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Blending as a sample preparation protocol for mitigating intra- and inter-fruit heterogeneity in sensory analysis of sweetness and acidity in tomatoes. 在番茄甜度和酸度的感官分析中,将混合作为减轻果内和果间异质性的样品制备规程。
IF 1.8 4区 农林科学 Q3 CHEMISTRY, APPLIED Pub Date : 2024-11-25 DOI: 10.1177/10820132241300737
Joan Casals, Roser Romero Del Castillo

Tomato sensory analysis typically involves assessing different fruits, and the inherent intra- and inter-fruit variability poses experimental challenges. To address this, blending emerges as a sampling protocol to minimize panelist experimental error. This study delves into intra-(locule/pericarp) and inter-fruit heterogeneity, examining the efficacy of blended samples in assessing sweetness and acidity. Results reveal a higher acidity (22.2%) and sweetness perception (10.3%) in locular tissue, influenced by elevated titratable acids (TA) and soluble solids (TSS). The observed locular-to-fruit weight ratios (6-31%) might impact overall taste intensity. Fruit-to-fruit variation was high among the 16 varieties studied for TA, TSS, and dry matter. The use of blending to construct an "average" sample, increased sweetness (23%) and acidity perception (17%) without affecting sample ordering. Our results underscore the need to integrate locular relative weight into tomato phenotyping protocols and highlight the potential of blended samples in sensory analysis of traits related to the non-volatile fraction.

番茄感官分析通常涉及对不同果实的评估,而果实内部和果实之间固有的差异性给实验带来了挑战。为了解决这个问题,混合成为一种采样方案,以尽量减少小组成员的实验误差。本研究深入探讨了果实内部(果座/果皮)和果实之间的异质性,检验了混合样品在评估甜度和酸度方面的功效。结果显示,受可滴定酸(TA)和可溶性固形物(TSS)升高的影响,子房组织的酸度(22.2%)和甜度(10.3%)较高。观察到的子房与果实的重量比(6-31%)可能会影响整体口味强度。在研究的 16 个品种中,果与果间的可滴定酸、可溶性固形物和干物质差异很大。使用混合法制作 "平均 "样品,可提高甜度(23%)和酸度(17%),但不会影响样品排序。我们的研究结果表明,有必要将局部相对重量纳入番茄表型方案,并强调了混合样品在与非挥发性部分相关的性状感官分析中的潜力。
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引用次数: 0
Effect of essential oils on the antibacterial activity and technological properties of dark chocolate. 精油对黑巧克力抗菌活性和技术特性的影响
IF 1.8 4区 农林科学 Q3 CHEMISTRY, APPLIED Pub Date : 2024-11-22 DOI: 10.1177/10820132241300732
Julyana Laura Medeiros Vaughan, Lara Aguiar Borges, Maristela da Silva do Nascimento, Ana Paula Badan Ribeiro, Priscilla Efraim

Salmonella outbreaks associated with chocolate consumption are periodically reported. To address this issue, essential oils (EOs) have been proposed to be effective against foodborne pathogens. In addition, EOs constituents appear to modify the lipid phase of the product, leading to an improvement in quality. This study aimed to investigate the antimicrobial potential of EOs from Thymus vulgaris EO (TvEO) and the combination of T. vulgaris and Cymbopogon citratus EO (TvCcEO) in dark chocolate against S. Typhimurium ATCC 14028, as well as their influence on the technological properties during storage. The findings indicate that the EOs had no significant impact on the reduction of the target microorganism. Furthermore, the polymorphic transition from unstable crystals to the most stable form was detected in the chocolate control sample and chocolate with TvEO from 21 days of storage, while the transition in chocolate with TvCcEO was detected from 30 days of storage. Moreover, the addition of EOs reduced the chocolate's tensile strength and retarded the development of fat bloom from 62 days of storage. Therefore, these findings could prove valuable for future research investigating the potential of EOs in chocolate with the aim of delaying fat bloom development or inhibiting microbial growth.

