌浓度指数的测定进行了研究。CIN 的 MIC 和 MBC 为 312 μg/mL。P.S. 对伤寒杆菌的 MIC 和 MBC 分别为 2500 和 5000 μg/mL;对金黄色葡萄球菌的 MIC 和 MBC 分别为 10,000 和 20,000 μg/mL。这两种化合物结合使用可产生协同效应(12 个月后,金黄色葡萄球菌的生长速度为 FIC)。12 小时后伤寒杆菌的生长和 24 小时后金黄色葡萄球菌的生长。在苹果酱的储存过程中,对亚抑制浓度的 CIN 和 P.S. 对细菌菌株的作用进行了评估。此外还进行了理化和感官分析。在添加了 CIN + P.S. 的苹果酱中,储存第三天时没有发现可培养的伤寒杆菌或金黄色葡萄球菌细胞。添加 CIN 和 P.S. 不会影响果酱的理化特性,感官评估结果显示果酱得分高于 7.0。亚抑制浓度的 CIN 和 P.S. 可有效控制食源性致病菌,并延长苹果酱的保质期。
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引用次数: 0
Properties of insect protein concentrate and potential application in seasoned rice noodles. 昆虫浓缩蛋白的特性及在调味米粉中的潜在应用。
IF 2.3 4区 农林科学 Q3 CHEMISTRY, APPLIED Pub Date : 2024-06-01 Epub Date: 2022-12-13 DOI: 10.1177/10820132221144481
Chutima Thongkaew, Jittra Singthong, Sukanya Klangsinsirikul

In this study, we extracted proteins from four edible insect species (Acheta domesticus, Gryllus bimaculatus, Holotrichea sp., and Gryllotalpa orientalis), then evaluated their chemical and functional properties and used the extracts to prepare seasoned rice noodles. The four insect species contained 67-69% moisture, 13-18% protein, 6-10% fat, and 4-5% ash. The insect protein concentrate (IPC) contained 73-77% protein, and had high water solubility and emulsion stability (80-88%), moderate emulsion ability (56-65%), but low water- and oil-holding capacity (19% and 8-9%, respectively). The distinctive amino acid components in the IPC were glutamic acid and aspartic acid. In order to improve the nutritional and textural qualities of seasoned rice noodles, the IPC was added at rates of 0, 2, 4, 6, 8, and 10% by weight of rice flour. The results showed that the cooking weight was not significantly different from plain rice noodles; however, the 8% IPC fortified rice noodles had the highest tensile strength, whereas the lightness, yellowness, and redness decreased with an increase in insect protein content. This study demonstrates success in using 2-8% of IPC in rice noodles as a stabilizing or thickening agent.

在这项研究中,我们从四种可食用昆虫(Acheta domesticus、Gryllus bimaculatus、Holotrichea sp.和Gryllotalpa orientalis)中提取了蛋白质,然后评估了它们的化学和功能特性,并用提取物制备了调味米粉。这四种昆虫的水分含量为 67-69%,蛋白质含量为 13-18%,脂肪含量为 6-10%,灰分含量为 4-5%。昆虫蛋白浓缩物(IPC)的蛋白质含量为 73-77%,水溶性和乳化稳定性高(80-88%),乳化能力适中(56-65%),但持水量和持油量较低(分别为 19% 和 8-9%)。IPC 中独特的氨基酸成分是谷氨酸和天门冬氨酸。为了改善调味米粉的营养和质地,IPC 的添加量分别为米粉重量的 0、2、4、6、8 和 10%。结果表明,煮熟后的重量与普通米粉没有显著差异;然而,添加 8%IPC 的米粉具有最高的拉伸强度,而随着昆虫蛋白含量的增加,米粉的清淡度、黄度和红度都有所下降。这项研究表明,在米粉中添加 2 至 8% 的 IPC 作为稳定剂或增稠剂是成功的。
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引用次数: 0
Enhanced H2S biogenesis followed by its postharvest application retarded senescence development by promoting multiple antioxidant protection systems in button mushroom during cold storage. 在金针菇冷藏期间,通过促进多种抗氧化保护系统,增强 H2S 的生物生成,并在采后应用 H2S 可延缓衰老的发展。
IF 2.3 4区 农林科学 Q3 CHEMISTRY, APPLIED Pub Date : 2024-06-01 Epub Date: 2023-06-13 DOI: 10.1177/10820132221133144
Mahtab Moradi Digehsara, Rahim Naghshiband Hassani, Nasser Mahna, Silvana Nicola

