Pub Date : 2019-06-24DOI: 10.1364/COSI.2019.JW2A.4
M. Bathe-Peters, P. Gmach, P. Annibale, M. Lohse
We present the setup of a fluorescence microscope combining TIRF and confocal microscopy within one body to address multi-scale dynamics of GPCRs on plasma membranes of living cells with adequate spatial and temporal resolution.
{"title":"All-in-one confocal and widefield fluorescence microscope to investigate membrane receptor dynamics","authors":"M. Bathe-Peters, P. Gmach, P. Annibale, M. Lohse","doi":"10.1364/COSI.2019.JW2A.4","DOIUrl":"https://doi.org/10.1364/COSI.2019.JW2A.4","url":null,"abstract":"We present the setup of a fluorescence microscope combining TIRF and confocal microscopy within one body to address multi-scale dynamics of GPCRs on plasma membranes of living cells with adequate spatial and temporal resolution.","PeriodicalId":123636,"journal":{"name":"Imaging and Applied Optics 2019 (COSI, IS, MATH, pcAOP)","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-06-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115390309","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-06-24DOI: 10.1364/COSI.2019.CTU4A.3
Xiaohan Li, J. Greenberg, M. Gehm
Memory effect (ME) imaging forms high-fidelity, diffraction-limited images through scatter without any prior knowledge. Here we demonstrate compressive ME imaging by extracting a color image of a scatter-obscured object from a single coded monochrome exposure.
{"title":"Single-shot Multispectral Color Imaging Through Scattering Media","authors":"Xiaohan Li, J. Greenberg, M. Gehm","doi":"10.1364/COSI.2019.CTU4A.3","DOIUrl":"https://doi.org/10.1364/COSI.2019.CTU4A.3","url":null,"abstract":"Memory effect (ME) imaging forms high-fidelity, diffraction-limited images through scatter without any prior knowledge. Here we demonstrate compressive ME imaging by extracting a color image of a scatter-obscured object from a single coded monochrome exposure.","PeriodicalId":123636,"journal":{"name":"Imaging and Applied Optics 2019 (COSI, IS, MATH, pcAOP)","volume":"52 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-06-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121020456","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-06-24DOI: 10.1364/ISA.2019.ITU2C.5
Dukho Lee, Seokil Moon, Byounghyo Lee, Byoun-gkil Lee
In this paper, we propose a method to increase the resolution of holographic display using LED. The method increases the spatial coherence of the reference wave for holographic display. The proposed method is experimentally verified.
{"title":"A Method for Increasing the Resolution of Holographic Display Using LED","authors":"Dukho Lee, Seokil Moon, Byounghyo Lee, Byoun-gkil Lee","doi":"10.1364/ISA.2019.ITU2C.5","DOIUrl":"https://doi.org/10.1364/ISA.2019.ITU2C.5","url":null,"abstract":"In this paper, we propose a method to increase the resolution of holographic display using LED. The method increases the spatial coherence of the reference wave for holographic display. The proposed method is experimentally verified.","PeriodicalId":123636,"journal":{"name":"Imaging and Applied Optics 2019 (COSI, IS, MATH, pcAOP)","volume":"5 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-06-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125051136","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-06-24DOI: 10.1364/COSI.2019.CM2A.5
D. Lin, J. Leger, Connor Hashemi
We present a passive technique for recovering the conventional imagery of a non-line- of-sight scene using the spatial and angular components of the scattered light field along a homogeneous surface.
我们提出了一种利用均匀表面散射光场的空间和角度分量来恢复非视线场景的传统图像的被动技术。
{"title":"Non-Line-of-Sight Imaging using Plenoptic Information","authors":"D. Lin, J. Leger, Connor Hashemi","doi":"10.1364/COSI.2019.CM2A.5","DOIUrl":"https://doi.org/10.1364/COSI.2019.CM2A.5","url":null,"abstract":"We present a passive technique for recovering the conventional imagery of a non-line- of-sight scene using the spatial and angular components of the scattered light field along a homogeneous surface.","PeriodicalId":123636,"journal":{"name":"Imaging and Applied Optics 2019 (COSI, IS, MATH, pcAOP)","volume":"98 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-06-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"116074255","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-06-24DOI: 10.1364/COSI.2019.JW2A.7
Penghuan Liu
We can not achieve a higher resolution in blind structured illumination microscopy by substituting fully developed speckle with saturated speckle illuminations.
在盲目结构照明显微镜中，用饱和散斑照明代替完全发育的散斑照明无法获得更高的分辨率。
{"title":"Blind structured illumination microscopy using saturated speckle patterns","authors":"Penghuan Liu","doi":"10.1364/COSI.2019.JW2A.7","DOIUrl":"https://doi.org/10.1364/COSI.2019.JW2A.7","url":null,"abstract":"We can not achieve a higher resolution in blind structured illumination microscopy by substituting fully developed speckle with saturated speckle illuminations.","PeriodicalId":123636,"journal":{"name":"Imaging and Applied Optics 2019 (COSI, IS, MATH, pcAOP)","volume":"22 5 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-06-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123018471","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-06-24DOI: 10.1364/COSI.2019.CTU4C.6
A. Khalid, A. Ray, A. Demčenko, Steve Cohen, M. Tassieri, J. Reboud, A. Ozcan, J. Cooper
Integration of acoustically actuated nanolenses into a lens-free microscopy enables a cost-effective platform for detection of nanoparticles and nanoliter volume rheology. We demonstrate the detection of single nanoparticles and the rheological analysis of viscoelastic fluids.