与食用巧克力有关的沙门氏菌爆发事件时有报道。为了解决这个问题,有人提出精油(EO)可以有效抵抗食源性病原体。此外,精油成分似乎还能改变产品的脂相,从而提高产品质量。本研究旨在调查黑巧克力中的百里香环氧乙烷(TvEO)和百里香环氧乙烷与柠檬香茅环氧乙烷的组合(TvCcEO)对鼠伤寒杆菌 ATCC 14028 的抗菌潜力,以及它们在储存过程中对技术特性的影响。研究结果表明,环氧乙烷对目标微生物的减少没有显著影响。此外,在巧克力对照样品和添加了 TvEO 的巧克力中,从储存 21 天起就能检测到从不稳定性晶体到最稳定形态的多晶型转变,而添加了 TvCcEO 的巧克力从储存 30 天起就能检测到这种转变。此外,添加环氧乙烷降低了巧克力的拉伸强度,并延缓了储存 62 天后脂肪膨胀的发展。因此,这些发现对于今后研究 EO 在巧克力中的潜力,以延缓脂肪膨胀的发展或抑制微生物生长,具有重要价值。
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引用次数: 0
Preservation of strawberries using jellyfish gelatin-based biodegradable films and coatings under refrigerated storage. 使用基于水母明胶的生物可降解薄膜和涂层在冷藏条件下保存草莓。
IF 1.8 4区 农林科学 Q3 CHEMISTRY, APPLIED Pub Date : 2024-11-18 DOI: 10.1177/10820132241298487
Rasn Ranasinghe, Wli Wijesekara, S A Senanayake, Prd Perera, M M Pathmalal, Rauj Marapana

This study investigated the applicability of the biodegradable films and coatings prepared from gelatin extracted from jellyfish Acromitus flagellatus using two extraction methods; conventional hot water extraction and microwave-assisted extraction, for the preservation of strawberries under refrigerated storage (4 °C), compared to biodegradable films and coatings prepared from analytical grade cold water fish skin gelatin and porcine skin gelatin. After 21 days of storage, uncoated strawberries (control group) exhibited visual decay and fungal attacks while coated or film-wrapped strawberries exhibited a better appearance. Coating or wrapping with gelatin films significantly reduced the weight loss of strawberries and the changes in color, hardness, moisture content, pH, titratable acidity, and total soluble solids of coated/film-wrapped fruits during storage were minimal compared to the uncoated fruits. Of the treated groups, the weight loss of gelatin-coated strawberries was significantly lower compared to the fruits wrapped with corresponding gelatin films, in all gelatin types. Furthermore, strawberries coated or wrapped with jellyfish gelatin-based films exhibited comparable properties to the fruits that were coated or wrapped with other two types of gelatin films, indicating the potential use of gelatin extracted from jellyfish in biodegradable films or coating applications in the food industry.

本研究采用两种提取方法(传统热水提取法和微波辅助提取法),对从水母鞭毛藻中提取的明胶制备的可生物降解薄膜和涂层与用分析级冷水鱼皮明胶和猪皮明胶制备的可生物降解薄膜和涂层进行了比较,研究了可生物降解薄膜和涂层在冷藏(4 °C)条件下保存草莓的适用性。储藏 21 天后,无涂层草莓(对照组)出现视觉腐烂和真菌侵蚀,而有涂层或薄膜包裹的草莓外观更好。涂有明胶薄膜或用明胶薄膜包裹的草莓明显减少了重量损失,与未涂膜的水果相比,涂膜/薄膜包裹的水果在贮藏期间的颜色、硬度、水分含量、pH 值、可滴定酸度和总可溶性固形物的变化都很小。在所有明胶类型的处理组中,涂有明胶的草莓的重量损失明显低于用相应明胶薄膜包裹的水果。此外,涂有或包裹有海蜇明胶薄膜的草莓与涂有或包裹有其他两种明胶薄膜的水果表现出相似的特性,这表明从海蜇中提取的明胶可用于食品工业中的生物降解薄膜或涂层应用。
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引用次数: 0
Effect of silver nanoparticles in polyethylene packaging on physicochemical, microbiological, and textural properties of oil cake. 聚乙烯包装中的纳米银颗粒对油饼理化、微生物和质地特性的影响。
IF 1.8 4区 农林科学 Q3 CHEMISTRY, APPLIED Pub Date : 2024-11-15 DOI: 10.1177/10820132241297691
Monireh Joshaghanizade, Shahla Shahriari, Mozhdeh Karbalaee Hoseini Ghiasvand, Mania Salehifar