Rapid postharvest quality deterioration of button mushroom as fruit body surface browning brings about senescence development and limits its distribution potential and postharvest storage. In this investigation, 0.5 mM of NaHS as the optimum concentration for H2S fumigation was applied to retain the quality of Agaricus bisporus mushrooms concerning some qualitative and biochemical attributes evaluation throughout 15 storage-day at 4 °C and 80-90% relative humidity. In H2S fumigated mushrooms, pileus browning index, weight loss and softening decreased, concomitant with higher cell membrane stability as revealed by subsidiary electrolyte leakage, malondialdehyde (MDA) and H2O2 contents compared to the control during the cold storage period. H2S fumigation boosted total phenolics, as presented by the enhanced phenylalanine ammonia-lyase (PAL) activity and total antioxidant scavenging activity, while polyphenol oxidase (PPO) activity diminished. Moreover, in H2S fumigated mushrooms not only peroxidase (POD), catalase (CAT), superoxide dismutase (SOD), glutathione reductase (GR) and glutathione peroxidase (GPx) activities but also ascorbic acid and glutathione (GSH) contents increased, even though glutathione (GSSG) content declined. The raised endogenous H2S level prompted by greater cystathionine ß-synthase (CBS), cystathionine ?-lyase (CSE), cysteine synthase (CS), L-cysteine desulfhydrases (LCD) and D-cysteine desulfhydrases (DCD) enzymes activities until 10d in fumigated mushrooms. In general, the increase in endogenous H2S biogenesis promoted by H2S fumigation in button mushrooms resulted in retarding senescence development, maintaining redox balance by boosting multiple enzymatic and non-enzymatic antioxidants defense parameters.

由于子实体表面褐变导致衰老,金针菇采后质量迅速恶化,限制了其销售潜力和采后贮藏。在这项研究中,采用 0.5 mM NaHS 作为 H2S 熏蒸的最佳浓度,在 4°C 和 80-90% 相对湿度条件下贮藏 15 天,以保持双孢蘑菇在一些质量和生化属性评估方面的品质。与对照组相比,经 H2S 熏蒸的蘑菇在冷藏期间的菌褶褐变指数、重量损失和软化程度降低,同时细胞膜稳定性提高,这体现在电解质泄漏、丙二醛(MDA)和 H2O2 含量降低。H2S 熏蒸提高了总酚类物质的含量,表现为苯丙氨酸氨解酶(PAL)活性和总抗氧化清除活性增强,而多酚氧化酶(PPO)活性降低。此外,在 H2S 熏蒸的蘑菇中,不仅过氧化物酶(POD)、过氧化氢酶(CAT)、超氧化物歧化酶(SOD)、谷胱甘肽还原酶(GR)和谷胱甘肽过氧化物酶(GPx)的活性提高了,抗坏血酸和谷胱甘肽(GSH)的含量也增加了,尽管谷胱甘肽(GSSG)的含量下降了。蘑菇熏蒸 10 天后,胱硫醚 ß-合成酶(CBS)、胱硫醚裂解酶(CSE)、半胱氨酸合成酶(CS)、L-半胱氨酸脱硫酶(LCD)和 D-半胱氨酸脱硫酶(DCD)的活性提高,从而导致内源性 H2S 水平升高。总的来说,H2S熏蒸促进了金针菇内源 H2S 生物生成的增加,从而延缓了衰老的发展,并通过提高多种酶和非酶抗氧化剂防御参数来维持氧化还原平衡。
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引用次数: 0
Phytochemical screening and antibacterial assessment of two macroalgae Ulva papenfussi and Ulva nematoidea (Chlorophyta) against the bacterium Vibrio parahaemolyticus. 针对副溶血性弧菌的两种大型藻类(叶绿藻)莼菜(Ulva papenfussi)和石莼(Ulva nematoidea)的植物化学筛选和抗菌评估
IF 1.8 4区 农林科学 Q3 CHEMISTRY, APPLIED Pub Date : 2024-06-01 Epub Date: 2023-03-27 DOI: 10.1177/10820132231165540
Jessie Vargas Cárdenas, Jorge Chávez Pérez, Nancy Martínez Ordinola, Ivan Soto Rodríguez, Luis Otavio Brito, Silvio Ricardo Maurano Peixoto, Alfredo Olivera Galvez