{"title":"Lens-free Microscopy Using Acoustically Actuated Nanolenses and its Applications","authors":"A. Khalid, A. Ray, A. Demčenko, Steve Cohen, M. Tassieri, J. Reboud, A. Ozcan, J. Cooper","doi":"10.1364/COSI.2019.CTU4C.6","DOIUrl":"https://doi.org/10.1364/COSI.2019.CTU4C.6","url":null,"abstract":"Integration of acoustically actuated nanolenses into a lens-free microscopy enables a cost-effective platform for detection of nanoparticles and nanoliter volume rheology. We demonstrate the detection of single nanoparticles and the rheological analysis of viscoelastic fluids.","PeriodicalId":123636,"journal":{"name":"Imaging and Applied Optics 2019 (COSI, IS, MATH, pcAOP)","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-06-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"129178799","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-06-24DOI: 10.1364/COSI.2019.CW4A.3
S. Ludwig, B. Le Teurnier, G. Pedrini, A. Herkommer, W. Osten
Scatter-plate microscopy is a lensless imaging method using a simple groundglass diffuser instead of a microscope objective. In this paper we describe further application of deconvolution improving both contrast and resolution of the acquired images.
{"title":"Deconvolution in Scatter-plate Microscopy","authors":"S. Ludwig, B. Le Teurnier, G. Pedrini, A. Herkommer, W. Osten","doi":"10.1364/COSI.2019.CW4A.3","DOIUrl":"https://doi.org/10.1364/COSI.2019.CW4A.3","url":null,"abstract":"Scatter-plate microscopy is a lensless imaging method using a simple groundglass diffuser instead of a microscope objective. In this paper we describe further application of deconvolution improving both contrast and resolution of the acquired images.","PeriodicalId":123636,"journal":{"name":"Imaging and Applied Optics 2019 (COSI, IS, MATH, pcAOP)","volume":"12 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-06-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121792970","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-06-24DOI: 10.1364/COSI.2019.CTH3A.2
Ruslan Röhrich, Chris Hoekmeijer, C. I. Osorio, A. Koenderink
We report an experimental technique for quantitative analysis of amplitude, phase, directivity and polarization of wavefronts scattered by single nano-objects. As a demonstration we quantify the spin and orbital angular momentum imposed by plasmonic spiral nano-antennas.
{"title":"Polarimetric and interferometric measurement of orbital angular momentum imparted by single plasmon nano-antennas","authors":"Ruslan Röhrich, Chris Hoekmeijer, C. I. Osorio, A. Koenderink","doi":"10.1364/COSI.2019.CTH3A.2","DOIUrl":"https://doi.org/10.1364/COSI.2019.CTH3A.2","url":null,"abstract":"We report an experimental technique for quantitative analysis of amplitude, phase, directivity and polarization of wavefronts scattered by single nano-objects. As a demonstration we quantify the spin and orbital angular momentum imposed by plasmonic spiral nano-antennas.","PeriodicalId":123636,"journal":{"name":"Imaging and Applied Optics 2019 (COSI, IS, MATH, pcAOP)","volume":"120 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-06-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"133996183","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
S. Panigrahi, J. Fade, R. Agaisse, H. Ramachandran, M. Alouini
We report the design and experimental proof-of-principle of a novel snapshot quadrature demodulation imaging architecture, allowing retrieval of amplitude and phase of light modulated at radio frequencies, without synchronization between emitter and receiver, and with continuous frequency tuning capability.
{"title":"All-optical imaging architecture for snapshot demodulation of optical signals at radio-frequencies","authors":"S. Panigrahi, J. Fade, R. Agaisse, H. Ramachandran, M. Alouini","doi":"10.1364/ISA.2019.IW3B.1","DOIUrl":"https://doi.org/10.1364/ISA.2019.IW3B.1","url":null,"abstract":"We report the design and experimental proof-of-principle of a novel snapshot quadrature demodulation imaging architecture, allowing retrieval of amplitude and phase of light modulated at radio frequencies, without synchronization between emitter and receiver, and with continuous frequency tuning capability.","PeriodicalId":123636,"journal":{"name":"Imaging and Applied Optics 2019 (COSI, IS, MATH, pcAOP)","volume":"60 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-06-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134299185","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-06-24DOI: 10.1364/ISA.2019.ITH3B.2
Lucien E. Weiss, Boris Ferdman, Onit Alalouf, Y. Shechtman
By imaging the back focal plane of a fluorescence microscope, we demonstrate precise determination of a sample’s refractive index in a fluorophore-laden microfluidic device. We apply the technique to biosensing by detecting unlabeled bacteria.
{"title":"Label-Free Biosensing by Ultrasensitive Supercritical Angle Refractometry","authors":"Lucien E. Weiss, Boris Ferdman, Onit Alalouf, Y. Shechtman","doi":"10.1364/ISA.2019.ITH3B.2","DOIUrl":"https://doi.org/10.1364/ISA.2019.ITH3B.2","url":null,"abstract":"By imaging the back focal plane of a fluorescence microscope, we demonstrate precise determination of a sample’s refractive index in a fluorophore-laden microfluidic device. We apply the technique to biosensing by detecting unlabeled bacteria.","PeriodicalId":123636,"journal":{"name":"Imaging and Applied Optics 2019 (COSI, IS, MATH, pcAOP)","volume":"80 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-06-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130833674","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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