This study investigated silver nanoparticle (SNP)/titanium dioxide polyethylene films as chemical preservative substitutes to increase the shelf life of oil cake. The research examined three types of packaging films: standard low-density polyethylene (LDPE), LDPE with 3% SNP, and LDPE with 5% SNP, and assessed their impact on oil cake shelf life. Analysis of packaging films included scanning and transmission electron microscopy (SEM and TEM), and Fourier-transform infrared spectroscopy. The study evaluated the effects of SNP on chemical and microbial stability on oil cakes in different storage days (1st, 15th, 30th, and 37th days) at 25 °C. Parameters such as moisture content, water vapor permeability, water activity, peroxide, fat acidity, texture, and microorganisms were studied. The results demonstrated that SNP-containing films can extend the shelf life of oil cake up to 37 days, marked by a significant decrease in microbial growth, particularly mold, compared to conventional packaging films. Notably, films with 5% SNP concentrations exhibited the highest efficacy in preserving the quality of the oil cake. Higher concentrations of SNP led to an increased reduction in microbial load and enhanced mold control while maintaining favorable quality assessment indices throughout the storage period from day 1 to day 30. Remarkably, oil cake packaged with a 5% SNP polymer film displayed the most promising outcomes, extending shelf life up to 30 days at 25 °C.

本研究调查了纳米银(SNP)/二氧化钛聚乙烯薄膜作为化学防腐剂替代品对延长油饼保质期的影响。研究考察了三种包装膜:标准低密度聚乙烯(LDPE)、含 3% SNP 的低密度聚乙烯和含 5% SNP 的低密度聚乙烯,并评估了它们对油饼保质期的影响。对包装膜的分析包括扫描和透射电子显微镜(SEM 和 TEM)以及傅立叶变换红外光谱。研究评估了 SNP 在 25 °C、不同储存天数(第 1 天、第 15 天、第 30 天和第 37 天)下对油饼化学和微生物稳定性的影响。研究了水分含量、水蒸气渗透性、水活性、过氧化物、脂肪酸度、质地和微生物等参数。结果表明,与传统包装膜相比,含 SNP 的薄膜可将油饼的货架期延长至 37 天,其显著特点是微生物的生长,尤其是霉菌的生长。值得注意的是,5% SNP 浓度的薄膜在保持油饼质量方面的功效最高。SNP 浓度越高,微生物量减少越多,霉菌控制能力越强,同时在从第 1 天到第 30 天的整个储存期间都能保持良好的质量评估指数。值得注意的是,使用 5% SNP 聚合物薄膜包装的油饼显示出最有前途的结果,在 25 °C 下可延长货架期长达 30 天。
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引用次数: 0
Evaluation of the performance of low-fat (oil-fat) dressings based on chemically modified Guayabo plantain starch (Musa paradisiaca L.). 基于化学改性瓜亚博芭蕉淀粉(Musa paradisiaca L.)的低脂(油脂)敷料的性能评估。
IF 1.8 4区 农林科学 Q3 CHEMISTRY, APPLIED Pub Date : 2024-11-15 DOI: 10.1177/10820132241297741
Dayanna Alexandra Moreno-Ríos, Juan Carlos Lucas-Aguirre, Magda Ivonne Pinzón-Fandiño, Johan Sebastián Henao-Ossa

Guayabo plantain (GP) starch was chemically modified by acetylation to evaluate its role as a stabilizer and emulsifier in low-fat dressings. Native starch (NS) from GP was chemically modified starch (MS), and its functional properties, such as water absorption index, water solubility index, swelling power, gelatinization temperature (Tg), were evaluated. Additionally, functional groups and morphology were identified using Fourier transform infrared spectroscopy (FT-IR) and scanning electron microscopy. Low-fat dressings were prepared using NS and MS at two concentrations, 2% and 3% (NS2, NS3, MS2, MS3), and the stability of the dressings was evaluated over a storage period of 28 days at 4 °C ± 2.0 °C. The percentage of acetylation and the degree of substitution obtained were 2.48% and 0.01, respectively, complying with current regulations. MS showed a higher amylose content (23.62 ± 1.89%) than NS (16.01 ± 0.43%). The Tg of MS decreased, and the appearance of bands at 1012 and 1723 cm-1 in the FT-IR spectra suggested a modification in the functional characteristics of starch due to acetylation. Emulsions of MS at 2% and 3% (MS2 and MS3) showed a smaller droplet size and higher interfacial dispersion. However, MS3 had higher viscosity, which contributed to an increase in hydrophobicity and delays in flocculation and subsequent coalescence. This research study provides useful information on the use of 3% MS dressings in new food formulations, reducing fat content while preserving functional characteristics, thus ensuring greater stability.