This study was conducted to determine whether the macroalgae Ulva papenfussi and Ulva nematoidea could be alternatives for preventing Litopenaeus vannamei vibriosis caused by the bacterium Vibrio parahaemolyticus. Phytochemical screening was performed on methanolic extracts to qualitatively determine the main groups of bioactive compounds, previous to an in vitro antibacterial test against V. parahaemolitycus. Phenols, polyphenols, flavonoids, and the high presence of carbohydrates were found in both macroalgae. U. papenfussi showed more presence of lipids and alkaloids than U. nematoidea. Macroalgae extracts prepared (v:v) with a 1:1 methanol: dichloromethane solvent was used for the in vitro test using the disc diffusion method (MDD). Filter paper discs impregnated with 1.0, 1.5, 2.0, 3.0, and 4.0 mg of the extracts showed antibacterial activity against V. Parahaemolitycus in a dose-dependent manner in both macroalgae. The inhibition zone varied significantly (p < 0.05) from 8.33 ± 0.12 to 11.41 ± 0.73 mm for 1 to 3 mg of extract levels, respectively. In conclusion, both macroalgae have antibacterial activity in their crude extracts against this bacteria. It is suggested to evaluate it as a feed additive for L. vannamei. This study is the first report on a phytochemical screening and antibacterial activity of these macroalgae against V. parahaemolyticus.

本研究旨在确定大型藻类石莼和针莼是否可作为预防副溶血性弧菌引起的万年青弧菌病的替代品。在对副溶血弧菌进行体外抗菌试验之前,对甲醇提取物进行了植物化学筛选,以确定生物活性化合物的主要类别。在两种大型藻类中都发现了酚类、多酚类、类黄酮以及大量碳水化合物。U. papenfussi 的脂类和生物碱含量高于 U. nematoidea。用 1:1 甲醇:二氯甲烷溶剂(v:v)制备的大型藻类提取物被用于体外试验,采用的是圆片扩散法(MDD)。浸渍了 1.0、1.5、2.0、3.0 和 4.0 毫克提取物的滤纸圆盘显示,两种大型藻类对副伞花叶病毒都具有抗菌活性,且呈剂量依赖性。抑制区的变化很大(p. vannamei)。本研究首次报道了这些大型藻类的植物化学筛选和对副溶血弧菌的抗菌活性。
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引用次数: 0
Chitosan-based nanocapsules by emulsification containing PUFA concentrates from tuna oil. 含有金枪鱼油中富含的 PUFA 浓缩物的乳化壳聚糖基纳米胶囊。
IF 2.3 4区 农林科学 Q3 CHEMISTRY, APPLIED Pub Date : 2024-06-01 Epub Date: 2023-01-26 DOI: 10.1177/10820132231153496
Jenifer Ines Engelmann, Bruna Silva de Farias, Andrei Vallerão Igansi, Patrick Peres Silva, Tito Roberto Santana Cadaval, Marcos Alexandre Gelesky, Valéria Terra Crexi, Luiz Antonio de Almeida Pinto

Chitosan nanocapsules containing polyunsaturated fatty acid (PUFA) concentrates from tuna oil, with EPA+DHA contents around 57% (w/w), were developed by emulsification process, using different chitosan concentration (1.0%, 1.5%, 2.0%, w/v) and stirring speed (10,000, 15,000, 20,000 rpm). The effects of these parameters on particle size and zeta potential were evaluated. The physical and oxidative stabilities were used to measure the product quality during storage. Chitosan concentration, stirring speed and its interaction significantly affected (p < 0.05) the particle size. In addition, chitosan concentration significantly affected (p < 0.05) the zeta potential of nanocapsules emulsion. Based on the results of physical and oxidative stabilities, the nanocapsules were stable for 30 days under refrigeration temperature (7 °C), and with 1.5-2% chitosan resulted in improved protection against oil oxidation. The nanocapsules produced with 2% chitosan and 10,000 rpm showed the lowest variations of polydispersity index and nanocapsules size after 30 days of storage (221.8 ± 3.0 nm). These conditions can be considered the most suitable to produce nanocapsules of PUFA concentrates from tuna oil using chitosan as wall material. These nanocapsules showed physical characteristics and oxidative stability, which could enable their application in the food industry, representing an important source of EPA and DHA fatty acids.

采用不同的壳聚糖浓度(1.0%、1.5%、2.0%,w v-1)和搅拌速度(10,000、15,000、20,000 rpm),通过乳化工艺制备了含有金枪鱼油浓缩多不饱和脂肪酸(PUFA)的壳聚糖纳米胶囊,其中 EPA 和 DHA 的含量约为 57% (w w-1)。评估了这些参数对粒度和 zeta 电位的影响。物理稳定性和氧化稳定性用于测量贮藏期间的产品质量。壳聚糖浓度、搅拌速度及其相互作用对产品的质量有明显影响(p p
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Food Science and Technology International
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