对瓜亚博车前草(GP)淀粉进行乙酰化化学改性,以评估其在低脂敷料中作为稳定剂和乳化剂的作用。用化学改性淀粉(MS)对来自瓜亚博车前草(GP)的原生淀粉(NS)进行了化学改性,并对其吸水指数、水溶性指数、膨胀力、糊化温度(Tg)等功能特性进行了评估。此外,还使用傅立叶变换红外光谱(FT-IR)和扫描电子显微镜鉴定了功能基团和形态。使用 2% 和 3% 两种浓度的 NS 和 MS 制备了低脂敷料(NS2、NS3、MS2、MS3),并评估了敷料在 4 °C ± 2.0 °C 下储存 28 天的稳定性。乙酰化百分比和取代度分别为 2.48% 和 0.01,符合现行规定。MS 的直链淀粉含量(23.62 ± 1.89%)高于 NS(16.01 ± 0.43%)。MS 的 Tg 值降低,傅立叶变换红外光谱中 1012 和 1723 cm-1 处出现的条带表明乙酰化改变了淀粉的功能特性。2% 和 3% 的 MS 乳液(MS2 和 MS3)显示出较小的液滴尺寸和较高的界面分散性。不过,MS3 的粘度更高,导致疏水性增加,絮凝和随后的凝聚延迟。这项研究为在新食品配方中使用 3% MS 敷料提供了有用的信息,在降低脂肪含量的同时保留了功能特性,从而确保了更高的稳定性。
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引用次数: 0
Effect of meat type and fat level on burger meat characteristics: A combined approach using imaging and sensory techniques. 肉类和脂肪含量对汉堡肉特征的影响:使用成像和感官技术的综合方法。
IF 1.8 4区 农林科学 Q3 CHEMISTRY, APPLIED Pub Date : 2024-11-14 DOI: 10.1177/10820132241297672
María Cardona, Jose M Barat, Isabel Fernández-Segovia, Raúl Grau

This study aimed to evaluate the effect of fat level and meat cut type on burger meat through color, texture, image, and sensory analyses, and to explore the ability of the imaging technique as a complementary tool for consumer quality perception. For this purpose, burger meat samples were prepared by combining pork and beef meat (50/50%) with other nonmeat ingredients. The differences between samples were fat level: around 15 g fat/100 g (code HF) or 10.5 g fat/100 g (code LF); cut types: from cow carcasses (code C) or yearling (code Y). Instrumental color and texture measures analysis, an image analysis, and a sensory evaluation were carried out on samples (raw and cooked) at two times: day 0 and day 1 before expiry dates. The results showed that the samples made with meat from cows presented lower L* and higher a* and b* values than the samples made from yearling. However, the fat level did not affect this parameter. This same pattern was observed for the image measurements. Regarding texture, the samples with higher fat content (and lower moisture content) had higher hardness values. Generally, meat type had no effect on textural parameters. The samples that consumers gave the highest overall acceptance scores were those made of cow meat at both fat content levels. These samples also had the highest raw and cooked color scores. The fat level slightly affected hardness, with the same pattern observed for the instrumental measures of texture. The analyses showed a good correlation between instrumental techniques and sensory evaluation.

本研究旨在通过颜色、质地、图像和感官分析,评估脂肪含量和切肉类型对汉堡肉的影响,并探索成像技术作为消费者质量感知辅助工具的能力。为此,我们将猪肉和牛肉(各占 50%)与其他非肉类配料混合制成汉堡肉样品。样品之间的差异在于脂肪含量:约 15 克脂肪/100 克(代码 HF)或 10.5 克脂肪/100 克(代码 LF);切割类型:来自牛胴体(代码 C)或一岁牛(代码 Y)。在保质期前的第 0 天和第 1 天这两个时间段,对样品(生的和熟的)进行了色泽和质地测量分析、图像分析和感官评估。结果表明,用奶牛肉制作的样品比用一岁羔羊肉制作的样品的 L* 值低,a* 和 b* 值高。然而,脂肪含量并不影响这一参数。在图像测量中也观察到了同样的模式。在质地方面,脂肪含量较高(水分含量较低)的样品硬度值较高。一般来说,肉的种类对质地参数没有影响。消费者总体接受度得分最高的样品是两种脂肪含量水平下都由牛肉制成的样品。这些样品的生熟色泽得分也最高。脂肪含量对硬度略有影响,质地的仪器测量结果也是如此。分析表明,仪器技术与感官评价之间存在良好的相关性。
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引用次数: 0
Development and quality evaluation of a finger millet (Eleusine coracana (L.) Gaertn.) flour incorporated set yoghurt. 掺入酸奶的小米(Eleusine coracana (L.) Gaertn.)粉的开发和质量评价。
IF 1.8 4区 农林科学 Q3 CHEMISTRY, APPLIED Pub Date : 2024-11-12 DOI: 10.1177/10820132241297840
Sooriya Arachchige Sachini Jayawardana, Jayanetti Koralalage Ramani Radhika Samarasekera, Gardhi Hettiarachchige Chamari Madhu Hettiarachchi, Mahavidanage Jaanaki Gooneratne

Nutritional and therapeutic properties of yoghurts can be enhanced by incorporating bioactive food components. The present study was focused on formulating a finger millet flour incorporated set yoghurt to deliver the inherent health benefits of finger millet to consumers in a ready-to-eat way. Ten yoghurt samples were formulated incorporating different percentages of finger millet flour. Physicochemical and microbiological properties of the yoghurts were evaluated. When considering organoleptic properties, 7% finger millet flour incorporated yoghurt was selected as the most preferable yoghurt among the tested finger millet flour incorporated yoghurts. It was complying with the physicochemical requirements and microbiological limits mentioned in the Sri Lanka Standard - Specification for yoghurts. Incorporation of 7% finger millet flour enhanced the nutritional composition of the yoghurt in terms of total dietary fibers (1.04%), total phenolic content (54.09 mg Gallic acid equivalents/100 g of yoghurt), total flavonoid content (8.00 mg Quercetin equivalents/100 g of yoghurt) and antioxidant properties including ABTS radical scavenging activity (190.81 mg Trolox equivalents/100 g of yoghurt) without affecting the sensory properties. Since it was formulated without adding gelatine, the 7% finger millet flour incorporated yoghurt is suitable for lacto-vegetarians. When stored in a refrigerator at 4 °C, the 7% finger millet flour incorporated yoghurt could be stored satisfactorily for four weeks. The finger millet flour incorporated yoghurt is capable of not only fulfilling the consumer demands for finger millet-based ready-to-eat foods and diversified yoghurt products, but also delivering additional health benefits, beyond the basic nutritional functions of a yoghurt, to consumers.

通过加入生物活性食品成分,可以增强酸奶的营养和治疗特性。本研究的重点是配制掺入小米粉的套装酸奶,以即食方式向消费者提供小米固有的健康益处。研究人员配制了十种含有不同比例小米粉的酸奶样品。对酸奶的理化和微生物特性进行了评估。考虑到感官特性,在测试的掺入了小米粉的酸奶中,7% 的掺入小米粉酸奶被选为最受欢迎的酸奶。它符合《斯里兰卡标准--酸奶规范》中提到的理化要求和微生物限度。在不影响感官特性的情况下,添加 7% 的小米粉提高了酸奶的营养成分,包括总膳食纤维(1.04%)、总酚含量(54.09 毫克没食子酸当量/100 克酸奶)、总黄酮含量(8.00 毫克槲皮素当量/100 克酸奶)和抗氧化特性,包括 ABTS 自由基清除活性(190.81 毫克三环氧化酶当量/100 克酸奶)。由于在配制过程中没有添加明胶,因此添加了 7% 小米粉的酸奶适合乳素食者食用。将含 7% 小米粉的酸奶储存在 4 ℃ 的冰箱中,可存放四周,效果令人满意。加入小米粉的酸奶不仅能满足消费者对以小米为基础的即食食品和多样化酸奶产品的需求,还能为消费者带来酸奶基本营养功能之外的额外健康益处。
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引用次数: 0
Proximate, structural, textural, sensory and microbiological properties of non-gluten extrudate using Sorghum (Sorghum bicolor L. Moench) and a sprouted legume (Phaseolus lunatus L.). 使用高粱(Sorghum bicolor L. Moench)和发芽豆科植物(Phaseolus lunatus L.)制成的非面筋挤压物的近似物、结构、质地、感官和微生物特性。
IF 1.8 4区 农林科学 Q3 CHEMISTRY, APPLIED Pub Date : 2024-10-14 DOI: 10.1177/10820132241289157
Adedoyin I Adebayo, Adebola O Oladunjoye

The inclusion of legumes as functional ingredients in a gluten-free extrusion process has been gaining attention in recent times. In this study, sorghum and germinated lima bean flour (100, 90:10, 80:20, 70:30, 60:40 and 50:50) was extruded (feed moisture - 18%, screw speed - 250 rpm, barrel temperatures 50 °C-120 °C-120 °C, die hole diameter - 3 mm) and analysed for functional, proximate, textural, structural, pasting, microbiological and sensory properties. With 100% sorghum used as control, lima beans addition significantly (p < 0.05) improved the loose (0.37-0.44 g/ml) and packed (0.63-0.72 g/ml) bulk density, while water (5.00-3.15 g/g) and oil (2.45-1.60 g/g) absorption capacity and expansion ratio (3.11-2.30) decreased, respectively. An increase in protein (12.77-18.00%), crude fibre (2.58-5.17%) and ash content (2.11-3.12%) were observed in the extrudate, while the (L*) colour parameter (54.49-43.62), hardness (180.04-78.36 N) and pasting viscosities reduced with addition of lima beans. The structural micrograph depicted air-trapped bubbles with thick walls after adding lima beans, while a notable decline in microbial count below approved limits was observed after 8 weeks of storage. Sensory scores showed that values obtained were above average with the 90:10 sorghum-lima bean ratio having the highest score. The economic and industrial value of underutilised legumes such as lima bean can be promoted as functional ingredients via extrusion in addressing coeliac disease and alternative sources of protein, especially in developing countries.

近来,在无麸质挤压工艺中加入豆类作为功能性配料越来越受到关注。在这项研究中,对高粱和发芽利马豆粉(100、90:10、80:20、70:30、60:40 和 50:50)进行了挤压(饲料水分 - 18%,螺杆转速 - 250 rpm,机筒温度 50 °C-120 °C-120 °C,模孔直径 - 3 mm),并对其功能、近似物、质地、结构、糊化、微生物和感官特性进行了分析。以 100% 高粱为对照,添加利马豆后,色泽参数(54.49-43.62)、硬度(180.04-78.36 牛顿)和糊化粘度均显著降低(p L*)。结构显微照片显示,添加利马豆后,气泡壁厚,而微生物数量在储存 8 周后显著下降,低于批准的限值。感官评分显示,所得数值高于平均值,其中高粱与利马豆的比例为 90:10,得分最高。利马豆等未充分利用的豆类的经济和工业价值可通过挤压作为功能性配料加以推广,以解决乳糜泻和替代蛋白质来源的问题,尤其是在发展中国家。
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引用次数: 0
Development of an exposure assessment industrial model for Bacillus cereus in rice matrix containing insect chitosan. 开发含有昆虫壳聚糖的大米基质中蜡样芽孢杆菌暴露评估工业模型。
IF 1.8 4区 农林科学 Q3 CHEMISTRY, APPLIED Pub Date : 2024-10-14 DOI: 10.1177/10820132241289325
María Inés Valdez-Narváez, M Teresa Fernández-Felipe, Dolores Rodrigo

An exposure assessment model for industrial use has been developed by using kinetic data from inactivation and growth of Bacillus cereus spores. It can provide a valuable tool for estimating the concentration of B. cereus after a storage period of 24 h at a specified temperature (20 °C) and for an estimation of the percentage of contaminated portions according to the input data of the model. This model considers a rice-derived product that has undergone a standard cooking process at 95 °C for 20 min. According to the results, the presence of chitosan affects the final microbial load after storage, potentially serving as an additional control measure in the event of cold chain abuse or break. Chitosan's antimicrobial properties likely play a role in reducing microbial growth during storage, thereby contributing to enhanced food safety. In practical terms, this suggests that incorporating chitosan into food products, especially those susceptible to microbial contamination like rice derivatives, could help mitigate risks associated with temperature abuse or cold chain disruptions. By acting as a protective barrier against microbial proliferation, chitosan offers a preventive measure to maintain product quality and safety throughout the supply chain. Considering two scenarios, 104 or 107 as initial contamination the model estimated that the 55 and 100% of portions would be respectively contaminated, according to a Performance Criteria of 4 log reductions.

利用蜡样芽孢杆菌孢子灭活和生长的动力学数据,开发了一种用于工业用途的暴露评估模型。该模型提供了一个宝贵的工具,用于估算在指定温度(20 °C)下储存 24 小时后蜡样芽孢杆菌的浓度,并根据模型的输入数据估算受污染部分的百分比。该模型考虑的是经过 95 °C 20 分钟标准蒸煮过程的大米产品。结果表明,壳聚糖的存在会影响贮藏后的最终微生物量,有可能在冷链滥用或断裂的情况下作为额外的控制措施。壳聚糖的抗菌特性可能会在贮藏过程中起到减少微生物生长的作用,从而有助于提高食品安全。在实际应用中,这表明将壳聚糖添加到食品中,尤其是大米衍生物等易受微生物污染的食品中,有助于降低与温度滥用或冷链中断相关的风险。壳聚糖可作为防止微生物扩散的保护屏障,为在整个供应链中保持产品质量和安全提供预防措施。考虑到 104 或 107 作为初始污染的两种情况,根据减少 4 个对数的性能标准,该模型估计分别有 55% 和 100% 的部分会受到污染。
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引用次数: 0
Solid state fermentation of mung beans by Bacillus subtilis subsp. natto on static, shaking flask and soft elastic tubular reactors. 纳豆芽孢杆菌在静态、摇瓶和软弹性管式反应器上对绿豆进行固态发酵。
IF 1.8 4区 农林科学 Q3 CHEMISTRY, APPLIED Pub Date : 2024-10-01 Epub Date: 2023-03-13 DOI: 10.1177/10820132231162167
He Lyu, Saartje Hernalsteens, Haihua Cong, S-Y Quek, Xiao Dong Chen

Given that mung beans constitute a significant nutrient source in many cultures, it is worthwhile to investigate ways to improve their nutritional and functional properties. The effect of fermentation of mung beans by Bacillus subtilis subsp. natto was investigated in various reactor designs, including static, shaking flasks, and soft elastic tubular reactors (SETR). The results showed that all three processes might affect the substrate, resulting in changes in the protein and carbohydrate fractions. We noticed an increase in soluble protein and serine levels, which we attribute to the proteases produced during fermentation. Through XRD, FTIR, and DSC analyses, it was also discovered that whereas static and shaking flask fermentation might raise relative crystallinity and peak temperature, fermentation performed on the SETR decreased these values. It was also possible to notice that SETR might induce a change in the particle size distribution of the substrate through a complex impact of mechanical forces, mixing, and microbial activity, which could be helpful to some aspects of the process. To summarize, fermentation of mung beans by Bacillus. subtilis subsp. natto could be an attractive approach for producing a food ingredient with various functional and nutritional properties. Furthermore, the SETR has been shown to be a viable technique for dealing with high solid load substrates, whether as the reactor for the entire process or as a first stage/pre-treatment step, and its applicability in bioprocesses should be explored further.

鉴于绿豆是许多文化中的重要营养来源,因此值得研究如何改善其营养和功能特性。研究人员在不同的反应器设计(包括静态反应器、振荡烧瓶和软弹性管式反应器(SETR))中研究了枯草芽孢杆菌纳豆亚种对绿豆发酵的影响。结果表明,这三个过程都可能影响底物,导致蛋白质和碳水化合物组分发生变化。我们注意到可溶性蛋白质和丝氨酸含量的增加,这归因于发酵过程中产生的蛋白酶。通过 XRD、FTIR 和 DSC 分析,我们还发现,静态发酵和摇瓶发酵可能会提高相对结晶度和峰值温度,而在 SETR 上进行的发酵则会降低这些值。此外,还发现 SETR 可能会通过机械力、混合和微生物活动的复杂影响,引起基质粒度分布的变化,这可能对工艺的某些方面有所帮助。总之,用枯草芽孢杆菌纳豆亚种发酵绿豆是生产具有各种功能和营养特性的食品配料的一种有吸引力的方法。此外,无论是作为整个过程的反应器,还是作为第一阶段/预处理步骤,SETR 已被证明是处理高固体负荷基质的可行技术,其在生物过程中的适用性有待进一步探索。
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Food Science and Technology International
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